Claims
- 1. A transgenic plant resistant to the effects of externally imposed stresses, wherein the transgenic plant comprises a nucleotide sequence comprising an exogenous tonoplast pyrophosphate driven H+ pump gene operably linked to a promoter.
- 2. The transgenic plant of claim 1, wherein the externally imposed stresses to which the plant is resistant are selected from the group consisting of drought, prolonged exposure to temperatures below 0° C., and a growth medium high in salt content.
- 3. The transgenic plant of claim 2, wherein the growth medium is selected form the group consisting of soil and water.
- 4. The transgenic plant of claim 1, wherein the exogenous tonoplast driven H+ pump gene encodes AVP1, or a homolog thereof.
- 5. The method of claim 4, wherein the AVP1, or homolog thereof, is encoded by a gene present in a construct designed to overexpress AVP1, or homolog thereof.
- 6. The method of claim 4, wherein the construct comprises the AVP1 gene, or homologue thereof, operably linked to a chimeric promoter designed to overexpress AVP1.
- 7. The method of claim 4, wherein the AVP1 gene or homologue thereof is operably linked to a chimeric promoter selected from the group consisting of tissue specific promoters, constitutive promoters, inducible promoters and combinations thereof.
- 8. The method of claim 4, wherein the AVP1 gene is operably linked to a tissue-specific promoter that promotes expression of AVP1 in pollen.
- 9. The method of claim 4, wherein the AVP1 gene, or homolog thereof, is operably linked to a double tandem enhancer of a 35S CaMV promoter.
- 10. The method of claim 4, wherein the AVP1 gene, or homolog thereof, is derived from a wild type plant.
- 11. The method of claim 4, wherein the AVP1, or homolog thereof, is derived from a transgenic plant.
- 12. A seed produced by the transgenic plant of claim 1.
- 13. A progeny plant from the seed of claim 12.
- 14. A transgenic plant obtained by introducing into the genome of the plant exogenous nucleic acid that alters expression of vacuolar pyrophosphatase in the transgenic plant.
- 15. Plant cells comprising exogenous nucleic acid that alters expression of vacuolar pyrophosphatase in the plant cell.
- 16. The plant cells of claim 15, wherein the cells are selected from the group consisting of root cells and stem cells.
- 17. The plant cells of claim 15, wherein the exogenous nucleic acid encodes AVP1.
- 18. The plant cells of claim 17, wherein the AVP1 is derived from a wild type plant of the same species from which the transgenic plant is derived.
- 19. The plant cells of claim 17, wherein the AVP1 is derived from a wild type plant of a different species from which the transgenic plant is derived.
- 20. A method for increasing production of seeds in plants comprising the steps of:
(a) providing pollen from a first plant, wherein said first plant has been transformed with a tonoplast pyrophosphate driven H+ pump gene operably linked to a promoter to create a transgenic plant; (b) fertilizing a second plant of the same species from which the first plant is derived with the pollen from the transgenic plant; and (c) culturing the fertilized plant until the plant produces mature seeds.
- 21. The method of claim 20, wherein the tonoplast pyrophosphatase driven H+ pump gene transformed into the first plant is exogenous.
- 22. The method of claim 20, wherein the second plant is a transgenic plant.
- 23. The method of claim 20, wherein the second plant is a wild type plant.
- 24. The method of claim 21, wherein said exogenous tonoplast pyrophosphate driven H+ pump gene is operably linked to a chimeric promoter.
- 25. The method of claim 24, wherein said exogenous tonoplast pyrophosphate driven H+ pump gene encodes AVP1.
- 26. A plant seed produced by the method of claim 21.
- 27. A progeny plant grown from the plant seed of claim 26.
- 28. The method of claim 22, wherein the first and second plants are from the species A. thaliana.
- 29. The method of claim 22, wherein the first and second plants are from the species Nicotinia tabacum.
- 30. The method of claim 22, wherein the second plant has been transformed with a polynucleotide sequence comprising an exogenous tonoplast pyrophosphatase driven H+ pump gene operably linked to a promoter.
- 31. A plant seed produced by the method of claim 22.
- 32. A progeny plant grown from the plant seed of claim 31.
REFERENCE TO RELATED APPLICATIONS
[0001] This application claims benefit, under Title 35, U.S.C. § 119(e), of U. S. application Ser. No. 60/226,223, filed Aug. 18, 2000.
Provisional Applications (1)
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Number |
Date |
Country |
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60226223 |
Aug 2000 |
US |