Patent Application
20170321241
Generally, the present invention relates to the field of steroid hydroxylation. More specifically, the present invention relates to a method for the 21-hydroxylation of steroids in cells. It also relates to cells expressing a steroid 21-hydroxylating enzyme or steroid 21-hydroxylase, expression vectors comprising a nucleic acid encoding for a steroid 21-hydroxylase and a kit for carrying out the method for the 21-hydroxylation of steroids in cells.
Synthetic glucocorticoids are descendent from the natural occurring stress hormone cortisol and play a crucial role in pharmaceutical industry because of their anti-inflammatory and immune suppressive effects. Moreover, synthetic molecules often act more effective than cortisol.
Currently, the synthesis of some pharmaceutically active steroids involves a 21-hydroxylation of their precursor (see
This problem has been addressed by the present inventors by the development of whole cell biotransformation of steroids in a one-step synthesis by the enzyme CYP21A2, which is a member of the protein family of the cytochrome P450 monooxygenases and which is able to perform a highly selective hydroxylation of steroids at the 21-position of the steroid scaffold (see
Before the present invention is described in detail below, it is to be understood that this invention is not limited to the particular methodology, protocols and reagents described herein as these may vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments only, and is not intended to limit the scope of the present invention which will be limited only by the appended claims. Unless defined otherwise, all technical and scientific terms used herein have the same meanings as commonly understood by one of ordinary skill in the art.
Preferably, the terms used herein are defined as described in “A multilingual glossary of biotechnological terms: (IUPAC Recommendations)”, Leuenberger, H. G. W, Nagel, B. and Kölbl, H. eds. (1995), Helvetica Chimica Acta, CH-4010 Basel, Switzerland).
Several documents are cited throughout the text of this specification. Each of the documents cited herein (including all patents, patent applications, scientific publications, manufacturer's specifications, instructions etc.), whether supra or infra, is hereby incorporated by reference in its entirety. Nothing herein is to be construed as an admission that the invention is not entitled to antedate such disclosure by virtue of prior invention.
In the following, the elements of the present invention will be described. These elements are listed with specific embodiments, however, it should be understood that they may be combined in any manner and in any number to create additional embodiments. The variously described examples and preferred embodiments should not be construed to limit the present invention to only the explicitly described embodiments. This description should be understood to support and encompass embodiments which combine the explicitly described embodiments with any number of the disclosed and/or preferred elements. Furthermore, any permutations and combinations of all described elements in this application should be considered disclosed by the description of the present application unless the context indicates otherwise.
Throughout this specification and the claims which follow, unless the context requires otherwise, the word “comprise”, and variations such as “comprises” and “comprising”, are to be understood to imply the inclusion of a stated integer or step or group of integers or steps but not the exclusion of any other integer or step or group of integer or step. As used in this specification and the appended claims, the singular forms “a”, “an”, and “the” include plural referents, unless the content clearly dictates otherwise.
In a first aspect, the present invention relates to a process for the hydroxylation of the carbon atom 21 of a steroid, comprising the steps of:
a) providing a cell expressing
A steroid is a type of organic compound that contains a characteristic arrangement of four cycloalkane rings that are joined to each other (shown below). The core of steroids is composed of seventeen carbon atoms bonded together that take the form of four fused rings: three cyclohexane rings (designated as rings A, B and C) and one cyclopentane ring (the D ring). The steroids vary by the functional groups attached to this four-ring core and by the oxidation state of the rings.
The hydroxylation of the carbon atom 21 of a steroid is the addition of an —OH group at position 21 as shown in the above-shown ABCD steroid ring system. The numbering of the carbon atoms is according to the IUPAC (International Union of Pure and Applied Chemistry)-approved ring lettering and atom numbering. The 21-hydroxylation of a steroid is shown in
In a particular embodiment of the process of the first aspect of the invention, the steroid is a 3-keto steroid. More particularly, the steroid is a non-natural steroid, i.e. a steroid that is not produced and/or 21-hydroxylated in cells, especially human or bovine cells, which are not genetically altered.
In one embodiment, the steroid is selected from the group consisting of medrane, deltamedrane, progesterone, 17OH-progesterone, medroxyprogesterone, and 5-α-dihydro-progesterone. The 21-hydroxylation converts these particular steroids to premedrol, medrol, 11-deoxycorticosterone, 11-deoxycortisol, 21OH-medroxyprogesterone and 21OH-(5α-dihydroprogesterone), respectively. In one particular embodiment, in which the steroid is a non-natural steroid, the steroid is selected from the group consisting of medrane, deltamedrane, medroxyprogesterone, and 5-α-dihydro-progesterone.
The cell is in particular a cultured cell, cultured in any cell medium, e.g. in a growth medium, and is, in a particular embodiment, in a resting state. According to this embodiment, the cell is comprised in a buffer or medium capable of maintaining the cell rather than in a growth medium. The composition of the buffer depends on the particular cell and suitable buffers are well-known in the art. Depending on the cell-type, the cell is a suspension cell or an adherent cell. A suspension cell is a cell that may naturally live in suspension (i.e. without being attached to a surface), or a cell that has been modified to be able to survive in suspension cultures, for example to be grown to higher densities than adherent conditions would allow. An adherent cell is a cell that requires a surface, such as tissue culture plastic or microcarrier, which may be coated with extracellular matrix (such as collagen and laminin) components to increase adhesion properties and provide other signals needed for growth and differentiation. In one embodiment, the adherent cell is a monolayer cell.
Generally, the cell may be a prokaryotic cell or a eukaryotic cell. A particular example of a prokarytic cell to be used in the process of the first aspect is an E. coli cell, e.g. of the E. coli strain C43(DE3) of the examples. A particular example of a eukaryotic cell is a yeast cell, e.g. a S. cerevisae cell or a Schizosaccharomyces pombe cell. However, the process of the first aspect is not limited to any particular cell type and any cell type may be used, in particular any cell type that can be grown and maintained in culture and that can be used as a recombinant expression system, like insect cells or mammalian cells in addition to the above-mentioned cells.
The term “heterologous” means that a protein is expressed in cell that does not normally (i.e. without human intervention) express that protein. CYP21A2, for example is an enzyme also called 21-hydroxylase, which is part of the cytochrome P450 family of enzymes. Cytochrome P450 enzymes are involved in many processes in the body, such as assisting with reactions that break down drugs and helping to produce cholesterol, certain hormones, and fats (lipids). The 21-hydroxylase enzyme is found in the adrenal glands, which are located on top of the kidneys and produce a variety of hormones that regulate many essential functions in the body. Therefore, with respect to heterologous expression, the CYP21A2 protein can be considered heterologous regarding any cell which is not an adrenal gland cell.
In particular, the term “heterologous” can also refer to the species a protein or gene is derived from in comparison to the cell in which it is expressed, in particular recombinantly expressed. A heterologous protein is then a protein that is derived from a different species than the cell it is expressed in, i.e. the cell of the process of the first aspect of the invention.
For example, the present inventors expressed mammalian, in particular human or bovine proteins in an E. coli cell, making these proteins heterologous with respect to the cell.
In a particular embodiment of the process of the first aspect of the invention, the CYP21A2 protein is of human or bovine origin. Human CYP21A2 (UniProt accession number P08686) has the sequence according to SEQ ID NO: 1 of the sequence listing. Bovine CYP21A2 (UniProt accession number P00191) has the sequence according to SEQ ID NO: 2 of the sequence listing. See also
The term “functional variant” is a protein variant that has at least 20% (e.g., at least: 20%; 30%; 40%; 50%; 60%; 70%; 80%; 90%; 95%; 98%; 99%; 99.5%; or 100% or even more) of the ability of the unaltered or CYP21A2 protein to 21-hydroxylate a steroid. This ability can be determined by the skilled person without undue burden using, for example, the methods shown in Examples 1 and 2 herein.
A “protein variant” is a protein that has an amino acid sequence that it at least 70%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% identical to the amino acid sequence of the CYP21A2 it is derived from, for example SEQ ID NO: 1 or 3 in case of human CYP21A2 or SEQ ID NO: 2 or 4 in case of bovine CYP21A2. The determination of percent identity between two sequences is accomplished using the mathematical algorithm of Karlin and Altschul, Proc. Natl. Acad. Sci. USA 90, 5873-5877, 1993. Such an algorithm is incorporated into the BLASTN and BLASTP programs of Altschul et al. (1990) J. Mol. Biol. 215, 403-410. To obtain gapped alignments for comparative purposes, Gapped BLAST is utilized as described in Altschul et al. (1997) Nucleic Acids Res. 25, 3389-3402. When utilizing BLAST and Gapped BLAST programs, the default parameters of the respective programs are used.
Alternatively, a protein variant can also be defined as having up to 100, 90, 80, 70, 60, 50, 40, 30, 20, 10, 5, 4, 3, 2, or 1 amino acid substitutions, in particular conservative amino acid substitutions. Conservative substitution tables are well known in the art (see for example Creighton (1984) Proteins. W. H. Freeman and Company). An overview of physical and chemical properties of amino acids is given in Table 1 below. In a particular embodiment, conservative substitutions are substitution made with amino acids have the same properties according to Table 1.
The term “variant” also includes protein fragments. A fragment of CYP21A2 has an N-terminal and/or C-terminal deletion of up to 100, 90, 80, 70, 60, 50, 40, 30, 20, 10, 5, 4, 3, 2, or 1 amino acids in total. In a particular embodiment, the functional variant is a fragment of CYP21A2 lacking the hydrophobic anchor region, i.e. a truncation of 29 N-terminal amino acid residues (
In addition, the CYP21A2 protein may be modified, for example by N-terminal or C-terminal amino acid additions, such as tags or N-terminal modifications for improved bacterial expression. For example, the tag may be a C-terminal His-tag, e.g. 6× His-tag and the N-terminal modification an addition of ten amino acids from the N-terminus of CYP2C3 (see
An electron transfer system is a series of compounds that transfer electrons from electron donors to electron acceptors via redox reactions. The term “capable of transferring electrons to CYP21A2” thereby means that CYP21A2 is the electron acceptor of this series. The term “series” herein may include CYP21A2 and, thus, the electron transfer system may consist of only one protein in addition to CYP21A2. For the process of the first aspect of the invention, it is not crucial which members the electron transfer system consists of, as long as the system is capable of transferring electrons to CYP21A2. Suitable systems are well-known in the art. In a particular embodiment of the process of the first aspect of the invention, the at least one electron transfer system comprises (i) a CYP21A2 reductase, or (ii) a ferredoxin reductase, preferably an NADPH-dependent ferredoxin reductase (adrenodoxin reductase) and a ferredoxin. For example, the at least one electron transfer protein can be selected from the group consisting of an NADPH-dependent cytochrome P450 reductase (CPR, e.g. human or bovine), the combination of an adrenodoxin reductase (AdR, e.g. human or bovine) and an adrenodoxin (Adx4-108, e.g. human or bovine), the combination of a flavodoxin reductase (Fpr, e.g. from E. coli) and an adrenodoxin (Adx4-108, e.g. human or bovine), the combination of an adrenodoxin reductase homolog (Arh), e.g. S. pombe adrenodoxin reductase homolog (Arh1), and an adrenodoxin (Adx4-108, e.g. human or bovine), the combination of an adrenodoxin reductase homolog (Arh), e.g. S. pombe adrenodoxin reductase homolog (Arh1), and the ferredoxin domain of an electron transfer domain (etpfd), e.g. the ferredoxin domain of the S. pombe electron transfer domain (etp1fd), and the combination of a flavodoxin reductase (Fpr, e.g. from E. coli) and the ferredoxin domain of an electron transfer domain (etpfd), e.g. the ferredoxin domain of the S. pombe electron transfer domain (etp1fd).
In a particular embodiment, exogenous NADPH is not added to the cell. In this embodiment, NADPH is produced by the cell. In a specific embodiment, the NADPH-production in the cell may be increased by recombinant means, e.g. the heterologous expression of enzymes involved in NADPH production (e.g. one or more of glucose-6-phosphate dehydrogenase (G6PD), phosphogluconate dehydrogenase (PGD), malate dehydrogenase (MDH), and/or isocitrate dehydrogenase (ICDH)), and/or it may be increased by providing hormonal signals, i.e. adding hormones such as estradiol that enhance the level of endogenous NADPH production.
Similarly, the chaperone can be any chaperone as long as it is capable of facilitating folding of CYP21A2. The present inventors found that the process of the first aspect of the invention can rely only on endogenous chaperones, i.e. additional expression of suitable chaperones is not essential. However, an additional expression improves the production of functional CYP21A2 in the cell and can therefore be beneficial. Thus, in one embodiment of the process of the first aspect of the invention, the one or more chaperones are recombinantly expressed chaperones. In particular, the one or more chaperones may be heterologous chaperones. Exemplary chaperones are the E. coli chaperones GroEL and GroES or other chaperones like DnaK, DnaJ, GrpE, and ClpB as well as small heat shock proteins (sHSP) such as IbpA and IbpB (IbpAB).
Optionally, the same chaperone or one or more additional chaperone(s) to be expressed in the cell is/are capable of folding one or more of above electron transfer proteins.
Recombinant expression refers to the expression of a recombinant gene. Such a gene can be any gene introduced into the cell by methods of genetic engineering and is usually a heterologous gene and/or a gene regulated differently than a possible endogenous counterpart gene in terms of expression. In one embodiment, the recombinant expression is inducible.
In one embodiment of the process of the first aspect of the invention, the process further comprises adding one or more cell permeabilizing agents to the suspension of cells, for example after step b). Cell permeabilizing agents are reagents which increase the permeability of membranes. Examples are organic solvents, such as methanol, acetone or DMSO, detergents such as saponin, Triton X-100 or Tween-20, and EDTA. In particular, polymyxin B can be used as a cell permeabilizing agent. This agent proved to work particularly well in the process of the invention.
In another embodiment of the process of the first aspect of the invention, the process further comprises a step c) of extracting the 21-hydroxylated steroid from the cell and/or the supernatant of the cell (i.e. the buffer or medium the cell is comprised in), for example from a whole cell suspension. The extraction can be done with any solvents or extraction methods known in the art for extracting undissolved compounds. For example, a solvent such as 1-butanol, 2-butanone or chloroform may be used.
In a specific embodiment of the process of the first aspect of the invention, the expression of at least one tryptophanase gene is reduced or abolished in the cell. A tryptophanase or L-tryptophan indole-lyase (EC number 4.1.99.1.) is an enzyme catalyzing the reaction L-tryptophan+H2O=indole+pyruvate+NH3. Accordingly, the reduction or abolishment will lead to the decrease of indol production by the cell, which can improve the process, since indol is an inhibitor of CYP enzymes such as CYP21A2. As tryptophanase genes are generally (but not exclusively) found in prokaryotes, e.g. E. coli, this specific embodiment applies in particular to the embodiment of the process of the first aspect of the invention in which the cell is a prokaryotic cell. In one embodiment, the species of a cell expressing a tryptophansae gene is selected from the group consisting of Aeromonas hydrophila, Bacillus sp., Bacteroides sp., Corynebacterium sp., Enterobacter aerogenes, Enterobacter aerogenes SM-18, Enterobacter sp., Erwinia sp., Escherichia aurescens, Escherichia coli, Fusobacterium necrophorum subsp. Funduliforme, Kluyvera sp., Micrococcus aerogenes, Morganella morganii, Paenibacillus alvei, Paracolobactrum coliforme, Paracolobactrum sp., Pasteurella sp., Photobacterium profundum, Porphyromonas gingivalis, Prevotella intermedia, Proteus vulgaris, Providencia rettgeri, Shigella alkalescens, Sphaerophorus sp., Symbiobacterium thermophilum, Vibrio sp. and a mammalian species such as Homo sapiens and Rattus norvegicus,
In a further embodiment of the process of the first aspect of the invention, the cell further expresses a heterologous or recombinant gene encoding for an enzyme catalyzing a step in the heme biosynthesis pathway, in particular a heterologous hemA (glutamyl tRNA reductase) gene. An example for such cells is E. coli. This will advantageously reduce the need for feeding precursors for the synthesis of the CYP heme, such as the heme precursor δ-aminolevulinic acid, and reduce the costs for the biotransformation of steroids in such cells.
In a particular embodiment of the process of the first aspect of the invention, the nucleic acids encoding for (i) a heterologous CYP21A2 protein or a functional variant thereof, (ii) at least one heterologous electron transfer system capable of transferring electrons to CYP21A2, and optionally (iii) one or more chaperones facilitating folding of CYP21A2 are comprised in one or more expression cassette(s) which is/are integrated into the cell, in particular into its genome. The term “expression cassette” refers to a DNA fragment which comprises a gene operably linked to a regulatory sequence such as a promoter, necessary for gene expression. “Operably linked” refers to the linking of nucleotide regions encoding specific genetic information such that the nucleotide regions are contiguous, the functionality of the region is preserved and will perform relative to the other regions as part of a functional unit. The nucleic acids (i), (ii) and optionally (iii) may each be comprised in individual expression cassettes or in one or more multicistronic expression cassettes. As used herein, the term “multicistronic” means that multiple cistrons, namely, multiple nucleic acids or genes, are operably linked to the same regulatory sequence, e.g. a promoter.
In one embodiment, the nucleic acids encoding for (i) a heterologous CYP21A2 protein or a functional variant thereof, (ii) at least one heterologous electron transfer system capable of transferring electrons to CYP21A2, and optionally (iii) one or more chaperones facilitating folding of CYP21A2 are comprised in an expression vector comprised in the cell. An “expression vector” is a vehicle by means of which DNA fragments that contain nucleic acids encoding for a protein can be introduced into host cells where the nucleic acids can be expressed by the host cell. The nucleic acids (i), (ii) and optionally (iii) may each be comprised in individual expression vectors or in one or more multicistronic expression vectors.
Also, one or more of the nucleic acids (i), (ii) and optionally (iii) may be comprised in an expression cassette which is integrated into the cell genome, whereas the remaining nucleic acids (i), (ii) or optionally (iii) are comprised in an expression expression vector, both as set out above.
In a second aspect, the present invention relates to cell expressing
(i) a heterologous CYP21A2 protein or a functional variant thereof,
(ii) at least one heterologous electron transfer system capable of transferring electrons to CYP21A2, and
(iii) one or more chaperones facilitating folding of CYP21A2.
This cell as well as the cell of the process of the first aspect of the invention can also be described as a cell comprising one or more nucleic acids encoding for
(i) a heterologous CYP21A2 protein or a functional variant thereof,
(ii) at least one heterologous electron transfer system capable of transferring electrons to CYP21A2, and
(iii) one or more chaperones facilitating folding of CYP21A2.
In a particular embodiment, these nucleic acids are comprised in one or more expression cassettes.
The cell of the second aspect is in essence the cell used in the process of the first aspect of the invention and, therefore, further embodiments of the cell of the second aspect of the invention are as described above with respect to the process of the first aspect.
In a third aspect, the present invention relates to a multicistronic expression vector comprising (i) a nucleic acid encoding for a CYP21A2 protein or a functional variant thereof, (ii) one or more nucleic acids encoding for at least one heterologous electron transfer system capable of transferring electrons to CYP21A2, and optionally (iii) one or more nucleic acids encoding for chaperones facilitating folding of CYP21A2.
In a fourth aspect, the present invention relates to a kit comprising
The term “kit” is used herein to mean a collection of all or some of the reagents, materials, and instructions necessary to carry out the process of the first aspect. This includes cell culture medium or buffer (both in dry or liquid form), one or more cell permeabilizing agents, solvents for extracting the steroid and, in particular, the steroid to be hydroxylated, in particular a 3-keto steroid, for example one or more of medrane, deltamedrane, progesterone, 17OH-progesterone, medroxyprogesterone, or 5α-dihydroprogesterone. Furthermore, it is envisaged that all reagents or materials described herein with relation to the process of the first aspect can be part of the kit of the fourth aspect.
In a particular embodiment, the process of the first aspect, the cell of the second aspect, the expression vector of the third aspect and the kit of the fourth aspect do not comprise a further step or component as applicable, especially a heterologous gene or protein, for steroid conversion or production which is not related to the 21-hydroxylation of steroids, particularly as described herein. Optionally, an exception of this may be downstream steps or components related to the further processing of the 21-hydroxylated steroid (such as the conversion from premedrol to medrol) or upstream steps or components related to the production of the steroid to be 21-hydroxylated (such as the production of medrane).
In the following figures and examples, some particular embodiments of the invention are described in more detail. Yet, no limitation of the invention is intended by the details of the particular embodiments. In contrast, the invention pertains to any embodiment which comprises details which are not explicitly mentioned in the embodiments herein, but which the skilled person finds without undue effort.
1.1 Expression/Purification of hCYP21/bCYP21
To show that both human and bovine CYP21 are able to hydroxylate steroids at position 21, in vitro studies with both enzymes were performed. As an exemplary 21-hydroxylation process, medrane was converted to premedrol:
Premedrol (methylhydrocortisone) is a precursor of a highly effective pharmaceutical steroid medrol (methylprednisolone). Medrol is an important drug in therapy of autoimmune diseases, multiple sclerosis and in general for local and systematic treatment of inflammations.
Both enzymes were expressed in the E. coli strain C43(DE3) by coexpression of the E. coli chaperones GroEL/GroES encoded in the vector pGro12. These chaperones ensure a correct protein folding which is important for an incorporation of the heme prosthetic group. In
For an efficient substrate conversion, both isoforms require an electron transfer system which consists of two parts, the cytochrome P450 enzyme itself and one or two electron transfer proteins which are essential for a hydroxylation reaction. Without these transfer proteins, no reaction will take place. Electrons can be transferred to CYP21 for example by the six electron transfer systems listed in Table 2:
For in vitro studies and a verification of a substrate conversion, all redox partners were purified.
In vitro substrate conversions with purified enzymes in a defined buffer and with an NADPH regeneration system revealed that both isoforms together with the here listed electron transfer proteins are able to convert medrane to premedrol very efficiently.
In view of the successful in vitro conversion of steroids, a biotransformation in whole cells was developed.
Generally, in order to perform the hydroxylation in whole cells, the CYP21 as well as the necessary electron transfer proteins were expressed heterologously in Escherichia coli strain C43(DE3). For expression and following conversion, bi- or tricistronic vectors based on the plasmid pET17b were constructed, which carry the genes for the particular CYP21 and the particular redox system.
In particular, E. coli strain C43(DE3) was transformed with vector for whole cell biocatalysis (e.g. p21b_ArET) and the pGro12 which encodes the chaperones GroEL/ES. The culture comprised 200 mL TB medium (+antibiotics ampicillin and kanamycin) in a 2 L Erlenmeyer flask, inoculated with 2 mL seed culture, and was grown at 37° C. Expression was induced at OD 0.5 by addition of 1 mM IPTG, 1 mM δ-aminolevulinic acid, 4 mg/mL arabinose and maintained for 28 h at 27° C. For whole cell biotransformation, cells were harvested by centrifugation and washed with 50 mM potassium phosphate buffer (pH 7.4). Substrate conversion was started with the addition of 400 μM substrate with resting cells in 25 mL potassium phosphate buffer (50 mM) including 2% glycerol, 1 mM IPTG, 1 mM δ-aminolevulinic acid, 4 mg/mL arabinose in 300 mL buffled flasks for 24 h at a cell density of ca. 24 g/L (wet weight). Samples were taken after, e.g., 24 h and measurement was performed via RP-HPLC after steroid extraction with chloroform.
Next to the medrane-to-premedrol conversion, both human and bovine CYP21 were able to hydroxylate all tested 3-ketosteroids which are not yet hydroxylated at position 21. In particular, the following steroid conversions could be shown:
| Number | Date | Country | Kind |
|---|---|---|---|
| 14306740.3 | Oct 2014 | EP | regional |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/EP2015/075096 | 10/29/2015 | WO | 00 |
