Patent Application
20190365871
The present invention relates to the use of plasminogen for preventing or treating osteoporosis and its related diseases.
Osteoporosis (OP) is a systemic disease that is characterized by a reduced bone mass and a destructed bone tissue microstructure, and can lead to increased bone fragility and easy fracture. In 2001, the National Institutes of Health (NIH) proposed that osteoporosis is a skeletal system disease characterized by decreased bone strength and an increased risk of fracture. The bone strength reflects two major aspects of bone, i.e. bone mineral density and bone mass. Osteoporosis leads to a reduced bone mass and degeneration of a bone microstructure, which increases the fragility of bones of a patient, thereby severely reducing the motor function and quality of life of the patient.
Mammalian bone development is a highly ordered process that is co-regulated by multiple factors. Mammalian bone development is mainly accomplished by means of two approaches, i.e. intramembranous osteogenesis and endochondral osteogenesis, wherein long bones such as limb bones and vertebrae are mainly formed by means of endochondral osteogenesis, while flat bones such as skull and medial clavicle are formed by means of intramembranous osteogenesis[1]. Bone tissue does not stay invariable after being formed, but is in a dynamic equilibrium of homeostasis between bone formation and absorption. In the process of this dynamic equilibrium, coordinated regulation of hormones, multiple signaling pathways, and bone tissue cells, and homeostasis of mineral salts play an important role[2].
Osteoporosis can be roughly divided into primary and secondary categories, and both postmenopausal osteoporosis and senile osteoporosis belong to primary osteoporosis, and are very common. Secondary osteoporosis is a common systemic bone disease. In addition to known diseases and drugs that induce osteoporosis, some emerging drugs and treatment means have become important causes of secondary osteoporosis.
Epidemiological surveys show that the incidence of osteopenia and osteoporosis in patients with type 1 diabetes mellitus is 48% to 72%[3], and for patients with type 2 diabetes, increased, decreased or no change results in the bone mineral density are all reported in domestic and foreign literatures[4-6]. In recent years, studies have found that the incidence of metabolic bone diseases and the risk of osteoporotic fractures in patients with type 2 diabetes mellitus are significantly higher than those in the general population, and the incidence of osteoporosis in such patients can reach 20% to 60%[5]. Diabetic osteoporosis easily leads to pathological fractures, which causes high disability and mortality, and may aggravate difficulties in the treatment and recovery of diabetic patients.
After more than a century of observation, it has been found that patients with osteoporosis often have a significant increase in mortality due to being complicated with myocardial infarction, stroke, and sudden death; furthermore, patients with atherosclerosis are also often complicated with bone mass loss, leading to occurrence of osteoportic fractures[7-9]. It was believed in the past that atherosclerosis and osteoporosis are degenerative changes arising with increasing age; however, with the long-term clinical observation and the intensive study of the molecular mechanism of the two diseases, it is found that: (1) the two have common risk factors, e.g. ageing, and calcifying vascular cells (CVC) in vascular cells among diabetic vascular cells; in addition, the molecular characteristics and bone biological characteristics are increasingly consistent and parallel, and vascular characteristic indications appear in animals in which bone metabolism-related genes have been knocked out, suggesting that the two diseases have common signaling pathways, transcription factors and interactions of extracellular matrixes; (3) reactive oxygen species (ROS) and oxidized lipids have common influences on blood vessels and bones; (4) endocrine abnormalities such as reduced estrogen, and abnormal metabolisms of parathyroid hormone (PTH), vitamin D and calcitonin occur; and (5) there are also close links between the two diseases in terms of treatment strategies. As the understanding of the mechanism of these two diseases that are seemingly contradictory but often occur in the same organism is deepened, the prevention and treatment of As/OP syndrome are also deepened gradually.
A number of studies in recent years have shown that there is a correlation between a cardiovascular disease and osteoporosis. They both occur at old ages, and are often observed in the same elderly individual, and the incidences of the two diseases increase with age. Although senium is a common risk factor of a cardiovascular disease and osteoporosis, most studies have found that there is still a significant link between the two diseases without considering the age factor. In one aspect, a cardiovascular disease is associated with bone mass loss and an increased risk of fractures, and likewise, there are evidences suggesting that a reduced bone mineral density can lead to an increase in the incidence and mortality of a cardiovascular disease. Further studies have found that a cardiovascular disease and osteoporosis have a close and direct relationship in terms of pathogenesis. Atherosclerosis is the main pathological basis of cardiovascular and cerebrovascular diseases, and arterial calcification is one of its main manifestations. Arterial calcification is considered to be an important marker and a clinical monitoring indicator for a cardiovascular disease. Studies have shown that the essence of vascular calcification is the phenotype transformation of vascular smooth muscle cells into osteoblasts and the transformation of vascular tissues into bone tissues. Furthermore, the formation of vascular calcification is also significantly associated with bone mineral loss. Dr. ZHOU, Rui from the Third Military Medical University[10] conducted an observational study on a group of elderly people at age 60 or more, and discussed the correlation between arterial calcification and osteoporosis and fractures in the elderly patients. His research comprises the following content: 1. From Jan. 1 to Dec. 31, 2012, eligible patients who are at age 60 or more and come into the hospital for visit were screened out as research objects. 2. Semi-quantitative measurement of the degree of aortic calcification: calcified sediments at the abdominal aorta corresponding to the 1st-4th lumbar vertebrae were scored using lumbar spine x-ray lateral radiographs. According to the length of the calcified plaque and the number of affected segments, the aortic calcification score (acs) of each patient ranges from 0 to 24 points, with 0 point for no aortic calcification and 24 points for the most severe aortic calcification. Furthermore, the patients were grouped according to acs. 3. Bone mineral density detection is carried out using a dual energy x-ray absorptiometer (dxa). Osteoporosis is defined as having a bone mineral density value less than that of the bone peak value of the same-sex normal adult of the same race by 2.5 or more standard deviations, as determined based on dxa. 4. The relationship between aortic calcification and the risk of osteoporosis was assessed using a multivariate regression risk model. Furthermore, the research further comprises the following content: 1. Eligible postmenopausal women at age 60 or more were screened out as research objects. 2. Semi-quantitative measurement of the degree of aortic calcification. 3. Diagnosis of vertebral fractures: the morphology of the vertebral body (4th thoracic segment-5th lumbar segment) was observed through an x-ray radiograph to determine the occurrence of vertebral fracture (a thoracic height reduction of 20% or more). 4. The relationship between aortic calcification and vertebral fractures was assessed using a multivariate regression analysis model. Furthermore, the research further comprises the following content: 1. Eligible patients who are at age 60 or more and come into the hospital for visit were screened out as research objects. 2. Bone mineral density testing and diagnosis of osteoporosis. 3. Detection of carotid and coronary atherosclerotic calcified plaques: carotid and coronary cta was carried out using 64-slice spiral ct. All cta images were assessed on a three-dimensional image analysis workstation. Furthermore, the composition and extent of arterial plaques were assessed. 4. The relationship between osteoporosis and bone mass loss and the occurrence risk of carotid and coronary calcified plaques was analyzed using a multivariate regression risk model. It was found from the research results that after adjustment for other confounding factors such as age, severe bone mass loss is significantly associated with the occurrence of carotid plaques, coronary plaques, and coexisting calcified plaques. The conclusion is: severe aortic calcification is associated with the occurrence of osteoporosis in the elderly population. The occurrence risk of osteoporosis increases with increasing atherosclerosis. Decreased bone mineral density and decreased blood 25(OH)D levels are also associated with the occurrence of osteoporosis. In elderly postmenopausal women, severe aortic calcification is associated with the occurrence of vertebral fractures. Decreased bone mineral density and decreased blood 25(OH)D levels are associated with the occurrence of vertebral fractures. In the elderly population, osteoporosis, low bone mass, and decreased blood 25(OH)D levels are associated with the occurrence of arterial calcified plaques; and severe bone mass loss is associated with the occurrence risk of carotid plaques, coronary plaques, and coexisting calcified plaques. The observational study on a group of elderly people in the research revealed some common risk factors for geriatric diseases and the intrinsic interrelationship thereof, which are of great significance for the prevention and treatment of osteoporosis and cardiovascular diseases.
Osteoporosis is one of the representative symptoms of ageing-related conditions and is particularly prevalent in the middle-aged and elderly population. Osteoporosis is a special manifestation of bone ageing in terms of biological ageing, and it has been shown that either too low or too high vitamin D levels are associated with osteoporosis[11]. With the advent of an ageing society, the incidence of osteoporosis is increasing year by year, and the social and economic burden brought about by this is also greatly increasing.
For the treatment of osteoporosis, the key is to restore and maintain normal bone tissue content and reduce the incidence of bone fractures. Although there are many methods of treatment, drug therapies still predominate at present. Commonly used drugs are bone resorption inhibitors, bone formation promoters, and substances for bone mineralization. A bone resorption inhibitor is a drug mainly targeting to osteoclasts, and reduces bone resorption by inhibiting the activity of the osteoclasts; a bone formation promoter is a drug mainly targeting to osteoblasts, and can enhance the activity of the osteoblasts to promote the synthesis of new bones; and a substance for bone mineralization is a basic drug for treating osteoporosis, comprising calcium agents and vitamin D, and can act to supplement bone matrix components. However, most of the drugs currently used for the treatment of osteoporosis are bone resorption inhibitors (such as estrogen, bisphosphonates, and calcitonin), while the types of bone formation promoters (such as parathyroid hormone) are very few. From the perspective of the therapeutic effect of drugs, the situation today only remains at the level of improving symptoms and delaying the development of diseases, but the effect of reversing or even curing the diseases has not been achieved yet; therefore, there is a need to find new therapeutic drugs and treatment methods.
The present invention relates to:
1. A method for preventing and treating osteoporosis and its related conditions, comprising administering a therapeutically effective amount of plasminogen to a subject.
2. The method of item 1, wherein the osteoporosis comprises primary osteoporosis and secondary osteoporosis.
3. The method of item 1, wherein the primary osteoporosis comprises postmenopausal osteoporosis and senile osteoporosis.
4. The method of item 1 or 2, wherein the secondary osteoporosis comprises osteoporosis secondary to an endocrine disease, a rheumatic disease, and a gastrointestinal disease, and osteoporosis caused by a drug therapy.
5. The method of item 4, wherein the secondary osteoporosis comprises osteoporosis caused by a glucocorticoid, primary hyperparathyroidism, hyperthyroidism, primary biliary cirrhosis, hypogonadism, diabetes mellitus, hypertension, atherosclerosis, a chronic kidney disease, rheumatoid arthritis, systemic lupus erythematosus, ankylosing spondylitis, osteoarthritis, a gonadal hormone therapy, an antiepileptic drug therapy, and a chemotherapeutic drug therapy.
6. A method for preventing and treating osteoporosis complicated with a disease, comprising administering an effective amount of plasminogen to a subject, wherein the osteoporosis complicated with a disease comprises osteoporosis complicated with a glucocorticoid therapy, primary hyperparathyroidism, hyperthyroidism, primary biliary cirrhosis, hypogonadism, diabetes mellitus, hypertension, atherosclerosis, a chronic kidney disease, rheumatoid arthritis, systemic lupus erythematosus, ankylosing spondylitis, osteoarthritis, a gonadal hormone therapy, an antiepileptic drug therapy, and a chemotherapeutic drug therapy.
7. A method of preventing an osteoporotic fracture, comprising administering an effective amount of plasminogen to a subject susceptible to osteoporosis, a subject at a high risk of osteoporosis, or a subject diagnosed with osteoporosis to prevent occurrence of a fracture.
8. The method of item 7, wherein the subject comprises a subject receiving a glucocorticoid, or a subject with primary hyperparathyroidism, hyperthyroidism, primary biliary cirrhosis, hypogonadism, diabetes mellitus, hypertension, atherosclerosis, a chronic kidney disease, rheumatoid arthritis, systemic lupus erythematosus, ankylosing spondylitis, or osteoarthritis.
9. The method of item 7, wherein the subject comprises a subject being receiving a gonadal hormone therapy, an antiepileptic drug therapy, or a chemotherapeutic drug therapy.
10. A method for enhancing activity of osteoblasts, comprising administering an effective amount of plasminogen to a subject.
11. A method for regulation of bone mineral metabolism, comprising administering an effective amount of plasminogen to a subject.
12. The method of item 11, wherein the regulation comprises lowering a blood calcium level, increasing a blood phosphorus level, promoting calcium deposition in a bone matrix and/or reducing calcium deposition in a blood vessel wall and an internal organ.
13. The method of any one of items 1 to 12, wherein the plasminogen has at least 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% sequence identity with SEQ ID No. 2, 6, 8, 10 or 12, and still has the plasminogen activity.
14. The method of any one of items 1 to 12, wherein the plasminogen is a protein that has 1-100, 1-90, 1-80, 1-70, 1-60, 1-50, 1-45, 1-40, 1-35, 1-30, 1-25, 1-20, 1-15, 1-10, 1-5, 1-4, 1-3, 1-2 or 1 amino acid added, deleted and/or substituted in SEQ ID No. 2, 6, 8, 10 or 12, and still has the plasminogen activity.
15. The method of any one of items 1 to 12, wherein the plasminogen is a protein that comprises a plasminogen active fragment and still has the plasminogen activity.
16. The method of any one of items 1 to 12, wherein the plasminogen is selected from Glu-plasminogen, Lys-plasminogen, mini-plasminogen, micro-plasminogen, delta-plasminogen or their variants that retain the plasminogen activity.
17. The method of any one of items 1 to 12, wherein the plasminogen is a natural or synthetic human plasminogen, or a variant or fragment thereof that still retains the plasminogen activity.
18. The method of any one of items 1 to 12, wherein the plasminogen is an to ortholog of human plasminogen from a primate or a rodent, or a variant or fragment thereof that still retains the plasminogen activity.
19. The method of any one of items 13 to 18, wherein the amino acids of the plasminogen are as shown in SEQ ID No. 2, 6, 8, 10 or 12.
20. The method of any one of items 1 to 19, wherein the plasminogen is a natural human plasminogen.
21. The method of any one of items 1 to 20, wherein the subject is a human.
22. The method of any one of items 1 to 21, wherein the subject has a lack or deficiency of plasminogen.
23. The method of item 22, wherein the lack or deficiency is congenital, secondary and/or local.
24. A plasminogen for use in the method of any one of items 1 to 23.
25. A pharmaceutical composition, comprising a pharmaceutically acceptable carrier and the plasminogen for use in the method of any one of items 1 to 23.
26. A preventive or therapeutic kit comprising: (i) the plasminogen for use in the method of any one of items 1 to 23, and (ii) a means for delivering the plasminogen to the subject.
27. The kit of item 26, wherein the means is a syringe or a vial.
28. The kit of item 26 or 27, further comprising a label or an instruction for use indicating the administration of the plasminogen to the subject to implement the method of any one of items 1 to 23.
29. An article of manufacture, comprising:
a container comprising a label; and
(i) the plasminogen for use in the method of any one of items 1 to 23 or a pharmaceutical composition comprising the plasminogen, wherein the label indicates the administration of the plasminogen or the composition to the subject to implement the method of any one of items 1 to 23.
30. The kit of any one of items 26 to 28 or the article of manufacture of item 29, further comprising one or more additional means or containers containing additional drugs.
31. The kit or article of manufacture of item 30, wherein the additional drugs comprise drugs for treating osteoporosis, or drugs for treating other diseases complicated with osteoporosis.
32. A medicament for treating osteoporosis comprising plasminogen.
33. A pharmaceutical composition, a kit, or an article of manufacture for treating osteoporosis comprising plasminogen.
34. Use of plasminogen for treating osteoporosis.
35. The use of plasminogen in the preparation of a medicament, a pharmaceutical composition, an article of manufacture, and a kit for use in the method of any one of preceding items 1 to 23.
In any of the above-mentioned embodiments of the present invention, the plasminogen may have at least 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% sequence identity with SEQ ID No. 2, 6, 8, 10 or 12, and still have the activity of plasminogen. In some embodiments, the plasminogen is a protein that has 1-100, 1-90, 1-80, 1-70, 1-60, 1-50, 1-45, 1-40, 1-35, 1-30, 1-25, 1-20, 1-15, 1-10, 1-5, 1-4, 1-3, 1-2 or 1 amino acid added, deleted and/or substituted in SEQ ID No. 2, 6, 8, 10 or 12, and still has the activity of plasminogen.
In some embodiments, the plasminogen is a protein that comprises a plasminogen active fragment and still has the activity of plasminogen. In some embodiments, the plasminogen is selected from Glu-plasminogen, Lys-plasminogen, mini-plasminogen, micro-plasminogen, delta-plasminogen or their variants that retain the plasminogen activity. In some embodiments, the plasminogen is a natural or synthetic human plasminogen, or a variant or fragment thereof that still retains the plasminogen activity. In some embodiments, the plasminogen is an ortholog of human plasminogen from a primate or a rodent, or a variant or fragment thereof that still retains the plasminogen activity. In some embodiments, the amino acids of the plasminogen are as shown in SEQ ID No. 2, 6, 8, 10 or 12. In some embodiments, the plasminogen is a natural human plasminogen.
In some embodiments, the subject is a human. In some embodiments, the subject is lack of or deficient in plasminogen. In some embodiments, the lack or deficiency is congenital, secondary and/or local.
In some embodiments, the pharmaceutical composition comprises a pharmaceutically acceptable carrier and the plasminogen for use in the above-mentioned method. In some embodiments, the kit may be a preventive or therapeutic kit comprising: (i) the plasminogen for use in the above-mentioned method, and (ii) a means for delivering the plasminogen to the subject. In some embodiments, the means is a syringe or a vial. In some embodiments, the kit further comprises a label or an instruction for use indicating the administration of the plasminogen to the subject to implement any one of the above-mentioned methods.
In some embodiments, the article of manufacture comprising: a container comprising a label; and (i) the plasminogen for use in the above-mentioned methods or a pharmaceutical composition comprising the plasminogen, wherein the label indicates the administration of the plasminogen or the composition to the subject to implement any one of the above-mentioned methods.
In some embodiments, the kit or the article of manufacture further comprises one or more additional means or containers containing additional drugs. In some embodiments, the additional drugs are selected from a group of: a hypolipidemic drug, an anti-platelet drug, an antihypertensive drug, a vasodilator, a hypoglycemic drug, an anticoagulant drug, a thrombolytic drug, a hepatoprotective drug, an anti-arrhythmia drug, a cardiotonic drug, a diuretic drug, an anti-infective drug, an antiviral drug, an immunomodulatory drug, an inflammatory regulatory drug and an anti-tumor drug.
In some embodiments of the above-mentioned method, the plasminogen is administered by systemic or topical route, preferably by the following routes: intravenous, intramuscular, and subcutaneous administration of plasminogen for treatment. In some embodiments of the above-mentioned method, the plasminogen is administered in combination with a suitable polypeptide carrier or stabilizer. In some embodiments of the above-mentioned method, the plasminogen is administered at a dosage of 0.0001-2000 mg/kg, 0.001-800 mg/kg, 0.01-600 mg/kg, 0.1-400 mg/kg, 1-200 mg/kg, 1-100 mg/kg or 10-100 mg/kg (by per kg of body weight) or 0.0001-2000 mg/cm2, 0.001-800 mg/cm2, 0.01-600 mg/cm2, 0.1-400 mg/cm2, 1-200 mg/cm2, 1-100 mg/cm2 or 10-100 mg/cm2 (by per square centimeter of body surface area) daily, preferably the dosage is repeated at least once, preferably the dosage is administered at least daily.
The present invention explicitly encompasses all the combinations of technical features belonging to the embodiments of the present invention, and these combined technical solutions have been explicitly disclosed in the present application, as if the above technical solutions were individually and explicitly disclosed. In addition, the present invention also explicitly encompasses all the subcombinations of the various embodiments and elements thereof, and these subcombinations are disclosed as if each of such subcombinations was individually and explicitly disclosed herein.
“Osteoporosis” is a systemic degenerative bone disease characterized by a low bone mass and a damaged bone microstructure, resulting in increased bone fragility and easy fracture. It is generally divided into three categories, primary, secondary and idiopathic osteoporosis.
“Primary osteoporosis” is further divided into postmenopausal osteoporosis (type I) and senile osteoporosis (type II), wherein the postmenopausal osteoporosis usually occurs within 5 to 10 years after menopause in women; and the senile osteoporosis generally refers to osteoporosis in elderly people at age 60 and more. Primary osteoporosis mainly focuses on the important effect of bone mass, bone loss and bone structure, and is clinically characterized by reduced bone mass, increased fragility, structural deterioration and easy fracture.
“Secondary osteoporosis” refers to a disease of reduced bone mass, changed microstructures of bone, and ease of fragility fracture caused by certain diseases, drugs or other reasons. Common diseases or drugs that cause osteoporosis comprise:
1. endocrine diseases:
Cushing's syndrome, hypogonadism, hyperthyroidism, primary hyperparathyroidism, and diabetes mellitus
2. rheumatic diseases:
rheumatoid arthritis, systemic lupus erythematosus, and ankylosing spondylitis
3. blood system diseases:
multiple myeloma, leukemia, lymphoma, thalassemia, and hemophilia
4. drug therapies:
excessive glucocorticoids, excessive thyroid hormone replacement, antiepileptic drugs, lithium or aluminum poisoning, cytotoxic or immunosuppressive agents (cyclosporine A, tacrolimus), heparin, and drugs that cause hypogonadism (aromatase inhibitors, gonadotropin-releasing hormone analogues, etc.)
5. gastrointestinal diseases:
chronic liver diseases (especially primary biliary cirrhosis), inflammatory bowel diseases (especially Crohn's disease), subtotal gastrectomy, and diarrhea
6. kidney diseases:
renal insufficiency or failure
7. hereditary diseases
osteogenesis imperfecta, Marfan's syndrome, hemochromatosis, homocystinuria, and porphyria
8. other reasons:
insufficient vitamin D arising from any reason, alcohol abuse, anorexia nervosa, malnutrition, prolonged bed rest, pregnancy and lactation, chronic obstructive pulmonary disease, cerebrovascular accident, organ transplantation, amyloidosis, multiple sclerosis, and acquired immunodeficiency syndrome
These secondary factors cause osteoporosis by affecting functions of osteoblasts and osteoclasts to result in increased bone resorption and/or reduced bone formation.
The term “secondary osteoporosis” as described in the present invention encompasses osteoporosis caused by various reasons as described above.
“Idiopathic osteoporosis” mainly occurs in adolescents, and generally refers to osteoporosis where the age of onset for male is less than 50 and the age of onset for female is less than 40, without underlying diseases, with the causes being unclear.
“Osteoporosis complicated” with a certain disease or condition refers to osteoporosis that occurs concomitantly with the disease or condition. There may be certain an inherent relationship in terms of etiology or pathogenesis between the disease or condition and osteoporosis. Examples are osteoporosis complicated with diabetes mellitus, osteoporosis complicated with atherosclerosis, osteoporosis complicated with a chronic renal disease, osteoporosis complicated with ankylosing spondylitis, osteoporosis complicated with osteoarthritis, etc.
Plasmin is a key component of the plasminogen activation system (PA system). It is a broad-spectrum protease that can hydrolyze several components of the extracellular matrix (ECM), including fibrin, gelatin, fibronectin, laminin, and proteoglycan[12]. In addition, plasmin can activate some pro-matrix metalloproteinases (pro-MMPs) to form active matrix metalloproteinases (MMPs). Therefore, plasmin is considered to be an important upstream regulator of extracellular proteolysis[13-14]. Plasmin is formed by the proteolysis of plasminogen by two physiological PAs: tissue plasminogen activator (tPA) or urokinase-type plasminogen activator (uPA). Due to the relatively high level of plasminogen in plasma and other body fluids, it is traditionally believed that the regulation of the PA system is primarily achieved through the levels of PA synthesis and activity. The synthesis of PA system components is strictly regulated by different factors, such as hormones, growth factors and cytokines. In addition, there are also specific physiological inhibitors of plasmin and PAs. The main inhibitor of plasmin is α2-antiplasmin. The activity of PAs is simultaneously inhibited by the plasminogen activator inhibitor-1 (PAI-1) of uPA and tPA and regulated by the plasminogen activator inhibitor-2 (PAI-2) that primarily inhibits uPA. There are uPA-specific cell surface receptors (uPARs) that have direct hydrolytic activity on certain cell surfaces[15-16].
Plasminogen is a single-stranded glycoprotein composed of 791 amino acids and has a molecular weight of about 92 kDa[17-18]. Plasminogen is mainly synthesized in the liver and is abundantly present in the extracellular fluid. The content of plasminogen in plasma is about 2 μM. Therefore, plasminogen is a huge potential source of proteolytic activity in tissues and body fluids[19-20]. Plasminogen exists in two molecular forms: glutamic acid-plasminogen (Glu-plasminogen) and lysine-plasminogen (Lys-plasminogen). The naturally secreted and uncleaved forms of plasminogen have an amino-terminal (N-terminal) glutamic acid and are therefore referred to as glutamic acid-plasminogen. However, in the presence of plasmin, glutamic acid-plasminogen is hydrolyzed to lysine-plasminogen at Lys76-Lys77. Compared with glutamic acid-plasminogen, lysine-plasminogen has a higher affinity for fibrin and can be activated by PAs at a higher rate. The Arg560-Val561 peptide bond between these two forms of plasminogen can be cleaved by uPA or tPA, resulting in the formation of plasmin as a disulfide-linked double-strand protease[21]. The amino-terminal portion of plasminogen contains five homotrimeric rings, i.e., the so-called kringles, and the carboxy-terminal portion contains a protease domain. Some kringles contain lysine-binding sites that mediate the specific interaction of plasminogen with fibrin and its inhibitor α2-AP. A newly discovered plasminogen is a 38 kDa fragment, comprising kringles 1-4, is a potent inhibitor of angiogenesis. This fragment is named as angiostatin and can be produced by proteolysis of plasminogen by several proteases.
The main substrate of plasmin is fibrin, and the dissolution of fibrin is the key to prevent pathological thrombosis[22]. Plasmin also has substrate specificity for several components of ECM, including laminin, fibronectin, proteoglycan and gelatin, indicating that plasmin also plays an important role in ECM remodeling[18,23-24]. Indirectly, plasmin can also degrade other components of ECM by converting certain protease precursors into active proteases, including MMP-1, MMP-2, MMP-3 and MMP-9. Therefore, it has been proposed that plasmin may be an important upstream regulator of extracellular proteolysis[25]. In addition, plasmin has the ability to activate certain potential forms of growth factors[26-28]. In vitro, plasmin can also hydrolyze components of the complement system and release chemotactic complement fragments.
“Plasmin” is a very important enzyme that exists in the blood and can hydrolyze fibrin clots into fibrin degradation products and D-dimers.
“Plasminogen” is the zymogenic form of plasmin, and based on the sequence in the swiss prot and calculated from the amino acid sequence (SEQ ID No. 4) of the natural human plasminogen containing a signal peptide, is a glycoprotein composed of 810 amino acids, which has a molecular weight of about 90 kD and is synthesized mainly in the liver and capable of circulating in the blood; and the cDNA sequence encoding this amino acid sequence is as shown in SEQ ID No. 3. Full-length plasminogen contains seven domains: a C-terminal serine protease domain, an N-terminal Pan Apple (PAp) domain and five Kringle domains (Kringles 1-5). Referring to the sequence in the swiss prot, the signal peptide comprises residues Met1-Gly19, PAp comprises residues Glu20-Val98, Kringle 1 comprises residues Cys103-Cys181, Kringle 2 comprises residues Glu184-Cys262, Kringle 3 comprises residues Cys275-Cys352, Kringle 4 comprises residues Cys377-Cys454, and Kringle 5 comprises residues Cys481-Cys560. According to the NCBI data, the serine protease domain comprises residues Val581-Arg804.
Glu-plasminogen is a natural full-length plasminogen and is composed of 791 amino acids (without a signal peptide of 19 amino acids); the cDNA sequence encoding this sequence is as shown in SEQ ID No. 1; and the amino acid sequence is as shown in SEQ ID No. 2. In vivo, Lys-plasminogen, which is formed by hydrolysis of amino acids at positions 76-77 of Glu-plasminogen, is also present, as shown in SEQ ID No.6; and the cDNA sequence encoding this amino acid sequence is as shown in SEQ ID No.5. δ-plasminogen is a fragment of full-length plasminogen that lacks the structure of Kringle 2-Kringle 5 and contains only Kringle 1 and the serine protease domain[29-30]. The amino acid sequence (SEQ ID No. 8) of δ-plasminogen has been reported in the literature[31], and the cDNA sequence encoding this amino acid sequence is as shown in SEQ ID No. 7. Mini-plasminogen is composed of Kringle 5 and the serine protease domain, and has been reported in the literature to comprise residues Val443-Asn791 (with the Glu residue of the Glu-plasminogen sequence that does not contain a signal peptide as the starting amino acid)[31]; the amino acid sequence is as shown in SEQ ID No. 10; and the cDNA sequence encoding this amino acid sequence is as shown in SEQ ID No. 9. Micro-plasminogen comprises only the serine protease domain, the amino acid sequence of which has been reported in the literature to comprise residues Ala543-Asn791 (with the Glu residue of the Glu-plasminogen sequence that does not contain a signal peptide as the starting amino acid)[32], and the sequence of which has been also reported in patent document CN 102154253 A to comprise residues Lys531-Asn791 (with the Glu residue of the Glu-plasminogen sequence that does not contain a signal peptide as the starting amino acid) (the sequence in this patent application refers to the patent document CN 102154253 A); the amino acid sequence is as shown in SEQ ID No. 12; and the cDNA sequence encoding this amino acid sequence is as shown in SEQ ID No. 11.
In the present invention, “plasmin” is used interchangeably with “fibrinolysin” and “fibrinoclase”, and the terms have the same meaning; and “plasminogen” is used interchangeably with “plasminogen” and “fibrinoclase zymogen”, and the terms have the same meaning.
In the present application, the meaning of “lack” in plasminogen is that the content or activity of plasminogen in the body of a subject is lower than that of a normal person, which is low enough to affect the normal physiological function of the subject; and the meaning of “deficiency” in plasminogen is that the content or activity of plasminogen in the body of a subject is significantly lower than that of a normal person, or even the activity or expression is extremely small, and only through exogenous supply can the normal physiological function be maintained.
Those skilled in the art can understand that all the technical solutions of the plasminogen of the present invention are suitable for plasmin. Therefore, the technical solutions described in the present invention cover plasminogen and plasmin.
In the course of circulation, plasminogen is in a closed, inactive conformation, but when bound to thrombi or cell surfaces, it is converted into an active plasmin in an open conformation under the mediation of a plasminogen activator (PA). The active plasmin can further hydrolyze the fibrin clots to fibrin degradation products and D-dimers, thereby dissolving the thrombi. The PAp domain of plasminogen comprises an important determinant that maintains plasminogen in an inactive, closed conformation, and the KR domain is capable of binding to lysine residues present on receptors and substrates. A variety of enzymes that can serve as plasminogen activators are known, including: tissue plasminogen activator (tPA), urokinase plasminogen activator (uPA), kallikrein, coagulation factor XII (Hagmann factor), and the like.
“Plasminogen active fragment” refers to an active fragment in the plasminogen protein that is capable of binding to a target sequence in a substrate and exerting the proteolytic function. The technical solutions of the present invention involving plasminogen encompass technical solutions in which plasminogen is replaced with a plasminogen active fragment. The plasminogen active fragment of the present invention is a protein comprising a serine protease domain of plasminogen. Preferably, the plasminogen active fragment of the present invention comprises SEQ ID No.14, or an amino acid sequence having an amino acid sequence identity of at least 80%, 90%, 95%, 96%, 97%, 98% or 99% with SEQ ID No.14. Therefore, plasminogen of the present invention comprises a protein containing the plasminogen active fragment and still having the plasminogen activity.
At present, methods for determining plasminogen and its activity in blood include: detection of tissue plasminogen activator activity (t-PAA), detection of tissue plasminogen activator antigen (t-PAAg) in plasma, detection of tissue plasminogen activity (plgA) in plasma, detection of tissue plasminogen antigen (plgAg) in plasma, detection of activity of the inhibitor of tissue plasminogen activators in plasma, detection of inhibitor antigens of tissue plasminogen activators in plasma and detection of plasmin-anti-plasmin (PAP) complex in plasma. The most commonly used detection method is the chromogenic substrate method: streptokinase (SK) and a chromogenic substrate are added to a test plasma, the PLG in the test plasma is converted into PLM by the action of SK, PLM acts on the chromogenic substrate, and then it is determined that the increase in absorbance is directly proportional to plasminogen activity using a spectrophotometer. In addition, plasminogen activity in blood can also be determined by immunochemistry, gel electrophoresis, immunonephelometry, radioimmuno-diffusion and the like.
“Orthologues or orthologs” refer to homologs between different species, including both protein homologs and DNA homologs, and are also known as orthologous homologs and vertical homologs. The term specifically refers to proteins or genes that have evolved from the same ancestral gene in different species. The plasminogen of the present invention includes human natural plasminogen, and also includes orthologues or orthologs of plasminogens derived from different species and having plasminogen activity.
“Conservatively substituted variant” refers to one in which a given amino acid residue is changed without altering the overall conformation and function of the protein or enzyme, including, but not limited to, replacing an amino acid in the amino acid sequence of the parent protein by an amino acid with similar properties (such as acidity, alkalinity, hydrophobicity, etc.). Amino acids with similar properties are well known. For example, arginine, histidine and lysine are hydrophilic basic amino acids and are interchangeable. Similarly, isoleucine is a hydrophobic amino acid that can be replaced by leucine, methionine or valine. Therefore, the similarity of two proteins or amino acid sequences with similar functions may be different. For example, the similarity (identity) is 70%-99% based on the MEGALIGN algorithm. “Conservatively substituted variant” also includes a polypeptide or enzyme having amino acid identity of 60% or more, preferably 75% or more, more preferably 85% or more, even more preferably 90% or more as determined by the BLAST or FASTA algorithm, and having the same or substantially similar properties or functions as the natural or parent protein or enzyme.
“Isolated” plasminogen refers to the plasminogen protein that is isolated and/or recovered from its natural environment. In some embodiments, the plasminogen will be purified (1) to a purity of greater than 90%, greater than 95% or greater than 98% (by weight), as determined by the Lowry method, such as more than 99% (by weight); (2) to a degree sufficiently to obtain at least 15 residues of the N-terminal or internal amino acid sequence using a spinning cup sequenator; or (3) to homogeneity, which is determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) under reducing or non-reducing conditions using Coomassie blue or silver staining. Isolated plasminogen also includes plasminogen prepared from recombinant cells by bioengineering techniques and separated by at least one purification step.
The terms “polypeptide”, “peptide” and “protein” are used interchangeably herein and refer to polymeric forms of amino acids of any length, which may include genetically encoded and non-genetically encoded amino acids, chemically or biochemically modified or derivatized amino acids, and polypeptides having modified peptide backbones. The term includes fusion proteins, including, but not limited to, fusion proteins having heterologous amino acid sequences, fusions having heterologous and homologous leader sequences (with or without N-terminal methionine residues); and the like.
The “percent amino acid sequence identity (%)” with respect to the reference polypeptide sequence is defined as the percentage of amino acid residues in the candidate sequence identical to the amino acid residues in the reference polypeptide sequence when a gap is introduced as necessary to achieve maximal percent sequence identity and no conservative substitutions are considered as part of sequence identity. The comparison for purposes of determining percent amino acid sequence identity can be achieved in a variety of ways within the skill in the art, for example using publicly available computer softwares, such as BLAST, BLAST-2, ALIGN or Megalign (DNASTAR) software. Those skilled in the art can determine appropriate parameters for aligning sequences, including any algorithm needed to achieve the maximum comparison over the full length of the sequences being compared. However, for purposes of the present invention, the percent amino acid sequence identity value is generated using the sequence comparison computer program ALIGN-2.
In the case of comparing amino acid sequences using ALIGN-2, the % amino acid sequence identity of a given amino acid sequence A relative to a given amino acid sequence B (or may be expressed as a given amino acid sequence A having or containing a certain % amino acid sequence identity relative to, with or for a given amino acid sequence B) is calculated as follows:
fraction X/Y×100
wherein X is the number of identically matched amino acid residues scored by the sequence alignment program ALIGN-2 in the alignment of A and B using the program, and wherein Y is the total number of amino acid residues in B. It will be appreciated that where the length of amino acid sequence A is not equal to the length of amino acid sequence B, the % amino acid sequence identity of A relative to B will not be equal to the % amino acid sequence identity of B relative to A. Unless specifically stated otherwise, all the % amino acid sequence identity values used herein are obtained using the ALIGN-2 computer program as described in the previous paragraph.
As used herein, the terms “treatment” and “treating” refer to obtaining a desired pharmacological and/or physiologic effect. The effect may be complete or partial prevention of a disease or its symptoms and/or partial or complete cure of the disease and/or its symptoms, and includes: (a) prevention of the disease from developing in a subject that may have a predisposition to the disease but has not been diagnosed as having the disease; (b) suppression of the disease, i.e., blocking its formation; and (c) alleviation of the disease and/or its symptoms, i.e., eliminating the disease and/or its symptoms.
The terms “individual”, “subject” and “patient” are used interchangeably herein and refer to mammals, including, but not limited to, murine (rats and mice), non-human primates, humans, dogs, cats, hoofed animals (e.g., horses, cattle, sheep, pigs, goats) and so on.
“Therapeutically effective amount” or “effective amount” refers to an amount of plasminogen sufficient to achieve the prevention and/or treatment of a disease when administered to a mammal or another subject to treat the disease. The “therapeutically effective amount” will vary depending on the plasminogen used, the severity of the disease and/or its symptoms, as well as the age, body weight of the subject to be treated, and the like.
Plasminogen can be isolated and purified from nature for further therapeutic uses, and can also be synthesized by standard chemical peptide synthesis techniques. When chemically synthesized, a polypeptide can be subjected to liquid or solid phase synthesis. Solid phase polypeptide synthesis (SPPS) is a method suitable for chemical synthesis of plasminogen, in which the C-terminal amino acid of a sequence is attached to an insoluble support, followed by the sequential addition of the remaining amino acids in the sequence. Various forms of SPPS, such as Fmoc and Boc, can be used to synthesize plasminogen. Techniques for solid phase synthesis are described in Barany and Solid-Phase Peptide Synthesis; pp. 3-284 in The Peptides: Analysis, Synthesis, Biology. Vol. 2: Special Methods in Peptide Synthesis, Part A., Merrifield, et al. J. Am. Chem. Soc., 85: 2149-2156 (1963); Stewart et al. Solid Phase Peptide Synthesis, 2nd ed. Pierce Chem. Co., Rockford, Ill. (1984); and Ganesan A. 2006 Mini Rev. Med Chem. 6:3-10 and Camarero J A et al. 2005 Protein Pept Lett. 12:723-8. Briefly, small insoluble porous beads are treated with a functional unit on which a peptide chain is constructed. After repeated cycles of coupling/deprotection, the attached solid phase free N-terminal amine is coupled to a single N-protected amino acid unit. This unit is then deprotected to expose a new N-terminal amine that can be attached to another amino acid. The peptide remains immobilized on the solid phase before it is cut off.
Standard recombinant methods can be used to produce the plasminogen of the present invention. For example, a nucleic acid encoding plasminogen is inserted into an expression vector, so that it is operably linked to a regulatory sequence in the expression vector. Expression regulatory sequence includes, but is not limited to, promoters (e.g., naturally associated or heterologous promoters), signal sequences, enhancer elements and transcription termination sequences. Expression regulation can be a eukaryotic promoter system in a vector that is capable of transforming or transfecting eukaryotic host cells (e.g., COS or CHO cells). Once the vector is incorporated into a suitable host, the host is maintained under conditions suitable for high-level expression of the nucleotide sequence and collection and purification of plasminogen.
A suitable expression vector is usually replicated in a host organism as an episome or as an integral part of the host chromosomal DNA. In general, an expression vector contains a selective marker (e.g., ampicillin resistance, hygromycin resistance, tetracycline resistance, kanamycin resistance or neomycin resistance) to facilitate detection of those exogenous cells transformed with a desired DNA sequence.
Escherichia coli is an example of prokaryotic host cells that can be used to clone a polynucleotide encoding the subject antibody. Other microbial hosts suitable for use include Bacillus, for example, Bacillus subtilis and other species of enterobacteriaceae (such as Salmonella spp. and Serratia spp.), and various Pseudomonas spp. In these prokaryotic hosts, expression vectors can also be generated which will typically contain an expression control sequence (e.g., origin of replication) that is compatible with the host cell. In addition, there will be many well-known promoters, such as the lactose promoter system, the tryptophan (trp) promoter system, the beta-lactamase promoter system or the promoter system from phage lambda. Optionally in the case of manipulation of a gene sequence, a promoter will usually control expression, and has a ribosome binding site sequence and the like to initiate and complete transcription and translation.
Other microorganisms, such as yeast, can also be used for expression. Saccharomyces (e.g., S. cerevisiae) and Pichia are examples of suitable yeast host cells, in which a suitable vector has an expression control sequence (e.g., promoter), an origin of replication, a termination sequence and the like, as required. A typical promoter comprises 3-phosphoglycerate kinase and other glycolytic enzymes. Inducible yeast promoters specifically include promoters derived from alcohol dehydrogenase, isocytochrome C, and enzymes responsible for maltose and galactose utilization.
In addition to microorganisms, mammalian cells (e.g., mammalian cells cultured in cell culture in vitro) can also be used to express and generate the anti-Tau antibody of the present invention (e.g., a polynucleotide encoding a subject anti-Tau antibody). See Winnacker, From Genes to Clones, VCH Publishers, N.Y., N.Y. (1987). Suitable mammalian host cells include CHO cell lines, various Cos cell lines, HeLa cells, myeloma cell lines and transformed B cells or hybridomas. Expression vectors for these cells may comprise an expression control sequence, such as an origin of replication, promoter and enhancer (Queen et al. Immunol. Rev. 89:49 (1986)), as well as necessary processing information sites, such as a ribosome binding site, RNA splice site, polyadenylation site and transcription terminator sequence. Examples of suitable expression control sequences are promoters derived from white immunoglobulin gene, SV40, adenovirus, bovine papilloma virus, cytomegalovirus and the like. See Co et al. J. Immunol. 148:1149 (1992).
Once synthesized (chemically or recombinantly), the plasminogen of the present invention can be purified according to standard procedures in the art, including ammonium sulfate precipitation, affinity column, column chromatography, high performance liquid chromatography (HPLC), gel electrophoresis and the like. The plasminogen is substantially pure, e.g., at least about 80% to 85% pure, at least about 85% to 90% pure, at least about 90% to 95% pure, or 98% to 99% pure or purer, for example free of contaminants such as cell debris, macromolecules other than the subject antibody and the like.
A therapeutic formulation can be prepared by mixing plasminogen of a desired purity with an optional pharmaceutical carrier, excipient or stabilizer (Remington's Pharmaceutical Sciences, 16th edition, Osol, A. ed. (1980)) to form a lyophilized preparation or an aqueous solution. Acceptable carriers, excipients and stabilizers are non-toxic to the recipient at the dosages and concentrations employed, and include buffers, such as phosphates, citrates and other organic acids; antioxidants, including ascorbic acid and methionine; preservatives (e.g., octadecyl dimethyl benzyl ammonium chloride; hexane chloride diamine; benzalkonium chloride and benzethonium chloride; phenol, butanol or benzyl alcohol; alkyl p-hydroxybenzoates, such as methyl or propyl p-hydroxybenzoate; catechol; resorcinol; cyclohexanol; 3-pentanol; and m-cresol); low molecular weight polypeptides (less than about 10 residues); proteins, such as serum albumin, gelatin or immunoglobulins; hydrophilic polymers, such as polyvinylpyrrolidone; amino acids, such as glycine, glutamine, asparagine, histidine, arginine or lysine; monosaccharides, disaccharides and other carbohydrates, including glucose, mannose or dextrins; chelating agents, such as EDTA; sugars, such as sucrose, mannitol, fucose or sorbitol; salt-forming counterions, such as sodium; metal complexes (e.g., zinc-protein complexes); and/or non-ionic surfactants, such as TWEEN′, PLURONICS' or polyethylene glycol (PEG). Preferred lyophilized anti-VEGF antibody formulations are described in WO 97/04801, which is incorporated herein by reference.
The formulations of the invention may also comprise one or more active compounds required for the particular disorder to be treated, preferably those that are complementary in activity and have no side effects with one another, for example anti-hypertensive drugs, anti-arrhythmic drugs, drugs for treating diabetes mellitus, and the like.
The plasminogen of the present invention may be encapsulated in microcapsules prepared by techniques such as coacervation or interfacial polymerization, for example, it may be incorporated in a colloid drug delivery system (e.g., liposomes, albumin microspheres, microemulsions, nanoparticles and nanocapsules), or incorporated in hydroxymethylcellulose or gel-microcapsules and poly-(methyl methacrylate) microcapsules in macroemulsions. These techniques are disclosed in Remington's Pharmaceutical Sciences, 16th edition, Osol, A. Ed. (1980).
The plasminogen of the present invention for in vivo administration must be sterile. This can be easily achieved by filtration through a sterile filtration membrane before or after freeze drying and reconstitution.
The plasminogen of the present invention can be prepared into a sustained-release preparation. Suitable examples of sustained-release preparations include solid hydrophobic polymer semi-permeable matrices having a shape and containing glycoproteins, such as films or microcapsules. Examples of sustained-release matrices include polyesters, hydrogels (e.g., poly(2-hydroxyethyl-methacrylate)) (Langer et al. J. Biomed. Mater. Res., 15: 167-277 (1981); and Langer, Chem. Tech., 12:98-105 (1982)), or poly(vinyl alcohol), polylactides (U.S. Pat. No. 3,773,919, and EP 58,481), copolymer of L-glutamic acid and □ ethyl-L-glutamic acid (Sidman et al. Biopolymers 22:547(1983)), nondegradable ethylene-vinyl acetate (Langer et al. supra), or degradable lactic acid-glycolic acid copolymers such as Lupron Depot™ (injectable microspheres composed of lactic acid-glycolic acid copolymer and leuprolide acetate), and poly D-(−)-3-hydroxybutyric acid. Polymers, such as ethylene-vinyl acetate and lactic acid-glycolic acid, are able to persistently release molecules for 100 days or longer, while some hydrogels release proteins for a shorter period of time. A rational strategy for protein stabilization can be designed based on relevant mechanisms. For example, if the aggregation mechanism is discovered to be formation of an intermolecular S—S bond through thio-disulfide interchange, stability is achieved by modifying sulfhydryl residues, lyophilizing from acidic solutions, controlling moisture content, using appropriate additives, and developing specific polymer matrix compositions.
The pharmaceutical composition of the present invention is administered in different ways, for example by intravenous, intraperitoneal, subcutaneous, intracranial, intrathecal, intraarterial (e.g., via carotid), and intramuscular administration.
Preparations for parenteral administration include sterile aqueous or non-aqueous solutions, suspensions and emulsions. Examples of non-aqueous solvents are propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable organic esters such as ethyl oleate. Aqueous carriers include water, and alcoholic/aqueous solutions, emulsions or suspensions, including saline and buffered media. Parenteral vehicles include sodium chloride solution, Ringer's dextrose, dextrose and sodium chloride, or fixed oils. Intravenous vehicles include liquid and nutrient supplements, electrolyte supplements and the like. Preservatives and other additives may also be present, for example, such as antimicrobial agents, antioxidants, chelating agents and inert gases.
The medical staff will determine the dosage regimen based on various clinical factors. As is well known in the medical field, the dosage of any patient depends on a variety of factors, including the patient's size, body surface area, age, the specific compound to be administered, sex, frequency and route of administration, overall health and other drugs administered simultaneously. The dosage range of the pharmaceutical composition comprising plasminogen of the present invention may be, for example, about 0.0001 to 2000 mg/kg, or about 0.001 to 500 mg/kg (such as 0.02 mg/kg, 0.25 mg/kg, 0.5 mg/kg, 0.75 mg/kg, 10 mg/kg and 50 mg/kg) of the subject's body weight daily. For example, the dosage may be 1 mg/kg body weight or 50 mg/kg body weight, or in the range of 1 mg/kg-50 mg/kg, or at least 1 mg/kg. Dosages above or below this exemplary range are also contemplated, especially considering the above factors. The intermediate dosages in the above range are also included in the scope of the present invention. A subject may be administered with such dosages daily, every other day, weekly or based on any other schedule determined by empirical analysis. An exemplary dosage schedule includes 1-10 mg/kg for consecutive days. During administration of the drug of the present invention, the therapeutic effect and safety are required to be assessed real-timely.
One embodiment of the present invention relates to an article of manufacture or a kit, comprising the plasminogen or plasmin of the present invention useful in the treatment of osteoporosis and its related conditions. The article preferably includes a container, label or package insert. Suitable containers include bottles, vials, syringes and the like. The container can be made of various materials, such as glass or plastic. The container contains a composition that is effective to treat the disease or disorder of the present invention and has a sterile access (for example, the container may be an intravenous solution bag or vial containing a plug that can be pierced by a hypodermic injection needle). At least one active agent in the composition is plasminogen/plasmin. The label on or attached to the container indicates that the composition is used for treating the osteoporosis or its related conditions according to the present invention. The article may further comprise a second container containing a pharmaceutically acceptable buffer, such as phosphate buffered saline, Ringer's solution and glucose solution. It may further comprise other substances required from a commercial and user perspective, including other buffers, diluents, filters, needles and syringes. In addition, the article comprises a package insert with instructions for use, including, for example, instructions to a user of the composition to administer the plasminogen composition and other drugs to treat an accompanying disease to a patient.
One embodiment of the present invention relates to the judgment of treatment efficacy and treatment safety after treating a subject with plasminogen. Common monitoring and assessment contents of therapeutic effect for osteoporosis comprise follow-up survey (adverse reactions, standardized medication, basic measures, re-assessment of fracture risk factors, etc.), new fracture assessment (clinical fracture, body height reduction, and imageological examination), bone mineral density (BMD) measurement, and detection of bone turnover markers (BTM), comprehensive re-assessment based on these data, etc. Among them, BMD and bone mass are currently the most widely used methods for monitoring and assessing the therapeutic effect. For example, BMD can be measured by means of dual energy X-ray absorptiometry (DXA), quantitative computed tomography (QCT), single photon absorption measurement (SPA), or ultrasonometry. BMD can be detected once a year after the start of treatment, and after the BMD has stabilized, the interval may be appropriately extended, for example, to once every 2 years. For BTM, among serological indicators, serum procollagen type 1 N-terminal propeptide (PINP) is relatively frequently used at present as a bone formation indicator, and serum type 1 procollagen C-terminal peptide (serum C-terminal telopeptide, S-CTX) serves as a bone resorption indicator. According to the research progress, more reasonable detection indicators are adjusted where appropriate. Baseline values should be measured prior to the start of treatment, and detections are carried out 3 months after the application of a formation-promoting drug therapy, and 3 to 6 months after the application of a resorption inhibitor drug therapy. BTM can provide dynamic information of bones, is independent of BMD in effect and function, and is also a monitoring means complementary to BMD. The combination of the two has a higher clinical value. In general, if BMD rises or stabilizes after treatment, BTM has an expected change, and no fracture occurs during the treatment, the treatment response can be considered to be good. In addition, the present invention also relates to the judgment of the safety of the therapeutic regimen during and after treating a subject with plasminogen and its variants, including, but not limited to, statistics of the serum half-life, half-life of treatment, median toxic dose (TD50) and median lethal dose (LD50) of the drug in the body of the subject, or observing various adverse events such as sensitization that occur during or after treatment.
In summary, plasminogen can remarkably ameliorate osteoporosis, promote the increase in the bone mineral density and bone mass in various portions of the femur, and the amelioration of the trabecular bone is particularly obvious.
Materials and Methods:
Animals: C57 mice, and Plg+/+ and Plg−/− mice (Jackson Lab) were used for related experiments. The animals were fed in an experimental animal use environment that meets the national standard.
Reagents: vitamin D (Sigma Aldrich, Cat# D1530), corn oil (Sigma Aldrich, Cat# C8267), low calcium special diet (0.2% of calcium, 1% of a phosphate, and 2000 U vitamin D3/kg, from Nantong TROPHIC Feed Technology Co., Ltd., 15 kg), calcium content assay Kit (Nanjing Jiancheng Bioengineering Institute, Cat# C004-2), and human plasminogen (10 mg/ml, purified from healthy plasma donors).
Aloka Micro CT, which is designed exclusively for the observation of mouse and rat morphologies and incorporates the latest third-generation X-ray measurement, is capable of presenting high-quality tomographic images within a short time. It can be used for bone measurement (bone mineral density, bone mineral content, bone volume, bone microstructure, etc.), body fat percentage measurement, visceral and subcutaneous fat identification and measurement, synchronous photography, etc. Bone measurements were carried out on mouse femurs, craniums or lumbar vertebrae as a detection object. After the mice were sacrificed, the femurs, craniums and lumbar vertebrae were taken therefrom and fixed in 4% paraformaldehyde, and the bones were measured using Micro CT (Aloka, manufactured by HITACHI, Japan).
15-week-old wild-type and plasminogen-deficient (Plg−/−) mice, five in each group, were used. Knee joints were taken and fixed in 4% paraformaldehyde for 24 hours, then decalcified in 10% EDTA for three weeks, and washed with a gradient sucrose solution. The above operations need to be carried out at 4° C. The materials were then embedded in paraffin, sectioned into 8 μm and stained with Safranin O. The sections were observed under an optical microscope at 200×.
The results showed that compared with the wild-type mice (
15-week-old wild-type (wt) and plasminogen-deficient (ko) mice, five in each group, were used. Blood was taken from eyeballs removed from the two groups of mice to detect the blood calcium concentration. Under normal conditions, the calcium homeostasis in vivo is very precisely regulated. However, in the case of osteoporosis, calcium loss is a key marker of osteoporosis. In the study of calcium levels in wild-type and Plg−/− mice, we found that the Plg−/− (Ko) mice had significantly higher blood calcium levels at 15 weeks of age than the wild-type mice, and the statistical difference was significant (* indicates P<0.05) (
Eight 20-week-old mice were randomly divided into two groups, i.e. a control group administered with vehicle PBS and a group administered with plasminogen, with 4 mice in each group. On the first day of the experiment, the groups were weighed and grouped, and administered with plasminogen or vehicle PBS. The group administered with plasminogen was injected with plasminogen at a dose of 1 mg/0.1 mL/mouse/day via the tail vein, and the control group administered with vehicle PBS was injected with an equal volume of PBS via the tail vein. The mice were administered consecutively for 30 days and sacrificed on Day 31. The knee joints were taken and fixed in a fixative at 4° C. for 24 hours. The composition of the fixative is: 2% of paraformaldehyde, 0.075 mol/L of lysine, and 0.01 mol/L of sodium periodate. After the fixation, each material was washed with a PBS washing liquid gradient at 4° C. for 12 hours, and then placed in a decalcifying liquid at 4° C. for decalcification for 2 weeks, with the decalcifying liquid being changed every 5 days. After the decalcification was completed, the knee joints were washed with a PBS washing liquid gradient at 4° C. for 12 hours, and were subjected to alcohol gradient dehydration, permeabilization with xylene, paraffin immersion, and paraffin embedding. The sections were 5 μm thick. The sections were dewaxed and rehydrated, stained with hematoxylin and eosin (H&E staining), differentiated with 1% hydrochloric acid in alcohol, and returned to blue with ammonia water. The sections were dehydrated with alcohol gradient, permeabilized with xylene, mounted with a neutral gum, and observed under an optical microscope at 200×.
The results showed that in the control group (
Eight 20-week-old mice were randomly divided into two groups, i.e. a control group administered with vehicle PBS and a group administered with plasminogen, with 4 mice in each group. On the first day of the experiment, the groups were weighed and grouped, and administered with plasminogen or vehicle PBS. The group administered with plasminogen was injected with plasminogen at a dose of 1 mg/0.1 mL/mouse/day via the tail vein, and the control group administered with vehicle PBS was administered with an equal volume of PBS. The mice were administered consecutively for 30 days and sacrificed on Day 31. The femurs were taken and fixed in a fixative at 4° C. for 24 hours. The composition of the fixative is: 2% of paraformaldehyde, 0.075 mol/L of lysine, and 0.01 mol/L of sodium periodate. After the fixation, each material was washed with a PBS washing liquid gradient at 4° C. for 12 hours, and then placed in a decalcifying liquid at 4° C. for decalcification for 2 weeks, with the decalcifying liquid being changed every 5 days. After the decalcification was completed, the knee joints were washed with a PBS washing liquid gradient at 4° C. for 12 hours, and were subjected to alcohol gradient dehydration, permeabilization with xylene, and paraffin embedding. The materials were sectioned into 5 um, deparaffinized, rehydrated, and incubated in a magnesium chloride buffer at 4° C. overnight. The sections were incubated in an alkaline phosphatase substrate solution for 1 hour at room temperature and counterstained with hematoxylin for 2 minutes. The sections were rinsed with running water for 5 minutes, baked at 60° C. for 30 minutes, mounted with a neutral gum, and observed under an optical microscope at 200×.
Alkaline phosphatase (ALP) is a marker of early differentiation of osteoblasts[33]. The results showed that there was only an extremely small amount of alkaline phosphatase staining (indicated by an arrow) on the surface of the articular cartilage in the control group administered with vehicle PBS (
Eight 20-week-old mice were randomly divided into two groups, i.e. a control group administered with vehicle PBS and a group administered with plasminogen, with 4 mice in each group. On the first day of the experiment, the groups were weighed and grouped, and administered with plasminogen or vehicle PBS. The group administered with plasminogen was injected with plasminogen at a dose of 1 mg/0.1 mL/mouse/day via the tail vein, and the control group administered with vehicle PBS was administered with an equal volume of PBS. The mice were administered consecutively for 30 days and sacrificed on Day 31. The femurs were taken and fixed in a fixative at 4° C. for 24 hours. The composition of the fixative is: 2% of paraformaldehyde, 0.075 mol/L of lysine, and 0.01 mol/L of sodium periodate. After the fixation, each material was washed with a PBS washing liquid gradient at 4° C. for 12 hours, and then placed in a decalcifying liquid at 4° C. for decalcification for 2 weeks, with the decalcifying liquid being changed every 5 days. After the decalcification was completed, the femurs were washed with a PBS washing liquid gradient at 4° C. for 12 hours, and were subjected to alcohol gradient dehydration, permeabilization with xylene, and then paraffin embedding. The materials were sectioned into 5 um, deparaffinized, rehydrated, and incubated in a magnesium chloride buffer at 4° C. overnight. The sections were incubated in an alkaline phosphatase substrate solution for 1 hour at room temperature and counterstained with hematoxylin for 2 minutes. The sections were rinsed with running water for 5 minutes, baked at 60° C. for 30 minutes, mounted with a neutral gum, and observed and photographed under a microscope at 200×.
The results showed that in the control group administered with vehicle PBS (
Twenty-five 5- to 6-week-old male C57 mice were taken, weighed and randomly divided into three groups, a blank control group of 5 mice, a group of 10 mice administered with plasminogen, and a control group of 10 mice administered with vehicle PBS. The mice in the blank control group were intraperitoneally injected with 50 μl of corn oil per day; and the mice in the group administered with plasminogen and in the group administered with vehicle PBS were intraperitoneally injected with vitamin D (Sigma Aldrich) at 0.5 μg/kg/day to induce senescence[34,35]. At the same time, the mice were administered in such a manner that the mice in the group administered with plasminogen were injected with human plasminogen at a dose of 1 mg/0.1 mL/mouse/day via the tail vein, the mice in the control group administered with vehicle PBS were injected with an equal volume of PBS via the tail vein, and the mice in the blank control group were not administered. Model establishment and administration were performed continuously for 28 days. During the period of administration, the mice in the blank control group were fed with a low-calcium diet, and the mice in the group administered with plasminogen and in the control group administered with vehicle PBS were fed with a low-calcium diet. The first day of model establishment and administration was set as Day 1. On Day 29, the blood was collected from removed eyeballs and centrifuged to obtain a supernatant to be detected for the activity of serum alkaline phosphatase (ALP).
The results showed that the activity of serum alkaline phosphatase in mice in the group administered with plasminogen was significantly higher than that in mice in the control group administered with vehicle PBS, and the statistical difference was significant; and compared with the control group administered with vehicle PBS, the activity of serum alkaline phosphatase in mice in the group administered with plasminogen was closer to that in the blank control group (
Serum ALP is an isoenzyme glycoprotein, and serum ALP is mainly derived from liver and bone, wherein the ALP derived from bone accounts for 40% to 75%. ALP activity assays are mainly used for diagnosing hepatobiliary and skeletal system diseases. Clinically, in addition to factors such as liver diseases and pregnancy, serum ALP can also reflect the condition of osteogenesis. When bone metabolism is strong, osteoblasts are active, ALP secretion increases, and it is present around osteoblasts and on the surface thereof, is very easily released into the blood, thereby resulting in an increase in the serum ALP activity; therefore, serum ALP is a marker of changes in bone remodeling activity [36]
In this study, the activity of serum alkaline phosphatase in mice in the group administered with plasminogen was significantly higher than that in mice in the control group administered with vehicle PBS, and the statistical difference was significant. This suggests that the plasminogen group can significantly promote the increase in the activity of osteoblasts in vitamin D ageing model mice.
Fifteen 5- to 6-week-old male C57 mice were taken, weighed and randomly divided into three groups, a blank control group, a group administered with plasminogen, and a control group administered with vehicle PBS, with 5 mice in each group. The mice in the blank control group were intraperitoneally injected with 50 μl of corn oil per day; and the mice in the group administered with plasminogen and in the group administered with vehicle PBS were intraperitoneally injected with vitamin D (Sigma Aldrich) at 1 μg/kg/day to induce senescence[34,35]. At the same time, the mice were administered in such a manner that the mice in the group administered with plasminogen were injected with human plasminogen at a dose of 1 mg/0.1 mL/mouse/day via the tail vein, the mice in the control group administered with vehicle PBS were injected with an equal volume of PBS via the tail vein, and the mice in the blank control group were not administered. Model establishment and administration were performed continuously for 28 days. During the period of administration, all the mice were fed with a low-calcium diet (Nantong TROPHIC). The first day of model establishment and administration was set as Day 1. On Day 29, the mice were sacrificed, and knee joints were taken and fixed in a fixative for 24 hours. The composition of the fixative is: 2% of paraformaldehyde, 0.075 mol/L of lysine, and 0.01 mol/L of sodium periodate. After the fixation, each material was washed with a PBS washing liquid gradient at 4° C. for 12 hours, and then placed in a decalcifying liquid at 4° C. for decalcification for 2 weeks, with the decalcifying liquid being changed every 5 days. After the decalcification was completed, the knee joints were washed with a PBS washing liquid gradient at 4° C. for 12 hours, and were subjected to alcohol gradient dehydration, permeabilization with xylene, and paraffin embedding. The materials were sectioned into 5 um, deparaffinized, rehydrated, and incubated in a magnesium chloride buffer at 4° C. overnight. The sections were incubated in an alkaline phosphatase substrate solution for 1 hour at room temperature and counterstained with hematoxylin for 2 minutes. The sections were rinsed with running water for 5 minutes, baked at 60° C. for 30 minutes, mounted with a neutral gum, and observed under an optical microscope at 200×.
The results showed that the alkaline phosphatase positive staining (indicated by an arrow) of the knee joint growth plate in the control group administered with vehicle PBS (
Plg+/+ mice and Plg−/− mice, 12-13, 20-21, and 29-30 weeks old, were taken, 5 in each group, with the body weights of the mice in these groups being substantially the same. The mice were all fed with the same food and water during the experiment. The craniums were taken and fixed in 4% paraformaldehyde for 24 hours, and subjected to Micro CT scanning to determine the bone mineral density.
The results showed that as the week of age increased, the cortical bone mineral density (
Osteoporosis is a systemic skeletal disease that is characterized by a reduced bone mass and a degenerated bone microstructure, and can lead to increased bone fragility and easy fracture. WHO recommends the use of bone mineral density (BMD) measurements to diagnose osteoporosis[37,38]. The above-mentioned experimental results indicate that plasminogen is involved in the regulation of the bone mineral metabolism and plays an important role in a certain period of time.
Plg+/+ mice and Plg−/− mice, 20-21 weeks old, were taken, 5 in each group, with the body weights of these mice being substantially the same. The mice were all fed with the same food and water during the experiment. The craniums were taken and fixed in 4% paraformaldehyde for 24 hours, and subjected to Micro CT scanning to determine the bone mineral content.
The results showed that the bone mineral contents of the cortical bone and total bone in the Plg+/+ mice at 20-21 weeks of age were remarkably higher than those in the Plg−/− mice, and the statistical difference was extremely significant or significant. This indicates that plasminogen plays an important role in the regulation of the bone mineral content of the cranium and is closely related to osteoporosis.
Plg+/+ mice and Plg−/− mice, 12-13, 20-21, and 29-30 weeks old, were taken, 5 in each group, with the body weights of the mice in these groups being substantially the same. The mice were all fed with the same food and water during the experiment. The femurs were taken and fixed in 4% paraformaldehyde for 24 hours, and subjected to Micro CT scanning to determine the bone mineral density.
The results showed that the femoral bone mineral density of Plg+/+ mice increased gradually as the week of age increased during the period of 12-30 weeks of age, while the cortical bone mineral density (
Plg+/+ mice and Plg−/− mice, 12-13, 20-21, and 29-30 weeks old, were taken, 5 in each group, with the body weights of the mice in these groups being substantially the same. The mice were all fed with the same food and water during the experiment. The femurs were taken and fixed in 4% paraformaldehyde for 24 hours, and subjected to Micro CT scanning to determine the bone mineral content.
The results showed that the mineral contents in different portions of the femur of the Plg+/+ mice did not change much or increased gradually as the week of age increased during the period of 12-30 weeks of age, while the mineral contents of the cancellous bone (
Plg+/+ mice and Plg−/− mice, 12-13, 20-21, and 29-30 weeks old, were taken, 5 in each group, with the body weights of the mice in these groups being substantially the same. The mice were all fed with the same food and water during the experiment. The lumbar vertebrae were taken and fixed in 4% paraformaldehyde for 24 hours, and subjected to Micro CT scanning to determine the bone mineral density.
The results showed that the lumbar vertebra bone mineral density of Plg+/+ mice increased gradually as the week of age increased during the period of 12-30 weeks of age, while the cortical bone mineral density (
Plg+/+ mice and Plg−/− mice, 12-13, 20-21, and 29-30 weeks old, were taken, 5 in each group, with the body weights of the mice in these groups being substantially the same. The mice were all fed with the same food and water during the experiment. The lumbar vertebrae were taken and fixed in 4% paraformaldehyde for 24 hours, and subjected to Micro CT scanning to determine the bone mineral content.
The results showed that the mineral content of the lumbar vertebra in the Plg+/+ mice did not change much as the week of age increased during the period of 12-30 weeks of age, while the mineral contents of the cortical bone (
Plg+/+ mice and Plg−/− mice, 12-13, 20-21, and 29-30 weeks old, were taken, 5 in each group, with the body weights of the mice in these groups being substantially the same. The mice were all fed with the same food and water during the experiment. The blood was collected from removed eyeballs from all the mice and centrifuged to obtain a supernatant. The serum alkaline phosphatase activity was measured using an alkaline phosphatase assay kit.
The results showed that the activity of serum alkaline phosphatase in the Plg+/+ mice fluctuated but did not change significantly at 12-30 weeks of age, while the activity of serum alkaline phosphatase in the Plg−/− mice decreased gradually as the week of age increased; the activity of serum alkaline phosphatase in the Plg+/+ mice was significantly higher than that in the Plg−/− mice, and the activities of serum alkaline phosphatase in the two strains of mice showed a remarkable difference at 12 weeks of age; furthermore, as the week of age increased, the difference became more and more significant (
Thirteen 6-week-old male ApoE mice were fed with a high-fat and high-cholesterol diet for 16 weeks to induce atherosclerosis[39,40]. 50 μL of blood was taken from each mouse three days before administration, and the total cholesterol concentration was detected. The mice were randomly divided into two groups based on the detection results, 7 mice in the control group administered with vehicle PBS, and 6 mice in the group administered with plasminogen. The first day of administration was set as Day 1. Mice in the group administered with plasminogen were injected with human plasminogen at a dose of 1 mg/0.1 mL/mouse/day via the tail vein, and mice in the control group administered with vehicle PBS were injected with an equal volume of PBS via the tail vein. During the administration, the mice continued to be fed with the high-fat diet. On Day 30, the mice were fasted for 16 hours. On Day 31, the blood was collected from removed eyeballs, and centrifuged to obtain a supernatant to be detected for the serum calcium concentration. The blood calcium detection was carried out using a calcium detection kit (Nanjing Jiancheng Bioengineering Institute, Cat# C004-2) according to the method in the instructions.
The results showed that the blood calcium concentration in mice in the group administered with plasminogen was remarkably lower than that in the control group administered with vehicle PBS, and the statistical difference was significant (
Thirteen 6-week-old male ApoE mice were fed with a high-fat and high-cholesterol diet (Nantong TROPHIC, TP2031) for 16 weeks to induce the atherosclerosis[39,40] 50 μL of blood was taken from each mouse three days before administration, and the total cholesterol concentration was detected. The mice were randomly divided into two groups based on the detection results, 7 mice in the control group administered with vehicle PBS, and 6 mice in the group administered with plasminogen. The first day of administration was set as Day 1. Mice in the group administered with plasminogen were injected with human plasminogen at a dose of 1 mg/0.1 mL/mouse/day via the tail vein, and mice in the control group administered with vehicle PBS were injected with an equal volume of PBS via the tail vein. During the administration, the mice continued to be fed with the high-fat diet. The mice were sacrificed on the 31st day of administration. The hearts were taken and fixed in 4% paraformaldehyde for 24 to 48 hours, dehydrated and sedimented in 15% and 30% sucrose, and embedded with OCT. They were sectioned into 8 μm thick frozen sections, and stained with alizarin red S for 3 min. The sections were observed under an optical microscope at 40×.
Alizarin red staining can indicate the degree of calcification. The results showed that the calcium deposition in aortic sinus of mice in the group administered with plasminogen (
Nineteen 6-week-old male ApoE mice were fed with a high-fat and high-cholesterol diet (Nantong TROPHIC, TP2031) for 16 weeks to induce atherosclerosis[39,40] 50 μL of blood was taken from each mouse three days before administration, and the total cholesterol concentration was detected. The mice were randomly divided into two groups based on the detection results, 9 mice in the control group administered with vehicle PBS, and 10 mice in the group administered with plasminogen. The first day of administration was set as Day 1. Mice in the group administered with plasminogen were injected with human plasminogen at a dose of 1 mg/0.1 mL/mouse/day via the tail vein, and mice in the control group administered with vehicle PBS were injected with an equal volume of PBS via the tail vein. During the administration, the mice continued to be fed with the high-fat diet. On the 11th day of administration, 5 mice were taken from each group and sacrificed, the femurs were taken therefrom and fixed in 4% paraformaldehyde. On the 31th day of administration, the remaining mice were sacrificed, the femurs were taken therefrom and fixed in 4% paraformaldehyde. The femurs were subjected to Micro CT scanning for determining the bone mineral density.
The correlation between atherosclerosis and osteoporosis has been reported since a long time ago, and hyperlipemia is an important pathogenic factor of atherosclerosis. Recent studies have shown that apolipoprotein E (ApoE) not only affects lipid metabolism, but is also associated with bone mineral density, bone loss, and osteoporotic fractures[41,42].
The results showed that after 10 days of administration, the femoral bone mineral density in mice in the group administered with plasminogen was remarkably higher than that in the control group administered with vehicle PBS, and the statistical difference in terms of cancellous bone mineral density (
Studies have shown that the essence of vascular calcification is the phenotype transformation of vascular smooth muscle cells into osteoblasts and the transformation of vascular tissues into bone tissues. Furthermore, the formation of vascular calcification is also significantly associated with bone mineral loss[10]. From the summary of the experimental results given in Examples 16 and 17 above, it can be seen that plasminogen can enhance bone mineral density while reducing calcium deposition on an arterial wall. It is of great significance for the prevention and treatment of osteoporosis and cardiovascular diseases.
Seven 6-week-old male ApoE mice were fed with a high-fat and high-cholesterol diet (Nantong TROPHIC, TP2031) for 16 weeks to induce atherosclerosis[39,40]. 50 μL of blood was taken from each mouse three days before administration, and the total cholesterol concentration was detected. The mice were randomly divided into two groups based on the detection results, 3 mice in the control group administered with vehicle PBS, and 4 mice in the group administered with plasminogen. The first day of administration was set as Day 1. Mice in the group administered with plasminogen were injected with human plasminogen at a dose of 1 mg/0.1 mL/mouse/day via the tail vein, and mice in the control group administered with vehicle PBS were injected with an equal volume of PBS via the tail vein. During the administration, the mice continued to be fed with the high-fat diet. On the 31th day of administration, the mice were sacrificed, the femurs were taken therefrom and fixed in 4% paraformaldehyde. The materials were then decalcified with an acidic decalcifying liquid (a decalcifying liquid of 8% of hydrochloric acid and 10% of formic acid by volume, prepared in ultrapure water) for 3.5 hours. Then, they were paraffin-embedded, and sectioned into 8 μm for H&E staining, and the sections were observed under an optical microscope at 100× (A and D) and 200× (B, C, E, and F).
The results showed that in the control group administered with vehicle PBS (
Seventeen 8- to 10-week-old C57 female mice were weighed for body weight, and the mice were randomly divided into two groups based on the body weight, a normal control group of 3 mice and a model group of 14 mice. The mice in the model group were anesthetized by means of intraperitoneal injection with pentobarbital sodium at a dose of 50 mg/kg body weight. The hair on both sides of the back of the mice was removed, followed by disinfection with 70% alcohol and iodine. The skin, back muscles and peritoneum were cut open, then the white shiny cellulite was gently pulled out of the incision by means of small forceps, and after the cellulite was separated, the ovaries can be revealed. The fallopian tube at the lower end of an ovary was first ligated with a silk thread, and then the ovary was removed. The incision was sutured, followed by the external application of anti-inflammatory powder. The ovary on the other side was removed by means of the same method. For the normal control mice, they were only cut open at the same position without ovariectomy. 14 days after the ovariectomy, the mice in the model group were randomly divided into two groups based on the body weight, a group administered with plasminogen and a control group administered with vehicle PBS, with 7 mice in each group. The mice in the model group were intraperitoneally injected with dexamethasone at a dose of 125 μg/mouse with an injection frequency of 5 days/week for 12 days in total to induce osteoporosis[43], and the mice in the normal control group were not treated with injection. The mice were administered with drugs while injecting dexamethasone, in such a manner that the mice in the group administered with plasminogen were injected with human plasminogen at a dose of 1 mg/0.1 mL/mouse/day via the tail vein and the mice in the control group administered with vehicle PBS were injected with an equal volume of PBS via the tail vein, both lasting for 16 consecutive days, and the mice in the normal control group were not injected with plasminogen or PBS. The first day of administration was set as Day 1, and on Day 17, the body weights of the mice were measured.
The results showed that the body weight of mice in the control group administered with vehicle PBS was remarkably lighter than that in the normal control group, while the body weight in the group administered with plasminogen was significantly higher than that in the control group administered with vehicle PBS, and the statistical difference was significant (P<0.05) (
Fourteen 8- to 10-week-old C57 female mice were weighed for body weight. All the mice were anesthetized by means of intraperitoneal injection with pentobarbital sodium at a dose of 50 mg/kg body weight. The hair on both sides of the back of the mice was removed, followed by disinfection with 70% alcohol and iodine. The skin, back muscles and peritoneum were cut open, then the white shiny cellulite was gently pulled out of the incision by means of small forceps, and after the cellulite was separated, the ovaries can be revealed. The fallopian tube at the lower end of an ovary was first ligated with a silk thread, and then the ovary was removed. The incision was sutured, followed by the external application of anti-inflammatory powder. The ovary on the other side was removed by means of the same method. 14 days after the ovariectomy, the mice were randomly divided into two groups based on the body weight, a group administered with plasminogen and a control group administered with vehicle PBS, with 7 mice in each group. The mice in the two groups were intraperitoneally injected with dexamethasone at a dose of 125 μg/mouse with an injection frequency of 5 days/week for 12 days in total to induce osteoporosis[43]. The mice were administered with drugs at the same time of model establishment, in such a manner that the mice in the group administered with plasminogen were injected with human plasminogen at a dose of 1 mg/0.1 mL/mouse/day via the tail vein, and the mice in the control group administered with vehicle PBS were injected with an equal volume of PBS via the tail vein, both lasting for 16 consecutive days. The first day of administration was set as Day 1. On Day 17, the mice were sacrificed, and the femurs were taken therefrom and fixed in a 4% paraformaldehyde fixative. Micro CT scanning was carried out for determining various femoral indicators.
The results showed that the volumes (
Seventeen 8- to 10-week-old C57 female mice were weighed for body weight, and the mice were randomly divided into two groups based on the body weight, a normal control group of 3 mice and a model group of 14 mice. The mice in the model group were anesthetized by means of intraperitoneal injection with pentobarbital sodium at a dose of 50 mg/kg body weight. The hair on both sides of the back of the mice was removed, followed by disinfection with 70% alcohol and iodine. The skin, back muscles and peritoneum were cut open, then the white shiny cellulite was gently pulled out of the incision by means of small forceps, and after the cellulite was separated, the ovaries can be revealed. The fallopian tube at the lower end of an ovary was first ligated with a silk thread, and then the ovary was removed. The incision was sutured, followed by the external application of anti-inflammatory powder. The ovary on the other side was removed by means of the same method. For the normal control mice, they were only cut open at the same position without ovariectomy. 14 days after the ovariectomy, the mice in the model group were intraperitoneally injected with dexamethasone at a dose of 125 μg/mouse with an injection frequency of 5 days/week for 12 days in total to induce osteoporosis[43], and the mice in the normal control group were not treated with injection. After the injection with dexamethasone was completed, the mice in the model group were randomly divided into two groups based on the body weight, a group administered with plasminogen and a control group administered with vehicle PBS, with 7 mice in each group. After the model was established (the 2nd day after the dexamethasone injection is completed), the mice were administered with drugs. The mice in the group administered with plasminogen were injected with human plasminogen at a dose of 1 mg/0.1 mL/mouse/day via the tail vein and the mice in the control group administered with vehicle PBS were injected with an equal volume of PBS via the tail vein, both lasting for 16 consecutive days, and the mice in the normal control group were not injected with plasminogen or PBS. The first day of administration was set as Day 1. On Day 17, the mice were sacrificed, and the femurs were taken therefrom and fixed in a 4% paraformaldehyde fixative. The femurs were subjected to Micro CT scanning for determining the femoral bone mineral density.
The results showed that the bone mineral densities of the cortical bone, cancellous bone, trabecular bone and total bone of the femur of mice in the control group administered with vehicle PBS were all smaller than those in the normal control group, while the bone mineral density in each portion of mice in the group administered with plasminogen was greater than that in the control group administered with vehicle PBS. The trend was clear; however, due to the small number of mice, the statistical difference was only close to significant. It can be expected that a statistical difference appears in the case of increasing the number of mice (
The results showed that the bone mineral content in each portion of the femur of mice in the control group administered with vehicle PBS was smaller than that in the normal control group, while the bone mineral content of each portion of mice in the group administered with plasminogen was greater than that in the control group administered with vehicle PBS. The trend was clear; however, due to the small number of mice, the statistical difference was only close to significant. It can be expected that a statistical difference appears in the case of increasing the number of mice (
The results showed that the trabecular bone volume of the femur of mice in the control group administered with vehicle PBS was smaller than that in the normal control group, while the trabecular bone volume of the femur of mice in the group administered with plasminogen was greater than that in the control group administered with vehicle PBS. The trend was clear; however, due to the small number of mice, the statistical difference was only close to significant. It can be expected that a statistical difference appears in the case of increasing the number of mice (
In summary, plasminogen can remarkably ameliorate osteoporosis, promote the increase in the bone mineral density and bone mass in various portions of the femur, and the amelioration of the trabecular bone is particularly obvious.
Fourteen 8- to 10-week-old C57 female mice were weighed for body weight. All the mice were anesthetized by means of intraperitoneal injection with pentobarbital sodium at a dose of 50 mg/kg body weight. The hair on both sides of the back of the mice was removed, followed by disinfection with 70% alcohol and iodine. The skin, back muscles and peritoneum were cut open, then the white shiny cellulite was gently pulled out of the incision by means of small forceps, and after the cellulite was separated, the ovaries can be revealed. The fallopian tube at the lower end of an ovary was first ligated with a silk thread, and then the ovary was removed. The incision was sutured, followed by the external application of anti-inflammatory powder. The ovary on the other side was removed by means of the same method. 14 days after the ovariectomy, the mice were randomly divided into two groups based on the body weight, a group administered with plasminogen and a control group administered with vehicle PBS, with 7 mice in each group. The mice in the two groups were intraperitoneally injected with dexamethasone at a dose of 125 μg/mouse with an injection frequency of 5 days/week for 12 days in total to induce osteoporosis[43]. The mice were administered with drugs at the same time of model establishment, in such a manner that the mice in the group administered with plasminogen were injected with human plasminogen at a dose of 1 mg/0.1 mL/mouse/day via the tail vein, and the mice in the control group administered with vehicle PBS were injected with an equal volume of PBS via the tail vein, both lasting for 16 consecutive days. The first day of administration was set as Day 1. On Day 17, the mice were sacrificed, and the knee joints were taken therefrom and fixed in a 4% paraformaldehyde fixative. The materials were then decalcified with an acidic decalcifying liquid (a decalcifying liquid of 8% of hydrochloric acid and 10% of formic acid by volume, prepared in ultrapure water) for 3.5 hours. Then, they were paraffin-embedded, and sectioned into 3 μm for H&E staining (A and B) and Safrain 0 staining (C and D), and the sections were observed under an optical microscope at 100×.
The results showed that in the groups administered with vehicle PBS (
Fourteen 8- to 10-week-old C57 female mice were weighed for body weight. All the mice were anesthetized by means of intraperitoneal injection with pentobarbital sodium at a dose of 50 mg/kg body weight. The hair on both sides of the back of the mice was removed, followed by disinfection with 70% alcohol and iodine. The skin, back muscles and peritoneum were cut open, then the white shiny cellulite was gently pulled out of the incision by means of small forceps, and after the cellulite was separated, the ovaries can be revealed. The fallopian tube at the lower end of an ovary was first ligated with a silk thread, and then the ovary was removed. The incision was sutured, followed by the external application of anti-inflammatory powder. The ovary on the other side was removed by means of the same method. 14 days after the ovariectomy, the mice were randomly divided into two groups based on the body weight, a group administered with plasminogen and a control group administered with vehicle PBS, with 7 mice in each group. The mice in the two groups were intraperitoneally injected with dexamethasone at a dose of 125 μg/mouse with an injection frequency of 5 days/week for 12 days in total to induce osteoporosis[43]. The mice were administered with drugs at the same time of model establishment, in such a manner that the mice in the group administered with plasminogen were injected with human plasminogen at a dose of 1 mg/0.1 mL/mouse/day via the tail vein, and the mice in the control group administered with vehicle PBS were injected with an equal volume of PBS via the tail vein, both lasting for 16 consecutive days. The first day of administration was set as Day 1. On Day 17, the mice were sacrificed, and knee joints were taken therefrom and fixed in a fixative. The composition of the fixative is: 2% of paraformaldehyde, 0.075 mol/L of lysine, and 0.01 mol/L of sodium periodate. After the fixation, each material was washed with a PBS washing liquid gradient at 4° C. for 12 hours, and then placed in a decalcifying liquid at 4° C. for decalcification for 2 weeks, with the decalcifying liquid being changed every 5 days. After the decalcification was completed, the knee joints were washed with a PBS washing liquid gradient at 4° C. for 12 hours, and were subjected to alcohol gradient dehydration, permeabilization with xylene, and paraffin embedding. The materials were sectioned into 3 um, deparaffinized, rehydrated, and incubated in a magnesium chloride buffer at 4° C. overnight. The sections were incubated in an alkaline phosphatase substrate solution for 1 hour at room temperature and counterstained with hematoxylin for 2 minutes. The sections were rinsed with running water for 5 minutes, baked at 60° C. for 30 minutes, mounted with a neutral gum, and observed under an optical microscope at 200×.
The results showed that the alkaline phosphatase staining of the knee joint cartilage tissue (indicated by thin arrow) and the growth plate (indicated by thick arrow) of mice in the control groups administered with vehicle PBS (
Eleven 8- to 10-week-old Plg+/+ female mice were used. The mice were anesthetized by means of intraperitoneal injection with pentobarbital sodium at a dose of 50 mg/kg body weight. The hair on both sides of the back of the mice was removed, followed by disinfection with 70% alcohol and iodine. The skin, back muscles and peritoneum were cut open, then the white shiny cellulite was gently pulled out of the incision by means of small forceps, and after the cellulite was separated, the ovaries can be revealed. The fallopian tube at the lower end of an ovary was first ligated with a silk thread, and then the ovary was removed. The incision was sutured, followed by the external application of anti-inflammatory powder. The ovary on the other side was removed by means of the same method[44,45]. 65 days after the ovariectomy, the mice were weighed and randomly divided into two groups based on the body weight, a group of 6 mice administered with plasminogen and a control group of 5 mice administered with vehicle PBS, and administration was started. The mice in the group administered with plasminogen were injected with human plasminogen at a dose of 1 mg/0.1 mL/mouse/day via the tail vein, and the mice in the control group administered with vehicle PBS were injected with an equal volume of PBS via the tail vein, both lasting for 11 consecutive days. The first day of administration was set as Day 1. On Day 12, the blood was collected from removed eyeballs, and centrifuged to obtain a supernatant to be detected for the blood calcium concentration. The blood calcium detection was carried out using a calcium detection kit (Nanjing Jiancheng Bioengineering Institute, Cat# C004-2) according to the method in the instructions.
The results showed that the serum calcium concentration in mice in the group administered with plasminogen was remarkably lower than that in the control group administered with vehicle PBS, and the statistical difference was significant (* indicates P<0.05) (
Eleven 8- to 10-week-old Plg+/+ female mice were used. The mice were anesthetized by means of intraperitoneal injection with pentobarbital sodium at a dose of 50 mg/kg body weight. The hair on both sides of the back of the mice was removed, followed by disinfection with 70% alcohol and iodine. The skin, back muscles and peritoneum were cut open, then the white shiny cellulite was gently pulled out of the incision by means of small forceps, and after the cellulite was separated, the ovaries can be revealed. The fallopian tube at the lower end of an ovary was first ligated with a silk thread, and then the ovary was removed. The incision was sutured, followed by the external application of anti-inflammatory powder. The ovary on the other side was removed by means of the same method[44,45]. 65 days after the ovariectomy, the mice were weighed and randomly divided into two groups based on the body weight, a group of 6 mice administered with plasminogen and a control group of 5 mice administered with vehicle PBS, and administration was started. The mice in the group administered with plasminogen were injected with human plasminogen at a dose of 1 mg/0.1 mL/mouse/day via the tail vein, and the mice in the control group administered with vehicle PBS were injected with an equal volume of PBS via the tail vein, both lasting for 11 consecutive days. The first day of administration was set as Day 1. On Day 12, the blood was collected from removed eyeballs, and centrifuged to obtain a supernatant to be detected for the blood phosphorus concentration. The blood phosphorus detection was carried out using a phosphorus detection kit (Nanjing Jiancheng Bioengineering Institute, Cat# C006-3) according to the method in the instructions.
The results showed that the serum phosphorus concentration in mice in the group administered with plasminogen was remarkably higher than that in the control group administered with vehicle PBS, and the statistical difference was significant (* indicates P<0.05) (
Sixteen 9-week-old male C57 mice were fed with a 3% cholesterol high-fat diet (Nantong TROPHIC) for 4 weeks to induce hyperlipemia[46,47]. This model was designated as the 3% cholesterol hyperlipemia model. The model mice continued to be fed with a 3% cholesterol high-fat diet. 50 μL of blood was taken from each mouse three days before administration, and the total cholesterol was detected. The mice were randomly divided into two groups based on the total cholesterol concentration and the body weight, 8 mice in each group. The first day of administration was recorded as Day 1. Mice in the group administered with plasminogen were injected with human plasminogen at a dose of 1 mg/0.1 mL/mouse/day via the tail vein, and an equal volume of PBS was administered to mice in the control group administered with vehicle PBS via the tail vein, both lasting for 20 days. On Day 20, the mice were fasted for 16 hours; and on Day 21, the mice were sacrificed, and the knee joints were taken therefrom and fixed in a fixative. The composition of the fixative is: 2% of paraformaldehyde, 0.075 mol/L of lysine, and 0.01 mol/L of sodium periodate. After the fixation, each material was washed with a PBS washing liquid gradient at 4° C. for 12 hours, and then placed in a decalcifying liquid at 4° C. for decalcification for 2 weeks, with the decalcifying liquid being changed every 5 days. After the decalcification was completed, the knee joints were washed with a PBS washing liquid gradient at 4° C. for 12 hours, and were subjected to alcohol gradient dehydration, permeabilization with xylene, and paraffin embedding. The materials were sectioned into 3 um, deparaffinized, rehydrated, and incubated in a magnesium chloride buffer at 4° C. overnight. The sections were incubated in an alkaline phosphatase substrate solution for 1 hour at room temperature and counterstained with hematoxylin for 2 minutes. The sections were rinsed with running water for 5 minutes, baked at 60° C. for 30 minutes, mounted with a neutral gum, and observed under an optical microscope at 200×
Hyperlipemia is a lipid metabolism disorder that can cause a series of complications. In recent years, a number of studies have found that hyperlipemia is a common cause of osteoporosis and atherosclerosis[48,49].
The results showed that the alkaline phosphatase staining (indicated by an arrow) of the knee joint of mice in the groups administered with plasminogen (
Fourteen 8- to 10-week-old C57 female mice were weighed for body weight. The mice were anesthetized by means of intraperitoneal injection with pentobarbital sodium at a dose of 50 mg/kg body weight. The hair on both sides of the back of the mice was removed, followed by disinfection with 70% alcohol and iodine. The skin, back muscles and peritoneum were cut open, then the white shiny cellulite was gently pulled out of the incision by means of small forceps, and after the cellulite was separated, the ovaries can be revealed. The fallopian tube at the lower end of an ovary was first ligated with a silk thread, and then the ovary was removed. The incision was sutured, followed by the external application of anti-inflammatory powder. The ovary on the other side was removed by means of the same method. 14 days after the ovariectomy, the mice in the model group were intraperitoneally injected with dexamethasone at a dose of 125 μg/mouse with an injection frequency of 5 days/week for 12 days in total to induce osteoporosis[43]. After the injection with dexamethasone was completed, the mice were randomly divided into two groups based on the body weight, a group administered with plasminogen and a control group administered with vehicle PBS, with 7 mice in each group. After the model was established (the 2nd day after the dexamethasone injection is completed), the mice were administered with drugs. The mice in the group administered with plasminogen were injected with human plasminogen at a dose of 1 mg/0.1 mL/mouse/day via the tail vein, and the mice in the control group administered with vehicle PBS were injected with an equal volume of PBS via the tail vein, both lasting for 16 consecutive days. The first day of administration was set as Day 1. On Day 17, the mice were sacrificed, and the knee joints were taken therefrom and fixed in a 4% paraformaldehyde fixative. The materials were then decalcified with an acidic decalcifying liquid (a decalcifying liquid of 8% of hydrochloric acid and 10% of formic acid by volume, prepared in ultrapure water) for 3.5 hours. Then, they were paraffin-embedded, and sectioned into 3 μm for H&E staining (A and B) and Safrain 0 staining (C and D), and the sections were observed under an optical microscope at 100×
The results showed that in the groups administered with vehicle PBS (
| Number | Date | Country | Kind |
|---|---|---|---|
| PCT/CN2016/110163 | Dec 2016 | CN | national |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/CN2017/116592 | 12/15/2017 | WO | 00 |
