Claims
- 1. A filtrate-holder plate comprising polypropylene and Teflon® and containing 96 wells, wherein:
each of said wells is capable of securely containing a Microcon-3, 10, 30 or 100® filter unit; said wells contain an opening on their bottom surface sufficient in size to allow the base of said filter unit, but not the reservoir or filter membrane of said filter unit, to pass through the bottom surface and into a reversible-well collection plate; said wells have a circular circumference; said wells are arranged in 8 rows of twelve wells each in the plate; the center of each well is aligned vertically with the wells in the rows above and below said well and the center of each well is aligned horizontally with the center of the wells in columns to the left and right of said well; and wherein:
the exterior bottom surface of said-filtrate holder plate contains a plurality of pegs protruding therefrom sufficient in number and size to connect said plate to a reversible-well collection plate; the side and bottom surfaces of said filtrate-holder plate contain a plurality of V-shaped grooves sufficient in number and size to allow the plate by a standard automated device; the external dimensions of said filtrate-holder plate are about the same as the external dimensions of a standard 96-well plate.
- 2. The plate of claim 1, wherein the plate is composed of from about 1-10% Teflon® and from about 80-99% polypropylene.
- 3. The plate of claim 2, wherein the plate is composed of about 2.5% Teflon® and about 97.5% polypropylene.
- 4. The filtrate holder of claim 1, wherein the filter unit is a Micron-10® filter unit.
- 5. A filtrate-holder plate comprising polypropylene and Teflon® and containing 96 wells as shown in FIGS. 4, 5, 7 and 8.
- 6. A reversible-well collection plate comprising polypropylene and Teflon® and containing 96 wells, wherein:
each of said wells is capable of securely containing a Microcon-3, 10, 30 or 100® filter unit; the top of said well is capable of securely containing either end of the filter unit; wherein the wells have a U-shaped bottom surface sufficient to allow said surface to securely contain either end of said filter unit; and wherein:
the side and bottom surfaces of said reversible-well collection plate contain a plurality of V-shaped grooves sufficient in number and size to allow the plate by a standard automated device; the external dimensions of said reversible-well collection plate are about the same as the external dimensions of a standard 96-well plate.
- 7. The plate of claim 6, which is composed of from about 1-10% Teflon® and from about 80-99% polypropylene.
- 8. The plate of claim 7, which is composed of about 2.5% Teflon® and about 97.5% polypropylene.
- 9. The plate of claim 6, wherein the filter unit is a Micron-10® filter unit.
- 10. A reversible-well collection plate comprising polypropylene and Teflon® and containing 96 wells as shown in FIGS. 9, 10, 11 and 12.
- 11. A high-throughput screening assay for the detection of the binding of small molecules to protein which comprises the steps of:
(a) loading a sample of liquid containing the small molecule into a Microcon-3, 10, 30 or 100® filter unit having a membrane to which protein is bound; (b) placing said loaded filter unit of step (a) securely into the filtrate-holder plate of claim 1;(c) securely attaching the bottom of said loaded filtrate-holder plate of step (b) to the top of the reversible-well collection plate of claim 6, such that the base of said filter unit protrudes through the bottom of the filtrate-holder plate and fits securely into the wells of the reversible-well collection plate; (d) collecting the filtrate from the wells of the collection plate so as to determine the small molecules which did notd (“free”) to the protein, wherein said collection is by centrifuging the plate combination of step (c) for a period of time sufficient to allow the free small molecules to pass through the filter membrane and into the collection wells, while not allowing substantially any of the bound small molecules to pass through the membrane; and, (e) collecting the bound molecules from step (d), wherein said collecting is by connecting the wells of a second reversible-well collection plate, said wells facing down, securely to the top ends of the filter units, securely inverting the filter unit/reversible-well plate ation and then centrifuging the inverted combination for a period of time sufficient to allow the bound small molecules to pass into the wells of the collection plate.
Parent Case Info
[0001] This application claims the benefit of the filing dates of U.S. Provisional Application Serial Nos. 60/127,624 and 60/145,527.
Provisional Applications (2)
|
Number |
Date |
Country |
|
60127624 |
Apr 1999 |
US |
|
60145527 |
Jul 1999 |
US |
Divisions (1)
|
Number |
Date |
Country |
Parent |
09540030 |
Mar 2000 |
US |
Child |
10304934 |
Nov 2002 |
US |