Cationically substituted diphenylazetidinones, process for their preparation, medicaments comprising these compounds, and their use

Abstract

One embodiment of the invention relates to compounds of the formula I, 1

Description

[0001] This application claims the benefit of the filing dates of German Patent Application Number 10227507.6, filed on Jun. 19, 2002, and U.S. Provisional Application No. 60/411,981, filed on Sep. 19, 2002, which applications are hereby incorporated by reference.

[0002] One embodiment of the invention relates to cationically substituted diphenylazetidinones, their physiologically acceptable salts and derivatives having physiological functions.

[0003] Diphenylazetidinones (such as, for example, ezetimibe) and their use for treating hyperlipidemia, arteriosclerosis and hypercholesterolemia have already been described [cf. Drugs of the Future 2000, 25(7):679-685 and U.S. Pat. No. 5,756,470].

[0004] One embodiment of the invention provides further compounds having a therapeutically utilizable hypolipidemic action. For example, one embodiment of the invention relates to novel compounds which, compared to the compounds described in the prior art, are absorbed to a very low extent. Very low absorption is to be understood as meaning an intestinal absorption of less than about 10%, for example less than or equal to about 5%.

[0005] In one embodiment, absorption of the novel compounds of the invention may be less than that of ezetimibe.

[0006] In general, pharmaceutically active compounds that are absorbed to a low extent may have considerably fewer side-effects.

[0007] Accordingly, one embodiment of the invention relates to compounds of the formula I 2

[0008] wherein

[0009] R1, R2, R3, R4, R5, and R6, independently of one another, are chosen from:

[0010] (C0-C30)-alkylene-(LAG)q; or

[0011] (C0-C30)-alkylene-(LAG)q, wherein one or more carbon atoms of the alkylene radical may be replaced by a radical chosen from: —S(O)m-(wherein m=0-2), —O—, —(C═O)—, —(C═S)—, —CH═CH—, —C≡C—, —N((C1-C6)-alkyl)—, —N(phenyl), —N((C1-C6)-alkyl-phenyl)—, —N(CO— (CH2)1-10—COOH)— and —NH—; or

[0012] H, F, Cl, Br, I, CF3, NO2, N3, CN, COOH, COO(C1-C6)-alkyl, CONH2, CONH(C1-C6)-alkyl, CON[(C1-C6)-alkyl]2, (C1-C6)-alkyl, (C2-C6)-alkenyl, (C2-C6)-alkynyl, O—(C1-C6)-alkyl, wherein the alkyl radical is unsubstituted or at least one hydrogen in the alkyl radical is replaced by fluorine; or

[0013] C(═NH)(NH2), PO3H2, SO3H, SO2—NH2, SO2NH(C1-6)-alkenyl, SO2N[(C1-C6)-alkyl]2, S—(C1-C6alkyl, S—(CH2)n-phenyl, SO—(C1-C6)-alkyl, SO—(CH2)n-phenyl, SO2—(C1-C6)-alkyl, SO2—(CH2)n-phenyl,

[0014] wherein n=0-6, and wherein the phenyl radical is unsubstituted or substituted one or two times, each substituent chosen independently from: F, Cl, Br, OH, CF3, NO2, CN, OCF3, O—(C1-C6)-alkyl, (C1-C6)-alkyl, and NH2; or

[0015] NH2, NH—(C1-C6)-alkyl, N((C1-C6)-alkyl)2, NH(C1-C7)-acyl, phenyl, O—(CH2)n-phenyl, wherein n=0-6, and wherein the phenyl ring is unsubstituted or substituted one, two, or three times, each substituent chosen independently from: F, Cl, Br, I, OH, CF3, NO2, CN, OCF3, O—(C1-C6)-alkyl, (C1-C6)-alkyl, NH2, NH(C1-C6)-alkyl, N((C1-C6)-alkyl)2, SO2—CH3, COOH, COO—(C1-C6)-alkyl, or CONH2;

[0016] wherein (LAG) is a mono-, di- or tricyclic trialkylammonium radical, a mono-, di- or tricyclic trialkylammoniumalkyl radical, —(CH2)0-10—C(═NH)(NH2); —(CH2)0-10—C(═NH)(NHOH) or —NR7—C(═NR8)(NR9R10) and wherein R7, R8, R9 and R10, independently of one another, are chosen from: H, (C1-C6)-alkyl, (C1-C6)-alkyl-phenyl, phenyl, and (C3-C8)-cycloalkyl, and wherein q is 1-5,

[0017] wherein at least one of the radicals R1 to R6 must have the meaning:

[0018] (C0-C30)-alkylene-(LAG)q; or

[0019] (C0-C30)-alkylene-(LAG)q, wherein at least one carbon atom of the alkylene radical is replaced by a radical chosen from: —S(O)m— (wherein m =0-2), —O—, —(C═O)—, —(C═S)—, —CH═CH—, —C≡C—, —N((C1-C6)-alkyl)—, —N(phenyl)—, —N((C1-C6)-alkyl-phenyl)—, —N(CO—(CH2)1-10—COOH)— and —NH—;

[0020] or a pharmaceutically acceptable salt thereof, in any stereoisomeric form, or a mixture of any such compounds in any ratio.

[0021] In one embodiment of the invention, at least one of the radicals R1 to R6 in the compounds of the formula I has the meaning (C0-C30)-alkylene-(LAG)q; or (C0-C30)-alkylene-(LAG)q, wherein at least one carbon atom of the alkylene radical is replaced by a radical chosen from: —O—, —(C═O)—, —N((C1-C6)-alkyl)—, —N(CO—(CH2)1-10—COOH)—and —NH—.

[0022] In another embodiment of the invention, one of the radicals R1 or R3 in the compounds of the formula I has the meaning —(C0-C30)-alkylene-(LAG); or (C0-C30)-alkylene-(LAG), wherein at least one carbon atom of the alkylene radical may be replaced by —O—, —(C═O)—, —N(CH3)— or —NH—.

[0023] In another embodiment of the invention, one of the radicals R1 or R3 in the compounds of the formula I has the meaning —(CH2)0-1—Y—W—(C0-C25)-alkylene-Y′—W′—(LAG); or —(CH2)0-1—Y—W—(C0-C25)-alkylene-Y′—W′—(LAG), wherein at least one carbon atom of the alkylene radical is replaced by an oxygen atom; and wherein Y and W, independently of one another, are chosen from: NH, NCH3, C═O, O, a bond, and S(O)m, wherein m=0-2, and Y′ and W′, independently of one another, are chosen from: NH, NCH3, C═O, O, a bond or S(O)m, wherein m=0-2; or Y—W or Y′—W′ in each case, together represent a bond.

[0024] In another embodiment of the invention, the group LAG in any of the radicals R1 to R6 in the compounds of the formula I is a dicyclic dialkylammoniumalkyl radical.

[0025] A mono-, di- or tricyclic trialkylammonium radical is to be understood as meaning, for example, radicals such as 3

[0026] wherein n, m and p, independently of one another, can be 0-10 and wherein one or more CH2 groups independently of one another may be replaced by a radical chosen from: O, S(O)m (wherein m may be 0-2), NH, N—(C1-C10)-alkyl, N-phenyl, and N—CH2-phenyl.

[0027] A mono-, di- or tricyclic trialkylammoniumalkyl radical is to be understood as meaning, for example, radicals such as 4

[0028] wherein n, m and p, independently of one another, can be 0-10 and wherein one or more CH2 groups, independently of one another, may be replaced by O, S(O)m (wherein m may be 0-2), NH, N—(C1-C10)-alkyl, N-phenyl or N—CH2-phenyl and Alk1 is a straight-chain or branched alkyl radical having 1 to 20 carbon atoms.

[0029] Other than in the operating examples, or where otherwise indicated, all numbers expressing quantities of ingredients, reaction conditions, and so forth used in the specification and claims are to be understood as being modified in all instances by the term “about.” Accordingly, unless indicated to the contrary, the numerical parameters set forth in the present specification and attached claims are approximations that may vary depending upon the desired properties sought to be obtained by the present invention. At the very least, and not as an attempt to limit the application of the doctrine of equivalents to the scope of the claims, each numerical parameter should be construed in light of the number of reported significant digits and by applying ordinary rounding techniques.

[0030] Notwithstanding that the numerical ranges and parameters setting forth the broad scope of the invention are approximations, the numerical values set forth in the specific examples are reported as precisely as possible. Any numerical value, however, inherently contain certain errors necessarily resulting from the standard deviation found in their respective testing measurements.

[0031] Owing to their increased solubility in water, pharmaceutically acceptable salts are often more suitable for medical applications than the parent compounds.

[0032] These salts generally have a pharmaceutically acceptable anion or cation. Examples of suitable pharmaceutically acceptable acid addition salts of the compounds according to the invention include salts of inorganic acids, such as hydrochloric acid, hydrobromic acid, phosphoric acid, metaphosphoric acid, nitric acid, sulfonic acid and sulfuric acid, and of organic acids, such as acetic acid, benzenesulfonic acid, benzoic acid, citric acid, ethanesulfonic acid, fumaric acid, gluconic acid, glycolic acid, isothionic acid, lactic acid, lactobionic acid, maleic acid, malic acid, methanesulfonic acid, succinic acid, p-toluenesulfonic acid, tartaric acid and trifluoroacetic acid, for example. An example of an acceptable salt of the compounds of the invention is the chloride salt. Examples of suitable pharmaceutically acceptable basic salts include ammonium salts, alkali metal salts (such as sodium and potassium salts) and alkaline earth metal salts (such as magnesium and calcium salts).

[0033] The scope of the invention also includes salts having a pharmaceutically unacceptable anion, which salts may be useful intermediates for preparing or purifying pharmaceutically acceptable salts and/or for use in nontherapeutic, for example in vitro, applications.

[0034] Here, the term “derivative having physiological function” refers to any physiologically acceptable derivative of a compound according to the invention, for example an ester, that is able, upon administration to a mammal, for example a human, to form such a compound or an active metabolite (directly or indirectly).

[0035] A further aspect of this invention includes prodrugs of the compounds according to the invention. Such prodrugs can be metabolized in vivo to give a compound according to the invention. These prodrugs may or may not be active in their own right.

[0036] The compounds according to the invention can also be present in various polymorphic forms, for example as amorphous and crystalline polymorphous forms.

[0037] Accordingly, another aspect of the invention includes the polymorphic forms of the compounds according to the invention.

[0038] Hereinbelow, all references to “compound(s) of the formula (I)” refer to a compound or compounds of the formula (I) as described above, and to their salts, solvates and derivatives having physiological function, as described herein.

[0039] The compounds of the formula I and their pharmaceutically acceptable salts and derivatives having physiological function are useful medicaments for treating an impaired lipid metabolism, for example hyperlipidemia. The compounds of the formula I are also suitable for modulating the serum cholesterol concentration and for preventing and treating arteriosclerotic manifestations.

[0040] As used herein, treating or treatment includes the treating of, for example, a patient inflicted with a disease or condition, as well as the prevention, prophylaxis, or protective treatment of a patient. Treatment also includes treating a subject susceptible to or predisposed to developing a disease or condition, which could include patients in whom the disease or condition has not yet presented, as well as patients in whom the disease has been successfully treated but could redevelop or reoccur.

[0041] The compound(s) of the formula (I) can also be administered in combination with other active compounds.

[0042] The amount of a compound of the formula (I) required to achieve the desired biological effect depends on a number of factors, for example on the specific compound chosen, on the intended use, on the mode of administration and on the clinical condition of the patient. In general, the daily dose is in the range from 0.1 mg to 100 mg (typically from 0.1 mg to 50 mg) per day per kilogram of bodyweight, for example 0.1-10 mg/kg/day. Tablets or capsules may contain, for example, from 0.01 to 100 mg, typically from 0.02 to 50 mg. In the case of pharmaceutically acceptable salts, the abovementioned weight data relate to the weight of the diphenylazetidinone-ion derived from the salt. An effective amount of a compound of the invention is an amount sufficient to bring about a desired effect. For example, in the context of treating an impaired lipid metabolism, for instance hyperlipidemia, an effective amount of a compound of the invention would constitute an amount sufficient to bring about a beneficial change in the condition of the patient. For the prophylaxis or therapy of the abovementioned conditions, the compounds of the formula (I) can be used by themselves, but they may also be present in the form of a pharmaceutical composition with an acceptable carrier. The carrier must of course be acceptable in the sense that it is compatible with the other constituents of the composition and relatively speaking is not harmful to the health of the patient. The carrier can be a solid or a liquid or both and may be formulated with the compound as an individual dose, for example as a tablet, which can contain from 0.05% to 95% by weight of the active compound. Further pharmaceutically active substances can also be present, including further compounds of the formula (I). The pharmaceutical compositions according to the invention can be prepared by one of the known pharmaceutical methods, which essentially consist in mixing the constituents with pharmacologically acceptable carriers and/or auxiliaries.

[0043] Pharmaceutical compositions according to the invention include those which are suitable for oral or peroral (e.g. sublingual) administration, although the most suitable manner of administration is dependent in each individual case on the nature and severity of the condition to be treated and on the type of the compound of the formula (I) used in each case. Coated formulations and coated delayed-release formulations are also included in the scope of the invention, as areacid-resistant and enteric formulations. Examples of suitable enteric coatings include cellulose acetate phthalate, polyvinyl acetate phthalate, hydroxypropylmethylcellulose phthalate and anionic polymers of methacrylic acid and methyl methacrylate.

[0044] Suitable pharmaceutical compounds for oral administration can be present in separate units, such as, for example, capsules, cachets, lozenges or tablets, which in each case contain a specific amount of the compound of the formula (I); as a powder or granules; as a solution or suspension in an aqueous or nonaqueous liquid; or as an oil-in-water or water-in-oil emulsion. As already mentioned, these compositions can be prepared according to any suitable pharmaceutical method that includes a step in which the active compound and the carrier (which can consist of one or more additional constituents) are brought into contact. In general, the compositions are prepared by uniform and homogeneous mixing of the active compound with a liquid and/or finely divided solid carrier, after which the product, if necessary, is shaped. For example, a tablet can thus be prepared by pressing or shaping a powder or granules of the compound, if appropriate with one or more additional constituents. Pressed tablets can be produced by tableting the compound in free-flowing form, such as, for example, a powder or granules, if appropriate mixed with a binder, lubricant, inert diluent and/or a (number of) surface-active/dispersing agent(s) in a suitable machine. Shaped tablets can be produced by shaping the pulverulent compound moistened with an inert liquid diluent in a suitable machine.

[0045] Pharmaceutical compositions suitable for peroral (sublingual) administration include lozenges which contain a compound of the formula (I) with a flavoring, customarily sucrose and gum arabic or tragacanth, and pastilles which include the compound in an inert base such as gelatin and glycerol or sucrose and gum arabic.

[0046] Other suitable active compounds for the combination preparations include, but are not limited to:

[0047] all antidiabetics mentioned in Rote Liste 2001, Chapter 12. They can be combined with the compounds of the formula I according to the invention to achieve a synergistically enhanced action. The active compound combination can be administered either by separate administration of the active compounds to the patient or in the form of combination preparations comprising a plurality of active compounds in a pharmaceutical preparation.

[0048] Antidiabetics include insulin and insulin derivatives, such as, for example, Lantus® or HMR 1964, GLP-1 derivatives, such as, for example, those disclosed by Novo Nordisk A/S in WO 98/08871, and oral hypoglycemic active compounds.

[0049] Examples of oral hypoglycemic active compounds include sulfonylureas, biguadines, meglitinides, oxadiazolidinediones, thiazolidinediones, glucosidase inhibitors, glucagon antagonists, GLP-1 agonists, potassium channel openers, such as, for example, those disclosed by Novo Nordisk A/S in WO 97/26265 and WO 99/03861, insulin sensitizers, inhibitors of liver enzymes involved in stimulating gluconeogenesis and/or glycogenolysis, modulators of glucose uptake, compounds which modulate lipid metabolism, such as antihyperlipidemic active compounds and antilipidemic active compounds, compounds that reduce food intake, PPAR and PXR agonists and active compounds that act on the ATP-dependent potassium channel of the beta cells.

[0050] In one embodiment of the invention, the compounds of the formula I are administered in combination with an HMGCoA reductase inhibitor such as simvastatin, fluvastatin, pravastatin, lovastatin, atorvastatin, cerivastatin, or rosuvastatin.

[0051] In another embodiment of the invention, the compounds of the formula I are administered in combination with a cholesterol absorption inhibitor, such as, for example, ezetimibe, tiqueside, or pamaqueside.

[0052] In another embodiment of the invention, the compounds of the formula I are administered in combination with a PPAR gamma agonist, such as, for example, rosiglitazone, pioglitazone, JTT-501, or GI 262570.

[0053] In another embodiment of the invention, the compounds of the formula I are administered in combination with a PPAR alpha agonist, such as, for example, GW 9578, or GW 7647.

[0054] In another embodiment of the invention, the compounds of the formula I are administered in combination with a mixed PPAR alpha/gamma agonist, such as, for example, GW 1536, AVE 8042, AVE 8134, or AVE 0847.

[0055] In another embodiment of the invention, the compounds of the formula I are administered in combination with a fibrate, such as, for example, fenofibrate, clofibrate, or bezafibrate.

[0056] In another embodiment of the invention, the compounds of the formula I are administered in combination with an MTP inhibitor, such as, for example, Bay 13-9952, BMS-201038, or R-103757.

[0057] In another embodiment of the invention, the compounds of the formula I are administered in combination with a bile acid absorption inhibitor, such as, for example, HMR 1453.

[0058] In another embodiment of the invention, the compounds of the formula I are administered in combination with a CETP inhibitor, such as, for example, Bay 194789.

[0059] In another embodiment of the invention, the compounds of the formula I are administered in combination with a polymeric bile acid adsorber, such as, for example, cholestyramine, or colesolvam.

[0060] In another embodiment of the invention, the compounds of the formula I are administered in combination with an LDL receptor inducer, such as, for example, HMR1171, or HMR1586.

[0061] In another embodiment of the invention, the compounds of the formula I are administered in combination with an ACAT inhibitor, such as, for example, avasimibe.

[0062] In another embodiment of the invention, the compounds of the formula I are administered in combination with an antioxidant, such as, for example, OPC-14117.

[0063] In another embodiment of the invention, the compounds of the formula I are administered in combination with a lipoprotein lipase inhibitor, such as, for example,

[0064] In another embodiment of the invention, the compounds of the formula I are administered in combination with an ATP citrate lyase inhibitor, such as, for example, SB-204990.

[0065] In another embodiment of the invention, the compounds of the formula I are administered in combination with a squalene synthetase inhibitor, such as, for example, BMS-188494.

[0066] In another embodiment of the invention, the compounds of the formula I are administered in combination with a lipoprotein(a) antagonist, such as, for example, Cl-1027 or nicotinic acid.

[0067] In another embodiment of the invention, the compounds of the formula I are administered in combination with a lipase inhibitor, such as, for example, Orlistat.

[0068] In another embodiment of the invention, the compounds of the formula I are administered in combination with insulin.

[0069] In another embodiment, the compounds of the formula I are administered in combination with a sulfonylurea, such as, for example, tolbutamide, glibenclamide, glipizide or gliclazide.

[0070] In another embodiment, the compounds of the formula I are administered in combination with a biguanide, such as, for example, metformin.

[0071] In another embodiment, the compounds of the formula I are administered a in combination with a meglitinide, such as, for example, repaglinide.

[0072] In another embodiment, the compounds of the formula I are administered in combination with a thiazolidinedione, such as, for example, troglitazone, ciglitazone, pioglitazone, rosiglitazone, or the compounds disclosed by Dr. Reddy's Research Foundation in WO 97/41097, for example 5-[[4-[(3,4-dihydro-3-methyl-4-oxo-2-quinazolinylmethoxy]phenyl]methyl]-2,4-thiazolidinedione.

[0073] In another embodiment, the compounds of the formula I are administered in combination with an α-glucosidase inhibitor, such as, for example, miglitol or acarbose.

[0074] In another embodiment, the compounds of the formula I are administered in combination with an active compound that acts on the ATP-dependent potassium channel of beta cells, such as, for example, tolbutamide, glibenclamide, glipizide, gliazide or repaglinide.

[0075] In another embodiment, the compounds of the formula I are administered in combination with more than one of the abovementioned compounds, for example in combination with a sulfonylurea and metformin, a sulfonylurea and acarbose, repaglinide and metformin, insulin and a sulfonylurea, insulin and metformin, insulin and troglitazon, insulin and lovastatin, etc.

[0076] In a further embodiment, the compounds of the formula I are administered in combination with CART agonists, NPY agonists, MC3 or MC4 agonists, orexin agonists, H3 agonists, TNF agonists, CRF agonists, CRF BP antagonists, urocortin agonists, β3-agonists, MCH (melanine-concentrating hormone) antagonists, CCK agonists, serotonin reuptake inhibitors, mixed serotonin and noradrenergic compounds, 5HT agonists, bombesin agonists, galanin antagonists, growth hormone, growth hormone-releasing compounds, TRH agonists, decoupling protein 2 or 3 modulators, leptin agonists, DA agonists (bromocriptine, doprexin), lipase/amylase inhibitors, PPAR modulators, RXR modulators or TR-β agonists.

[0077] In another embodiment of the invention, the further active compound is leptin.

[0078] In another embodiment, the further active compound is dexamphetamine or amphetamine.

[0079] In another embodiment, the further active compound is fenfluramine or dexfenfluramine.

[0080] In another embodiment, the further active compound is sibutramine.

[0081] In another embodiment, the further active compound is orlistat.

[0082] In another embodiment, the further active compound is mazindol or phentermine.

[0083] In another embodiment, the compounds of the formula I are administered in combination with fiber, for instance insoluble fiber, such as, for example, Caromax®. The combination with Caromax® can be administered in a single preparation or by separate administration of compounds of the formula I and Caromax®. Here, Caromax® can also be administered in the form of food, such as, for example, in bakery goods or muesli bars. Compared to the individual active compounds, the combination of compounds of the formula I with Caromax® is, in addition to providing an enhanced action, also characterized by its improved tolerability, for example with respect to the lowering of LDL cholesterol.

[0084] It goes without saying that each suitable combination of the compounds according to the invention with one or more of the compounds mentioned above and optionally one or more further pharmacologically active substances is included in the scope of the present invention.

[0085] The scope of the invention also includes both, stereoisomer mixtures of compounds of the formula I and the pure stereoisomers of the formula I, as well as diastereomer mixtures of the compounds of formula I and the pure diastereomers. The mixtures may be, for example, separated by known chromatographic means.

[0086] One embodiment of the invention includes both, racemic and enantiomerically pure compounds of the formula I of the following structure: 5

[0087] Examples of amino protective groups that can be used include the benzyloxycarbonyl (Z) radical, which can be removed by catalytic hydrogenation; the 2-(3,5-dimethyloxyphenyl)propyl(2)oxycarbonyl(Ddz) or trityl (Trt) radical, which can be removed by weak acids; the t-butylcarbamate (BOC) radical, which can be removed by 3M hydrochloric acid; and the 9-fluorenylmethyloxycarbonyl (Fmoc) radical, which can be removed using secondary amines.

[0088] Another embodiment of the invention relates to a process for preparing diphenylazetidinone derivatives of formula I. 6

[0089] Y can be S, O. (C═O), (C═S), CH═CH, C≡C, N((C1-C6)-alkyl), N(phenyl), N((C1-C6)-alkyl-phenyl), N(CO—(CH2)1-10—COOH) or NH;

[0090] R11 can be H or, if Y=(C═O) or (C═S), then R11 can be OH;

[0091] W, Y′ und W′ can, independently of one another and of Y, be —S(O)m— (wherein m=0-2, —O—), —(C═O)—, —(C═s)—, —CH═CH—, —C≡C—, —N((C1-C6)-alkyl)—, —N(phenyl), —N((C1-C6)-alkyl-phenyl)—, —N(CO—(CH2)1-10—COOH)— or —NH— or a bond;

[0092] x, y and z, independently of one another, can be 0 to 10.

[0093] In compound 11, —(CH2)x—Y—R11 can alternatively also be attached to one of the other two phenyl rings.

[0094] The process for preparing compounds of the formula I comprises reacting, for example, an amine or a hydroxy compound of the formuls II with an alkylating or acylating agent whichmay carry a further functionality (for example in the omega position), if appropriate in protected form. This functionality may be used (after deprotection) for attaching (LAG), for example with the formation of ether, amine or amide bonds.

[0095] The examples below serve to illustrate the invention in more detail, without limiting the invention to the products and embodiments described in the examples.

EXAMPLE I

4-{1-(4-Fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4oxoazetidin-2-yl}-N-hydroxybenzamidine (3):

[0096] 7

[0097] a)4-[5-(4-Fluorophenyl)-1-(4-fluorophenylamino)-5-hydroxy-2-(2-oxo-4-phenyloxazolidine-3-carbonyl)pentyl]benzonitrile (1):

[0098] Under argon, 2.5 g of 3-[5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyloxazolidin-2-one were dissolved in 30 ml of dichloromethane. 3.9 g of 4-[(4-fluorophenylimino)methyl]benzonitrile were added and the mixture was cooled to −10° C. 6.4 ml of diisopropylethylamine and, over a period of 30 min, 4.05 ml of trimethylsilyl chloride were added to this mixture such that the temperature did not exceed −5° C. The mixture was stirred at this temperature for 1 additional hour and then cooled to −25° C. 0.8 ml of titanium tetrachloride was then added slowly. The dark mixture was stirred at from −25° C. to −30° C. over night and then decomposed using 35 ml of a 7% strength solution of tartaric acid. The mixture was stirred for another hour at room temperature. 15 ml of a 20% strength sodium bicarbonate solution were then added, and the mixture was again stirred for 1 hour. After phase separation, the organic phase was washed with 30 ml of water, dried over magnesium sulfate and concentrated to about 10 ml. 2 ml of bistrimethylsilylacetamide were added, and the mixture was then heated to reflux for 30 min and then concentrated under reduced pressure. The residue was cristallized using ethyl acetate/heptane. The product was filtered off with suction and dried under reduced pressure. This gave the product of molecular weight 653.81 (C37H37F2N3O4Si); MS (ESI+): 654.3 (M+H+), 582.2 (M+H+—Si(CH3)3).

[0099] b) {1-(4-Fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-oxoazetidin-2-yl}benzonitrile (2):

[0100] 2 g of 4-[5-(4-fluorophenyl)-1-(fluorophenylamino)-5-hydroxy-2-(2-oxo-4-phenyloxazolidin-3-carbonyl)pentyl]benzonitrile (1) were dissolved in 20 ml of methyl tert-butyl ether and, together with 100 mg of tetrabutylammonium fluoride trihydrate and 1.3 ml of bistrimethylsilylacetamide, heated at 40° C. for about 1 h. The reaction was monitored by thin-layer chromatography. After the reaction ended, initially 0.2 ml of glacial acetic acid was added and the mixture was stirred for 30 min and concentrated. 20 ml of a mixture of isopropanol/2N sulfuric acid=10:1 were added to the residue, and the mixture was stirred for 1 hour. A spatula tip of solid sodium bicarbonate was added and the mixture was then again concentrated under reduced pressure. The residue was taken up in ethyl acetate, the organic phase was washed with water and dried and, after removal of the solvent, the residue was purified by column chromatography (SiO2, CH2Cl2/methanol=100:1). This gave the product of molecular weight 418.45 (C25H20F2N2O2); MS (DCI+): 419 (M+H+).

[0101] c) 4-{1-(4-Fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-oxoazetidin-2-yl}-N-hydroxybenzamidine (3):

[0102] 199 mg of hydroxylammonium hydrochloride were added to a solution of 200 mg of {1-(4-fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-oxoazetidin-2-yl}benzonitrile and 0.45 ml of triethylamine in 15 ml of isopropanol, and the mixture was stirred at room temperature for 12 h. The reaction solution was extracted twice with ethyl acetate/water. The organic phases were dried over magnesium sulfate and concentrated. This gave the product of molecular weight 451.48 (C25H23F2N3O3); MS (ESI): 452.10 (M+H+).

EXAMPLE II

4-{1-(4-Fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-oxoazetidin-2yl}benzamidine (4):

[0103] 8

[0104] 100 mg of 4-{1-(4-fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-oxoazetidin-2-yl}-N-hydroxybenzamidine (3) were dissolved in 100 ml of tetrahydrofuran and, with 2 ml of conc. ammonia, hydrogenated over Raney nickel at a hydrogen pressure of 75 bar and 25° C. for 30 hours. After addition of magnesium sulfate, the reaction solution was filtered. The filtrate was concentrated and separated by HPLC (Knauer Eurospher-100-10-C18, water (0.1% trifluoroacetic acid)/acetonitrile (0.1% trifluoro-acetic acid)=80/20→10/90). This gave the product of molecular weight 435.48 (C25H23F2N3O2); MS (ESI): 436.18 (M+H+).

EXAMPLE III

4-[3-[3-(4-Fluorophenyl)-3-hydroxypropyl]-2-(4-methoxyphenyl)-4-oxoazetidin-1-yl}-N-hydroxybenzamidine (9):

[0105] 9

[0106] a) 3-[5-(tert-Butyidimethylsilanyloxy)-5-(4-fluorophenyl)pentanoyl]-4-phenyl-oxazolidin-2-one (5): 10

[0107] 27 g of 3-[5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyloxazolidin-2-one, 13.6 9 of tert-butyldimethylsilyl chloride and 10.2 g of imidazole were dissolved in 36 ml of dimethylformamide and stirred at 60° C. for 90 min. After the reaction ended, the mixture was dissolved in ethyl acetate and extracted twice with water. The organic phase was dried over magnesium sulfate, filtered and concentrated under reduced pressure. This produced 3-[5-(tert-butyldimethylsilanyloxy)-5-(4-fluorophenyl)pentanoyl]-4-phenyloxazolidin-2-one (5) of molecular weight 471.65 (C26H34FNO4Si); MS (ESI): 340.28 (MH+—HOSi(CH3)2C(CH3)3).

[0108] b) 4-[5-(tert-Butyidimethylsilanyloxy)-5-(4-fluorophenyl)-1-(4-methoxyphenyl)-2-(2-oxo-4-phenyloxazolidine-3carbonyl)pentylamino]benzonitrile (6):

[0109] 16.2 g of 3-[5-(tert-butyidimethylsilanyloxy)-5-(4-fluorophenyl)pentanoyl]-4phenyloxazolidin-2-one were dissolved in 350 ml of dichloromethane. 19.8 ml of Hünig base and 10.14 g of 4-[(4-methoxyphenylimino)methyl]benzonitrile were added to the solution, which was then cooled to −10° C. 8.52 ml of trimethylsilyltriflate were added to the cooled solution, which was then stirred at −10° C. for 30 min. The solution was then cooled to −30° C., and 44 ml of titanium tetrachloride solution were added. The reaction mixture was stirred at a temperature ranging from −30 to −40° C. for 2 h. The solution was then allowed to warm to room temperature and the reaction solution was washed successively with 200 ml of 2N sulfuric acid, 300 ml of 20% strength sodium hydrogen sulfite solution and saturated sodium chloride solution. The organic phase was dried over magnesium sulfate, concentrated under reduced pressure, and the residue was purified on silica gel using n-heptane/ethyl acetate 3/1. This produced 4-[5-(tert-butyldimethylsilanyloxy)-5-(4-fluorophenyl)-1-(4-methoxyphenyl)-2-(2-oxo-4-phenyloxazolidine-3-carbonyl)pentylamino]benzonitrile (6) of molecular weight 707.93 (C41H46FN3O5Si); MS (ESI): 590.51 (MH+-C7H5N2).

[0110] c) 4-[3-[3-(tert-Butyldimethylsilanyloxy)-3-(4-fluorophenyl)propyl]2-(4-methoxyphenyl)-4-oxazetidin-1-yl]benzonitrile (7):

[0111] 13.2 g of 4-[5-(tert-butyldimethylsilanyloxy)-5-(4-fluorophenyl)-1-(4-methoxyphenyl-2-oxo-4-phenyloxazolidine-3-carbonyl)pentylamino]benzonitrile were dissolved in 380 ml of methyl tert-butyl ether. 18.6 ml of N,O-bis(trimethylsilyl)acetamide and 1.86 ml of a 1 M solution of tetrabutylammonium fluoride in tetrahydrofuran were added and the mixture was stirred at room temperature for 2 h. After the reaction ended, 10 ml of acetic acid were added, the reaction mixture was concentrated under reduced pressure and the residue was purified on silica gel using toluene/ethyl acetate 50/1. This produced 4-[3-[3-(tert-butyldimethylsilanyloxy)-3-(4-fluorophenyl)propyl]-2-(4-methoxyphenyl)-4-oxoazetidin-1-yl]benzonitrile (7) of molecular weight 544.75 (C32H37FN2O3Si); MS (ESI): 545.56 (M+H+).

[0112] d) 4-[3-[3-(4-Fluorophenyl)-3-hydroxypropyl]-2-(4-methoxyphenyl)-4-oxazetidin-1-yl]benzonitrile (8):

[0113] 3.5 g of 4-[3-[3-(tert-butyldimethylsilanyloxy)-3-(4-fluorophenyl)propyl]-2-(4-methoxyphenyl)-4-oxoazetidin-1-yl]benzonitrile were dissolved in 65 ml of tetrahydrofuran. 0.74 ml of acetic acid and 8.03 ml of a 1 M solution of tetrabutylammonium fluoride in tetrahydrofuran were added and the mixture was stirred at room temperature for 2 h. Another 4.82 ml of the tetrabutylammonium fluoride solution were then added, and the mixture was stirred at reflux temperature for a further 3 h. The cooled reaction mixture was concentrated under reduced pressure and the residue was purified chromatographically on silica gel using n-heptane/ethyl acetate 2/1. This produced 4-[3-[3-(4-fluorophenyl)-3-hydroxypropyl]-2-(4-methoxyphenyl)-4-oxoazetidin-1-yl]benzontitrile (8) of molecular weight 430.48 (C26H23FN2O3); MS (ESI): 431.24 (M+H+).

[0114] e) 4-[3-[3-(4-Fluorophenyl)-3-hydroxypropyl]-2-(4-methoxyphenyl)-4-oxoazetidin-1-yl]-N-hydroxybenzamidine (9):

[0115] 199 mg of hydroxylammonium hydrochloride were added to a solution of 200 mg of 4-[3-[3-(4-fluorophenyl)-3-hydroxypropyl]-2-(4-methoxyphenyl)-4-oxoazetidin-1-yl]benzonitrile (8) and 0.45 ml of triethylamin in 15 ml of isopropanol. The mixture was stirred at room temperature for 12 h. The reaction solution was extracted twice with ethyl acetate/water. The organic phases were dried over magnesium sulfate and concentrated. This gave the product of molecular weight 463.51 (C26H26F1N3O4); MS (ESI): 464.19 (M+H+).

EXAMPLE IV

4-[3-[3-(4-Fluorophenyl)-3-hydroxypropyl]-2-(4-methoxyphenyl)-4oxoazetidin-1-yl]benzamidine (10):

[0116] 11

[0117] 40 mg of 4-[3-[3-(4-fluorophenyl)-3-hydroxypropyl]-2-(4-methoxyphenyl)-4-oxoazetidin-1-yl]benzamidine were dissolved in 10 ml of tetrahydrofuran and, with 1 ml of conc. ammonia, hydrogenated over Raney nickel at 25° C. for 6.5 h. Magnesium sulfate was added, and the reaction solution was then filtered. The filtrate was concentrated and separated by HPLC (Knauer Eurospher-100-10-C18, water (0.1% trifluoroacetic acid)/acetonitrile (0.1% trifluoroacetic acid)=80/20→10/90). This gave the product of molecular weight 447.51 (C26H26F1N3O3); MS (ESI): 448.20 (M+H+).

EXAMPLE V

4-{2-(4-Fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4oxoazetidin-1-yl}-N-hydroxybenzamidine (12):

[0118] 12

[0119] a) 4-[3-[-(4-Fluorophenyl)-3-hydroxypropyl]-2-(4-fluorophenyl)-4oxoazetidin-1-yl]benzonitrile (11):

[0120] Compound (11) was prepared analogously to the compound of example IIId, the difference being that, instead of 4-[(4-methoxyphenylimino)methyl]benzonitrile, 4-[(4-fluorobenzylidene)amino]benzonitrile was used.

[0121] b) 4-{2-(4-Fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]4oxoazetidin-1-yl}-N-hydroxybenzamidine (12):

[0122] 279 mg of hydroxylammonium hydrochloride were added to a solution of 280 mg of 4-[3-[3-(4-fluorophenyl)-3-hydroxypropyl]-2-(4-fluorophenyl)-4-oxoazetidin-1-yl]benzonitrile (11) and 0.65 ml of triethylamine in 15 ml of isopropanol. The mixture was stirred at room temperature for 12 h. The reaction solution was extracted twice with ethyl acetate/water. The organic phases were dried over magnesium sulfate and concentrated. This gave the product of molecular weight 451.48 (C25H23F2N3O3); (ESI): 452.10 (M+H+).

EXAMPLE VI

4-{2-(4-Fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4oxoazetidin-1-yl}-benzamidine (13):

[0123] 13

[0124] 290 mg of 4-{2-(4-fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-oxoazetidin-1-yl}-N-hydroxybenzamidine (12) were dissolved in 15 ml of tetrahydrofuran and, with 1.5 ml of conc. ammonia, hydrogenated over Raney nickel at 25° C. for 6.5 h. After addition of magnesium sulfate, the reaction solution was filtered. The filtrate was concentrated. This gave the product of molecular weight 435.48 (C25H23F2N3O2); MS (ESI): 436.18 (M+H+).

EXAMPLE VII

1-[5-(4-Fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4oxoazetidin-2-yl}benzylcarbamoyl)pentyl]-4-aza-1-azoniabicyclo[2.2.2]octane trifluoroacetate (15):

[0125] 14

[0126] a) 1-(5-Carboxypentyl)4-aza-1-azonia-bicyclo[2.2.2]octane bromide (14):

[0127] At 70° C., 1.0 g of 6-bromohexanaoic acid in 5 ml of dimethyl sulfoxide was added to a solution of 1.5 g of 1,4-diazabicyclo[2.2.2]octane in 10 ml of dimethylsulfoxide. After 1 h, 100 ml of water were added and the mixture was freeze-dried. The residue was digested with acetone. The residue contained the product of molecular weight 227.33 (cation: C12H23N2O2+); MS (ESI) 227.1 (M+).

[0128] b) 1-[5-(4-{1-[4-Fluorophenyl)-3-[3-(4-fluorophenyl)-3hydroxypropyl]-4-oxoazetidin-2-yl}benzylcarbamoyl)pentyl]-4-aza-1-azoniabicyclo[2.2.2]octane trifluoroacetate (15):

[0129] A solution of 70 mg of 4-(4-aminomethylphenyl)-1-(4-fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]azetidin-2-one and 23 μl of triethylamine in 0.5 ml of dimethylformamide was added to a solution of 76 mg of 1-(5-carboxypentyl)-4-aza-1-azoniabicyclo[2.2.2]octane bromide (14), 64 μl of diisopropylcarbodiimide and 56 mg of hydroxybenzotriazole in 2 ml of dimethylformamide, and the mixture was stirred at room temperature for 12 h. The reaction solution was concentrated and separated by HPLC (Knauer Eurospher-100-10-C18, water (0.1% trifluoroacetic acid)/acetonitrile (0.1% trifluoroacetic acid)=80/20→10/90). This gave the product of molecular weight 631.79 (cation: C37H45F2N403); MS (ESI) 631.34 (M+).

EXAMPLE VIII

1-[5-(4-{3-[3-Hydroxy-3-phenylpropyl)-2-oxo-4-phenylazetidin-1-yl}benzylcarbamoyl)pentyl]-4-aza-1-azoniabicyclo[2.2.2]octane trifluoroacetate (17):

[0130] 15

[0131] a) 1-(4-Aminomethylphenyl)-3-[3-hydroxy-3-phenylpropyl]-4-phenylazetidin-2-one (16):

[0132] Compound (16) was prepared as described in example IIIa-d, the differences being that: a) instead of 4-[(4-methoxyphenylimino)methyl]benzonitrile, 4-(benzylideneamino)benzonitrile was used, b) instead of 3-[5-(4-fluorophenyl)-5-hydroxy-pentanoyl-4-phenyloxazolidin-2-one, 3-[5-(phenyl)-5-hydroxypentanoyl]-4-phenyl-oxazolidin-2-one was used, and c) the product IIId was subjected to reduction with Raney nickel.

[0133] b) 1-[5-(4-{3-[3-Hydroxy-3-phenylpropyl)-2-oxo-4-phenylazetidin-1-yl}benzylcarbamoyl)pentyl]-4-aza-1-azoniabicyclo[2.2.2]octane trifluoroacetate (17):

[0134] The synthesis was carried out analogously to example VIIb starting with 60 mg of 1-(4-aminomethylphenyl)-3-[3-(4-fluorophenyl-3-hydroxypropyl]-4phenylazetidin-2-one. This gave the product of molecular weight 595.81 (cation: C37H47N403); MS (ESI) 595.36 (M+).

EXAMPLE IX

1-[11-(4-{1-(4-Fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-oxoazetidin-2-yl}benzylcarbamoyl)undecyl]-4-aza-1-azoniabicyclo[2.2.2]octane trifluoroacetate (19):

[0135] 16

[0136] a) 1-(11-Carboxyundecyl)-4-aza-1-azoniabicyclo[2.2.2]octane bromide (18):

[0137] The synthesis was carried out analogously to example VIIa starting with 495 mg of 12-bromododecanoic acid. This gave the product of molecular weight 311.49 (cation: C18H35N2O2+); MS (ESI) 311.2 (M+).

[0138] b) 1-[11-(4-{1-(4-Fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-oxoaztidin-2-yl}benzylcarbamoyl)undecyl]-4-aza-1 azoniabicyclo[2.2.2]octane trifluoroacetate (19):

[0139] The synthesis was carried out analogously to example VIIb. This gave the product of molecular weight 715.96 (cation: C43H57F2N4O3); MS (ESI) 715.43 (M+).

EXAMPLE X

1-[11-{4-[3-(3-Hydroxy-3-phenylpropyl)-2-oxo-4-phenylazetidin-1-yl]benzylcarbamoyl}undecyl)-4-aza-1-azoniabicyclo[2.2.2]octane trifluoroacetate (20):

[0140] 17

[0141] The synthesis was carried out analogously to example IXb starting with 74 mg of 1-(4-aminomethylphenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-phenylazetidin-2-one. This gave the product of molecular weight 679.97 (cation: C43H59N4O3); MS (ESI) 679.50 (M+).

EXAMPLE XI

1-[11-{4-[3-[3-(4-Fluorophenyl)-3-hydroxypropyl]-2-(4methoxyphenyl)-4-oxoazetidin-1-yl]benzylcarbamoyl}undecyl)-4-aza-1 azoniabicyclo[2.2.2]octane trifluoroacetate (21):

[0142] 18

[0143] a)

[0144] A solution of 50 mg of 1-(4-aminomethylphenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-4-(4-methoxyphenyl)azetidin-2-one and 25 μl of triethylamine in 1 ml of dimethylformamide was added to a solution of 70 mg of 12-bromododecanoic acid, 50 mg of EDC and 40 mg of hydroxybenzotriazole in 3 ml of dimethylformamide. The mixture was stirred at room temperature for 1 h. The reaction solution was diluted with ethyl acetate and washed three times with aqueous sodium chloride solution. The organic phase was filtered through silica gel, concentrated and separated by flash chromatography. This gave the alkyl bromide (72 mg) with molecular weight 695.72 (C38H48BrFN2O4); MS (ESI) 695.4 (M+H+).

[0145] b)

[0146] 72 mg of the alkyl bromide prepared above and 100 mg of DABCO were stirred in 4 ml of toluene at 100° C. for 20 hours. The reaction solution was concentrated and separated by HPLC (Knauer Eurospher-100-10-C18, water (0.1% trifluoroacetic acid)/acetonitrile (0.1% trifluoroacetic acid)=80/20→10/90). This gave the product of molecular weight 727.99 (cation: C44H60F,N4O4); MS (ESI) 727.5 (M+).

EXAMPLE XII

N-{4-[3-(3-Hydroxy-3-phenylpropyl)-2-oxo-4-phenylazetidin-1 yl]benzyl}-N′, N″-diisopropylguanidine (22)

[0147] 19

[0148] A solution of 76 mg of 1-(4-aminomethylphenyl)-3-[3-hydroxy-3-phenylpropyl]-4-phenylpropyl]-4-phenylazetidin-2-one, 64 μl of diisopropylcarbodiimide, 56 mg of hydroxybenzotriazole and 23 μl triethylamine in 2 ml of dimethylformamide was stirred at room temperature for 22 h. The reaction solution was concentrated and separated by HPLC (Knauer Eurospher-100-10-C18, water (0.1% trifluoroacetic acid)/acetonitrile (0.1% trifluoroacetic acid)=80/20→10/90). This gave the product of molecular weight 512.70 (C32H40N4O2); MS (ESI) 513.4 (M+H+).

EXAMPLE XIII

N-(3-{1-(4-Fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl)-4-oxoazetidin-2-yl}benzyl)-N′,N″-diisopropylguanidine (23)

[0149] 20

[0150] The synthesis was carried out analogously to example XII starting with 60 mg of 4-(4-aminomethylphenyl)-1-(4-fluorophenyl)-3-[3-(4-fluorophenyl)-3-hydroxypropyl]-azetidin-2-one. This gave the product of molecular weight 548.68 (C32H38F2N4O2); MS (ESI) 549.4 (M+H+).

TABLE 1
Compounds of the formula I
21
					Molecular
					weight of
					the free	Molec-
					base or	ular
					acid (cal-	weight
Ex.	R1, R2	R3, R4	R5, R6	Salt	culated)	(found)
XIV	para-O—CH3, H	22	para-F, H	CF3COO−	560.72	561.23 (MH+)
XV	para-O—CH3, H	23	para-F, H	—	547.63	548.33 (MH+)
XVI	24	H, H	para-F, H	I−	473.26	473.3 (M+)
XVII	25	H, H	para-F, H	I−	489.26	489.3 (M+)
XVIII	26	para-F, H	para-F, H	I−	564.27	564.3 (M+)
XIX	para-O—CH3, H	27	para-F, H	I−	576.29	576.3 (M+)
XXI	28	para-F, H	para-F, H	I−	548.27	548.3 (M+)
XXI	para-O—CH3,H	29	para-F, H	I−	560.29	560.3 (M+)
XXII	para-O—CH3, H	30	para-F, H	I−	576.29	576.3 (M+)
XXIII	para-O—CH3, H	31	para-F, H	I−	575.30	575.4 (M+)
XXIV	H, H	32	H, H	I−	527.30	527.3 (M+)
XXV	33	para-F, H	para-F, H	I−	564.27	564.3 (M+)
XXVI	para-O—CH3, H	34	para-F, H	I−	587.30	587.4 (M+)
XXVII	35	para-F, H	para-F, H	I−	575.28	575.3 (M+)
XXVIII	36	para-F, H	para-F, H	CF3COO−	518.26	518.3 (M+)
XXIX	37	H, H	para-F, H	Br−	500.27	500.3 (M+)
XXX	para-O—CH3, H	38	para-F, H	Br−	530.28	530.3 (M+)
XXXI	39	para-F, H	H, H	Br−	500.27	500.3 (M+)
XXXII	40	para-F, H	para-F, H	Br−	574.29	574.3 (M+)
XXXIII	para-O—CH3, H	41	para- F, H	Br−	784.48	784.5 (M+)
XXXIV	42	para-F, H	para-F, H	I−	563.28	563.3 (M+)

[0151] Using the method described below, the activity of the compounds of the formula I according to the invention was examined: ps Effect of the compounds of the invention on cholesterol absorption and 3H-taurocholic acid excretion using fecal excrement of mice, rats or hamsters

[0152] NMRI mice, Wistar rats, or Golden Syrian hamsters (in groups of n=4-6) were kept in metabolic cages, where they were fed with a standard diet (Altromin, Lage (Lippe)). The afternoon prior to the administration of the radioactive tracers (14C-cholesterol), the feed was removed and the animals were adapted to grates.

[0153] Additionally, the animals were labeled s.c. with 3H-TCA (taurocholic acid) (for example 1 μCi/mouse up to 5 μCi/rat) 24 hours prior to the peroral administration of the test meal (14C-cholesterol in Intralipid® 20, Pharmacia-Upjohn).

[0154] Cholesterol absorption test: 0.25 ml/mouse Intralipid ® 20 (Pharmacia-Upjohn) ((spiked with 0.25 μCi of 14C-cholesterol in 0.1 mg of cholesterol) was administered perorally by gavage.

[0155] Test substances were prepared separately in 0.5% methylcellulose (Sigma)/5% Solutol (BASF, Ludwigshafen) or a suitable vehicle.

[0156] The administration volume of the test substance was 0.5 ml/mouse. The test substance was administered immediately prior to the test meal (Intralipid labeled with 14C-cholesterol) (cholesterol absorption test).

[0157] The feces were collected over a period of 24 h: fecal elimination of 14C-cholesterol and 3H-taurocholic acid (TCA) was determined after 24 hours.

[0158] The livers were removed and homogenized, and aliquots were incinerated in an oximate (Model 307, Packard) to determine the amount of 14C-cholesterol which had been taken up/absorbed.

[0159] Evaluation

[0160] Feces Samples

[0161] The total weight was determined, the sample was made up with water to a defined volume and then homogenized, and an aliquot was evaporated to dryness and incinerated in an oximate (Model 307 from Packard for the incineration of radioactively labeled samples). The amount of radioactive 3H—H2O and 14C—CO2 was extrapolated to the amount of 3H-taurocholic acid and 14C-cholesterol, respectively, that was excreted (dual isotope technique). The ED200 values were interpolated from a dose-effect curve as those doses at which the excretion of TCA or cholesterol was doubled, based on a control group treated at the same time.

[0162] Liver Samples

[0163] The amount of 14C-cholesterol taken up by the liver was based on the administered dose. The ED50 values were interpolated from a dose-effect curve as the dose at which the uptake of 14C-cholesterol by the liver was halved (50%), based on a control group.

[0164] The ED50 values below demonstrate the activity of the compounds of the formula I according to the invention

Example No. ED50 (liver) [mg/mouse]
II          0.1
VII         0.1
IX          0.1
X           <1.0
XI          0.3
XV          0.3
XVIII       0.3
XIX         0.3
XXI         0.1
XXII        0.3
XXV         0.1
XXVI        0.03
XXXII       0.3
XXXIV       0.3

[0165] As can be seen from the table, the compounds of the formula I have very good cholesterol-lowering action.

[0166] Bioabsorption:

[0167] The bioabsorption of the compounds of the formula I was examined using the Caco cell model (A. R. Hilgers et al., Caco-2 cell monolayers as a model for drug transport across the intestinal mucosa, Pharm. Res. 1990, 7, 902).

[0168] From the measured data, it can be seen that the bioabsorption of the compounds of the formula I according to the invention was considerably lower than that of the compounds described in the prior art (reference structure): 43

[0169] Reference Structure:

[0170] Ezetimibe

Claims

1. A compound of the formula I,

2. A compound as claimed in claim 1, wherein R2, R4, R5, and R6, independently of one another, are chosen from: H, F, Cl, Br, I, CF3, NO2, N3, CN, COOH, COO(C1-C6)-alkyl, CONH2, CON H(C1-C6)-alkyl, CON[(C1-C6)-alkyl]2, (C1-C6)-alkyl, (C2-C6)-alkenyl, (C2-C6)-alkynyl, and O—(C1-C6)-alkyl, wherein the alkyl radical is unsubstituted or at least one hydrogen in the alkyl radical is replaced by fluorine; or C(═NH)(NH2), PO3H2, SO3H, SO2—NH2, SO2NH(C1-C6)-alkyl, SO2N[(C1-C6)-alkyl]2, S—(C1-C6)-alkyl, S—(CH2)n-phenyl, SO—(C1-C6)-alkyl, SO—(CH2)n-phenyl, SO2—(C1-C6)-alkyl, or SO2—(CH2)n-phenyl, wherein n=0-6, and wherein the phenyl radical is unsubstituted or substituted one or two times, each substituent chosen independently from: F, Cl, Br, OH, CF3, NO2, CN, OCF3, O—(C1-C6)-alkyl, (C1-C6)-alkyl, and NH2; or NH2, NH—(C1-C6)-alkyl, N((C1-C6)-alkyl)2, NH(C1-C7)-acyl, phenyl, or O—(CH2)n-phenyl, wherein n=0-6, and wherein the phenyl ring is unsubstituted or substituted one, two, or three times, each substituent chosen independently from: F, Cl, Br, I, OH, CF3, NO2, CN, OCF3, O—(C1-C6)-alkyl, (C1-C6)-alkyl, NH2, NH(C1-C6)-alkyl, N((C1-C6)-alkyl)2, SO2—CH3, COOH, COO—(C1-C6)-alkyl, and CONH2; R1 and R3, independently of one another, are chosen from: (C0-C30)-alkylene-(LAG); or (C0-C30)-alkylene-(LAG), wherein at least one carbon atom of the alkylene radical is replaced by —O—, —(C═O)—, —N(CH3)— or —NH—, or H, F, Cl, Br, I, CF3, NO2, N3, CN, COOH, COO(C1-C6)-alkyl, CONH2, CONH(C1-C6)-alkyl, CON[(C1-C6)-alkyl]2, (C1-C6)-alkyl, (C2-C6)-alkenyl, (C2-C6)-alkynyl, or O—(C1-C6)-alkyl, wherein the alkyl radical is unsubstituted or at least one hydrogen in the alkyl radical is replaced by fluorine; C(═NH)(NH2), PO3H2, SO3H, SO2—NH2, SO2NH(C1-C6)-alkyl, SO2N[(C1-C6)-alkyl]2, S—(C1-C6)-alkyl, S—(CH2)n-phenyl, SO—(C1-C6)-alkyl, SO—(CH2)n-phenyl, SO2—(C1-C6)-alkyl, or SO2—(CH2)n-phenyl, wherein n=0-6, and wherein the phenyl radical is unsubstituted or substituted one or two times, each substituent chosen independently from: F, Cl, Br, OH, CF3, NO2, CN, OCF3, O—(C1-C6)-alkyl, (C1-C6)-alkyl, and NH2; or NH2, NH—(C1-C6)-alkyl, N((C1-C6)-alkyl)2, NH(C1-C7)-acyl, phenyl, or O—(CH2)n-phenyl, wherein n=0-6, and wherein the phenyl ring is unsubstituted or substituted one, two, or three times, each substituent chosen independently from: F, Cl, Br, I, OH, CF3, NO2, CN, OCF3, O—(C1C6)-alkyl, (C1-C6)-alkyl, NH2, NH(C1-C6)-alkyl, N((C1-C6)-alkyl)2, SO2—CH3, COOH, COO—(C1-C6)-alkyl, and CONH2; wherein (LAG) is a mono-, di- or tricyclic trialkylammonium radical; an acyclic mono-, di- or tricyclic trialkylammoniumalkyl radical; —NR7—C(═NR8)-(NR9R10) (CH2)0-10—C(═NH)(NH2); or —(CH2)0-10—C(═NH)(NHOH); and wherein R7, R8, R9 and R10, independently of one another, are chosen from: H, (C1-C6)-alkyl, (C1-C6)-alkyl-phenyl, phenyl, and (C3-C8)-cycloalkyl; and wherein at least one of the radicals R1 or R3 must have the meaning: (C0-C30)-alkylene-(LAG); or (C0-C30)-alkylene-(LAG), wherein at least one carbon atom of the alkylene radical is replaced by —O—, —(C═O)—, —N(CH3)— or —NH—; or a physiologically acceptablesalt thereof, in any stereoisomeric form, or a mixture of any such compounds in any ratio.

3. A compound as claimed in claim 1, wherein R2, R4, R5, and R6, independently of one another, are chosen from: H, F, Cl, Br, I, CF3, NO2, N3, CN, COOH, COO(C1-C6)-alkyl, CONH2, CONH(C1-C6)-alkyl, CON[(C1-C6)-alkyl]2, (C1-C6)-alkyl, (C2-C6)-alkenyl, (C2-C6)-alkynyl, or O—(C1-C6)-alkyl, wherein the alkyl radical is unsubstituted or at least one hydrogen in the alkyl radical is replaced by fluorine; or C(═NH)(NH2), PO3H2, SO3H, SO2—NH2, SO2NH(C1-C6)-alkyl, SO2N[( C1-C6)-alkyl]2, S—(C1-C6)-alkyl, S—(CH2)n-phenyl, SO—(C1-C6)-alkyl, SO—(CH2)n-phenyl, SO2—(C1-C6)-alkyl, or SO2—(CH2)n-phenyl, wherein n=0-6, and wherein the phenyl radical is unsubstituted or substituted one or two times, each substituent chosen independently from: F, Cl, Br, OH, CF3, NO2, CN, OCF3, O—(C1-C6)-alkyl, (C1-C6)-alkyl, and NH2; or NH2, NH—(C1-C6)-alkyl, N((C1-C6)-alkyl)2, NH(C1-C7)-acyl, phenyl, or O—(CH2)n-phenyl, wherein n=0-6, and wherein the phenyl ring is unsubstituted or substituted one, two, or three times, each substituent chosen independently from: F, Cl, Br, I, OH, CF3, NO2, CN, OCF3, O—(C1-C6)-alkyl, (C1-C6)-alkyl, NH2, NH(C1-C6)-alkyl, N((C1-C6)-alkyl)2, SO2—CH3, COOH, COO—(C1-C6)-alkyl, and CONH2; R1 and R3, independently of one another, are chosen from: —(CH2)0-1—Y—W—(C0-C25)-alkylene-Y′—W′-LAG; or —(CH2)0-1—Y—W—(C0-C25)-alkylene-Y′—W′-LAG, wherein at least one carbon atom of the alkylene radical is replaced by —O—; or H, F, Cl, Br, I, CF3, NO2, N3, CN, COOH, COO(C1-C6)-alkyl, CONH2, CONH(C1-C6)-alkyl, CON[(C1-C6)-alkyl]2, (C1-C6)-alkyl, (C2-C6)-alkenyl, (C2-C6)-alkynyl, or O—(C1-C6)-alkyl, wherein the alkyl radical is unsubstituted or at least one hydrogen in the alkyl radical is replaced by fluorine; or C(═NH)(NH2), PO3H2, SO3H, SO2—NH2, SO2NH(C1-C6)-alkyl, SO2N[(C1-C6)-alkyl]2, S—(C1-C6)-alkyl, S—(CH2)n-phenyl, SO—(C1-C6)-alkyl, SO—(CH2)n-phenyl, SO2—(C1-C6)-alkyl, or SO2—(CH2)n-phenyl, wherein n=0-6, and wherein the phenyl radical is unsubstituted or substituted one or two times, each substituent chosen independently from: F, Cl, Br, OH, CF3, NO2, CN, OCF3, O—(C1-C6)-alkyl, (C1-C6)-alkyl, and NH2; or NH2, NH—(C1-C6)-alkyl, N((C1-C6)-alkyl)2, NH(C1-C7)-acyl, phenyl, or O—(CH2)n-phenyl, wherein n=0-6, and wherein the phenyl ring is unsubstituted or substituted one, two, or three times, each substituent chosen independently from: F, Cl, Br, I, OH, CF3, NO2, CN, OCF3, O—(C1-C6)-alkyl, (C1-C6)-alkyl, NH2, NH(C1-C6)-alkyl, N((C1-C6)-alkyl)2, SO2—CH3, COOH, COO—(Cl-C6)-alkyl, and CONH2; wherein Y, W, Y′ W′, independently of one another, are chosen from: NH, NCH3, C═O, O, a bond, and S(O)m, wherein m=0-2; or Y—W taken together represent a bond; or Y′—W′ taken together represent a bond; and wherein (LAG) is a mono-, di- or tricyclic trialkylammonium radical; a mono-, di- or tricyclic trialkylammoniumalkyl radical; —(CH2)0-10—C(═NH)(NH2); —(CH2)0-10—C(═NH)(NHOH); or —NR7—C(═NR8)(NR9R10); and wherein R7, R8, R9 and R10, independently of one another, are chosen from: H, (C1-C6)-alkyl, (C1-C6)-alkyl-phenyl, phenyl, and (C3-C8)-cycloalkyl, and wherein at least one of the radicals R1 or R3 must have the meaning: —(CH2)0-1—Y—W—(C0-C25)-alkylene-Y′—W′-LAG), wherein one or more carbon atoms of the alkylene radical may be replaced by —O—; or a physiologically acceptable salt thereof, in any stereoisomeric form, or a mixture of any such compounds in any ratio.

4. A compound as claimed in claim 1, wherein LAG is a tricyclic trialkylammoniumalkyl radical; or a pharmaceutically acceptable salt thereof, in any stereoisomeric form, or a mixture of any such compounds in any ratio.

5. A pharmaceutical composition, comprising an effective amount of at least one compound as claimed in claim 1.

6. A pharmaceutical composition, comprising an effective amount of at least one compound as claimed in claim 1 and active compound.

7. The pharmaceutical composition as claimed in claim 6, wherein the at least one additional active compound is chosen from compounds that normalize lipid metabolism.

8. The pharmaceutical composition of claim 6, wherein the at least one additional active compound is chosen from antidiabetics, hypoglycemically active compounds, HMGCoA reductase inhibitors, cholesterol absorption inhibitors, PPAR gamma agonists, PPAR alpha agonists, PPAR alpha/gamma agonists, fibrates, MTP inhibitors, bile acid absorption inhibitors, CETP inhibitors, polymeric bile acid adsorbers, LDL receptor inducers, ACAT inhibitors, antioxidants, lipoprotein lipase inhibitors, ATP citrate lyase inhibitors, squalene synthetase inhibitors, lipoprotein(a) antagonists, lipase inhibitors, insulins, sulfonylureas, biguanides, meglitinides, thiazolidinediones, α-glucosidase inhibitors, active compounds which act on the ATP-dependent potassium channel of the beta cells, CART agonists, NPY agonists, MC4 agonists, orexin agonists, H3 agonists, TNF agonists, CRF agonists, CRF BP antagonists, urocortin agonists, β3 agonists, MSH (melanocyte-stimulating hormone) agonists, CCK agonists, serotonin-reuptake inhibitors, mixed serotonin and noradrenergic compounds, 5HT agonists, bombesin agonists, galanin antagonists, growth hormones, growth hormone-releasing compounds, TRH agonists, decoupling protein 2 or 3 modulators, leptin agonists, DA agonists (bromocriptine, doprexin), lipase/amylase inhibitors, PPAR modulators, RXR modulators or TR-β-agonists and amphetamines.

9. A method for treating impaired lipid metabolism, comprising administering to a patient in need thereof an effective amount of at least one compound as claimed in claim 1.

10. A process for preparing a pharmaceutical composition comprising at least one compound as claimed in claim 1, comprising mixing the at least one compound with a pharmaceutically acceptable carrier and bringing this mixture into a form suitable for administration.

11. A method for treating hyperlipidemia, comprising administering to a patient in need thereof an effective amount of at least one compound as claimed in claim 1.

12. A method for lowering the serum cholesterol concentration, comprising administering to a patient in need thereof an effective amount of at least one compound as claimed in claim 1.

13. A method for treating arteriosclerotic manifestations, comprising administering to a patient in need thereof an effective amount of at least one compound as claimed in claim 1.

14. A method for treating insulin resistance, comprising administering to a patient in need thereof an effective amount of at least one compound as claimed in claim 1.