NOVEL CHEMILUMINESCENT DETECTION OF GENE REARRANGEMENTS

Information

  • Research Project
  • 2423585
  • ApplicationId
    2423585
  • Core Project Number
    R43CA075830
  • Full Project Number
    1R43CA075830-01
  • Serial Number
    75830
  • FOA Number
  • Sub Project Id
  • Project Start Date
    9/1/1997 - 27 years ago
  • Project End Date
    6/30/1998 - 26 years ago
  • Program Officer Name
  • Budget Start Date
    9/1/1997 - 27 years ago
  • Budget End Date
    6/30/1998 - 26 years ago
  • Fiscal Year
    1997
  • Support Year
    1
  • Suffix
  • Award Notice Date
    8/21/1997 - 27 years ago
Organizations

NOVEL CHEMILUMINESCENT DETECTION OF GENE REARRANGEMENTS

The objective of Phase I is to develop simple, sensitive and unambiguous chemiluminescent detection of juxtaposed genes in chromosomal translocations associated with many cancers. We propose chemiluminescent assay formats based on simultaneous probing and detection of both genes of a translocation in a single Southern blot. Phase I will prove the feasibility by developing a Southern hybridization assay for the simultaneous detection of the juxtaposed bcl-2 oncogene and the JH DNA segment of the immunoglobulin heavy chain locus in the t(14;18) translocations. Simultaneous detection will be achieved using two types of chemiluminescent formats: a dual substrate that emits light only by the combined action of two different antibody-enzyme conjugates bound to the target DNA in close proximity to each other; and consecutive application of separate chemiluminescent substrates where the chemiluminescence generated from one probe in the first step is completely stopped in the second step when the second enzyme initiates light from the other probe. The proposed methods can be adapted to the detection of many different juxtaposed genes associated with cancers. The methods developed in Phase I will be used to Phase II to develop kits for rapid microtiter plate assays for gene rearrangements. PROPOSED COMMERCIAL APPLICATION: The proposed chemiluminescent assays should provide the basis for a new method and kits for detection of various gene arrangements resulting from chromosomal translocations associated with specific types of cancer. The proposed assays will be simpler and more rapid than current methods of detecting gene rearrangements and will permit unambiguous detection in a single test. The detection system can also be used in developing kits for developing minimal residual disease during cancer therapies.

IC Name
NATIONAL CANCER INSTITUTE
  • Activity
    R43
  • Administering IC
    CA
  • Application Type
    1
  • Direct Cost Amount
  • Indirect Cost Amount
  • Total Cost
  • Sub Project Total Cost
  • ARRA Funded
  • CFDA Code
    396
  • Ed Inst. Type
  • Funding ICs
  • Funding Mechanism
  • Study Section
    ZRG2
  • Study Section Name
  • Organization Name
    LUMIGEN, INC.
  • Organization Department
  • Organization DUNS
    193979002
  • Organization City
    SOUTHFIELD
  • Organization State
    MI
  • Organization Country
    UNITED STATES
  • Organization Zip Code
    480344302
  • Organization District
    UNITED STATES