2-(1-Piperazinyl)-4-phenylcycloalkanopyridine derivatives, processes for the production thereof, and pharmaceutical composition containing the same

This invention relates to novel 2-(1-piperazinyl)-4-phenylcycloalkanopyridine derivatives having psychotropic activity, processes for the production thereof, and a pharmaceutical composition containing the said compound as an active ingredient.
PRIOR ART
There have hitherto been known some 2-(1-piperazinyl)-4-arylpyridine derivatives and related compounds which have pharmacological activities. For example, U.S. Pat. No. 4,469,696 [Japanese First Publication (Kokai) No. 58-963] discloses 2-(1-piperazinyl)-4-arylpyridine derivatives, however, the compounds disclosed therein are merely the compounds having an aryl group or a 2-furyl group at 5-position of pyridine ring, structure of which is completely different from that of the compounds of the present invention. Besides, the pharmacological activity of these compounds disclosed in the above reference is lipid absorption-inhibitory action which is also completely different from that of the compounds of the present invention. It is clear from the experiments by the present inventors, whereby the 4,5-bis-(4-fluorophenyl)-6-methyl-2-(4-phenyl-1-piperazinyl)pyridine disclosed in the above mentioned U.S. patent did not show any psychotropic activity, that is, it shows no inhibitory effect on an apomorphine-induced vomiting, which can be an index for anti-psychotic drug and no binding property to dopamine (D.sub.2) and serotonin (S.sub.2) receptors.
Moreover, a pyridine derivative having a piperazinyl group and a phenyl group at 2-position and 4-position of pyridine ring thereof respectively, 2-[4-(4-methylbenzyl)-1-piperazinyl]-4-phenylpyridine has been reported to have a weak activity as an anti-psychotic drug or as a neuroleptic drug, [cf: U.S. Pat. No. 4,831,034, Japanese First Publication (Kokai) No. 63-48267]. However, the structure of this compound is obviously different from that of the compounds of the present invention in respect to that this compound does not form the condensed ring between 5- and 6-positions of pyridine ring.
BRIEF DESCRIPTION OF THE INVENTION
The present inventors have extensively searched for compounds having an activity on the central nervous system and have found that novel 2-(1-piperazinyl)-4-phenylcycloalkanopyridine derivatives of the formula (I) disclosed hereinafter have excellent psychotropic activity and are useful as an anti-psychotic drug or an anti-anxiety drug and further as a drug for the treatment of cerebral insufficiency diseases.
An object of the invention is to provide novel 2-(1-piperazinyl)-4-phenylcycloalkanopyridine derivatives having excellent psychotropic activity. Another object of the invention is to provide processes for the production of these compounds. A further object of the invention is to provide a pharmaceutical composition being useful as an anti-psychotic or anti-anxiety drug etc. containing said compound as an active ingredient. These and other objects and advantages of the invention will be apparent to those skilled in the art from the following description.

DETAILED DESCRIPTION OF THE INVENTION
The novel 2-(1-piperazinyl)-4-phenylcycloalkanopyridine derivatives of the present invention have the following formula (I): ##STR2## wherein n is 3, 4, 5, 6 or 7,
R.sup.1 is a hydrogen atom, C.sub.1 -C.sub.10 alkyl, C.sub.2 -C.sub.6 alkenyl, C.sub.2 -C.sub.6 alkynyl, C.sub.3 -C.sub.8 cycloalkyl, C.sub.3 -C.sub.6 cycloalkyl-(C.sub.1 -C.sub.4)alkyl, hydroxy-(C.sub.2 -C.sub.6) alkyl, C.sub.1 -C.sub.3 alkoxy-(C.sub.2 -C.sub.6) alkyl, acyloxy-(C.sub.2 -C.sub.6) alkyl, unsubstituted or substituted aroyl-(C.sub.1 -C.sub.6) alkyl, unsubstituted or substituted aryl, heteroaryl, or acyl,
R.sup.2 and R.sup.3 are the same or different and are each a hydrogen atom, a halogen atom, C.sub.1 -C.sub.6 alkyl, C.sub.1 -C.sub.6 alkoxy, trifluoromethyl, or hydroxy,
R.sup.4, R.sup.5 and R.sup.6 are the same or different and are each a hydrogen atom, C.sub.1 -C.sub.6 alkyl, or phenyl, or two of R.sup.4, R.sup.5 and R.sup.6 combine to form a sigle bond or C.sub.1 -C.sub.3 alkylene,
R.sup.7 and R.sup.8 are the same or different and are each a hydrogen atom or C.sub.1 -C.sub.3 alkyl,
m is 2 or 3,
or an acid addition salt thereof.
The salt of the compounds of the formula (I) includes salts of inorganic acids (e.g. hydrochloride, hydrobromide, hydroiodide, sulfate, phosphate, etc.), and salts of organic acids (e.g. maleate, fumarate, citrate, oxalate, tartrate, lactate, benzoate, methanesulfonate, etc.). Besides, these salts may optionally be present in the form of a hydrate, and hence, the compounds of the present invention include also these hydrate compounds.
Besides, when the compounds of the formula (I) contain asymmetric carbons, these compounds include stereoisomers, a mixture thereof, and a racemic mixture, which are also included as the active compound in the present invention.
In the present specification and claims, the groups in the formulae denote the following groups.
The "alkyl" and "alkyl moiety" and "alkylene" include straight chain or branched chain alkyl groups or alkylene groups.
The "alkyl" includes methyl, ethyl, propyl, isopropyl, butyl, isobutyl, sec-butyl, pentyl, isopentyl, neopentyl, hexyl, isohexyl, heptyl, octyl, nonyl, decyl, and the like.
The "alkylene" includes methylene, ethylene, propylene, and the like.
The "halogen atom" includes fluorine, chlorine, bromine, and iodine.
The "alkoxy" includes methoxy, ethoxy, propoxy, isopropoxy, butoxy, isobutoxy, pentyloxy, hexyloxy, and the like.
The "cycloalkyl" includes, for example, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, and the like.
The "cycloalkyl-alkyl" includes cyclopropylmethyl, cycylobutylmethyl, cyclopentylmethyl, and the like.
The "hydroxyalkyl" includes 2-hydroxyethyl, 2-hydroxypropyl, 3-hydroxypropyl, 2,3-dihydroxypropyl, 4-hydroxybutyl, 2-hydroxybutyl, and the like.
The "alkoxyalkyl" includes methoxymethyl, methoxyethyl, ethoxyethyl, and the like.
The "alkenyl" includes vinyl, allyl, 1-propenyl, isopropenyl, 1-butenyl, 2-butenyl, and the like.
The "alkynyl" includes ethynyl, propargyl, and the like.
The "aryl" includes phenyl, naphthyl, and the like. The "unsubstituted or substituted aryl" includes aryl groups having no substituent or one or two substituents selected from halogen atom, C.sub.1 -C.sub.3 alkyl, C.sub.1 -C.sub.3 alkoxy, and trifluoromethyl, and the examples are phenyl, 4-fluorophenyl, and the like.
The "unsubstituted or substituted aroylalkyl" includes aroylalkyl groups in which the aryl moiety is the above-mentioned unsubstituted or substituted aryl, and the examples are benzoylmethyl, p-fluorobenzoylpropyl, and the like.
The "heteroaryl" means a monocyclic or bicyclic heterocyclic group containing at least one hetero atom selected from nitrogen, oxygen and sulfur, for example, furyl, thienyl, pyridyl, pyrimidyl, isoquinolyl, and the like.
The "acyl" includes C.sub.1 -C.sub.4 alkanoyl, C.sub.5 -C.sub.6 cycloalkylcarbonyl, a benzoyl group which may optionally be substituted by halogen, C.sub.1 -C.sub.3 alkyl or C.sub.1 -C.sub.3 alkoxy, a heteroarylcarbonyl group in which the heteroaryl moiety is the above-mentioned heteroaryl, and the examples are formyl, acetyl, propionyl, butyryl, cyclohexanecarbonyl, benzoyl, benzoyl, nicotinoyl, isonicotinoyl, 4-fluorobenzoyl, furoyl, thenoyl, and the like.
The "acyloxyalkyl" includes acyloxyalkyl groups in which the acyl moiety is the above-mentioned acyl, and the examples are 2-acetyloxyethyl, 3-acetoxypropyl, benzoyloxyethyl, and the like.
Among the compounds of the formula (I) of the present invention, preferred compounds are those of the formula (I) wherein R.sup.1 is a hydrogen atom, C.sub.1 -C.sub.10 alkyl, C.sub.3 -C.sub.8 cycloalkyl, hydroxy-(C.sub.2 -C.sub.6) alkyl, C.sub.1 -C.sub.3 alkoxy-(C.sub.2 -C.sub.4) alkyl, C.sub.2 -C.sub.4 alkanoyloxy-(C.sub.2 -C.sub.6) alkyl, a benzoyl-(C.sub.2 -C.sub.5) alkyl group in which the phenyl moiety may optionally be substituted by halogen, C.sub.1 -C.sub.3 alkyl or C.sub.1 -C.sub.3 alkoxy, C.sub.2 -C.sub.4 alkenyl, C.sub.2 -C.sub.4 alkynyl, pyridyl, pyrimidyl, C.sub.2 -C.sub.5 alkanoyl or furoyl, R.sup.2 and R.sup.3 are the same or different and are each hydrogen atom, halogen atom, methyl or methoxy, R.sup.4, R.sup.5 and R.sup.6 are the same or different and are each a hydrogen atom or C.sub.1 -C.sub.4 alkyl, or two of them combine to form C.sub.1 -C.sub.2 alkylene, R.sup.7 and R.sup.8 are the same or different, and are each hydrogen atom or C.sub.1 -C.sub.3 alkyl, and m is 2, and an acid addition salt thereof.
Further preferred compounds of the present invention are the compounds of the formula (I) wherein R.sup.1 is a hydrogen atom, C.sub.1 -C.sub.8 alkyl, C.sub.3 -C.sub.8 cycloalkyl, hydroxy-(C.sub.2 -C.sub.6)alkyl, C.sub.1 -C.sub.2 alkoxy-(C.sub.2 -C.sub.3) alkyl, acetyloxy-(C.sub.2 -C.sub.4) alkyl, C.sub.3 -C.sub.4 alkenyl, or C.sub.2 -C.sub.3 alkanoyl, R.sup.2 and R.sup.3 are the same and both are hydrogen atom or halogen atom, or one of them is hydrogen atom and the other is halogen atom, methyl, or methoxy, and an acid addition salt thereof. More preferred compounds of the present invention are the compounds of the formula (I) wherein R.sup.2 and R.sup.3 are the same or different and are each a hydrogen atom or a fluorine atom, and an acid addition salt thereof.
Particularly preferred compounds are the compounds of the following formula (I-1): ##STR3## wherein n.sub.1 is 3, 6 or 7,
R.sup.11 is hydrogen atom, C.sub.1 -C.sub.8 alkyl, C.sub.3 -C.sub.6 cycloalkyl, hydroxy-(C.sub.2 -C.sub.4) alkyl, C.sub.1 -C.sub.2 alkoxy-(C.sub.2 -C.sub.3) alkyl, or C.sub.3 alkenyl,
R.sup.21 and R.sup.31 are the same or different and are each a hydrogen atom or a fluorine atom,
R.sup.71 and R.sup.81 are the same or different and are each a hydrogen atom or C.sub.1 -C.sub.3 alkyl, or an acid addition salt thereof, and of the following formula (I-2): ##STR4## wherein n.sub.2 is 4 or 5,
R.sup.12 is a hydrogen atom, C.sub.1 -C.sub.8 alkyl, C.sub.3 -C.sub.8 cycloalkyl, hydroxy-(C.sub.2 -C.sub.4) alkyl, C.sub.1 -C.sub.2 alkoxy-(C.sub.2 -C.sub.3) alkyl or C.sub.3 alkenyl,
R.sup.22 and R.sup.32 are the same or different and are each a hydrogen atom or a fluorine atom,
R.sup.42, R.sup.52 and R.sup.62 are the same or different and are each a hydrogen atom or C.sub.1 -C.sub.4 alkyl, or two of them combine to form C.sub.1 -C.sub.2 alkylene, or an acid addition salt thereof.
Especially most preferred compounds in the present invention are the compounds of the formula (I-1) wherein n.sub.1 is 6, R.sup.11 is C.sub.1 -C.sub.6 alkyl, C.sub.3 -C.sub.6 cycloalkyl, or hydroxy-(C.sub.2 -C.sub.4)alkyl, R.sup.21 and R.sup.31 are the same or different and are each a hydrogen atom or a fluorine atom substituted at 2- or 4-position, R.sup.71 and R.sup.81 are each a hydrogen atom, and a salt thereof, and the compounds of the formula (I-2) wherein n.sub.2 is 5, R.sup.12 is C.sub.1 -C.sub.6 alkyl, C.sub.3 -C.sub.6 cycloalkyl or hydroxy-(C.sub.2 -C.sub.4) alkyl, R.sup.22 and R.sup.32 are the same or different and are each a hydrogen atom or a fluorine atom substituted at 2- or 4-position, R.sup.42, R.sup.52 and R.sup.62 are all hydrogen atoms, or two of them combine to form C.sub.1 -C.sub.2 alkylene, and another one is a hydrogen atom, and an acid addition salt thereof.
The most preferred compounds of the present invention are the compounds of the following formula (I-3): ##STR5## wherein R.sup.13 is methyl, ethyl, propyl, butyl, pentyl or hydroxyethyl, and R.sup.33 is a hydrogen atom or a fluorine atom, and a salt thereof.
Specifically preferred compounds of the present invention are as follows.
2-(4-Methyl-1-piperazinyl)-4-(4-fluorophenyl)-5,6,7,8,9,10-hexahydrocycloocta[b]pyridine
2-(4-Methyl-1-piperazinyl)-4-(2,4-difluorophenyl)-5,6,7,8,9,10-hexahydrocycloocta[b]pyridine
2-(4-Ethyl-1-piperazinyl)-4-(4-fluorophenyl)-5,6,7,8,9,10-hexahydrocycloocta[b]pyridine
2-(4-Ethyl-1-piperazinyl)-4-(2,4-difluorophenyl)-5,6,7,8,9,10-hexahydrocycloocta[b]pyridine
2-(4-n-Propyl-1-piperazinyl)-4-(4-fluorophenyl)-5,6,7,8,9,10-hexahydrocycloocta[b]pyridine
2-(4-n-Propyl-1-piperazinyl)-4-(2,4-difluorophenyl)-5,6,7,8,9,10-hexahydrocycloocta[b]pyridine
2-(4-n-Butyl-1-piperazinyl)-4-(4-fluorophenyl)-5,6,7,8,9,10-hexahydrocycloocta[b]pyridine
2-(4-n-Pentyl-1-piperazinyl)-4-(4-fluorophenyl)-5,6,7,8,9,10-hexahydrocycloocta[b]pyridine
2-[4-(2-Hydroxyethyl)-1-piperazinyl]-4-(4-fluorophenyl)-5,6,7,8,9,10-hexahydrocycloocta[b]pyridine
The compounds of the present invention can be prepared, for example, by the following processes.
(1) Process A
The compounds of the formula (I) are prepared by reacting a compound of the formula (II): ##STR6## wherein X is a leaving atom or group, and R.sup.2, R.sup.3, R.sup.4, R.sup.5, R.sup.6 and n are as defined above, with a compound of the formula (III): ##STR7## wherein R.sup.1, R.sup.7, R.sup.8 and m are as defined above.
The leaving atom or group X in the formula (II) denotes any atom or group which can leave off in the form of HX under the reaction conditions together with the hydrogen atom bonded to the nitrogen atom at 4-position of 1-substituted piperazines or homopiperazines. Examples of the leaving atom or group are halogen atoms, lower alkythio groups (e.g. methylthio, ethylthio, propylthio, butylthio, etc.), arylsulfonyloxy groups (e.g. benzenesulfonyloxy, p-toluenesulfonyloxy, etc.), and alkylsulfonyloxy groups (e.g. methanesulfonyloxy, etc.).
The reaction of the compound of the formula (II) and the compound of the formula (III) is carried out in an appropriate solvent or without using any solvent under atmospheric pressure or under pressure. Suitable examples of the solvent are aromatic hydrocarbons (e.g. toluene, xylene, etc.), ketones (e.g. methyl ethyl ketone, etc.), ethers (e.g. dioxane, diglyme, etc.), alcohols (e.g. ethanol, isopropyl alcohol, butanol, etc.), N,N-dimethylformamide, dimethylsulfoxide. The reaction is preferably carried out in the presence of a basic substance. Suitable examples of the basic substance are alkali metal carbonates (e.g. sodium carbonate, potassium carbonate, etc.), alkali metal hydrogen carbonates (e.g. sodium hydrogen carbonate, potassium hydrogen carbonate, etc.), tertiary amines (e.g. triethylamine, etc.), but an excess amount of the compound of the formula (III) may be used instead of using the basic substance. When the compound of the formula (III) is in the form of a hydrate, the hydrate may be used. The reaction temperature is usually in the range of 40.degree. to 200.degree. C. The starting compound (II) can be prepared in the procedure as described in Reference Examples 1 to 94 hereinafter or in a similar process.
(2) Process B
The compounds of the formula (I) wherein R.sup.1 is a hydrogen atom can be prepared by hydrogenolysis of a compound of the formula (I-4): ##STR8## wherein R.sup.14 is unsubstituted or substituted benzyl or benzyloxycarbonyl (the substituent on the phenyl ring of said benzyl and benzyloxycarbonyl is a member selected from lower alkyl, lower alkoxy and halogen atoms), and R.sup.2, R.sup.3, R.sup.4, R.sup.5, R.sup.6, R.sup.7, R.sup.8, m and n are as defined above.
The hydrogenolysis of the compounds of the formula (I-4) is usually carried out by a conventional catalytic reduction in a solvent such as alcohols (e.g. ethanol, etc.) at room temperature under atmospheric pressure. The compounds of the formula (I-4) can be prepared by the same process as the above-mentioned Process A. The compounds of the formula (I-4) wherein R.sup.14 is benzyloxycarbonyl can alternatively be prepared by a conventional process from a compound of the formula (I) wherein R.sup.14 is methyl or benzyl having optionally a substituent.
(3) Process C
The compounds of the formula (I) wherein R.sup.1 is a hydrogen atom can also be prepared by reacting a compound of the formula (I-5): ##STR9## wherein R.sup.15 is methyl or unsubstituted or substituted benzyl (the substituent on the phenyl ring of the benzyl is a member selected from lower alkyl, lower alkoxy and halogen atoms), and R.sup.2, R.sup.3, R.sup.4, R.sup.5, R.sup.6, R.sup.7, R.sup.8, m and n are as defined above, with ethyl chlorocarbonate or 1-chloroethyl chlorocarbonate to give a compound of the formula (I-6): ##STR10## wherein R.sup.16 is ethoxycarbonyl, 1-chloroethoxycarbonyl, and R.sup.2, R.sup.3, R.sup.4, R.sup.5, R.sup.6, R.sup.7, R.sup.8, m and n are as defined above, or a compound of the formula (I-7): ##STR11## wherein R.sup.17 is acyl, and R.sup.2, R.sup.3, R.sup.4, R.sup.5, R.sup.6, R.sup.7, R.sup.8, m and n are as defined above, followed by hydrolysis of the compound of the formula (I-6) or (I-7).
The hydrolysis of the compound of the formula (I-6) or (I-7) is usually carried out by a conventional method, for example, by heating the compound in an appropriate solvent such as ethanol which is miscible with water in the presence of a basic substance (e.g. sodium hydroxide, potassium hydroxide, etc.) or an acid (e.g. hydrochloric acid, sulfuric acid, etc.). The hydrolysis of the compound of the formula (I-6) wherein R.sup.16 is 1-chloroethoxycarbonyl is usually carried out by heating the compound in methanol. The compounds of the formulae (I-5) and (I-7) can be prepared by the same process as the above-mentioned Process A.
(4) Process D
The compound of the formula (I) wherein R.sup.1 is a group other than a hydrogen atom can be prepared by reacting a compound of the formula (I-8): ##STR12## wherein R.sup.2, R.sup.3, R.sup.4, R.sup.5, R.sup.6, R.sup.7, R.sup.8, m and n are as defined above, with a compound of the formula (IV):
R.sup.18 --Z (IV)
wherein Z is a reactive residue of an alcohol, and R.sup.18 is the same as R.sup.1 except a hydrogen atom.
The reactive residue of an alcohol represented by the group Z includes, for example, a halogen atom (e.g. chlorine, bromine, iodine, etc.), lower alkylsulfonyloxy (e.g. methanesulfonyloxy, etc.), arylsulfonyloxy (e.g. benzenesulfonyloxy, p-toluenesulfonyloxy, etc.).
The reaction of the compound of the formula (I-8) and the compound of the formula (IV) is usually carried out in an appropriate solvent. Suitable examples of the solvent are aromatic hydrocarbons (e.g. benzene, xylene, etc.), ketones (e.g. methyl ethyl ketone, etc.), ethers (e.g. dioxane, etc.), N,N-dimethylformamide. The reaction is preferably carried out in the presence of a basic substance. The basic substance includes the same substances as used in the above Process A. The reaction temperature is usually in the range of 30.degree. to 150.degree. C. The compounds of the formula (I-8) can be prepared by the same processes as the above-mentioned Processes A to C.
When the compounds of the formula (I) prepared by the Processes A and D have a hydroxyl group in the structure thereof, they can be converted into corresponding ester derivatives or ether derivatives by reacting the compound with an appropriate acylating agent or a lower alkylating agent. These reactions are usually carried out by a conventional method.
The compounds of the formula (I) prepared by the above mentioned Processes can be isolated and purified from the reaction mixture by a conventional method.
The compounds of the formula (I) of the present invention are obtained in the form of a free base or a salt or a hydrate depending on the kinds of the starting compound, the kinds of reaction, the reaction conditions, and the like. When the compounds are obtained in the form of a salt, they can be converted into the corresponding free base by a conventional method, for example, by treating them with a basic substance such as an alkali metal hydroxide. Besides, when the compounds are obtained in the form of a free base, they can be converted into the corresponding salt by a conventional method, for example, by treating them with various acids.
The compounds of the formula (I) of the present invention show inhibitory effect on exploratory activity, antagonistic effect on apomorphine-induced vomiting, binding property to dopamine (D.sub.2) and serotonin (S.sub.2) receptors, increasing effect on concentration of brain monoamine metabolites, and little toxicity. Accordingly, the compounds of the present invention are useful as an anti-psychotic (neuroleptic) drug or an anti-anxiety (anxiolytic) drug.
Furthermore, the compounds of the formula (I) of the present invention show excellent improving effect in some animal models of memory impairment. Accordingly, the compounds of the present invention are also useful as a medicament for the treatment of various symptoms of cerebral insufficiency.
The compounds of the present invention which show potent antagonistic effect on apomorphine-induced vomiting, binding property to serotonin (S.sub.2) and dopamine (D.sub.2) receptors, and increasing effect on concentration of brain monoamine metabolites are, for example, the following compounds and a pharmaceutically acceptable salt thereof.
(1) 2-(4-Ethyl-1-piperazinyl)-4-(4-fluorophenyl)-5,6,7,8,9,10-hexahydrocycloocta[b]pyridine
(2) 2-(4-Ethyl-1-piperazinyl)-4-(2,4-difluorophenyl)-5,6,7,8,9,10-hexahydrocycloocta[b]pyridine
(3) 2-(4-Methyl-1-piperazinyl)-4-(4-fluorophenyl)-5,6,7,8,9,10-hexahydrocycloocta[b]pyridine
(4) 2-(4-Methyl-1-piperazinyl)-4-(2,4-difluorophenyl)-5,6,7,8,9,10-hexahydrocycloocta[b]pyridine
(5) 2-(4-n-Propyl-1-piperazinyl)-4-(4-fluorophenyl)-5,6,7,8,9,10-hexahydrocycloocta[b]pyridine
(6) 2-(4-n-Butyl-1-piperazinyl)-4-(4-fluorophenyl)-5,6,7,8,9,10-hexahydrocycloocta[b]pyridine
(7) 2-(4-n-Pentyl-1-piperazinyl)-4-(4-fluorophenyl)-5,6,7,8,9,10-hexahydrocycloocta[b]pyridine
(8) 2-[4-(2-Hydroxyethyl)-1-piperazinyl]-4-(4-fluorophenyl)-5,6,7,8,9,10-hexahydrocycloocta[b]pyridine
(9) 2-(1-Piperazinyl)-4-(4-fluorophenyl)-6,7-dihydro-5H-1-pyrindine
(10) 2-(4-Ethyl-1-piperazinyl)-4-(4-fluorophenyl)-6,7-dihydro-5H-1-pyrindine
(11) 2-[4-(2-Hydroxyethyl)-1-piperazinyl]-4-(4-fluoro-phenyl)-6,7-dihydro-5H-1-pyrindine
(12) 2-(4-Ethyl-1-piperazinyl)-4-phenyl-6,7,8,9-tetrahydro-5H-cyclohepta[b]pyridine
(13) 2-(4-Ethyl-1-piperazinyl)-4-(4-fluorophenyl)-6,7,8,9-tetrahydro-5H-cyclohepta[b]pyridine
(14) 2-(4-Ethyl-1-piperazinyl)-4-(4-fluorophenyl)-5,6,7,8-tetrahydro-5,8-methanoquinoline
(15) 2-(4-Ethyl-1-piperazinyl)-4-(2,4-difluorophenyl)-5,6,7,8-tetrahydro-5,8-methanoquinoline
(16) 2-(4-Ethyl-1-piperazinyl)-4-(4-fluorophenyl)-6,7,8,9-tetrahydro-5H-6,9-methanocyclohepta[b]pyridine
(17) 2-(4-Ethyl-1-piperazinyl)-4-(4-fluoro-phenyl)-6,7,8,9- tetrahydro-5H-5,8-methanocyclohepta[b]pyridine
The compounds of the present invention which have excellent improving effects on behavioral and/or memory deficites induced by scopolamine or cycloheximide are, for example, the following compounds and a pharmaceutically acceptable salt thereof.
(1) 2-(1-Piperazinyl)-4-phenyl-6,7-dihydro-5H-1-pyrindine
(2) 2-(1-Piperazinyl)-4-(4-fluorophenyl)-6,7-dihydro-5H-1-pyridine
(3) 2-(1-Piperazinyl)-4-phenyl-5,6,7,8-tetrahydroquinoline
(4) 2-(1-Piperazinyl)-4-(4-fluorophenyl)-5,6,7,8-tetrahydroquinoline
(5) 2-(1-Piperazinyl)-4-(4-fluorophenyl)-6,7,8,9-tetrahydro-5H-cyclohepta[b]pyridine
The pharmacological activities of the representative compounds of the present invention are illustrated by the following Experiments.
In the Experiments, the following compounds, which are disclosed in the above mentioned U.S. Pat. No. 4,469,696, were used as reference.
Test Compound A (disclosed in Example 4):
4,5-Bis-(4-fluorophenyl)-6-methyl-2-(4-phenylpiperazino)pyridine
Test Compound B (disclosed in Example 1):
4,5-Bis-(4-methoxyphenyl)-6-methyl-2-(4-phenylpiperazino)pyridine
Test Compound C (disclosed in Example 2):
4,5-Bis-(4-hydroxyphenyl)-6-methyl-2-(4-phenylpiperazino)pyridine
EXPERIMENT 1
Inhibitory Effect on Exploratory Activity
A group of 5 male mice (Std-ddy strain, 20-25 g) was used. Two hours after oral administration of the test compound, mice were placed individually in a test box (23.times.35.times.30 cm) on Animex activity meter (Farad Co.). Immediately thereafter, activity counting was started and lasted for three minutes. The mean counts of the compound-treated group were compared with those of the corresponding control (non-dosed) group, and the percent inhibition was calculated. The results are shown in Table 1.
TABLE 1______________________________________Inhibitory effect on exploratory activityTest Inhibitory Test Inhibitorycompound ratio (%) compound ratio (%)______________________________________Ex.1b* 83.7 83 53.52a 77.5 106 74.931 62.5 107 53.236 68.4 111 74.038 58.9 112 73.253 68.1 114 81.957 77.2 122 81.371 61.9 123 72.275 78.9 125 57.976 87.6 127 80.3______________________________________ *The compound of Example 1b (hereinafter, the same)
EXPERIMENT 2
Antagonistic Effect on Apomorphine-induced Vomiting:
A group of 3-4 dogs (Beagle, 8-15 kg) was used for examining effects on the test compounds on apomorphine-induced vomiting, a known test for evaluating neuroleptic drugs.
Each dog was given a subcutaneous injection of apomorphine hydrochloride (0.3 mg/kg) two hours after the treatment of the test compounds. Then, the frequency of oral vomiting was counted for one hour. The mean counts of the compound-treated group were compared with those of the corresponding control (non-dosed) group, and the percent inhibition was calculated. The results are shown in Table 2.
TABLE 2______________________________________Antagonistic effect on apomorphine-inducedvomitingTest Dose Inhibitory Test Dose Inhibitorycomp. (mg/kg) ratio (%) comp. (mg/kg) ratio (%)______________________________________Ex. Ex.1a.sup.1 0.2 100 73 1.0 1001b 0.3 88 76 0.3 712b 0.2 100 77 0.5 616 1.0 89 82 1.0 1007 1.0 80 88 3.0 9415 3.0 100 112 0.2 9425 3.0 100 114 0.2 8927 3.0 47 116 1.0 10029 3.0 63 118 0.3 9436 0.5 81 121 0.5 9337 0.3 80 122 0.5 10043 1.0 96 135 1.0 10056 0.5 85 A.sup.2 3.0 1166 1.0 95 B.sup.2 3.0 1371 3.0 84 C.sup.2 3.0 16______________________________________ .sup.1 The compound of Example 1a (hereinafter, the same) .sup.2 The compounds disclosed in the U.S. Pat. No. 4,469,696
EXPERIMENT 3
In Vitro Binding Property to Dopamine (D.sub.2), Serotonin (S.sub.1, S.sub.2) and Adrenaline (.alpha..sub.1) Receptors:
Dopamine (D.sub.2), serotonin (S.sub.1, S.sub.2) and adrenaline (.alpha..sub.1) receptor binding assays were carried out according to the methods of I. Creese et al. [Eur. J. Pharmacol., 46, 377 (1977), S. J. Peroutka et al. [Mol. Pharmacol. 16, 687 (1979)], J. E. Leysen et al. [Mol. Pharmacol., 21, 301 (1982)] and D. C. U'Prichard et al. [Mol. Pharmacol., 13, 454 (1977)], respectively.
Crude synaptosome fractions were prepared from some brain regions in rats for receptor sources. Radioactive ligands used were [.sup.3 H] spiperone (D.sub.2), [.sup.3 H] serotonin (S.sub.1), [.sup.3 H] ketanserin (S.sub.2) and [.sup.3 H] WB-4101 (.alpha..sub.1). The binding assay was performed by incubating aliquots of synaptosome fraction in buffer solution (final volume: 1 ml) containing a [.sup.3 H] labelled ligand and a test compound. The assay was terminated by rapid filtration through Whatman GF/B glass fiber filters attached to a cell-harvester (Brandel) and radioactivity on the filters was counted in a Packard Triscarb scintillation counter. Specific binding was calculated as a difference between amounts of radioactivity in the presence and absence of an unlabelled ligand [spiperone (D.sub.2), serotonin (S.sub.1), methysergide (S.sub.2) and prazosin (.alpha..sub.1)]. The IC.sub.50 value of the test compounds (concentration causing 50% inhibition of [.sup.3 H] ligand specific binding) was determined by probit analysis. The results are shown in Table 3.
TABLE 3______________________________________Binding property to dopamine (D.sub.2),sertonin (S.sub.1, S.sub.2) and adrenaline (.alpha..sub.1)receptorTest IC.sub.50 (nM)compound D.sub.2 S.sub.1 S.sub.2 .alpha..sub.1______________________________________Ex.1a.sup.1 24 -- 9.9 831b 46 -- 7.0 922a 24 -- 28 886 19 1500 7.6 6.97 30 -- 9.5 --15 58 1700 19 3523 96 2900 7.7 32025 77 2600 2.3 12071 25 -- 14 1373 18 -- 2.4 4.686 730 1400 81 53087 270 820 28 9488 70 670 9.1 6592 300 1900 27 38096 660 4900 62 110097 260 4100 5.1 890103 140 -- 41 --A.sup.2 >10000 -- >10000 >10000B.sup.2 >10000 -- -- --C.sup.2 >10000 -- -- --______________________________________ .sup.1 The compound of Example 1a (hereinafter, the same) .sup.2 The compounds disclosed in the U.S. Pat. No. 4,469,696
EXPERIMENT 4
Increasing Effect on Concentration of Brain Monoamine Metabolites
A group of 5 male mice (Std-ddy strain, 25-30 g) was used for examining effect of the test compounds on concentration of brain monoamine metabolites. It is generally accepted that an increase in each monoamine is mainly caused by each monoamine receptor blockade.
Mice were killed by decapitation 2 hours after treatment with the test compounds. Brains were quickly taken out, homogenized in 1N formic acid-acetone solution, and centrifuged in a refrigerated ultracentrifuge. The supernatant was evaporated by blowing with N.sub.2 gas. Then, the residue was again resolved in 0.01 N acetic acid, and served for determining concentration of dopamine metabolites, 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA), a norepinephrine metabolite, 3-methyl-4-hydroxyphenylethylene glycol (MOPEG), and a serotonin metabolite, 5-hydroxyindole-3-acetic acid (5-HIAA) concentrations by HPLC with electrochemical detection. The effect of the test compounds on concentration of each monoamine metabolite is shown as % of control (level of non-dosed animals=100) in Table 4.
TABLE 4______________________________________Increasing effect on the concentration ofmonoamine metabolitesTest Dose (%)compound (mg/kg) DOPAC HVA MOPEG 5-HIAA______________________________________EX.1b* 1 235 212 118 1022b 2 364 271 -- --6 10 321 242 115 10315 10 277 235 118 10823 10 344 320 115 14524 10 290 317 139 15025 1 272 207 106 11326 10 344 263 -- --27 10 208 193 -- --29 10 296 274 -- --35 10 165 170 -- --37 3 268 203 143 9539 10 307 233 117 9742 10 258 211 -- --44 10 272 210 -- --46 10 296 235 -- --49 10 197 195 -- --52 10 233 216 -- --54 2 251 250 -- --55 2 379 240 -- --56 10 302 249 106 11059 2 246 236 126 10666 2 311 308 111 11668 2 206 181 -- --84 10 237 216 -- --87 10 180 194 117 13688 10 329 209 136 12092 10 208 192 121 13996 10 201 194 115 12797 10 232 176 125 128104 10 344 247 160 97117 10 242 210 133 100118 3 302 250 129 110121 10 312 215 -- 111122 2 306 229 -- 100124 10 292 238 102 107131 1 319 224 -- --132 10 333 209 -- --138 2 236 173 -- --139 10 267 206 -- --______________________________________ *The compound of Example 1b (hereinafter, the same)
EXPERIMENT 5
Improving Effect on Scopolamine-induced Deficit of Spontaneous Alternation Behavior
A group of 15-25 male mice (Std-ddY strain, 22-28 g) was used for evaluating effect of the test compounds on scopolamine-induced deficit of spontaneous alternation in a T-maze, which is a known animal model of memory impairment due to hypofunction of the cholinergic nervous system. The T-maze used consists of a stem and two arms which are 25 cm long, 5 cm wide and 10 cm high. The first 10 cm of the stem and last 10 cm of each arm are divided by sliding doors into start and goal boxes.
A test compound and scopolamine hydrobromide (1 mg/kg) were intraperitoneally administered to each mouse, and after 30 minutes a test of spontaneous alternation task in the T-maze was continuously repeated for 8 trials. Commonly, naive mice alternate each (right and left) goal box in turn, but scopolamine-treated animals tend to enter the same goal box repeatedly. The effect of the test compounds was expressed as % improvement (complete improvement to the alternation level of non-dosed mice=100%). The results are shown in Table 5.
TABLE 5______________________________________Improving effect on scopolamine-induceddeficit of spontaneous alternation behaviorTest Dose Improvementcompound (mg/kg) (%)______________________________________Ex. 86* 10.0 47.687 0.5 48.0 2.0 60.088 10.0 37.097 10.0 32.0______________________________________ *The compound of Example 86 (hereinafter the same)
EXPERIMENT 6
Improving Effect on Cycloheximide-induced Amnesia of Passive Avoidance Response
Anti-amnesic effect of the test compounds was examined using mice given cycloheximide, which is a known amnesia-inducing agent.
A group of 15-20 male mice (Std-ddY strain, 27-33 g) was subjected to training and retention trials for a passive avoidance task in a step-down apparatus (30.times.30.times.50 cm) with a grid floor and a wooden platform (4.times.4.times.4 cm) in a center of the floor. In the training trial, each mouse was first placed on the platform. When the mouse stepped down on the grid floor, an electric shock (1 Hz, 0.5 sec, 60 VDC) was delivered to the feet for 15 seconds. Immediately after the training trial, cycloheximide (60 mg/kg, s.c.) and a test compound (i.p.) were administered. The retention trial was carried out 24 hours thereafter, the time from placing again each mouse placed on the platform until stepping down on the grid floor (step-down latency) was measured. The step-down latency in the retention trial was markedly shortened by treatment of cycloheximide (amnesia). The effect of test compounds was assessed by % improvement (complete improvement to the latency level of non-dosed animals=100%). The results are shown in Table 6.
TABLE 6______________________________________Improving effect on cycloheximide-inducedamnesia of passive avoidance responseTest Dose Improvementcompound (mg/kg) (%)______________________________________Ex. 87* 0.5 48 2.0 7788 2.0 6092 2.0 44 10.0 7896 0.5 81 2.0 6497 0.5 38 2.0 36______________________________________ *The compound of Example 87 (hereinafter the same)
EXPERIMENT 7
Acute Toxicity
A group of 5 male mice (Std-ddy strain, 25-30 g) was used. The test compound was orally administered to the test animal in the form of a 0.5% tragacanth solution or suspension, and for 7 days after the administration of the test compound, the lethality of animals was observed. The results are shown in Table 7.
TABLE 7______________________________________Acute toxicityTest Dose Number of dead animalscompound (mg/kg) Number of test animals______________________________________Ex.1a* 500 0/51b 500 0/52b 500 0/56 500 0/525 500 0/576 500 0/5______________________________________ *The compound of Example 1a (hereinafter, the same)
The compounds of the present invention can be administered either in oral route, parenteral route or intrarectal route, but preferably in oral route. The dose of the compounds may vary depending on the kinds of the compounds, administration routes, severity of the disease and age of patients, but is usually in the range of 0.01 to 50 mg/kg/day, preferably 0.01 to 5 mg/kg/day.
The compounds of the present invention are usually administered in the form of a conventional pharmaceutical preparation in admixture with a conventional pharmaceutically acceptable carrier or diluent. The pharmaceutically acceptable carrier or diluent includes the conventional pharmaceutically acceptable carriers or diluents which do not react with the compounds of the present invention. Suitable examples of the carrier or diluent are lactose, glucose, mannitol, sorbitol, dextrin, cyclodextrin, starch, sucrose, magnesium metasilicate aluminate, synthetic aluminum silicate, crystalline cellulose, sodium carboxymethyl cellulose, hydroxypropyl starch, calcium carboxymethyl cellulose, ion exchange resin, methyl cellulose, gelatin, acacia, pullulan, hydroxypropyl cellulose, low substituted hydroxypropyl cellulose, hydroxypropyl methyl cellulose, polyvinylpyrrolidone, polyvinyl alcohol, light silicic anhydride, magnesium stearate, talc, tragacanth, bentonite, veegum, carboxyvinyl polymer, titanium oxide, sorbitan fatty acid ester, sodium laurylsulfate, glycerin, glycerin fatty acid ester, anhydrous lanolin, glycerogelatin, polysorbate, macrogol, vegetable oil, wax, propylene glycol, water, and the like. The pharmaceutical preparation includes tablets, capsules, granules, fine granules, powders, syrups, suspensions, injections, suppositories, and the like. These preparations can be prepared by a conventional method. The liquid preparations may be in the form that they are dissolved or suspended in water or any other conventional medium when used. The tablets, granules and fine granules may be coated with a conventional coating agent. The injections are usually prepared by dissolving the compound of the present invention in water, but occasionally in a physiological saline solution or glucose solution, which is optionally incorporated with a buffer or a preservative. The pharmaceutical preparations may also contain other pharmaceutically active compounds.
The present invention is illustrated by the following Reference Examples, Examples and Preparations, but should not be construed to be limited thereto. The compounds are identified by elementary analysis, mass spectrum, IR spectrum, UV spectrum, NMR spectrum, and the like.
In the Reference Examples and Examples, the following abbreviations may occasionally be used.
Me: methyl
Et: ethyl
t-Bu: tertiary butyl
Ph: phenyl
A: ethanol
AC: acetonitrile
AT: acetone
CF: chloroform
D: N,N-dimethylformamide
E: diethyl ether
EA: ethyl acetate
HX: hexane
IP: isopropyl alcohol
M: methanol
MC: methylene chloride
PE: petroleum ether
T: toluene
W: water
Besides, the solvent shown in brackets as to the melting point in the following Reference Examples and Examples means a solvent for recrystallization.
REFERENCE EXAMPLE 1
Preparation of 4-phenyl-5,6,7,8-tetrahydro-2(1H)-quinolinone
A mixture of benzoylacetonitrile (25 g), cyclohexanone (25 g) and 75% polyphosphoric acid (250 g) is stirred at 50.degree. C. for 30 minutes and further at 110.degree. C. for 1.5 hour. After cooling, the reaction mixture is poured into ice-water and thereto is added diethyl ether (300 ml). The mixture is stirred and the precipitated crystals are collected by filtration. The crystals are recrystallized from N,N-dimethylformamide-ethanol to give the desired compound (27 g), m.p. 285.degree.-288.degree. C.
REFERENCE EXAMPLES 2 TO 46
In the same manner as described in Reference Example 1 except that the corresponding starting materials are used, there are obtained the compounds as shown in the following Tables 8 and 9.
TABLE 8______________________________________ ##STR13##Ref. Melting point Solvent forEx. n R.sup.2 R.sup.3 (.degree.C.) recrystallization______________________________________ 2 3 H H 221-223 M 3 3 4-F H 258-265 M 4 3 3-F H 234-235 A 5 3 2-F H 210-212 A-W 6 3 4-OMe H 243-245 M 7 3 4-F 2-F 235-245 A 8 4 4-F H 287-288 D-A 9 4 3-F H 253-255 A10 4 2-F H 174-176 M11 4 4-OMe H 239-242 M12 5 H H 280-281 M13 5 4-F H 245-246 M14 5 3-F H 273-275 M15 5 2-F H 251-252 A-W16 5 4-OMe H 258-260 M17 5 2-OMe H 245-247 A18 5 4-Me H 253-258 M19 5 4-Cl H 251-255 D-W20 5 2-Cl H 262-263 D-W21 5 4-F 2-F 233-235 A22 6 H H 265-266 M23 6 4-F H 235-238 IP24 6 3-F H 260-270 A25 6 2-F H 245-247 IP26 6 4-F 3-F 255-261 M27 6 4-F 2-F 240-243 M28 6 6-F 2-F 254-256 A29 6 4-OMe H 267-271 M30 6 4-Me H 265-275 A31 6 4-Cl H 284-287 M32 6 3-Cl H 248-250 A33 6 4-Br H 286-292 M34 7 4-F H 241-243 EA______________________________________
TABLE 9______________________________________ ##STR14## Ex.Ref. ##STR15## R.sup.3 R.sup.3 (.degree.C.)pointMelting recrystallizationSolvent______________________________________ for35 ##STR16## H H 275-279 M36 ##STR17## F H 270-275 M37 ##STR18## F H 255-257 M38 ##STR19## F H 278-284 M39 ##STR20## F H 266-267 M40 ##STR21## F H -- --41 ##STR22## H H 272-276 M42 ##STR23## F H 273-277 M43 ##STR24## H H 220-225 IP44 ##STR25## F H 263-274 A45 ##STR26## F F -- --46 ##STR27## F H >300 M______________________________________
REFERENCE EXAMPLE 47
Preparation of 4-(4-fluorophenyl)-1,5,6,7,8,9-hexahydro-2H-6,9-methanocyclohepta[b]pyridin-2-one
Bicyclo[3.2.1]octan-2-one (2 g) and 4-fluorobenzoylacetonitrile (2.6 g) are dissolved in 1,1,2,2-tetrachloroethane (5 ml) and thereto is added 75% polyphosphoric acid (25 g). The mixture is stirred at 80.degree. C. for 30 minutes, at 100.degree. C. for 1 hour, and further at 130.degree. C. for 30 minutes. After cooling, the reaction mixture is poured into ice-water and neutralized with potassium carbonate. The precipitated crystals are collected by filtration and washed successively with water and ethyl acetate. The resultant is recrystallized from methanol to give the desired product (2.7 g), m.p.>300.degree. C.
REFERENCE EXAMPLE 48
Preparation of 4-(4-fluorophenyl)-1,5,6,7,8,9-hexahydro-2H-5,8-methanocyclohepta[b]pyridin-2-one
In the same manner as described in Reference Example 47 except that the corresponding starting materials are used, there is obtained the desired compound.
REFERENCE EXAMPLE 49
Preparation of 2-chloro-4-(4-fluorophenyl)-5,6,7,8-tetrahydroquinoline:
A mixture of 4-(4-fluorophenyl)-5,6,7,8-tetrahydro-2(1H)-quinoline (18.7 g) and phenylphosphonic dichloride (29 ml) is stirred at 170.degree. C. for 1 hour. After cooling, the reaction mixture is dissolved in chloroform (200 ml) and the mixture is added dropwise into ice-water with stirring over a period of about 30 minutes. The mixture is made alkaline by dropwise addition of conc. aqueous ammonia thereto. The organic layer is separated, washed with water, dried over anhydrous sodium sulfate, and concentrated under reduced pressure. The residue is recrystallized from isopropanol-petroleum ether to give the desired product (15.1 g), m.p. 111.degree.-112.degree. C.
REFERENCE EXAMPLES 50 TO 94
In the same manner as described in Reference Example 49 except that the corresponding starting materials are used, there are obtained the compounds as shown in Table 10 and 11.
TABLE 10______________________________________ ##STR28##Ref. Melting point Solvent forEx. n R.sup.2 R.sup.3 (.degree.C.) recrystallization______________________________________50 3 H H 86-87 IP-PE51 3 4-F H 144-146 A52 3 3-F H 55-57 IP53 3 2-F H Oil --54 3 4-OMe H Oil --55 3 4-F 2-F Oil --56 4 H H 86-88 A57 4 3-F H 84-86 A58 4 2-F H 84-85 IP-PE59 4 4-OMe H 104-106 A60 5 H H 71-72 IP61 5 4-F H 84-85 PE62 5 3-F H Oil --63 5 2-F H 89-90 A64 5 4-OMe H 75-76 A65 5 4-Me H 66-67 A66 5 4-Cl H 117-118 MC-HX67 5 4-F 2-F 55-56 IP68 6 H H 96-97 IP-PE69 6 4-F H 136-137 A70 6 3-F H 110-111 A71 6 2-F H 81-82 IP72 6 4-F 3-F 137-138 A73 6 4-F 2-F 74-75 IP74 6 6-F 2-F 83-84 IP75 6 4-OMe H 147-150 MC-HX76 6 4-Me H 142-143 M77 6 4-Cl H 183-185 CF-T78 6 3-Cl H 100-101 A79 6 4-Br H Oil --80 7 4-F H 60-68 E______________________________________
TABLE 11______________________________________ ##STR29## Ex.Ref. ##STR30## R.sup.2 R.sup.3 (.degree.C.)pointMelting recrystallizationSolvent______________________________________ for81 ##STR31## H H 94-95 IP82 ##STR32## F H 94-95 A83 ##STR33## F H 74-75 IP84 ##STR34## F H 102-104 MC-HX85 ##STR35## H H 109-110 A86 ##STR36## F H 159-162 CF-A87 ##STR37## F H 83-84 IP88 ##STR38## F H -- --89 ##STR39## H H 63-65 IP-PE90 ##STR40## F H 74-76 IP91 ##STR41## F F Oil --92 ##STR42## F H 132-135 MC-HX93 ##STR43## H H Oil --94 ##STR44## F H 92-94 MC-HX______________________________________
EXAMPLE 1
Preparation of 2-(4-ethyl-1-piperazinyl)-4-(4-fluorophenyl)-5,6,7,8,9,10-hexahydrocycloocta[b]pyridine
A mixture of 2-chloro-4-(4-fluorophenyl)-5,6,7,8,9,10-hexahydrocycloocta[b]pyridine (2.0 g), N-ethylpiperazine (2.4 g), and potassium iodide (1.1 g) is stirred at 170.degree. C. for 5 hours. After cooling, the reaction mixture is dissolved in ethyl acetate and water. The organic layer is washed with water and extracted with 5% hydrochloric acid. The extract is made alkaline with potassium carbonate, and extracted with ethyl acetate. The extract is washed with water, dried over anhydrous sodium sulfate, and concentrated under reduced pressure.
(a) The residue is recrystallized from acetonitrile to give the desired product (1.2 g), m.p. 123.degree.-124.degree. C.
This product obtained in the above (a) is converted to the following salt thereof by treating the product with various acids.
(b) Dimaleate, m.p. 165.degree.-167.degree. C. (ethanol)
(c) Dihydrochloride.multidot.1/2 hydrate, m.p. 215.degree.-222.degree. C. (acetone)
(d) Fumarate, m.p. 228.degree.-230.degree. C. (ethanol)
(e) Citrate, m.p. 184.degree.-187.degree. C. (ethanol)
EXAMPLE 2
Preparation of 2-(4-ethyl-1-piperazinyl)-4-(2,4-difluorophenyl)-5,6,7,8,9,10-hexahydrocycloocta[b] pyridine
A mixture of 2-chloro-4-(2,4-difluorophenyl)-5,6,7,8,9,10-hexahydrocycloocta[b]pyridine (10 g), N-ethylpiperazine (11 g), and potassium iodide (5.4 g) is stirred at 170.degree. C. for 5 hours. After cooling, the reaction mixture is dissolved in chloroform and 5% aqueous potassium carbonate solution. The organic layer is washed with water, dried over anhydrous sodium sulfate, and concentrated under reduced pressure.
(a) The residue is dissolved in toluene, and subjected to silica gel column chromatography. The fractions eluted with toluene and a mixture of toluene-ethyl acetate (1:1) are collected, and recrystallized from isopropyl alcohol to give the desired product (5.5 g), m.p. 124.degree.-125.degree. C.
(b) The product obtained in the above (a) is treated with a solution of maleic acid in ethanol to give the dimaleate of the desired product, m.p. 133.degree.-135.degree. C. (ethanol).
EXAMPLE 3
Preparation of 2-(4-ethyl-1-piperazinyl)-4-phenyl-5,6,7,8-tetarahydroquinoline
A mixture of 2-chloro-4-phenyl-5,6,7,8-tetrahydroquinoline (1.0 g), N-ethylpiperazine (1.2 g), and potassium iodide (0.66 g) is stirred at 170.degree. C. for 15 hours. After cooling, the reaction mixture is dissolved in chloroform, and 5% aqueous potassium carbonate solution. The organic layer is washed with water, dried over anhydrous sodium sulfate, and concentrated under reduced pressure. The resulting oily residue is subjected to basic alumina column chromatography. The fractions eluted with toluene and a mixture of toluene and ethyl acetate (9:1) are collected, and treated with a solution of maleic acid in ethanol. The resulting maleate product is recrystallized from ethanolethyl acetate to give the dimaleate of the desired product (0.45 g), m.p. 139.degree.-142.degree. C.
EXAMPLES 4-77
In the same manner as described in Example 3 except that the corresponding starting materials are used, there are obtained the compounds as shown in Tables 12 and 13.
TABLE 12__________________________________________________________________________ ##STR45## M.p. Sol.Ex. n R.sup.2 R.sup.3 R.sup.1 Q (.degree.C.) recry.__________________________________________________________________________4 3 H H Et 3/2 Maleate 132-136 A5 3 H H CH.sub.2 CH.sub.2 OH Maleate 178-183 A6 3 4-F H Et Dimaleate 164-166 A7 3 4-F H CH.sub.2 CH.sub.2 OH 1/2 Fumarate 207-210 M8 3 3-F H Et Dimaleate 124-126 A9 3 3-F H CH.sub.2 CH.sub.2 OH Maleate.1/4 H.sub.2 O 182-185 A10 3 2-F H Et Maleate.1/5 H.sub.2 O 189-191 A11 3 2-F H CH.sub.2 CH.sub.2 OH Maleate.1/2 H.sub.2 O 186-188 A12 3 4-OMe H Et Dimaleate 174-176 A13 3 4-OMe H CH.sub.2 CH.sub.2 OH Dioxalate 183-187 M14 4 H H CH.sub.2 CH.sub.2 OH Dimaleate 129-131 A15 4 4-F H Et Dimaleate 146-148 A16 4 4-F H CH.sub.2 CH.sub.2 OH Dimaleate 149-151 A17 4 3-F H Et Dimaleate 133-136 M18 4 3-F H CH.sub.2 CH.sub.2 OH Dioxalate 180-182 M19 4 2-F H Et Dimaleate 148-150 A20 4 2-F H CH.sub.2 CH.sub.2 OH Dioxalate.1/4 H.sub.2 O 169-172 A21 4 4-OMe H Et Dimaleate 179-181 A22 4 4-OMe H CH.sub.2 CH.sub.2 OH Dioxalate.1/4 H.sub.2 O 160-165 A23 5 H H Et Dimaleate 150-152 IP24 5 H H CH.sub.2 CH.sub.2 OH 1/2 Fumarate 142-144 A25 5 4-F H Et Dimaleate 150-152 A26 5 4-F H CH.sub.2 CH.sub.2 OH 1.7 Oxalate 180-182 A27 5 3-F H Et Dimaleate 138-140 A28 5 3-F H CH.sub.2 CH.sub.2 OH Maleate.1/4 H.sub.2 O 155-158 IP29 5 2-F H Et Oxalate 231-234 A-M30 5 2-F H CH.sub.2 CH.sub.2 OH 1/2 Fumarate.1/4 H.sub.2 O 163-164 M31 5 4-Cl H Et -- 150-151 MC-A32 5 4-OMe H Et Dimaleate 162-164 A33 5 4-OMe H CH.sub.2 CH.sub.2 OH Fumarate.1/2 H.sub.2 O 190-195 A-M34 5 4-Me H Et Dimaleate 180-183 A35 5 4-Me H CH.sub.2 CH.sub.2 OH Dimaleate.1/4 H.sub.2 O 151-153 IP36 6 H H Et Fumarate 236-238 M37 6 4-F H CH.sub.2 CH.sub.2 OH Dimaleate 135-137 A38 6 4-F H ##STR46## -- 167-168 A-AT39 6 4-F H ##STR47## -- 165-167 M40 6 4-F H ##STR48## -- 156-157 A-AT41 6 4-F H ##STR49## -- 206-208 M-AT42 6 3-F H Et Dimaleate 153-155 A43 6 2-F H Et Fumarate 201-204 A44 6 4-Cl H Et -- 133-135 CF-IP45 6 4-Cl H CH.sub.2 CH.sub.2 OH -- 156-158 CF-A46 6 3-Cl H Et -- 114-115 IP47 6 3-Cl H CH.sub.2 CH.sub.2 OH Oxalate 210-212 M48 6 4-Br H Et -- 134-136 A49 6 4-OMe H Et -- 113-114 MC-HX50 6 4-OMe H CH.sub.2 CH.sub.2 OH HCl.3/4 H.sub.2 O 153-156 A-E51 6 4-Me H Et Dimaleate 163-164 A52 6 4-Me H CH.sub.2 CH.sub.2 OH Oxalate 207-209 A53 6 4-F 2-F CH.sub.2 CH.sub.2 OH Dioxalate.1/10 H.sub.2 O 169-172 A54 6 4-F 3-F Et -- 105-106 AC55 6 6-F 2-F Et -- 104-105 AC56 7 4-F H Et Fumarate 184-189 A-E__________________________________________________________________________
TABLE 13__________________________________________________________________________ ##STR50## Ex. ##STR51## R.sup.2 R.sup.1 Q M.p. (.degree.C.) Sol. recry.__________________________________________________________________________57 ##STR52## H Et Dimaleate 138-140 A58 ##STR53## H CH.sub.2 CH.sub.2 OH Dimaleate 140-141 A59 ##STR54## F Et Dioxalate.1/4 H.sub.2 O 126-127 A60 ##STR55## F CH.sub.2 CH.sub.2 OH Fumarate 160-161 A61 ##STR56## H Et -- 130-131 A62 ##STR57## H CH.sub.2 CH.sub.2 OH -- 162-163 A63 ##STR58## F Et Fumarate 212-215 M-A64 ##STR59## F Et Dimaleate 141-142 IP65 ##STR60## F CH.sub.2 CH.sub.2 OH Dimaleate.1/2 H.sub.2 O 120-122 A66 ##STR61## F Et Fumarate 209-211 M-A67 ##STR62## F CH.sub.2 CH.sub.2 OH HCl 200-203 A-E68 ##STR63## F Et -- 107-108 IP69 ##STR64## F CH.sub.2 CH.sub.2 OH Dioxalate.1/4 H.sub.2 O 125-126 A70 ##STR65## F Et Fumarate 203-204 A71 ##STR66## H Et Dimaleate.1/4 H.sub.2 O 173-176 A72 ##STR67## H CH.sub.2 CH.sub.2 OH Dimaleate 140-142 A73 ##STR68## F Et Dimaleate 163-169 IP74 ##STR69## F CH.sub.2 CH.sub.2 OH Dimaleate 159-161 A75 ##STR70## F Et Fumarate 234-236 M-A76 ##STR71## F Et Fumarate 216-219 M-A77 ##STR72## F Et Fumarate.1/4 H.sub.2 O 226-230 M-A__________________________________________________________________________
EXAMPLES 78-85
In the same manner as described in Example 3 except that the corresponding starting materials are used, there are obtained the following compounds.
(Example 78)
2-(4-Methyl-1-homopiperazinyl)-4-(4-fluorophenyl)-6,7,8,9-tetrahydro-5H-cyclohepta[b]pyridine maleate, m.p. 187.degree.-189.degree. C. (ethanol)
(Example 79)
2-(4-Butanoyl-1-homopiperazinyl)-4-(4-fluorophenyl)-6,7,8,9-tetrahydro-5H-cyclohepta[b]pyridine, oily product
(Example 80)
2-(4-Methyl-1-homopiperazinyl)-4-(4-fluorophenyl)-5,6,7,8,9,10-hexahydrocycloocta[b]pyridine dimaleate, m.p. 141.degree.-143.degree. C. (ethanol)
(Example 81)
2-(4-Benzoyl-1-homopiperazinyl)-4-(4-fluorophenyl)-5,6,7,8,9,10-hexahydrocycloocta[b]pyridine, oily product
(Example 82)
2-[4-(2-Methoxyethyl)-1-piperazinyl]-4-(4-fluorophenyl)-5,6,7,8,9,10-hexahydrocycloocta[b]pyridine dimaleate, m.p. 119.degree.-120.degree. C. (acetonitrile)
(Example 83)
2-(3-Methyl-1-piperazinyl)-4-(4-fluorophenyl)-5,6,7,8,9,10-hexahydrocycloocta[b]pyridine, m.p. 138.degree.-141.degree. C. (isopropyl alcohol-hexane)
(Example 84)
2-(3,5-Dimethyl-1-piperazinyl)-4-(4-fluorophenyl)-5,6,7,8,9,10-hexahydrocycloocta[b]pyridine 1/2 fumarate, m.p. 262.degree.-266.degree. C. (decomposed) (methanol)
(Example 85)
2-(1-Homopiperazinyl)-4-(4-fluorophenyl)-5,6,7,8,9,10-hexahydrocycloocta[b]pyridine maleate, m.p. 198.degree.-200.degree. C. (ethanol)
EXAMPLE 86
Preparation of 2-(1-piperazinyl)-4-phenyl-5,6,7,8-tetrahydroquinoline
A mixture of 2-(4-benzyl-1-piperazinyl)-4-phenyl-5,6,7,8-tetrahydroquinoline (m.p. 105.degree.-107.degree. C., 2.1 g) obtained in the same manner as in Example 3, chloroethyl chloroformate (0.86 g) and methylene chloride (40 ml) is refluxed for 1 hour. The reaction mixture is concentrated under reduced pressure, and methanol (40 ml) is added to the residue. The mixture is refluxed for 30 minutes and concentrated under reduced pressure. The resulting residue is dissolved in water, washed with diethyl ether, neutralized with potassium carbonate, and extracted with chloroform. The extract is washed with water, dried over anhydrous sodium sulfate, and the solvent is distilled off under reduced pressure. To the residue is added a solution of maleic acid in ethanol. The resulting maleate product is recrystallized from ethanol to give the dimaleate of the desired product (1.2 g), m.p. 150.degree.-151.degree. C.
EXAMPLES 87-110
In the same manner as described in Example 86 except that the corresponding starting materials are used, there are obtained the compounds as shown in Tables 14 and 15.
TABLE 14______________________________________ ##STR73## Melting Solvent point for re-Ex. n R.sup.2 R.sup.3 Q (.degree.C.) crystaln.______________________________________87 3 H H Maleate 175-179 A88 3 4-F H Dimaleate 152-155 A89 3 3-F H Maleate 176-179 A90 3 2-F H Maleate 186-188 A91 3 4-OMe H Dimaleate 148-149 A92 4 4-F H Dimaleate.1/4 H.sub.2 O 143-145 A93 4 3-F H Dimaleate.1/4 H.sub.2 O 148-151 A94 4 2-F H Dimaleate 136-137 A95 4 4-OMe H Dioxalate.1/4 H.sub.2 O 210-213 M96 5 H H Maleate 173-176 A97 5 4-F H Dimaleate 144-147 A98 5 3-F H Maleate 173-175 A99 5 2-F H Maleate 173-180 A100 5 4-Cl H Maleate.1/4 H.sub.2 O 176-178 M-A101 5 4-OMe H Dimaleate 133-137 IP102 5 4-Me H Dimaleate.3/4 H.sub.2 O 95-97 IP103 6 H H Dimaleate.1/4 H.sub.2 O 124-127 A104 6 4-F H Maleate 186-190 A105 6 4-Me H Fumarate 218-221 A106 6 4-F 2-F 1/2 Fumarate.1/2 H.sub.2 O 177-179 A______________________________________
TABLE 15__________________________________________________________________________ ##STR74## Ex. ##STR75## R.sup.2 Q (.degree.C.)Melting point recrystaln.Solvent for__________________________________________________________________________107 ##STR76## H Dimaleate.1/4 H.sub.2 O 147-149 A108 ##STR77## H 3/2 Oxalate.1/2 H.sub.2 O 178-181 M109 ##STR78## F 1/2 Fumarate.1/10 H.sub.2 O 181-186 A110 ##STR79## H Maleate 207-208 A__________________________________________________________________________
EXAMPLE 111
Preparation of 2-(4-methyl-1-piperazinyl)-4-(4-fluorophenyl)-6,7-dihydro-5H-1-pyrindine
A mixture of 2-(1-piperazinyl)-4-(4-fluorophenyl)-6,7-dihydro-5H-1-pyrindine (2 g), 37% aqueous formaldehyde solution (0.66 g), formic acid (0.68 g) and water (15 ml) is refluxed for 20 minutes. After cooling, the reaction mixture is made alkaline with diluted aqueous sodium hydroxide solution, and extracted with ethyl acetate. The extract is washed with water, dried over anhydrous sodium sulfate, and concentrated under reduced pressure. To the residue is added a solution of maleic acid in ethanol, and the resulting maleate product is recrystallized from ethanol to give the maleate of the desired compound, m.p. 135.degree.-137.degree. C.
EXAMPLES 112-117
In the same manner as described in Example 111 except that the corresponding starting materials are used, there are obtained the following compounds.
(Example 112)
2-(4-Methyl-1-piperazinyl)-4-(4-fluorophenyl)-5,6,7,8,9,10-hexahydrocycloocta[b]pyridine dimaleate, m.p. 136.degree.-138.degree. C. (ethanol)
(Example 113)
2-(4-Methyl-1-piperazinyl)-4-(4-methylphenyl)-5,6,7,8,9,10-hexahydrocycloocta[b]pyridine dimaleate, m.p. 152.degree.-154.degree. C. (ethanol)
(Example 114)
2-(4-Methyl-1-piperazinyl)-4-(2,4-difluorophenyl)-5,6,7,8,9,10-hexahydrocycloocta[b]pyridine, m.p. 132.degree.-133.degree. C. (ethanol)
(Example 115)
2-(4-Methyl-1-piperazinyl)-4-(4-fluorophenyl)-6-methyl-5,6,7,8-tetrahydroquinoline dimaleate, m.p. 161.degree.-164.degree. C. (ethanol)
(Example 116)
2-(3,4-Dimethyl-1-piperazinyl)-4-(4-fluorophenyl)-5,6,7,8,9,10-hexahydrocycloocta[b]pyridine fumarate 1/4 hydrate, m.p. 173.degree.-175.degree. C. (ethanol-diethyl ether)
(Example 117)
2-(cis-3,5-Dimethyl-4-methyl-1-piperazinyl)-4-(4-fluorophenyl)-5,6,7,8,9,10-hexahydrocycloocta[b]pyridine fumarate, m.p. 208.degree.-210.degree. C. (methanol-ethanol)
EXAMPLE 118
Preparation of 2-(4-n-propyl-1-piperazinyl)-4-(4-fluorophenyl)-5,6,7,8,9,10-hexahydrocycloocta[b]pyridine
A mixture of 2-(1-piperazinyl)-4-(4-fluorophenyl)-5,6,7,8,9,10-hexahydrocycloocta[b]pyridine (1.4 g), n-propyl bromide (0.56 g), potassium carbonate (0.68 g), potassium iodide (0.1 g) and methanol (50 ml) is refluxed for 15 hours. The reaction mixture is concentrated under reduced pressure and thereto is added water. The mixture is extracted with ethyl acetate, washed with water, dried over anhydrous sodium sulfate, and the solvent is distilled off under reduced pressure. To the residue is added a solution of maleic acid in ethanol, and the resulting maleate product is recrystallized from ethanol to give the dimaleate of the desired compound (0.6 g), m.p. 149.degree.-152.degree. C.
EXAMPLES 119-126
In the same manner as described in Example 118 except that the corresponding starting materials are used, there are obtained the compounds as shown in the following Table 16.
TABLE 16______________________________________ ##STR80## M.p. Solvent, forEx. n R.sup.1 Q (.degree.C.) recrystal.______________________________________119 3 (CH.sub.2).sub.3 OH Maleate 174-178 A120 3 ##STR81## Maleate 215-218 A121 6 ##STR82## Dimaleate 154-155 A122 6 (CH.sub.2).sub.3 CH.sub.3 -- 89-90 A123 6 (CH.sub.2).sub.4 CH.sub.3 Maleate 213-217 A-M124 6 (CH.sub.2).sub.5 CH.sub.3 Maleate 196-197 A125 6 CH.sub.2 CHCH.sub.2 1/4 H.sub.2 O 109-110 AC126 6 CH.sub.2 C CH -- 75-77 HX______________________________________
EXAMPLE 127
Preparation of 2-(4-n-propyl-1-piperazinyl)-4-(2,4-difluorophenyl)-5,6,7,8,9,10-hexahydrocycloocta[b]pyridine
In the same manner as described in Example 118 except that the corresponding starting materials are used, the desired product is obtained, m.p. 108.degree.-109.degree. C. (ethanol).
EXAMPLE 128
Preparation of 2-[4-(2-furoyl)-1-piperazinyl]-4-phenyl-5,6,7,8-tetrahydroquinoline
A mixture of 2-(1-piperazinyl)-4-phenyl-5,6,7,8-tetrahydroquinoline (1.2 g), 2-furancarboxylic acid (0.46 g), chloroform (40 ml) and 1-(3-dimethylaminopropyl) 3-ethylcarbodiimide hydrochloride (0.79 g) is stirred at 25.degree. C. for 2 hours. The reaction mixture is washed with water, dried over anhydrous sodium sulfate, and the solvent is distilled off under reduced pressure. The residue is dissolved in toluene and subjected to silica gel column chromatography. The fractions eluted with toluene and toluene-ethyl acetate (9:1) are collected and recrystallized from ethanol to give the desired product (0.4 g), m.p. 128.degree.-130.degree. C.
EXAMPLE 129
Preparation of 2-[4-(2-furoyl)-1-piperazinyl]-4-(4-fluorophenyl)-6,7-dihydro-5H-1-pyrindine
In the same manner as described in Example 128 except that the corresponding starting materials are used, the desired product is obtained, m.p. 165.degree.-166.degree. C. (ethanol).
EXAMPLE 130
Preparation of 2-(4-ethyl-1-piperazinyl)-4-(4-hydroxyphenyl)-5,6,7,8,9,10-hexahydrocycloocta[b]pyridine
2-(4-Ethyl-1-piperazinyl)-4-(4-methoxyphenyl)-5,6,7,8,9,10-hexahydrocycloocta[b]pyridine (1.3 g) is dissolved in 48% hydrobromic acid (10 ml) and the mixture is stirred at 120.degree. C. for 2 hours. After cooling, water is added thereto and the mixture is neutralized with 1N aqueous sodium hydroxide solution and sodium hydrogen carbonate. The resulting precipitate is collected by filtration, washed with water, and recrystallized from methanol to give the desired product (0.6 g), m.p. 250.degree.-253.degree. C.
EXAMPLE 131
Preparation of 2-(4-ethyl-1-piperazinyl)-4-(2,4-difluorophenyl)-6,7,8,9-tetrahydro-5H-cyclohepta[b]pyridine
In the same manner as described in Example 3 except that the corresponding starting materials are used, there is obtained the oxalate .multidot.1/2 hydrate of the desired product, m.p. 225.degree.-227.degree. C. (methanol).
EXAMPLE 132
Preparation of 2-(4-ethyl-1-piperazinyl)-4-(2,4-difluorophenyl)-6,7-dihydro-5H-1-pyrindin
In the same manner as described in Example 3 except that the corresponding starting materials are used, there is obtained the maleate of the desired product, m.p. 195.degree.-196.degree. C. (ethanol).
EXAMPLE 133
Preparation of 2-(4-cyclopropyl-1-piperazinyl)-4-(4-fluorophenyl)-5,6,7,8,9,10-hexahydrocycloocta[b]pyridine
In the same manner as described in Example 3 except that the corresponding starting materials are used, there is obtained the desired product, m.p. 125.degree.-128.degree. C. (ethanol).
EXAMPLE 134
Preparation of 2-[4-(4-fluorophenyl)-1-piperazinyl]-4-(4-fluorophenyl)-5,6,7,8,9,10-hexahydrocycloocta[b]pyridine
In the same manner as described in Example 3 except that the corresponding starting materials are used, there is obtained the desired product, m.p. 130.degree.-131.degree. C. (methanol).
EXAMPLE 135
Preparation of 2-(4-ethyl-1-piperazinyl)-4-(2,4-difluorophenyl)-5,6,7,8-tetrahydro-5,8-methanoquinoline
In the same manner as described in Example 3 except that the corresponding starting materials are used, there is obtained the oxalate .multidot.1/2 hydrate of the desired product, m.p. 234.degree.-238.degree. C. (ethanol).
EXAMPLE 136
Preparation of 2-(cis-3,5-dimethyl-4-methyl-1-piperazinyl)-4-(4-fluorophenyl)-6,7-dihydro-5H-1-pyrindine
In the same manner as described in Example 111 except that the corresponding starting materials are used, there is obtained the fumarate .multidot.1/4 hydrate of the desired product, m.p. 204.degree.-208.degree. C. (methanol-ethanol).
EXAMPLE 137
Preparation of 2-{4-[3-(4-fluorobenzoyl)propyl]-1-piperazinyl}-4-(4-fluorophenyl)-6,7-dihydro-5H-1-pyrindine
In the same manner as described in Example 118 except that the corresponding starting materials are used, there is obtained the desired product, m.p. 115.degree.-116.degree. C. (ethanol).
EXAMPLE 138
Preparation of 2-[4-(3-hydroxypropyl)-1-piperazinyl]-4-(4-fluorophenyl)-5,6,7,8,9,10-hexahydrocycloocta[b]pyridine
In the same manner as described in Example 118 except that the corresponding starting materials are used, there is obtained the dioxalate .multidot.1/4 hydrate of the desired product, m.p. 136.degree.-138.degree. C. (methanol).
EXAMPLE 139
Preparation of 2-[4-(2-acetoxyethyl)-1-piperazinyl]-4-(4-fluorophenyl)-5,6,7,8,9,10-hexahydrocycloocta[b]pyridine
A mixture of 2-[4-(2-hydroxyethyl)-1-piperazinyl]-4-(4-fluorophenyl)-5,6,7,8,9,10-hexahydrocycloocta[b]pyridine (1.5 g), acetic anhydride (0.6 g), triethylamine (0.7 g) and ethyl acetate (30 ml) is refluxed for 2 hours. After cooling, the reaction mixture is washed with water, dried over anhydrous sodium sulfate, and concentrated under reduced pressure. The residue is dissolved in toluene and the mixture is subjected to silica gel column chromatography. The fractions eluted with toluene and toluene-ethyl acetate (1:1) are collected and thereto is added a solution of maleic acid in ethanol. The resulting maleate product is recrystallized from ethanol to give the maleate of the desired product (0.65 g), m.p. 187.degree.-191.degree. C.
EXAMPLE 140
Preparation of 2-(4-cyclopropylmethyl-1-piperazinyl)-4-(4-fluorophenyl)-5,6,7,8,9,10-hexahydrocycloocta[b]pyridine
In the same manner as described in Example 118 except that the corresponding starting materials are used, there is obtained the desired product, m.p. 109.degree.-110.degree. C. (methylene chloride-hexane).
The preparation of the pharmaceutical composition of the present invention is illustrated by the following Preparations.
______________________________________Preparation 1Preparation of capsules:Components Amount______________________________________2-(4-Ethyl-1-piperazinyl)-4-(4-fluorophenyl)- 5 g6,7,8,9-tetrahydro-5H-cyclohepta[b]pyridinedimaleateCorn starch 57 gLactose 10 gCrystalline cellulose 25 gHydroxypropyl cellulose 2 gLight silicic anhydride 0.5 gMagnesium stearate 0.5 g______________________________________
According to a conventional method, the above components are mixed and granulated, and the granules thus obtained are packed in capsules (1000 capsules) to give capsules containing the granules of 100 mg per one capsule.
______________________________________Preparation 2Preparation of tablets:Components Amount______________________________________2-(4-Ethyl-1-piperazinyl)-4-(4-fluorophenyl)- 5 g5,6,7,8,9,10-hexahydrocycloocta[b]pyridinedimaleateCorn starch 20 gLactose 30 gCrystalline cellulose 30 gHydroxypropyl cellulose 5 gLow sustituted hydroxypropyl cellulose 10 g______________________________________
According to a conventional method, the above components are mixed and granulated, and the granules thus obtained are mixed with light silicic anhydride and magnesium stearate, and the mixture is tabletted to give tablets containing the active ingredient of 5 mg per one tablet.
______________________________________Preparation 3Preparation of powders:Components Amount______________________________________2-(4-Ethyl-1-piperazinyl)-4-(4-fluorophenyl)- 5 g5,6,7,8,9,10-hexahydrocycloocta[b]pyridinedimaleateCorn starch 173 gLactose 300 gHydroxypropyl cellulose 20 g______________________________________
According to a conventional method, the above components are mixed, granulated and screened, and the granules thus obtained are mixed with an appropriate amount of light silicic anhydride to give powders (100 triturations).
______________________________________Preparation 4Preparation of injections:Components Amount______________________________________2-(4-Ethyl-1-piperazinyl)-4-(4-fluorophenyl)- 5 g5,6,7,8,9,10-hexahydrocycloocta[b]pyridinedimaleateD-Sorbitol 45 g1N Aqueous solution of maleic acid q.s.or sodium hydroxideDistilled water for injection q.s.Totally 1000 ml______________________________________
The above active ingredient and D-sorbitol are mixed with distilled water for injection, and thereto is added 1N aqueous solution of maletic acid or sodium hydroxide to adjust the solution to pH 4.0. The solution is filterd with a membrane filter (pore size, 0.22 .mu.m) and packed in ampoule (content 10 ml). The ampoule is sealed by melting and sterilized with high pressure steam at 121.degree. C. for 20 minutes to give injection solutions.
______________________________________Preparation 5Preparation of lyophilized preparation:Components Amount______________________________________2-(4-Ethyl-1-piperazinyl)-4-(4-fluorophenyl)- 5 g5,6,7,8,9,10-hexahydrocycloocta[b]pyridinedimaleateD-Mannitol 45 g1N Aqueous solution of maleic acid q.s.of sodium hydroxideDistilled water for injection q.s.Totally 1000 ml______________________________________
The above active ingredient and D-mannitol are mixed with distilled water for injection, and thereto is added 1N aqueous solution of maleic acid or sodium hydroxide to adjust the solution to pH 4.0. The solution is filtered with a membrane filter (pore size, 0.22 .mu.m) and packed in a vial (content 10 ml). The vial is sealed with a rubber stopper in halfway and subjected to lyophilization, that is, pre-freezing, primary drying at -50.degree. C., secondary drying at -20.degree. C., and then final drying at 20.degree. C. After completely sealed with a rubber stopper within a chamber, the vial is covered with a flip-off cap to give a lyophilized preparation.

Number	Name	Date	Kind
4469696	Rosentreter et al.	Sep 1984
4831034	Barreau et al.	May 1989

2-(1-Piperazinyl)-4-phenylcycloalkanopyridine derivatives, processes for the production thereof, and pharmaceutical composition containing the same

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