SENSORS FOR IMAGING KINASE ACTIVITY IN LIVE CELLS

Information

  • Research Project
  • 6227279
  • ApplicationId
    6227279
  • Core Project Number
    R43CA079380
  • Full Project Number
    3R43CA079380-01S1
  • Serial Number
    79380
  • FOA Number
  • Sub Project Id
  • Project Start Date
    9/28/1998 - 26 years ago
  • Project End Date
    3/27/2000 - 24 years ago
  • Program Officer Name
    STRASBURGER, JENNIFER
  • Budget Start Date
    9/28/1998 - 26 years ago
  • Budget End Date
    3/27/2000 - 24 years ago
  • Fiscal Year
    2000
  • Support Year
    1
  • Suffix
    S1
  • Award Notice Date
    2/11/2000 - 24 years ago
Organizations

SENSORS FOR IMAGING KINASE ACTIVITY IN LIVE CELLS

Kinases are a family of important signal-transducing enzymes that are activated when molecules bind to cell-surface receptors. Kinases phosphorylate cytoplasmic proteins, amplifying the binding event. There are currently no commercially available methods for imaging intracellular kinase activity. Imaging kinase activation in live cells could be useful for screening libraries of drug candidates for their ability to initiate signal transduction. To address this problem, the proposed Phase I research intends to investigate the use of reversible molecular sensors for imaging kinase activity in live cells. fluorescent, peptidic substrates for kinase will be synthesized and tested for response to phosphorylation in vitro and in vivo. The goal will be to design visible-wavelength, reversible kinase sensors useful for imaging the enzyme-mediated flux of phosphorylation. Initial enzyme targets will be protein kinase C (PKC), casein kinase Il (CklI) and tyrosine kinase. The result will be a method similar to that used for the imaging the flux of intracellular Ca2+ and will be applicable to imaging kinase activity in both single cells and populations of cells. Reversible kinase sensors could offer important insights into signal transduction pathways and also serve as powerful tools for high throughput screening and drug discovery. PROPOSED COMMERCIAL APPLICATIONS: Reagents proposed herein will result in assays for high-throughput screening, sensors for imaging kinase activity in live cells and methods for the detection of kinase activity in vitro.

IC Name
NATIONAL CANCER INSTITUTE
  • Activity
    R43
  • Administering IC
    CA
  • Application Type
    3
  • Direct Cost Amount
  • Indirect Cost Amount
  • Total Cost
    44860
  • Sub Project Total Cost
  • ARRA Funded
  • CFDA Code
    396
  • Ed Inst. Type
  • Funding ICs
    NCI:44860\
  • Funding Mechanism
  • Study Section
    ZRG3
  • Study Section Name
    Special Emphasis Panel
  • Organization Name
    HELIX RESEARCH COMPANY
  • Organization Department
  • Organization DUNS
  • Organization City
    GOSHEN
  • Organization State
    OR
  • Organization Country
    UNITED STATES
  • Organization Zip Code
    97405
  • Organization District
    UNITED STATES