Patent Application
20240002313
The present invention relates to a [11C]-labeled acyclic retinoid, a central nervous system activator, and methods for producing them. Since the [11C]-labeled acyclic retinoid of the present invention has intracerebral migration, it can be suitably used as a PET probe for studying intracerebral kinetics of acyclic retinoids. Further, the central nervous system activator of the present invention contains an acyclic retinoid, which is an unlabeled body of the [11C]-labeled acyclic retinoid of the present invention, and has an effect of migrating into a brain and activating a central nervous system. Therefore, it can be used as a therapeutic agent for central nervous system degenerative diseases and a lead compound for development thereof. Here, the “central nervous system degenerative disease” refers to all diseases caused by degeneration of a central nerve (for example, Alzheimer-type dementia, frontotemporal dementia, Lewy body dementia, Parkinson's disease, progressive supranuclear palsy, Huntington's disease, argyrophilic grain dementia, senile dementia of the neurofibrillary tangle type, and brain tumors), and is a concept including cerebrovascular diseases such as degeneration of the central nerve due to intracerebral hemorrhage.
All-trans-retinoic acid (ATRA) is a type of cyclic retinoid having a six-membered carbon skeleton, and is known to act as a ligand of a retinoic acid receptor in a nucleus and exhibit anticancer activity. Furthermore, it has been reported that the ATRA and artificial analogs thereof exhibit an improving effect on Alzheimer's disease (AD), which has attracted great attention.
In order to develop a therapeutic drug targeting a brain for AD and the like, it is important to analyze intracerebral kinetics of a candidate compound for drug discovery, and positron emission tomography (PET) is extremely effective as means therefor. Therefore, the present inventors have published a study in which ATRA labeled with 11C is synthesized and used as a probe for PET (Non Patent Literature 1).
However, it has become clear that there are many problems as follows in using the 11C-labeled ATRA as a probe for PET.
1) There are many steps for synthesizing a labeled precursor. 2) The 11C-labeled ATRA is unstable under light or labeling reaction conditions. 3) A plurality of geometric isomers are generated in a 11C labeling process, and separation and purification thereof are complicated. 4) A large number of metabolites are generated in vivo, which makes intracerebral kinetics analysis complicated.
In order to solve these problems, some 11C-labeled artificial retinoids having a structure similar to ATRA and being relatively chemically stable have also been synthesized. For example, in Non Patent Literature 2, 11C-labeled Am80 was synthesized as a 11C-labeled artificial retinoid. However, when the 11C-labeled Am80 was used as a PET probe, no intracerebral migration was observed, and intracerebral kinetics analysis could not be conducted. Further, in Non Patent Literature 3, when 11C-labeled CBt-PMN was synthesized as a 11C-labeled artificial retinoid and used as a PET probe thereof, higher intracerebral migration than that of 11C-labeled bexarotene was observed. However, since a final yield in a case of total synthesis of the 11C-labeled CBt-PMN is as extremely low as 0.33%, image photographing by PET is used only for small animals, and intracerebral kinetics such as analysis of kinetics and intracerebral distribution has not been elucidated.
Note that Patent Literature 1 describes that peretinoin, which is a type of acyclic retinoid, can be used for anticancer agents. However, it has not been known at all so far that the peretinoin has intracerebral migration, and it has not been known either that the peretinoin activates a central nervous system (CNS) or can be used as an agent for central nervous system degenerative diseases. Further, conventionally known synthesis of the peretinoin requires many steps and takes time and effort.
The present invention has been made in view of the conventional problems described above, and an object of the present invention is to achieve any one of the following 1) and 2).
1) To provide a 11C-labeled acyclic retinoid that can be synthesized in a short time with a high yield and can be suitably used as a PET probe for elucidating intracerebral kinetics, a PET probe using it, and methods for producing them.
2) To provide a new method for producing an acyclic retinoid and a central nervous system activator containing the acyclic retinoid.
In order to solve the conventional problems described above, the present inventors have focused on acyclic retinoids having a chain-like carbon skeleton. Peretinoin, a type of acyclic retinoid, is known as a therapeutic drug for peripheral liver cancer, but there has been no reported example of acyclic retinoids migrating into a brain nor 11C-labeled acyclic retinoids. However, the acyclic retinoids have a ring-opened structure of a cyclic retinoid, and they are related to each other in terms of a chemical structure. Therefore, an attempt was made to label the acyclic retinoids with 11C and use them as a PET probe, instead of the cyclic retinoids.
As a result, it was surprisingly found that the acyclic retinoids migrated into the brain, and it was found to be suitable for elucidating the intracerebral kinetics. Moreover, these 11C-labeled acyclic retinoids also have an advantage of being easier to synthesize and more chemically stable than the cyclic retinoids.
In other words, a 11C-labeled acyclic retinoid of the present invention includes a compound represented by the following chemical formula (a) (in other words, peretinoin in which a methyl group at the 3-position is labeled with 11C), or an ester or a salt thereof.
A PET probe of the present invention is characterized by containing the 11C-labeled acyclic retinoid of the present invention described above. According to test results of the present inventors, since this PET probe has intracerebral migration, it can be used as a PET probe for elucidating intracerebral kinetics. The 11C-labeled acyclic retinoid of the chemical formula (a) having a carboxy group has a higher intracerebral migration rate than the 11C-labeled acyclic retinoid in which the carboxy group is esterified, and is particularly excellent as a PET probe.
The 11C-labeled acyclic retinoid of the present invention can be produced by cross-coupling a methyl iodide labeled with 11C and the following organotin compound (b) (provided that in the formula, —COOR1 represents an ester group, and R2 represents an alkyl group which may have a branch) in an aprotic solvent in the presence of a palladium complex, a phosphine ligand, and a cuprous halide. Furthermore, a carboxylic acid can be easily obtained by continuously hydrolyzing an ester group of a coupling product in the same reaction vessel. A strong base such as a potassium hydroxide and a sodium hydroxide can be used for the hydrolysis, and an acid such as a formic acid is added after the hydrolysis to obtain a free carboxylic acid.
In this production method, the phosphine ligand is sterically coordinated to the palladium complex in an unsaturated manner to create an active reaction field, and further, the methyl iodide labeled with 11C is oxidatively added to the palladium complex to which the phosphine ligand is coordinated to form a palladium complex in which the phosphine ligand is coordinated to [11C]CH3PdI.
On the other hand, the organotin compound (b) undergoes transmetalation with the cuprous halide to become an organocopper compound having high nucleophilicity.
Then, the palladium complex in which the phosphine ligand is coordinated to [11C]CH3PdI undergoes a substitution reaction with the organocopper compound, further a reductive elimination occurs, and a coupling reaction with [11C]CH3 is completed.
The palladium complex is not particularly limited, and for example, a palladium zero-valent complex obtained by reducing Pd(OAc)2, Pd2(dba)3, or the like can be used.
In addition, in the present description, the phosphine ligand refers to a compound having three organic substituents bonded to a trivalent phosphorus atom and further having an unshared electron pair. Specifically, examples of the phosphine ligand include trialkylphosphine such as trimethylphosphine, trioctylphosphine, tributylphosphine, and dimethyloctylphosphine, tri(o-tolyl)phosphine, tricyclohexylphosphine, trixylylphosphine, trimesitylphosphine, tris(tetramethylphenyl)phosphine, diphenyl-p-chlorophenylphosphine, tris (p-methoxyphenyl)phosphine, diphenylethylphosphine, dimethylphenylphosphine, bis(diphenylphosphino)methane, 1,2-bis(diphenylphosphino)ethane, dioctyloctoxyphosphine, dibutylbutoxyphosphine, diphenylphenoxyphosphine, ditolyltolyloxyphosphine, dixylylxylyloxyphosphine, diphenylethoxyphosphine, diethylphenoxyphosphine, octyldioctoxyphosphine, butylbutoxyphosphine, phenyldiphenoxyphosphine, tolylditolyloxyphosphine, xylyldixylyloxyphosphine, phenyldiethoxyphosphine, ethyldiphenoxyphosphine, trioctylphosphite, tributylphosphite, dimethyloctylphosphite, tricyclohexylphosphite, triphenylphosphite, tritolylphosphite, trixylylphosphite, diphenylethylphosphite, dimethylphenylphosphite, 1,3-bis(diphenylphosphino)propane (dppp), (2-biphenylyl)dicyclohexylphosphine, and tri-(1-naphthyl)phosphine.
As the aprotic solvent, a formamide solvent such as N,N-dimethylformamide and N,N-diethylformamide, an acetamide solvent such as N,N-dimethylacetamide and N,N-diethylacetamide, a pyrrolidone solvent such as N-methyl-2-pyrrolidone and N-vinyl-2-pyrrolidone, a sulfoxide solvent such as dimethylsulfoxide and diethylsulfoxide, hexamethylphosphoramide, γ-butyrolactone, and the like can be used. Among these, the formamide solvent, the acetamide solvent, and a pyrrolidone polar solvent are preferable.
Further, it is preferable to add at least one of a carbonate, an alkali metal fluoride, and an alkali metal salt of ascorbic acid in a coupling step. In this case, a trialkyltin halide produced as a by-product in the coupling step reacts with at least one of the carbonate, the alkali metal fluoride, and the alkali metal salt of ascorbic acid to neutralize or precipitate (precipitate as a trialkylstannyl carbonate in a case of the carbonate, as a trialkylstannyl fluoride in a case of the alkali metal fluoride, and as an ascorbate in a case of the alkali metal salt of ascorbic acid), and is thus excluded from a reaction system. Therefore, transmetalation from Sn to Cu is promoted, and a yield can also be increased.
A central nervous system activator of the present invention contains an acyclic retinoid (common name: peretinoin) represented by the following chemical formula (c), or an ester or a salt thereof as an active ingredient.
Since the 11C-labeled acyclic retinoid of the present invention has the intracerebral migration, peretinoin which is not labeled with 11C, or an ester or a salt thereof similarly has the intracerebral migration. Furthermore, according to the test results of the present inventors, the peretinoin which is the acyclic retinoid represented by the above chemical formula (c) and the ester thereof improve a motor function of a mouse after cerebral hemorrhage as well as suppress activity of microglia around a hematoma. From these facts, the peretinoin which is the acyclic retinoid represented by the above chemical formula (c), or the ester or the salt thereof can be used as a central nervous system (CNS) activator. Furthermore, it can be used in a therapeutic agent for central nervous system degenerative diseases, and can promote development of a new diagnostic and therapeutic drug as a lead compound for developing a therapeutic agent for central nervous system degenerative diseases.
A method for producing an acyclic retinoid of the present invention includes converting a phosphonic acid ester represented by the following chemical formula (d) (wherein R1 represents an alkyl group) and a carboxylic acid ester represented by the following chemical formula (e) into an acyclic retinoid of a chemical formula (f) (wherein —COOR2 represents an ester group) by the Horner-Wadsworth-Emmons reaction.
The phosphonic acid ester of the chemical formula (d) used in this production method can be easily obtained with a high yield by the Michaelis-Albuzov reaction using trans, trans-farnesol as a starting material, converting a hydroxyl group thereof into a chloride, and further using a trimethyl acid. Further, the carboxylic acid ester represented by the chemical formula (e) can be obtained while maintain the E configuration by converting hydroxyacetone into a hydroxyester of an E isomer by the Wittig olefin synthesis, and oxidizing it. Then, according to the production method of the present invention, the phosphonic acid ester of the following chemical formula (d) and an aldehyde of the chemical formula (e) are bonded with a carbon-carbon double bond by the HWE reaction, whereby the acyclic retinoid of the chemical formula (f) can be produced in fewer steps than the production method described in Patent Literature 1.
Furthermore, by hydrolyzing the acyclic retinoid of the chemical formula (f) thus obtained, the peretinoin can be obtained with a high stereoselective yield. For the hydrolysis, a strong base such as potassium hydroxide or sodium hydroxide can be used. After the hydrolysis, an acid such as a formic acid is added to obtain a free carboxylic acid.
The 11C-labeled acyclic retinoid obtained by the production method of the present invention has sufficient radioactivity to obtain a PET image, and can be used as a PET probe. Further, since the 11C-labeled acyclic retinoid of the present invention has a 11C-labeled position that is metabolically stable, a PET probe with high reproducibility and high quality is provided. Furthermore, since the hydrolysis of the ester group after the coupling step proceeds quickly and easily, synthesis by using a general automated radiopharmaceutical synthesis device is possible. Further, since the 11C-labeled acyclic retinoid of the present invention has cerebral migration, it serves as a useful support molecular tool in a process of developing a therapeutic agent for central nervous system degenerative diseases, for example, promoting development of a new diagnostic and therapeutic drug for intracerebral cancers of acyclic retinoids targeting glioma, which is a cancer of a central nervous system.
In addition, since the central nervous system activator of the present invention has an effect of migrating into the brain and activating the central nervous system, it can be used as a therapeutic agent for central nervous system degenerative diseases and a lead compound for development thereof.
Acyclic retinoids 4a, 4b, and 4c and peretinoin 3 (see the following chemical formulae), and their 11C-labeled compounds [11C]-4a, [11C]-4b, [11C]-4c, and [11C]-3 were synthesized through the following procedure. Note that the acyclic retinoids 4a, 4b, and 4c and the peretinoin 3 are acyclic retinoids contained in a central nervous system activator of the present invention, and the [11C]-4a, [11C]-4b, [11C]-4c, and [11C]-3 are 11C-labeled acyclic retinoids of the present invention. Details will be described below.
(Chemicals, Analyzers, and the Like Used in Examples)
All chemical substances and solvents except THF were purchased from Sigma-Aldrich Japan, Wako Pure Chemical Industries, Tokyo Chemical Industry, Kanto, and Nacalai Tesque and used as they were without purification. 1H, 13C, and 19F nuclear magnetic resonance (NMR) spectra were measured with a JEOL JNM 400-400 spectrometer. A chemical shift was set to 0.00 ppm based on TMS, and set to 7.26 for 1H NMR and 77.0 for 13C NMR based on CHCl3. Abbreviations s, d, t, q, and m denote singlet, doublet, triplet, quartet, and multiplet, respectively. High resolution mass spectra (HRMS) were measured with a PE Biosystems Mariner system and a JEOL JMS-700/GI. [11C]CO2 was produced by the 14N(p,α)11C nuclear reaction using a YPRIS HM-18 cyclotron (manufactured by Sumitomo Heavy Industries, Ltd.). For production of [11C]methyl iodide and 11C-labeled compounds, a unique automated radiolabeling system consisting of heating, dilution, HPLC injection, fractional recovery, and evaporation of a reaction mixture was used. Radioactivity was quantified with an ATOMLABTM500 dose calibrator (Biodex Medical Systems, Inc.). As an analytical HPLC system used for a [11C]methylated product, an HPLC system equipped with an amplifier and bias power supply 925-SCINT (AMETEK Co., Ltd.), a linear count rate meter 7131-1 (Ohyo Koken Kogyo Co. Ltd.), a Shimadzu system controller (SPD-10Avp), an online degasser (DGU-12A), a solvent delivery unit (LC-10ATvp), a column oven (CTO-10A), and a photodiode array detector (SCL-10Avp), and software (LC solution) were employed. As columns used for analytical and preparative HPLC, CAPCELL PAK C18 4.6×250 mm and CAPCELL PAK C18 MG 5 μm 10×250 mm (manufactured by Shiseido Company, Limited) were used.
An acyclic retinoid 14a and an acyclic retinoid 14b were synthesized by the following synthesis method.
Trans, trans-farnesyl bromide 13 (2.7 mL, 10 mmol), triethyl phosphite (1.9 mL, 11 mmol), and dry toluene (20 mL) were put in a 50 mL round-bottom flask. A mixture was refluxed for 20 hours and then returned to room temperature. A solution was concentrated under reduced pressure, and a residue was purified by silica gel column chromatography (hexane/acetone=5:1 to ½) to obtain the compound 14b (2.82 g, 8.23 mmol, 82%) as a yellow oil.
1H NMR (400 MHz, CDCl3), 1.30 (t, J=7.0 Hz, 6H, 2CH3), 1.59 (s, 6H, 2CH3), 1.65 (d, J=3.6 Hz, 3H, CH3), 1.67 (s, 3H, CH3), 1.89-2.14 (m, 8H, 4CH2), 2.56 (dd, J=7.6, 2J(1H-31P)=21.6 Hz, 2H, CH2), 4.00-4.16 (m, 4H, 2CH2), 5.03-5.13 (m, 2H, 2C═CH), 5.14-5.24 (m, 1H, C═CH); 13C NMR (100 MHz, CDCl3), 16.06, 16.40, 16.56 (d, 3J(13C-31P)=5.7 Hz, 2C), 17.76, 25.76, 26.49, 26.53, 26.46 (d, 1J(13C-31P)=139.2 Hz), 26.81, 39.78, 61.81 (d, 2J(13C-31P)=5.8 Hz, 2C), 112.51 (d, 2J(13C-31P)=11.5 Hz), 123.88, 124.39, 131.39, 135.37, 140.34 (d, 3J(13C-31P)=13.4 Hz); HR-MS (EI+,100% acetone): m/z: calcd for C19H35O3P ([M-C4H9]+) 342.2324; found, 342.2319.
The compound 14a was obtained by a method similar to the method for synthesizing the compound 14b, using trimethyl phosphite instead of triethyl phosphite.
The compounds 4a, 4b, and 4c were synthesized by the following synthesis method.
An (E)-3-methyl-4-oxo-2-butenoic acid ethyl ester was synthesized from an (E)-4-hydroxy-3-methyl-2-butenoic acid ethyl ester (72.2 mg, 0.500 mmol) based on a known document (G. E. Magoulas, et. al. J. Med. Chem. 2011, 46, 721-737). The (E)-3-methyl-4-oxo-2-butenoic acid ethyl ester thus obtained was used without purification. The compound 14b (171 mg, 0.500 mmol) and dry THF (2 mL) were put in a 30 mL two-neck round-bottom flask and cooled to −78° C. in a dry ice/acetone bath, and at this temperature, bis(trimethylsilyl)amide potassium (1.0 M THF solution, 500 μL, 0.5 mmol) was added. A reaction mixture was stirred for 1 hour, and then the (E)-3-methyl-4-oxo-2-butenoic acid ethyl ester dissolved in THF (2 mL) was dropped at −78° C. After stirred at room temperature for 1 hour, an obtained mixture was cooled to 0° C., saturated aqueous NH4Cl (2 mL) was added, and the mixture was extracted with diethyl ether (3×5 mL). An organic layer was washed with water (10 mL) and dried with anhydrous sodium sulfate, and then a solvent was distilled off under reduced pressure. A crude product was purified by the silica gel column chromatography {hexane/ethyl acetate=50:1} to obtain an acyclic retinoid 4b (43 mg, 0.13 mmol, 26%) as a yellow oil.
1H NMR (400 MHz, CDCl3), 1.27 (t, J=7.2 Hz, 3H, CH3), 1.58 (s, 3H, CH3), 1.59 (d, J=0.8 Hz, 3H, CH3), 1.66 (d, J=0.8 Hz, 3H, CH3), 1.84 (d, J=0.8 Hz, 3H, CH3), 1.90-2.20 (m, 8H, 4CH2), 2.31 (d, J=1.2 Hz, 3H, CH3), 4.15 (q, J=7.2 Hz, 2H, CH2), 5.00-5.20 (m, 2H, 2C═CH), 5.73 (s, 1H, C═CH), 5.95 (d, J=11.2 Hz, 1H, C═CH), 6.16 (d, J=15.6 Hz, 1H, C═CH), 6.83 (dd, J=15.6 11.2, Hz, 1H, C═CH); 13C NMR (100 MHz, CDCl3), 13.88, 14.44, 16.10, 17.24, 17.77, 25.78, 26.49, 26.77, 39.76, 40.33, 59.67, 118.12, 123.62, 124.35, 125.02, 131.10, 131.43, 133.52, 135.70, 143.97, 153.09, 167.33; HR-MS (EI+, 100% acetone): m/z: calcd for C22H34C2([M-C4H9]+) 330.2559; found, 330.2561.
A compound (4a) was synthesized from the compound 14b (35.5 mg, 0.104 mmol) and an (E)-3-methyl-4-oxo-2-butenoic acid methyl ester ((E)-4-hydroxy-3-methyl-2-butenoic acid. The procedure was performed in the same manner as the method for synthesizing the acyclic retinoid (4b), and a compound 4a (18.0 mg, 56.9 μmol, 55%) was obtained as a yellow oil.
1H NMR (400 MHz, CDCl3), 1.58 (s, 3H, CH3), 1.59 (d, J=0.8 Hz, 3H, CH3), 1.66 (s, 3H, CH3), 1.84 (s, 3H, CH3), 1.90-2.20 (m, 8H, 4CH2), 2.32 (d, J=0.8 Hz, 3H, CH3), 3.69 (s, 3H, CH3), 5.00-5.20 (m, 2H, 2C═CH), 5.73 (s, 1H, C═CH), 5.95 (d, J=11.6 Hz, 1H, C═CH), 6.17 (d, J=14.8 Hz, 1H, C═CH), 6.83 (dd, J=14.8, 11.6 Hz, 1H, C═CH); 13C NMR (100 MHz, CDCl3), 13.90, 16.10, 17.26, 17.77, 25.78, 26.49, 26.77, 39.76, 40.33, 51.01, 117.58, 123.61, 124.35, 125.00, 131.29, 131.45, 133.43, 135.71, 144.15, 153.43, 167.75; HR-MS (EI+, 100% acetone): m/z: calcd for C21H32O2 (M+) 316.2402; found, 316.2415.
An 4,5-Didehydrogeranylgeranoic acid (18.2 mg, 60.2 μmol) and dry DMF (600 μL) were put in a 5 mL test tube, and K2CO3 (12 mg, 90 μmol) and benzyl bromide (7.8 μL, 11 mg, 66 μmol) were added. After stirred at 50° C. for 2 hours, an obtained mixture was cooled to room temperature. The mixture was extracted with diethyl ether (3×3 mL), and an extract was washed with saturated brine and dried with anhydrous sodium sulfate. An extraction liquid was concentrated under reduced pressure, and a residue was purified by the silica gel column chromatography {hexane/acetone=100:1} to obtain a compound 4c (16.8 mg, 42.8 μmol, 71%) as a yellow oil.
1H NMR (400 MHz, CDCl3), 1.58 (s, 3H, CH3), 1.59 (s, 3H, CH3), 1.67 (s, 3H, CH3), 1.84 (s, 3H, CH3), 1.90-2.20 (m, 8H, 4CH2), 2.34 (s, 3H, CH3), 5.02-5.14 (m, 2H, 2C═CH), 5.15 (s, 2H, CH2), 5.79 (s, 1H, C═CH), 5.95 (d, J=11.2 Hz, 1H, C═CH), 6.16 (d, J=15.2 Hz, 1H, C═CH), 6.89 (dd, J=15.2, 11.2 Hz, 1H, C═CH), 7.26-7.42 (m, 5H, C6H5); 13C NMR (100 MHz, CDCl3), 14.01, 16.11, 17.27, 17.77, 25.78, 26.49, 26.77, 39.76, 40.33, 65.61, 117.63, 123.60, 124.34, 125.01, 128.12, 128.23 (2C), 128.59 (2C), 131.42 (2C), 133.40, 135.73, 136.57, 144.30, 153.89, 167.05; HR-MS (EI+, 100% acetone): m/z: calcd for C27H36O2(M+) 392.2715; found, 392.2734.
Organotin compounds 15a, 15b, and 15c were synthesized by the following synthesis route.
An (2Z)-3-bromo-4-hydroxy-2-butenoic acid ethyl ester (8b) was synthesized according to the procedure of a known document (M. Shengming, L. Xiyan, Org. Synth. 1995, 72, 112-115). 5a (1.56 g, 7.46 mmol) and dry DMF (10 mL) were put in a 250 mL round-bottom flask and cooled to 0° C., and imidazole (663 mg, 9.75 mmol) and tert-butyldimethylsilyl chloride (1.24 g, 8.25 mmol) were added. After stirred at room temperature for 4 hours, a reaction mixture was quenched with water (10 mL) and extracted with diethyl ether (3×10 mL). An organic layer was washed with water (30 mL) and dried with anhydrous sodium sulfate, and then a solvent was evaporated under reduced pressure. A crude product was subjected to the silica gel column chromatography {hexane/ethyl acetate=10:1} to obtain a compound 9b (2.40 g, 7.41 mmol, 99%) as a yellow oil.
1H NMR (400 MHz, CDCl3), 0.10 (s, 6H, 2CH3), 0.92 (s, 9H, 3CH3), 1.30 (t, J=7.2 Hz, 3H, CH3), 4.22 (q, J=7.2 Hz, 2H, CH2), 4.29 (d, J=2.4 Hz, 2H, CH2), 6.69 (t, J=2.4 Hz, 1H, C═CH); 13C NMR (100 MHz, CDCl3), −5.38 (2C), 14.30, 18.38, 25.84 (3C), 60.66, 68.50, 117.16, 139.42, 164.59; HR-MS (EI+, 100% acetone): m/z: calcd for C8H1479BrO3Si ([M-C4H9]+) 264.9896; found, 264.9905.
Copper cyanide (537 mg, 6 mmol) and dry THF (10 mL) were put in a 50 mL two-neck round-bottom flask, an n-butyllithium solution (1.89 M hexane solution, 3.17 mL, 6.00 mmol) was dropped at −78° C., and a mixture was stirred for 15 minutes. Bis(tributyltin) (3.03 mL, 6.00 mmol) was dropped into the mixture at −78° C. After the mixture was stirred at 0° C. for 1 hour, a solution of bromide 9b (646 mg, 2.00 mmol) in dry THF (10 mL) was added to an obtained mixture of copper ate complexes at −78° C., and the mixture was stirred for 30 minutes. The mixture was quenched with brine, NH4Cl aq (10 mL), and 28% NH4OH at −78° C. An obtained mixture was extracted with diethyl ether (3×10 mL), and an extract was washed with water (30 mL) and dried with anhydrous sodium sulfate. A solvent was evaporated under reduced pressure. A crude product was subjected to the silica gel column chromatography {hexane/ethyl acetate=150:1} to obtain a compound 10b (995 mg, 1.87 mmol, 94%) as a yellow oil.
1H NMR (400 MHz, CDCl3), 0.07 (s, 6H, 2CH3), 0.86 (t, J=7.2 Hz, 9H, 3CH3), 0.90-0.98 (m, 6H, 3CH2), 0.92 (s, 9H, 3CH3), 1.20-1.34 (m, 9H, 3CH2 and CH3), 1.35-1.51 (m, 6H, 3CH2), 4.18 (q, J=7.2 Hz, 2H, CH2), 4.26-2.26 (m, 2H, CH2), 6.56-6.82 (m, 1H, C═CH); 13C NMR (100 MHz, CDCl3),−5.32 (2C), 10.89 (3C), 13.81 (3C), 14.43, 18.61, 26.05 (3C), 27.48 (3C), 29.29 (3C), 60.32, 68.70, 124.70, 168.40, 171.93; HR-MS (EI+, 100% acetone): m/z: calcd for C20H41O3Si120Sn ([M-C4H9]+) 477.1847; found, 477.1824.
10b (687 mg, 1.29 mmol) and dry DMSO (4 mL) were put in a 50 Ml round-bottom flask, and 18-crown-6-ether (1.03 g, 3.90 mmol) and potassium fluoride (226 mg, 3.89 mmol) were added at 0° C. After a mixture was stirred at room temperature for 3.5 hours, a phosphate buffer (pH 7.4) was added at 0° C., the mixture was extracted with diethyl ether (3×10 mL), an extract was washed with water (30 mL) and dried with anhydrous sodium sulfate, and a solvent was evaporated under reduced pressure. A crude product was separated by the silica gel column chromatography {hexane/ethyl acetate=15:1} to obtain a compound 11b (425 mg, 1.01 mmol, 78%) as a yellow oil.
1H NMR (400 MHz, CDCl3), 0.86 (t, J=7.2 Hz, 9H, 3CH3), 0.90-1.07 (m, 6H, 3CH2), 1.20-1.34 (m, 9H, 3CH2 and CH3), 1.34-1.54 (m, 6H, 3CH2), 1.61 (t, J=6.0 Hz, 1H, OH), 4.18 (q, J=7.2 Hz, 2H, CH2), 4.44-4.49 (m, 2H, CH2), 6.53-6.80 (m, 1H, C═CH); 13C NMR (100 MHz, CDCl3), 10.97 (3C), 13.79 (3C), 14.40, 27.48 (3C), 29.25 (3C), 60.47, 68.56, 124.77, 168.06, 172.25; HR-MS (EI+, 100% acetone): m/z: calcd for C14H27O3120Sn ([M-C4H9]+) 363.0982; found, 363.1002.
11b (168 mg, 0.401 mmol) and hexane (3 mL) were put in a 20 mL round bottom of a flask. MnO 2 (1.04 g, 12.0 mmol) was added to a solution. An obtained suspension was stirred at 40° C. for 1.5 hours and then passed through a pad of Celite. When a filtrate was evaporated, a (Z)-4-oxo-3-(tributylstannyl)-2-butenoic acid ethyl ester (12b) was obtained as a yellow oil. 12b was used without further purification. 1 (137 mg, 0.328 mmol) and dry THF (2 mL) were put in a 30 mL two-neck round bottom of a flask. The solution was cooled to −78° C. in a dry ice/acetone bath, and bis(trimethylsilyl) amide potassium (1.0 M THF solution, 400 μL, 0.4 mmol) was added at this temperature. A reaction mixture was stirred for 1 hour, and 12b dissolved in THF (2 mL) was dropped at −78° C. After stirred at RT for 1 hour, an obtained mixture was cooled to 0° C., a phosphate buffer (pH 7.4) was added, and the mixture was extracted with diethyl ether (3×5 mL). An organic layer was washed with water (10 mL) and dried with anhydrous sodium sulfate. A solvent was evaporated under reduced pressure. A crude product was subjected to the silica gel column chromatography (hexane only) to obtain a compound 15b (18.6 mg, 30.7 μmol, 7.7%) as a yellow oil.
1H NMR (400 MHz, CDCl3), 0.86 (t, J=7.4 Hz, 9H, 3CH3), 0.91-1.12 (m, 6H, 3CH2), 1.20-1.35 (m, 9H, 3CH2, CH3), 1.35-1.57 (m, 6H, 3CH2), 1.59 (s, 3H, CH3), 1.60 (s, 3H, CH3), 1.67 (s, 3H, CH3), 1.82 (s, 3H, CH3), 1.90-2.20 (m, 8H, 4CH2), 4.17 (q, J=7.4 Hz, 2H, CH2), 5.00-5.20 (m, 2H, 2C═CH), 5.92 (d, J=10.8 Hz, 1H, C═CH), 6.44 (d, J=15.6 Hz, 1H, C═CH), 6.46 (s, 1H, C═CH), 6.71 (dd, J=15.6, 11.4 Hz, 1H, C═CH); 13C NMR (100 MHz, CDCl3), 12.43 (3C), 13.79 (3C), 14.46, 16.10, 17.24, 17.77, 25.78, 26.57, 26.79, 27.48 (3C), 29.21 (3C), 39.78, 40.29, 60.30, 123.74, 124.39, 125.44, 127.73, 131.43, 132.36, 135.62, 136.21, 142.94, 168.33, 168.42; HR-MS (EI+, 100% acetone): m/z: calcd for C29H49O2120Sn ([M-C4H9]+) 549.2755; found, 549.2747.
15a was synthesized from a (2E,6E)-3,7,11-trimethyl-2,6,10-(dodecatrienyl) phosphonic acid dimethyl ester (14a) (11.7 mg, 37.2 μmol) and (Z)-4-oxo-(13.8 mg, 34.2 μmol) (7.9 mg, 13.4 μmol, 39%). In addition, from a 3-(tributylstannyl)-2-butenoic acid methyl ester ((Z)-4-hydroxy-3-(tributylstannyl)-2-butenoic acid methyl ester (127 mg, 0.313 mmol), 15b as a yellow oil was obtained by a similar procedure (10.0 mg, 17.0 μmol, 15%).
1H NMR (400 MHz, CDCl3), 0.86 (t, J=7.4 Hz, 9H, 3CH3), 0.93-1.10 (m, 6H, 3CH2), 1.24-1.33 (m, 6H, 3CH2), 1.42-1.51 (m, 6H, 3CH2), 1.585 (s, 3H, CH3), 1.594 (s, 3H, CH3), 1.67 (s, 3H, CH3), 1.82 (s, 3H, CH3), 1.93-2.26 (m, 8H, 4CH2), 3.72 (s, 3H, CH3), 5.06-5.10 (m, 2H, 2C═CH), 5.92 (d, J=10.8 Hz, 1H, C═CH), 6.44 (d, J=15.6 Hz, 1H, C═CH), 6.46 (s, 1H, C═CH), 6.71 (dd, J=15.6, 11.4 Hz, 1H, C═CH); 13C NMR (100 MHz, CDCl3), 12.40 (3C), 13.81 (3C), 16.10, 17.26, 17.77, 25.80, 26.55, 26.79, 27.48 (3C), 29.21 (3C), 39.80, 40.29, 51.56, 123.72, 124.37, 125.42, 127.33, 131.43, 132.51, 135.64, 136.23, 143.13, 168.67, 168.78; HR-MS (EI+, 100% acetone): m/z: calcd for C28H47O3120Sn ([M-C4H9]+) 535.2598; found, 535.2603.
A mixture of 15a and 15b (1:1 molar ratio, 22.7 mg, 38.4 μmol), methanol (200 μL), and 2-propanol (200 μL) were put in a 5 mL test tube. After addition of 10 M KOH in CH3OH/H2O (2:1, v/v), 100 μL, 1.00 mmol, a reaction mixture was stirred at 100° C. for 30 minutes. After neutralization with a formic acid (1 mL), an obtained mixture was extracted with diethyl ether (3×3 mL), and an extract was washed with a brain (10 mL) and dried with anhydrous sodium sulfate. A solvent was evaporated under reduced pressure. A crude product was subjected to the silica gel column chromatography {hexane/ethyl acetate=10:1} to obtain a yellow oil (15.6 mg, 27.0 μmol, 71%).
1H NMR (400 MHz, CDCl3), 0.86 (t, J=7.4 Hz, 9H, 3CH3), 0.91-1.12 (m, 6H, 3CH2), 1.20-1.35 (m, 6H, 3CH2), 1.35-1.54 (m, 6H, 3CH2), 1.59 (s, 3H, CH3), 1.60 (s, 3H, CH3), 1.67 (s, 3H, CH3), 1.83 (s, 3H, CH3), 1.90-2.20 (m, 8H, 4CH2), 5.02-5.20 (m, 2H, 2C═CH), 5.93 (d, J=11.6 Hz, 1H, C═CH), 6.47 (d, J=16.0 Hz, 1H, C═CH), 6.50 (s, 1H, C═CH), 6.71 (dd, J=16.0, 11.6 Hz, 1H, C═CH), 8.82-9,78 (br s, 1H, COOH).
An (2E,4E,6E,10E)-7,11,15-trimethyl-3-(tributylstannyl)-2,4,6,10,14-hexadecapentaenoic acid (15.6 mg, 27.0 μmol) and dry DMF (200 μL) in a 5 mL test tube. K2CO3 (5.60 mg, 40.5 μmol) and benzyl bromide (3.50 μL, 5.08 mg, 29.7 μmol) were added to a solution. After stirred at 50° C. for 1 hour, an obtained mixture was cooled to room temperature. The mixture was extracted with diethyl ether (3×3 mL), and an extract was washed with brine (10 mL) and dried with anhydrous sodium sulfate. The solution was concentrated under reduced pressure, and a residue was purified by the silica gel column chromatography {hexane/acetone=100:1} to obtain a compound 15c (13.5 mg, 20.2 μmol, 75%) as a yellow oil.
1H NMR (400 MHz, CDCl3), 0.86 (t, J=7.6 Hz, 9H, 3CH3), 0.91-1.12 (m, 6H, 3CH2), 1.20-1.35 (m, 6H, 3CH2), 1.35-1.54 (m, 6H, 3CH2), 1.58 (s, 3H, CH3), 1.59 (d, J=0.8 Hz, 3H, CH3), 1.66 (s, 3H, CH3), 1.81 (s, 3H, CH3), 1.90-2.19 (m, 8H, 4CH2), 5.02-5.14 (m, 2H, 2C═CH), 5.17 (s, 2H, CH2), 5.91 (d, J=11.6 Hz, 1H, C═CH), 6.44 (d, J=15.2 Hz, 1H, C═CH), 6.52 (s, 1H, C═CH), 6.72 (dd, J=15.2, 11.6 Hz, 1H, C═CH), 7.27-7.42 (m, 5H, C6H5); 13C NMR (100 MHz, CDCl3), 12.45 (3C), 13.83 (3C), 16.10, 17.28, 17.77, 22.80, 26.55, 26.79, 27.50 (3C), 29.22 (3C), 39.80, 40.31, 66.10, 123.72, 124.37, 125.44, 127.29, 128.18, 128.32 (2C), 128.59 (2C), 131.43, 132.63, 135.64, 136.16, 136.38, 143.25, 168.23, 169.37; HR-MS (EI+, 100% acetone): m/z: calcd for C34H51O2120Sn ([M-C4H9]+) 611.2911; found, 611.2915.
The [11C]-4a, [11C]-4b, [11C]-4c, and [11C]-3 were synthesized by the following synthesis method.
Synthesis of (2E,4E,6E,10E)-3,7,11,15-tetramethyl-2,4,6,10,14-hexadecapentaenoic acid ethyl ester 4b by C-methylation using nonradioactive methyl iodide [Pd2(dba)3] (11.5 mg, 12.6 μmol), P(o-tolyl)3 (27.7 mg, 91.1 μmol), CuBr (6.50 mg, 45.3 μmol), and CsF (17.3 mg, 114 μmol) were put in a 1 mL Schlenk flask with argon substitution. A DMF (2 mL) solution of a stannyl precursor 15b (13.3 mg, 22.0 μmol) was transferred to the Schlenk flask through a stainless steel cannula at −10° C., after addition of methyl iodide (220 μL, 0.10 M in DMF, 22 μmol), a mixture was heated at 60° C. for 1 hour, and then an obtained mixture was quenched with water and extracted with diethyl ether (3×3 mL). Extracted organic layers were combined, washed with water and saturated brine, dried with anhydrous sodium sulfate, filtered, and concentrated. A crude product was separated by the silica gel column chromatography {hexane/ethyl acetate=100:1} to obtain the acyclic retinoid 4b as a light yellow oil (5.80 mg, 17.5 μmol, 80%).
1H NMR (400 MHz, CDCl3), 1.27 (t, J=7.2 Hz, 3H, CH3), 1.58 (s, 3H, CH3), 1.59 (d, J=0.8 Hz, 3H, CH3), 1.66 (d, J=0.8 Hz, 3H, CH3), 1.84 (d, J=0.8 Hz, 3H, CH3), 1.90-2.20 (m, 8H, 4CH2), 2.32 (d, J=0.8 Hz, 3H, CH3), 4.15 (q, J=7.2 Hz, 2H, CH2), 5.00-5.17 (m, 2H, 2C═CH), 5.73 (s, 1H, C═CH), 5.95 (d, J=11.2 Hz, 1H, C═CH), 6.16 (d, J=15.6 Hz, 1H, C═CH), 6.83 (dd, J=15.6, 11.2 Hz, 1H, C═CH).
[Pd2(dba)3] (1.0 mg, 1.1 μmol), P(o-tolyl)3 (2.4 mg, 8 μmol), CuBr (0.6 mg, 4 μmol), CsF (1.5 mg, 10 μmol), and a stannyl precursor 15c (1.1 mg, 1.6 μmol) in DMF (400 μL) were maintained below −10° C. by cooling while waiting for preparation of [11C] CH3I. [11C] CH3I formed from [11C] CO2 using the conventional LiAlH4 method was trapped in a reaction mixture, the cooling was stopped, and then the mixture was rapidly heated to 65° C. and bubbled after left for 2 minutes. For 2 minutes with a N2 gas. To avoid bumping, the reaction mixture was cooled for 10 seconds. After dilution with CH3CN (600 μL), the mixture was passed through a fine filter F (F162, Forte Grow Medical co., ltd.) with quartz glass wool (Tosho co., ltd.), and preparative HPLC (mobile phase, CH3CN only, column, CAPCELL PAK C18 UG 120 5 μm, 10 mm (inner diameter)×250 mm, flow rate, 5 mL/min, UV detection, 280 nm, retention time, 7.37 to 8.31 minutes). An HPLC analysis yield was calculated at 97% from a radio-HPLC peak area of the reaction mixture. Desired fractions were collected in a flask, and an organic solvent was removed under reduced pressure. A desired radioactive tracer was dissolved in a 0.25% solution of polysorbate 80 in saline (3.0 mL). Total synthesis time including purification by HPLC and preparation of radiopharmaceutical formulation for intravenous administration was 31 minutes. Separated radioactivity was 2.87 GBq at an end of synthesis, and specific radioactivity was 144 GBq μmol−1. A decay-corrected radiochemical yield was 57%, which was calculated based on radioactivity of [11C]CH3I trapped in the solution. Identification of [11C]4c was confirmed by co-injection with a real sample of non-radiolabeled 4c by analytical HPLC (mobile phase, CH3CN only, column, CAPCELL PAK C 18, 4.6 mm (id)×250 mm, flow rate, 1 mL/min; UV detection, 280 nm; retention time, 8.0 minutes). Chemical and radiochemical purities analyzed at 280 nm were >99% and >97%, respectively.
[Pd2(dba)3] (1.0 mg, 1.1 μmol), P(o-tolyl)3 (2.4 mg, 8 μmol), CuBr (0.6 mg, 4 μmol), CsF (1.5 mg, 10 μmol), and a stannyl precursor 15a (0.9 mg, 1.5 μmol) in DMF (300 μL) were maintained below −10° C. by cooling while waiting for preparation of [11C] CH3I. [11C] CH3I formed from [11C] CO2 using the conventional LiAlH4 method was trapped in a reaction mixture, the cooling was stopped, and then the mixture was rapidly heated to 65° C. and bubbled after left for 2 minutes. For 2 minutes with a N2 gas. To avoid bumping, the reaction mixture was cooled for 10 seconds. After an 8M KOHaq. (500 μL) solution was added to the mixture, the N2 gas was bubbled at 100° C. for 6 minutes. After cooling for 10 seconds, the reaction mixture was diluted with CH3CN/CH3CN/H2O (60:36:4 v/v/v, 1 mL) containing sodium ascorbate (2.2 mg, 11 μmol), the fine filter F (F162, Forte Grow Medical co., ltd.) and quartz glass wool (Tosoh co., ltd.) were used for the mixture, and preparative HPLC (mobile phase, CH3CN/0.2% HCOOH in water=90:10; column), CAPCELL PAK C18 UG 120 5 μm, 10 (id)×250 mm, flow rate, 5 mL/min, UV detection, 280 nm, retention time, 8:45 to 9:32 minutes). An HPLC analysis yield (%) was determined from a radio-HPLC peak area of the reaction mixture. Desired fractions were collected in a flask, and an organic solvent was removed under reduced pressure. A desired radioactive tracer was dissolved in a 0.25% solution of polysorbate 80 in saline (3.0 mL). Total synthesis time including HPLC purification and preparation of radiopharmaceutical formulation for intravenous administration was 40 minutes. Separated radioactivity was 0.94 GBq at an end of synthesis, and specific radioactivity was 186 GBq μmol−1. A decay-corrected radiochemical yield was 25%, which was calculated based on radioactivity of [11C]CH3I trapped in the solution. Chemical identity of [11C] 3 was confirmed by co-injection with a sample of non-radiolabeled 3 by analytical HPLC (mobile phase, CH3CN/0.2% HCOOH aqueous solution=90:10, column, CAPCELL PAK C18, 4.6 (id)×250 mm, flow rate, 1 mL/min, UV detection, 280 nm, retention time, 7.7 min). Chemical and radiochemical purities analyzed at 280 nm were 83% and 99%, respectively.
Radioactivity, molar activity of radioactivity, and decay-corrected radiochemical yield of the [11C]-4a, [11C]-4b, [11C]-4 c, and [11C]-3 synthesized as described above are shown in Table 1. From this table, it is found that the [11C]-4a, [11C]-4b, [11C]-4c, and [11C]-3 have sufficient radioactivity for a PET probe.
The peretinoin 3, which was not labeled with 11C, was synthesized from phosphate 14a, which served as a precursor when the [11C]-3 was synthesized, by applying the Horner-Wadsworth-Emmons (HWE) reaction, which allows formation of olefins that are highly selective about an E-body.
First, trans, trans-farnesol 16 was converted into a chloride 17 (a yield of 51%), and then the phosphate 14a was synthesized in a yield of 64% by the Michaelis-Albuzov reaction using trimethyl phosphite.
On the other hand, an aldehyde form 20, which was another compound for target acyclic retinoid synthesis, was synthesized as follows. In other words, a hydroxy ester 19 of an E isomer was synthesized at a yield of 57% by the Wittig olefin synthesis using hydroxyacetone 18 as a starting material, and the aldehyde form 20 maintaining the E configuration was synthesized at a yield of 43% by oxidation using manganese dioxide.
The phosphate 14a and the aldehyde 20 synthesized by the synthesis methods described above were bonded with a carbon-carbon double bond by the HWE reaction to obtain the peretinoin 3. In other words, potassium hexamethyldisilazide (KHMDS) was selected as a base for adjusting ylide from 14a, a methyl ester form 4a of 4,5-didehydro GGA (the peretinoin) was synthesized under a condition of 14a/KHMDS/14 (1:1:1 molar ratio), and this was hydrolyzed with a strong base to synthesize the target peretinoin 3 in three steps at a yield of 26%.
The procedure of synthesis and analytical data of the peretinoin 3 are described in detail below.
1H NMR (400 MHz, CDCl3): δ=1.60 (s, 6H, 2CH3), 1.68 (s, 3H CH3), 1.73 (s, 3H, CH3), 1.98-2.11 (m, 8H, 4CH2), 4.10 (d, J=7.6 Hz, CH2), 5.06-5.14 (m, 2H, olefine), 5.45 (t, J=7.6 Hz, olefine).
1H NMR (400 MHz, CDCl3): δ=1.60 (s, 6H, 2CH3), 1.66 (s, 3H, CH3), 1.69 (s, 3H, CH3), 1.95-2.10 (m, 8H, 4CH2), 2.58 (dd, J=22.0, 7.6 Hz, CH2), 3.74 (d, J=10.4 Hz, 6H, 2CH3O), 5.08-5.20 (m, 3H, olefines); 13C NMR (100 MHz, CDCl3) 5=16.00, 16.26 (d, JP-C=1.9 Hz) 17.67, 25.45 (d, JP-C=139.2 Hz), 25.69 (2C), 26.35 (d, JP-C=3.8 Hz), 26.70, 39.69, 52.57 (d, JP-C=6.7 Hz, 2C), 111.98 (d, JP-C=10.5 Hz), 123.72, 124.28, 131.32, 135.32, 140.57 (d, JP-C=14.3 Hz).
Hydroxyacetone (6.16 mL, 6.65 g, 89.8 mmol), benzene (90 mL), and methyl (triphenylphosphoranylidene) acetate (30.0 g, 89.8 mmol) were added to a 300-mL recovery flask at room temperature in order, and heated and refluxed for 24 hours. After concentration under reduced pressure, an obtained residue was purified by the silica gel column chromatography (hexane/ethyl acetate=3/1) to obtain a yellow oily target product 19 (6.63 g, 51.0 mmol, 57%).
1H NMR (400 MHz, CDCl3): δ=2.10 (s, 3H, CH3), 3.72 (s, 3H, CH3), 4.15-4.16 (m, 2H, CH2), 5.99-6.01 (m, 1H, olefine); 13C NMR (100 MHz, CDCl3): δ=15.60, 51.01, 67.04, 113.24, 157.50 167.19.
13 (6.45 g, 49.6 mmol) was added to a 1-L recovery flask and dissolved in hexane (500 mL), then manganese dioxide (129 g, 1.48 mol) was added, and a mixture was stirred at room temperature for 1 hour. Thereafter, an oxidant was removed by Celite filtration, a filtrate was concentrated under reduced pressure, and then an obtained residue was purified by the silica gel column chromatography (hexane/ethyl acetate=3/1) to obtain a yellow oily target product 20 (2.75 g, 21.5 mmol, 43%).
1H NMR (400 MHz, CDCl3): δ=2.18 (d, 1.2 Hz, 3H, CH3), 3.84 (s, 3H, CH3), 6.52 (t, 1.2 Hz, 1H, olefine), 9.57 (s, 1H, CHO); 13C NMR (100 MHz, CDCl3): δ=10.85, 52.00, 135.00, 150.70, 165.92, 194.43.
A THF (170 mL) solution of 14a (6.12 g, 19.5 mmol) was added to two 250-mL Schlenks under an argon atmosphere, and potassium hexamethyldisilazane (1.0 M THF solution, 19.5 mL, 19.5 mmol) was added at −78° C. After a mixture was stirred for 30 minutes, 20 (2.49 g, 19.5 mmol) dissolved in THF (30 mL) was added at −78° C., and then the mixture was stirred for 15 hours while the temperature was being gradually raised to room temperature. Then, under ice cooling, a saturated aqueous ammonium chloride solution (100 mL) was added, and the mixture was extracted with ethyl acetate (3×40 mL). Subsequently, an organic layer was washed with saturated saline (100 mL) and dried with anhydrous sodium sulfate. After concentration under reduced pressure, an obtained residue was purified by the silica gel column chromatography (hexane/ethyl acetate=50/1) to obtain a crude product (3.91 g).
The crude product (3.91 g) was added to a 100-mL recovery flask and dissolved in 2-propanol (24 mL), then a 10 M aqueous potassium hydroxide solution (40 mL) was added, and the mixture was heated and refluxed for 1.5 hours. Thereafter, a formic acid (32 mL) was added under ice cooling, and the mixture was concentrated under reduced pressure and then extracted with ethyl acetate (3×20 mL). Subsequently, the organic layer was washed with saturated saline (40 mL) and dried with anhydrous sodium sulfate. After concentration under reduced pressure, it was dissolved in hexane (8.7 ml) and crystallized at −20° C. A filtrate was concentrated and concentrated under reduced pressure, and then an obtained residue was purified by the silica gel column chromatography (hexane/ethyl acetate=10/1) and combined with a product by recrystallization to obtain the peretinoin 3 (1.50 g, 4.97 mmol, 26%, 3 steps) as a pale yellow needle-like crystal.
1H NMR (400 MHz, CDCl3): δ=1.60 (s, 3H, CH3), 1.61 (s, 3H, CH3), 1.68 (s, 3H, CH3), 1.86 (s, 3H, CH3), 1.98-2.16 (m, 8H, 2CH2), 2.34 (s, 3H, CH3), 5.09-5.10 (m, 2H, olefines), 5.77 (s, 1H, olefine), 5.98 (d, J=10.8 Hz, 1H, olefine), 6.21 (d, J=14.8 Hz, 1H, olefine), 6.90 (dd, J=15.2, 10.8 Hz, 1H, olefine), 9.35-11.97 (br s, 1H, COOH).
<Pharmacokinetic Tests of 1′C-Labeled Acyclic Retinoid>
The 11C-labeled acyclic retinoid 4c and the 11C-labeled peretinoin 3 synthesized by the methods described above were administered intravenously (i.v.) to Sprague-Dawley rats and cynomolgus monkeys as a PET probe, and PET images were photographed.
In a Case where the 1C-Labeled Peretinoin 3 was Administered
A PET image in a case where the 11C-labeled peretinoin 3 was administered to a rat is shown in
In a Case where 11C-Labeled Acyclic Retinoid 4c was Administered
A PET image in a case where the 11C-labeled acyclic retinoid 4c was administered to a rat is shown in
Further, from measurement of radioactivity in the brain for each time shown in
In a Case where 11C-Labeled Acyclic Retinoid 4b was Administered
A PET image in a case where the 11C-labeled acyclic retinoid 4b was administered to a rat is shown in
<Functional Effect of Peretinoin 3>
The functional effect of peretinoin 3, an acyclic retinoid that is not labeled with 11C, was evaluated.
(Inhibitory Effect on Thrombin Toxicity)
Thrombin is known to induce cerebral cortex cell death and striatal tissue atrophy. Therefore, an experiment for evaluating thrombin toxicity was performed. In other words, a coronal section having a thickness of 300 μm was prepared from a brain of a Wistar/ST rat aged 2 to 3 days after birth, a fan-shaped tissue piece including a cerebral cortex and striatum was cut out, and then left to stand on a porous membrane (Millicell-CM, Millipore Corporation), and culture was maintained at a gas-liquid interface under a conditions of 34° C./5% CO2 (medium composition: 50% minimal essential medium, 25% Hanks' balanced salt solution, 25% horse serum). On the 12th day of culture, the medium was replaced with a serum-free medium (medium composition: 75% minimal essential medium, 25% Hanks' balanced salt solution) containing 5 μg/ml propidium iodide (PI), drug treatment such as peretinoin was started, and thrombin (100 U/ml) was added to the medium 24 hours later. PI fluorescence images were acquired using a fluorescence microscope (Keyence) 72 hours after a start of thrombin treatment, and a degree of cell death was evaluated using fluorescence intensity of a cortical region as an index (sections of 100 μM NMDA treated for 72 hours were provided for each experiment, and their PI fluorescence intensity was standardized as 100%). Further, an area of a striatal region in each tissue piece was measured from bright field images of cultured tissue pieces acquired before and after the thrombin treatment, and a rate of tissue atrophy due to the thrombin treatment was quantified.
Results
The PI fluorescence images 72 hours after the start of the thrombin treatment are shown in
(Evaluation Using K252a)
K252a (3 μM) is known to inhibit TrkB, a receptor of a neurotrophic factor BDNF. Therefore, how K252a affects a cytoprotective effect of the peretinoin 3 was examined by the same method as the method of examining the effect on the thrombin toxicity.
Results
PI fluorescence images after an experiment was conducted under conditions of control, administration of only thrombin, administration of thrombin+peretinoin 3, administration of thrombin+peretinoin 3, administration of thrombin+peretinoin 3+K252a, administration of thrombin+K252a, and administration of only K252a are shown in
(Effect on Cerebral Hemorrhage Pathology)
A male ICR mouse aged 8 to 10 weeks was placed in a brain stereotaxic apparatus under triple anesthesia, and bleeding was induced by injecting 0.035 U of collagenase type VII (Sigma) into a fixed coordinate (2.3 mm lateral to a median, 0.2 mm posterior to a bregma suture, 3.5 mm below a skull) in the striatum over 2.5 minutes using a 30 gauge needle. After that, the surface of the skull was reduced with dental cement, and breeding was continued. The peretinoin 3 (40 mg/kg) was suspended in water together with 0.5% carboxymethyl cellulose, and administered intragastrically using an oral sonde three times in total 3, 27, and 51 hours after the bleeding induction. Note that for comparison, 0.5% carboxymethyl cellulose without the peretinoin 3 was also administered intragastrically in a similar manner. A degree of motor dysfunction in the mouse was evaluated by performing two types of motor function tests (beam walking test, modified limb-placing test) 6, 24, 48, and 72 hours after the bleeding induction. Further, after completion of the motor function test 72 hours later, the mouse was perfused and fixed under anesthesia, and the brain was extracted to prepare a frozen coronary section having a thickness of 16 μm. The effects of the peretinoin 3 were evaluated by identifying microglia, astrocytes, and neurons in the brain tissue, respectively, through immunohistochemistry using antibodies against Iba1, GFAP, and NeuN, and quantifying the number of cells in a given area or an immune reaction positive area.
(Results)
Changes in body weight during the test are shown in
Further, in the beam-walking test, as shown in
Furthermore, in the modified limb-placing testdeha, as shown in
From the above results, it was found that the peretinoin 3 significantly alleviated the motor dysfunction 24 to 72 hours after bleeding.
Further, results of observation of the brain tissue by immunohistochemistry after the completion of the motor function test are shown in
<Experiment Showing Differentiation Induction from Stem Cells to Nerve Cells>
The following experiment was performed to examine whether the peretinoin 3 shows the differentiation induction from stem cells to nerve cells as ATRA.
NTERA-2 cl.D1 [NT2/D1] cells (ATCC CRL-1973) (hereinafter described as “NT2 cells”) derived from human pluripotent embryonic cancer were adhesion cultured in a maintenance culture medium (high glucose D-MEM (Sigma-Aldrich, D6429), 10% FBS, 100 U/ml penicillin/streptomycin) under conditions of 37° C. and 5% CO2 for 3 to 4 days on a 10 cm plastic dish until becoming confluent. The NT2 cells were exfoliated by Accutase (Innovative Cell Technologies, #AT104), passaged, and maintenance cultured for 50 days. The medium was changed three times a week. The maintenance cultured NT2 cells were dispersed in a petri dish for low-attached microorganisms (STAR SDish9015, RIKEN) at a concentration of 1.5×106 to 2.5×106/dish, and suspension cultured on a vibrator (100 rpm) under conditions of 37° C. and 5% CO2, and after one day, 1 μM or 10 μM ATRA was added to induce differentiation and form spheroids. After 14 days, the spheroids were recovered, seeded on a 10 cm dish coated with 5 μg/ml poly-D-lysine (PDL) (Sigma-Aldrich)/laminin (LAM) (iMatrix-511, Nippi), and adhesion cultured under conditions of 37° C. and 5% CO2. From the next day, 3 types of cell division inhibitors (10 μM uridine, 10 μM floxuridine, 1 μM AraC) were added and cultured, and after 3 days, the cells were exfoliated, reseeded on a 35 mm dish coated with PDL/LAM at a concentration of 0.1×106/dish, and cultured for 4 days in the presence of 3 types of cell division inhibitors, thereby inducing differentiation into nerve cells.
After the differentiation induction, the cells were maintenance cultured in BrainPhys (trademark) Neuronal Medium (STEMCELL Technologies) containing 0.2% NeuroCult (trademark) SM1 Neuronal Supplement under conditions of 37° C. and 5% CO2 for 2 to 3 months. The medium was changed by half three times a week. Accordingly, cells positive for a nerve cell marker MAP2 were obtained and were defined as NT2-N cells.
In order to confirm a statement of synaptic receptors in mature cells, a calcium influx via NMDA-type glutamate receptors was measured as follows.
A fluorescent calcium indicator Fluo-8 AM (AAT Bioquest) was dissolved in Mg-free HHBS (Hepes-buffered Hanks' balanced salt solution (1.26 mM CaCl2, 5.33 mM KCl, 0.44 mM KH2PO4, 4.17 mM NaHCO3, 137.93 mM NaCl, 0.34 mM Na2HPO4, 5.56 mM D-glucose, 20 mM HEPES, pH 7.4)) containing 0.1 μM glycine (4 μM, 2 μM, respectively). This solution (1 ml) was loaded into NT2-N cells under conditions of 37° C. and 5% CO2 (2 hours, 30 minutes, respectively), and fluo8 was loaded into the cells. Thereafter, an extracellular fluid was replaced with Mg-free HHBS containing 0.1 μM glycine, and it was used for the following measurement after being left in an incubator (37° C., 5% CO2) of a living cell time-lapse imaging device (BioStation IM-Q, Nikon) for 1 hour or more. For the NT2-N cells placed in the device, 5 to 10 fields of view to be analyzed were manually set, and for each field of view, fluorescence intensity was continuously recorded every 2 seconds for 5 minutes at an excitation wavelength of 480 nm/a fluorescence wavelength of 520 nm. Further, after a certain period of time elapsed from the start of recording, 100 μl of the 100 μM NMDA solution (Mg-free HHBS containing 0.1 μM glycine) prepared above was administered.
A part of specimens 2 to 3 months after the start of the culture after the induction was fixed with a 4% paraformaldehyde PBS solution (at room temperature for 30 minutes). After the fixation, it was carefully washed with PBS and treated with a 1% Triton-X 100 PBS solution for 10 minutes. After the treatment, it was sufficiently washed with PBS, then blocked with a 1% BSA and 3% normal goat serum PBS solution, immersed in an anti-MAP2 antibody (1:1000, Millipore #AB 5622) and anti-Nestine antibody (1:1000, ab22035) solution, further washed, and then stained with a fluorescently labeled secondary antibody (Jackson immun laboratory, 115-545-146 and 111-585-144, 1:5000 respectively).
Results
As shown in
The present invention is not limited at all to the description of the embodiments and the examples of the invention described above. Various modifications that can be easily conceived by a person skilled in the art without departing from the scope of the claims are also included in the present invention.
The 11C-labeled cyclic retinoid of the present invention can be synthesized in a short time with a high yield, and can be suitably used as a PET probe for elucidating the intracerebral kinetics. Further, the central nervous system activator of the present invention can be used as a therapeutic agent for central nervous system degenerative diseases and a lead compound therefor.
| Number | Date | Country | Kind |
|---|---|---|---|
| 2020-193167 | Nov 2020 | JP | national |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/JP2021/042503 | 11/18/2021 | WO |
