Patent Application
20230258566
The invention relates to a fluorimeter reference material and its use in calibration of spectrofluorimetry instruments.
Wavelength calibration of monochromator based spectrofluorometers is traditionally achieved through measuring the position of the atomic lines of the excitation lamp or a noble gas/mercury pen light in the sample position.
When calibrating the wavelength accuracy of the excitation monochromator the position of the main atomic line of the Xenon excitation source at 467.3 nm may be measured. This is a single wavelength calibration; this method does not validate the entire spectrum of the monochromator. Validation in this context means that the measurements provide wide-ranging and documented evidence that the instrument is functioning precisely and is distinct from calibration.
When calibrating the wavelength accuracy of the emission monochromator, the atomic lines of a calibrated light source in the form of a mercury, Krypton or Neon pen lamp may be used. These calibrated light sources provide emission lines across the visible and NIR spectrum but are generally relatively expensive, require frequent calibration and the use of a diffuse reflector setup.
Some instrument manufacturers therefore recommend the use of a well-characterised reference solution such as Holmium oxide in perchloric acid typically used for wavelength accuracy validation in absorbance spectroscopy, coupled with a reflector. The reflector is placed in the sample position and the holmium solution is placed in the path of the excitation beam. A synchronous scan with Δλ=0 nm (i.e. the excitation and emission monochromators are set to the same position and moved at the same rate across the spectrum) or either the excitation or emission monochromator is set to zero order (i.e. the monochromator acts as a mirror and reflects all wavelengths of light) and the other monochromator is scanned across the spectrum.
This creates a projection of the holmium absorbance spectrum which is reflected off the surface of the reflector and into the detector.
This method works well with any optically transparent chemical solution or material which has sharply defined and/or well characterised absorbance spectra and allows determination of the accuracy of both the excitation and emission monochromators simultaneously.
A reflector or light scatterer is required in the sample position because a fluorescence spectrometer will have the detector oriented perpendicular or otherwise adjacent to the light source, rather than opposite the sample compartment as in transmission spectroscopy.
Many solutions can be provided in traceable/certified format, are mentioned in various pharmacopeia standards and can therefore be used to provide wavelength validation across the UV, visible and Near-IR spectrum. The main downsides to this methodology is that both a reference solution and a reflector are required as well as generally requiring a bespoke holder for each instrument type in order to keep the solution cell in the correct position in the excitation beam.
An alternative methodology places a layer of wavelength accuracy standard between a fluorescent material that displays a broad band of fluorescence when excited over a particular wavelength range and the detector. The fluorescence emission is attenuated by the absorption properties of wavelength accuracy standard. This method only validates the emission monochromator and can only be used over a range where the absorption of the standard and the emission of the fluorescent material overlap.
Examples of these approaches can be seen in US2005287040A1 and US2010243876A1
The known methods are relatively time consuming to perform and require a certain expertise to undertake accurately.
The present invention therefore seeks to provide new means for calibrating a fluorimeter that are easier and more convenient to use.
According to the invention there is provided a fluorimeter reference cell comprising a first material, which first material emits an absorption spectrum when an excitation light is incident upon the first material, which absorption spectrum has a defined characteristic enabling wavelength calibration of the fluorimeter, wherein the reference cell further comprises a second material, which second material has dispersive properties such that the absorption spectrum is scattered or reflected at an angle to the angle of incidence of the excitation light incident on the reference cell.
Preferred aspects of the invention can be found in the sub-claims.
The invention advantageously provides an integrated, convenient, single part reference cell for utilising a projected image of the well-defined and characterised absorbance spectra of a rare-earths or other solution typically used for wavelength accuracy validation in absorbance spectroscopy.
The invention advantageously provides a single reference unit which can fit into the standard cuvette holder of a conventional spectrofluorometer which comprises both the wavelength accuracy reference solution and a reflector in a single part.
Exemplary embodiments of the invention will now be described in greater detail with reference to the drawings in which:
In use, incident excitation light is absorbed at specific wavelengths by the solution and a projection of the absorbance spectrum is scattered by the suspended colloid particles into the detector which is typically located at 90° or otherwise adjacent to the incident excitation light.
This customised cell is then filled with a solution 21 typically used for wavelength accuracy validation in absorbance spectroscopy such as holmium oxide in perchloric acid. The cell is then permanently sealed.
Excitation light energy enters the cell through one of the adjacent sides of the cell and is absorbed at specific wavelengths by the solution. A projection of the absorbance spectrum is scattered or reflected by the coating/finish of the modified face into the instrument detector, usually located at 90° to the incident light.
Excitation light energy enters the block through one of the adjacent faces and is absorbed at specific wavelengths by the glass. A projection of the absorbance spectrum is scattered or reflected by the coating/finish of the modified face into the instrument detector.
The embodiments of the invention are useful as a wavelength validation and/or calibration standard in any scanning spectrofluorometer capable of collecting synchronous scans or setting monochromators to zero order. The above embodiments can be used as supplied, without the need for additional adaptors or holders in the prior art. They are further suitable for scanning spectrofluorometers that measure at a right-angle or front-face sample geometry
Certification measurements defining the wavelength positions of the absorption peaks of the solution can be generated by a reference spectrophotometer. The excitation and/or emission monochromators of a scanning spectrofluorometer may be considered out of calibration if the wavelength positions of the peaks in the transmission spectrum are not at the same position as the certified values given in the certificate for the reference solution.
In addition to the specifically described holmium oxide solutions, alternatives having well defined absorption spectra both as glasses or solutions suitable for wavelength calibration in this manner include, but are not limited to:
The wavelength accuracy solution/glass does not exhibit fluorescence of its own and the measured spectra is purely a reflection of the excitation beam attenuated at certain wavelengths by the solution/glass.
Although the invention has been specifically described in relation to a spectrofluorometer, it would be applicable in other types of fluorimeter such as a filter fluorimeter.
| Number | Date | Country | Kind |
|---|---|---|---|
| 2014108.1 | Sep 2020 | GB | national |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/EP2021/074737 | 9/8/2021 | WO |
