Larval Transcription Factor Mediating Juvenile Hormone Suppression of Genes

Information

• NSF Award
• 9318247

Owner

• UNIVERSITY OF KENTUCKY, A CORP. OF KY

• Award Id

  9318247

• Award Effective Date

  8/15/1994 - 31 years ago

• Award Expiration Date

  7/31/1999 - 26 years ago

• Award Amount

  $ 349,786.00

• Award Instrument

  Continuing grant

9318247 Jones This research is aimed at determining the molecular mechanisms underlying the phenomenon of development of a larval caterpillar form into the winged, adult moth. The maintenance of a larval form, and the suppression of adult form, is due to the maintenance of a high titer of juvenile hormone during the larval stage. The molecular mechanism by which a high titer of juvenile hormone causes suppression of genes associated with the adult form is not well understood. It is the objective of the present grant research to determine the mechanisms, using as a model system the suppression of expression of hexamerin genes by juvenile hormone. In this proposed research, we will analyze the regulatory promoter DNA associated with the hexamerin genes for the presence of proteins that bind to the DNA in a JH- sensitive manner. By various tests, including gel mobility shift analysis, DNA footprinting and cell transfection assay with wild type and mutant sequences, we will narrow down to and identify the particular DNA bases to which the regulatory proteins bind. ***

• Program Officer

  Judith A. Verbeke

• Min Amd Letter Date

  8/10/1994 - 31 years ago

• Max Amd Letter Date

  3/27/1998 - 27 years ago

• ARRA Amount

• Name

  University of Kentucky

• City

  Lexington

• State

  KY

• Country

  United States

• Address

  201 Kinkead Hall

• Postal Code

  405060001

• Phone Number

  8592579420

• First Name

  Grace

• Last Name

  Jones

• Email Address

  gjones@uky.edu

• Start Date

  8/15/1994 12:00:00 AM

• Name

  Other Applications NEC

• Code

  99

• Name

  Life Science Biological

• Code

  61

• Text

  INTEGRATIVE ANIMAL BIOLOGY

• Code

  1155

• Text

  UNDERGRADUATE EDUCATION

• Code

  9178

• Text

  GENERAL FOUNDATIONS OF BIOTECHNOLOGY

• Code

  9183

• Text

  RES EXPER FOR UNDERGRAD-SUPPLT

• Code

  9251

• Text

  BIOTECHNOLOGY

• Text

  SCIENCE, MATH, ENG & TECH EDUCATION