Patent Application
20120184562
The present invention relates to 1,6 and 1,8-naphthyridines which act as inhibitors of DYRK1B and/or DYRK1A and are useful in the amelioration, treatment or control of cancer, especially solid tumors, or in the amelioration, treatment or control of Down syndrome or early onset Alzheimer's disease.
Kinases are known to be important cellular enzymes that regulate cellular functions such as regulating cell division and proliferation. WO 2008/047307. Dual-specificity tyrosine-phosphorylation-regulated kinases (DYRKs) are a subfamily of protein kinases that have dual-specificity and are believed to play roles in cell proliferation and apoptosis induction. See, e.g., Kiyotsugu Yoshida, “Role for DYRK family kinases on regulation of apoptosis,” Biochemical Pharmacology 76 (2008) pp 1389-1394; Jinghun Gao et al., “Mirk/Dyrk1B, a novel therapeutic target, mediates cells survival in non-small cell lung cancer cells,” Cancer Biology & Therapy 8:17 (2009) pp. 1671-1679. DYRK1A is believed to be implicated in neural differentiation. Yoshida, id. at 1390. Over expression of this kinase is believed to be involved in Down syndrome and Alzheimer's disease. See Nam Kim, “Putative therapeutic agents for learning and memory deficits of people with Down syndrome,” Bioorganic & Medicinal Chemistry Letters,” 16 (2006) pp 3772-76 and Joongkyu Park et al, “Function and regulation of Dyrk1A: towards understanding Down syndrome,” Cell. Mol. Life. Sci 66 (2009) pgs. 3235-3240. Thus, inhibition of this kinase is believed to be of benefit in controlling or ameliorating the effects of Down syndrome and early onset Alzheimer's disease. See, e.g., Kim, id; Park, id, and Kyung Koo et al., “QSAR analysis of pyrazolidine-3,5-diones derivatives as Dyrk1A inhibitors,” Bioorganic & Medicinal Chemistry Letters 19 (2009) pp 2324-2328.
DYRK1B (also referred to as MIRK) mediates survival and differentiation in many tissues. It is believed to be implicated in certain cancers, particularly solid tumors. See, e.g., Gao, supra (lung cancer cells); Kangmoon Lee et al, “Mirk Protein Kinase is a Mitogen-activated Protein Kinase Substrate that Mediates Survival of Colon Cancer Cells”, Cancer Research 60 (2000):3631-3637 and Xiaobing Deng et al, “The Kinase Mirk/Dyrk1B Mediates Cell Survival in a Pancreatic Ductal Adenocarcinoma,” Cancer Res 66:8 (2006) pp 4149-58 (pancreatic cancer cells). Thus, inhibition of this kinase is believed to be of benefit in controlling or ameliorating cancer. See, Cao Yang et al, “The kinase Mirk is a potential therapeutic target in osteosarcoma,” Carcinogenesis 31:4 (2010) pp 552-558 and Eileen Friedman, “The Kinase Mirk/dyrk1B: A Possible Therapeutic Target in Pancreatic Cancer,” Cancers 2 (2010) 1492-1512.
One aspect of the invention is a compound of formula I
or a pharmaceutically acceptable salt thereof, wherein X, Y and R1 through R5 are as defined below.
The present invention also relates to pharmaceutical compositions comprising one or more compounds of the invention, or a pharmaceutically acceptable salt, and a pharmaceutically acceptable carrier or excipient.
The present invention further relates to a method of treating, ameliorating or controlling cancer, including specifically solid tumors, for example lung, pancreatic, colon, breast, bone and prostate cancers in a mammal, specifically a human, comprising administering to said mammal a therapeutically effective amount of a compound according to the invention or a pharmaceutically acceptable salt thereof.
The present invention further relates to a method of treating, ameliorating or controlling Down syndrome or Alzheimer's disease in a human, comprising administering to said human a therapeutically effective amount of a compound according to the invention or a pharmaceutically acceptable salt thereof.
As used herein, the following terms shall have the following definitions.
The terms “C1-6 alkyl” or “C1-4 alkyl” refer to straight- or branched-chain saturated hydrocarbon groups having from 1 to 6, or 1 to 4, carbon atoms, respectively. Examples of C1-6 alkyl groups include, but are not limited to, methyl, ethyl, n-propyl, i-propyl, n-butyl, s-butyl, t-butyl, n-pentyl, and s-pentyl.
“Alkoxy, alkoxyl or lower alkoxy” refers to any of the above alkyl groups which is attached to the remainder of the molecule by an oxygen atom (RO—). Typical alkoxy groups include methoxy, ethoxy, isopropoxy or propoxy, butyloxy and the like. Further included within the meaning of alkoxy are multiple alkoxy side chains, e.g. ethoxy ethoxy, methoxy ethoxy, methoxy ethoxy ethoxy and the like and substituted alkoxy side chains, e.g., dimethylamino ethoxy, diethylamino ethoxy, dimethoxy-phosphoryl methoxy and the like.
“Aryl” means a substituted or unsubstituted monovalent, monocyclic or bicyclic, aromatic carboxylic hydrocarbon radical, preferably a 6-10 member aromatic ring system. Preferred aryl groups include, but are not limited to, phenyl, naphthyl, tolyl, and xylyl.
The term “cycloalkyl” as used herein means a substituted or unsubstituted stable monocyclic or polycyclic system which consists of carbon atoms only, all rings of which are saturated. Examples of cycloalkyls include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, adamantyl, cyclooctyl, bicycloalkyls, including bicyclooctanes such as [2.2.2]bicyclooctane or [3.3.0]bicyclooctane, bicyclononanes such as [4.3.0]bicyclononane, and bicyclodecanes such as [4.4.0]bicyclodecane (decalin), or spiro compounds.
“Halogen” means Cl, F and Br.
“Heteroaryl” means a substituted or unsubstituted aromatic heterocyclic ring system containing up to two rings. Preferred heteroaryl groups include, but are not limited to, thienyl (or thiophenyl), furyl, indolyl, pyrrolyl, pyridinyl, pyrazinyl, oxazolyl, thiaxolyl, quinolinyl, pyrimidinyl, imidazolyl, triazolyl and tetrazolyl.
In the case of a heteroaryl that is bicyclic it should be understood that one ring may be aryl while the other is heteroaryl and both may be independently substituted or unsubstituted.
“Hetero atom” means an atom selected from N, O and S.
“Heterocycle” or “heterocyclic ring” means a substituted or unsubstituted 5 to 10 membered, mono- or bicyclic, non-aromatic hydrocarbon, wherein 1 to 3 carbon atoms are replaced by a hetero atom selected from nitrogen, oxygen or sulfur atom. Examples include pyrrolidinyl, including pyrrolidin-1-yl, pyrrolidin-2-yl and pyrrolidin-3-yl; piperazinyl; piperidinyl; morpholinyl, including morpholin-4-yl, and the like, each of which optionally can be substituted.
In the case of a heterocycle that is bicyclic it should be understood that one ring may be heterocycle while the other is cycloalkyl, and either or both may be independently substituted. An example of such a bicyclic heterocycle is 8-oxa-3-aza-bicyclo[3.2.1]octane.
Hydroxy or hydroxyl is a prefix indicating the presence of a monovalent —O—H group.
“IC50” refers to the concentration of a particular compound required to inhibit 50% of a specific measured activity. IC50 can be measured, inter alia, as is described subsequently in Examples 102 and 103.
“Pharmaceutically acceptable,” such as pharmaceutically acceptable carrier, excipient, etc., means pharmacologically acceptable and substantially non-toxic to the subject to which the particular compound is administered.
“Pharmaceutically acceptable salt” refers to conventional acid-addition salts or base-addition salts that retain the biological effectiveness and properties of the compounds of the present invention and are formed from suitable non-toxic organic or inorganic acids or organic or inorganic bases. Sample acid-addition salts include those derived from inorganic acids such as hydrochloric acid, hydrobromic acid, hydroiodic acid, sulfuric acid, sulfamic acid, phosphoric acid and nitric acid, and those derived from organic acids such as p-toluenesulfonic acid, salicylic acid, methanesulfonic acid, oxalic acid, succinic acid, citric acid, malic acid, lactic acid, fumaric acid, trifluoroacetic acid and the like. Sample base-addition salts include those derived from ammonium, potassium, sodium and, quaternary ammonium hydroxides, such as for example, tetramethylammonium hydroxide. Chemical modification of a pharmaceutical compound (i.e. drug) into a salt is a technique well known to pharmaceutical chemists to obtain improved physical and chemical stability, hygroscopicity, flowability and solubility of compounds. See, e.g., Ansel et al., Pharmaceutical Dosage Forms and Drug Delivery Systems (1995) at pgs. 456-457.
“Substituted,” as in substituted alkyl, means that the substitution can occur at one or more positions and, unless otherwise indicated, that the substituents at each substitution site are independently selected from the specified options. The term “optionally substituted” refers to the fact that one or more hydrogen atoms of a chemical group (with one or more hydrogen atoms) can be, but does not necessarily have to be, substituted with another substituent.
In one embodiment, the present invention relates to compounds of formula I
wherein
X and Y are each independently selected from C and N, provided that when one is C and the other is N;
R1 is selected from the group
In another embodiment, the invention relates to compounds of formula Ia having the structure
wherein R1 through R4 are as defined above, or a pharmaceutically acceptable salt thereof.
In another embodiment, the invention relates to compounds of formula Ib having the structure
wherein R1 through R4 are as defined above, or a pharmaceutically acceptable salt thereof.
In another embodiment, the invention relates to compounds of formula I, including compounds of formulas Ia and Ib, wherein R1 is H, or a pharmaceutically acceptable salt thereof.
Another embodiment of the invention relates to compounds of Formula I, including compounds of formulas Ia and Ib, wherein R1 is OR6 and R6 is C1-4 alkyl. In one particular embodiment OR6 is OCH3.
Another embodiment of the invention relates to compounds of Formula I, including compounds of formulas Ia and Ib, wherein R1 is SR6 and R6 is C1-4 alkyl, or a pharmaceutically acceptable salt thereof. In one embodiment SR6 is SCH3.
Another embodiment of the invention relates to compounds of Formula I, including compounds of formulas Ia and Ib, wherein R1 is heterocycle optionally substituted with C1-4 alkyl, NR8R9 or OR8, and R8 and R9 are independently H and methyl, or a pharmaceutically acceptable salt thereof. In one embodiment R1 is heterocycle optionally substituted with CH3, NH2 or OH. In an embodiment R1 is selected from piperidinyl, piperazinyl and morpholinyl, each of which may optionally be sustituted with CH3.
Another embodiment of the invention relates to compounds of Formula I, including compounds of formulas Ia and Ib, wherein R1 is NR6R7 and R6 and R7 are independently selected from H and C1-4 alkyl that is optionally substituted with OH, OCH3, heterocycle or cycloalkyl., or a pharmaceutically acceptable salt thereof.
Another embodiment of the invention relates to compounds of Formula I, including compounds of formulas Ia and Ib, wherein R1 is as defined immediately above and R2 is OR12 and R12 is C1-4 alkyl optionally substituted with aryl or a pharmaceutically acceptable salt thereof. In one embodiment R12 is methyl. In another embodiment R12 is C1-4 substituted with phenyl which optionally is substituted with Cl.
Another embodiment of the invention relates to compounds of Formula I, including compounds of formulas Ia and Ib, wherein R1 is as defined above and R2 is NR19R11 and each of R10 and R11 are independently selected from H, and C1-6 alkyl that is optionally substituted with NH2, OH, CONH2, cycloalkyl, heterocycle, aryl, including phenyl that optionally substituted with Cl, or heteroaryl, including thiophenyl, or a pharmaceutically acceptable salt thereof. In an embodiment R10 and R11 are independently selected from H and C1-6 alkyl that is substituted with thiophene and NH2.
Another embodiment of the invention relates to compounds of Formula I, including compounds of formulas Ia and Ib, wherein R1 and R2 are as defined immediately above and R3 is Cl, or a pharmaceutically acceptable salt thereof.
Another embodiment of the invention relates to compounds of Formula I, including compounds of formulas Ia and Ib, wherein R1 and R2 are as defined immediately above and R3 is CH3, or a pharmaceutically acceptable salt thereof.
Another embodiment of the invention relates to compounds of Formula I, including compounds of formulas Ia and Ib, wherein R1, R2 and R3 are as defined immediately above and R4 is H, or a pharmaceutically acceptable salt thereof.
Another embodiment of the invention relates to compounds of Formula I, including compounds of formulas Ia and Ib, wherein R1, R2, R3 and R4 are as defined immediately above and R5 is H, or a pharmaceutically acceptable salt thereof.
Another embodiment of the invention relates to compounds of Formula I, including compounds of formulas Ia and Ib, wherein R6 and R7 are independently selected from H, C1-4 alkyl that is optionally substituted with OH or OC3 and N-ethyl, or a pharmaceutically acceptable salt thereof.
Another embodiment of the invention relates to compounds of Formula I, including compounds of formulas Ia and Ib, wherein R1, R2, R3, R4, R5, R6 and R7 are as defined immediately above and R8 and R9 are independently H or CH3, or a pharmaceutically acceptable salt thereof.
Another embodiment of the invention relates to compounds of Formula I, including compounds of formulas Ia and Ib, wherein R1, R2, R3, R4, R5, R6, R7, R8 and R9 are as defined immediately above and R10 and R11 are independently selected from H and C1-6 alkyl that is optionally substituted with aryl, including specifically phenyl that optionally is substituted with C1 or CH3, or a pharmaceutically acceptable salt thereof.
Another embodiment of the invention relates to compounds of Formula I wherein R1, R2, R3, R4, R5, R6, R7, R8 and R9 are as defined immediately above and R10 and R11 are independently selected from H and C1-6 alkyl that is optionally substituted with heteroaryl, including specifically thiophenyl, or a pharmaceutically acceptable salt thereof.
Another embodiment of the invention relates to compounds of Formula I wherein R1, R2, R3, R4, R5, R6, R7, R8 and R9 are as defined immediately above and R10 and R11 are independently selected from H and C1-4 alkyl that optionally is substituted with OH, OCH3, NH2 and CONH2, or a pharmaceutically acceptable salt thereof.
Another embodiment of the invention relates to compounds of Formula I wherein R1, R2, R3, R4, R5, R6, R7, R8 and R9 are as defined immediately above and R10 and R11 are independently selected from H and aryl, including specifically phenyl, that optionally may be substituted with Cl, or a pharmaceutically acceptable salt thereof.
Another embodiment of the invention relates to compounds of Formula I, including compounds of formulas Ia and Ib, wherein R1, R2, R3, R4, R5, R6, R7, R8, R9, and R10 and R11 are as defined immediately above and R12 is selected from C1-4 alkyl, including specifically CH3,. or a pharmaceutically acceptable salt thereof.
It is contemplated herein that salts of compounds of formula I such as hydrochloride or trifluoroacetic acid salts include salts with multiple conjugates such as mono HCl, di-HCl, etc.
Compounds according to the invention include:
The compounds of formula I, as well as their salts, that have at least one asymmetric carbon atom may be present as racemic mixtures or different stereoisomers. The various isomers can be isolated by known separation methods, e.g., chromatography.
Compounds disclosed herein and covered by formula I above may exhibit tautomerism or structural isomerism. It is intended that the invention encompasses any tautomeric or structural isomeric form of these compounds, or mixtures of such forms, and is not limited to any one tautomeric or structural isomeric form depicted in the formulas above.
The compounds of the present invention that are inhibitors of DYRK1B are useful in the treatment, amelioration or control of cell proliferative disorders, in particular chemoprevention of cancer. Chemoprevention is defined as inhibiting the development of invasive cancer by either blocking the initiating mutagenic event or by blocking the progression of pre-malignant cells that have already suffered an insult of inhibiting tumor relapse. These compounds and formulations containing said compounds are anticipated to be particularly useful in the treatment or control of solid tumors, such as, for example, lung, pancreas, colon, breast, bone and prostate tumors.
Compounds that are inhibitors of DYRK1A are useful in the treatment, amelioration or control of Down syndrome and Alzheimer's disease.
A “therapeutically effective amount” or “effective amount” of a compound in accordance with this invention means an amount of compound that is effective to alleviate, ameliorate or control symptoms of disease or prolong the survival of the subject being treated.
The therapeutically effective amount or dosage of a compound according to this invention can vary within wide limits. Such dosage will be adjusted to the individual requirements in each particular case including the specific compound(s) being administered, the route of administration, the condition being treated, as well as the patient being treated. In general, in the case of oral or parenteral administration to adult humans weighing approximately 70 Kg, a daily dosage of about 10 mg to about 10,000 mg, preferably from about 200 mg to about 1,000 mg, should be appropriate, although the upper limit may be exceeded when indicated. The daily dosage can be administered as a single dose or in divided doses, or for parenteral administration; it may be given as continuous infusion.
In an alternative embodiment, the present invention includes pharmaceutical compositions comprising at least one compound of formula I, or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable excipient and/or carrier.
These pharmaceutical compositions can be suitable for oral, nasal, topical (including buccal and sublingual), rectal, vaginal and/or parenteral administration. The formulations may conveniently be presented in unit dosage form and may be prepared by any methods well known in the art of pharmacy. The amount of active ingredient which can be combined with a carrier material to produce a single dosage form will vary depending upon the host being treated, as well as the particular mode of administration. The amount of active ingredient which can be combined with a carrier material to produce a single dosage form will generally be that amount of a formula I compound which produces a therapeutic effect. Generally, out of one hundred percent, this amount will range from about 1 percent to about ninety-nine percent of active ingredient, preferably from about 5 percent to about 70 percent, most preferably from about 10 percent to about 30 percent.
Methods of preparing these formulations or compositions include the step of bringing into association a compound of the present invention with the carrier and, optionally, one or more accessory ingredients. In general, the formulations are prepared by uniformly and intimately bringing into association a compound of the present invention with liquid carriers, or finely divided solid carriers, or both, and then, if necessary, shaping the product.
Formulations of the invention suitable for oral administration may be in the form of capsules, cachets, sachets, pills, tablets, lozenges (using a flavored basis, usually sucrose and acacia or tragacanth), powders, granules, or as a solution or a suspension in an aqueous or non-aqueous liquid, or as an oil-in-water or water-in-oil liquid emulsion, or as an elixir or syrup, or as pastilles (using an inert base, such as gelatin and glycerin, or sucrose and acacia) and/or as mouth washes and the like, each containing a predetermined amount of a compound of the present invention as an active ingredient. A compound of the present invention may also be administered as a bolus, electuary or paste.
The pharmaceutical preparations of the invention can also contain preserving agents, solubilizing agents, stabilizing agents, wetting agents, emulsifying agents, sweetening agents, coloring agents, flavoring agents, salts for varying the osmotic pressure, buffers, coating agents or antioxidants. They can also contain other therapeutically valuable substances, including additional active ingredients other than those of formula I.
The present invention also provides methods for the synthesis of the 1,6- and 1,8-naphthyridines of the invention.
The compounds of the invention can be prepared by processes known in the art. Suitable processes for synthesizing these compounds are also provided in the examples. Generally, compounds of formula I can be synthesized according to one of the below described synthetic routes.
The key transformations are coupling reactions of carbonyl and carboxy starting materials and intermediates.
The starting materials are either commercially available or can be synthesized by methods known to those of ordinary skill in the art. In general, the compound of the invention can be synthesized according to the reactions shown is Schemes 13 and 14 below. The preparation of varius reactants useful in Schemes 13 and 14 is shown in Schemes 1-12. The amino-aldehydes needed for the synthesis of the 1,6-naphthyridines of this invention can be prepared by well known methods, for example, like the steps outlined in Schemes 1-7. The amino-aldehydes needed for the synthesis of the 1,8-naphthyridines can be prepared, for example, by the methods outlined in Schemes 8-9. These amino-aldehydes can be converted to the corresponding naphthyrdine 3-carboxylic acid esters by reaction with suitable malonate derivative (for example via dialkyl malonate as in Scheme 10, or via malonic acid mono ester mono acid chloride as in Scheme 11).
In case of certain amines that contain additional functional groups, appropriate protecting groups (for example tert-butoxy-carbonyl group) may be employed to facilitate synthesis. If such protecting groups are employed, the removal of such protecting groups to generate the compounds of the invention can be accomplished by standard methods known to those skilled in the art of organic synthesis.
When the compounds of the invention are solids, it is understood by those skilled in the art that these compounds, and their salts, may exist in different crystal or polymorphic forms, all of which are intended to be within the scope of the present invention and specified formulas.
The compounds of the present invention may be synthesized according to known techniques. The following examples and references are provided to aid the understanding of the present invention. The examples are not intended, however, to limit the invention, the true scope of which is set forth in the appended claims. The names of the final products in the examples were generated using AutoNom 2000 Add-in v4.0 SP2, (function in ISIS Draw, Elsevier/MDL), or AutoNom 2000 TT v4.01.305 (Elsevier/MDL), or functions available in ChemDraw Pro Control 11.0.2 (CambridgeSoft Corp.).
Abbreviations Used in the Examples:
The following starting materials were purchased from the sources listed below:
2-Methyloxy-5-bromo pyridine Beijing Ouhe Technology Co., Ltd
3-Chloroperoxybenzic acid/Mcpba Beijing Ouhe Technology Co., Ltd
Piperidine Sinopharm Chemical Reagent Beijing Co., Ltd
Dimethyl malonate Beijing Ouhe Technology Co., Ltd
HATU Beijing Ouhe Technology Co., Ltd
n-Butyl lithium Sigma-Aldrich (Shanghai) Trading Co., Ltd
Methyl 3-amino-4-chlorobenzoate Alfa Aesar China (Tianjin) Co., Ltd
Methyl 3-aminobenzoate Alfa Aesar China (Tianjin) Co., Ltd
Methyl 3-amino-4-methylbenzoate Beijing Ouhe Technology Co., Ltd
Methyl 5-amino-2-methylbenzoate Beijing Ouhe Technology Co., Ltd
Methyl 5-amino-2-chlorobenzoate Beijing Ouhe Technology Co., Ltd
Dimethyl 1,3-acetonedicarboxylate Alfa Aesar China (Tianjin) Co., Ltd.
Triethyl orthoformate Beijing Ouhe Technology Co., Ltd
Phosphorus oxychloride Sinopharm Chemical Reagent Beijing Co., Ltd
Benzylamine Beijing Ouhe Technology Co., Ltd
1-Methylpiperazine Beijing Ouhe Technology Co., Ltd
Palladium(II) acetate Xian Kaili Technology Co., Ltd
Copper(I) iodide Alfa Aesar China (Tianjin) Co., Ltd.
L-Prolinamide Alfa Aesar China (Tianjin) Co., Ltd.
Sodium methoxide Sigma-Aldrich (Shanghai)
Lithium aluminum hydride Beijing Ouhe Technology Co., Ltd
Manganese(IV) oxide Beijing Ouhe Technology Co., Ltd
2-Amino-6-chloropyridine Oakwood
1-Methylpiperazine Aldrich
(2,2-Dimethyl-[1,3]-dioxolan-4-yl)-methylamine Aldrich
Dimethyl malonate Aldrich
HATU Chem-Impex
Methyl 3-aminobenzoate Alfa
Methyl 3-amino-4-methylbenzoate Alfa
Methyl 3-amino-4-chlorobenzoate TCI-US
Methyl 5-amino-2-methylbenzoate AKSCI
Methyl-5-amino-2-chlorobenzoate Oakwood
Sodium hexamethyldisilazane Alfa
(2-Amino-2-phenyl-ethyl)-carbamic acid tert-butyl ester was prepared according to the literature procedure of Seefeld, M. A.; Rouse, M. B.; Heerding, D. A.; Peace, S.; Yamashita, D. S.; McNulty, K. C. WO 2008/098104, Aug. 14, 2008.
To a stirred solution of 2-amino-1-phenylethanol (20 g, 145.8 mmol) in THF (300 mL) was added the solution of Boc2O (31.1 g, 153.1 mmol) in THF (100 mL) at 0° C. After addition, the mixture was stirred at room temperature for 0.5 hour. This mixture was concentrated to give the pure (2-hydroxy-2-phenyl-ethyl)-carbamic acid tert-butyl ester as a white solid. (Yield 34.4 g, 100%).
To a solution of (2-hydroxy-2-phenyl-ethyl)-carbamic acid tert-butyl ester (34.4 g, 145.0 mmol), phthalimide (21.3 g, 145 mmol), and PPh3 (49.4 g, 188.5 mmol) was added drop-wise DEAD (32.8 g, 188.5 mmol) under stirring at 0° C. After addition, the mixture was stirred at room temperature for an additional 1 hour. The mixture was concentrated under reduced pressure. The residue was purified by column chromatography on silica gel (petroleum ether:ethyl acetate, 20:1 to 5:1) to give [2-(1,3-dioxo-1,3-dihydro-isoindol-2-yl)-2-phenyl-ethyl]-carbamic acid tert-butyl ester as a white solid. (Yield 39 g, 74%).
1H NMR (300 MHz, CDCl3): δ 7.88-7.80 (m, 2H), 7.74-7.68 (m, 2H), 7.49-7.47 (m, 2H), 7.38-7.26 (m, 3H), 5.56-5.50 (m, 1H), 4.83 (brs, 1H), 4.28-4.22 (m, 1H), 3.93-3.87 (m, 1H), 1.35 (s, 9H). LC-MS: [M-Boc+H]+ 267.
To a solution of [2-(1,3-dioxo-1,3-dihydro-isoindol-2-yl)-2-phenyl-ethyl]-carbamic acid tert-butyl ester (23 g, 63 mmol) in THF (180 mL) and MeOH (180 mL) was added 85% hydrazine hydrate (37 mL, 630 mmol) slowly. The resulting mixture was heated to 65° C. for 15 hours. The reaction mixture was cooled to room temperature, then concentrated to dryness. The residue was purified by column chromatography on silica gel (dichloromethane:MeOH, 100:1, 1% NH3H2O) to give (2-amino-2-phenyl-ethyl)-carbamic acid tert-butyl ester as a white solid. (Yield 7.4 g, 50%).
1H NMR (300 MHz, CDCl3): δ 7.35-7.24 (m, 5H), 4.81 (brs, 1H), 4.08-4.03 (m, 1H), 3.38-3.21 (m, 2H), 1.44 (s, 9H). LC-MS: [M+H]+ 237.
(3-Amino-3-phenyl-propyl)-carbamic acid tert-butyl ester was prepared according to the literature procedure of Seefeld, M. A.; Rouse, M. B.; Heerding, D. A.; Peace, S.; Yamashita, D. S.; McNulty, K. C. WO 2008/098104, Aug. 14, 2008.
To a stirred suspension of LAH (20 g, 517 mmol) in dry THF (500 mL) was added a solution of 3-oxo-3-phenylpropanenitrile (30 g, 207 mmol) in dry THF (300 mL) drop-wise at 0° C. under nitrogen atmosphere. The mixture was warmed to 25° C. and then heated at 70° C. for 2 hours. After cooling to 0° C., a saturated solution of sodium hydroxide was added drop-wise and extracted with dichloromethane (200 mL). The organic solution was dried over anhydrous sodium sulfate and concentrated to dryness. The residue was purified by column chromatography (methanol:dichloromethane, 1:10) to afford 3-amino-1-phenyl-propan-1-ol. (Yield 30 g, crude).
LC-MS: [M+H]+ 152.
Et3N (1.36 g, 14 mmol) was added to a solution of 3-amino-1-phenyl-propan-1-ol (1.7 g, 11.3 mmol) in THF (20 mL) under stirring. Boc2O (3.0 g, 13.7 mmol) in THF (20 mL) was added dropwise to the solution at 0° C. Then the resulting mixture was warmed to room temperature and stirred for an additional 2 hours. The mixture was concentrated in vacuo. The residue was purified by column chromatography on silica gel (petroleum ether:ethyl acetate, 3:1) to give (3-hydroxy-3-phenyl-propyl)-carbamic acid tert-butyl ester. (Yield 1.7 g, 60%).
LC-MS: [M+Na]+ 274.
To a solution of (3-hydroxy-3-phenyl-propyl)-carbamic acid tert-butyl ester (10.4 g, 41.4 mmol), phthalimide (5.2 g, 36.6 mmol), and PPh3 (14.6 g, 55.5 mmol) in THF (204 mL) was added dropwise DEAD (8.9 mL, 55 mmol) with stirring at 0° C. Then the resulting mixture was warmed to room temperature for an additional 2 hours. The mixture was concentrated in vacuo. The residue was purified by column chromatography on silica gel (petroleum ether:ethyl acetate, 3:1) to give [3-(1,3-dioxo-1,3-dihydro-isoindol-2-yl)-3-phenyl-propyl]-carbamic acid tert-butyl ester. (Yield 10.5 g, 66.8%).
1H NMR (300 MHz, CDCl3): δ 7.81-7.75 (m, 2H), 7.69-7.64 (m, 2H), 7.53-7.50 (m, 2H), 7.34-7.23 (m, 3H), 5.44-5.38 (m, 1H), 4.74 (brs, 1H), 3.29-3.07 (m, 2H), 2.83-2.75 (m, 1H), 2.51-2.42 (m, 1H), 1.42 (s, 9H). LC-MS: [M-Boc+H]+ , 281.
85% Hydrazine hydrate (5.1 mL, 74 mmol) was added to a solution of [3-(1,3-dioxo-1,3-dihydro-isoindol-2-yl)-3-phenyl-propyl]-carbamic acid tert-butyl ester (2.8 g, 7.4 mmol) in THF (25 mL) and MeOH (25 mL). The resulting mixture was heated to 65° C. for 6 hours. Then the precipitate was filtered, and the filtrate was concentrated in vacuo to give crude product which was purified by column chromatography on silica gel (dichloromethane:MeOH, 100:1, 1% NH3H2O) to give (3-amino-3-phenyl-propyl)-carbamic acid tert-butyl ester as an off-white solid. (Yield 1.7 g, 92%).
1H NMR (300 MHz, CDCl3): δ 7.31-7.18 (m, 5H), 6.82 (brs, 1H), 3.78-3.74 (m, 1H), 2.92 (brs, 2H), 1.82 (s, 2H), 1.63-1.61 (m, 2H), 1.37 (s, 9H). LC-MS: [M+H]+ 251.
[2-Amino-2-(3-chloro-phenyl)-ethyl]-carbamic acid tert-butyl ester was prepared in an analogous process according to the literature procedure of Seefeld, M. A.; Rouse, M. B.; Heerding, D. A.; Peace, S.; Yamashita, D. S.; McNulty, K. C. WO 2008/098104, Aug. 14, 2008.
To a stirred suspension of KCN (5.04 g, 78 mmol) in methanol (20 mL) was added 3-chlorobenzaldehyde (7.0 g, 50 mmol) at 0° C. under nitrogen atmosphere. Then acetic acid (4.4 mL) was added dropwise at 0° C. After 30 minutes, the mixture was warmed to 15° C. and stirred for 5 hours. Then the reaction mixture was concentrated to dryness and extracted with ethyl acetate (200 mL). The organic solution was washed with water (3×25 mL), brine (25 mL), dried over anhydrous sodium sulfate and concentrated to dryness. The resulting residue was purified by column chromatography (ethyl acetate:petroleum ether, 1:15) to afford (3-chloro-phenyl)-hydroxy-acetonitrile. (Yield 8.2 g, 97%).
LC-MS: [M+Na]+ 190.
To a stirred suspension of LAH (2.36 g, 59 mmol) in dry THF (70 mL) was added a solution of (3-chloro-phenyl)-hydroxy-acetonitrile (4.0 g, 24 mmol) in dry THF (55 mL) dropwise at 0° C. under nitrogen atmosphere. The mixture was warmed to 25° C. and then heated at 60° C. for 2 hours. After cooling to 0° C., a saturated solution of sodium hydroxide was added dropwise and extracted with dichloromethane (200 mL). The organic solution was dried over anhydrous sodium sulfate and concentrated to dryness. The residue was purified by column chromatography (methanol:dichloromethane, 1:10) to afford 2-amino-1-(3-chloro-phenyl)-ethanol. (Yield 2.86 g, 70%).
LC-MS: [M+H]+ 172.
To a stirred solution of 2-amino-1-(3-chloro-phenyl)-ethanol (2.86 g, 16.7 mmol) in THF (100 mL) was added Boc2O (4.3 g, 20 mmol). After 1 hour, the mixture was concentrated to dryness. The residue was purified by column chromatography (methanol:dichloromethane, 1:100) to afford [2-(3-chloro-phenyl)-2-hydroxy-ethyl]-carbamic acid tert-butyl ester. (Yield 3.9 g, 72%).
LC-MS: [M+Na]+ 294.
To a stirred solution of [2-(3-chloro-phenyl)-2-hydroxy-ethyl]-carbamic acid tert-butyl ester (20 g, 73.5 mmol), phthalimide (11.1 g, 73.5 mmol) and PPh3 (25.1 g, 95.5 mmol) in THF (500 mL) was added DEAD (11.4 mL, 95.5 mmol) dropwise at −5 to 0° C. The reaction mixture was stirred at room temperature for 3 hours. Then the mixture was concentrated to dryness. The residue was purified by column chromatography (ethyl acetate:petroleum ether, 1:10) to afford [2-(3-chloro-phenyl)-2-(1,3-dioxo-1,3-dihydro-isoindol-2-yl)-ethyl]-carbamic acid tert-butyl ester. (Yield 20 g, 69%).
LC-MS: [M+H]+ 401.
To a stirred solution of [2-(3-chloro-phenyl)-2-(1,3-dioxo-1,3-dihydro-isoindol-2-yl)-ethyl]-carbamic acid tert-butyl ester (2.5 g, 62 mmol) in THF (10 mL) and methanol (10 mL) was added hydrazine hydrate (3.1 g, 62 mmol). The mixture was heated to 55° C. for 1 hour. Then it was concentrated to dryness, dissolved in H2O (5 mL) and extracted with ethyl acetate (50 mL). The organic mixture was concentrated and purified by column chromatography (methanol:dichloromethane, 1:100) to afford [2-amino-2-(3-chloro-phenyl)-ethyl]-carbamic acid tert-butyl ester. (Yield 1.325 g, 79%).
LC-MS: [M+H]+ 271.
(3-Amino-3-thiophen-3-yl-propyl)-carbamic acid tert-butyl ester was prepared in an analogous process according to the literature procedure of Seefeld, M. A.; Rouse, M. B.; Heerding, D. A.; Peace, S.; Yamashita, D. S.; McNulty, K. C. WO 2008/098104, Aug. 14, 2008.
To a stirred suspension of LAH (1.45 g, 38.1 mmol) in dry THF (120 mL) was added a solution of 3-oxo-3-(thiophen-3-yl)propanenitrile (4.8 g, 31.8 mmol) in dry THF (40 mL) dropwise at 0° C. under nitrogen atmosphere. The mixture was warmed to 25° C. and then heated at 65° C. for 6 hours. After cooling to 0° C., a saturated solution of sodium hydroxide (2 mL) was added dropwise and the mixture was filtered. The filtrate was concentrated to dryness to give crude 3-amino-1-thiophen-3-yl-propan-1-ol which was used in next step without further purification. 1H NMR (300 MHz, CDCl3): δ 7.29-7.26 (m, 2H), 7.05 (dd, 1H, J1=4.8 Hz, J2=1.2 Hz), 5.04 (dd, 1H, J1=8.1 Hz, J2=3.0 Hz), 3.10-3.05 (m, 2H), 1.82-1.77 (m, 2H).
To a stirred solution of crude 3-amino-1-thiophen-3-yl-propan-1-ol (23 g) in THF (100 mL) was added Boc2O (31.6 g, 146.3 mmol). The mixture was stirred at room temperature for 1 hour and then concentrated to dryness. The residue was purified by column chromatography (ethyl acetate:petroleum ether, 1:10) to afford (3-hydroxy-3-thiophen-3-yl-propyl)-carbamic acid tert-butyl ester. (Yield 21.5 g, 51% for two steps).
1H NMR (300 MHz, CDCl3): δ 8.08-8.06 (m, 1H), 7.55-7.53 (m, 1H), 7.34-7.30 (m, 1H), 5.10 (s, 1H), 3.52-3.48 (m, 2H), 3.13-3.09 (m, 2H), 1.42 (s, 9H). LC-MS: [M+Na]+ 280.
To a stirred solution of (3-hydroxy-3-thiophen-3-yl-propyl)-carbamic acid tert-butyl ester (21.5 g, 83.6 mmol), phthalimide (12.3 g, 83.6 mmol), and PPh3 (28.5 g, 108.6 mmol) in THF (400 mL) was added DEAD (17.6 mL, 108.6 mmol) dropwise at 25° C. The mixture was stirred at room temperature for 14 hours, then concentrated. The residue was purified by column chromatography (ethyl acetate:petroleum ether, 1:6) to afford 3-(1,3-dioxo-1,3-dihydro-isoindol-2-yl)-3-thiophen-3-yl-propyl]-carbamic acid tert-butyl ester. (Yield 12 g, 38%).
1H NMR (300 MHz, CDCl3): δ 7.82-7.77 (m, 2H), 7.72-7.68 (m, 2H), 7.36 (d, 1H, J=1.8 Hz), 7.26-7.18 (m, 2H), 5.50 (dd, 1H, J1=9.6 Hz, J2=6 Hz), 4.65 (brs, 1H), 3.24-3.07 (m, 2H), 2.72-2.67 (m, 1H), 2.47-2.40 (m, 1H), 1.40 (s, 9H). LC-MS: [M+H-Boc]+ 287.
To a stirred solution of 3-(1,3-dioxo-1,3-dihydro-isoindol-2-yl)-3-thiophen-3-yl-propyl]-carbamic acid tert-butyl ester (12 g, 31.1 mmol) in methanol (150 mL) was added hydrazine hydrate (18 mL, 85% aqueous). The mixture was heated to reflux for 14 hours. After cooling to room temperature, the reaction mixture was filtered. The filtrate was concentrated and the residue was purified by column chromatography (methanol:dichloromethane, 1:50 to 1:20, 0.1% NH3H2O) to afford (3-amino-3-thiophen-3-yl-propyl)-carbamic acid tert-butyl ester. (Yield 7.6 g, 95%).
1H NMR (300 MHz, CDCl3): δ 7.49 (s, 1H), 7.25-7.08 (m, 2H), 6.82 (brs, 1H), 3.85 (t, 1H, J=6.0 Hz), 3.18-2.95 (m, 4H), 1.75-1.62 (m, 2H), 1.37 (s, 9H). LC-MS: [M+H]+ 257.
(2-Amino-2-thiophen-3-yl-ethyl)-carbamic acid tert-butyl ester was prepared in an analogous process according to the literature procedure of Seefeld, M. A.; Rouse, M. B.; Heerding, D. A.; Peace, S.; Yamashita, D. S.; McNulty, K. C. WO 2008/098104, Aug. 14, 2008.
To a stirred suspension of KCN (18.6 g, 286 mmol) in methanol (100 mL) was added thiophene-3-carbaldehyde (20 mL, 178 mmol) at 0° C. under nitrogen atmosphere. Then acetic acid (4.4 mL) was added dropwise at 0° C. After 30 minutes, the mixture was warmed to 15° C. and stirred for 20 hours. NaHCO3 (15 g) was added. The mixture was concentrated and extracted with ethyl acetate (200 mL). The organic mixture was washed with water (3×25 mL), brine (25 mL), dried over anhydrous sodium sulfate and concentrated to dryness. The residue was purified by column chromatography (ethyl acetate:petroleum ether, 1:10) to afford hydroxy-thiophen-3-yl-acetonitrile. (Yield 15 g, 60%).
LC-MS: [M+Na]+ 162.
To a stirred suspension of LAH (8.7 g, 225 mmol) in dry THF (300 mL) was added a solution of hydroxy-thiophen-3-yl-acetonitrile (12.5 mL, 90 mmol) in dry THF (50 mL) dropwise at 0° C. under nitrogen atmosphere. Then the mixture was warmed to 25° C. and stirred overnight. After cooling to 10° C., H2O (8.7 mL) was added to the solution, followed by NaOH solution (8.7 mL, 15%), then H2O (26 mL). The reaction mixture was filtered and the filtration was concentrated to dryness to afford crude 2-amino-1-thiophen-3-yl-ethanol. (Yield 12.9 g, crude). LC-MS: [M+H]+ 144.
To a stirred solution of crude 2-amino-1-thiophen-3-yl-ethanol (12.9 g, crude) in THF (150 mL) was added Boc2O (21.6 g, 99 mmol). After stirring for 1 hour, the mixture was concentrated to dryness which was purified by column chromatography (ethyl acetate:petroleum ether, 1:5) to afford (2-hydroxy-2-thiophen-3-yl-ethyl)-carbamic acid tert-butyl ester. (Yield 15.3 g, 70%).
LC-MS: [M+Na]+ 266.
To a stirred solution of (2-hydroxy-2-thiophen-3-yl-ethyl)-carbamic acid tert-butyl ester (15.3 g, 63 mmol), pathalimide (9.5 g, 63 mmol), PPh3 (21.4 g, 82 mmol) in THF (400 mL) was added DEAD (12.6 mL, 82 mmol) dropwise at 25° C. After 20 hours, the mixture was concentrated to dryness. The residue was purified by column chromatography (ethyl acetate:petroleum ether, 1:6) to afford [2-(1,3-dioxo-1,3-dihydro-isoindol-2-yl)-2-thiophen-3-yl-ethyl]-carbamic acid tert-butyl ester. (Yield 23 g, crude).
LC-MS: [M+Na]+ 395.
To a stirred solution of [2-(1,3-dioxo-1,3-dihydro-isoindol-2-yl)-2-thiophen-3-yl-ethyl]-carbamic acid tert-butyl ester (23 g, crude) in THF (100 mL) and methanol (100 mL) was added hydrazine hydrate (63 g, 1.26 mol). The mixture was heated to 60° C. for 2 hours and then cooled to 20° C. The reaction mixture was filtered and the filtration was concentrated to dryness. The residue was purified by column chromatography (methanol:dichloromethane, 1:50) to afford (2-amino-2-thiophen-3-yl-ethyl)-carbamic acid tert-butyl ester. (Yield 8.6 g, 57% for the two steps).
LC-MS: [M+H]+ 243.
[3-Amino-3-(3-chloro-phenyl)-propyl]-carbamic acid tert-butyl ester was prepared in an analogous process according to the literature procedure of Seefeld, M. A.; Rouse, M. B.; Heerding, D. A.; Peace, S.; Yamashita, D. S.; McNulty, K. C. WO 2008/098104, Aug. 14, 2008.
To a stirred suspension of LAH (16 g, 90 mmol) in dry THF (200 mL) was added a solution of 3-(3-chlorophenyl)-3-oxopropanenitrile (10.4 g, 270 mmol) in dry THF (200 mL) dropwise at 0° C. under nitrogen atmosphere. The mixture was warmed to 25° C. and then heated at 60° C. for 3 hours. After cooling to 0° C., a saturated solution of sodium hydroxide was added dropwise and extracted with ethyl acetate (200 mL). The solution was dried over anhydrous sodium sulfate and concentrated to dryness. The crude 3-amino-1-(3-chloro-phenyl)-propan-1-ol obtained was used in the next step without further purification. (Yield 14.5 g, crude).
LC-MS: [M+H]+ 186.
To a stirred solution of crude 3-amino-1-(3-chloro-phenyl)-propan-1-ol (29 g, 156 mmol) in THF (300 mL) was added Boc2O (40.5 g, 187 mmol). After 0.5 hour, the mixture was concentrated to dryness. The residue was purified by column chromatography (ethyl acetate:petroleum ether, 1:20) to afford [3-(3-chloro-phenyl)-3-hydroxy-propyl]-carbamic acid tert-butyl ester. (Yield 23 g, 52%).
LC-MS: [M+Na]+ 308.
To a stirred solution of [3-(3-chloro-phenyl)-3-hydroxy-propyl]-carbamic acid tert-butyl ester (12 g, 42 mmol), phthalimide (6.2 g, 42 mmol), and PPh3 (14.3 g, 55 mmol) in THF (150 mL) was added DEAD (9.0 mL, 55 mmol) dropwise at about 5° C. After 1 hour, the mixture was concentrated to dryness. The residue was purified by column chromatography (ethyl acetate:petroleum ether, 1:8) to afford [3-(3-chloro-phenyl)-3-(1,3-dioxo-1,3-dihydro-isoindol-2-yl)-propyl]-carbamic acid tert-butyl ester. (Yield 15.65 g, 90%).
LC-MS: [M+H]+ 415.
To a stirred solution of [3-(3-chloro-phenyl)-3-(1,3-dioxo-1,3-dihydro-isoindol-2-yl)-propyl]-carbamic acid tert-butyl ester (0.15 g, 0.36 mmol) in THF (2 mL) and methanol (2 mL) was added hydrazine hydrate (0.18 g, 3.6 mmol). The mixture was heated to 55° C. for 2 hours. Then the reaction mixture was concentrated and extracted with ethyl acetate (10 mL). The organic mixture was washed with water (3×1 mL), brine (1 mL), dried over anhydrous sodium sulfate and concentrated to dryness. The residue was purified by column chromatography (methanol:dichloromethane, 1:100) to afford [3-amino-3-(3-chloro-phenyl)-propyl]-carbamic acid tert-butyl ester. (Yield 0.061 g, 60%).
LC-MS: [M+H]+ 285.
3-Oxo-pentanedioic acid dimethyl ester (100 g, 575 mmol), (EtO)3CH (95 mL, 575 mmol), Ac2O (108 mL) and HOAc (108 mL) were mixed and heated at 120° C. for 1.5 hours, then allowed to cool to 25° C. The volatiles were removed under reduced pressure while maintaining the water bath around 85° C. To the crude liquid was added aqueous NH3 (25%, 150 mL) in portions with swirling in an ice bath and the stirring was continued for 1 hour. The mixture was acidified by the addition of 6 N HCl. The yellow precipitate was collected by filtration, boiled in toluene, cooled and filtered to give 4,6-dihydroxy-nicotinic acid methyl ester as an orange solid. (Yield 65 g, 67%).
1H NMR (300 MHz, d6-DMSO): δ 8.06 (s, 1H), 5.65 (s, 1H), 3.83 (s, 3H). LC-MS: [M+H]+ 170.
4,6-Dihydroxy-nicotinic acid methyl ester (3.9 g, 23 mmol) (from Example 7 supra) was added to POCl3 (20 mL) at 0° C., then Et3N (5 mL) was added to the mixture slowly. After addition, the mixture was heated at 120° C. for 2 hours, then allowed to cool to 0° C. The reaction solution was poured slowly and portion-wise into ice-water (100 mL). Solid K2CO3 was added to adjust pH to 8. The resulting precipitate was collected by filtration. The solid was dissolved in ethyl acetate (50 mL), dried over anhydrous Na2SO4, filtered and concentrated to afford 4,6-dichloro-nicotinic acid methyl ester. (Yield 4.6 g, 97.2%).
1H NMR (301 MHz, CDCl3) δ 8.85 (s, 1H), 7.47 (s, 1H), 3.97 (s, 3H). LC-MS: [M+H]+ 205.9.
The mixture of 4,6-dichloro-nicotinic acid methyl ester (4.6 g, 22.3 mmol) (from Example 8 supra), benzylamine (2.63 g, 24.5 mmol) and Et3N (6.75 g, 66.9 mmol) in DMF (10 mL) was stirred at room temperature for 3 hours. The reaction mixture was poured into water (250 mL) and extracted with ethyl acetate (3×200 mL). Combined organic layers was washed with water (100 mL), brine (100 mL), dried over anhydrous sodium sulfate, filtered and concentrated. The residue was purified by silica gel chromatography (eluting with petroleum ether/ethyl acetate, 20:1) to give 4-benzylamino-6-chloro-nicotinic acid methyl ester. (Yield 5.5 g, 89.1%).
1H NMR (300 MHz, CDCl3): δ 8.70 (s, 1H), 8.53 (s, 1H), 7.38-7.30 (m, 5H), 6.56 (s, 1H), 4.44 (d, 2H, J=5.6 Hz), 3.90 (s, 3H). LC-MS: [M+H]+ 277.
A mixture of 4-benzylamino-6-chloro-nicotinic acid methyl ester (10.5 g, 38.04 mmol) (from Example 9 supra) and sodium methoxide (20.5 g, 380.43 mmol) in DMSO (120 mL) was heated at 135° C. for 3 hours. TLC showed that compound 4-benzylamino-6-chloro-nicotinic acid methyl ester was consumed completely. It was cooled to room temperature, diluted with water (500 mL), extracted with dichloromethane (200 mL). The aqueous phase was acidified with 1 N HCl to pH 3-4. The precipitate was collected by filtration, washed with water, dried under reduced pressure to give 4-benzylamino-6-methoxy-nicotinic acid as a white powder. (Yield 8.3 g, 84%).
1H NMR (300 MHz, d6-DMSO) δ 8.48 (s, 2H), 7.30 (dt, 5H, J=6.4, 4.2 Hz), 5.86 (s, 1H), 4.46 (d, 2H, J=5.8 Hz), 3.77 (s, 3H). LC-MS: [M+H]+ 259.
4-Benzylamino-6-methoxy-nicotinic acid (7.3 g, 28.29 mmol) (from Example 10 supra) was dissolved in conc. H2SO4 (37 mL) and the resulting solution was stirred at room temperature for 4 hours. Then it was added into MeOH (200 mL) at 0° C. and the solution was heated at reflux overnight. It was then cooled to 0° C., basified with solid NaHCO3 and saturated aqueous NaHCO3 (about 500 mL), extracted with dichloromethane (3×300 mL), washed with water (2×200 mL) and brine (200 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure to give 4-amino-6-methoxy-nicotinic acid methyl ester as a white powder. (Yield 4.24 g, 82.8%).
1H NMR (300 MHz, CDCl3) δ 8.63 (s, 1H), 6.03 (s, 2H), 5.88 (s, 1H), 3.92 (s, 3H), 3.87 (s, 3H).
LC-MS: [M+H]+ 183.
To a suspension of LiAlH4 (2.57 g, 67.58 mmol) in THF (30 mL) was added a solution of 4-amino-6-methoxy-nicotinic acid methyl ester (4.92 g, 27.03 mmol) (from Example 11 supra) in THF (45 mL) at 10° C. under N2. The resulting reaction mixture was warmed up to room temperature and stirred for 3 hours. TLC showed that little 4-amino-6-methoxy-nicotinic acid methyl ester remained in the reaction mixture. Then it was cooled to 0° C. and water (15 mL) was added dropwise. A lot of white solid was formed. The mixture was warmed to room temperature and stirred for 20 minutes. Then it was diluted with THF (100 mL), filtered and the filter cake was washed with THF. The filtrate was concentrated and co-evaporated with EtOH to give (4-amino-6-methoxy-pyridin-3-yl)-methanol as an off-white solid. (Yield 3.78 g, 91%). 1H NMR (300 MHz, d6-DMSO) δ 7.62 (s, 1H), 5.90 (s, 1H), 5.74 (s, 2H), 4.91 (t, 1H, J=5.4 Hz), 4.34 (d, 2H, J=5.4 Hz), 3.72 (s, 3H). LC-MS: [M+H]+ 155.
To a solution of (4-amino-6-methoxy-pyridin-3-yl)-methanol (3.78 g, 24.55 mmol) (from Example 12 supra) in THF (100 mL) was added MnO2 (21.34 g, 245.5 mmol) at room temperature. The resulting reaction mixture was stirred overnight under N2. TLC showed that little (4-amino-6-methoxy-pyridin-3-yl)-methanol remained in the reaction mixture. It was filtered and the filter cake was washed with THF. The filtrate was concentrated under reduced pressure to dryness to give 4-amino-6-methoxy-pyridine-3-carbaldehyde as a yellow solid. (Yield 3.73 g, 100%).
1H NMR (300 MHz, d6-DMSO) δ 9.76 (s, 1H), 8.31 (s, 1H), 7.43 (s, 2H), 5.95 (s, 1H), 3.83 (s, 3H). LC-MS: [M+H]+ 153.
To a solution of 4-amino-6-methoxy-pyridine-3-carbaldehyde (3.668 g, 24.13 mmol) (from Example 13 supra) in MeOH (460 mL) was added CH2(CO2CH3)2 (8.82 g, 66.84 mmol), AcOH (145 mg, 2.41 mmol) and piperidine (5.74 g, 67.56 mmol) at room temperature. The resulting reaction mixture was heated to 60° C. for 18 hours under N2. LC-MS showed that little 4-amino-6-methoxy-pyridine-3-carbaldehyde existed in the reaction mixture. Then it was cooled to room temperature and concentrated under reduced pressure. The residue was washed with ether (100 mL) and the precipitate was collected by filtration, dried under reduced pressure to give 7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carboxylic acid methyl ester as a white solid. (Yield 5.1 g, 90%).
1H NMR (300 MHz, d6-DMSO) δ 12.03 (s, 1H), 8.75 (s, 1H), 8.61 (s, 1H), 6.52 (s, 1H), 3.95 (s, 3H), 3.83 (s, 3H). LC-MS: [M+H]+ 235.
A mixture of 7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carboxylic acid methyl ester (5.1 g, 21.79 mmol) (from Example 14 supra), NaOH (2.62 g, 65.38 mmol) in MeOH (70 mL) and water (70 mL) was heated at 65° C. for 18 hours. A lot of white solid was formed during reaction. Then the reaction mixture was cooled to room temperature, diluted with water and concentrated under reduced pressure to remove most of the MeOH. The resulting mixture was acidified with 1 N aqueous HCl to ph 3-4. White precipitate formed was collected by filtration, washed with water and dried under reduced pressure to give 7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carboxylic acid as a white solid. (Yield 4.7 g, 98%).
1H NMR (300 MHz, d6-DMSO) δ 13.41 (s, 2H), 8.92 (s, 2H), 6.61 (s, 1H), 3.96 (s, 3H). LC-MS: [M+H]+ 221.
The mixture of 4-benzylamino-6-chloro-nicotinic acid methyl ester (14.46 g, 52.39. mmol) (from Example 9 supra), 1-methylpiperazine (9.43 g, 94.3 mmol), Pd(OAc)2 (868 mg), CuI (868 mg), K2CO3 (18 g, 130.53 mmol), L-proline (868 mg) in 1-methylpyrrolidin-2-one (30 mL) was stirred at 100° C. for 36 hours. It was diluted with water and extracted with CH2Cl2 (3×50 mL). After the solvent was removed, the residue was purified by flash chromatography over silica gel (eluting with CH2Cl2/MeOH, 40/1 to 8/1) to give 4-benzylamino-6-(4-methyl-piperazin-1-yl)-nicotinic acid methyl ester (14.2 g, 80%). LC-MS: [M+H]+ 341.
4-Benzylamino-6-(4-methyl-piperazin-1-yl)-nicotinic acid methyl ester (3.47 g, 10.2 mmol) (from Example 16 supra) was dissolved in THF (20 mL). LiAlH4 (0.7 g, 18.36 mmol) was added to the mixture in small batches at 0° C. After addition, the mixture was stirred at room temperature under nitrogen overnight. At that time, Na2SO4.10H2O (1.4 g) was added and the mixture was filtered and washed with THF (500 mL). The filtrate was concentrated to give [4-benzylamino-6-(4-methyl-piperazin-1-yl)-pyridin-3-yl]-methanol as white solid. (Yield 3.22 g, 100%).
1H NMR (300 MHz, d6-DMSO): δ 7.59 (s, 1H), 7.39-7.24 (m, 5H), 6.23 (t, 1H, J=5.4 Hz), 5.79 (s, 2H), 4.93 (t, 1H, J=5.4 Hz), 4.40-4.37 (m, 5H), 3.34-3.29 (m, 4H), 2.17 (s, 3H). LC-MS: [M+H]+ 313.
The mixture of [4-benzylamino-6-(4-methyl-piperazin-1-yl)-pyridin-3-yl]-methanol (3.22 g, 10.31 mmol) (from Example 17 supra), ammonium formate (3.25 g, 51.54 mmol), 10% Pd/C (3.22 g) in MeOH (30 mL) was heated at 80° C. for 16 hours. The mixture was filtered and the filtrate was concentrated and purified by silica gel chromatography, eluting with CH2Cl2/MeOH/NH3.H2O=300/5/1.5 to 300/20/1.5 V/V/V to give [4-amino-6-(4-methyl-piperazin-1-yl)-pyridin-3-yl]-methanol. (Yield 206 mg, 9%).
1H NMR (300 MHz, d6-DMSO): δ 7.59 (s, 1H), 5.93 (s, 1H), 5.50 (s, 2H), 4.28 (d, 2H, J=5.4 Hz), 3.32-3.29 (m, 2H), 2.36-2.33 (m, 4H), 2.19 (s, 3H). LC-MS: [M+H]+ 223.
The mixture of [4-amino-6-(4-methyl-piperazin-1-yl)-pyridin-3-yl]-methanol (206 mg, 0.93 mmol) (from Example 18 supra), MnO2 (807 mg, 9.28 mmol) in CH2Cl2 (20 mL) was stirred at room temperature overnight. Then the mixture was filtered. The filtrate was concentrated to give 4-amino-6-(4-methyl-piperazin-1-yl)-pyridine-3-carbaldehyde which was used in the next reaction without purification. (Yield 180 mg, 88%).
LC-MS: [M+H]+ 221.
The mixture of 4-amino-6-(4-methyl-piperazin-1-yl)-pyridine-3-carbaldehyde (180 mg, 0.82 mmol) (from Example 19 supra), CH2(COOMe)2 (300 mg, 2.27 mmol), AcOH (5 mg, 0.082 mmol) and piperidine (195 mg, 2.29 mmol) in MeOH (20 mL) was heated at 60° C. for 18 hours. After cooling, the mixture was concentrated to give 7-(4-methyl-piperazin-1-yl)-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carboxylic acid methyl ester which was used without further purification. (Yield 247 mg, 100%).
1H NMR (300 MHz, d6-DMSO): δ 8.60 (s, 1H), 8.46 (s, 1H), 6.29 (s, 1H), 3.57 (t, 4H, J=4.2 Hz), 2.75 (brs, 3H), 2.40 (t, 4H, J=4.5 Hz), 2.22 (s, 3H,). LC-MS: [M+H]+ 302.9.
A mixture of 7-(4-methyl-piperazin-1-yl)-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carboxylic acid methyl ester (232 mg, 0.77 mmol) (from Example 20 supra), NaOH (92 mg, 2.31 mmol) in MeOH (3 mL) and water (3 mL) was heated at 65° C. for 18 hours. After cooling, mixture was diluted with water and concentrated under reduced pressure. Resulting solution was extracted with ethyl acetate (3×5 mL). And water layer was acidified with 2N aqueous HCl, precipitate formed was collected by filtration and washed with water to give 7-(4-methyl-piperazin-1-yl)-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carboxylic acid. (Yield 166 mg, 75%).
A solution of 4-benzylamino-6-chloro-nicotinic acid methyl ester (5.5 g, 19.8 mmol) (from Example 9 supra) in conc. H2SO4 (24 mL) was left standing for 15 minutes at 0° C. Then the dark solution was poured into ice-water (200 mL) while stirring. The solution was neutralized to pH4 using K2CO3. The precipitate was filtered off and the pH of the filtrate was adjusted to 10 with K2CO3, and the solution was extracted with ethyl acetate (300 mL). The extracts were combined and washed with water (100 mL), brine (50 mL) and dried over anhydrous sodium sulfate. The drying agent was removed by filtration and the solvent was evaporated to give 4-amino-6-chloro-nicotinic acid methyl ester which was used directly in the next step without further purification. (Yield 3.8 g, 103%, crude).
1H NMR (300 MHz, CDCl3): δ 8.69 (s, 1H), 6.57 (s, 1H), 3.90 (s, 3H), 1.62 (brs, 2H). LC-MS: [M+H]+ 186.9.
To a stirred suspension of LiAlH4 (1.0 g, 26.3 mmol) in dry THF (500 mL) at 0° C. under nitrogen was added dropwise a solution of 4-amino-6-chloro-nicotinic acid methyl ester (2.6 g, 13.9 mmol) (from Example 22 supra) in THF (100 mL). After the addition, the reaction mixture was stirred at this temperature for 1 hour. The reaction was quenched slowly with water (1.5 mL) and filtered. The organic solution was evaporated to give (4-amino-6-chloro-pyridin-3-yl)-methanol as a white solid which was used directly in the next step. (Yield 2.2 g, 100%).
LC-MS: [M+H]+ 159.0.
To a solution of (4-amino-6-chloro-pyridin-3-yl)-methanol (0.335 g, 2.1 mmol) (from Example 23 supra) in dichloromethane (100 mL) at room temperature was added MnO2 (1.9 g, 21.8 mmol). The reaction mixture was stirred at this temperature for 16 hours then filtered. Solvent was evaporated to give 4-amino-6-chloro-pyridine-3-carbaldehyde as a white solid which was used directly in the next step. (Yield 0.275 g, 83.9%).
1H NMR (300 MHz, CDCl3): δ 9.90 (s, 1H), 8.38 (s, 1H), 6.58 (s, 1H), 1.57 (s, 2H). LC-MS: [M+H]+ 157.1.
To a solution of 4-amino-6-chloro-pyridine-3-carbaldehyde (1.6 g, 10.2 mmol) (from Example 24 supra) in DMSO (10 mL) at room temperature, was added dimethyl malonate (6.4 g, 48.4 mmol) and DL-proline (1.5 g, 13 mmol) successively. The reaction mixture was stirred at room temperature for 16 hours and then at 65° C. for 2 hours. The mixture was poured into water (80 mL), and extracted with dichloromethane (4×100 mL). The organic phase was washed with water (3.×100 mL), brine (50 mL) and dried to give a crude product. It was purified by chromatography (silica gel, 200-300 mesh, eluting with a mixture of petroleum ether and ethyl acetate (3:2, v/v)) to give 7-chloro-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carboxylic acid methyl ester. (Yield 2.0 g, 82.3%).
1H NMR (300 MHz, DMSO): δ 12.41 (s, 1H), 8.86 (s, 1H), 8.64 (s, 1H), 7.22 (s, 1H), 3.83 (s, 3H). LC-MS: [M+H]+ 239.0.
To a stirred solution of 7-chloro-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carboxylic acid methyl ester (0.515 g, 2.15 mmol) (from Example 25 supra) in DMSO (8 mL) was added triethylamine (0.633 g, 6.26 mmol) and C-(2,2-dimethyl-[1,3]dioxolan-4-yl)-methylamine (0.552 g, 4.2 mmol) at room temperature under nitrogen. Then the reaction mixture was stirred at 140° C. for two hours under nitrogen. The mixture was cooled down and poured into water (35 mL), then extracted with dichloromethane (3×100 mL). The organic phase was washed with water (3×80 mL), brine (80 mL) and dried to give 7-[(2,2-dimethyl-[1,3]dioxolan-4-ylmethyl)-amino]-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carboxylic acid methyl ester as a solid which was used directly in the next step without further purification. (Yield 0.61 g, 85.2%).
LC-MS: [M+H]+ 334.0.
To a stirred solution of 7-[(2,2-dimethyl-[1,3]dioxolan-4-ylmethyl)-amino]-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carboxylic acid methyl ester (180 mg, 0.54 mmol) (from Example 26 supra) in methanol (40 mL) was added a solution of sodium hydroxide (50 mg, 12.5 mmol) in water (6 mL) at room temperature. After the addition, the solution was stirred at this temperature for 16 hours. The solution was evaporated at reduced pressure and the residue was diluted with water (20 mL), then extracted with dichloromethane (3×20 mL) to give 7-[(2,2-dimethyl-[1,3]dioxolan-4-ylmethyl)-amino]-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carboxylic acid as a solid which was used directly in the next step without further purification. (Yield 150 mg, 87.2%). LC-MS: [M+H]+ 320.0.
3-[(7-Methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-4-methyl-benzoic acid methyl ester was prepared from 7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carboxylic acid (from Example 15 supra) (2.4 g, 10.9 mmol) and 3-amino-4-methyl-benzoic acid methyl ester by following the method in Example 30. (Yield 3.2 g, 80%).
1H NMR (300 MHz, d6-DMSO) δ 12.12 (s, 1H), 8.97 (s, 2H), 8.89 (s, 1H), 7.64 (dd, 1H, J=7.8, 1.7 Hz), 7.41 (d, 1H, J=8.0 Hz), 6.61 (s, 1H), 3.95 (s, 3H), 3.86 (s, 3H), 2.42 (s, 3H). LC-MS: [M+H]+ 368.
3-[(7-Methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-4-methyl-benzoic acid was prepared from 3-[(7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-4-methyl-benzoic acid methyl ester (from Example 28 supra) (2.5 g, 6.8 mmol) by following the method in Example 31. (Yield 2.0 g, 83%).
1H NMR (300 MHz, d6-DMSO) δ 11.80 (s, 1H), 8.99 (s, 1H), 8.91 (d, 2H, J=5.1 Hz), 7.62 (d, 1H, J=7.6 Hz), 7.37 (d, 1H, J=7.9 Hz), 6.63 (s, 1H), 3.95 (s, 3H), 2.40 (s, 3H). LC-MS: [M+H]+ 354.
Triethylamine (1.16 g, 11.5 mmol) was added to a mixture of 7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carboxylic acid (from Example 15 supra) (2.3 g, 10.45 mmol) and HATU (4.37 g, 11.5 mmol) in dry DMF (30 mL). The resulting mixture was stirred at room temperature until clear solution was obtained. 3-Amino-4-chloro-benzoic acid methyl ester (2.32 g, 12.55 mmol) was added and the resulting mixture was stirred for another 20 hours. The reaction mixture was diluted with water (300 mL), aqueous saturated NaHCO3 (60 mL) and ethyl acetate (150 mL). After thorough mixing, off white precipitate was collected by filtration, washed water, ethyl acetate and MeOH, and dried under reduced pressure to give 4-chloro-3-[(7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-benzoic acid methyl ester as an off-white solid. (Yield 2.9 g, 71.8%).
1H NMR (300 MHz, d6-DMSO) δ 12.42 (s, 1H), 9.18 (s, 1H), 9.01 (s, 1H), 8.91 (s, 1H), 7.70 (d, 2H, J=1.1 Hz), 6.61 (s, 1H), 3.95 (s, 3H), 3.89 (s, 3H). LC-MS: [M+H]+ 388.
A mixture of 4-chloro-3-[(7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-benzoic acid methyl ester (from Example 30 supra) (2.5 g, 6.46 mmol) in MeOH (30 mL) and 4 N aqueous of NaOH (45 mL) was stirred at room temperature overnight. 1H NMR showed that the methyl ester was consumed completely. Reaction mixture was then diluted with water (300 mL), acidified with 1 N aqueous HCl to pH 3-4. Pale yellow precipitate formed was collected by filtration, washed with water and dried under reduced pressure to give 4-chloro-3-[(7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-benzoic acid as a pale yellow solid. (2 g, 83%).
1H NMR (300 MHz, d6-DMSO) δ 12.55 (s, 1H), 12.33 (s, 1H), 9.15 (s, 1H), 9.01 (s, 1H), 8.92 (s, 1H), 7.67 (s, 2H), 6.63 (s, 1H), 3.95 (s, 3H). LC-MS: [M+H]+ 374.
Triethylamine (42.4 mg, 0.42 mmol) was added to a mixture of 4-chloro-3-[(7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-benzoic acid (from Example 31 supra) (80 mg, 0.21 mmol) and HATU (97.6 mg, 0.26 mmol) in dry DMF (7 mL). The resulting solution was stirred at room temperature for 30 minutes with formation of white solid. Benzylamine (34.2 mg, 0.32 mmol) was added and the resulting solution was stirred for another 20 hours with formation of yellow solid. The reaction mixture was diluted with water (70 mL), aqueous saturated NaHCO3 (20 mL) and ethyl acetate (30 mL). After thoroughly mixing, the precipitate was collected by filtration, washed with water, ethyl acetate and purified by refluxing with MeOH (25 mL) for 30 min. The precipitate was collected by filtration, dried under reduced pressure to give 7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carboxylic acid (5-benzylcarbamoyl-2-chloro-phenyl)-amide as a red-white solid. (Yield 50 mg, 50.5%).
1H NMR (300 MHz, d6-DMSO) δ 12.58 (s, 1H), 12.32 (s, 1H), 9.17 (t, 1H, J=5.9 Hz), 9.01 (d, 3H, J=27.2 Hz), 7.68 (s, 2H), 7.38-7.25 (m, 4H), 6.66 (s, 1H), 4.51 (d, 2H, J=5.9 Hz), 3.98 (s, 3H). LC-MS: [M+H]+ 462.8.
7-Methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carboxylic acid (5-benzylcarbamoyl-2-methyl-phenyl)-amide was prepared from 3-[(7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-4-methyl-benzoic acid (from Example 29 supra) (80 mg, 0.22 mmol) and benzylamine by following the method in Example 32. (Yield 60 mg, 60%).
1H NMR (300 MHz, d6-DMSO) δ 12.58 (s, 1H), 11.77 (s, 1H), 9.15-8.92 (m, 3H), 8.77 (s, 1H), 7.61 (dd, 1H, J=7.8 Hz, 1.3 Hz), 7.47-7.18 (m, 6H), 6.66 (s, 1H), 4.50 (d, 2H, J=5.9 Hz), 3.98 (s, 3H), 2.42 (s, 3H). LC-MS: [M+1]+ 442.9.
7-Methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carboxylic acid [5-(2-chloro-benzylcarbamoyl)-2-methyl-phenyl]-amide was prepared from 3-[(7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-4-methyl-benzoic acid (from Example 29 supra) (80 mg, 0.22 mmol) and 2-chloro-benzylamine by following the method in Example 32. (Yield 75 mg, 69%).
1H NMR (300 MHz, d6-DMSO) δ 12.57 (s, 1H), 11.79 (s, 1H), 9.14-8.92 (m, 3H), 8.80 (s, 1H), 7.72-7.59 (m, 1H), 7.57-7.26 (m, 5H), 6.66 (s, 1H), 4.57 (d, 2H, J=5.7 Hz), 3.98 (s, 3H), 2.43 (s, 3H). LC-MS: [M+H]+ 476.9.
7-Methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carboxylic acid [5-(3-chloro-benzylcarbamoyl)-2-methyl-phenyl]-amide was prepared from 3-[(7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-4-methyl-benzoic acid (from Example 29 supra) (80 mg, 0.22 mmol) and 3-chloro-benzylamine by following the method in Example 32. (Yield 55 mg, 51%).
1H NMR (300 MHz, d6-DMSO) δ 12.57 (s, 1H), 11.78 (s, 1H), 9.02 (t, 3H, J=15.3 Hz), 8.78 (s, 1H), 7.61 (d, 1H, J=7.8 Hz), 7.53-7.22 (m, 5H), 6.66 (s, 1H), 4.50 (d, 2H, J=5.8 Hz), 3.98 (s, 3H), 2.42 (s, 3H). LC-MS: [M+H]+ 476.9.
7-Methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carboxylic acid [5-(4-chloro-benzylcarbamoyl)-2-methyl-phenyl]-amide was prepared from 3-[(7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-4-methyl-benzoic acid (from Example 29 supra) (80 mg, 0.22 mmol) and 4-chloro-benzylamine by following the method in Example 32. (Yield 65 mg, 60%).
1H NMR (300 MHz, d6-DMSO) δ 12.58 (s, 1H), 11.78 (s, 1H), 9.02 (t, 3H, J=15.0 Hz), 8.76 (s, 1H), 7.66-7.55 (m, 1H), 7.39 (q, 5H, J=8.6 Hz), 6.66 (s, 1H), 4.48 (d, 2H, J=5.8 Hz), 3.98 (s, 3H), 2.42 (s, 3H). LC-MS: [M+H]+ 476.9.
7-Methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carboxylic acid {2-methyl-5-[(thiophen-2-ylmethyl)-carbamoyl]-phenyl}-amide was prepared from 3-[(7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-4-methyl-benzoic acid (from Example 29 supra) (80 mg, 0.22 mmol) and thiophen-2-yl-methylamine by following the method in Example 32. (Yield 25 mg, 24%).
1H NMR (300 MHz, d6-DMSO) δ 12.58 (s, 1H), 11.77 (s, 1H), 9.21-8.90 (m, 3H), 8.76 (d, 1H, J=1.4 Hz), 7.58 (dd, 1H, J=7.9 Hz, 1.6 Hz), 7.47-7.29 (m, 2H), 7.10-6.89 (m, 2H), 6.66 (s, 1H), 4.65 (d, 2H, J=5.8 Hz), 3.98 (s, 3H), 2.41 (s, 3H). LC-MS: [M+H]+ 448.8.
7-Methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carboxylic acid {2-methyl-5-[(thiophen-3-ylmethyl)-carbamoyl]-phenyl}-amide was prepared from 3-[(7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-4-methyl-benzoic acid (from Example 29 supra) (80 mg, 0.22 mmol) and thiophen-3-yl-methylamine by following the method in Example 32. (Yield 70 mg, 69%).
1H NMR (300 MHz, d6-DMSO) δ 12.56 (s, 1H), 11.76 (s, 1H), 8.96 (t, 3H, J=15.0 Hz), 8.74 (s, 1H), 7.65-7.43 (m, 2H), 7.41-7.26 (m, 2H), 7.10 (d, 1H, J=4.9 Hz), 6.64 (s, 1H), 4.46 (d, 2H, J=5.8 Hz), 3.96 (s, 3H), 2.39 (s, 3H). LC-MS: [M+H]+ 448.7.
7-Methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carboxylic acid [2-chloro-5-(2-chloro-benzylcarbamoyl)-phenyl]-amide was prepared from 4-chloro-3-[(7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-benzoic acid (from Example 31 supra) (80 mg, 0.21 mmol) and 2-chloro-benzylamine by following the method in Example 32. (Yield 55 mg, 52%). 1H NMR (300 MHz, d6-DMSO) δ 12.46 (s, 1H), 12.32 (s, 1H), 9.16 (t, 1H, J=5.7 Hz), 9.06 (d, 2H, J=4.3 Hz), 8.96 (s, 1H), 7.70 (s, 2H), 7.53-7.44 (m, 1H), 7.43-7.27 (m, 3H), 6.65 (s, 1H), 4.56 (d, 2H, J=5.6 Hz), 3.97 (s, 3H). LC-MS: [M+H]+ 496.8.
7-Methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carboxylic acid [2-chloro-5-(3-chloro-benzylcarbamoyl)-phenyl]-amide was prepared from 4-chloro-3-[(7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-benzoic acid (from Example 31 supra) (80 mg, 0.21 mmol) and 3-chloro-benzylamine by following the method in Example 32. (Yield 50 mg, 47%). 1H NMR (300 MHz, d6-DMSO) δ 12.56 (s, 1H), 12.31 (s, 1H), 9.19 (t, 1H, J=5.8 Hz), 9.05 (s, 2H), 8.96 (s, 1H), 7.68 (s, 2H), 7.47-7.26 (m, 4H), 6.65 (s, 1H), 4.49 (d, 2H, J=5.8 Hz), 3.97 (s, 3H). LC-MS: [M+H]+ 496.8.
7-Methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carboxylic acid [2-chloro-5-(4-chloro-benzylcarbamoyl)-phenyl]-amide was prepared from 4-chloro-3-[(7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-benzoic acid (from Example 31 supra) (80 mg, 0.21 mmol) and 4-chloro-benzylamine by following the method in Example 32. (Yield 70 mg, 66%). 1H NMR (300 MHz, d6-DMSO) δ 12.59 (s, 1H), 12.32 (s, 1H), 9.20 (t, 1H, J=5.8 Hz), 9.06 (s, 2H), 8.97 (s, 1H), 7.73-7.63 (m, 2H), 7.40 (q, 4H, J=8.5 Hz), 6.66 (s, 1H), 4.49 (d, 2H, J=5.8 Hz), 3.99 (s, 3H). LC-MS: [M+H]+ 496.8.
7-Methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carboxylic acid {2-chloro-5-[(thiophen-2-ylmethyl)-carbamoyl]-phenyl}-amide was prepared from 4-chloro-3-[(7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-benzoic acid (from Example 31 supra) (80 mg, 0.21 mmol) and thiophen-2-yl-methylamine by following the method in Example 32. (Yield 45 mg, 45%). 1H NMR (300 MHz, d6-DMSO) δ 12.58 (s, 1H), 12.32 (s, 1H), 9.26 (t, 1H, J=5.8 Hz), 9.03 (dd, 3H, J=14.4 Hz, 12.7 Hz), 7.65 (dt, 2H, J=8.4 Hz), 7.50-7.33 (m, 1H), 7.14-6.93 (m, 2H), 6.66 (s, 1H), 4.66 (d, 2H, J=5.7 Hz), 3.98 (s, 3H).
LC-MS: [M+H]+ 468.8.
7-Methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carboxylic acid {2-chloro-5-[(thiophen-3-ylmethyl)-carbamoyl]-phenyl}-amide was prepared from 4-chloro-3-[(7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-benzoic acid (from Example 31 supra) (80 mg, 0.21 mmol) and thiophen-3-yl-methylamine by following the method in Example 32. (Yield 80 mg, 80%). 1H NMR (300 MHz, d6-DMSO) δ 12.56 (s, 1H), 12.31 (s, 1H), 9.22-8.89 (m, 4H), 7.79-7.59 (m, 2H), 7.57-7.45 (m, 1H), 7.36 (s, 1H), 7.12 (d, 1H, J=4.9 Hz), 6.66 (s, 1H), 4.50 (d, 2H, J=5.7 Hz), 3.98 (s, 3H). LC-MS: [M+H]+ 468.8.
(2-{4-Chloro-3-[(7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-benzoylamino}-2-phenyl-ethyl)-carbamic acid tert-butyl ester was prepared from 4-chloro-3-[(7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-benzoic acid (from Example 31 supra) (100 mg, 0.27 mmol) and (2-amino-2-phenyl-ethyl)-carbamic acid tert-butyl ester (from Example 1 supra) by following the method in Example 32. (Yield 100 mg, 63%).
LC-MS: [M-100+1]+ 492.
A mixture of (2-{4-chloro-3-[(7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-benzoylamino}-2-phenyl-ethyl)-carbamic acid tert-butyl ester (from Example 44 supra) (100 mg, 0.17 mmol) in 5 N HCl (15 mL) in dioxane was stirred at room temperature for 20 hours. Then it was concentrated under reduced pressure and the residue was diluted with ethyl acetate (20 mL). The precipitate was collected by filtration. This crude product was purified by refluxing in MeOH (25 mL) for 30 minutes and the precipitate was collected by filtration, dried under reduced pressure to give 7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carboxylic acid [5-(2-amino-1-phenyl-ethylcarbamoyl)-2-chloro-phenyl]-amide hydrochloride as a pale yellow solid. (Yield 55 mg, 57.7%).
1H NMR (300 MHz, d6-DMSO) δ 12.32 (s, 1H), 9.16 (d, 1H, J=8.3 Hz), 9.09-8.93 (m, 3H), 8.14 (s, 2H), 7.79 (dd, 2H, J=32.4 Hz, 8.4 Hz), 7.58-7.28 (m, 5H), 6.68 (s, 1H), 5.39 (t, 1H, J=7.4 Hz), 3.98 (s, 3H), 3.44-3.36 (m, 1H), 3.29-3.17 (m, 1H). LC-MS: [M+H]+ 491.7.
To a stirred solution of 7-[(2,2-dimethyl-[1,3]dioxolan-4-ylmethyl)-amino]-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carboxylic acid (110 mg, 0.344 mmol) (from Example 27 supra) and methyl 3-aminobenzoate (62 mg, 0.41 mmol) in THF (20 mL) was added HATU (157 mg, 0.413 mmol) and DIEA (133 mg, 1.03 mmol) at room temperature under nitrogen. The reaction mixture was stirred at this temperature for sixteen hours. The solvent was removed under reduced pressure and the residue was treated with methanol (20 mL), then stirred at room temperature for 20 minutes, filtered and washed with methanol (10 mL), ethyl acetate (10 mL), dichloromethane (20 mL) and dried to give 3-({7-[(2,2-dimethyl-[1,3]dioxolan-4-ylmethyl)-amino]-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl}-amino)-benzoic acid methyl ester as a white solid which was used directly in the next step without further purification. (Yield 110 mg, 45%). LC-MS: [M+H]+ 453.1.
To a stirred solution of 3-({7-[(2,2-dimethyl-[1,3]dioxolan-4-ylmethyl)-amino]-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl}-amino)-benzoic acid methyl ester (10 mg, 0.022 mmol) (from Example 46 supra) in methanol (10 mL) was added conc. HCl (four drops) at room temperature. The reaction mixture was stirred at this temperature for 16 hours. The solvent was removed under reduced pressure and the residue was treated with dichloromethane (5 mL), methanol (5 mL), and then filtered to give crude product as a solid. It was purified by prep-HPLC to give 3-{[7-(2,3-dihydroxy-propylamino)-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl]-amino}-benzoic acid methyl ester; hydrochloride as a yellow solid. (Yield 8 mg, 81.0%).
1H NMR (300 MHz, DMSO+D2O): δ 8.67 (s, 1H), 8.56 (s, 1H), 8.28 (s, 1H), 7.74 (d, 1H, J=9.3 Hz), 7.65 (d, 1H, J=7.5 Hz), 7.46 (t, 1H, J=7.9 Hz), 6.24 (s, 1H), 3.86 (s, 3H), 3.67-3.63 (m, 1H), 3.41-3.35 (d, 3H), 3.22-3.15 (m, 1H). LC-MS: [M+H]+ 413.0.
3-({7-[(2,2-Dimethyl-[1,3]dioxolan-4-ylmethyl)-amino]-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl}-amino)-4-methyl-benzoic acid methyl ester was prepared from 7-[(2,2-dimethyl-[1,3]dioxolan-4-ylmethyl)-amino]-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carboxylic acid (from Example 27 supra) (130 mg, 0.41 mmol) and 3-amino-4-methyl-benzoic acid methyl ester by following the method in Example 46. (Yield 150 mg, 80.4%).
LC-MS: [M+H]+ 467.1.
3-{[7-(2,3-Dihydroxy-propylamino)-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl]-amino}-4-methyl-benzoic acid methyl ester; hydrochloride was prepared from 3-({7-[(2,2-dimethyl-[1,3]dioxolan-4-ylmethyl)-amino]-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl}-amino)-4-methyl-benzoic acid methyl ester (from Example 48 supra) (0.15 g, 0.32 mmol) by following the method in Example 47. (Yield 0.055 g, 37.1%).
1H NMR (300 MHz, DMSO+D2O): δ 8.89-8.88 (m, 1H), 8.77 (s, 1H), 8.67 (s, 1H), 7.60 (dd, 1H, J1=9.9 Hz, J2=1.5 Hz), 7.39 (d, 1H, J=8.1 Hz), 6.36 (s, 1H), 3.82 (s, 3H), 3.70-3.67 (m, 1H), 3.41-3.68 (m, 3H), 3.27-3.20 (m, 1H). LC-MS: [M+H]+ 427.1.
[2-{4-Chloro-3-[(7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-benzoylamino}-2-(3-chloro-phenyl)-ethyl]-carbamic acid tert-butyl ester was prepared from 4-chloro-3-[(7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-benzoic acid (from Example 31 supra) (100 mg, 0.27 mmol) and [2-amino-2-(3-chloro-phenyl)-ethyl]-carbamic acid tert-butyl ester (from Example 3 supra) by following the method in Example 32. (Yield 85 mg, 51%). LC-MS: [M-100+H]+ 526.
7-Methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carboxylic acid {5-[2-amino-1-(3-chloro-phenyl)-ethylcarbamoyl]-2-chloro-phenyl}-amide; hydrochloride was prepared from [2-{4-chloro-3-[(7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-benzoylamino}-2-(3-chloro-phenyl)-ethyl]-carbamic acid tert-butyl ester (from Example 50 supra) (85 mg, 0.16 mmol) by following the method in Example 45. (Yield 33 mg, 40.6%).
1H NMR (300 MHz, d6-DMSO) δ 12.33 (s, 1H), 9.20 (d, 1H, J=8.2 Hz), 9.03 (dd, 3H, J=12.9 Hz, 10.9 Hz), 8.15 (s, 2H), 7.92-7.70 (m, 2H), 7.59-7.33 (m, 4H), 6.68 (s, 1H), 5.46-5.27 (m, 1H), 3.98 (s, 3H), 3.26 (dd, 2H, J=12.8 Hz, 4.3 Hz). LC-MS: [M+H]+ 525.7.
(3-{4-Chloro-3-[(7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-benzoylamino}-3-thiophen-3-yl-propyl)-carbamic acid tert-butyl ester was prepared from 4-chloro-3-[(7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-benzoic acid (from Example 31 supra) (100 mg, 0.27 mmol) and (3-amino-3-thiophen-3-yl-propyl)-carbamic acid tert-butyl ester (from Example 4 supra) by following the method in Example 32. (Yield 110 mg, 67%). LC-MS: [M-100+1]+ 512.
7-Methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carboxylic acid [5-(3-amino-1-thiophen-3-yl-propylcarbamoyl)-2-chloro-phenyl]-amide was prepared from (3-{4-chloro-3-[(7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-benzoylamino}-3-thiophen-3-yl-propyl)-carbamic acid tert-butyl ester (from Example 52 supra) (110 mg, 0.18 mmol) by following the method in Example 45. The crude product obtained was purified by prep-HPLC (Instrument: Gilson 281, Column. Gemini 5u C18 250×21.5 mm, Mobile Phase: CH3CN/H2O (0.1% TFA), 25/75 to 90/10, flow rate 20 mL/min). The resulting solution of product was evaporated, suspended in saturated aqueous NaHCO3 (10 mL) and stirred for 30 minutes. The solid was collected by filtration and dried to give 7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carboxylic acid [5-(3-amino-1-thiophen-3-yl-propylcarbamoyl)-2-chloro-phenyl]-amide. (Yield 13 mg, 14%).
1H NMR (300 MHz, d6-DMSO) δ 12.76 (s, 1H), 12.42 (s, 1H), 9.28-8.98 (m, 4H), 8.13 (s, 3H), 7.89-7.50 (m, 4H), 7.32 (d, 1H, J=4.9 Hz), 6.79 (s, 1H), 5.41 (q, 1H, J=7.5 Hz), 4.07 (s, 3H), 3.06-2.72 (m, 2H), 2.30 (m, 2H). LC-MS: [M+H]+ 512.1.
(2-{3-[(7-Methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-4-methyl-benzoylamino}-2-phenyl-ethyl)-carbamic acid tert-butyl ester was prepared from 3-[(7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-4-methyl-benzoic acid (from Example 29 supra) (100 mg, 0.28 mmol) and (2-amino-2-phenyl-ethyl)-carbamic acid tert-butyl ester (from Example 1 supra) by following the method in Example 32. (Yield 70 mg, 43%).
LC-MS: [M+H]+ 572.
7-Methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carboxylic acid [5-(2-amino-1-phenyl-ethylcarbamoyl)-2-methyl-phenyl]-amide was prepared from (2-{3-[(7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-4-methyl-benzoylamino}-2-phenyl-ethyl)-carbamic acid tert-butyl ester (from Example 54 supra) (70 mg, 0.12 mmol) by following the method in Example 45. (Yield 16 mg, 24%).
1H NMR (300 MHz, d6-DMSO) δ 11.74 (s, 1H), 9.06-8.83 (m, 3H), 8.71 (s, 1H), 8.11 (s, 2H), 7.72 (d, 1H, J=7.9 Hz), 7.54-7.19 (m, 6H), 6.65 (s, 1H), 5.44-5.24 (m, 1H), 3.94 (s, 3H), 3.37 (s, 1H), 3.17 (dd, 1H, J=12.8 Hz, 3.8 Hz), 2.38 (s, 3H). LC-MS: [M+H]+ 471.8.
(2-(3-Chloro-phenyl)-2-{3-[(7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-4-methyl-benzoylamino}-ethyl)-carbamic acid tert-butyl ester was prepared from 3-[(7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-4-methyl-benzoic acid (from Example 29 supra) (100 mg, 0.28 mmol) and [2-amino-2-(3-chloro-phenyl)-ethyl]-carbamic acid tert-butyl ester (from Example 3 supra) by following the method in Example 32. (Yield 160 mg, 93.6%).
LC-MS: [M-100+1]+ 506.
7-Methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carboxylic acid {5-[2-amino-1-(3-chloro-phenyl)-ethylcarbamoyl]-2-methyl-phenyl}-amide was prepared from (2-(3-chloro-phenyl)-2-{3-[(7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-4-methyl-benzoylamino}-ethyl)-carbamic acid tert-butyl ester (from Example 56 supra) (160 mg, 0.26 mmol) by following the method in Example 45. (Yield 100 mg, 65.5%).
1H NMR (300 MHz, d6-DMSO) δ 12.67 (s, 1H), 11.77 (s, 1H), 9.14 (d, 1H, J=8.0 Hz), 8.97 (d, 2H, J=20.1 Hz), 8.73 (s, 1H), 8.30 (s, 3H), 7.81 (d, 1H, J=7.9 Hz), 7.42-7.40 (m, 5H), 6.66 (d, 4H, J=21.2 Hz), 5.46-5.30 (m, 1H), 3.96 (s, 3H), 3.48-3.11 (m, 2H), 2.40 (s, 3H). LC-MS: [M+H]+ 505.7.
(2-{3-[(7-Methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-4-methyl-benzoylamino}-2-thiophen-3-yl-ethyl)-carbamic acid tert-butyl ester was prepared from 3-[(7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-4-methyl-benzoic acid (from Example 29 supra) (100 mg, 0.28 mmol) and (2-amino-2-thiophen-3-yl-ethyl)-carbamic acid tert-butyl ester (from Example 5 supra) by following the method in Example 32. (Yield 100 mg, 61%). LC-MS: [M-100+1]+ 478.
7-Methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carboxylic acid [5-(2-amino-1-thiophen-3-yl-ethylcarbamoyl)-2-methyl-phenyl]-amide; hydrochloride was prepared from (2-{3-[(7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-4-methyl-benzoylamino}-2-thiophen-3-yl-ethyl)-carbamic acid tert-butyl ester (from Example 58 supra) (100 mg, 0.17 mmol) by following the method in Example 45. (Yield 47 mg, 49.4%).
1H NMR (300 MHz, d6-DMSO) δ 12.66 (s, 1H), 11.77 (s, 1H), 9.08-8.88 (m, 3H), 8.74 (s, 1H), 8.20 (s, 3H), 7.74 (dd, 1H, J=7.9 Hz, 1.2 Hz), 7.63-7.33 (m, 3H), 7.20 (d, 1H, J=4.2 Hz), 6.70 (s, 1H), 5.49 (m, 4.5, 1H), 3.96 (s, 3H), 3.31 (m, 2H), 2.40 (s, 3H). LC-MS: [M+H]+ 477.8.
(3-{3-[(7-Methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-4-methyl-benzoylamino}-3-phenyl-propyl)-carbamic acid tert-butyl ester was prepared from 3-[(7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-4-methyl-benzoic acid (from Example 29 supra) (100 mg, 0.28 mmol) and (3-amino-3-phenyl-propyl)-carbamic acid tert-butyl ester (from Example 2 supra) by following the method in Example 32. (Yield 120 mg, 72.7%). LC-MS: [M+H]+ 586.
7-Methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carboxylic acid [5-(3-amino-1-phenyl-propylcarbamoyl)-2-methyl-phenyl]-amide; hydrochloride was prepared from (3-{3-[(7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-4-methyl-benzoylamino}-3-phenyl-propyl)-carbamic acid tert-butyl ester (from Example 60 supra) (120 mg, 0.20 mmol) by following the method in Example 45. (Yield 80 mg, 70%).
1H NMR (300 MHz, d6-DMSO) δ 12.63 (s, 1H), 11.74 (s, 1H), 8.94 (t, 3H, J=14.6 Hz), 8.69 (s, 1H), 8.03 (s, 3H), 7.61 (d, 1H, J=7.9 Hz), 7.48-7.14 (m, 6H), 6.68 (s, 1H), 5.14 (q, 1H, J=8.1 Hz), 3.94 (s, 3H), 2.77 (m, 3H), 2.37 (s, 3H), 2.30-2.01 (m, 2H). LC-MS: [M+H]+ 485.9.
(3-{3-[(7-Methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-4-methyl-benzoylamino}-3-thiophen-3-yl-propyl)-carbamic acid tert-butyl ester was prepared from 3-[(7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-4-methyl-benzoic acid (from Example 29 supra) (100 mg, 0.28 mmol) and (3-amino-3-thiophen-3-yl-propyl)-carbamic acid tert-butyl ester (from Example 4 supra) by following the method in Example 32. (Yield 100 mg, 60%). LC-MS: [M+H]+ 592.
7-Methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carboxylic acid [5-(3-amino-1-thiophen-3-yl-propylcarbamoyl)-2-methyl-phenyl]-amide was prepared from (3-{3-[(7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-4-methyl-benzoylamino}-3-thiophen-3-yl-propyl)-carbamic acid tert-butyl ester (from Example 62 supra) (100 mg, 0.17 mmol) by following the method in Example 45. The crude product obtained was purified by prep-HPLC (Instrument: Gilson 281, Column. Gemini 5u C18 250×21.5 mm, Mobile Phase: CH3CN/H2O (0.1% TFA), 28/72 to 90/10, flow rate 20 mL/min). The resulting solution of product was evaporated, suspended in saturated aqueous NaHCO3 (10 mL) and stirred for 30 minutes. The solid was collected by filtration and dried to give 7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carboxylic acid [5-(3-amino-1-thiophen-3-yl-propylcarbamoyl)-2-methyl-phenyl]-amide. (Yield 10 mg, 12%).
1H NMR (300 MHz, d6-DMSO) δ 12.04 (d, 1H, J=16.5 Hz), 9.05-8.78 (m, 2H), 8.77-8.62 (m, 1H), 7.63-7.44 (m, 2H), 7.35 (d, 2H, J=7.9 Hz), 7.17 (t, 1H, J=4.5 Hz), 6.61 (s, 1H), 5.27 (m, 1H), 3.95 (s, 3H), 2.98 (m, 1H), 2.62 (m, 1H), 2.39 (s, 3H), 1.94 (m, 2H). LC-MS: [M+H]+ 492.1.
(3-(3-Chloro-phenyl)-3-{3-[(7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-4-methyl-benzoylamino}-propyl)-carbamic acid tert-butyl ester was prepared from 3-[(7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-4-methyl-benzoic acid (from Example 29 supra) (100 mg, 0.28 mmol) and [3-amino-3-(3-chloro-phenyl)-propyl]-carbamic acid tert-butyl ester (from Example 6 supra) by following the method in Example 32. (Yield 100 mg, 57%). LC-MS: [M-100+1]+ 520.
7-Methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carboxylic acid {5-[3-amino-1-(3-chloro-phenyl)-propylcarbamoyl]-2-methyl-phenyl}-amide; hydrochloride was prepared from (3-(3-chloro-phenyl)-3-{3-[(7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-4-methyl-benzoylamino}-propyl)-carbamic acid tert-butyl ester (from Example 64 supra) (100 mg, 0.16 mmol) by following the method in Example 45. (Yield 30 mg, 31.6%).
1H NMR (300 MHz, d6-DMSO) δ 12.64 (s, 1H), 11.78 (s, 1H), 9.11-8.88 (m, 3H), 8.72 (d, 1H, J=1.7 Hz), 8.01 (s, 3H), 7.72-7.50 (m, 2H), 7.46-7.29 (m, 4H), 6.68 (s, 1H), 5.17 (m, 1H), 3.96 (s, 3H), 2.79 (m, 2H), 2.40 (s, 3H), 2.28-2.03 (m, 2H). LC-MS: [M+H]+ 518.1.
[3-{4-Chloro-3-[(7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-benzoylamino}-3-(3-chloro-phenyl)-propyl]-carbamic acid tert-butyl ester was prepared from 4-chloro-3-[(7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-benzoic acid (from Example 31 supra) (100 mg, 0.27 mmol) and [3-amino-3-(3-chloro-phenyl)-propyl]-carbamic acid tert-butyl ester (from Example 6 supra) by following the method in Example 32. (Yield 100 mg, 58%).
LC-MS: [M-100+1]+ 540.
7-Methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carboxylic acid {5-[3-amino-1-(3-chloro-phenyl)-propylcarbamoyl]-2-chloro-phenyl}-amide; hydrochloride was prepared from [3-{4-chloro-3-[(7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-benzoylamino}-3-(3-chloro-phenyl)-propyl]-carbamic acid tert-butyl ester (from Example 66 supra) (100 mg, 0.16 mmol) by following the method in Example 45. (Yield 60 mg, 62%).
1H NMR (300 MHz, d6-DMSO) δ 12.65 (s, 1H), 12.32 (s, 1H), 9.15 (d, 1H, J=8.1 Hz), 9.00 (dd, 3H, J=13.9 Hz, 12.0 Hz), 8.04 (s, 3H), 7.71 (dt, 2H, J=12.7 Hz, 5.1 Hz), 7.54 (s, 1H), 7.48-7.29 (m, 3H), 6.68 (s, 1H), 5.15 (m, 1H), 3.96 (s, 3H), 2.80 (m, 2H), 2.16 (m, 2H). LC-MS: [M+H]+ 540.1.
(3-{4-Chloro-3-[(7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-benzoylamino}-3-phenyl-propyl)-carbamic acid tert-butyl ester was prepared from 4-chloro-3-[(7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-benzoic acid (from Example 31 supra) (100 mg, 0.27 mmol) and (3-amino-3-phenyl-propyl)-carbamic acid tert-butyl ester (from Example 2 supra) by following the method in Example 32. (Yield 120 mg, 74%). LC-MS: [M+H]+ 606.
7-Methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carboxylic acid [5-(3-amino-1-phenyl-propylcarbamoyl)-2-chloro-phenyl]-amide; hydrochloride was prepared from (3-{4-chloro-3-[(7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-benzoylamino}-3-phenyl-propyl)-carbamic acid tert-butyl ester (from Example 68 supra) (120 mg, 0.20 mmol) by following the method in Example 45. (Yield 34 mg, 29.6%).
1H NMR (300 MHz, d6-DMSO) δ 12.61 (s, 1H), 12.30 (s, 1H), 9.15-8.87 (m, 4H), 7.91 (s, 3H), 7.68 (s, 2H), 7.36 (m, 5H), 6.66 (s, 1H), 5.16 (m, 1H), 3.96 (d, 3H, J=0.5 Hz), 2.79 (m, 2H), 2.16 (m, 2H). LC-MS: [M+H]+ 506.1.
(2-{4-Chloro-3-[(7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-benzoylamino}-2-thiophen-3-yl-ethyl)-carbamic acid tert-butyl ester was prepared from 4-chloro-3-[(7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-benzoic acid (from Example 31 supra) (100 mg, 0.27 mmol) and (2-amino-2-thiophen-3-yl-ethyl)-carbamic acid tert-butyl ester (from Example 5 supra) by following the method in Example 32. (Yield 100 mg, 62.5%). LC-MS: [M-100+H]+ 498.
7-Methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carboxylic acid [5-(2-amino-1-thiophen-3-yl-ethylcarbamoyl)-2-chloro-phenyl]-amide; hydrochloride was prepared from (2-{4-chloro-3-[(7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-benzoylamino}-2-thiophen-3-yl-ethyl)-carbamic acid tert-butyl ester (from Example 70 supra) (100 mg, 0.17 mmol) by following the method in Example 45. (Yield 60 mg, 63%).
1H NMR (301 MHz, d6-DMSO+D2O) δ 9.03 (s, 2H), 8.93 (s, 1H), 7.73 (q, 2H, J=8.5 Hz), 7.55 (dd, 2H, J=15.0 Hz, 10.6 Hz), 7.19 (d, 1H, J=4.9 Hz), 6.66 (s, 1H), 5.47 (t, 1H, J=7.2 Hz), 3.95 (s, 3H), 3.31 (d, J=7.4 Hz, 2H). LC-MS: [M+H]+ 498.1.
Et3N (39 mg, 0.382 mmol) was added to a mixture of 7-(4-methyl-piperazin-1-yl)-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carboxylic acid (100 mg, 0.347 mmol) (from Example 21 supra) and HATU (145 mg, 0.382 mmol) in dry DMF (4 mL) at room temperature. The resultant mixture was stirred until clear solution was obtained. 3-Amino-benzoic acid methyl ester (63 mg, 0.416 mmol) was added. The mixture was stirred for another 20 hours with formation of precipitate. Water (10 mL), aqueous saturated sodium bicarbonate solution (10 mL), and ethyl acetate (25 mL) were added. After thorough stirring, white precipitate was collected by filtration, washed with water and ethyl acetate and dried under reduced pressure to give 3-{[7-(4-methyl-piperazin-1-yl)-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl]-amino}-benzoic acid methyl ester. (Yield 88 mg, 60%).
1H NMR (300 MHz, d6-DMSO): δ 12.24 (s, 1H), 11.98 (s, 1H), 8.77 (d, 2H, J=10.5 Hz), 8.34 (s, 1H), 7.88 (d, 1H, J=5.1 Hz), 7.68 (d, 1H, J=6.8 Hz), 7.52-7.47 (m, 1H), 6.39 (s, 1H), 3.86 (s, 3H), 3.59 (s, 4H), 2.20 (s, 4H), 1.97 (s, 3H). LC-MS: [M+H]+ 421.8.
4-Chloro-3-{[7-(4-methyl-piperazin-1-yl)-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl]-amino}-benzoic acid methyl ester was prepared from 7-(4-methyl-piperazin-1-yl)-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carboxylic acid (from Example 21 supra) (50 mg, 0.17 mmol) and 3-amino-4-chloro-benzoic acid methyl ester by following the method in Example 72. (Yield 20 mg, 25%).
1H NMR (300 MHz, d6-DMSO): δ 12.49 (s, 1H), 9.21 (s, 1H), 8.86 (s, 1H), 8.79 (s, 1H), 7.70 (s, 2H), 6.42 (s, 1H), 3.89 (s, 3H), 3.63 (s, 4H), 2.42 (s, 4H), 2.23 (s, 3H). LC-MS: [M+H]+ 455.8.
4-Methyl-3-{[7-(4-methyl-piperazin-1-yl)-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl]-amino}-benzoic acid methyl ester was prepared from 7-(4-methyl-piperazin-1-yl)-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carboxylic acid (from Example 21 supra) (66 mg, 0.23 mmol) and 3-amino-4-methyl-benzoic acid methyl ester by following the method in Example 72. (Yield 69 mg, 69%).
1H NMR (300 MHz, d6-DMSO): δ 12.23 (s, 1H), 11.94 (s, 1H), 8.98 (s, 1H), 8.85 (s, 1H), 8.79 (s, 1H), 7.65-7.62 (m, 1H), 7.40 (d, 1H, J=8.1 Hz), 6.42 (s, 1H), 3.86 (s, 3H), 3.62 (s, 4H), 2.42 (s, 7H), 2.23 (s, 3H). LC-MS: [M+H]+ 435.7.
(6-Chloro-3-formyl-pyridin-2-yl)-carbamic acid tert-butyl ester was prepared according to the literature procedure of Siegel, S. et al. WO2009/007029, Jan. 15, 2009.
Sodium hexamethyl disilazane (63.03 g, 343 mmol) was added portion-wise to a stirred solution of 2-chloro-6-aminopyridine (20.0 g, 156.25 mmol) in THF (100 mL) at 0° C. under argon. After 5 minutes of stirring at the same temperature, di-tertiary-butyl dicarbonate (36.77 mL, 171 mmol) was added drop-wise into the reaction mixture. After 15 minutes of additional stirring at the same temperature, the reaction temperature was brought to room temperature and stirred until starting material was consumed completely (1 hour, monitored by silica gel TLC using ethyl acetate-hexanes, 1:9 as mobile phase). THF was distilled off under reduced pressure, the obtained residue was taken up in ethyl acetate (300 mL), washed with an aqueous solution of 0.5 M HCl (100 mL), water (2×75 mL), dried over anhydrous sodium sulfate, filtered and concentrated on the rotary evaporator to obtain a gummy mass, which was purified over silica gel column chromatography (eluant:EtOAc-hexanes, 1:19) to give (6-chloro-pyridin-2-yl)-carbamic acid tert-butyl ester as a white solid. (Yield 31.0 g, 87.4%).
(6-Chloro-pyridin-2-yl)-carbamic acid tert-butyl ester (5.0 g, 21.92 mmol) and 1,2-bis(di-ethylamino)ethane (6.37 g, 54.82 mmol) were dissolved in THF (100 mL) at room temperature under nitrogen and the reaction mixture was cooled to −78° C. To this cooled solution, was added n-butyl lithium (1.8 M in hexanes, 21.92 mL, 54.82 mmol) drop-wise. After completion of addition of n-BuLi, reaction temperature was slowly warmed to −10° C. and held at −10° C. for 2 hours. It was then re-cooled again to −78° C. and DMF (3.39 mL, 43.84 mmol) was added. After completion of addition of DMF, the reaction mixture was again warmed to room temperature slowly and stirring was continued at that temperature until the completion of reaction (1 h, as monitored by silica gel TLC, using ethyl acetate-hexanes 2:8 as mobile phase). Reaction mixture was quenched with aqueous HCl (1N; 30 mL) at −10° C. until the reaction medium was acidic (pH 2-3) and was extracted with ethyl acetate (100 mL). The organic part was separated, washed with water (2×50 mL), brine (50 mL), dried over anhydrous sodium sulfate, filtered and concentrated on the rotary evaporator to give (6-chloro-3-formyl-pyridin-2-yl)-carbamic acid tert-butyl ester as a solid. The obtained product is pure enough to proceed to the next reaction without further purification. (Yield 6.0 g, 99%).
To a stirred solution of (6-chloro-3-formyl-pyridin-2-yl)-carbamic acid tert-butyl ester (25.2 g, 98.43 mmol) (from Example 75 supra) in methanol (504 mL) was added sodium methoxide (8.505 g, 157.5 mmol) at room temperature under nitrogen and the reaction mixture was stirred overnight at the same temperature. Silica gel TLC analysis confirmed the completion of reaction (mobile phase:ethyl acetate-hexanes 3:7). Methanol was distilled off under reduced pressure and the crude organic mixture was taken up in EtOAc (500 mL). The organic part was washed with water (2×100 mL), dried over sodium sulfate, filtered and concentrated under reduced pressure to give crude product, which was purified over silica gel column chromatography (eluant:EtOAc-Hexanes 1:19) to afford (3-formyl-6-methoxy-pyridin-2-yl)-carbamic acid tert-butyl ester as a yellow solid. (Yield 16.68 g, 67.2%).
LC-MS (ES+) [M+H]+ 253.
A solution of HCl in dioxane (3.5 M solution; 600 mL) was added to (3-formyl-6-methoxy-pyridin-2-yl)-carbamic acid tert-butyl ester (20 g, 79.36 mmol) at room temperature under nitrogen and the solution was stirred overnight at the same temperature. Silica gel TLC analysis confirmed the completion of reaction (mobile phase:ethyl acetate-hexanes 2:8). Reaction mixture was concentrated under reduced pressure and the obtained gummy mass was triturated with diethyl ether to obtain crude 2-amino-6-methoxy-pyridine-3-carbaldehyde as an off-white solid. (Yield 14.13 g). LC-MS (ES+) [M+H]+ 153.
Dimethyl malonate (21.5 mL, 188.28 mmol), piperidine (19.3 mL, 195.24 mmol) and acetic acid (0.40 mL, 6.97 mmol) were added simultaneously to a stirred solution of crude 2-amino-6-methoxy-pyridine-3-carbaldehyde (10.6 g, 69.73 mmol) (from Example 76 supra) in methanol (328 mL) at room temperature and the reaction mixture was heated at 60° C. for 18 hours. Silica gel TLC analysis confirmed the completion of reaction (mobile phase: methanol-dichloromethane 1:9). Reaction temperature was cooled to room temperature and solvents were distilled off under reduced pressure to obtain a crude 7-methoxy-2-oxo-1,2-dihydro-[1,8]naphthyridine-3-carboxylic acid methyl ester, which was directly used for the next step reaction without further purification. (Yield 21 g, crude).
LC-MS (ES+) [M+H]+ 235.
A mixture of crude 7-methoxy-2-oxo-1,2-dihydro-[1,8]naphthyridine-3-carboxylic acid methyl ester (21 g, 89.74 mmol) (from Example 77 supra) and sodium hydroxide (10.76 g, 269.23 mmol) in methanol-water mixture (1:1; 370 mL) was heated at 65° C. for 18 hours. After cooling to room temperature, the reaction mixture was concentrated under reduced pressure. The resulting material was acidified (pH ˜2-3) with the drop-wise addition of an aqueous solution of 2N HCl, during which time a white precipitate was formed and was collected by filtration. The collected material was washed with water and dried in air to give 7-methoxy-2-oxo-1,2-dihydro-[1,8]naphthyridine-3-carboxylic acid as a pale yellow solid. (Yield 9.2 g, 46.6%).
LC-MS (ES+) [M+H]+ 221.
To a stirred solution of (6-chloro-3-formyl-pyridin-2-yl)-carbamic acid tert-butyl ester (6.0 g, 23.43 mmol) (from Example 75 supra) in DMSO (100 mL) were added 1-methylpiperizine (3.05 g, 30.40 mmol) and potassium carbonate (6.47 g, 46.8 mmol) at room temperature and the reaction mixture was stirred at 80° C. for 3 hours. (Silica gel TLC analysis using MeOH-dichloromethane 1:9). Reaction mixture was quenched with water (500 mL) and was extracted with dichloromethane (3×100 mL). Combined organic parts were washed with water (100 mL), brine (100 mL), dried over sodium sulfate and evaporated under reduced pressure to give [3-formyl-6-(4-methyl-piperazin-1-yl)-pyridin-2-yl]-carbamic acid tert-butyl ester as a yellow solid. (Yield 4.0 g, 53.3%).
LC-MS (ES+) [M+H]+ 321.
A solution of HCl in dioxane (3.5 M solution; 400 mL) was added to [3-formyl-6-(4-methyl-piperazin-1-yl)-pyridin-2-yl]-carbamic acid tert-butyl ester (10 g, 31.0 mmol) at room temperature under nitrogen and stirred overnight at the same temperature. Silica gel TLC analysis confirmed the completion of reaction (MeOH-dichloromethane 1:9). Reaction mixture was concentrated under reduced pressure to give crude 2-amino-6-(4-methyl-piperazin-1-yl)-pyridine-3-carbaldehyde. (Yield 9.2 g). LC-MS (ES+) [M+H]+ 221.
Dimethyl malonate (9.93 mL, 86.97 mmol), piperidine (9.31 mL, 94.08 mmol) and acetic acid (0.20 mL, 3.55 mmol) were added simultaneously to a stirred solution of crude 2-amino-6-(4-methyl-piperazin-1-yl)-pyridine-3-carbaldehyde (6.9 g, 31.36 mmol) (from Example 79 supra) in methanol (100 mL) at room temperature and the reaction mixture was heated at 60° C. for 18 hours. Silica gel TLC analysis confirmed the completion of reaction (MeOH-dichloromethane 1:9). Mixture was cooled to room temperature and solvents were distilled off under reduced pressure to obtain crude 7-(4-methyl-piperazin-1-yl)-2-oxo-1,2-dihydro-[1,8]naphthyridine-3-carboxylic acid methyl ester which was directly used in the next step without further purification. (Yield 11.0 g). LC-MS (ES+) [M+H]+ 303.
A mixture of crude 7-(4-methyl-piperazin-1-yl)-2-oxo-1,2-dihydro-[1,8]naphthyridine-3-carboxylic acid methyl ester (22.0 g, 72.8 mmol) (from Example 80 supra) and sodium hydroxide (8.741 g, 218.54 mmol) in methanol-water mixture (1:1; 220 mL) was heated at 65° C. for 18 hours. Reaction went to completion during the time (silica gel TLC analysis, MeOH-dichloromethane 1:9). After cooling to room temperature, the reaction mixture was concentrated under reduced pressure, the obtained material was diluted with water (100 mL) and was extracted with dichloromethane (2×100 mL) [to get rid of unwanted organic impurities]. Organic part was discarded and the aqueous layer was acidified with an aqueous solution of HCl (2N, to pH ˜2-3), during which a white precipitate was formed, which was collected by filtration. The residue was washed with water and dried in air to give 7-(4-methyl-piperazin-1-yl)-2-oxo-1,2-dihydro-[1,8]naphthyridine-3-carboxylic acid; hydrochloride as a yellow solid. (Yield 10.0 g, 47.8%). LC-MS (ES+) [M+H]+ 289.
2-Amino-6-chloro-pyridine-3-carbaldehyde was prepared from (6-chloro-3-formyl-pyridin-2-yl)-carbamic acid tert-butyl ester (4.0 g, 15.62 mmol) (from Example 75 supra) in 4 M solution of HCl in dioxane (108 mL, 437 mmol) by following method in Example 76 as an off-white solid. (Yield 3 g, quantitative). LC-MS [M+H]+ 157.
To a cold (0° C.) solution of 2-amino-6-chloro-pyridine-3-carbaldehyde was prepared from (6-chloro-3-formyl-pyridin-2-yl)-carbamic acid tert-butyl ester (3.0 g, 19.23 mmol) (from Example 82 supra) in dichloromethane (90 mL) were added dry pyridine (4.66 mL, 57.69 mmol) and methyl malonyl chloride (6.66 mL, 61.53 mmol) at room temperature under nitrogen and stirred overnight at the same temperature. Silica gel TLC analysis confirmed the completion of reaction. The reaction mixture was quenched with the addition of an aqueous solution of saturated NaHCO3 (50 mL) and was extracted with dichloromethane (3×50 mL). Collected organic parts were dried over sodium sulfate, filtered and concentrated under reduced pressure to give a crude material, which was purified by column chromatography, using ethyl acetate-hexanes 2:3 as eluent, to give 7-chloro-2-oxo-1,2-dihydro-[1,8]naphthyridine-3-carboxylic acid methyl ester as a pale yellow solid. (Yield 2.9 g, 63.4%).
LC-MS [M+H]+ 239.
To a stirred solution of 7-chloro-2-oxo-1,2-dihydro-[1,8]naphthyridine-3-carboxylic acid methyl ester (3.0 g, 12.6 mmol) (from Example 83 supra) in DMSO (60 mL) was added triethylamine (3.49 mL, 25.2 mmol) and (2,2-dimethyl-[1,3]dioxolan-4-yl)-methylamine (2.15 g, 16.38 mmol) at room temperature and reaction mixture was heated at 140° C. until starting material was consumed completely (2 hour; as monitored by silica gel TLC analysis). Reaction mixture was cooled to room temperature, poured into cold water and was extracted with dichloromethane (3×50 mL). Separated organic parts were combined, dried over sodium sulfate, filtered and concentrated to give a crude gummy mass, which was purified by column chromatography, using methanol-dichloromethane 1:49 as eluent, to afford 7-[(2,2-dimethyl-[1,3]dioxolan-4-ylmethyl)-amino]-2-oxo-1,2-dihydro-[1,8]naphthyridine-3-carboxylic acid methyl ester as a pale yellow solid. (Yield 2.3 g, 54.8%).
LC-MS (ES+) [M+H]+ 334.
7-(2,3-Dihydroxy-propylamino)-2-oxo-1,2-dihydro-[1,8]naphthyridine-3-carboxylic acid was prepared from 7-[(2,2-dimethyl-[1,3]dioxolan-4-ylmethyl)-amino]-2-oxo-1,2-dihydro-[1,8]naphthyridine-3-carboxylic acid methyl ester (1.0 g, 3 mmol) (from Example 84 supra) and sodium hydroxide (0.36 g, 9 mmol) in methanol-water mixture (1:1, 25 mL) by the method in Example 78 as a white solid. (Yield 0.67 g, 79.96%). LC-MS (ES+) [M+H]+ 280.
To a stirred solution of 7-(4-methyl-piperazin-1-yl)-2-oxo-1,2-dihydro-[1,8]naphthyridine-3-carboxylic acid; hydrochloride (0.15 g, 0.46 mmol) (from Example 81 supra) and HATU (0.238 g, 0.62 mmol) in dry DMF (8 mL) was added triethylamine (0.2 mL, 1.3 mmol) at room temperature. After 15 minutes stirring at the same temperature, 3-amino-benzoic acid methyl ester (0.0945 g, 0.62 mmol) was added to this mixture. Stirring was continued for another 20 hours, during which time the reaction went for completion with the formation of precipitate. Water (50 mL), aqueous solution of saturated sodium bicarbonate (10 mL) and ethyl acetate (25 mL) were added. After thorough mixing, white precipitate was collected by filtration, washed with water and ethyl acetate and dried under reduced pressure to give 3-{[7-(4-Methyl-piperazin-1-yl)-2-oxo-1,2-dihydro-[1,8]naphthyridine-3-carbonyl]-amino}-benzoic acid methyl ester. (Yield 0.20 g, 91.2%). LC-MS (ES+) [M+H]+ 422.
4-Methyl-3-{[7-(4-methyl-piperazin-1-yl)-2-oxo-1,2-dihydro-[1,8]naphthyridine-3-carbonyl]-amino}-benzoic acid methyl ester was prepared by the method in Example 86 from 7-(4-methyl-piperazin-1-yl)-2-oxo-1,2-dihydro-[1,8]naphthyridine-3-carboxylic acid; hydrochloride (0.1 g, 0.3 mmol) (from Example 81 supra), 3-amino-4-methyl-benzoic acid methyl ester (0.071 g, 0.43 mmol), HATU (0.163 g, 0.43 mmol) and triethylamine (0.12 mL, 0.89 mmol) in dry DMF (5 mL). (Yield 0.06 g, 40%).
LC-MS (ES+) [M+H]+ 436.
4-Chloro-3-{[7-(4-methyl-piperazin-1-yl)-2-oxo-1,2-dihydro-[1,8]naphthyridine-3-carbonyl]-amino}-benzoic acid methyl ester was prepared by the method in Example 86 from 7-(4-methyl-piperazin-1-yl)-2-oxo-1,2-dihydro-[1,8]naphthyridine-3-carboxylic acid; hydrochloride (0.1 g, 0.3 mmol) (from Example 81 supra), 3-amino-4-chloro-benzoic acid methyl ester (0.079 g, 0.43 mmol), HATU (0.163 g, 0.43 mmol) and triethylamine (0.12 mL, 0.89 mmol) in dry DMF (5 mL). (Yield 0.070 g, 44.3%).
LC-MS (ES+) [M+H]+ 456.
3-[(7-Methoxy-2-oxo-1,2-dihydro-[1,8]naphthyridine-3-carbonyl)-amino]-4-methyl-benzoic acid methyl ester was prepared by the method in Example 86 from 7-methoxy-2-oxo-1,2-dihydro-[1,8]naphthyridine-3-carboxylic acid (0.2 g, 0.91 mmol) (from Example 78 supra), 3-amino-4-methyl-benzoic acid methyl ester (0.18 g, 1.09 mmol), HATU (0.0.415 g, 1.09 mmol) and triethylamine (0.3 mL, 2.27 mmol) in dry DMF (8 mL). (Yield 0.203 g, 60.8%).
LC-MS (ES+) [M+H]+ 368.
4-Chloro-3-[(7-methoxy-2-oxo-1,2-dihydro-[1,8]naphthyridine-3-carbonyl)-amino]-benzoic acid methyl ester was prepared by the method in Example 86 from 7-methoxy-2-oxo-1,2-dihydro-[1,8]naphthyridine-3-carboxylic acid (0.2 g, 0.91 mmol) (from Example 78 supra), 3-amino-4-chloro-benzoic acid methyl ester (0.202 g, 1.09 mmol), HATU (0.0.415 g, 1.09 mmol) and triethylamine (0.3 mL, 2.27 mmol) in dry DMF (8 mL). (Yield 0.127 g, 36.1%).
LC-MS (ES+) [M+H]+ 388.
3-[(7-Methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carbonyl)-amino]-benzoic acid methyl ester was prepared from 7-methoxy-2-oxo-1,2-dihydro-[1,6]naphthyridine-3-carboxylic acid (from Example 15 supra) (44 mg, 0.21 mmol) and 3-amino-benzoic acid methyl ester by following the method in Example 30. (Yield 30 mg, 42%).
1H NMR (300 MHz, d6-DMSO) δ 11.94 (s, 1H), 8.97 (s, 1H), 8.91 (s, 1H), 8.38 (s, 1H), 7.92 (d, 1H, J=9.3 Hz), 7.72 (d, 1H, J=7.8 Hz), 7.55-7.51 (m, 1H), 6.62 (s, 1H), 3.95 (s, 3H), 3.88 (s, 3H). LC-MS: [M+H]+ 354.
Triethylamine (0.71 mL, 5.09 mmol) was added to a mixture of 7-methoxy-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carboxylic acid (from Example 78 supra) (1.0 g, 4.54 mmol) and HATU (1.93 g, 5.09 mmol) in DMF (10 mL). Mixture was stirred at room temperature for 30 minutes. Methyl 3-amino-4-chlorobenzoate (1.01 g, 5.45 mmol) was added. Mixture was stirred at room temperature for 18 hours and then partitioned between ethyl acetate and water. The precipitate was collected by filtration, washed with water and ethyl acetate and dried in vacuum oven to give methyl 4-chloro-3-(7-methoxy-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carboxamido)benzoate. (Yield 1.4 g, 79.5%).
LR-MS: [M+H]+ 388.
A solution of methyl 4-chloro-3-(7-methoxy-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carboxamido)benzoate (1.38 g, 3.56 mmol) in methanol (100 mL) was added 2N NaOH solution (15.0 mL). Mixture was heated at reflux for 18 hours. After cooling, mixture was concentrated under reduced pressure to remove most of the methanol. The residue was diluted with water (20 mL) and acidified with 1N hydrochloric acid solution to pH 2. Brown precipitate formed was collected by filtration and washed with water and dried in vacuum oven to give 4-chloro-3-(7-methoxy-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carboxamido) benzoic acid as brown powder. (Yield 1.21 g, 91.0%).
Triethylamine (0.71 mL, 5.09 mmol) was added to a mixture of 7-methoxy-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carboxylic acid (from Example 78 supra) (1.0 g, 4.54 mmol) and HATU (1.93 g, 5.09 mmol) in DMF (10 mL). The mixture was stirred at room temperature for 30 minutes. Methyl 3-amino-4-methylbenzoate (0.90 g, 5.45 mmol) was added. The mixture was stirred at room temperature for 18 hours and then partitioned between ethyl acetate and water. Precipitate was collected by filtration, washed with water and ethyl acetate and dried in vacuum oven to give methyl 3-(7-methoxy-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carboxamido)-4-methylbenzoate. (Yield 1.36 g, 81.5%).
A solution of methyl 3-(7-methoxy-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carboxamido)-4-methylbenzoate (1.33 g, 3.62 mmol) in methanol (100 mL) was added 2N NaOH solution (15.0 ml). Mixture was heated at 50° C. for 18 hours. After cooling, the mixture was concentrated under reduced pressure to remove most of the methanol. The residue was diluted with water (20 mL) and acidified with 1N hydrochloric acid solution to pH 2. Yellow precipitate formed was collected by filtration and washed with water and dried in vacuum oven to give 3-(7-methoxy-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carboxamido)-4-methylbenzoic acid. (Yield 1.22 g, 95.4%).
HR-MS (ES+) m/z Calculated for C18H16N3O5 ([M+H]+): 354.1085. Found: 354.1085.
Triethylamine (0.021 ml, 0.15 mmol) was added to a mixture of 4-chloro-3-(7-methoxy-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carboxamido)benzoic acid (from Example 92 supra) (0.05 g, 0.13 mmol) and HATU (0.057 g, 0.15 mmol) in DMF (2 mL). Mixture was stirred at room temperature for 30 minutes. Benzylamine (0.017 g, 0.16 mmol) was added. Mixture was stirred at room temperature for 18 hours and then partitioned between ethyl acetate and water. The aqueous phase was extracted with ethyl acetate (3×). The combined organic phase was washed with water and brine, dried (magnesium sulfate) and concentrated. The residue was collected by filtration, washed with ethyl acetate and water and dried in vacuum oven to give N-(5-(benzylcarbamoyl)-2-chlorophenyl)-7-methoxy-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carboxamide. (Yield 0.051 g, 82.4%).
HR-MS (ES+) m/z Calculated for C24H19ClN4NaO4 ([M+Na]+): 485.0987. Found: 485.0988.
Triethylamine (0.021 mL, 0.15 mmol) was added to a mixture of 4-chloro-3-(7-methoxy-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carboxamido)benzoic acid (from Example 92 supra) (0.05 g, 0.13 mmol) and HATU (0.057 g, 0.15 mmol) in DMF (2 mL). Mixture was stirred at room temperature for 30 minutes. 3-Chlorobenzylamine (0.023 g, 0.16 mmol) was added. Mixture was stirred at room temperature for 18 hours and then partitioned between ethyl acetate and water. The aqueous phase was extracted with ethyl acetate (3×). The combined organic phase was washed with water and brine, dried (magnesium sulfate) and concentrated. The residue was collected by filtration, washed with ethyl acetate and water and dried in vacuum oven to give N-(2-chloro-5-(3-chlorobenzylcarbamoyl)phenyl)-7-methoxy-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carboxamide. (Yield 0.052 g, 78.2%).
HR-MS (ES+) m/z Calculated for C24H18Cl2N4NaO4 ([M+Na]+): 519.0597. Found: 519.0597.
Triethylamine (0.042 mL, 0.30 mmol) was added to a mixture of 4-chloro-3-(7-methoxy-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carboxamido)benzoic acid (from Example 92 supra) (0.10 g, 0.27 mmol) and HATU (0.11 g, 0.30 mmol) in DMF (4 mL). Mixture was stirred at room temperature for 30 minutes. (3-Amino-3-phenyl-propyl)-carbamic acid tert-butyl ester (from Example 2 supra) (0.080 g, 0.32 mmol) was added. Mixture was stirred for 18 hours and then partitioned between ethyl acetate and water. The aqueous phase was extracted with ethyl acetate (3×). The combined organic phase was washed with water and brine, dried (magnesium sulfate) and concentrated. The residue was collected by filtration, washed with ethyl acetate and water and dried in vacuum oven to give tert-butyl 3-(4-chloro-3-(7-methoxy-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carboxamido) benzamido)-3-phenylpropylcarbamate. (Yield 0.11 g, 67.8%).
HR-MS (ES+) m/z Calculated for C31H32ClN5NaO6 ([M+Na]+): 628.1933. Found: 628.1955.
Triethylamine (0.044 mL, 0.32 mmol) was added to a mixture of 3-(7-methoxy-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carboxamido)-4-methylbenzoic acid (from Example 93 supra) (0.10 g, 0.28 mmol) and HATU (0.12 g, 0.32 mmol) in DMF (4 mL). The resultant mixture was stirred at room temperature for 30 minutes. (3-Amino-3-phenyl-propyl)-carbamic acid tert-butyl ester (from Example 2 supra) (0.085 g, 0.34 mmol) was added. The mixture was stirred at room temperature for 18 hours and then partitioned between ethyl acetate and water. The aqueous phase was extracted with ethyl acetate (3×). The combined organic phase was washed with water and brine, dried (magnesium sulfate) and concentrated. The residue was collected by filtration, washed with ethyl acetate and water and dried in vacuum oven to give tert-butyl 3-(3-(7-methoxy-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carboxamido)-4-methylbenzamido)-3-phenylpropylcarbamate. (Yield 0.089 g, 53.7%).
HR-MS (ES+) m/z Calculated for C32H35N5NaO6 ([M+Na]+): 608.2479. Found: 608.2477.
Triethylamine (0.022 mL, 0.16 mmol) was added to a mixture of 3-(7-methoxy-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carboxamido)-4-methylbenzoic acid (from Example 93 supra) (0.05 g, 0.14 mmol) and HATU (0.06 g, 0.16 mmol) in DMF (2 mL). Mixture was stirred at room temperature for 30 minutes. Benzylamine (0.018 g, 0.17 mmol) was added. Mixture was stirred at room temperature for 18 hours and then partitioned between ethyl acetate and water. The aqueous phase was extracted with ethyl acetate (3×). The combined organic phase was washed with water and brine, dried (magnesium sulfate) and concentrated. The residue was collected by filtration, washed with ethyl acetate and water and dried in vacuum oven to give N-(5-(benzylcarbamoyl)-2-methylphenyl)-7-methoxy-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carboxamide. (Yield 0.048 g, 76.7%).
HR-MS (ES+) m/z Calculated for C25H22N4NaO4 ([M+Na]+): 465.1533. Found: 465.1534.
Triethylamine (0.022 mL, 0.16 mmol) was added to a mixture of 3-(7-methoxy-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carboxamido)-4-methylbenzoic acid (from Example 93 supra) (0.05 g, 0.14 mmol) and HATU (0.06 g, 0.16 mmol) in DMF (2 mL). The resultant mixture was stirred at room temperature for 30 minutes. 3-Chlorobenzylamine (0.024 g, 0.17 mmol) was added. The mixture was stirred at room temperature for 18 hours and then partitioned between ethyl acetate and water. The aqueous phase was extracted with ethyl acetate (3×). The combined organic phase was washed with water and brine, dried (magnesium sulfate) and concentrated. The residue was collected by filtration, washed with ethyl acetate and water and dried in vacuum oven to give N-(5-(3-chlorobenzylcarbamoyl)-2-methylphenyl)-7-methoxy-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carboxamide. (Yield 0.052 g, 77.1%).
HR-MS (ES+) m/z Calculated for C25H21ClN4NaO4 ([M+Na]+): 499.1143. Found: 499.1143.
To a solution of tert-butyl 3-(3-(7-methoxy-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbox-amido)-4-methylbenzamido)-3-phenylpropylcarbamate (from Example 97 supra) (0.079 g, 0.13 mmol) in dichloromethane (5 mL) was added trifluoroacetic acid (1.0 mL). The mixture was stirred at room temperature for 18 hours. The mixture was concentrated and then partitioned between dichoromethane and saturated sodium carbonate solution. The precipitate was collected by filtration and washed with dichloromethane, methanol and water and dried in vacuum oven to give N-(5-(3-amino-1-phenylpropylcarbamoyl)-2-methylphenyl)-7-methoxy-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carboxamide. (Yield 0.045 g, 69.1%).
HR-MS (ES+) m/z Calculated for C27H28N5O4 ([M+H]+): 486.2136. Found: 486.2138.
To a solution of tert-butyl 3-(4-chloro-3-(7-methoxy-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carboxamido)benzamido)-3-phenylpropylcarbamate (from Example 96 supra) (0.09 g, 0.15 mmol) in dichloromethane (5 mL) was added trifluoroacetic acid (1.0 mL). The mixture was stirred at room temperature for 18 hours. The mixture was concentrated and then partitioned between dichoromethane and saturated sodium carbonate solution. The precipitate was collected by filtration and washed with dichloromethane, methanol and water and dried in vacuum oven to give N-(5-(3-amino-1-phenylpropylcarbamoyl)-2-chlorophenyl)-7-methoxy-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carboxamide. (Yield 0.055 g, 72.3%).
HR-MS (ES+) m/z Calculated for C26H25ClN5O4 ([M+H]+): 506.1590. Found: 506.1589.
The pharmacological properties of the compounds of this invention may be confirmed by a number of pharmacological assays. The exemplified antiproliferative activity assays which follow have been carried out with compounds according to the invention.
If test compounds were assessed in multiple runs of the same assay, the activities reported in Table I are the averages of the results obtained from the multiple runs of the assay.
The kinase TR-FRET (IMAP-Tb) assay uses a fluorescence labeled substrate peptide in the kinase reaction. Upon phosphorylation by the kinase, phosphopeptide is produced, which will be detected by the binding solution provided in IMAP TR-FRET binding kit. After the completion of the kinase reaction, the reaction will be stopped by adding the binding solution containing terbium tracer. This tracer is immobilized on the surface of the IMAP beads, which also contain metal ions on the beads that bind to the phospho-groups of the products. Thus the phosphorylated product of the reaction can enter into close proximity to the tracer, producing resonance energy transfer. Due to the long lifetime of terbium (Tb) fluorescence the detection can be run in time resolved mode, which virtually eliminates fluorescence interference from assay components or compounds.
The TR-FRET signal measurement from this assay, given as an IC50 measurement, is a measure of a test compound's ability to interfere with the phosphorylation of the peptide substrate, that is it inhibits the phosphorylation of the substrate peptide by DYRK1B, and is thus a measure of the test compound's ability to inhibit the activity of DYRK1B. IC50 is the amount of test compound that inhibits 50% of the activity of DYRK1B in this assay. In some cases where the IC50 values were not determined, then the % inhibition at 10 μM test compound concentration may be reported instead. The results of this assay for sample compounds of the invention are provided in Table I below.
TR-FRET Signal=(Reading at 520 nM/Reading at 490 nM)×2000000
The kinase TR-FRET (IMAP-Tb) assay uses a fluorescence labeled substrate peptide in the kinase reaction. Upon phosphorylation by the kinase, phosphopeptide is produced, which will be detected by the binding solution provided in IMAP TR-FRET binding kit. After the completion of the kinase reaction, the reaction will be stopped by adding the binding solution containing terbium tracer. This tracer is immobilized on the surface of the IMAP beads, which also contain metal ions on the beads that bind to the phospho-groups of the products. Thus the phosphorylated product of the reaction can enter into close proximity to the tracer, producing resonance energy transfer. Due to the long lifetime of terbium (Tb) fluorescence the detection can be run in time resolved mode, which virtually eliminates fluorescence interference from assay components or compounds.
The TR-FRET signal measurement from this assay, given as an IC50 measurement, is a measure of a test compound's ability to interfere with the phosphorylation of the peptide substrate, that is it inhibits the phosphorylation of the substrate peptide by DYRK1A, and is thus a measure of the test compound's ability to inhibit the activity of DYRK1A. IC50 is the amount of test compound that inhibits 50% of the activity of DYRK1A in this assay. In some cases where the IC50 values were not determined, then the % inhibition at 10 μM test compound concentration may be reported instead. The results of this assay for sample compounds of the invention are provided in Table I below.
TR-FRET Signal=(Reading at 520 nM/Reading at 490 nM)×2000000
1. Cell Plate Preparation: SW620 human colon cancer cells (known to express DYRK1B), obtained from ATCC, were seeded into 96-well plates at 3×103 cells/well in 50 μL of media.
The results of this assay, given as EC50 values, indicate the concentration of test compound that inhibits tumor cell proliferation by 50%. The results of this assay for sample compounds of the invention are provided in Table I below.
This application claims the benefit of U.S. Provisional Application No. 61/433,999, filed Jan. 19, 2011, which is hereby incorporated by reference in its entirety.
| Number | Date | Country | |
|---|---|---|---|
| 61433999 | Jan 2011 | US |
