3-(3H-imidazo[4,5-B]pyridin-2-yl)-1H-pyrazolo[3,4-B]pyridine and therapeutic uses thereof

Abstract

Azaindazole compounds for treating various diseases and pathologies are disclosed. More particularly, the present invention concerns the use of an azaindazole compound or analogs thereof, in the treatment of disorders characterized by the activation of Wnt pathway signaling (e.g., cancer, abnormal cellular proliferation, angiogenesis, fibrotic disorders, bone or cartilage diseases, and osteoarthritis), the modulation of cellular events mediated by Wnt pathway signaling, as well as genetic diseases and neurological conditions/disorders/diseases due to mutations or dysregulation of the Wnt pathway and/or of one or more of Wnt signaling components. Also provided are methods for treating Wnt-related disease states.

Description

BACKGROUND

Technical Field

This disclosure relates to inhibitors of one or more proteins in the Wnt pathway, including inhibitors of one or more Wnt proteins, and compositions comprising the same. More particularly, it concerns the use of an azaindazole compound or salts or analogs thereof, in the treatment of disorders characterized by the activation of Wnt pathway signaling (e.g., cancer, abnormal cellular proliferation, angiogenesis, fibrotic disorders, bone or cartilage diseases, and osteoarthritis), the modulation of cellular events mediated by Wnt pathway signaling, as well as genetic diseases and neurological conditions/disorders/diseases due to mutations or dysregulation of the Wnt pathway and/or of one or more of Wnt signaling components. Also provided are methods for treating Wnt-related disease states.

Background

The Wnt growth factor family includes more than 10 genes identified in the mouse and at least 19 genes identified in the human. Members of the Wnt family of signaling molecules mediate many short- and long-range patterning processes during invertebrate and vertebrate development. The Wnt signaling pathway is known for its role in the inductive interactions that regulate growth and differentiation, and it also plays roles in the homeostatic maintenance of post-embryonic tissue integrity. Wnt stabilizes cytoplasmic β-catenin, which stimulates the expression of genes including c-myc, c jun, fra-1, and cyclin D1. In addition, misregulation of Wnt signaling can cause developmental defects and is implicated in the genesis of several human cancers. The Wnt pathway has also been implicated in the maintenance of stem or progenitor cells in a growing list of adult tissues including skin, blood, gut, prostate, muscle, and the nervous system.

SUMMARY

The present disclosure provides methods and reagents, involving contacting a cell with an agent, such as an azaindazole compound, in a sufficient amount to antagonize a Wnt activity, e.g., to reverse or control an aberrant growth state or correct a genetic disorder due to mutations in Wnt signaling components.

Some embodiments disclosed herein include Wnt inhibitors containing an azaindazole core. Other embodiments disclosed herein include pharmaceutical compositions and methods of treatment using these compounds.

One embodiment disclosed herein includes a compound having the structure of Formula I:

• • as well as prodrugs and pharmaceutically acceptable salts thereof.

In some embodiments of Formula (I):

• • R¹ is selected from the group consisting of -heteroaryl(R⁴)_q and -heterocyclyl(R⁵)_h;
  • R² is selected from the group consisting of H and halide;
  • R³ is selected from the group consisting of H, -heteroaryl(R⁶)_q, -heterocyclyl(R⁷)_h, and -aryl(R⁸)_k;
  • each R⁴ is one substituent attached to the heteroaryl and is independently selected from the group consisting of halide, —(C_1-6 alkyl), —(C_1-4 alkylene)_pheterocyclyl(R⁹)_h, —(C_1-4 alkylene)_pcarbocyclyl(R¹⁰)_j, —(C_1-4 alkylene)_paryl(R¹¹)_k, —NHC(═O)R¹², —NR¹³R¹⁴, —(C_1-6 alkylene)NR¹⁵R¹⁶, and —OR²²;
  • each R⁵ is one substituent attached to the heterocyclyl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R⁶ is one substituent attached to the heteroaryl and is independently selected from the group consisting of —(C_1-6 alkyl), halide, —CF₃, —OCH₃, —CN, and —C(═O)R¹⁷;
  • each R⁷ is one substituent attached to the heterocyclyl and is independently selected from the group consisting of —(C_1-6 alkyl), halide, —CF₃, —CN, and —OCH₃;
  • each R⁸ is one substituent attached to the aryl and is independently selected from the group consisting of —(C_1-6 alkyl), halide, —CF₃, —CN, —OCH₃, —(C_1-6 alkylene)_pNHSO₂R¹⁷, —NR¹³(C_1-6 alkylene)NR¹³R¹⁴, —(C_1-6 alkylene)_pNR¹³R¹⁴, and —OR²⁵;
  • each R⁹ is one substituent attached to the heterocyclyl and is independently selected from the group consisting of amino, —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R¹⁰ is one substituent attached to the carbocyclyl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R¹¹ is one substituent attached to the aryl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R¹² is independently selected from the group consisting of —(C_1-9 alkyl), -heteroaryl(R¹⁸)_q, -aryl(R¹⁹)_k, —CH₂aryl(R¹⁹)_k, -carbocyclyl(R²⁰)_j, —CH₂carbocyclyl(R²⁰)_j, —(C_1-4 alkylene)_pNR²³R²⁴, -heterocyclyl(R²¹)_h, and —CH₂heterocyclyl(R²¹)_h;
  • each R¹³ is independently selected from the group consisting of H and —(C_1-6 alkyl);
  • each R¹⁴ is independently selected from the group consisting of H, —(C_1-6 alkyl), —CH₂aryl(R¹⁹)_k, and —CH₂carbocyclyl(R²⁰)_j;
  • each R¹⁵ is independently selected from the group consisting of H and —(C_1-6 alkyl);
  • each R¹⁶ is independently selected from the group consisting of H, —(C_1-6 alkyl), —CH₂aryl(R¹⁹)_k, and —CH₂carbocyclyl(R²⁰)_j;
  • each R¹⁷ is a —(C_1-6 alkyl);
  • each R¹⁸ is one substituent attached to the heteroaryl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R¹⁹ is one substituent attached to the aryl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R²⁰ is one substituent attached to the carbocyclyl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R²¹ is one substituent attached to the heterocyclyl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R²² is independently selected from the group consisting of H, —(C_1-6 alkyl), —(C_1-4 alkylene)_pheterocyclyl(R²¹)_h, —(C_1-4 alkylene)_pcarbocyclyl(R²⁰)_j, —(C_1-4 alkylene)_paryl(R¹⁹)_k, and —(C_1-6 alkylene)_pNR²³R²⁴;
  • each R²³ is independently selected from the group consisting of H and —(C_1-6 alkyl);
  • each R²⁴ is independently selected from the group consisting of H and —(C_1-6 alkyl);
  • each R²⁵ is independently selected from the group consisting of H, —(C_1-6 alkyl), —(C_1-4 alkylene)_pheterocyclyl(R²¹)_h, and —(C_1-6 alkylene)_pNR²³R²⁴;
  • each p is independently 0 or 1;
  • each q is independently 0 to 4;
  • each h is independently 0 to 10;
  • each k is independently 0 to 5; and
  • each j is independently 0 to 12.

Some embodiments include stereoisomers and pharmaceutically acceptable salts of a compound of Formula (I).

Some embodiments include pro-drugs of a compound of Formula (I).

Some embodiments of the present disclosure include pharmaceutical compositions comprising a compound of Formula (I) and a pharmaceutically acceptable carrier, diluent, or excipient.

Other embodiments disclosed herein include methods of inhibiting one or more members of the Wnt pathway, including one or more Wnt proteins by administering to a patient affected by a disorder or disease in which aberrant Wnt signaling is implicated, such as cancer and other diseases associated with abnormal angiogenesis, cellular proliferation, cell cycling and mutations in Wnt signaling components, a compound according to Formula (I). Accordingly, the compounds and compositions provided herein can be used to treat cancer, to reduce or inhibit angiogenesis, to reduce or inhibit cellular proliferation and correct a genetic disorder due to mutations in Wnt signaling components.

Non-limiting examples of diseases which can be treated with the compounds and compositions provided herein include a variety of cancers, diabetic retinopathy, pulmonary fibrosis, rheumatoid arthritis, sepsis, anklyosing spondylitis, psoriasis, scleroderma, mycotic and viral infections, osteochondrodysplasia, Alzheimer's disease, lung disease, bone/osteoporotic (wrist, spine, shoulder and hip) fractures, articular cartilage (chondral) defects, degenerative disc disease (or intervertebral disc degeneration), polyposis coli, osteoporosis-pseudoglioma syndrome, familial exudative vitreoretinopathy, retinal angiogenesis, early coronary disease, tetra-Amelia syndrome, Müllerian-duct regression and virilization, SERKAL syndrome, diabetes mellitus type 2, Fuhrmann syndrome, Al-Awadi/Raas-Rothschild/Schinzel phocomelia syndrome, odonto-onycho-dermal dysplasia, obesity, split-hand/foot malformation, caudal duplication syndrome, tooth agenesis, Wilms tumor, skeletal dysplasia, focal dermal hypoplasia, autosomal recessive anonychia, neural tube defects, alpha-thalassemia (ATRX) syndrome, fragile X syndrome, ICF syndrome, Angelman syndrome, Prader-Willi syndrome, Beckwith-Wiedemann Syndrome, Norrie disease, and Rett syndrome.

Some embodiments of the present disclosure include methods to prepare compounds of Formula (I).

It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the invention, as claimed.

DETAILED DESCRIPTION

Provided herein are compositions and methods for inhibiting one or more members of the Wnt pathway, including one or more Wnt proteins. Other Wnt inhibitors and methods for using the same are disclosed in U.S. application Ser. Nos. 12/852,706; 12/968,505; 13/552,188; 13/800,963; 13/855,874; 13/887,177 13/938,691; 13/938,692; 14/019,103; 14/019,147; 14/019,940; 14/149,948; 14/178,749; 14/331,427; and Ser. No. 14/334,005; and U.S. Provisional Application Ser. Nos. 61/232,603; 61/288,544; 61/305,459; 61/620,107; 61/642,915; and 61/750,221, all of which are incorporated by reference in their entirety herein.

Some embodiments provided herein relate to a method for treating a disease or disorder including, but not limited to, cancers, diabetic retinopathy, pulmonary fibrosis, rheumatoid arthritis, sepsis, anklyosing spondylitis, psoriasis, scleroderma, mycotic and viral infections, bone and cartilage diseases, Alzheimer's disease, lung disease, osteoarthritis, bone/osteoporotic (wrist, spine, shoulder and hip) fractures, articular cartilage (chondral) defects, degenerative disc disease (or intervertebral disc degeneration), polyposis coli, bone density and vascular defects in the eye (Osteoporosis-pseudoglioma Syndrome, OPPG) and other eye diseases or syndromes associated with defects or damaged photoreceptors, familial exudative vitreoretinopathy, retinal angiogenesis, early coronary disease, tetra-amelia, Müllerian-duct regression and virilization, SERKAL syndrome, type II diabetes, Fuhrmann syndrome, Al-Awadi/Raas-Rothschild/Schinzel phocomelia syndrome, odonto-onycho-dermal dysplasia, obesity, split-hand/foot malformation, caudal duplication, tooth agenesis, Wilms tumor, skeletal dysplasia, focal dermal hypoplasia, autosomal recessive anonychia, neural tube defects, alpha-thalassemia (ATRX) syndrome, fragile X syndrome, ICF syndrome, Angelman's syndrome, Prader-Willi syndrome, Beckwith-Wiedemann Syndrome, Norrie disease, and Rett syndrome.

In some embodiments, non-limiting examples of bone and cartilage diseases which can be treated with the compounds and compositions provided herein include bone spur (osteophytes), craniosynostosis, fibrodysplasia ossificans progressive, fibrous dysplasia, giant cell tumor of bone, hip labral tear, meniscal tears, bone/osteoporotic (wrist, spine, shoulder and hip) fractures, articular cartilage (chondral) defects, degenerative disc disease (or intervertebral disc degeneration), osteochondritis dissecans, osteochondroma (bone tumor), osteopetrosis, relapsing polychondritis, and Salter-Harris fractures.

In some embodiments, pharmaceutical compositions are provided that are effective for treatment of a disease of an animal, e.g., a mammal, caused by the pathological activation or mutations of the Wnt pathway. The composition includes a pharmaceutically acceptable carrier and a compound as described herein.

Definitions

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as is commonly understood by one of ordinary skill in the art to which this disclosure belongs. All patents, applications, published applications, and other publications are incorporated by reference in their entirety. In the event that there is a plurality of definitions for a term herein, those in this section prevail unless stated otherwise.

As used herein, “alkyl” means a branched, or straight chain chemical group containing only carbon and hydrogen, such as methyl, ethyl, n-propyl, iso-propyl, n-butyl, iso-butyl, sec-butyl, tert-butyl, n-pentyl, iso-pentyl, sec-pentyl and neo-pentyl. Alkyl groups can either be unsubstituted or substituted with one or more substituents. Alkyl groups can be saturated or unsaturated (e.g., containing —C═C— or —C≡C— subunits), at one or several positions. In some embodiments, alkyl groups include 1 to 9 carbon atoms (for example, 1 to 6 carbon atoms, 1 to 4 carbon atoms, or 1 to 2 carbon atoms).

As used herein, “alkylene” means a bivalent branched, or straight chain chemical group containing only carbon and hydrogen, such as methylene, ethylene, n-propylene, iso-propylene, n-butylene, iso-butylene, sec-butylene, tert-butylene, n-pentylene, iso-pentylene, sec-pentylene and neo-pentylene. Alkylene groups can either be unsubstituted or substituted with one or more substituents. Alkylene groups can be saturated or unsaturated (e.g., containing —C═C— or —C≡C— subunits), at one or several positions. In some embodiments, alkylene groups include 1 to 9 carbon atoms (for example, 1 to 6 carbon atoms, 1 to 4 carbon atoms, or 1 to 2 carbon atoms).

As used herein, “carbocyclyl” means a cyclic ring system containing only carbon atoms in the ring system backbone, such as cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, and cyclohexenyl. Carbocyclyls may include multiple fused rings. Carbocyclyls may have any degree of saturation provided that at least one ring in the ring system is not aromatic. Carbocyclyl groups can either be unsubstituted or substituted with one or more substituents. In some embodiments, carbocyclyl groups include 3 to 10 carbon atoms, for example, 3 to 6 carbon atoms.

As used herein, “lower alkyl” means a subset of alkyl having 1 to 3 carbon atoms, which is linear or branched. Examples of lower alkyls include methyl, ethyl, n-propyl and isopropyl. Likewise, radicals using the terminology “lower” refer to radicals having 1 to about 3 carbons in the alkyl portion of the radical.

As used herein, “aryl” means a mono-, bi-, tri- or polycyclic group with only carbon atoms present in the ring backbone having 5 to 14 ring atoms, alternatively 5, 6, 9, or 10 ring atoms; and having 6, 10, or 14 pi electrons shared in a cyclic array; wherein at least one ring in the system is aromatic. Aryl groups can either be unsubstituted or substituted with one or more substituents. Examples of aryl include phenyl, naphthyl, tetrahydronaphthyl, 2,3-dihydro-1H-indenyl, and others. In some embodiments, the aryl is phenyl.

As used herein, “arylalkyl” means an aryl-alkyl-group in which the aryl and alkyl moieties are as previously described. In some embodiments, arylalkyl groups contain a C_1-4alkyl moiety. Exemplary arylalkyl groups include benzyl and 2-phenethyl.

As used herein, the term “heteroaryl” means a mono-, bi-, tri- or polycyclic group having 5 to 14 ring atoms, alternatively 5, 6, 9, or 10 ring atoms; and having 6, 10, or 14 pi electrons shared in a cyclic array; wherein at least one ring in the system is aromatic, and at least one ring in the system contains one or more heteroatoms independently selected from the group consisting of N, O, and S. Heteroaryl groups can either be unsubstituted or substituted with one or more substituents. Examples of heteroaryl include thienyl, pyridinyl, furyl, oxazolyl, oxadiazolyl, pyrrolyl, imidazolyl, triazolyl, thiodiazolyl, pyrazolyl, isoxazolyl, thiadiazolyl, pyranyl, pyrazinyl, pyrimidinyl, pyridazinyl, triazinyl, thiazolyl benzothienyl, benzoxadiazolyl, benzofuranyl, benzimidazolyl, benzotriazolyl, cinnolinyl, indazolyl, indolyl, isoquinolinyl, isothiazolyl, naphthyridinyl, purinyl, thienopyridinyl, pyrido[2,3-d]pyrimidinyl, pyrrolo[2,3-b]pyridinyl, quinazolinyl, quinolinyl, thieno[2,3-c]pyridinyl, pyrazolo[3,4-b]pyridinyl, pyrazolo[3,4-c]pyridinyl, pyrazolo[4,3-c]pyridine, pyrazolo[4,3-b]pyridinyl, tetrazolyl, chromane, 2,3-dihydrobenzo[b][1,4]dioxine, benzo[d][1,3]dioxole, 2,3-dihydrobenzofuran, 2,3-dihydrobenzo[b][1,4]oxathiine, and others. In some embodiments, the heteroaryl is selected from thienyl, pyridinyl, furyl, pyrazolyl, imidazolyl, pyranyl, pyrazinyl, and pyrimidinyl.

As used herein, “halo”, “halide” or “halogen” is a chloro, bromo, fluoro, or iodo atom radical. In some embodiments, a halo is a chloro, bromo or fluoro. For example, a halide can be fluoro.

As used herein, “haloalkyl” means a hydrocarbon substituent, which is a linear or branched, alkyl, alkenyl or alkynyl substituted with one or more chloro, bromo, fluoro, and/or iodo atom(s). In some embodiments, a haloalkyl is a fluoroalkyls, wherein one or more of the hydrogen atoms have been substituted by fluoro. In some embodiments, haloalkyls are of 1 to about 3 carbons in length (e.g., 1 to about 2 carbons in length or 1 carbon in length). The term “haloalkylene” means a diradical variant of haloalkyl, and such diradicals may act as spacers between radicals, other atoms, or between a ring and another functional group.

As used herein, “heterocyclyl” means a nonaromatic cyclic ring system comprising at least one heteroatom in the ring system backbone. Heterocyclyls may include multiple fused rings. Heterocyclyls may be substituted or unsubstituted with one or more substituents. In some embodiments, heterocycles have 5-7 members. In six membered monocyclic heterocycles, the heteroatom(s) are selected from one to three of O, N or S, and wherein when the heterocycle is five membered, it can have one or two heteroatoms selected from O, N, or S. Examples of heterocyclyl include azirinyl, aziridinyl, azetidinyl, oxetanyl, thietanyl, 1,4,2-dithiazolyl, dihydropyridinyl, 1,3-dioxanyl, 1,4-dioxanyl, 1,3-dioxolanyl, morpholinyl, thiomorpholinyl, piperazinyl, pyranyl, pyrrolidinyl, tetrahydrofuryl, tetrahydropyridinyl, oxazinyl, thiazinyl, thiinyl, thiazolidinyl, isothiazolidinyl, oxazolidinyl, isoxazolidinyl, piperidinyl, pyrazolidinyl imidazolidinyl, thiomorpholinyl, and others. In some embodiments, the heterocyclyl is selected from azetidinyl, morpholinyl, piperazinyl, pyrrolidinyl, and tetrahydropyridinyl.

As used herein, “monocyclic heterocyclyl” means a single nonaromatic cyclic ring comprising at least one heteroatom in the ring system backbone. Heterocyclyls may be substituted or unsubstituted with one or more substituents. In some embodiments, heterocycles have 5-7 members. In six membered monocyclic heterocycles, the heteroatom(s) are selected from one to three of O, N or S, and wherein when the heterocycle is five membered, it can have one or two heteroatoms selected from O, N, or S. Examples of heterocyclyl include azirinyl, aziridinyl, azetidinyl, oxetanyl, thietanyl, 1,4,2-dithiazolyl, dihydropyridinyl, 1,3-dioxanyl, 1,4-dioxanyl, 1,3-dioxolanyl, morpholinyl, thiomorpholinyl, piperazinyl, pyranyl, pyrrolidinyl, tetrahydrofuryl, tetrahydropyridinyl, oxazinyl, thiazinyl, thiinyl, thiazolidinyl, isothiazolidinyl, oxazolidinyl, isoxazolidinyl, piperidinyl, pyrazolidinyl imidazolidinyl, thiomorpholinyl, and others.

The term “substituted” refers to moieties having substituents replacing a hydrogen on one or more non-hydrogen atoms of the molecule. It will be understood that “substitution” or “substituted with” includes the implicit proviso that such substitution is in accordance with permitted valence of the substituted atom and the substituent, and that the substitution results in a stable compound, e.g., which does not spontaneously undergo transformation such as by rearrangement, cyclization, elimination, etc. Substituents can include, for example, —(C_1-9 alkyl) optionally substituted with one or more of hydroxyl, —NH₂, —NH(C_1-3 alkyl), and —N(C_1-3 alkyl)₂; —(C_1-9 haloalkyl); a halide; a hydroxyl; a carbonyl [such as —C(O)OR, and —C(O)R]; a thiocarbonyl [such as —C(S)OR, —C(O)SR, and —C(S)R]; —(C_1-9 alkoxyl) optionally substituted with one or more of halide, hydroxyl, —NH₂, —NH(C_1-3 alkyl), and —N(C_1-3 alkyl)₂; —OPO(OH)₂; a phosphonate [such as —PO(OH)₂ and —PO(OR′)₂]; —OPO(OR′)R″; —NRR′; —C(O)NRR′; —C(NR)NR′R″; —C(NR′)R″; a cyano; a nitro; an azido; —SH; —S—R; —OSO₂(OR); a sulfonate [such as —SO₂(OH) and —SO₂(OR)]; —SO₂NR′R″; and —SO₂R; in which each occurrence of R, R′ and R″ are independently selected from H; —(C_1-9 alkyl); C_6-10 aryl optionally substituted with from 1-3R′″; 5-10 membered heteroaryl having from 1-4 heteroatoms independently selected from N, O, and S and optionally substituted with from 1-3 R′″; C_3-7 carbocyclyl optionally substituted with from 1-3 R′″; and 3-8 membered heterocyclyl having from 1-4 heteroatoms independently selected from N, O, and S and optionally substituted with from 1-3 R′″; wherein each R′″ is independently selected from —(C_1-6 alkyl), —(C_1-6 haloalkyl), a halide (e.g., F), a hydroxyl, —C(O)OR, —C(O)R, —(C_1-6 alkoxyl), —NRR′, —C(O)NRR′, and a cyano, in which each occurrence of R and R′ is independently selected from H and —(C_1-6 alkyl). In some embodiments, the substituent is selected from —(C_1-6 alkyl), —(C_1-6 haloalkyl), a halide (e.g., F), a hydroxyl, —C(O)OR, —C(O)R, —(C_1-6 alkoxyl), —NRR′, —C(O)NRR′, and a cyano, in which each occurrence of R and R′ is independently selected from H and —(C_1-6 alkyl).

As used herein, when two groups are indicated to be “linked” or “bonded” to form a “ring”, it is to be understood that a bond is formed between the two groups and may involve replacement of a hydrogen atom on one or both groups with the bond, thereby forming a carbocyclyl, heterocyclyl, aryl, or heteroaryl ring. The skilled artisan will recognize that such rings can and are readily formed by routine chemical reactions. In some embodiments, such rings have from 3-7 members, for example, 5 or 6 members.

The skilled artisan will recognize that some structures described herein may be resonance forms or tautomers of compounds that may be fairly represented by other chemical structures, even when kinetically, the artisan recognizes that such structures are only a very small portion of a sample of such compound(s). Such compounds are clearly contemplated within the scope of this disclosure, though such resonance forms or tautomers are not represented herein.

The compounds provided herein may encompass various stereochemical forms. The compounds also encompass diastereomers as well as optical isomers, e.g., mixtures of enantiomers including racemic mixtures, as well as individual enantiomers and diastereomers, which arise as a consequence of structural asymmetry in certain compounds. Separation of the individual isomers or selective synthesis of the individual isomers is accomplished by application of various methods which are well known to practitioners in the art. Unless otherwise indicated, when a disclosed compound is named or depicted by a structure without specifying the stereochemistry and has one or more chiral centers, it is understood to represent all possible stereoisomers of the compound.

The term “administration” or “administering” refers to a method of providing a dosage of a compound or pharmaceutical composition to a vertebrate or invertebrate, including a mammal, a bird, a fish, or an amphibian, where the method is, e.g., orally, subcutaneously, intravenously, intralymphatic, intranasally, topically, transdermally, intraperitoneally, intramuscularly, intrapulmonarilly, vaginally, rectally, ontologically, neuro-otologically, intraocularly, subconjuctivally, via anterior eye chamber injection, intravitreally, intraperitoneally, intrathecally, intracystically, intrapleurally, via wound irrigation, intrabuccally, intra-abdominally, intra-articularly, intra-aurally, intrabronchially, intracapsularly, intrameningeally, via inhalation, via endotracheal or endobronchial instillation, via direct instillation into pulmonary cavities, intraspinally, intrasynovially, intrathoracically, via thoracostomy irrigation, epidurally, intratympanically, intracisternally, intravascularly, intraventricularly, intraosseously, via irrigation of infected bone, or via application as part of any admixture with a prosthetic device. The method of administration can vary depending on various factors, e.g., the components of the pharmaceutical composition, the site of the disease, the disease involved, and the severity of the disease.

A “diagnostic” as used herein is a compound, method, system, or device that assists in the identification or characterization of a health or disease state. The diagnostic can be used in standard assays as is known in the art.

The term “mammal” is used in its usual biological sense. Thus, it specifically includes humans, cattle, horses, monkeys, dogs, cats, mice, rats, cows, sheep, pigs, goats, and non-human primates, but also includes many other species.

The term “pharmaceutically acceptable carrier”, “pharmaceutically acceptable diluent” or “pharmaceutically acceptable excipient” includes any and all solvents, co-solvents, complexing agents, dispersion media, coatings, isotonic and absorption delaying agents and the like which are not biologically or otherwise undesirable. The use of such media and agents for pharmaceutically active substances is well known in the art. Except insofar as any conventional media or agent is incompatible with the active ingredient, its use in the therapeutic compositions is contemplated. Supplementary active ingredients can also be incorporated into the compositions. In addition, various adjuvants such as are commonly used in the art may be included. These and other such compounds are described in the literature, e.g., in the Merck Index, Merck & Company, Rahway, N.J. Considerations for the inclusion of various components in pharmaceutical compositions are described, e.g., in Gilman et al. (Eds.) (2010); Goodman and Gilman's: The Pharmacological Basis of Therapeutics, 12th Ed., The McGraw-Hill Companies.

The term “pharmaceutically acceptable salt” refers to salts that retain the biological effectiveness and properties of the compounds provided herein and, which are not biologically or otherwise undesirable. In many cases, the compounds provided herein are capable of forming acid and/or base salts by virtue of the presence of amino and/or carboxyl groups or groups similar thereto. Many such salts are known in the art, for example, as described in WO 87/05297. Pharmaceutically acceptable acid addition salts can be formed with inorganic acids and organic acids. Inorganic acids from which salts can be derived include, for example, hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid, and the like. Organic acids from which salts can be derived include, for example, acetic acid, propionic acid, glycolic acid, pyruvic acid, oxalic acid, maleic acid, malonic acid, succinic acid, fumaric acid, tartaric acid, citric acid, benzoic acid, cinnamic acid, mandelic acid, methanesulfonic acid, ethanesulfonic acid, p-toluenesulfonic acid, salicylic acid, and the like. Pharmaceutically acceptable base addition salts can be formed with inorganic and organic bases. Inorganic bases from which salts can be derived include, for example, sodium, potassium, lithium, ammonium, calcium, magnesium, iron, zinc, copper, manganese, aluminum, and the like; particularly preferred are the ammonium, potassium, sodium, calcium, and magnesium salts. Organic bases from which salts can be derived include, for example, primary, secondary, and tertiary amines, substituted amines including naturally occurring substituted amines, cyclic amines, basic ion exchange resins, and the like, specifically such as isopropylamine, trimethylamine, diethylamine, triethylamine, tripropylamine, and ethanolamine.

“Solvate” refers to the compound formed by the interaction of a solvent and a compound as provided herein or a salt thereof. Suitable solvates are pharmaceutically acceptable solvates including hydrates.

“Patient” as used herein, means a human or a non-human mammal, e.g., a dog, a cat, a mouse, a rat, a cow, a sheep, a pig, a goat, a non-human primate, or a bird, e.g., a chicken, as well as any other vertebrate or invertebrate. In some embodiments, the patient is a human.

A “therapeutically effective amount” or “pharmaceutically effective amount” of a compound as provided herein is one which is sufficient to achieve the desired physiological effect and may vary according to the nature and severity of the disease condition, and the potency of the compound. “Therapeutically effective amount” is also intended to include one or more of the compounds of Formula I in combination with one or more other agents that are effective to treat the diseases and/or conditions described herein. The combination of compounds can be a synergistic combination. Synergy, as described, for example, by Chou and Talalay, Advances in Enzyme Regulation (1984), 22, 27-55, occurs when the effect of the compounds when administered in combination is greater than the additive effect of the compounds when administered alone as a single agent. In general, a synergistic effect is most clearly demonstrated at sub-optimal concentrations of the compounds. It will be appreciated that different concentrations may be employed for prophylaxis than for treatment of an active disease. This amount can further depend upon the patient's height, weight, sex, age and medical history.

A therapeutic effect relieves, to some extent, one or more of the symptoms of the disease.

“Treat,” “treatment,” or “treating,” as used herein refers to administering a compound or pharmaceutical composition as provided herein for therapeutic purposes. The term “therapeutic treatment” refers to administering treatment to a patient already suffering from a disease thus causing a therapeutically beneficial effect, such as ameliorating existing symptoms, ameliorating the underlying metabolic causes of symptoms, postponing or preventing the further development of a disorder, and/or reducing the severity of symptoms that will or are expected to develop.

“Drug-eluting” and/or controlled release as used herein refers to any and all mechanisms, e.g., diffusion, migration, permeation, and/or desorption by which the drug(s) incorporated in the drug-eluting material pass therefrom over time into the surrounding body tissue.

“Drug-eluting material” and/or controlled release material as used herein refers to any natural, synthetic or semi-synthetic material capable of acquiring and retaining a desired shape or configuration and into which one or more drugs can be incorporated and from which incorporated drug(s) are capable of eluting over time.

“Elutable drug” as used herein refers to any drug or combination of drugs having the ability to pass over time from the drug-eluting material in which it is incorporated into the surrounding areas of the body.

The term “comprising” as used herein is synonymous with “including,” “containing,” or “characterized by,” and is inclusive or open-ended and does not exclude additional, unrecited elements or method steps.

Compounds

The compounds and compositions described herein can be used as anti-proliferative agents, e.g., anti-cancer and anti-angiogenesis agents, and/or as inhibitors of the Wnt signaling pathway, e.g., for treating diseases or disorders associated with aberrant Wnt signaling. In addition, the compounds can be used as inhibitors of one or more kinases, kinase receptors, or kinase complexes. Such compounds and compositions are also useful for controlling cellular proliferation, differentiation, and/or apoptosis.

Some embodiments of the present disclosure include compounds of Formula I:

or salts, pharmaceutically acceptable salts, or prodrugs thereof.

In some embodiments, R¹ is selected from the group consisting of -pyridinyl(R⁴) and -pyrimidinyl(R⁵).

In some embodiments, R¹ is selected from the group consisting of -heteroaryl(R⁴)_q and -heterocyclyl(R⁵)_h.

In some embodiments, R¹ is selected from the group consisting of -piperidinyl(R⁵)_h and -tetrahydropyridinyl(R⁵)_h.

In some embodiments, R¹ is selected from the group consisting of -pyridinyl(R⁴)_q, -pyrimidinyl(R⁴)_q, -pyrazinyl(R⁴)_q, -pyrazolyl(R⁴)_q, and -imidazolyl(R⁴)_q.

In some embodiments, R² is selected from the group consisting of H and halide.

In some embodiments, R³ is selected from the group consisting of -heteroaryl(R⁶)_q, -heterocyclyl(R⁷)_h, and -aryl(R⁸)_k.

In some embodiments, R³ is selected from the group consisting of H, -heteroaryl(R⁶)_q, -heterocyclyl(R⁷)_h, and -aryl(R⁸)_k.

In some embodiments, R³ is selected from the group consisting of -pyridinyl(R⁶)_q, -imidazolyl(R⁶)_q, -furanyl(R⁶)_q, -thiophenyl(R⁶)_q, -piperidinyl(R⁷)_h, -piperazinyl(R⁷)_h, and -phenyl(R⁸)_k.

In some embodiments, R⁴ is one substituent attached to the pyridinyl and is independently selected from the group consisting of H, halide, —(C_1-6 alkyl), —(C_1-4 alkylene)_pheterocyclyl(R⁹)_h, —(C_1-4 alkylene)_pcarbocyclyl(R¹⁰)_j, —(C_1-4 alkylene)_paryl(R¹¹)_k, —NHC(═O)R¹², —NR¹³R¹⁴, and —(C_1-6 alkylene)NR¹⁵R¹⁶.

In some embodiments, each R⁴ is one substituent attached to the heteroaryl and is independently selected from the group consisting of halide, —(C_1-6 alkyl), —(C_1-4 alkylene)_pheterocyclyl(R⁹)_h, —(C_1-4 alkylene)_pcarbocyclyl(R¹⁰)_j, —(C_1-4 alkylene)_paryl(R¹¹)_k, —NHC(═O)R¹², —NR¹³R¹⁴, —(C_1-6 alkylene)NR¹⁵R¹⁶, and —OR²².

In some embodiments, each R⁴ is one substituent attached to the heteroaryl and is independently selected from the group consisting of F, -Me, -Et, —(CH₂)heterocyclyl(R⁹)_h, -heterocyclyl(R⁹)_h, —(CH₂)carbocyclyl(R¹⁰)_j, —(CH₂)aryl(R¹¹)_k, —NHC(═O)(C_1-5 alkyl), —NHC(═O)phenyl(R¹⁹)_k, —NHC(═O)(CH₂)phenyl(R¹⁹)_k, —NHC(═O)carbocyclyl(R²⁰)_j, —NHC(═O)(CH₂)heterocyclyl(R²¹)_h, —NH₂, —N(C_1-3 alkyl)₂, —NH(C_1-4 alkyl), —(CH₂)N(C_1-3 alkyl)₂, —(CH₂)NH(C_1-4 alkyl), —OH, —O(C_1-3 alkyl), —Ocarbocyclyl(R²⁰)_j, —Oheterocyclyl(R²)_h, —O(CH₂CH₂)heterocyclyl(R²¹)_h, —O(CH₂CH₂)N(C_1-3 alkyl)₂, and —O(CH₂)phenyl(R¹⁹)_k.

In some embodiments, R⁵ is one substituent attached to the pyrimidinyl and is independently selected from the group consisting of H, halide, —(C_1-6 alkyl), —(C_1-4 alkylene)_pheterocyclyl(R⁹)_h, —(C_1-4 alkylene)_pcarbocyclyl(R¹⁰)_j, —(C_1-4 alkylene)_paryl(R¹¹)_k, —NHC(═O)R¹², —NR¹³R¹⁴, and —(C_1-6 alkylene)NR¹⁵R¹⁶.

In some embodiments, each R⁵ is one substituent attached to the heterocyclyl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN.

In some embodiments, each R⁶ is one substituent attached to the heteroaryl and is independently selected from the group consisting of H, —(C_1-6 alkyl), halide, —CF₃, —OCH₃, —CN, and —C(═O)R¹⁷.

In some embodiments, each R⁶ is one substituent attached to the heteroaryl and is independently selected from the group consisting of —(C_1-6 alkyl), halide, —CF₃, —OCH₃, —CN, and —C(═O)R¹⁷.

In some embodiments, each R⁶ is one substituent attached to the heteroaryl and is independently selected from the group consisting of -Me, -Et, F, —CF₃, —OCH₃, —CN, and —C(═O)(C_1-3 alkyl).

In some embodiments, each R⁷ is one substituent attached to the heterocyclyl and is independently selected from the group consisting of H, —(C_1-6 alkyl), halide, —CF₃, —CN, and —OCH₃.

In some embodiments, each R⁷ is one substituent attached to the heterocyclyl and is independently selected from the group consisting of —(C_1-6 alkyl), halide, —CF₃, —CN, and —OCH₃.

In some embodiments, each R⁸ is one substituent attached to the aryl and is independently selected from the group consisting of H, —(C_1-6 alkyl), halide, —CF₃, —CN, —OCH₃, —(C_1-6 alkylene)_pNHSO₂R¹⁷, —NR¹³(C_1-6 alkylene)NR¹³R¹⁴, and —(C_1-6 alkylene)_pNR¹³R¹⁴.

In some embodiments, each R⁸ is one substituent attached to the aryl and is independently selected from the group consisting of —(C_1-6 alkyl), halide, —CF₃, —CN, —OCH₃, —(C_1-6 alkylene)_pNHSO₂R¹⁷, —NR¹³(C_1-6 alkylene)NR¹³R¹⁴, —(C_1-6 alkylene)_pNR¹³R¹⁴, and —OR²⁵.

In some embodiments, each R⁸ is one substituent attached to the aryl and is independently selected from the group consisting of -Me, -Et, F, —CF₃, —CN, —OCH₃, —(CH₂CH₂)NHSO₂(C_1-3 alkyl), —NH(CH₂CH₂)N(C_1-3 alkyl)₂, —OH, —O(C_1-3 alkyl), —O(CH₂CH₂)heterocyclyl(R²¹)_h, and —O(CH₂CH₂)N(C_1-3 alkyl)₂.

In some embodiments, each R⁹ is one substituent attached to the heterocyclyl and is independently selected from the group consisting of H, —(C_1-4 alkyl), halide, —CF₃, and —CN.

In some embodiments, each R⁹ is one substituent attached to the heterocyclyl and is independently selected from the group consisting of amino, —(C_1-4 alkyl), halide, —CF₃, and —CN.

In some embodiments, each R⁹ is one substituent attached to the heterocyclyl and is independently selected from the group consisting of amino, Me, Et, F, Cl, and —CF₃.

In some embodiments, each R¹⁰ is one substituent attached to the carbocyclyl and is independently selected from the group consisting of H, —(C_1-4 alkyl), halide, —CF₃, and —CN.

In some embodiments, each R¹⁰ is one substituent attached to the carbocyclyl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN.

In some embodiments, each R¹⁰ is one substituent attached to the carbocyclyl and is independently selected from the group consisting of Me, Et, F, Cl, and —CF₃.

In some embodiments, each R¹¹ is one substituent attached to the aryl and is independently selected from the group consisting of H, —(C_1-4 alkyl), halide, —CF₃, and —CN.

In some embodiments, each R¹¹ is one substituent attached to the aryl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN.

In some embodiments, each R¹¹ is one substituent attached to the aryl and is independently selected from the group consisting of Me, Et, F, Cl, and —CF₃.

In some embodiments, each R¹² is independently selected from the group consisting of —(C_1-9 alkyl), -heteroaryl(R¹⁸)_q, -aryl(R¹⁹)_k, —CH₂aryl(R¹⁹)_k, -carbocyclyl(R²⁰)_j, and —CH₂carbocyclyl(R²⁰)_j.

In some embodiments, each R¹² is independently selected from the group consisting of —(C_1-9 alkyl), -heteroaryl(R¹⁸)_q, -aryl(R¹⁹)_k, —CH₂aryl(R¹⁹)_k, -carbocyclyl(R²⁰)_j, —CH₂carbocyclyl(R²⁰)_j, —(C_1-4 alkylene)_pNR²³R²⁴, -heterocyclyl(R²¹)_h, and —CH₂heterocyclyl(R²¹)_h.

In some embodiments, each R¹² is independently selected from the group consisting of —(C_1-5 alkyl), -phenyl(R¹⁹)_k, —(CH₂)phenyl(R¹⁹)_k, -carbocyclyl(R²⁰)_j, —(CH₂)carbocyclyl(R²⁰)_j, —(CH₂)N(C_1-3 alkyl)₂, and —(CH₂)heterocyclyl(R²¹)_h.

In some embodiments, each R¹³ is independently selected from the group consisting of H and —(C_1-6 alkyl).

In some embodiments, each R¹³ is independently selected from the group consisting of H and —(C_1-3 alkyl).

In some embodiments, each R¹⁴ is independently selected from the group consisting of H, —(C_1-6 alkyl), —CH₂aryl(R¹⁹)_k, and —CH₂carbocyclyl(R²⁰)_j.

In some embodiments, each R¹⁴ is independently selected from the group consisting of H, —(C_1-3 alkyl), —CH₂phenyl(R¹⁹)_k, and —CH₂carbocyclyl(R²⁰)_j.

In some embodiments, each R¹⁵ is independently selected from the group consisting of H and —(C_1-6 alkyl).

In some embodiments, each R¹⁵ is independently selected from the group consisting of H and —(C_1-3 alkyl).

In some embodiments, each R¹⁶ is independently selected from the group consisting of H, —(C_1-6 alkyl), —CH₂aryl(R¹⁹)_k, and —CH₂carbocyclyl(R²⁰)_j.

In some embodiments, each R¹⁶ is independently selected from the group consisting of H, —(C_1-3 alkyl), —CH₂phenyl(R¹⁹)_k, and —CH₂carbocyclyl(R²⁰)_j.

In some embodiments, each R¹⁷ is independently a —(C_1-6 alkyl).

In some embodiments, each R¹⁷ is independently a —(C_1-3 alkyl).

In some embodiments, each R¹⁸ is one substituent attached to the heteroaryl and is independently selected from the group consisting of H, —(C_1-4 alkyl), halide, —CF₃, and —CN.

In some embodiments, each R¹⁸ is one substituent attached to the heteroaryl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN.

In some embodiments, each R¹⁸ is one substituent attached to the heteroaryl and is independently selected from the group consisting of Me, Et, F, Cl, and —CF₃.

In some embodiments, each R¹⁹ is one substituent attached to the aryl and is independently selected from the group consisting of H, —(C_1-4 alkyl), halide, —CF₃, and —CN.

In some embodiments, each R¹⁹ is one substituent attached to the aryl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN.

In some embodiments, each R¹⁹ is one substituent attached to the aryl and is independently selected from the group consisting of Me, Et, F, Cl, and —CF₃.

In some embodiments, each R²⁰ is one substituent attached to the carbocyclyl and is independently selected from the group consisting of H, —(C_1-4 alkyl), halide, —CF₃, and —CN.

In some embodiments, each R²⁰ is one substituent attached to the carbocyclyl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN.

In some embodiments, each R²⁰ is one substituent attached to the carbocyclyl and is independently selected from the group consisting of Me, Et, F, Cl, and —CF₃.

In some embodiments, each R²¹ is one substituent attached to the heterocyclyl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN.

In some embodiments, each R²¹ is one substituent attached to the heterocyclyl and is independently selected from the group consisting of Me, Et, F, Cl, and —CF₃.

In some embodiments, R²² is selected from the group consisting of H, —(C_1-6 alkyl), —(C_1-4 alkylene)_pheterocyclyl(R²¹)_h, —(C_1-4 alkylene)_pcarbocyclyl(R²⁰)_j, —(C_1-4 alkylene)_paryl(R¹⁹)_k, and —(C_1-6 alkylene)_pNR²³R²⁴.

In some embodiments, R²² is selected from the group consisting of H, -Me, -Et, -iPr, -heterocyclyl(R²¹)_h, —(CH₂CH₂)heterocyclyl(R²¹)_h, -carbocyclyl(R²⁰)_j, —(CH₂)phenyl(R¹⁹)_k, and —(CH₂CH₂)N(C_1-3 alkyl)₂.

In some embodiments, each R²³ is independently selected from the group consisting of H and —(C_1-6 alkyl).

In some embodiments, each R²³ is independently selected from the group consisting of Me and Et.

In some embodiments, each R²⁴ is independently selected from the group consisting of H and —(C_1-6 alkyl).

In some embodiments, each R²⁴ is independently selected from the group consisting of Me and Et.

In some embodiments, R²⁵ is selected from the group consisting of H, —(C_1-6 alkyl), —(C_1-4 alkylene)_pheterocyclyl(R²¹)_h, and —(C_1-6 alkylene)_pNR²³R²⁴.

In some embodiments, R²⁵ is selected from the group consisting of H, -Me, -Et, -iPr, —(CH₂CH₂)heterocyclyl(R²¹)_h, and —(CH₂CH₂)N(C_1-3 alkyl)₂.

In some embodiments, each p is independently 0 or 1.

In some embodiments, each q is independently 1 to 4.

In some embodiments, each h is independently 1 to 10.

In some embodiments, each k is independently 1 to 5.

In some embodiments, each j is independently 1 to 12.

In some embodiments, each p is independently 0 or 1; in some embodiments, each p is 0; in some embodiments, each p 1.

In some embodiments, each q is independently 0 to 4; in some embodiments, each q is 0; in some embodiments, each q is 1; in some embodiments, each q is 2; in some embodiments, each q is 3; in some embodiments, each q is 4.

In some embodiments, each h is independently 0 to 10; in some embodiments, each h is 0; in some embodiments, each h is 1; in some embodiments, each h is 2; in some embodiments, each h is 3; in some embodiments, each h is 4.

In some embodiments, each k is independently 0 to 5; in some embodiments, each k is 0; in some embodiments, each k is 1; in some embodiments, each k is 2; in some embodiments, each k is 3.

In some embodiments, each j is independently 0 to 12; in some embodiments, each j is 0; in some embodiments, each j is 1; in some embodiments, each j is 2; in some embodiments, each j is 3; in some embodiments, each j is 4.

In some embodiments, there is the proviso that the compound of Formula I is not a structure selected from the group consisting of:

In some embodiments, each R⁴ is one substituent attached to the heteroaryl ring and is selected from the group consisting of —(C_1-3 alkyl), —CH₂heterocyclyl(R⁹)_h, —NHC(═O)R¹², —NR¹³R¹⁴, and CH₂NR¹⁵R¹⁶.

In some embodiments, at least one R⁹ is halide.

In some embodiments, R¹² is selected from the group consisting of —(C_1-5 alkyl), -phenyl(R¹⁹)_k, —CH₂phenyl(R¹⁹)_k, and -carbocyclyl(R²⁰)_j.

In some embodiments, R¹³ and R¹⁴ are independently selected from H and —(C_1-5 alkyl).

In some embodiments, R¹⁵ and R¹⁶ are independently selected from H and —(C_1-5 alkyl).

In some embodiments, k is 1 or 2 and each R⁸ is independently a halide.

In some embodiments, k is 2, one R⁸ is halide and the other R⁸ is —CH₂NHSO₂R¹⁷.

In some embodiments, R¹⁷ is —(C_1-3 alkyl).

In some embodiments, k is 2, one R⁸ is halide and the other R⁸ is —NHCH₂CH₂NR¹³R¹⁴.

In some embodiments, R¹³ and R¹⁴ are independently selected from H and —(C_1-3 alkyl).

In some embodiments, R³ is selected from the group consisting of -pyridinyl(R⁶)_q, -imidazolyl(R⁶)_q, -furanyl(R⁶)_q, and -thiophenyl(R⁶)_q.

In some embodiments, q is 0 or 1, R⁶ is selected from the group consisting of halide, —(C_1-3 alkyl), and —C(═O)R¹⁷, wherein R¹⁷ is —(C_1-2 alkyl).

In some embodiments, R³ is selected from the group consisting of -piperidinyl(R⁷)_h and -piperazinyl(R⁷)_h.

In some embodiments, q is 1, and R⁷ is selected from the group consisting of H and —(C_1-3 alkyl).

In some embodiments, R² is H; in other embodiments, R² is halide, e.g. F.

In some embodiments, R¹ is -heteroaryl(R⁴)_q.

In some embodiments, R¹ is -pyridinyl(R⁴)_q.

In some embodiments, R¹ is -pyridin-3-yl(R⁴)_q.

In some embodiments, R¹ is -pyrimidinyl(R⁴)_q.

In some embodiments, R¹ is -pyrimidin-5-yl(R⁴)_q.

In some embodiments, R¹ is -pyrimidin-5-yl(R⁴)_q and q is 0.

In some embodiments, R¹ is -pyrazinyl(R⁴)_q.

In some embodiments, R¹ is -pyrazolyl(R⁴)_q.

In some embodiments, R¹ is -pyrazol-4-yl(R⁴)_q, q is 1, and R⁴ is Me.

In some embodiments, R¹ is -pyrazol-4-yl(R⁴)_q and q is 0.

In some embodiments, R¹ is -imidazolyl(R⁴)_q.

In some embodiments, R¹ is -imidazol-5-yl(R⁴)_q, q is 1, and R⁴ is Me.

In some embodiments, R¹ is -imidazol-5-yl(R⁴)_q, q is 2, and both R⁴ are Me.

In some embodiments, R¹ is -heterocyclyl(R⁵)_h.

In some embodiments, R¹ is -piperidinyl(R⁵)_h.

In some embodiments, R¹ is -piperidin-4-yl(R⁵)_h.

In some embodiments, R¹ is -piperidin-4-yl(R⁵)_h, and h is 0.

In some embodiments, R¹ is -tetrahydropyridinyl(R⁵)_h.

In some embodiments, R¹ is -1,2,3,6-tetrahydropyridinyl(R⁵)_h.

In some embodiments, R¹ is -1,2,3,6-tetrahydropyridinyl(R⁵)_h, and h is 0.

In some embodiments, R³ is H.

In some embodiments, R³ is -heteroaryl(R⁶)_q.

In some embodiments, R³ is -heterocyclyl(R⁷)_h.

In some embodiments, R³ is -piperidinyl(R⁷)_h.

In some embodiments, R³ is -piperazinyl(R⁷)_h.

In some embodiments, R³ is -aryl(R⁸)_k.

In some embodiments, R³ is -pyridinyl(R⁶)_q.

In some embodiments, R³ is -pyridin-3-yl(R⁶)_q.

In some embodiments, R³ is -pyridin-4-yl(R⁶)_q.

In some embodiments, R³ is -pyridin-5-yl(R⁶)_q.

In some embodiments, R³ is -pyridin-3-yl(R⁶)_q, q is 0.

In some embodiments, R³ is -pyridin-4-yl(R⁶)_q, q is 0.

In some embodiments, R³ is -pyridin-5-yl(R⁶)_q, q is 0.

In some embodiments, R³ is -imidazolyl(R⁶)_q.

In some embodiments, R³ is -imidazol-1-yl(R⁶)_q, q is 1, and R⁶ is —(C_1-3 alkyl).

In some embodiments, R³ is -imidazol-1-yl(R⁶)_q, q is 1, and R⁶ is methyl.

In some embodiments, R³ is -furanyl(R⁶)_q.

In some embodiments, R³ is -furan-2-yl(R⁶)_q.

In some embodiments, R³ is -furan-2-yl(R⁶)_q and q is 0.

In some embodiments, R³ is -furan-3-yl(R⁶)_q.

In some embodiments, R³ is -furan-3-yl(R⁶)_q and q is 0.

In some embodiments, R³ is -thiophenyl(R⁶)_q.

In some embodiments, R³ is -thiophen-2-yl(R⁶)_q.

In some embodiments, R³ is -thiophen-2-yl(R⁶)_q and q is 0.

In some embodiments, R³ is -thiophen-2-yl(R⁶)_q, q is 1 or 2, and each R⁶ is independently a halide.

In some embodiments, R³ is -thiophen-2-yl(R⁶)_q, q is 1 or 2, and R⁶ is F.

In some embodiments, R³ is -thiophen-2-yl(R⁶)_q, q is 1 or 2, and each R⁶ is independently —(C_1-6 alkyl).

In some embodiments, R³ is -thiophen-2-yl(R⁶)_q, q is 1 or 2, and each R⁶ is independently —(C_1-2 alkyl).

In some embodiments, R³ is -thiophen-2-yl(R⁶)_q, q is 1 or 2, and R⁶ is methyl.

In some embodiments, R³ is -thiophen-2-yl(R⁶)_q, q is 1 or 2, and R⁶ is —CF₃.

In some embodiments, R³ is -thiophen-2-yl(R⁶)_q, q is 1 or 2, and R⁶ is CN.

In some embodiments, R³ is -thiophen-2-yl(R⁶)_q, q is 1, and R⁶ is —C(═O)R¹⁷.

In some embodiments, R³ is -thiophen-2-yl(R⁶)_q, q is 1, R⁶ is —C(═O)R¹⁷, and R¹⁷ is —(C_1-6 alkyl).

In some embodiments, R³ is -thiophen-2-yl(R⁶)_q, q is 1, R⁶ is —C(═O)R¹⁷, and R¹⁷ is —(C_1-4 alkyl).

In some embodiments, R³ is -thiophen-2-yl(R⁶)_q, q is 1, R⁶ is —C(═O)R¹⁷, and R¹⁷ is —(C_1-2 alkyl).

In some embodiments, R³ is -thiophen-2-yl(R⁶)_q, q is 1, R⁶ is —C(═O)R¹⁷, and R¹⁷ is methyl.

In some embodiments, R³ is -thiophen-3-yl(R⁶)_q.

In some embodiments, R³ is -thiophen-3-yl(R⁶)_q and q is 0.

In some embodiments, R³ is -thiophen-3-yl(R⁶)_q, q is 1 or 2, and each R⁶ is independently halide.

In some embodiments, R³ is -thiophen-3-yl(R⁶)_q, q is 1 or 2, and R⁶ is F.

In some embodiments, R³ is -thiophen-3-yl(R⁶)_q, q is 1 or 2, and each R⁶ is independently —(C_1-6 alkyl).

In some embodiments, R³ is -thiophen-3-yl(R⁶)_q, q is 1 or 2, and each R⁶ is independently —(C_1-2 alkyl).

In some embodiments, R³ is -thiophen-3-yl(R⁶)_q, q is 1 or 2, and R⁶ is methyl.

In some embodiments, R³ is -thiophen-3-yl(R⁶)_q, q is 1 or 2, and R⁶ is —CF₃.

In some embodiments, R³ is -thiophen-3-yl(R⁶)_q, q is 1 or 2, and R⁶ is CN.

In some embodiments, R³ is -thiophen-3-yl(R⁶)_q, q is 1, and R⁶ is —C(═O)R¹⁷.

In some embodiments, R³ is -thiophen-3-yl(R⁶)_q, q is 1, R⁶ is —C(═O)R¹⁷, and R¹⁷ is —(C_1-4 alkyl).

In some embodiments, R³ is -thiophen-3-yl(R⁶)_q, q is 1, R⁶ is —C(═O)R¹⁷, and R¹⁷ is —(C_1-2 alkyl).

In some embodiments, R³ is -thiophen-3-yl(R⁶)_q, q is 1, R⁶ is —C(═O)R¹⁷, and R¹⁷ is methyl.

In some embodiments, R³ is selected from the group consisting of:

In some embodiments, R³ is -phenyl(R⁸)_k.

In some embodiments, R³ is -phenyl(R⁸)_k and k is 0.

In some embodiments, R³ is -phenyl(R⁸)_k, k is 1 or 2, and each R⁸ is independently a halide.

In some embodiments, R³ is -phenyl(R⁸)_k, k is 1 or 2, and R⁸ is F.

In some embodiments, R³ is -phenyl(R⁸)_k, k is 1, and R⁸ is F.

In some embodiments, R³ is -phenyl(R⁸)_k, k is 2, one R⁸ is a halide and the other R⁸ is —(C_1-6 alkylene)_pNHSO₂R¹⁷.

In some embodiments, R³ is -phenyl(R⁸)_k, k is 2, one R⁸ is a halide and the other R⁸ is —(C_1-4 alkylene)_pNHSO₂R¹⁷, and p is 1.

In some embodiments, R³ is -phenyl(R⁸)_k, k is 2, one R⁸ is a halide and the other R⁸ is —(C_1-2 alkylene)_pNHSO₂R¹⁷, and p is 1.

In some embodiments, R³ is -phenyl(R⁸)_k, k is 2, one R⁸ is a halide and the other R⁸ is —CH₂NHSO₂R¹⁷.

In some embodiments, R³ is -phenyl(R⁸)_k, k is 2, one R⁸ is a halide and the other R⁸ is —CH₂NHSO₂R¹⁷, and R¹⁷ is —(C_1-4 alkyl).

In some embodiments, R³ is -phenyl(R⁸)_k, k is 2, one R⁸ is a halide and the other R⁸ is —CH₂NHSO₂R¹⁷, and R¹⁷ is —(C_1-2 alkyl).

In some embodiments, R³ is -phenyl(R⁸)_k, k is 2, one R⁸ is a halide and the other R⁸ is —CH₂NHSO₂R¹⁷, and R¹⁷ is methyl.

In some embodiments, R³ is -phenyl(R⁸)_k, k is 2, one R⁸ is F and the other R⁸ is —CH₂NHSO₂R¹⁷, and R¹⁷ is —(C_1-2 alkyl).

In some embodiments, R³ is -phenyl(R⁸)_k, k is 2, one R⁸ is F and the other R⁸ is —CH₂NHSO₂R¹⁷, and R¹⁷ is methyl.

In some embodiments, R³ is -phenyl(R⁸)_k, k is 2, one R⁸ is halide and the other R⁸ is —NR¹³(C_1-6 alkylene)NR¹³R¹⁴.

In some embodiments, R³ is -phenyl(R⁸)_k, k is 2, one R⁸ is halide and the other R⁸ is —NR¹³(C_1-5 alkylene)NR¹³R¹⁴.

In some embodiments, R³ is -phenyl(R⁸)_k, k is 2, one R⁸ is halide and the other R⁸ is —NR¹³(C_1-4 alkylene)NR¹³R¹⁴.

In some embodiments, R³ is -phenyl(R⁸)_k, k is 2, one R⁸ is halide and the other R⁸ is —NR¹³(C_1-3 alkylene)NR¹³R¹⁴.

In some embodiments, R³ is -phenyl(R⁸)_k, k is 2, one R⁸ is halide and the other R⁸ is —NR¹³CH₂CH₂NR¹³R¹⁴.

In some embodiments, R³ is -phenyl(R⁸)_k, k is 2, R⁸ is halide and the other R⁸ is —NHCH₂CH₂NR¹³R¹⁴, and R¹³ and R¹⁴ are independently selected from —(C_1-6 alkyl).

In some embodiments, R³ is -phenyl(R⁸)_k, k is 2, one R⁸ is halide and the other R⁸ is —NHCH₂CH₂NR¹³R¹⁴, and R¹³ and R¹⁴ are independently selected from —(C_1-4 alkyl).

In some embodiments, R³ is -phenyl(R⁸)_k, k is 2, one R⁸ is halide and the other R⁸ is —NHCH₂CH₂NR¹³R¹⁴, and R¹³ and R¹⁴ are independently selected from —(C_1-2 alkyl).

In some embodiments, R³ is -phenyl(R⁸)_k, k is 2, one R⁸ is halide and the other R⁸ is —NHCH₂CH₂NR¹³R¹⁴, and R¹³ and R¹⁴ are both methyl.

In some embodiments, R³ is -phenyl(R⁸)_k, k is 2, one R⁸ is F and the other R⁸ is —NHCH₂CH₂NR¹³R¹⁴, and R¹³ and R¹⁴ are independently selected from —(C_1-2 alkyl).

In some embodiments, R³ is -phenyl(R⁸)_k, k is 2, one R⁸ is F and the other R⁸ is —NHCH₂CH₂NR¹³R¹⁴, and R¹³ and R¹⁴ are both methyl.

In some embodiments, R³ is -phenyl(R⁸)_k, k is 2, one R⁸ is halide and the other R⁸ is —OCH₂CH₂NR²³R²⁴.

In some embodiments, R³ is -phenyl(R⁸)_k, k is 2, one R⁸ is halide and the other R⁸ is —OCH₂CH₂NR²³R²⁴, and R²³ and R²⁴ are independently a —(C_1-2 alkyl).

In some embodiments, R³ is -phenyl(R⁸)_k, k is 2, one R⁸ is halide and the other R⁸ is —OCH₂CH₂NR²³R²⁴, and R²³ and R²⁴ are both methyl.

In some embodiments, R³ is -phenyl(R⁸)_k, k is 2, one R⁸ is F and the other R⁸ is —OCH₂CH₂NR²³R²⁴, and R²³ and R²⁴ are both methyl.

In some embodiments, R³ is -phenyl(R⁸)_k, k is 2, one R⁸ is halide and the other R⁸ is —CH₂NHSO₂R¹⁷, and R¹⁷ is —(C_1-4 alkyl).

In some embodiments, R³ is -phenyl(R⁸)_k, k is 2, one R⁸ is halide and the other R⁸ is —CH₂NHSO₂R¹⁷, and R¹⁷ is —(C_1-2 alkyl).

In some embodiments, R³ is -phenyl(R⁸)_k, k is 2, one R⁸ is halide and the other R⁸ is —CH₂NHSO₂R¹⁷, and R¹⁷ is methyl.

In some embodiments, R³ is -phenyl(R⁸)_k, k is 2, one R⁸ is F and the other R⁸ is —CH₂NHSO₂R¹⁷, and R¹⁷ is —(C_1-2 alkyl).

In some embodiments, R³ is -phenyl(R⁸)_k, k is 2, one R⁸ is F and the other R⁸ is —CH₂NHSO₂R¹⁷, and R¹⁷ is methyl.

In some embodiments, R³ is selected from the group consisting of:

In some embodiments, R³ is -piperidinyl(R⁷)_h.

In some embodiments, R³ is -piperidin-1-yl(R⁷)_h.

In some embodiments, R³ is -piperidin-1-yl(R⁷)_h and h is 0.

In some embodiments, R³ is -piperidin-1-yl(R⁷)_h, h is 1 or 2, and each R⁷ is independently selected from a halide.

In some embodiments, R³ is -piperazinyl(R⁷)_h.

In some embodiments, R³ is -piperazin-1-yl(R⁷)_h.

In some embodiments, R³ is -piperazin-1-yl(R⁷)_h, h is 1, and R⁷ is C_1-3 alkyl.

In some embodiments, R³ is -piperazin-1-yl(R⁷)_h, h is 1, and R⁷ is methyl.

In some embodiments, R³ is -morpholinyl(R⁷)_h.

In some embodiments, R³ is -morpholin-1-yl(R⁷)_h.

In some embodiments, R³ is -morpholin-1-yl(R⁷)_h and h is 0.

In some embodiments, R³ is selected from the group consisting of:

In some embodiments, q is 0.

In some embodiments, at least one R⁴ is a halide.

In some embodiments, at least one R⁴ is a F.

In some embodiments, R⁴ is F.

In some embodiments, at least one R⁴ is —(C_1-6 alkyl).

In some embodiments, at least one R⁴ is —(C_1-5 alkyl).

In some embodiments, at least one R⁴ is —(C_1-4 alkyl).

In some embodiments, at least one R⁴ is —(C_1-3 alkyl).

In some embodiments, at least one R⁴ is —(C_1-2 alkyl).

In some embodiments, R⁴ is a methyl.

In some embodiments, at least one R⁴ is —(C_1-4 alkylene)_pheterocyclyl(R⁹)_h and p is 0 or 1.

In some embodiments, at least one R⁴ is —(C_1-3 alkylene)_pheterocyclyl(R⁹)_h and p is 0 or 1.

In some embodiments, at least one R⁴ is —(C_1-2 alkylene)_pheterocyclyl(R⁹)_h and p is 0 or 1.

In some embodiments, at least one R⁴ is —CH₂pyrrolidinyl(R⁹)_h.

In some embodiments, at least one R⁴ is —CH₂pyrrolidinyl(R⁹)_h and h is 0.

In some embodiments, R⁴ is a —CH₂pyrrolidinyl(R⁹)_h and h is 0.

In some embodiments, at least one R⁴ is —CH₂pyrrolidinyl(R⁹)_h, h is 1 or 2, and at least one R⁹ is halide.

In some embodiments, at least one R⁴ is —CH₂pyrrolidinyl(R⁹)_h, h is 1 or 2, and at least one R⁹ is F.

In some embodiments, R⁴ is a —CH₂pyrrolidinyl(R⁹)_h, h is 1 or 2, and at least one R⁹ is halide.

In some embodiments, R⁴ is —CH₂pyrrolidinyl(R⁹)_h, h is 1 or 2, and at least one R⁹ is F.

In some embodiments, R⁴ is a —CH₂pyrrolidinyl(R⁹)_h, h is 1 or 2, and each R⁹ is F.

In some embodiments, at least one R⁴ is —CH₂piperidinyl(R⁹)_h.

In some embodiments, at least one R⁴ is —CH₂piperidinyl(R⁹)_h and h is 0.

In some embodiments, R⁴ is a —CH₂piperidinyl(R⁹)_h and h is 0.

In some embodiments, at least one R⁴ is —CH₂piperidinyl(R⁹)_h and at least one R⁹ is halide.

In some embodiments, at least one R⁴ is —CH₂piperidinyl(R⁹)_h and at least one R⁹ is F.

In some embodiments, at least one R⁴ is —CH₂piperidinyl(R⁹)_h, h is 1 or 2, and at least one R⁹ is halide.

In some embodiments, at least one R⁴ is —CH₂piperidinyl(R⁹)_h, h is 1 or 2, and at least one R⁹ is F.

In some embodiments, R⁴ is —CH₂piperidinyl(R⁹)_h, h is 1 or 2, and each R⁹ is a halide.

In some embodiments, R⁴ is —CH₂piperidinyl(R⁹)_h, h is 1 or 2, and each R⁹ is F.

In some embodiments, R⁴ is a —CH₂piperidinyl(R⁹)_h, h is 1 or 2, and each R⁹ is F.

In some embodiments, R⁴ is a

In some embodiments, at least one R⁴ is —(C_1-4 alkylene)_pcarbocyclyl(R¹⁰)_j.

In some embodiments, at least one R⁴ is —(C_1-4 alkylene)_pcarbocyclyl(R¹⁰)_j and j is 0 or 1.

In some embodiments, at least one R⁴ is —(C_1-3 alkylene)_pcarbocyclyl(R¹⁰)_j and j is 0 or 1.

In some embodiments, at least one R⁴ is —(C_1-2 alkylene)_pcarbocyclyl(R¹⁰)_j and j is 0 or 1.

In some embodiments, at least one R⁴ is —CH₂carbocyclyl(R¹⁰)_j.

In some embodiments, R⁴ is a —CH₂carbocyclyl(R¹⁰)_j.

In some embodiments, at least one R⁴ is —(C_1-4 alkylene)_paryl(R¹¹)_k and k is 0 or 1.

In some embodiments, at least one R⁴ is —(C_1-3 alkylene)_paryl(R¹¹)_k and k is 0 or 1.

In some embodiments, at least one R⁴ is —(C_1-2 alkylene)_paryl(R¹¹)_k and k is 0 or 1.

In some embodiments, at least one R⁴ is —CH₂aryl(R¹¹)_k.

In some embodiments, at least one R⁴ is —CH₂phenyl(R¹¹)_k.

In some embodiments, R⁴ is a —CH₂phenyl(R¹¹)_k.

In some embodiments, at least one R⁴ is —NHC(═O)R¹².

In some embodiments, R⁴ is a —NHC(═O)R¹².

In some embodiments, at least one R⁴ is —NHC(═O)R¹² and R¹² is —(C_1-9 alkyl).

In some embodiments, at least one R⁴ is —NHC(═O)R¹² and R¹² is —(C_1-8 alkyl).

In some embodiments, at least one R⁴ is —NHC(═O)R¹² and R¹² is —(C_1-7 alkyl).

In some embodiments, at least one R⁴ is —NHC(═O)R¹² and R¹² is —(C_1-6 alkyl).

In some embodiments, at least one R⁴ is —NHC(═O)R¹² and R¹² is —(C_1-5 alkyl).

In some embodiments, R⁴ is a —NHC(═O)R¹² and R¹² is —(C_1-5 alkyl).

In some embodiments, at least one R⁴ is —NHC(═O)R¹² and R¹² is —(C_1-4 alkyl).

In some embodiments, R⁴ is a —NHC(═O)R¹² and R¹² is —(C_1-4 alkyl).

In some embodiments, at least one R⁴ is —NHC(═O)R¹² and R¹² is —(C_1-3 alkyl).

In some embodiments, R⁴ is a —NHC(═O)R¹² and R¹² is —(C_1-3 alkyl).

In some embodiments, at least one R⁴ is —NHC(═O)R¹² and R¹² is —(C_1-2 alkyl).

In some embodiments, at least one R⁴ is —NHC(═O)R¹² and R¹² is —(C_2-5 alkyl).

In some embodiments, R⁴ is a —NHC(═O)R¹² and R¹² is —(C_2-5 alkyl).

In some embodiments, at least one R⁴ is —NHC(═O)R¹² and R¹² is —(C_3-4 alkyl).

In some embodiments, at least one R⁴ is —NHC(═O)R¹² and R¹² is -aryl(R¹⁹)_k.

In some embodiments, at least one R⁴ is —NHC(═O)R¹², R¹² is -phenyl(R¹⁹)_k, and k is 0.

In some embodiments, R⁴ is a —NHC(═O)R¹², R¹² is -phenyl(R¹⁹)_k, and k is 0.

In some embodiments, at least one R⁴ is —NHC(═O)R¹² and R¹² is —CH₂aryl(R¹⁹)_k.

In some embodiments, at least one R⁴ is —NHC(═O)R¹², R¹² is —CH₂phenyl(R¹⁹)_k, and k is 0.

In some embodiments, R⁴ is —NHC(═O)R¹², R¹² is —CH₂phenyl(R¹⁹)_k, and k is 0.

In some embodiments, at least one R⁴ is —NHC(═O)R¹² and R¹² is -heteroaryl(R¹⁸)_q.

In some embodiments, at least one R⁴ is —NHC(═O)R¹² and R¹² is -carbocyclyl(R²⁰)_j.

In some embodiments, at least one R⁴ is —NHC(═O)R¹², R¹² is -carbocyclyl(R²⁰)_j, and j is 0.

In some embodiments, at least one R⁴ is —NHC(═O)R¹², R¹² is -cyclopropyl(R²⁰)_j, and j is 0.

In some embodiments, R⁴ is a —NHC(═O)R¹², R¹² is -cyclopropyl(R²⁰)_j, and j is 0.

In some embodiments, at least one R⁴ is —NHC(═O)R¹², R¹² is -cyclobutyl(R²⁰)_j, and j is 0.

In some embodiments, R⁴ is a —NHC(═O)R¹², R¹² is -cyclobutyl(R²⁰)_j, and j is 0.

In some embodiments, at least one R⁴ is —NHC(═O)R¹², R¹² is -cyclopentyl(R²⁰)_j, and j is 0.

In some embodiments, R⁴ is a —NHC(═O)R¹², R¹² is -cyclopentyl(R²⁰)_j, and j is 0.

In some embodiments, at least one R⁴ is —NHC(═O)R¹², R¹² is -cyclohexyl(R²⁰)_j, and j is 0.

In some embodiments, R⁴ is a —NHC(═O)R¹², R¹² is -cyclohexyl(R²⁰)_j, and j is 0.

In some embodiments, at least one R⁴ is —NHC(═O)R¹², R¹² is —CH₂carbocyclyl(R²⁰)_j, and j is 0.

In some embodiments, at least one R⁴ is —NHC(═O)R¹², R¹² is —CH₂cyclopropyl(R²⁰)_j, and j is 0.

In some embodiments, at least one R⁴ is —NR¹³R¹⁴.

In some embodiments, at least one R⁴ is —NR¹³R¹⁴, and R¹³ and R¹⁴ are independently selected from the group consisting of H and —(C_1-6 alkyl).

In some embodiments, at least one R⁴ is —NR¹³R¹⁴, and R¹³ and R¹⁴ are independently selected from the group consisting of H and —(C_1-5 alkyl).

In some embodiments, at least one R⁴ is —NR¹³R¹⁴, and R¹³ and R¹⁴ are independently selected from the group consisting of H and —(C_1-4 alkyl).

In some embodiments, at least one R⁴ is —NR¹³R¹⁴, and R¹³ and R¹⁴ are independently selected from the group consisting of H and —(C_1-3 alkyl).

In some embodiments, at least one R⁴ is —NR¹³R¹⁴, and R¹³ and R¹⁴ are independently selected from the group consisting of H and —(C_1-2 alkyl).

In some embodiments, at least one R⁴ is —NR¹³R¹⁴, and R¹³ and R¹⁴ are independently selected from the group consisting of H and methyl.

In some embodiments, at least one R⁴ is —NH₂.

In some embodiments, R⁴ is a —NH₂.

In some embodiments, at least one R⁴ is —NHR¹⁴ and R¹⁴ is —(C_1-4 alkyl).

In some embodiments, at least one R⁴ is —NHR¹⁴ and R¹⁴ is —(C_1-3 alkyl).

In some embodiments, at least one R⁴ is —NHR¹⁴ and R¹⁴ is —(C_1-2 alkyl).

In some embodiments, R⁴ is a —NHR¹⁴ and R¹⁴ is —(C_1-2 alkyl).

In some embodiments, at least one R⁴ is —NHR¹⁴ and R¹⁴ is —CH₂aryl(R¹⁹)_k.

In some embodiments, at least one R⁴ is —NHR⁴, R¹⁴ is —CH₂phenyl(R¹⁹)_k, and k is 0.

In some embodiments, R⁴ is —NHR⁴, R¹⁴ is —CH₂phenyl(R¹⁹)_k, and k is 0.

In some embodiments, at least one R⁴ is —NHR¹⁴ and R¹⁴ is —CH₂carbocyclyl(R²⁰)_j.

In some embodiments, at least one R⁴ is —NHR¹⁴, R¹⁴ is —CH₂cyclopropyl(R²⁰)_j, and j is 0.

In some embodiments, R⁴ is a —NHR⁴, R¹⁴ is —CH₂cyclopropyl(R²⁰)_j, and j is 0.

In some embodiments, at least one R⁴ is —NHR⁴, R¹⁴ is —CH₂cyclobutyl(R²⁰)_j, and j is 0.

In some embodiments, R⁴ is a —NHR⁴, R¹⁴ is —CH₂cyclobutyl(R²⁰)_j, and j is 0.

In some embodiments, at least one R⁴ is —NHR⁴, R¹⁴ is —CH₂cyclopentyl(R²⁰)_j, and j is 0.

In some embodiments, R⁴ is a —NHR¹⁴, R¹⁴ is —CH₂cyclopentyl(R²⁰)_j, and j is 0.

In some embodiments, at least one R⁴ is —NHR¹⁴, R¹⁴ is —CH₂cyclohexyl(R²⁰)_j, and j is 0.

In some embodiments, R⁴ is a —NHR¹⁴, R¹⁴ is —CH₂cyclohexyl(R²⁰)_j, and j is 0.

In some embodiments, at least one R⁴ is —(C_1-6 alkylene)NR¹⁵R¹⁶.

In some embodiments, at least one R⁴ is —(C_1-5 alkylene)NR¹⁵R¹⁶.

In some embodiments, at least one R⁴ is —(C_1-4 alkylene)NR¹⁵R¹⁶.

In some embodiments, at least one R⁴ is —(C_1-3 alkylene)NR¹⁵R¹⁶.

In some embodiments, at least one R⁴ is —(C_1-2 alkylene)NR¹⁵R¹⁶.

In some embodiments, at least one R⁴ is —CH₂NR¹⁵R¹⁶.

In some embodiments, R⁴ is a —CH₂NR¹⁵R¹⁶.

In some embodiments, at least one R⁴ is —CH₂NR¹⁵R¹⁶, and R¹⁵ and R¹⁶ are independently selected from the group consisting of H and —(C_1-6 alkyl).

In some embodiments, at least one R⁴ is —CH₂NR¹⁵R¹⁶, and R¹⁵ and R¹⁶ are independently selected from the group consisting of H and —(C_1-5 alkyl).

In some embodiments, at least one R⁴ is —CH₂NR¹⁵R¹⁶, and R¹⁵ and R¹⁶ are independently selected from the group consisting of H and —(C_1-4 alkyl).

In some embodiments, at least one R⁴ is —CH₂NR¹⁵R¹⁶, and R¹⁵ and R¹⁶ are independently selected from the group consisting of H and —(C_1-3 alkyl).

In some embodiments, at least one R⁴ is —CH₂NR¹⁵R¹⁶, and R¹⁵ and R¹⁶ are independently selected from the group consisting of H and —(C_1-2 alkyl).

In some embodiments, at least one R⁴ is —CH₂NR¹⁵R¹⁶, and R¹⁵ and R¹⁶ are independently selected from the group consisting of H and methyl.

In some embodiments, R⁴ is a —CH₂NR¹⁵R¹⁶, and R¹⁵ and R¹⁶ are independently selected from the group consisting of H and methyl.

In some embodiments, at least one R⁴ is —CH₂NH₂.

In some embodiments, R⁴ is a —CH₂NH₂.

In some embodiments, at least one R⁴ is —CH₂NMe₂.

In some embodiments, R⁴ is —CH₂NMe₂.

In some embodiments, at least one R⁴ is —CH₂NHR¹⁶ and R¹⁶ is —(C_1-4 alkyl).

In some embodiments, at least one R⁴ is —CH₂NHR¹⁶ and R¹⁶ is —(C_1-3 alkyl).

In some embodiments, at least one R⁴ is —CH₂NHR¹⁶ and R¹⁶ is —(C_1-2 alkyl).

In some embodiments, R⁴ is a —CH₂NHR¹⁶ and R¹⁶ is —(C_1-2 alkyl).

In some embodiments, at least one R⁴ is —CH₂NHR¹⁶ and R¹⁶ is —CH₂aryl(R¹⁹)_k.

In some embodiments, at least one R⁴ is —CH₂NHR¹⁶, R¹⁶ is —CH₂phenyl(R¹⁹)_k, and k is 0.

In some embodiments, R⁴ is a —CH₂NHR¹⁶, R¹⁶ is —CH₂phenyl(R¹⁹)_k, and k is 0.

In some embodiments, at least one R⁴ is —CH₂NHR¹⁶ and R¹⁶ is —CH₂carbocyclyl(R²⁰)_j.

In some embodiments, at least one R⁴ is —CH₂NHR¹⁶, R¹⁶ is —CH₂cyclopropyl(R²⁰)_j, and j is 0.

In some embodiments, R⁴ is a —CH₂NHR¹⁶, R¹⁶ is —CH₂cyclopropyl(R²⁰)_j, and j is 0.

In some embodiments, at least one R⁴ is —CH₂NHR¹⁶, R¹⁶ is —CH₂cyclobutyl(R²⁰)_j, and j is 0.

In some embodiments, R⁴ is a —CH₂NHR¹⁶, R¹⁶ is —CH₂cyclobutyl(R²⁰)_j, and j is 0.

In some embodiments, at least one R⁴ is —CH₂NHR¹⁶, R¹⁶ is —CH₂cyclopentyl(R²⁰)_j, and j is 0.

In some embodiments, R⁴ is a —CH₂NHR¹⁶, R¹⁶ is —CH₂cyclopentyl(R²⁰)_j, and j is 0.

In some embodiments, at least one R⁴ is —CH₂NHR¹⁶, R¹⁶ is —CH₂cyclohexyl(R²⁰)_j, and j is 0.

In some embodiments, R⁴ is a —CH₂NHR¹⁶, R¹⁶ is —CH₂cyclohexyl(R²⁰)_j, and j is 0.

In some embodiments, at least one R⁴ is —OR²².

In some embodiments, at least one R⁴ is —OH.

In some embodiments, R⁴ is a —OH.

In some embodiments, at least one R⁴ is —OR²² and R²² is —(C_1-3 alkyl).

In some embodiments, at least one R⁴ is —OR²² and R²² is —(C_1-2 alkyl).

In some embodiments, at least one R⁴ is —OMe.

In some embodiments, R⁴ is a —OMe.

In some embodiments, at least one R⁴ is —OR²², R²² is -heterocyclyl(R²¹)_h, and his 0.

In some embodiments, R⁴ is a —OR²², R²² is -heterocyclyl(R²¹)_h, and h is 0.

In some embodiments, at least one R⁴ is —OR²², R²² is -carbocyclyl(R²⁰)_j, and j is 0.

In some embodiments, R⁴ is a —OR²², R²² is -carbocyclyl(R²⁰)_j, and j is 0.

In some embodiments, at least one R⁴ is —OR²², R²² is —(C_1-4 alkylene)heterocyclyl(R²¹)_h, and h is 0.

In some embodiments, at least one R⁴ is —OR²², R²² is —(CH₂CH₂)heterocyclyl(R²¹)_h, and h is 0.

In some embodiments, R⁴ is a —OR²², R²² is —(CH₂CH₂)heterocyclyl(R²¹)_h, and his 0.

In some embodiments, at least one R⁴ is —OR²², R²² is —(C_1-4 alkylene)NR²³R²⁴ and R²³ and R²⁴ are independently a —(C_1-4 alkyl).

In some embodiments, at least one R⁴ is —OR²², R²² is —(CH₂CH₂)NR²³R²⁴ and R²³ and R²⁴ are independently a —(C_1-2 alkyl).

In some embodiments, at least one R⁴ is —OR²², and R²² is —(CH₂CH₂)NMe₂.

In some embodiments, R⁴ is a —OR²², and R²² is —(CH₂CH₂)NMe₂.

In some embodiments, at least one R⁴ is —OR²², R²² is —(C_1-4 alkylene)aryl(R¹⁹)_k, k is 0 or 1 and R¹⁹ is halide.

In some embodiments, at least one R⁴ is —OR²², R²² is —(CH₂CH₂)phenyl(R¹⁹)_k, k is 0 or 1 and R¹⁹ is a halide.

In some embodiments, R⁴ is a —OR²², R²² is —(CH₂CH₂)phenyl(R¹⁹)_k, k is 0 or 1 and R¹⁹ is a halide.

In some embodiments, at least one R⁴ is —OR²², R²² is —(CH₂)phenyl(R¹⁹)_k, k is 0 or 1 and R¹⁹ is a halide.

In some embodiments, R⁴ is a —OR²², R²² is —(CH₂)phenyl(R¹⁹)_k, k is 0 or 1 and R¹⁹ is a halide.

In some embodiments, h is 0.

In some embodiments, at least one R⁵ is a halide.

In some embodiments, at least one R⁵ is a F.

In some embodiments, at least one R⁵ is —(C_1-6 alkyl).

In some embodiments, at least one R⁵ is —(C_1-5 alkyl).

In some embodiments, at least one R⁵ is —(C_1-4 alkyl).

In some embodiments, at least one R⁵ is —(C_1-3 alkyl).

In some embodiments, at least one R⁵ is —(C_1-2 alkyl).

In some embodiments, at least one R⁵ is methyl.

In some embodiments, at least one R⁶ is a halide.

In some embodiments, at least one R⁶ is a F.

In some embodiments, at least one R⁶ is —(C_1-4 alkyl).

In some embodiments, at least one R⁶ is —(C_1-3 alkyl).

In some embodiments, at least one R⁶ is —(C_1-2 alkyl).

In some embodiments, at least one R⁶ is methyl.

In some embodiments, R⁶ is a methyl.

In some embodiments, at least one R⁶ is —C(═O)(C_1-3 alkyl).

In some embodiments, at least one R⁶ is —C(═O)Me.

In some embodiments, R⁶ is a —C(═O)Me.

In some embodiments, at least one R⁸ is F.

In some embodiments, at least one R⁸ is Cl.

In some embodiments, at least one R⁸ is Br.

In some embodiments, at least one R⁸ is I.

In some embodiments, at least one R¹² is —(C_1-9 alkyl).

In some embodiments, at least one R¹² is methyl.

In some embodiments, at least one R¹² is ethyl.

In some embodiments, at least one R¹² is propyl.

In some embodiments, at least one R¹² is —(C_1-2 alkyl).

In some embodiments, at least one R¹² is —(C_1-3 alkyl).

In some embodiments, at least one R¹² is —(C_1-4 alkyl).

In some embodiments, at least one R¹² is —(C_1-5 alkyl).

In some embodiments, at least one R¹² is —(C_2-9 alkyl).

In some embodiments, at least one R¹² is —(C_3-9 alkyl).

In some embodiments, at least one R¹² is —(C_4-9 alkyl).

In some embodiments, at least one R¹² is —(C_5-9 alkyl).

In some embodiments, at least one R¹² is —(C_6-9 alkyl).

In some embodiments, at least one R¹² is —(C_7-9 alkyl).

In some embodiments, at least one R¹² is —(C_8-9 alkyl).

In some embodiments, at least one R¹² is -carbocyclyl(R²⁰)_j.

In some embodiments, at least one R¹² is cyclopropyl.

In some embodiments, at least one R¹² is cyclobutyl.

In some embodiments, at least one R¹² is cyclopentyl.

In some embodiments, at least one R¹² is cyclohexyl.

In some embodiments, at least one R¹² is —(C_3-6 carbocyclyl)(R²⁰)_j.

In some embodiments, at least one R¹² is —(C_4-6 carbocyclyl)(R²⁰)_j.

In some embodiments, at least one R¹² is —(C_5-6 carbocyclyl)(R²⁰)_j.

In some embodiments:

• • R¹ is -pyridinyl(R⁴)_q, wherein q is 1;
  • R² is H;
  • R³ is selected from the group consisting of -pyridinyl(R⁶)_q, wherein q is 0; and -aryl(R⁸)_k;
  • R⁴ is one substituent attached to the pyridinyl and is —NHC(═O)R¹²;
  • each R⁸ is one substituent attached to the aryl and is independently selected from the group consisting of Cl and F;
  • R¹² is independently selected from the group consisting of methyl, —(C_5-9 alkyl), -heteroaryl(R¹⁸)_q, -aryl(R¹⁹)_k, —CH₂aryl(R¹⁹)_k, —(C_4-6 carbocyclyl)(R²⁰)_j, and —CH₂carbocyclyl(R²⁰)_j;
  • each R¹⁸ is one substituent attached to the heteroaryl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R¹⁹ is one substituent attached to the aryl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R²⁰ is one substituent attached to the carbocyclyl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each q is independently 0 to 4;
  • each k is independently 0 to 5; and
  • each j is independently 0 to 12.

In some embodiments:

• • R¹ is -pyridinyl(R⁴)_q, wherein q is 1;
  • R² is H;
  • R³ is selected from the group consisting of -pyridinyl(R⁶)_q and -aryl(R⁸)_k;
  • R⁴ is one substituent attached to the pyridinyl and is —NHC(═O)R¹²;
  • each R⁶ is one substituent attached to the pyridinyl and is independently selected from the group consisting of —(C_1-6 alkyl), halide, —CF₃, —OCH₃, —CN, and —C(═O)R¹⁷;
  • each R⁸ is one substituent attached to the aryl and is independently selected from the group consisting of —(C_1-6 alkyl), Br, I, —CF₃, —CN, —OCH₃, —(C_1-6 alkylene)_pNHSO₂R¹⁷, —NR¹³(C_1-6 alkylene)NR¹³R¹⁴, —(C_1-6 alkylene)_pNR¹³R¹⁴, and —OR²⁵;
  • R¹² is selected from the group consisting of —(C_1-9 alkyl), -heteroaryl(R¹⁸)_q, -aryl(R¹⁹)_k, —CH₂aryl(R¹⁹)_k, -carbocyclyl(R²⁰)_j, and —CH₂carbocyclyl(R²⁰)_j;
  • each R¹³ is independently selected from the group consisting of H and —(C_1-6 alkyl);
  • each R¹⁴ is independently selected from the group consisting of H, —(C_1-6 alkyl), —CH₂aryl(R¹⁹)_k, and —CH₂carbocyclyl(R²⁰)_j;
  • each R¹⁷ is a —(C_1-6 alkyl);
  • each R¹⁸ is one substituent attached to the heteroaryl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R¹⁹ is one substituent attached to the aryl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R²⁰ is one substituent attached to the carbocyclyl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R²¹ is one substituent attached to the heterocyclyl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R²³ is independently selected from the group consisting of H and —(C_1-6 alkyl);
  • each R²⁴ is independently selected from the group consisting of H and —(C_1-6 alkyl);
  • R²⁵ is selected from the group consisting of H, —(C_1-6 alkyl), —(C_1-4 alkylene)_pheterocyclyl(R²¹)_h, and —(C_1-6 alkylene)_pNR²³R²⁴;
  • each p is independently 0 or 1;
  • each q is independently 0 to 4;
  • each k is independently 0 to 5; and
  • each j is independently 0 to 12.

In some embodiments:

• • R¹ is -pyridinyl(R⁴)_q, wherein q is 0 or 1;
  • R² is H;
  • R³ is selected from the group consisting of -pyridinyl(R⁶)_q and -aryl(R⁸)_k;
  • R⁴ is one substituent attached to the pyridinyl and is selected from the group consisting of halide, —(C_1-6 alkyl), —(C_1-4 alkylene)_pheterocyclyl(R⁹)_h, —(C_1-4 alkylene)_pcarbocyclyl(R¹⁰)_j, —(C_1-4 alkylene)_paryl(R¹¹)_k, —NR¹³R¹⁴, —(C_1-6 alkylene)NR¹⁵R¹⁶, and —OR²²;
  • each R⁶ is one substituent attached to the pyridinyl and is independently selected from the group consisting of —(C_1-6 alkyl), halide, —CF₃, —OCH₃, —CN, and —C(═O)R¹⁷;
  • each R⁸ is one substituent attached to the aryl and is independently selected from the group consisting of —(C_1-6 alkyl), halide, —CF₃, —CN, —OCH₃, —(C_1-6 alkylene)_pNHSO₂R¹⁷, —NR¹³(C_1-6 alkylene)NR¹³R¹⁴, —(C_1-6 alkylene)_pNR¹³R¹⁴, and —OR²⁵;
  • each R⁹ is one substituent attached to the heterocyclyl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R¹⁰ is one substituent attached to the carbocyclyl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R¹¹ is one substituent attached to the aryl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R¹³ is independently selected from the group consisting of H and —(C_1-6 alkyl);
  • each R¹⁴ is independently selected from the group consisting of H, —(C_1-6 alkyl), —CH₂aryl(R¹⁹)_k, and —CH₂carbocyclyl(R²⁰)_j;
  • each R¹⁵ is independently selected from the group consisting of H and —(C_1-6 alkyl);
  • each R¹⁶ is independently selected from the group consisting of H, —(C_1-6 alkyl), —CH₂aryl(R¹⁹)_k, and —CH₂carbocyclyl(R²⁰)_j;
  • each R¹⁷ is a —(C_1-6 alkyl);
  • each R¹⁸ is one substituent attached to the heteroaryl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R¹⁹ is one substituent attached to the aryl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R²⁰ is one substituent attached to the carbocyclyl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R²¹ is one substituent attached to the heterocyclyl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • R²² is selected from the group consisting of H, —(C_1-6 alkyl), —(C_1-4 alkylene)_pheterocyclyl(R²¹)_h, —(C_1-4 alkylene)_pcarbocyclyl(R²⁰)_j, —(C_1-4 alkylene)_paryl(R¹⁹)_k, and —(C_1-6 alkylene)_pNR²³R²⁴;
  • each R²³ is independently selected from the group consisting of H and —(C_1-6 alkyl);
  • each R²⁴ is independently selected from the group consisting of H and —(C_1-6 alkyl);
  • R²⁵ is selected from the group consisting of H, —(C_1-6 alkyl), —(C_1-4 alkylene)_pheterocyclyl(R²¹)_h, and —(C_1-6 alkylene)_pNR²³R²⁴;
  • each p is independently 0 or 1;
  • each q is independently 0 to 4;
  • each h is independently 0 to 10;
  • each k is independently 0 to 5; and
  • each j is independently 0 to 12.

In some embodiments:

• • R¹ is -pyridinyl(R⁴)_q, wherein q is 0 or 1;
  • R² is H;
  • R³ is selected from the group consisting of -pyridinyl(R⁶)_q and -aryl(R⁸)_k;
  • R⁴ is one substituent attached to the pyridinyl and is selected from the group consisting of halide, —(C_1-6 alkyl), —(C_1-4 alkylene)_pheterocyclyl(R⁹)_h, —(C_1-4 alkylene)_pcarbocyclyl(R¹⁰)_j, —(C_1-4 alkylene)_paryl(R¹¹)_k, —NR¹³R¹⁴, —(C_1-6 alkylene)NR¹⁵R¹⁶, and —OR²²;
  • each R⁶ is one substituent attached to the pyridinyl and is independently selected from the group consisting of —(C_1-6 alkyl), halide, —CF₃, —OCH₃, —CN, and —C(═O)R¹⁷;
  • each R⁸ is one substituent attached to the aryl and is independently selected from the group consisting of —(C_1-6 alkyl), halide, —CF₃, —CN, —OCH₃, —(C_1-6 alkylene)_pNHSO₂R¹⁷, —NR¹³(C_1-6 alkylene)NR¹³R¹⁴, —(C_1-6 alkylene)_pNR¹³R¹⁴, and —OR²⁵;
  • each R⁹ is one substituent attached to the heterocyclyl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R¹⁰ is one substituent attached to the carbocyclyl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R¹¹ is one substituent attached to the aryl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R¹³ is independently selected from the group consisting of H and —(C_1-6 alkyl);
  • each R¹⁴ is independently selected from the group consisting of H, —(C_1-6 alkyl), —CH₂aryl(R¹⁹)_k, and —CH₂carbocyclyl(R²⁰)_j;
  • each R¹⁵ is independently selected from the group consisting of H and —(C_1-6 alkyl);
  • each R¹⁶ is independently selected from the group consisting of H, —(C_1-6 alkyl), —CH₂aryl(R¹⁹)_k, and —CH₂carbocyclyl(R²⁰)_j;
  • each R¹⁷ is a —(C_1-6 alkyl);
  • each R¹⁸ is one substituent attached to the heteroaryl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R¹⁹ is one substituent attached to the aryl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R²⁰ is one substituent attached to the carbocyclyl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R²¹ is one substituent attached to the heterocyclyl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • R²² is selected from the group consisting of H, —(C_1-6 alkyl), —(C_1-4 alkylene)_pheterocyclyl(R²¹)_h, —(C_1-4 alkylene)_pcarbocyclyl(R²⁰)_j, —(C_1-4 alkylene)_paryl(R¹⁹)_k, and —(C_1-6 alkylene)_pNR²³R²⁴;
  • each R²³ is independently selected from the group consisting of H and —(C_1-6 alkyl);
  • each R²⁴ is independently selected from the group consisting of H and —(C_1-6 alkyl);
  • R²⁵ is selected from the group consisting of H, —(C_1-6 alkyl), —(C_1-4 alkylene)_pheterocyclyl(R²¹)_h, and —(C_1-6 alkylene)_pNR²³R²⁴;
  • each p is independently 0 or 1;
  • each q is independently 0 to 4;
  • each h is independently 0 to 10;
  • each k is independently 0 to 5; and
  • each j is independently 0 to 12.

In some embodiments:

• • R¹ is -pyridinyl(R⁴)_q, wherein q is 0 or 1;
  • R² is H;
  • R³ is H;
  • R⁴ is one substituent attached to the pyridinyl and is selected from the group consisting of halide, —(C_2-6 alkyl), —(C_1-4 alkylene)_pheterocyclyl(R⁹)_h, —(C_1-4 alkylene)_pcarbocyclyl(R¹⁰)_j, —(C_1-4 alkylene)_paryl(R¹¹)_k, —NHC(═O)R¹², —NR¹³R¹⁴, —(C_1-6 alkylene)NR¹⁵R¹⁶, and —OR²²;
  • each R⁹ is one substituent attached to the heterocyclyl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R¹⁰ is one substituent attached to the carbocyclyl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R¹¹ is one substituent attached to the aryl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • R¹² is selected from the group consisting of —(C_1-2 alkyl), —(C_5-9 alkyl), -heteroaryl(R¹⁸)_q, -aryl(R¹⁹)_k, —CH₂aryl(R¹⁹)_k, -carbocyclyl(R²⁰)_j, and —CH₂carbocyclyl(R²⁰)_j;
  • each R¹³ is independently selected from the group consisting of H and —(C_1-6 alkyl);
  • each R¹⁴ is independently selected from the group consisting of H, —(C_1-6 alkyl), —CH₂aryl(R¹⁹)_k, and —CH₂carbocyclyl(R²⁰)_j;
  • each R¹⁵ is independently selected from the group consisting of H and —(C_1-6 alkyl);
  • each R¹⁶ is independently selected from the group consisting of H, —(C_1-6 alkyl), —CH₂aryl(R¹⁹)_k, and —CH₂carbocyclyl(R²⁰)_j;
  • each R¹⁸ is one substituent attached to the heteroaryl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R¹⁹ is one substituent attached to the aryl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R²⁰ is one substituent attached to the carbocyclyl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R²¹ is one substituent attached to the heterocyclyl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • R²² is selected from the group consisting of H, —(C_1-6 alkyl), —(C_1-4 alkylene)_pheterocyclyl(R²¹)_h, —(C_1-4 alkylene)_pcarbocyclyl(R²⁰)_j, —(C_1-4 alkylene)_paryl(R¹⁹)_k, and —(C_1-6 alkylene)_pNR²³R²⁴;
  • R²³ is selected from the group consisting of H and —(C_1-6 alkyl);
  • R²⁴ is selected from the group consisting of H and —(C_1-6 alkyl);
  • each p is independently 0 or 1;
  • each q is independently 0 to 4;
  • each h is independently 0 to 10;
  • each k is independently 0 to 5; and
  • each j is independently 0 to 12.

In some embodiments:

• • R¹ is -pyridinyl(R⁴)_q, wherein q is 0 or 1;
  • R² is H;
  • R³ is -imidazolyl(R⁶)_q;
  • R⁴ is one substituent attached to the pyridinyl and is selected from the group consisting of halide, —(C_2-6 alkyl), —(C_1-4 alkylene)_pheterocyclyl(R⁹)_h, —(C_1-4 alkylene)_pcarbocyclyl(R¹⁰)_j, —(C_1-4 alkylene)_paryl(R¹¹)_k, —NHC(═O)R¹², —NR¹³R¹⁴, —(C_1-6 alkylene)NR¹⁵R¹⁶, and —OR²²;
  • each R⁶ is one substituent attached to the imidazolyl and is independently selected from the group consisting of —(C_1-6 alkyl), halide, —CF₃, —OCH₃, —CN, and —C(═O)R¹⁷;
  • each R⁹ is one substituent attached to the heterocyclyl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R¹⁰ is one substituent attached to the carbocyclyl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R¹¹ is one substituent attached to the aryl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • R¹² is selected from the group consisting of —(C_1-9 alkyl), -heteroaryl(R¹⁸)_q, -aryl(R¹⁹)_k, —CH₂aryl(R¹⁹)_k, -carbocyclyl(R²⁰)_j, and —CH₂carbocyclyl(R²⁰)_j;
  • R¹³ is selected from the group consisting of H and —(C_1-6 alkyl);
  • R¹⁴ is selected from the group consisting of H, —(C_1-6 alkyl), —CH₂aryl(R¹⁹)_k, and —CH₂carbocyclyl(R²⁰)_j;
  • R¹⁵ is selected from the group consisting of H and —(C_1-6 alkyl);
  • R¹⁶ is selected from the group consisting of H, —(C_1-6 alkyl), —CH₂aryl(R¹⁹)_k, and —CH₂carbocyclyl(R²⁰)_j;
  • each R¹⁷ is a —(C_1-6 alkyl);
  • each R¹⁸ is one substituent attached to the heteroaryl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R¹⁹ is one substituent attached to the aryl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R²⁰ is one substituent attached to the carbocyclyl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R²¹ is one substituent attached to the heterocyclyl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • R²² is selected from the group consisting of H, —(C_1-6 alkyl), —(C_1-4 alkylene)_pheterocyclyl(R²¹)_h, —(C_1-4 alkylene)_pcarbocyclyl(R²⁰)_j, —(C_1-4 alkylene)_paryl(R¹⁹)_k, and —(C_1-6 alkylene)_pNR²³R²⁴;
  • R²³ is selected from the group consisting of H and —(C_1-6 alkyl);
  • R²⁴ is selected from the group consisting of H and —(C_1-6 alkyl);
  • each p is independently an integer of 0 or 1;
  • each q is independently 0 to 4;
  • each h is independently 0 to 10;
  • each k is independently 0 to 5; and
  • each j is independently 0 to 12.

In some embodiments:

• • R¹ is -pyridinyl(R⁴)_q, wherein q is 0 or 1;
  • R² is H;
  • R³ is selected from the group consisting of -heteroaryl(R⁶)_q and -heterocyclyl(R⁷)_h, with the proviso that heteroaryl does not include pyridinyl and imidazolyl.
  • R⁴ is one substituent attached to the pyridinyl and is selected from the group consisting of halide, —(C_1-6 alkyl), —(C_1-4 alkylene)_pheterocyclyl(R⁹)_h, —(C_1-4 alkylene)_pcarbocyclyl(R¹⁰)_j, —(C_1-4 alkylene)_paryl(R¹¹)_k, —NHC(═O)R¹², —NR¹³R¹⁴, —(C_1-6 alkylene)NR¹⁵R¹⁶, and —OR²²;
  • each R⁶ is one substituent attached to the heteroaryl and is independently selected from the group consisting of —(C_1-6 alkyl), halide, —CF₃, —OCH₃, —CN, and —C(═O)R¹⁷;
  • each R⁷ is one substituent attached to the heterocyclyl and is independently selected from the group consisting of —(C_1-6 alkyl), halide, —CF₃, —CN, and —OCH₃;
  • each R⁹ is one substituent attached to the heterocyclyl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R¹⁰ is one substituent attached to the carbocyclyl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R¹¹ is one substituent attached to the aryl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • R¹² is selected from the group consisting of —(C_1-9 alkyl), -heteroaryl(R¹⁸)_q, -aryl(R¹⁹)_k, —CH₂aryl(R¹⁹)_k, -carbocyclyl(R²⁰)_j, and —CH₂carbocyclyl(R²⁰)_j;
  • R¹³ is selected from the group consisting of H and —(C_1-6 alkyl);
  • R¹⁴ is selected from the group consisting of H, —(C_1-6 alkyl), —CH₂aryl(R¹⁹)_k, and —CH₂carbocyclyl(R²⁰)_j;
  • R¹⁵ is selected from the group consisting of H and —(C_1-6 alkyl);
  • R¹⁶ is selected from the group consisting of H, —(C_1-6 alkyl), —CH₂aryl(R¹⁹)_k, and —CH₂carbocyclyl(R²⁰)_j;
  • each R¹⁷ is a —(C_1-6 alkyl);
  • each R¹⁸ is one substituent attached to the heteroaryl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R¹⁹ is one substituent attached to the aryl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R²⁰ is one substituent attached to the carbocyclyl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R²¹ is one substituent attached to the heterocyclyl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • R²² is selected from the group consisting of H, —(C_1-6 alkyl), —(C_1-4 alkylene)_pheterocyclyl(R²¹)_h, —(C_1-4 alkylene)_pcarbocyclyl(R²⁰)_j, —(C_1-4 alkylene)_paryl(R¹⁹)_k, and —(C_1-6 alkylene)_pNR²³R²⁴;
  • R²³ is selected from the group consisting of H and —(C_1-6 alkyl);
  • R²⁴ is selected from the group consisting of H and —(C_1-6 alkyl);
  • each p is independently 0 or 1;
  • each q is independently 0 to 4;
  • each h is independently 0 to 10;
  • each k is independently 0 to 5; and
  • each j is independently 0 to 12.

In some embodiments:

• • R¹ is selected from the group consisting of -pyrimidinyl(R⁴)_q, -pyrazinyl(R⁴)_q, -pyrazolyl(R⁴)_q, -imidazolyl(R⁴)_q, and -heterocyclyl(R⁵)_h;
  • R² is H;
  • R³ is selected from the group consisting of H, -heteroaryl(R⁶)_q, -heterocyclyl(R⁷)_h, and -aryl(R⁸)_k;
  • each R⁴ is one substituent attached to the heteroaryl and is independently selected from the group consisting of halide, —(C_1-6 alkyl), —(C_1-4 alkylene)_pheterocyclyl(R⁹)_h, —(C_1-4 alkylene)_pcarbocyclyl(R¹⁰)_j, —(C_1-4 alkylene)_paryl(R¹¹)_k, —NHC(═O)R¹², —NR¹³R¹⁴, —(C_1-6 alkylene)NR¹⁵R¹⁶, and —OR²²;
  • each R⁵ is one substituent attached to the heterocyclyl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R⁶ is one substituent attached to the heteroaryl and is independently selected from the group consisting of —(C_1-6 alkyl), halide, —CF₃, —OCH₃, —CN, and —C(═O)R¹⁷;
  • each R⁷ is one substituent attached to the heterocyclyl and is independently selected from the group consisting of —(C_1-6 alkyl), halide, —CF₃, —CN, and —OCH₃;
  • each R⁸ is one substituent attached to the aryl and is independently selected from the group consisting of —(C_1-6 alkyl), halide, —CF₃, —CN, —OCH₃, —(C_1-6 alkylene)_pNHSO₂R¹⁷, —NR¹³(C_1-6 alkylene)NR¹³R¹⁴, —(C_1-6 alkylene)_pNR¹³R¹⁴, and —OR²⁵;
  • each R⁹ is one substituent attached to the heterocyclyl and is independently selected from the group consisting of amino, —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R¹⁰ is one substituent attached to the carbocyclyl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R¹¹ is one substituent attached to the aryl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R¹² is independently selected from the group consisting of —(C_1-9 alkyl), -heteroaryl(R¹⁸)_q, -aryl(R¹⁹)_k, —CH₂aryl(R¹⁹)_k, -carbocyclyl(R²⁰)_j, —CH₂carbocyclyl(R²⁰)_j, —(C_1-4 alkylene)_pNR²³R²⁴, -heterocyclyl(R²¹)_h, and —CH₂heterocyclyl(R²¹)_h;
  • each R¹³ is independently selected from the group consisting of H and —(C_1-6 alkyl);
  • each R¹⁴ is independently selected from the group consisting of H, —(C_1-6 alkyl), —CH₂aryl(R¹⁹)_k, and —CH₂carbocyclyl(R²⁰)_j;
  • each R¹⁵ is independently selected from the group consisting of H and —(C_1-6 alkyl);
  • each R¹⁶ is independently selected from the group consisting of H, —(C_1-6 alkyl), —CH₂aryl(R¹⁹)_k, and —CH₂carbocyclyl(R²⁰)_j;
  • each R¹⁷ is a —(C_1-6 alkyl);
  • each R¹⁸ is one substituent attached to the heteroaryl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R¹⁹ is one substituent attached to the aryl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R²⁰ is one substituent attached to the carbocyclyl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R²¹ is one substituent attached to the heterocyclyl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • R²² is selected from the group consisting of H, —(C_1-6 alkyl), —(C_1-4 alkylene)_pheterocyclyl(R²¹)_h, —(C_1-4 alkylene)_pcarbocyclyl(R²⁰)_j, —(C_1-4 alkylene)_paryl(R¹⁹)_k, and —(C_1-6 alkylene)_pNR²³R²⁴;
  • each R²³ is independently selected from the group consisting of H and —(C_1-6 alkyl);
  • each R²⁴ is independently selected from the group consisting of H and —(C_1-6 alkyl);
  • R²⁵ is selected from the group consisting of H, —(C_1-6 alkyl), —(C_1-4 alkylene)_pheterocyclyl(R²¹)_h, and —(C_1-6 alkylene)_pNR²³R²⁴;
  • each p is independently 0 or 1;
  • each q is independently 0 to 4;
  • each h is independently 0 to 10;
  • each k is independently 0 to 5; and
  • each j is independently 0 to 12.

In some embodiments:

• • R¹ is selected from the group consisting of -heteroaryl(R⁴)_q and -heterocyclyl(R⁵)_h;
  • R² is a halide;
  • R³ is selected from the group consisting of H, -heteroaryl(R⁶)_q, -heterocyclyl(R⁷)_h, and -aryl(R⁸)_k;
  • each R⁴ is one substituent attached to the heteroaryl and is independently selected from the group consisting of halide, —(C_1-6 alkyl), —(C_1-4 alkylene)_pheterocyclyl(R⁹)_h, —(C_1-4 alkylene)_pcarbocyclyl(R¹⁰)_j, —(C_1-4 alkylene)_paryl(R¹¹)_k, —NHC(═O)R¹², —NR¹³R¹⁴, —(C_1-6 alkylene)NR¹⁵R¹⁶, and —OR²²;
  • each R⁵ is one substituent attached to the heterocyclyl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R⁶ is one substituent attached to the heteroaryl and is independently selected from the group consisting of —(C_1-6 alkyl), halide, —CF₃, —OCH₃, —CN, and —C(═O)R¹⁷;
  • each R⁷ is one substituent attached to the heterocyclyl and is independently selected from the group consisting of —(C_1-6 alkyl), halide, —CF₃, —CN, and —OCH₃;
  • each R⁸ is one substituent attached to the aryl and is independently selected from the group consisting of —(C_1-6 alkyl), halide, —CF₃, —CN, —OCH₃, —(C_1-6 alkylene)_pNHSO₂R¹⁷, —NR¹³(C_1-6 alkylene)NR¹³R¹⁴, —(C_1-6 alkylene)_pNR¹³R¹⁴, and —OR²⁵;
  • each R⁹ is one substituent attached to the heterocyclyl and is independently selected from the group consisting of amino, —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R¹⁰ is one substituent attached to the carbocyclyl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R¹¹ is one substituent attached to the aryl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R¹² is independently selected from the group consisting of —(C_1-9 alkyl), -heteroaryl(R¹⁸)_q, -aryl(R¹⁹)_k, —CH₂aryl(R¹⁹)_k, -carbocyclyl(R²⁰)_j, —CH₂carbocyclyl(R²⁰)_j, —(C_1-4 alkylene)_pNR²³R²⁴, -heterocyclyl(R²¹)_h, and —CH₂heterocyclyl(R²¹)_h;
  • each R¹³ is independently selected from the group consisting of H and —(C_1-6 alkyl);
  • each R¹⁴ is independently selected from the group consisting of H, —(C_1-6 alkyl), —CH₂aryl(R¹⁹)_k, and —CH₂carbocyclyl(R²⁰)_j;
  • each R¹⁵ is independently selected from the group consisting of H and —(C_1-6 alkyl);
  • each R¹⁶ is independently selected from the group consisting of H, —(C_1-6 alkyl), —CH₂aryl(R¹⁹)_k, and —CH₂carbocyclyl(R²⁰)_j;
  • each R¹⁷ is a —(C_1-6 alkyl);
  • each R¹⁸ is one substituent attached to the heteroaryl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R¹⁹ is one substituent attached to the aryl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R²⁰ is one substituent attached to the carbocyclyl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • each R²¹ is one substituent attached to the heterocyclyl and is independently selected from the group consisting of —(C_1-4 alkyl), halide, —CF₃, and —CN;
  • R²² is selected from the group consisting of H, —(C_1-6 alkyl), —(C_1-4 alkylene)_pheterocyclyl(R²¹)_h, —(C_1-4 alkylene)_pcarbocyclyl(R²⁰)_j, —(C_1-4 alkylene)_paryl(R¹⁹)_k, and —(C_1-6 alkylene)_pNR²³R²⁴;
  • each R²³ is independently selected from the group consisting of H and —(C_1-6 alkyl);
  • each R²⁴ is independently selected from the group consisting of H and —(C_1-6 alkyl);
  • R²⁵ is selected from the group consisting of H, —(C_1-6 alkyl), —(C_1-4 alkylene)_pheterocyclyl(R²¹)_h, and —(C_1-6 alkylene)_pNR²³R²⁴;
  • each p is independently 0 or 1;
  • each q is independently 0 to 4;
  • each h is independently 0 to 10;
  • each k is independently 0 to 5; and
  • each j is independently 0 to 12.

In some embodiments, R² is H; R¹ is -pyridin-3-yl(R⁴)_q; q is 1; R⁴ is —NHC(═O)R¹²; R¹² is —(C_2-5 alkyl); R³ is -phenyl(R⁸)_k; k is 1 or 2; and R⁸ is F.

In some embodiments, R² is H; R¹ is -pyridin-3-yl(R⁴)_q; q is 1; R⁴ is —NHC(═O)R¹²; R¹² is —(C_2-5 alkyl); R³ is -phenyl(R⁸)_k; k is 2; one R⁸ is F and the other R⁸ is —(C_1-2 alkylene)_pNHSO₂R¹⁷; p is 1; and R¹⁷ is —(C_1-3 alkyl).

In some embodiments, R² is H; R¹ is -pyridin-3-yl(R⁴)_q; q is 1; R⁴ is —NHC(═O)R¹²; R¹² is —(C_2-5 alkyl); R³ is -phenyl(R⁸)_k; k is 2; one R⁸ is F and the other R⁸ is —NH(C_1-6 alkylene)NR¹³R¹⁴; and R¹³ and R¹⁴ are independently selected from —(C_1-3 alkyl).

In some embodiments, R² is H; R¹ is -pyridin-3-yl(R⁴)_q, wherein q is 1; R⁴ is —NHC(═O)R¹²; R¹² is —(C_2-5 alkyl); R³ is -heteroaryl(R⁶)_q, wherein q is 1; R⁶ is selected from the group consisting of halide, —(C_1-2 alkyl), and —C(═O)R¹⁷; R¹⁷ is —(C_1-3 alkyl); and the heteroaryl is selected from the group consisting of pyridine, furan, thiophene, and imidazole.

In some embodiments, R² is H; R¹ is -pyridin-3-yl(R⁴)_q; q is 1; R⁴ is —NHC(═O)R¹²; R¹² is —(C_2-5 alkyl); R³ is -heterocyclyl(R⁷)_h; h is 1 or 2; and R⁷ is selected from the group consisting of halide and —(C_1-2 alkyl).

In some embodiments, R² is H; R¹ is -pyridin-3-yl(R⁴)_q; q is 1; R⁴ is —NHC(═O)R¹²; R¹² is -carbocyclyl(R²⁰)_j; j is 0; R³ is -phenyl(R⁸)_k; k is 1 or 2; R⁸ is F; and the carbocyclyl is selected from the group consisting of cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

In some embodiments, R² is H; R¹ is -pyridin-3-yl(R⁴)_q; q is 1; R⁴ is —NHC(═O)R¹²; R¹² is -carbocyclyl(R²⁰)_j; j is 0; R³ is -phenyl(R⁸)_k; k is 2; one R⁸ is F and the other R⁸ is —(C_1-2 alkylene)_pNHSO₂R¹⁷; p is 1; R¹⁷ is —(C_1-3 alkyl); and the carbocyclyl is selected from the group consisting of cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

In some embodiments, R² is H; R¹ is -pyridin-3-yl(R⁴)_q; q is 1; R⁴ is —NHC(═O)R¹²; R¹² is -carbocyclyl(R²⁰)_j; j is 0; R³ is -phenyl(R⁸)_k; k is 2; one R⁸ is F and the other R⁸ is —NH(C_1-6 alkylene)NR¹³R¹⁴; R¹³ and R¹⁴ are independently selected from —(C_1-3 alkyl); and the carbocyclyl is selected from the group consisting of cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

In some embodiments, R² is H; R¹ is -pyridin-3-yl(R⁴)_q, wherein q is 1; R⁴ is —NHC(═O)R¹²; R¹² is -carbocyclyl(R²⁰)_j; j is 0; R³ is -heteroaryl(R⁶)_q, wherein q is 1; R⁶ is selected from the group consisting of halide, —(C_1-2 alkyl), and —C(═O)R¹⁷; R¹⁷ is C_1-3 alkyl; the heteroaryl is selected from the group consisting of pyridine, furan, thiophene, and imidazole; and the carbocyclyl is selected from the group consisting of cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

In some embodiments, R² is H; R¹ is -pyridin-3-yl(R⁴)_q; q is 1; R⁴ is —NHC(═O)R¹²; R¹² is -carbocyclyl(R²⁰)_j; j is 0; R³ is -heterocyclyl(R⁷)_h; h is 1 or 2; R⁷ is selected from the group consisting of halide and —(C_1-2 alkyl); and the carbocyclyl is selected from the group consisting of cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

In some embodiments, R² is H; R¹ is -pyridin-3-yl(R⁴)_q; q is 1; R⁴ is selected from the group consisting of —NR¹³R¹⁴ and —CH₂NR¹⁵R¹⁶; R¹³ and R¹⁵ are independently selected from the group consisting of H and —(C_1-3 alkyl); R¹⁴ and R¹⁶ are independently selected from the group consisting of H, —(C_1-3 alkyl), —CH₂phenyl(R¹⁹)_k, and —CH₂carbocyclyl(R²⁰)_j, wherein j and k are 0; R³ is -phenyl(R⁸)_k, wherein k is 1 or 2; R⁸ is F; and the carbocyclyl is selected from the group consisting of cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

In some embodiments, R² is H; R¹ is -pyridin-3-yl(R⁴)_q; q is 1; R⁴ is selected from the group consisting of —NR¹³R¹⁴ and —CH₂NR¹⁵R¹⁶; R¹³ and R¹⁵ are independently selected from the group consisting of H and —(C_1-3 alkyl); R¹⁴ and R¹⁶ are independently selected from the group consisting of H, —(C_1-3 alkyl), —CH₂phenyl(R¹⁹)_k, and —CH₂carbocyclyl(R²⁰)_j, wherein j and k are 0; R³ is -phenyl(R⁸)_k, wherein k is 2; one R⁸ is F and the other R⁸ is —(C_1-2 alkylene)_pNHSO₂R¹⁷; p is 1; R¹⁷ is —(C_1-3 alkyl); and the carbocyclyl is selected from the group consisting of cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

In some embodiments, R² is H; R¹ is -pyridin-3-yl(R⁴)_q; q is 1; R⁴ is selected from the group consisting of —NR¹³R¹⁴ and —CH₂NR¹⁵R¹⁶, wherein R¹³ and R¹⁵ are independently selected from the group consisting of H and —(C_1-3 alkyl), and R¹⁴ and R¹⁶ are independently selected from the group consisting of H, —(C_1-3 alkyl), —CH₂phenyl(R¹⁹)_k, and —CH₂carbocyclyl(R²⁰)_j, wherein k and j are 0; R³ is -phenyl(R⁸)_k, wherein k is 2; one R⁸ is F and the other R⁸ is —NH(C_1-6 alkylene)NR¹³R¹⁴, wherein R¹³ and R¹⁴ are independently selected from —(C_1-3 alkyl); and the carbocyclyl is selected from the group consisting of cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

In some embodiments, R² is H; R¹ is -pyridin-3-yl(R⁴)_q, wherein q is 1; R⁴ is selected from the group consisting of —NR¹³R¹⁴ and —CH₂NR¹⁵R¹⁶; R¹³ and R¹⁵ are independently selected from the group consisting of H and —(C_1-3 alkyl); R¹⁴ and R¹⁶ are independently selected from the group consisting of H, —(C_1-3 alkyl), —CH₂phenyl(R¹⁹)_k, and —CH₂carbocyclyl(R²⁰)_j; k and j are 0; R³ is -heteroaryl(R⁶)_q, wherein q is 1; R⁶ is selected from the group consisting of halide, —(C_1-2 alkyl), and —C(═O)R¹⁷; R¹⁷ is —(C_1-3 alkyl); the heteroaryl is selected from the group consisting of pyridine, furan, thiophene, and imidazole; and the carbocyclyl is selected from the group consisting of cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

In some embodiments, R² is H; R¹ is -pyridin-3-yl(R⁴)_q; q is 1; R⁴ is selected from the group consisting of —NR¹³R¹⁴ and —CH₂NR¹⁵R¹⁶; R¹³ and R¹⁵ are independently selected from the group consisting of H and —(C_1-3 alkyl); R¹⁴ and R¹⁶ are independently selected from the group consisting of H, —(C_1-3 alkyl), —CH₂phenyl(R¹⁹)_k, and —CH₂carbocyclyl(R²⁰)_j; k and j are 0; R³ is -heterocyclyl(R⁷)_h; h is 1 or 2; R¹ is selected from the group consisting of halide and —(C_1-2 alkyl); and the carbocyclyl is selected from the group consisting of cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

In some embodiments, R² is H; R¹ is -pyridin-3-yl(R⁴)_q; q is 1; R⁴ is —CH₂heterocyclyl(R⁹)_h; h is 0-2; R⁹ is F; R³ is -phenyl(R⁸)_k; k is 1 or 2; R⁸ is F; and the heterocyclyl is selected from the group consisting of pyrrolidine and piperidine.

In some embodiments, R² is H; R¹ is -pyridin-3-yl(R⁴)_q; q is 1; R⁴ is —CH₂heterocyclyl(R⁹)_h; h is 0-2; R⁹ is F; R³ is -phenyl(R⁸)_k; k is 2; one R⁸ is F and the other R⁸ is —(C_1-2 alkylene)_pNHSO₂R¹⁷; p is 1; R¹⁷ is —(C_1-3 alkyl); and the heterocyclyl is selected from the group consisting of pyrrolidine and piperidine.

In some embodiments, R² is H; R¹ is -pyridin-3-yl(R⁴)_q; q is 1; R⁴ is —CH₂heterocyclyl(R⁹)_h; h is 0-2; R⁹ is F; R³ is -phenyl(R⁸)_k; k is 2; and R⁸ is one F and the other R⁸—NH(C_1-6 alkylene)NR¹³R¹⁴; R¹³ and R¹⁴ are independently selected from —(C_1-3 alkyl); and the heterocyclyl is selected from the group consisting of pyrrolidine and piperidine.

In some embodiments, R² is H; R¹ is -pyridin-3-yl(R⁴)_q, wherein q is 1; R⁴ is —CH₂heterocyclyl(R⁹)_h; h is 0-2; R⁹ is F; R³ is -heteroaryl(R⁶)_q, wherein q is 1; R⁶ is selected from the group consisting of halide, —(C_1-2 alkyl), and —C(═O)R¹⁷; R¹⁷ is —(C_1-3 alkyl); the heteroaryl is selected from the group consisting of pyridine, furan, thiophene, and imidazole; and the heterocyclyl is selected from the group consisting of pyrrolidine and piperidine.

In some embodiments, R² is H; R¹ is -pyridin-3-yl(R⁴)_q; q is 1; R⁴ is —CH₂heterocyclyl(R⁹)_h; h is 0-2; R⁹ is F; R³ is -heterocyclyl(R⁷)_h; h is 1 or 2; R⁷ is selected from the group consisting of halide and —(C_1-2 alkyl); and the heterocyclyl is selected from the group consisting of pyrrolidine and piperidine.

In some embodiments, R² is H; R¹ is -pyrimidinyl(R⁴)_q; q is 0; R³ is -phenyl(R⁸)_k; k is 1 or 2; and R⁸ is F.

In some embodiments, R² is H; R¹ is -pyrimidinyl(R⁴)_q; q is 0; R³ is -phenyl(R⁸)_k; k is 2; one R⁸ is F and the other R⁸ is —(C_1-2 alkylene)_pNHSO₂R¹⁷; p is 1; and R¹⁷ is —(C_1-3 alkyl).

In some embodiments, R² is H; R¹ is -pyrimidinyl(R⁴)_q; q is 0; R³ is -phenyl(R⁸)_k; k is 2; one R⁸ is F and the other R⁸ is —NH(C_1-6 alkylene)NR¹³R¹⁴; and R¹³ and R¹⁴ are independently selected from —(C_1-3 alkyl).

In some embodiments, R² is H; R¹ is -pyrimidinyl(R⁴)_q, wherein q is 0; R³ is -heteroaryl(R⁶)_q, wherein q is 1; R⁶ is selected from the group consisting of halide, —(C_1-2 alkyl), and —C(═O)R¹⁷; R¹⁷ is —(C_1-3 alkyl); and the heteroaryl is selected from the group consisting of pyridine, furan, thiophene, and imidazole.

In some embodiments, R² is H; R¹ is -pyrimidinyl(R⁴)_q; q is 0; R³ is -heterocyclyl(R⁷)_h; h is 1 or 2; R⁷ is selected from the group consisting of halide and —(C_1-2 alkyl).

In some embodiments, R² is F; R¹ is -pyridin-3-yl(R⁴)_q; q is 1; R⁴ is —NHC(═O)R¹²; R¹² is —(C_2-5 alkyl); R³ is -phenyl(R⁸)_k; k is 1 or 2; and R⁸ is F.

In some embodiments, R² is F; R¹ is -pyridin-3-yl(R⁴)_q; q is 1; R⁴ is —NHC(═O)R¹²; R¹² is —(C_2-5 alkyl); R³ is -phenyl(R⁸)_k; k is 2; one R⁸ is F and the other R⁸ is —(C_1-2 alkylene)_pNHSO₂R¹⁷; p is 1; and R¹⁷ is —(C_1-3 alkyl).

In some embodiments, R² is F; R¹ is -pyridin-3-yl(R⁴)_q; q is 1; R⁴ is —NHC(═O)R¹²; R¹² is —(C_2-5 alkyl); R³ is -phenyl(R⁸)_k; k is 2; one R⁸ is F and the other R⁸ is —NH(C_1-6 alkylene)NR¹³R¹⁴; and R¹³ and R¹⁴ are independently selected from —(C_1-3 alkyl).

In some embodiments, R² is F; R¹ is -pyridin-3-yl(R⁴)_q, wherein q is 1; R⁴ is —NHC(═O)R¹²; R¹² is —(C_2-5 alkyl); R³ is -heteroaryl(R⁶)_q, wherein q is 1; R⁶ is selected from the group consisting of halide, —(C_1-2 alkyl), and —C(═O)R¹⁷; R¹⁷ is —(C_1-3 alkyl); and the heteroaryl is selected from the group consisting of pyridine, furan, thiophene, and imidazole.

In some embodiments, R² is F; R¹ is -pyridin-3-yl(R⁴)_q; q is 1; R⁴ is —NHC(═O)R¹²; R¹² is —(C_2-5 alkyl); R³ is -heterocyclyl(R⁷)_h; h is 1 or 2; and R⁷ is selected from the group consisting of halide and —(C_1-2 alkyl).

In some embodiments, R² is F; R¹ is -pyridin-3-yl(R⁴)_q; q is 1; R⁴ is —NHC(═O)R¹²; R¹² is -carbocyclyl(R²⁰)_j; j is 0; R³ is -phenyl(R⁸)_k; k is 1 or 2; R⁸ is F; and the carbocyclyl is selected from the group consisting of cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

In some embodiments, R² is F; R¹ is -pyridin-3-yl(R⁴)_q; q is 1; R⁴ is —NHC(═O)R¹²; R¹² is -carbocyclyl(R²⁰)_j; j is 0; R³ is -phenyl(R⁸)_k; k is 2; one R⁸ is F and the other R⁸ is —(C_1-2 alkylene)_pNHSO₂R¹⁷; p is 1; R¹⁷ is —(C_1-3 alkyl); and the carbocyclyl is selected from the group consisting of cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

In some embodiments, R² is F; R¹ is -pyridin-3-yl(R⁴)_q; q is 1; R⁴ is —NHC(═O)R¹²; R¹² is -carbocyclyl(R²⁰)_j; j is 0; R³ is -phenyl(R⁸)_k; k is 2; one R⁸ is F and the other R⁸ is —NH(C_1-6 alkylene)NR¹³R¹⁴; R¹³ and R¹⁴ are independently selected from —(C_1-3 alkyl); and the carbocyclyl is selected from the group consisting of cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

In some embodiments, R² is F; R¹ is -pyridin-3-yl(R⁴)_q, wherein q is 1; R⁴ is —NHC(═O)R¹²; R¹² is -carbocyclyl(R²⁰)_j; j is 0; R³ is -heteroaryl(R⁶)_q, wherein q is 1; R⁶ is selected from the group consisting of halide, —(C_1-2 alkyl), and —C(═O)R¹⁷; R¹⁷ is C_1-3 alkyl; the heteroaryl is selected from the group consisting of pyridine, furan, thiophene, and imidazole; and the carbocyclyl is selected from the group consisting of cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

In some embodiments, R² is F; R¹ is -pyridin-3-yl(R⁴)_q; q is 1; R⁴ is —NHC(═O)R¹²; R¹² is -carbocyclyl(R²⁰)_j; j is 0; R³ is -heterocyclyl(R⁷)_h; h is 1 or 2; R is selected from the group consisting of halide and —(C_1-2 alkyl); and the carbocyclyl is selected from the group consisting of cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

In some embodiments, R² is F; R¹ is -pyridin-3-yl(R⁴)_q; q is 1; R⁴ is selected from the group consisting of —NR¹³R¹⁴ and —CH₂NR¹⁵R¹⁶; R¹³ and R¹⁵ are independently selected from the group consisting of H and —(C_1-3 alkyl); R¹⁴ and R¹⁶ are independently selected from the group consisting of H, —(C_1-3 alkyl), —CH₂phenyl(R¹⁹)_k, and —CH₂carbocyclyl(R²⁰)_j, wherein k and j are 0; R³ is -phenyl(R⁸)_k, wherein k is 1 or 2; R⁸ is F; and the carbocyclyl is selected from the group consisting of cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

In some embodiments, R² is F; R¹ is -pyridin-3-yl(R⁴)_q; q is 1; R⁴ is selected from the group consisting of —NR¹³R¹⁴ and —CH₂NR¹⁵R¹⁶; R¹³ and R¹⁵ are independently selected from the group consisting of H and —(C_1-3 alkyl); R¹⁴ and R¹⁶ are independently selected from the group consisting of H, —(C_1-3 alkyl), —CH₂phenyl(R¹⁹)_k, and —CH₂carbocyclyl(R²⁰)_j, wherein k and j are 0; R³ is -phenyl(R⁸)_k, wherein k is 2; one R⁸ is F and the other R⁸ is —(C_1-2 alkylene)_pNHSO₂R¹⁷; p is 1; R¹⁷ is —(C_1-3 alkyl); and the carbocyclyl is selected from the group consisting of cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

In some embodiments, R² is F; R¹ is -pyridin-3-yl(R⁴)_q; q is 1; R⁴ is selected from the group consisting of —NR¹³R¹⁴ and —CH₂NR¹⁵R¹⁶, wherein R¹³ and R¹⁵ are independently selected from the group consisting of H and —(C_1-3 alkyl), and R¹⁴ and R¹⁶ are independently selected from the group consisting of H, —(C_1-3 alkyl), —CH₂phenyl(R¹⁹)_k, and —CH₂carbocyclyl(R²⁰)_j, wherein k and j are 0; R³ is -phenyl(R⁸)_k, wherein k is 2; one R⁸ is F and the other R⁸ is —NH(C_1-6 alkylene)NR¹³R¹⁴, wherein R¹³ and R¹⁴ are independently selected from —(C_1-3 alkyl); and the carbocyclyl is selected from the group consisting of cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

In some embodiments, R² is F; R¹ is -pyridin-3-yl(R⁴)_q, wherein q is 1; R⁴ is selected from the group consisting of —NR¹³R¹⁴ and —CH₂NR¹⁵R¹⁶; R¹³ and R¹⁵ are independently selected from the group consisting of H and —(C_1-3 alkyl); R¹⁴ and R¹⁶ are independently selected from the group consisting of H, —(C_1-3 alkyl), —CH₂phenyl(R¹⁹)_k, and —CH₂carbocyclyl(R²⁰)_j; k and j are 0; R³ is -heteroaryl(R⁶)_q, wherein q is 1; R⁶ is selected from the group consisting of halide, —(C_1-2 alkyl), and —C(═O)R¹⁷; R¹⁷ is —(C_1-3 alkyl); the heteroaryl is selected from the group consisting of pyridine, furan, thiophene, and imidazole; and the carbocyclyl is selected from the group consisting of cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

In some embodiments, R² is F; R¹ is -pyridin-3-yl(R⁴)_q; q is 1; R⁴ is selected from the group consisting of —NR³R⁴ and —CH₂NR¹⁵R¹⁶; R¹³ and R¹⁵ are independently selected from the group consisting of H and —(C_1-3 alkyl); R¹⁴ and R¹⁶ are independently selected from the group consisting of H, —(C_1-3 alkyl), —CH₂phenyl(R¹⁹)_k, and —CH₂carbocyclyl(R²⁰)_j; k and j are 0; R³ is -heterocyclyl(R⁷)_h; h is 1 or 2; R⁷ is selected from the group consisting of halide and —(C_1-2 alkyl); and the carbocyclyl is selected from the group consisting of cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

In some embodiments, R² is F; R¹ is -pyridin-3-yl(R⁴)_q; q is 1; R⁴ is —CH₂heterocyclyl(R⁹)_h; h is 0-2; R⁹ is F; R³ is -phenyl(R⁸)_k; k is 1 or 2; R⁸ is F; and the heterocyclyl is selected from the group consisting of pyrrolidine and piperidine.

In some embodiments, R² is F; R¹ is -pyridin-3-yl(R⁴)_q; q is 1; R⁴ is —CH₂heterocyclyl(R⁹)_h; h is 0-2; R⁹ is F; R³ is -phenyl(R⁸)_k; k is 2; one R⁸ is F and the other R⁸ is —(C_1-2 alkylene)_pNHSO₂R¹⁷; p is 1; R¹⁷ is —(C_1-3 alkyl); and the heterocyclyl is selected from the group consisting of pyrrolidine and piperidine.

In some embodiments, R² is F; R¹ is -pyridin-3-yl(R⁴)_q; q is 1; R⁴ is —CH₂heterocyclyl(R⁹)_h; h is 0-2; R⁹ is F; R³ is -phenyl(R⁸)_k; k is 2; and R⁸ is one F and the other R⁸ is —NH(C_1-6 alkylene)NR¹³R¹⁴; R¹³ and R¹⁴ are independently selected from —(C_1-3 alkyl); and the heterocyclyl is selected from the group consisting of pyrrolidine and piperidine.

In some embodiments, R² is F; R¹ is -pyridin-3-yl(R⁴)_q, wherein q is 1; R⁴ is —CH₂heterocyclyl(R⁹)_h; h is 0-2; R⁹ is F; R³ is -heteroaryl(R⁶)_q, wherein q is 1; R⁶ is selected from the group consisting of halide, —(C_1-2 alkyl), and —C(═O)R¹⁷; R¹⁷ is —(C_1-3 alkyl); the heteroaryl is selected from the group consisting of pyridine, furan, thiophene, and imidazole; and the heterocyclyl is selected from the group consisting of pyrrolidine and piperidine.

In some embodiments, R² is F; R¹ is -pyridin-3-yl(R⁴)_q; q is 1; R⁴ is —CH₂heterocyclyl(R⁹)_h, wherein h is 0-2; R⁹ is F; R³ is -heterocyclyl(R⁷)_h, wherein h is 1 or 2; R⁷ is selected from the group consisting of halide and —(C_1-2 alkyl); and the heterocyclyl is selected from the group consisting of pyrrolidine and piperidine.

In some embodiments, R² is F; R¹ is -pyrimidinyl(R⁴)_q; q is 0; R³ is -phenyl(R⁸)_k; k is 1 or 2; and R⁸ is F.

In some embodiments, R² is F; R¹ is -pyrimidinyl(R⁴)_q; q is 0; R³ is -phenyl(R⁸)_k; k is 2; one R⁸ is F and the other R⁸ is —(C_1-2 alkylene)_pNHSO₂R¹⁷; p is 1; and R¹⁷ is —(C_1-3 alkyl).

In some embodiments, R² is F; R¹ is -pyrimidinyl(R⁴)_q; q is 0; R³ is -phenyl(R⁸)_k; k is 2; one R⁸ is F and the other R⁸ is —NH(C_1-6 alkylene)NR¹³R¹⁴; and R¹³ and R¹⁴ are independently selected from —(C_1-3 alkyl).

In some embodiments, R² is F; R¹ is -pyrimidinyl(R⁴)_q, wherein q is 0; R³ is -heteroaryl(R⁶)_q, wherein q is 1; R⁶ is selected from the group consisting of halide, —(C_1-2 alkyl), and —C(═O)R¹⁷; R¹⁷ is —(C_1-3 alkyl); and the heteroaryl is selected from the group consisting of pyridine, furan, thiophene, and imidazole.

In some embodiments, R² is F; R¹ is -pyrimidinyl(R⁴)_q; q is 0; R³ is -heterocyclyl(R⁷)_h; h is 1 or 2; R⁷ is selected from the group consisting of halide and —(C_1-2 alkyl).

In some embodiments, R² is H; R¹ is -pyrazol-4-yl(R⁴)_q; q is 0 or 1; R⁴ is —(C_1-3 alkyl); R³ is -phenyl(R⁸)_k; k is 1 or 2; and R⁸ is F.

In some embodiments, R² is H; R¹ is -imidazol-5-yl(R⁴)_q; q is 1 or 2; each R⁴ is independently selected from —(C_1-3 alkyl); R³ is -phenyl(R⁸)_k; k is 1 or 2; and R⁸ is F.

In some embodiments, R² is H; R¹ is -pyridin-3-yl(R⁴)_q; q is 1; R⁴ is —OR²²; R²² is selected from the group consisting of H and —(C_1-3 alkyl); R³ is -phenyl(R⁸)_k; k is 1 or 2; and R⁸ is F.

Illustrative compounds of Formula (I) are shown in Table 1.

TABLE 1
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
138
139
140
141
142
143
144
145
146
147
148
149
150
151
152
153
154
155
156
157
158
159
160
161
162
163
164
165
166
167
168
169
170
171
172
173
174
175
176
177
178
179
180
181
182
183
184
185
186
187
188
189
190
191
192
193
194
195
196
197
198
199
200
201
202
203
204
205
206
207
208
209
210
211
212
213
214
215
216
217
218
219
220
221
222
223
224
225
226
227
228
229
230
231
232
233
234
235
236
237
238
239
240
241
242
243
244
245
246
247
248
249
250
251
252
253
254
255
256
257
258
259
260
261
262
263
264
265
266
267
268
269
270
271
272
273
274
275
276
277
278
279
280
281
282
283
284
285
286
287
288
289
290
291
292
293
294
295
296
297
298
299
300
301
302
303
304
305
306
307
308
309
310
311
312
313
314
315
316
317
318
319
320
321
322
323
324
325
326
327
328
329
330
331
332
333
334
335
336
337
338
339
340
341
342
343
344
345
346
347
348
349
350
351
352
353
354
355
356
357
358
359
360
361
362
363
364
365
366
367
368
369
370
371
372
373
374
375
376
377
378
379
380
381
382
383
384
385
386
387
388
389
390
391
392
393
394
395
396
397
398
399
400
401
402
403
404
405
406
407
408
409
410
411
412
413
414
415
416
417
418
419
420
421
422
423
424
425
426
427
428
429
430
431
432
433
434
435
436
437
438
439
440
441
442
443
444
445
446
447
448
449
450
451
452
453
454
455
456
457
458
459
460
461
462
463
464
465
466
467
468
469
470
471
472
473
474
475
476
477
478
479
480
481
482
483
484
485
486
487
488
489
490
491
492
493
494
495
496
497
498
499
500
501
502
503
504
505
506
507
508
509
510
511
512
513
514
515
516
517
518
519
520
521
522
523
524
525
526
527
528
529
530
531
532
533
534
535
536
537
538
539
540
541
542
543
544
545
546
547
548
549
550
551
552
553
554
555
556
557
558
559
560
561
562
563
564
565
566
567
568
569
570
571
572
573
574
575
576
577
578
579
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581
582
583
584
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589
590
591
592
593
594
595
596
597
598
599
600
601
602
603
604
605
606
607
608
609
610
611
612
613
614
615
616
617
618
619
620
621
622
623
624
625
626
627
628
629
630
631
632
633
634
635
636
637
638
639
640
641
642
643
644
645
646
647
648
649
650
651
652
653
654
655
656
657
658
659
660
661
662
663
664
665
666
667
668
669
670
671
672
673
674
675
676
677
678
679
680
681
682
683
684
685
686
687
688
689
690
691
692
693
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695
696
697
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702
703
704
705
706
707
708
709
710
711
712
713
714
715
716
717
718
719
720
721
722
723
724
725
726
727
728
729
730
731
732
733
734
735
736
737
738
739
740
741
742
743
744
745
746
747
748
749
750
751
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753
754
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756
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759
760
761
762
763
764
765
766
767
768
769
770
771
772
773
774
775
776
777
778
779
780
781
782
783
784
785
786
787
788
789
790
791
792
793
794
795
796
797
798
799
800
801
802
803
804
805
806
807
808
809
810
811
812
813
814
815
816
817
818
819
820
821
822
823
824
825
826
827
828
829
830
831
832
833
834
835
836
837
838
839
840
841
842
843
844
845
846
847
848
849
850
851
852
853
854
855
856
857
858
859
860
861
862
863
864
865
866
867
868
869
870
871
872
873
874
875
876
877
878
879
880
881
882
883
884
885
886
887
888
889
890
891
892
893
894
895
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898
899
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902
903
904
905
906
907
908
909
910
911
912
913
914
915
916
917
918
919
920
921
922
923
924
925
926
927
928
929
930
931
932
933
934
935
936
937
938
939
940
941
942
943
944
945
946
947
948
949
950
951
952
953
954
955
956
957
958
959
960
961
962
963
964
965
966
967
968
969
970
971
972
973
974
975
976
977
978
979
980
981
982
983
984
985
986
987
988
989
990
991
992
993
994
995
996
997
998
999
1000
1001
1002
1003
1004
1005
1006
1007
1008
1009
1010
1011
1012
1013
1014
1015
1016
1017
1018
1019
1020
1021
1022
1023
1024
1025
1026
1027
1028
1029
1030
1031
1032
1033
1034
1035
1036
1037
1038
1039
1040
1041
1042
1043
1044
1045
1046
1047
1048
1049
1050
1051
1052
1053
1054
1055
1056
1057
1058
1059
1060
1061
1062
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1064
1065
1066
1067
1068
1069
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1071
1072
1073
1074
1075
1076
1077
1078
1079
1080
1081
1082
1083
1084
1085
1086
1087
1088
1089
1090
1091
1092
1093
1094
1095
1096
1097
1098
1099
1100
1101
1102
1103
1104
1105
1106
1107
1108
1109
1110
1111
1112
1113
1114
1115
1116
1117
1118
1119
1120
1121
1122
1123
1124
1125
1126
1127
1128
1129
1130
1131
1132
1133
1134
1135
1136
1137
1138
1139
1140
1141
1142
1143
1144
1145
1146
1147
1148
1149
1150
1151
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1171
1172
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1174
1175
1176
1177
1178
1179
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1181
1182
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1184
1185
1186
1187
1188
1189
1190
1191
1192
1193
1194
1195
1196
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1198
1199
1200
1201
1202
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1204
1205
1206
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1211
1212
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1221
1222
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1231
1232
1233
1234
1235
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1372
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1444
1445
1446

Administration and Pharmaceutical Compositions

Some embodiments include pharmaceutical compositions comprising: (a) a therapeutically effective amount of a compound provided herein, or its corresponding enantiomer, diastereoisomer or tautomer, or pharmaceutically acceptable salt; and (b) a pharmaceutically acceptable carrier.

The compounds provided herein may also be useful in combination (administered together or sequentially) with other known agents.

Non-limiting examples of diseases which can be treated with a combination of a compound of Formula (I) and other known agents are colorectal cancer, ovarian cancer, retinitis pigmentosa, macular degeneration, diabetic retinopathy, idiopathic pulmonary fibrosis/pulmonary fibrosis, and osteoarthritis.

In some embodiments, colorectal cancer can be treated with a combination of a compound of Formula (I) and one or more of the following drugs: 5-Fluorouracil (5-FU), which can be administered with the vitamin-like drug leucovorin (also called folinic acid); capecitabine (XELODA®), irinotecan (CAMPOSTAR®), oxaliplatin (ELOXATIN®). Examples of combinations of these drugs which could be further combined with a compound of Formula (I) are FOLFOX (5-FU, leucovorin, and oxaliplatin), FOLFIRI (5-FU, leucovorin, and irinotecan), FOLFOXIRI (leucovorin, 5-FU, oxaliplatin, and irinotecan) and CapeOx (Capecitabine and oxaliplatin). For rectal cancer, chemo with 5-FU or capecitabine combined with radiation may be given before surgery (neoadjuvant treatment).

In some embodiments, ovarian cancer can be treated with a combination of a compound of Formula (I) and one or more of the following drugs: Topotecan, Liposomal doxorubicin (DOXIL®), Gemcitabine (GEMZAR®), Cyclophosphamide (CYTOXAN®), Vinorelbine (NAVELBINE®), Ifosfamide (IFEX®), Etoposide (VP-16), Altretamine (HEXALEN®), Capecitabine (XELODA®), Irinotecan (CPT-11, CAMPTOSAR®), Melphalan, Pemetrexed (ALIMTA®) and Albumin bound paclitaxel (nab-paclitaxel, ABRAXANE®). Examples of combinations of these drugs which could be further combined with a compound of Formula (I) are TIP (paclitaxel [Taxol], ifosfamide, and cisplatin), VeIP (vinblastine, ifosfamide, and cisplatin) and VIP (etoposide [VP-16], ifosfamide, and cisplatin).

In some embodiments, a compound of Formula (I) can be used to treat cancer in combination with any of the following methods: (a) Hormone therapy such as aromatase inhibitors, LHRH [luteinizing hormone-releasing hormone] analogs and inhibitors, and others; (b) Ablation or embolization procedures such as radiofrequency ablation (RFA), ethanol (alcohol) ablation, microwave thermotherapy and cryosurgery (cryotherapy); (c) Chemotherapy using alkylating agents such as cisplatin and carboplatin, oxaliplatin, mechlorethamine, cyclophosphamide, chlorambucil and ifosfamide; (d) Chemotherapy using anti-metabolites such as azathioprine and mercaptopurine; (e) Chemotherapy using plant alkaloids and terpenoids such as vinca alkaloids (i.e. Vincristine, Vinblastine, Vinorelbine and Vindesine) and taxanes; (f) Chemotherapy using podophyllotoxin, etoposide, teniposide and docetaxel; (g) Chemotherapy using topoisomerase inhibitors such as irinotecan, topotecan, amsacrine, etoposide, etoposide phosphate, and teniposide; (h) Chemotherapy using cytotoxic antibiotics such as actinomycin, anthracyclines, doxorubicin, daunorubicin, valrubicin, idarubicin, epirubicin, bleomycin, plicamycin and mitomycin; (i) Chemotherapy using tyrosine-kinase inhibitors such as Imatinib mesylate (GLEEVEC®, also known as STI-571), Gefitinib (Iressa, also known as ZD1839), Erlotinib (marketed as TARCEVA®), Bortezomib (VELCADE®), tamoxifen, tofacitinib, crizotinib, Bcl-2 inhibitors (e.g. obatoclax in clinical trials, ABT-263, and Gossypol), PARP inhibitors (e.g. Iniparib, Olaparib in clinical trials), PI3K inhibitors (eg. perifosine in a phase III trial), VEGF Receptor 2 inhibitors (e.g. Apatinib), AN-152, (AEZS-108), Braf inhibitors (e.g. vemurafenib, dabrafenib and LGX818), MEK inhibitors (e.g. trametinib and MEK162), CDK inhibitors, (e.g. PD-0332991), salinomycin and Sorafenib; (j) Chemotherapy using monoclonal antibodies such as Rituximab (marketed as MABTHERA® or RITUXAN®), Trastuzumab (Herceptin also known as ErbB2), Cetuximab (marketed as ERBITUX®), and Bevacizumab (marketed as AVASTIN®); and (k) radiation therapy.

In some embodiments, diabetic retinopathy can be treated with a combination of a compound of Formula (I) and one or more of the following natural supplements: Bilberry, Butcher's broom, Ginkgo, Grape seed extract, and Pycnogenol (Pine bark).

In some embodiments, idiopathic pulmonary fibrosis/pulmonary fibrosis can be treated with a combination of a compound of Formula (I) and one or more of the following drugs: pirfenidone (pirfenidone was approved for use in 2011 in Europe under the brand name Esbriet®), prednisone, azathioprine, N-acetylcysteine, interferon-γ 1b, bosentan (bosentan is currently being studied in patients with IPF, [The American Journal of Respiratory and Critical Care Medicine (2011), 184(1), 92-9]), Nintedanib (BIBF 1120 and Vargatef), QAX576 [British Journal of Pharmacology (2011), 163(1), 141-172], and anti-inflammatory agents such as corticosteroids.

In some embodiments, a compound of Formula (I) can be used to treat idiopathic pulmonary fibrosis/pulmonary fibrosis in combination with any of the following methods: oxygen therapy, pulmonary rehabilitation and surgery.

In some embodiments, a compound of Formula (I) can be used to treat osteoarthritis in combination with any of the following methods: (a) Nonsteroidal anti-inflammatory drugs (NSAIDs) such as ibuprofen, naproxen, aspirin and acetaminophen; (b) physical therapy; (c) injections of corticosteroid medications; (d) injections of hyaluronic acid derivatives (e.g. Hyalgan, Synvisc); (e) narcotics, like codeine; (f) in combination with braces and/or shoe inserts or any device that can immobilize or support your joint to help you keep pressure off it (e.g., splints, braces, shoe inserts or other medical devices); (g) realigning bones (osteotomy); (h) joint replacement (arthroplasty); and (i) in combination with a chronic pain class.

In some embodiments, macular degeneration can be treated with a combination of a compound of Formula (I) and one or more of the following drugs: Bevacizumab (Avastin®), Ranibizumab (Lucentis®), Pegaptanib (Macugen), Aflibercept (Eylea®), verteporfin (Visudyne®) in combination with photodynamic therapy (PDT) or with any of the following methods: (a) in combination with laser to destroy abnormal blood vessels (photocoagulation); and (b) in combination with increased vitamin intake of antioxidant vitamins and zinc.

In some embodiments, retinitis pigmentosa can be treated with a combination of a compound of Formula (I) and one or more of the following drugs: UF-021 (Ocuseva™), vitamin A palmitate and pikachurin or with any of the following methods: (a) with the Argus® II retinal implant; and (b) with stem cell and/or gene therapy.

Administration of the compounds disclosed herein or the pharmaceutically acceptable salts thereof can be via any of the accepted modes of administration, including, but not limited to, orally, subcutaneously, intravenously, intranasally, topically, transdermally, intraperitoneally, intramuscularly, intrapulmonarilly, vaginally, rectally, ontologically, neuro-otologically, intraocularly, subconjuctivally, via anterior eye chamber injection, intravitreally, intraperitoneally, intrathecally, intracystically, intrapleurally, via wound irrigation, intrabuccally, intra-abdominally, intra-articularly, intra-aurally, intrabronchially, intracapsularly, intrameningeally, via inhalation, via endotracheal or endobronchial instillation, via direct instillation into pulmonary cavities, intraspinally, intrasynovially, intrathoracically, via thoracostomy irrigation, epidurally, intratympanically, intracisternally, intravascularly, intraventricularly, intraosseously, via irrigation of infected bone, or via application as part of any admixture with a prosthetic devices. In some embodiments, the administration method includes oral or parenteral administration.

Compounds provided herein intended for pharmaceutical use may be administered as crystalline or amorphous products. Pharmaceutically acceptable compositions may include solid, semi-solid, liquid, solutions, colloidal, liposomes, emulsions, suspensions, complexes, coacervates and aerosols. Dosage forms, such as, e.g., tablets, capsules, powders, liquids, suspensions, suppositories, aerosols, implants, controlled release or the like. They may be obtained, for example, as solid plugs, powders, or films by methods such as precipitation, crystallization, milling, grinding, supercritical fluid processing, coacervation, complex coacervation, encapsulation, emulsification, complexation, freeze drying, spray drying, or evaporative drying. Microwave or radio frequency drying may be used for this purpose. The compounds can also be administered in sustained or controlled release dosage forms, including depot injections, osmotic pumps, pills (tablets and or capsules), transdermal (including electrotransport) patches, implants and the like, for prolonged and/or timed, pulsed administration at a predetermined rate.

The compounds can be administered either alone or in combination with a conventional pharmaceutical carrier, excipient or the like. Pharmaceutically acceptable excipients include, but are not limited to, ion exchangers, alumina, aluminum stearate, lecithin, self-emulsifying drug delivery systems (SEDDS) such as d-α-tocopherol polyethylene glycol 1000 succinate, surfactants used in pharmaceutical dosage forms such as Tweens, poloxamers or other similar polymeric delivery matrices, serum proteins, such as human serum albumin, buffer substances such as phosphates, tris, glycine, sorbic acid, potassium sorbate, partial glyceride mixtures of saturated vegetable fatty acids, water, salts or electrolytes, such as protamine sulfate, disodium hydrogen phosphate, potassium hydrogen phosphate, sodium-chloride, zinc salts, colloidal silica, magnesium trisilicate, polyvinyl pyrrolidone, cellulose-based substances, polyethylene glycol, sodium carboxymethyl cellulose, polyacrylates, waxes, polyethylene-polyoxypropylene-block polymers, and wool fat. Cyclodextrins such as α-, β, and γ-cyclodextrin, or chemically modified derivatives such as hydroxyalkylcyclodextrins, including 2- and 3-hydroxypropyl-β-cyclodextrins, or other solubilized derivatives can also be used to enhance delivery of compounds described herein. Dosage forms or compositions containing a compound as described herein in the range of 0.005% to 100% with the balance made up from non-toxic carrier may be prepared. The contemplated compositions may contain 0.001%-100% of a compound provided herein, in one embodiment 0.1-95%, in another embodiment 75-85%, in a further embodiment 20-80%. Actual methods of preparing such dosage forms are known, or will be apparent, to those skilled in this art; for example, see Remington: The Science and Practice of Pharmacy, 22^nd Edition (Pharmaceutical Press, London, UK. 2012).

In one embodiment, the compositions will take the form of a unit dosage form such as a pill or tablet and thus the composition may contain, along with a compound provided herein, a diluent such as lactose, sucrose, dicalcium phosphate, or the like; a lubricant such as magnesium stearate or the like; and a binder such as starch, gum acacia, polyvinylpyrrolidine, gelatin, cellulose, cellulose derivatives or the like. In another solid dosage form, a powder, marume, solution or suspension (e.g., in propylene carbonate, vegetable oils, PEG's, poloxamer 124 or triglycerides) is encapsulated in a capsule (gelatin or cellulose base capsule). Unit dosage forms in which one or more compounds provided herein or additional active agents are physically separated are also contemplated; e.g., capsules with granules (or tablets in a capsule) of each drug; two-layer tablets; two-compartment gel caps, etc. Enteric coated or delayed release oral dosage forms are also contemplated.

Liquid pharmaceutically administrable compositions can, for example, be prepared by dissolving, dispersing, etc. a compound provided herein and optional pharmaceutical adjuvants in a carrier (e.g., water, saline, aqueous dextrose, glycerol, glycols, ethanol or the like) to form a solution, colloid, liposome, emulsion, complexes, coacervate or suspension. If desired, the pharmaceutical composition can also contain minor amounts of nontoxic auxiliary substances such as wetting agents, emulsifying agents, co-solvents, solubilizing agents, pH buffering agents and the like (e.g., sodium acetate, sodium citrate, cyclodextrin derivatives, sorbitan monolaurate, triethanolamine acetate, triethanolamine oleate, and the like).

In some embodiments, the unit dosage of compounds of Formula (I) is about 0.25 mg/Kg to about 50 mg/Kg in humans.

In some embodiments, the unit dosage of compounds of Formula (I) is about 0.25 mg/Kg to about 20 mg/Kg in humans.

In some embodiments, the unit dosage of compounds of Formula (I) is about 0.50 mg/Kg to about 19 mg/Kg in humans.

In some embodiments, the unit dosage of compounds of Formula (I) is about 0.75 mg/Kg to about 18 mg/Kg in humans.

In some embodiments, the unit dosage of compounds of Formula (I) is about 1.0 mg/Kg to about 17 mg/Kg in humans.

In some embodiments, the unit dosage of compounds of Formula (I) is about 1.25 mg/Kg to about 16 mg/Kg in humans.

In some embodiments, the unit dosage of compounds of Formula (I) is about 1.50 mg/Kg to about 15 mg/Kg in humans.

In some embodiments, the unit dosage of compounds of Formula (I) is about 1.75 mg/Kg to about 14 mg/Kg in humans.

In some embodiments, the unit dosage of compounds of Formula (I) is about 2.0 mg/Kg to about 13 mg/Kg in humans.

In some embodiments, the unit dosage of compounds of Formula (I) is about 3.0 mg/Kg to about 12 mg/Kg in humans.

In some embodiments, the unit dosage of compounds of Formula (I) is about 4.0 mg/Kg to about 11 mg/Kg in humans.

In some embodiments, the unit dosage of compounds of Formula (I) is about 5.0 mg/Kg to about 10 mg/Kg in humans.

In some embodiments, the compositions are provided in unit dosage forms suitable for single administration.

In some embodiments, the compositions are provided in unit dosage forms suitable for twice a day administration.

In some embodiments, the compositions are provided in unit dosage forms suitable for three times a day administration.

Injectables can be prepared in conventional forms, either as liquid solutions, colloid, liposomes, complexes, coacervate or suspensions, as emulsions, or in solid forms suitable for reconstitution in liquid prior to injection. The percentage of a compound provided herein contained in such parenteral compositions is highly dependent on the specific nature thereof, as well as the activity of the compound and the needs of the patient. However, percentages of active ingredient of 0.01% to 10% in solution are employable, and could be higher if the composition is a solid or suspension, which could be subsequently diluted to the above percentages.

In some embodiments, the composition will comprise about 0.1-10% of the active agent in solution.

In some embodiments, the composition will comprise about 0.1-5% of the active agent in solution.

In some embodiments, the composition will comprise about 0.1-4% of the active agent in solution.

In some embodiments, the composition will comprise about 0.15-3% of the active agent in solution.

In some embodiments, the composition will comprise about 0.2-2% of the active agent in solution.

In some embodiments, the compositions are provided in dosage forms suitable for continuous dosage by intravenous infusion over a period of about 1-96 hours.

In some embodiments, the compositions are provided in dosage forms suitable for continuous dosage by intravenous infusion over a period of about 1-72 hours.

In some embodiments, the compositions are provided in dosage forms suitable for continuous dosage by intravenous infusion over a period of about 1-48 hours.

In some embodiments, the compositions are provided in dosage forms suitable for continuous dosage by intravenous infusion over a period of about 1-24 hours.

In some embodiments, the compositions are provided in dosage forms suitable for continuous dosage by intravenous infusion over a period of about 1-12 hours.

In some embodiments, the compositions are provided in dosage forms suitable for continuous dosage by intravenous infusion over a period of about 1-6 hours.

In some embodiments, these compositions can be administered by intravenous infusion to humans at doses of about 5 mg/m² to about 300 mg/m².

In some embodiments, these compositions can be administered by intravenous infusion to humans at doses of about 5 mg/m² to about 200 mg/m².

In some embodiments, these compositions can be administered by intravenous infusion to humans at doses of about 5 mg/m² to about 100 mg/m².

In some embodiments, these compositions can be administered by intravenous infusion to humans at doses of about 10 mg/m² to about 50 mg/m².

In some embodiments, these compositions can be administered by intravenous infusion to humans at doses of about 50 mg/m² to about 200 mg/m².

In some embodiments, these compositions can be administered by intravenous infusion to humans at doses of about 75 mg/m² to about 175 mg/m².

In some embodiments, these compositions can be administered by intravenous infusion to humans at doses of about 100 mg/m² to about 150 mg/m².

It is to be noted that concentrations and dosage values may also vary depending on the specific compound and the severity of the condition to be alleviated. It is to be further understood that for any particular patient, specific dosage regimens should be adjusted over time according to the individual need and the professional judgment of the person administering or supervising the administration of the compositions, and that the concentration ranges set forth herein are exemplary only and are not intended to limit the scope or practice of the claimed compositions.

In one embodiment, the compositions can be administered to the respiratory tract (including nasal and pulmonary) e.g., through a nebulizer, metered-dose inhalers, atomizer, mister, aerosol, dry powder inhaler, insufflator, liquid instillation or other suitable device or technique.

In some embodiments, aerosols intended for delivery to the nasal mucosa are provided for inhalation through the nose. For optimal delivery to the nasal cavities, inhaled particle sizes of about 5 to about 100 microns are useful, with particle sizes of about 10 to about 60 microns being preferred. For nasal delivery, a larger inhaled particle size may be desired to maximize impaction on the nasal mucosa and to minimize or prevent pulmonary deposition of the administered formulation. In some embodiments, aerosols intended for delivery to the lung are provided for inhalation through the nose or the mouth. For delivery to the lung, inhaled aerodynamic particle sizes of about less than 10 μm are useful (e.g., about 1 to about 10 microns). Inhaled particles may be defined as liquid droplets containing dissolved drug, liquid droplets containing suspended drug particles (in cases where the drug is insoluble in the suspending medium), dry particles of pure drug substance, drug substance incorporated with excipients, liposomes, emulsions, colloidal systems, coacervates, aggregates of drug nanoparticles, or dry particles of a diluent which contain embedded drug nanoparticles.

In some embodiments, compounds of Formula (I) disclosed herein intended for respiratory delivery (either systemic or local) can be administered as aqueous formulations, as non-aqueous solutions or suspensions, as suspensions or solutions in halogenated hydrocarbon propellants with or without alcohol, as a colloidal system, as emulsions, coacervates, or as dry powders. Aqueous formulations may be aerosolized by liquid nebulizers employing either hydraulic or ultrasonic atomization or by modified micropump systems (like the soft mist inhalers, the Aerodose® or the AERx® systems). Propellant-based systems may use suitable pressurized metered-dose inhalers (pMDIs). Dry powders may use dry powder inhaler devices (DPIs), which are capable of dispersing the drug substance effectively. A desired particle size and distribution may be obtained by choosing an appropriate device.

In some embodiments, the compositions of Formula (I) disclosed herein can be administered to the ear by various methods. For example, a round window catheter (e.g., U.S. Pat. Nos. 6,440,102 and 6,648,873) can be used.

Alternatively, formulations can be incorporated into a wick for use between the outer and middle ear (e.g., U.S. Pat. No. 6,120,484) or absorbed to collagen sponge or other solid support (e.g., U.S. Pat. No. 4,164,559).

If desired, formulations of the invention can be incorporated into a gel formulation (e.g., U.S. Pat. Nos. 4,474,752 and 6,911,211).

In some embodiments, compounds of Formula (I) disclosed herein intended for delivery to the ear can be administered via an implanted pump and delivery system through a needle directly into the middle or inner ear (cochlea) or through a cochlear implant stylet electrode channel or alternative prepared drug delivery channel such as but not limited to a needle through temporal bone into the cochlea.

Other options include delivery via a pump through a thin film coated onto a multichannel electrode or electrode with a specially imbedded drug delivery channel (pathways) carved into the thin film for this purpose. In other embodiments the acidic or basic solid compound of Formula (I) can be delivered from the reservoir of an external or internal implanted pumping system.

Formulations of the invention also can be administered to the ear by intratympanic injection into the middle ear, inner ear, or cochlea (e.g., U.S. Pat. No. 6,377,849 and Ser. No. 11/337,815).

Intratympanic injection of therapeutic agents is the technique of injecting a therapeutic agent behind the tympanic membrane into the middle and/or inner ear. In one embodiment, the formulations described herein are administered directly onto the round window membrane via transtympanic injection. In another embodiment, the ion channel modulating agent auris-acceptable formulations described herein are administered onto the round window membrane via a non-transtympanic approach to the inner ear. In additional embodiments, the formulation described herein is administered onto the round window membrane via a surgical approach to the round window membrane comprising modification of the crista fenestrae cochleae.

In some embodiments, the compounds of Formula (I) are formulated in rectal compositions such as enemas, rectal gels, rectal foams, rectal aerosols, suppositories, jelly suppositories, or retention enemas, containing conventional suppository bases such as cocoa butter or other glycerides, as well as synthetic polymers such as polyvinylpyrrolidone, PEG (like PEG ointments), and the like.

Suppositories for rectal administration of the drug (either as a solution, colloid, suspension or a complex) can be prepared by mixing a compound provided herein with a suitable non-irritating excipient that is solid at ordinary temperatures but liquid at the rectal temperature and will therefore melt or erode/dissolve in the rectum and release the compound. Such materials include cocoa butter, glycerinated gelatin, hydrogenated vegetable oils, poloxamers, mixtures of polyethylene glycols of various molecular weights and fatty acid esters of polyethylene glycol. In suppository forms of the compositions, a low-melting wax such as, but not limited to, a mixture of fatty acid glycerides, optionally in combination with cocoa butter, is first melted.

Solid compositions can be provided in various different types of dosage forms, depending on the physicochemical properties of the compound provided herein, the desired dissolution rate, cost considerations, and other criteria. In one of the embodiments, the solid composition is a single unit. This implies that one unit dose of the compound is comprised in a single, physically shaped solid form or article. In other words, the solid composition is coherent, which is in contrast to a multiple unit dosage form, in which the units are incoherent.

Examples of single units which may be used as dosage forms for the solid composition include tablets, such as compressed tablets, film-like units, foil-like units, wafers, lyophilized matrix units, and the like. In one embodiment, the solid composition is a highly porous lyophilized form. Such lyophilizates, sometimes also called wafers or lyophilized tablets, are particularly useful for their rapid disintegration, which also enables the rapid dissolution of the compound.

On the other hand, for some applications the solid composition may also be formed as a multiple unit dosage form as defined above. Examples of multiple units are powders, granules, microparticles, pellets, mini-tablets, beads, lyophilized powders, and the like. In one embodiment, the solid composition is a lyophilized powder. Such a dispersed lyophilized system comprises a multitude of powder particles, and due to the lyophilization process used in the formation of the powder, each particle has an irregular, porous microstructure through which the powder is capable of absorbing water very rapidly, resulting in quick dissolution. Effervescent compositions are also contemplated to aid the quick dispersion and absorption of the compound.

Another type of multiparticulate system which is also capable of achieving rapid drug dissolution is that of powders, granules, or pellets from water-soluble excipients which are coated with a compound provided herein so that the compound is located at the outer surface of the individual particles. In this type of system, the water-soluble low molecular weight excipient may be useful for preparing the cores of such coated particles, which can be subsequently coated with a coating composition comprising the compound and, for example, one or more additional excipients, such as a binder, a pore former, a saccharide, a sugar alcohol, a film-forming polymer, a plasticizer, or other excipients used in pharmaceutical coating compositions.

Also provided herein are kits. Typically, a kit includes one or more compounds or compositions as described herein. In certain embodiments, a kit can include one or more delivery systems, e.g., for delivering or administering a compound as provided herein, and directions for use of the kit (e.g., instructions for treating a patient). In another embodiment, the kit can include a compound or composition as described herein and a label that indicates that the contents are to be administered to a patient with cancer. In another embodiment, the kit can include a compound or composition as described herein and a label that indicates that the contents are to be administered to a patient with one or more of hepatocellular carcinoma, colon cancer, leukemia, lymphoma, sarcoma, ovarian cancer, diabetic retinopathy, pulmonary fibrosis, rheumatoid arthritis, sepsis, anklyosing spondylitis, psoriasis, scleroderma, mycotic and viral infections, bone and cartilage diseases, Alzheimer's disease, lung disease, bone/osteoporotic (wrist, spine, shoulder and hip) fractures, articular cartilage (chondral) defects, degenerative disc disease (or intervertebral disc degeneration), polyposis coli, bone density and vascular defects in the eye (Osteoporosis-pseudoglioma Syndrome, OPPG) and other eye diseases or syndromes associated with defects and/or damaged photoreceptors, familial exudative vitreoretinopathy, retinal angiogenesis, early coronary disease, tetra-amelia, Müllerian-duct regression and virilization, SERKAL syndrome, type II diabetes, Fuhrmann syndrome, Al-Awadi/Raas-Rothschild/Schinzel phocomelia syndrome, odonto-onycho-dermal dysplasia, obesity, split-hand/foot malformation, caudal duplication, tooth agenesis, Wilms tumor, skeletal dysplasia, focal dermal hypoplasia, autosomal recessive anonychia, neural tube defects, alpha-thalassemia (ATRX) syndrome, fragile X syndrome, ICF syndrome, Angelman's syndrome, Prader-Willi syndrome, Beckwith-Wiedemann Syndrome, Norrie disease, and Rett syndrome.

Methods of Treatment

The compounds and compositions provided herein can be used as inhibitors and/or modulators of one or more components of the Wnt pathway, which may include one or more Wnt proteins, and thus can be used to treat a variety of disorders and diseases in which aberrant Wnt signaling is implicated, such as cancer and other diseases associated with abnormal angiogenesis, cellular proliferation, and cell cycling. Accordingly, the compounds and compositions provided herein can be used to treat cancer, to reduce or inhibit angiogenesis, to reduce or inhibit cellular proliferation, to correct a genetic disorder, and/or to treat a neurological condition/disorder/disease due to mutations or dysregulation of the Wnt pathway and/or of one or more of Wnt signaling components. Non-limiting examples of diseases which can be treated with the compounds and compositions provided herein include a variety of cancers, diabetic retinopathy, pulmonary fibrosis, rheumatoid arthritis, scleroderma, mycotic and viral infections, bone and cartilage diseases, neurological conditions/diseases such as Alzheimer's disease, amyotrophic lateral sclerosis (ALS), motor neuron disease, multiple sclerosis or autism, lung disease, bone/osteoporotic (wrist, spine, shoulder and hip) fractures, polyposis coli, bone density and vascular defects in the eye (Osteoporosis-pseudoglioma Syndrome, OPPG) and other eye diseases or syndromes associated with defects and/or damaged photoreceptors, familial exudative vitreoretinopathy, retinal angiogenesis, early coronary disease, tetra-amelia, Müllerian-duct regression and virilization, SERKAL syndrome, type II diabetes, Fuhrmann syndrome, Al-Awadi/Raas-Rothschild/Schinzel phocomelia syndrome, odonto-onycho-dermal dysplasia, obesity, split-hand/foot malformation, caudal duplication, tooth agenesis, Wilms tumor, skeletal dysplasia, focal dermal hypoplasia, autosomal recessive anonychia, neural tube defects, alpha-thalassemia (ATRX) syndrome, fragile X syndrome, ICF syndrome, Angelman's syndrome, Prader-Willi syndrome, Beckwith-Wiedemann Syndrome, Norrie disease and Rett syndrome.

In some embodiments, non-limiting examples of eye diseases which can be treated with the compounds and compositions provided herein include age-related macular degeneration (AMD or ARMD), rod cone dystrophy, retinitis pigmentosa (RP), acute idiopathic blind spot enlargement (AIBSE), acute zonal occult outer retinopathy (AZOOR), acute macular neuroretinopathy (AMN), multiple evanescent white dot syndrome (MEWDS), multifocal choroiditis, opticneuropathy. Further causes of photoreceptor damage that can be treated with the compounds and compositions provided herein include retinal detachment, vascular disturbance, eye tumors or extreme light damage.

With respect to cancer, the Wnt pathway is known to be constitutively activated in a variety of cancers including, for example, colon cancer, hepatocellular carcinoma, lung cancer, ovarian cancer, prostate cancer, pancreatic cancer and leukemias such as CML, CLL and T-ALL. Accordingly, the compounds and compositions described herein may be used to treat these cancers in which the Wnt pathway is constitutively activated. In certain embodiments, the cancer is chosen from hepatocellular carcinoma, colon cancer, leukemia, lymphoma, sarcoma and ovarian cancer.

Other cancers can also be treated with the compounds and compositions described herein.

More particularly, cancers that may be treated by the compounds, compositions and methods described herein include, but are not limited to, the following:

1) Breast cancers, including, for example ER⁺ breast cancer, ER⁻ breast cancer, her2⁻ breast cancer, her2⁺ breast cancer, stromal tumors such as fibroadenomas, phyllodes tumors, and sarcomas, and epithelial tumors such as large duct papillomas; carcinomas of the breast including in situ (noninvasive) carcinoma that includes ductal carcinoma in situ (including Paget's disease) and lobular carcinoma in situ, and invasive (infiltrating) carcinoma including, but not limited to, invasive ductal carcinoma, invasive lobular carcinoma, medullary carcinoma, colloid (mucinous) carcinoma, tubular carcinoma, and invasive papillary carcinoma; and miscellaneous malignant neoplasms. Further examples of breast cancers can include luminal A, luminal B, basal A, basal B, and triple negative breast cancer, which is estrogen receptor negative (ER⁻), progesterone receptor negative, and her2 negative (her2⁻). In some embodiments, the breast cancer may have a high risk Oncotype score.

2) Cardiac cancers, including, for example sarcoma, e.g., angiosarcoma, fibrosarcoma, rhabdomyosarcoma, and liposarcoma; myxoma; rhabdomyoma; fibroma; lipoma and teratoma.

3) Lung cancers, including, for example, bronchogenic carcinoma, e.g., squamous cell, undifferentiated small cell, undifferentiated large cell, and adenocarcinoma; alveolar and bronchiolar carcinoma; bronchial adenoma; sarcoma; lymphoma; chondromatous hamartoma; and mesothelioma.

4) Gastrointestinal cancer, including, for example, cancers of the esophagus, e.g., squamous cell carcinoma, adenocarcinoma, leiomyosarcoma, and lymphoma; cancers of the stomach, e.g., carcinoma, lymphoma, and leiomyosarcoma; cancers of the pancreas, e.g., ductal adenocarcinoma, insulinoma, glucagonoma, gastrinoma, carcinoid tumors, and vipoma; cancers of the small bowel, e.g., adenocarcinoma, lymphoma, carcinoid tumors, Kaposi's sarcoma, leiomyoma, hemangioma, lipoma, neurofibroma, and fibroma; cancers of the large bowel, e.g., adenocarcinoma, tubular adenoma, villous adenoma, hamartoma, and leiomyoma.

5) Genitourinary tract cancers, including, for example, cancers of the kidney, e.g., adenocarcinoma, Wilm's tumor (nephroblastoma), lymphoma, and leukemia; cancers of the bladder and urethra, e.g., squamous cell carcinoma, transitional cell carcinoma, and adenocarcinoma; cancers of the prostate, e.g., adenocarcinoma, and sarcoma; cancer of the testis, e.g., seminoma, teratoma, embryonal carcinoma, teratocarcinoma, choriocarcinoma, sarcoma, interstitial cell carcinoma, fibroma, fibroadenoma, adenomatoid tumors, and lipoma.

6) Liver cancers, including, for example, hepatoma, e.g., hepatocellular carcinoma; cholangiocarcinoma; hepatoblastoma; angiosarcoma; hepatocellular adenoma; and hemangioma.

7) Bone cancers, including, for example, osteogenic sarcoma (osteosarcoma), fibrosarcoma, malignant fibrous histiocytoma, chondrosarcoma, Ewing's sarcoma, malignant lymphoma (reticulum cell sarcoma), multiple myeloma, malignant giant cell tumor chordoma, osteochrondroma (osteocartilaginous exostoses), benign chondroma, chondroblastoma, chondromyxofibroma, osteoid osteoma and giant cell tumors.

8) Nervous system cancers, including, for example, cancers of the skull, e.g., osteoma, hemangioma, granuloma, xanthoma, and osteitis deformans; cancers of the meninges, e.g., meningioma, meningiosarcoma, and gliomatosis; cancers of the brain, e.g., astrocytoma, medulloblastoma, glioma, ependymoma, germinoma (pinealoma), glioblastoma multiform, oligodendroglioma, schwannoma, retinoblastoma, and congenital tumors; and cancers of the spinal cord, e.g., neurofibroma, meningioma, glioma, and sarcoma.

9) Gynecological cancers, including, for example, cancers of the uterus, e.g., endometrial carcinoma; cancers of the cervix, e.g., cervical carcinoma, and pre tumor cervical dysplasia; cancers of the ovaries, e.g., ovarian carcinoma, including serous cystadenocarcinoma, mucinous cystadenocarcinoma, unclassified carcinoma, granulosa theca cell tumors, Sertoli Leydig cell tumors, dysgerminoma, and malignant teratoma; cancers of the vulva, e.g., squamous cell carcinoma, intraepithelial carcinoma, adenocarcinoma, fibrosarcoma, and melanoma; cancers of the vagina, e.g., clear cell carcinoma, squamous cell carcinoma, botryoid sarcoma, and embryonal rhabdomyosarcoma; and cancers of the fallopian tubes, e.g., carcinoma.

10) Hematologic cancers, including, for example, cancers of the blood, e.g., acute myeloid leukemia, chronic myeloid leukemia, acute lymphoblastic leukemia, chronic lymphocytic leukemia, myeloproliferative diseases, multiple myeloma, and myelodysplastic syndrome, Hodgkin's lymphoma, non-Hodgkin's lymphoma (malignant lymphoma) and Waldenström's macroglobulinemia.

11) Skin cancers and skin disorders, including, for example, malignant melanoma and metastatic melanoma, basal cell carcinoma, squamous cell carcinoma, Kaposi's sarcoma, moles dysplastic nevi, lipoma, angioma, dermatofibroma, keloids, and scleroderma.

12) Adrenal gland cancers, including, for example, neuroblastoma.

Cancers may be solid tumors that may or may not be metastatic. Cancers may also occur, as in leukemia, as a diffuse tissue. Thus, the term “tumor cell,” as provided herein, includes a cell afflicted by any one of the above identified disorders.

A method of treating cancer using a compound or composition as described herein may be combined with existing methods of treating cancers, for example by chemotherapy, irradiation, or surgery (e.g., oophorectomy). In some embodiments, a compound or composition can be administered before, during, or after another anticancer agent or treatment.

The compounds and compositions described herein can be used as anti-angiogenesis agents and as agents for modulating and/or inhibiting the activity of protein kinases, thus providing treatments for cancer and other diseases associated with cellular proliferation mediated by protein kinases. For example, the compounds described herein can inhibit the activity of one or more kinases. Accordingly, provided herein is a method of treating cancer or preventing or reducing angiogenesis through kinase inhibition.

In addition, and including treatment of cancer, the compounds and compositions described herein can function as cell-cycle control agents for treating proliferative disorders in a patient. Disorders associated with excessive proliferation include, for example, cancers, scleroderma, immunological disorders involving undesired proliferation of leukocytes, and restenosis and other smooth muscle disorders. Furthermore, such compounds may be used to prevent de-differentiation of post-mitotic tissue and/or cells.

Diseases or disorders associated with uncontrolled or abnormal cellular proliferation include, but are not limited to, the following:

• • a variety of cancers, including, but not limited to, carcinoma, hematopoietic tumors of lymphoid lineage, hematopoietic tumors of myeloid lineage, tumors of mesenchymal origin, tumors of the central and peripheral nervous system and other tumors including melanoma, seminoma and Kaposi's sarcoma.
  • a disease process which features abnormal cellular proliferation, e.g., benign prostatic hyperplasia, familial adenomatosis polyposis, neurofibromatosis, atherosclerosis, arthritis, glomerulonephritis, restenosis following angioplasty or vascular surgery, inflammatory bowel disease, transplantation rejection, endotoxic shock, and fungal infections. Fibrotic disorders such as skin fibrosis; scleroderma; progressive systemic fibrosis; lung fibrosis; muscle fibrosis; kidney fibrosis; glomerulosclerosis; glomerulonephritis; hypertrophic scar formation; uterine fibrosis; renal fibrosis; cirrhosis of the liver, liver fibrosis; fatty liver disease (FLD); adhesions, such as those occurring in the abdomen, pelvis, spine or tendons; chronic obstructive pulmonary disease; fibrosis following myocardial infarction; pulmonary fibrosis; fibrosis and scarring associated with diffuse/interstitial lung disease; central nervous system fibrosis, such as fibrosis following stroke; fibrosis associated with neuro-degenerative disorders such as Alzheimer's Disease or multiple sclerosis; fibrosis associated with proliferative vitreoretinopathy (PVR); restenosis; endometriosis; ischemic disease and radiation fibrosis.
  • defective apoptosis-associated conditions, such as cancers (including but not limited to those types mentioned herein), viral infections (including but not limited to herpesvirus, poxvirus, Epstein-Barr virus, Sindbis virus and adenovirus), prevention of AIDS development in HIV-infected individuals, autoimmune diseases (including but not limited to systemic lupus erythematosus, rheumatoid arthritis, sepsis, anklyosing spondylitis, psoriasis, scleroderma, autoimmune mediated glomerulonephritis, inflammatory bowel disease and autoimmune diabetes mellitus), neuro-degenerative disorders (including but not limited to Alzheimer's disease, lung disease, amyotrophic lateral sclerosis, retinitis pigmentosa, Parkinson's disease, AIDS-related dementia, spinal muscular atrophy and cerebellar degeneration), myelodysplastic syndromes, aplastic anemia, ischemic injury associated with myocardial infarctions, stroke and reperfusion injury, arrhythmia, atherosclerosis, toxin-induced or alcohol related liver diseases, hematological diseases (including but not limited to chronic anemia and aplastic anemia), degenerative diseases of the musculoskeletal system (including but not limited to osteoporosis and arthritis), tendinopathies such as tendinitis and tendinosis, aspirin-sensitive rhinosinusitis, cystic fibrosis, multiple sclerosis, kidney diseases and cancer pain.
  • genetic diseases due to mutations in Wnt signaling components, such as polyposis coli, bone density and vascular defects in the eye (Osteoporosis-pseudoglioma Syndrome, OPPG) and other eye diseases or syndromes associated with defects and/or damaged photoreceptors, familial exudative vitreoretinopathy, retinal angiogenesis, early coronary disease, tetra-amelia, Müllerian-duct regression and virilization, SERKAL syndrome, type II diabetes, Fuhrmann syndrome, Al-Awadi/Raas-Rothschild/Schinzel phocomelia syndrome, odonto-onycho-dermal dysplasia, obesity, split-hand/foot malformation, caudal duplication, tooth agenesis, Wilms tumor, skeletal dysplasia, focal dermal hypoplasia, autosomal recessive anonychia, neural tube defects, alpha-thalassemia (ATRX) syndrome, fragile X syndrome, ICF syndrome, Angelman's syndrome, Prader-Willi syndrome, Beckwith-Wiedemann Syndrome, Norrie disease and Rett syndrome.

The compounds and compositions described herein can be used to treat neurological conditions, disorders and/or diseases caused by dysfunction in the Wnt signaling pathway. Non-limiting examples of neurological conditions/disorders/diseases which can be treated with the compounds and compositions provided herein include Alzheimer's disease, aphasia, apraxia, arachnoiditis, ataxia telangiectasia, attention deficit hyperactivity disorder, auditory processing disorder, autism, alcoholism, Bell's palsy, bipolar disorder, brachial plexus injury, Canavan disease, carpal tunnel syndrome, causalgia, central pain syndrome, central pontine myelinolysis, centronuclear myopathy, cephalic disorder, cerebral aneurysm, cerebral arteriosclerosis, cerebral atrophy, cerebral gigantism, cerebral palsy, cerebral vasculitis, cervical spinal stenosis, Charcot-Marie-Tooth disease, Chiari malformation, chronic fatigue syndrome, chronic inflammatory demyelinating polyneuropathy (CIDP), chronic pain, Coffin-Lowry syndrome, complex regional pain syndrome, compression neuropathy, congenital facial diplegia, corticobasal degeneration, cranial arteritis, craniosynostosis, Creutzfeldt-Jakob disease, cumulative trauma disorder, Cushing's syndrome, cytomegalic inclusion body disease (CIBD), Dandy-Walker syndrome, Dawson disease, de Morsier's syndrome, Dejerine-Klumpke palsy, Dejerine-Sottas disease, delayed sleep phase syndrome, dementia, dermatomyositis, developmental dyspraxia, diabetic neuropathy, diffuse sclerosis, Dravet syndrome, dysautonomia, dyscalculia, dysgraphia, dyslexia, dystonia, empty sella syndrome, encephalitis, encephalocele, encephalotrigeminal angiomatosis, encopresis, epilepsy, Erb's palsy, erythromelalgia, essential tremor, Fabry's disease, Fahr's syndrome, familial spastic paralysis, febrile seizure, Fisher syndrome, Friedreich's ataxia, fibromyalgia, Foville's syndrome, Gaucher's disease, Gerstmann's syndrome, giant cell arteritis, giant cell inclusion disease, globoid cell leukodystrophy, gray matter heterotopia, Guillain-Barré syndrome, HTLV-1 associated myelopathy, Hallervorden-Spatz disease, hemifacial spasm, hereditary spastic paraplegia, heredopathia atactica polyneuritiformis, herpes zoster oticus, herpes zoster, Hirayama syndrome, holoprosencephaly, Huntington's disease, hydranencephaly, hydrocephalus, hypercortisolism, hypoxia, immune-mediated encephalomyelitis, inclusion body myositis, incontinentia pigmenti, infantile phytanic acid storage disease, infantile Refsum disease, infantile spasms, inflammatory myopathy, intracranial cyst, intracranial hypertension, Joubert syndrome, Karak syndrome, Kearns-Sayre syndrome, Kennedy disease, Kinsbourne syndrome, Klippel Feil syndrome, Krabbe disease, Kugelberg-Welander disease, kuru, Lafora disease, Lambert-Eaton myasthenic syndrome, Landau-Kleffner syndrome, lateral medullary (Wallenberg) syndrome, Leigh's disease, Lennox-Gastaut syndrome, Lesch-Nyhan syndrome, leukodystrophy, Lewy body dementia, lissencephaly, locked-in syndrome, Lou Gehrig's disease, lumbar disc disease, lumbar spinal stenosis, Lyme disease, Machado-Joseph disease (Spinocerebellar ataxia type 3), macrencephaly, macropsia, megalencephaly, Melkersson-Rosenthal syndrome, Meniere's disease, meningitis, Menkes disease, metachromatic leukodystrophy, microcephaly, micropsia, Miller Fisher syndrome, misophonia, mitochondrial myopathy, Mobius syndrome, monomelic amyotrophy, motor neuron disease, motor skills disorder, Moyamoya disease, mucopolysaccharidoses, multi-infarct dementia, multifocal motor neuropathy, multiple sclerosis, multiple system atrophy, muscular dystrophy, myalgic encephalomyelitis, myasthenia gravis, myelinoclastic diffuse sclerosis, myoclonic Encephalopathy of infants, myoclonus, myopathy, myotubular myopathy, myotonia congenital, narcolepsy, neurofibromatosis, neuroleptic malignant syndrome, lupus erythematosus, neuromyotonia, neuronal ceroid lipofuscinosis, Niemann-Pick disease, O'Sullivan-McLeod syndrome, occipital Neuralgia, occult Spinal Dysraphism Sequence, Ohtahara syndrome, olivopontocerebellar atrophy, opsoclonus myoclonus syndrome, optic neuritis, orthostatic hypotension, palinopsia, paresthesia, Parkinson's disease, paramyotonia Congenita, paraneoplastic diseases, paroxysmal attacks, Parry-Romberg syndrome, Pelizaeus-Merzbacher disease, periodic paralyses, peripheral neuropathy, photic sneeze reflex, phytanic acid storage disease, Pick's disease, polymicrogyria (PMG), polymyositis, porencephaly, post-polio syndrome, postherpetic neuralgia (PHN), postural hypotension, Prader-Willi syndrome, primary lateral sclerosis, prion diseases, progressive hemifacial atrophy, progressive multifocal leukoencephalopathy, progressive supranuclear palsy, pseudotumor cerebri, Ramsay Hunt syndrome type I, Ramsay Hunt syndrome type II, Ramsay Hunt syndrome type III, Rasmussen's encephalitis, reflex neurovascular dystrophy, Refsum disease, restless legs syndrome, retrovirus-associated myelopathy, Rett syndrome, Reye's syndrome, rhythmic movement disorder, Romberg syndrome, Saint Vitus dance, Sandhoff disease, schizophrenia, Schilder's disease, schizencephaly, sensory integration dysfunction, septo-optic dysplasia, Shy-Drager syndrome, Sjögren's syndrome, snatiation, Sotos syndrome, spasticity, spina bifida, spinal cord tumors, spinal muscular atrophy, spinocerebellar ataxia, Steele-Richardson-Olszewski syndrome, Stiff-person syndrome, stroke, Sturge-Weber syndrome, subacute sclerosing panencephalitis, subcortical arteriosclerotic encephalopathy, superficial siderosis, Sydenham's chorea, syncope, synesthesia, syringomyelia, tarsal tunnel syndrome, tardive dyskinesia, tardive dysphrenia, Tarlov cyst, Tay-Sachs disease, temporal arteritis, tetanus, tethered spinal cord syndrome, Thomsen disease, thoracic outlet syndrome, tic douloureux, Todd's paralysis, Tourette syndrome, toxic encephalopathy, transient ischemic attack, transmissible spongiform encephalopathies, transverse myelitis, tremor, trigeminal neuralgia, tropical spastic paraparesis, trypanosomiasis, tuberous sclerosis, ubisiosis, Von Hippel-Lindau disease (VHL), Viliuisk Encephalomyelitis (VE), Wallenberg's syndrome, Werdnig, Hoffman disease, west syndrome, Williams syndrome, Wilson's disease and Zellweger syndrome.

The compounds and compositions may also be useful in the inhibition of the development of invasive cancer, tumor angiogenesis and metastasis.

In some embodiments, the disclosure provides a method for treating a disease or disorder associated with aberrant cellular proliferation by administering to a patient in need of such treatment an effective amount of one or more of the compounds of Formula (I), in combination (simultaneously or sequentially) with at least one other agent.

In some embodiments, the disclosure provides a method of treating or ameliorating in a patient a disorder or disease selected from the group consisting of: cancer, pulmonary fibrosis, idiopathic pulmonary fibrosis (IPF), degenerative disc disease, bone/osteoporotic fractures, bone or cartilage disease, and osteoarthritis, the method comprising administering to the patient a therapeutically effective amount of a compound according to claim 1, or a pharmaceutically acceptable salt thereof.

In some embodiments, the pharmaceutical composition comprises a therapeutically effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable excipient.

In some embodiments, the method of treats a disorder or disease in which aberrant Wnt signaling is implicated in a patient, the method comprises administering to the patient a therapeutically effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt thereof.

In some embodiments, the disorder or disease is cancer.

In some embodiments, the disorder or disease is systemic inflammation.

In some embodiments, the disorder or disease is metastatic melanoma.

In some embodiments, the disorder or disease is fatty liver disease.

In some embodiments, the disorder or disease is liver fibrosis.

In some embodiments, the disorder or disease is tendonitis.

In some embodiments, the disorder or disease is damage to a tendon which would benefit from tendon regeneration.

In some embodiments, the disorder or disease is diabetes.

In some embodiments, the disorder or disease is degenerative disc disease.

In some embodiments, the disorder or disease is osteoarthritis.

In some embodiments, the disorder or disease is diabetic retinopathy.

In some embodiments, the disorder or disease is pulmonary fibrosis.

In some embodiments, the disorder or disease is idiopathic pulmonary fibrosis (IPF).

In some embodiments, the disorder or disease is degenerative disc disease.

In some embodiments, the disorder or disease is rheumatoid arthritis.

In some embodiments, the disorder or disease is scleroderma.

In some embodiments, the disorder or disease is a mycotic or viral infection.

In some embodiments, the disorder or disease is a bone or cartilage disease.

In some embodiments, the disorder or disease is Alzheimer's disease.

In some embodiments, the disorder or disease is osteoarthritis.

In some embodiments, the disorder or disease is lung disease

In some embodiments, the disorder or disease is a genetic disease caused by mutations in Wnt signaling components, wherein the genetic disease is selected from: polyposis coli, osteoporosis-pseudoglioma syndrome, familial exudative vitreoretinopathy, retinal angiogenesis, early coronary disease, tetra-amelia syndrome, Müllerian-duct regression and virilization, SERKAL syndrome, diabetes mellitus type 2, Fuhrmann syndrome, Al-Awadi/Raas-Rothschild/Schinzel phocomelia syndrome, odonto-onycho-dermal dysplasia, obesity, split-hand/foot malformation, caudal duplication syndrome, tooth agenesis, Wilms tumor, skeletal dysplasia, focal dermal hypoplasia, autosomal recessive anonychia, neural tube defects, alpha-thalassemia (ATRX) syndrome, fragile X syndrome, ICF syndrome, Angelman syndrome, Prader-Willi syndrome, Beckwith-Wiedemann Syndrome, Norrie disease and Rett syndrome.

In some embodiments, the patient is a human.

In some embodiments, the cancer is chosen from: hepatocellular carcinoma, colon cancer, breast cancer, pancreatic cancer, chronic myeloid leukemia (CML), chronic myelomonocytic leukemia, chronic lymphocytic leukemia (CLL), acute myeloid leukemia, acute lymphocytic leukemia, Hodgkin lymphoma, lymphoma, sarcoma and ovarian cancer.

In some embodiments, the cancer is chosen from: lung cancer—non-small cell, lung cancer—small cell, multiple myeloma, nasopharyngeal cancer, neuroblastoma, osteosarcoma, penile cancer, pituitary tumors, prostate cancer, retinoblastoma, rhabdomyosarcoma, salivary gland cancer, skin cancer—basal and squamous cell, skin cancer—melanoma, small intestine cancer, stomach (gastric) cancers, testicular cancer, thymus cancer, thyroid cancer, uterine sarcoma, vaginal cancer, vulvar cancer, laryngeal or hypopharyngeal cancer, kidney cancer, Kaposi sarcoma, gestational trophoblastic disease, gastrointestinal stromal tumor, gastrointestinal carcinoid tumor, gallbladder cancer, eye cancer (melanoma and lymphoma), Ewing tumor, esophagus cancer, endometrial cancer, colorectal cancer, cervical cancer, brain or spinal cord tumor, bone metastasis, bone cancer, bladder cancer, bile duct cancer, anal cancer and adrenal cortical cancer.

In some embodiments, the cancer is hepatocellular carcinoma.

In some embodiments, the cancer is colon cancer.

In some embodiments, the cancer is colorectal cancer.

In some embodiments, the cancer is breast cancer.

In some embodiments, the cancer is pancreatic cancer.

In some embodiments, the cancer is chronic myeloid leukemia (CML).

In some embodiments, the cancer is chronic myelomonocytic leukemia.

In some embodiments, the cancer is chronic lymphocytic leukemia (CLL).

In some embodiments, the cancer is acute myeloid leukemia.

In some embodiments, the cancer is acute lymphocytic leukemia.

In some embodiments, the cancer is Hodgkin lymphoma.

In some embodiments, the cancer is lymphoma.

In some embodiments, the cancer is sarcoma.

In some embodiments, the cancer is ovarian cancer.

In some embodiments, the cancer is lung cancer—non-small cell.

In some embodiments, the cancer is lung cancer—small cell.

In some embodiments, the cancer is multiple myeloma.

In some embodiments, the cancer is nasopharyngeal cancer.

In some embodiments, the cancer is neuroblastoma.

In some embodiments, the cancer is osteosarcoma.

In some embodiments, the cancer is penile cancer.

In some embodiments, the cancer is pituitary tumors.

In some embodiments, the cancer is prostate cancer.

In some embodiments, the cancer is retinoblastoma.

In some embodiments, the cancer is rhabdomyosarcoma.

In some embodiments, the cancer is salivary gland cancer.

In some embodiments, the cancer is skin cancer—basal and squamous cell.

In some embodiments, the cancer is skin cancer—melanoma.

In some embodiments, the cancer is small intestine cancer.

In some embodiments, the cancer is stomach (gastric) cancers.

In some embodiments, the cancer is testicular cancer.

In some embodiments, the cancer is thymus cancer.

In some embodiments, the cancer is thyroid cancer.

In some embodiments, the cancer is uterine sarcoma.

In some embodiments, the cancer is vaginal cancer.

In some embodiments, the cancer is vulvar cancer.

In some embodiments, the cancer is Wilms tumor.

In some embodiments, the cancer is laryngeal or hypopharyngeal cancer.

In some embodiments, the cancer is kidney cancer.

In some embodiments, the cancer is Kaposi sarcoma.

In some embodiments, the cancer is gestational trophoblastic disease.

In some embodiments, the cancer is gastrointestinal stromal tumor.

In some embodiments, the cancer is gastrointestinal carcinoid tumor.

In some embodiments, the cancer is gallbladder cancer.

In some embodiments, the cancer is eye cancer (melanoma and lymphoma).

In some embodiments, the cancer is Ewing tumor.

In some embodiments, the cancer is esophagus cancer.

In some embodiments, the cancer is endometrial cancer.

In some embodiments, the cancer is colorectal cancer.

In some embodiments, the cancer is cervical cancer.

In some embodiments, the cancer is brain or spinal cord tumor.

In some embodiments, the cancer is bone metastasis.

In some embodiments, the cancer is bone cancer.

In some embodiments, the cancer is bladder cancer.

In some embodiments, the cancer is bile duct cancer.

In some embodiments, the cancer is anal cancer.

In some embodiments, the cancer is adrenal cortical cancer.

In some embodiments, the disorder or disease is a neurological condition, disorder or disease, wherein the neurological condition/disorder/disease is selected from: Alzheimer's disease, frontotemporal dementias, dementia with lewy bodies, prion diseases, Parkinson's disease, Huntington's disease, progressive supranuclear palsy, corticobasal degeneration, multiple system atrophy, amyotrophic lateral sclerosis (ALS), inclusion body myositis, autism, degenerative myopathies, diabetic neuropathy, other metabolic neuropathies, endocrine neuropathies, orthostatic hypotension, multiple sclerosis and Charcot-Marie-Tooth disease.

In some embodiments, the compound of Formula (I) inhibits one or more proteins in the Wnt pathway.

In some embodiments, the compound of Formula (I) inhibits signaling induced by one or more Wnt proteins.

In some embodiments, the Wnt proteins are chosen from: WNT1, WNT2, WNT2B, WNT3, WNT3A, WNT4, WNT5A, WNT5B, WNT6, WNT7A, WNT7B, WNT8A, WNT8B, WNT9A, WNT9B, WNT10A, WNT10B, WNT11, and WNT16.

In some embodiments, the method inhibits one or more proteins in the Wnt pathway, the method comprises contacting a cell with an effective amount of a compound of Formula (I).

In some embodiments, the cell is a human cell.

In some embodiments, the human cell is a cancerous cell.

In some embodiments, the cancerous cell is a colon cancer cell.

In some embodiments, the contacting is in vitro.

In some embodiments, the compound of Formula (I) inhibits a kinase activity.

In some embodiments, the method treats a disease or disorder mediated by the Wnt pathway in a patient, the method comprises administering to the patient a therapeutically effective amount of a compound (or compounds) of Formula (I), or a pharmaceutically acceptable salt thereof.

In some embodiments, the compound of Formula (I) inhibits one or more Wnt proteins.

In some embodiments, the method treats a disease or disorder mediated by kinase activity in a patient, the method comprises administering to the patient a therapeutically effective amount of a compound (or compounds) of Formula (I), or a pharmaceutically acceptable salt thereof.

In some embodiments, the disease or disorder comprises tumor growth, cell proliferation, or angiogenesis.

In some embodiments, the method inhibits the activity of a protein kinase receptor, the method comprises contacting the receptor with an effective amount of a compound (or compounds) of Formula (I), or a pharmaceutically acceptable salt thereof.

In some embodiments, the method treats a disease or disorder associated with aberrant cellular proliferation in a patient; the method comprises administering to the patient a therapeutically effective amount of a compound (or compounds) of Formula (I), or a pharmaceutically acceptable salt thereof.

In some embodiments, the method prevents or reduces angiogenesis in a patient; the method comprises administering to the patient a therapeutically effective amount of a compound (or compounds) of Formula (I), or a pharmaceutically acceptable salt thereof.

In some embodiments, the method prevents or reduces abnormal cellular proliferation in a patient; the method comprises administering to the patient a therapeutically effective amount of a compound (or compounds) of Formula (I), or a pharmaceutically acceptable salt thereof.

In some embodiments, the method treats a disease or disorder associated with aberrant cellular proliferation in a patient, the method comprises administering to the patient a pharmaceutical composition comprising one or more of the compounds of claim 1 in combination with a pharmaceutically acceptable carrier and one or more other agents.

Moreover, the compounds and compositions, for example, as inhibitors of the cyclin-dependent kinases (CDKs), can modulate the level of cellular RNA and DNA synthesis and therefore are expected to be useful in the treatment of viral infections such as HIV, human papilloma virus, herpes virus, Epstein-Barr virus, adenovirus, Sindbis virus, pox virus and the like.

Compounds and compositions described herein can inhibit the kinase activity of, for example, CDK/cyclin complexes, such as those active in the G₀. or G.₁ stage of the cell cycle, e.g., CDK2, CDK4, and/or CDK6 complexes.

Evaluation of Biological Activity

The biological activity of the compounds described herein can be tested using any suitable assay known to those of skill in the art, see, e.g., WO 2001/053268 and WO 2005/009997. For example, the activity of a compound may be tested using one or more of the test methods outlined below.

In one example, tumor cells may be screened for Wnt independent growth. In such a method, tumor cells of interest are contacted with a compound (i.e. inhibitor) of interest, and the proliferation of the cells, e.g. by uptake of tritiated thymidine, is monitored. In some embodiments, tumor cells may be isolated from a candidate patient who has been screened for the presence of a cancer that is associated with a mutation in the Wnt signaling pathway. Candidate cancers include, without limitation, those listed above.

In another example, one may utilize in vitro assays for Wnt biological activity, e.g. stabilization of β-catenin and promoting growth of stem cells. Assays for biological activity of Wnt include stabilization of β-catenin, which can be measured, for example, by serial dilutions of a candidate inhibitor composition. An exemplary assay for Wnt biological activity contacts a candidate inhibitor with cells containing constitutively active Wnt/β-catenin signaling. The cells are cultured for a period of time sufficient to stabilize β-catenin, usually at least about 1 hour, and lysed. The cell lysate is resolved by SDS PAGE, then transferred to nitrocellulose and probed with antibodies specific for β-catenin.

In a further example, the activity of a candidate compound can be measured in a Xenopus secondary axis bioassay (Leyns, L. et al. Cell (1997), 88(6), 747-756).

To further illustrate this invention, the following examples are included. The examples should not, of course, be construed as specifically limiting the invention. Variations of these examples within the scope of the claims are within the purview of one skilled in the art and are considered to fall within the scope of the invention as described, and claimed herein. The reader will recognize that the skilled artisan, armed with the present disclosure, and skill in the art is able to prepare and use the invention without exhaustive examples.

EXAMPLES

Compound Preparation

The starting materials used in preparing the compounds of the invention are known, made by known methods, or are commercially available. It will be apparent to the skilled artisan that methods for preparing precursors and functionality related to the compounds claimed herein are generally described in the literature. The skilled artisan given the literature and this disclosure is well equipped to prepare any of the compounds.

It is recognized that the skilled artisan in the art of organic chemistry can readily carry out manipulations without further direction, that is, it is well within the scope and practice of the skilled artisan to carry out these manipulations. These include reduction of carbonyl compounds to their corresponding alcohols, oxidations, acylations, aromatic substitutions, both electrophilic and nucleophilic, etherifications, esterification and saponification and the like. These manipulations are discussed in standard texts such as March's Advanced Organic Chemistry: Reactions, Mechanisms, and Structure 7^th Ed., John Wiley & Sons (2013), Carey and Sundberg, Advanced Organic Chemistry 5^th Ed., Springer (2007), Comprehensive Organic Transformations: A Guide to Functional Group Transformations, 2^nd Ed., John Wiley & Sons (1999) (incorporated herein by reference in its entirety) and the like.

The skilled artisan will readily appreciate that certain reactions are best carried out when other functionality is masked or protected in the molecule, thus avoiding any undesirable side reactions and/or increasing the yield of the reaction. Often the skilled artisan utilizes protecting groups to accomplish such increased yields or to avoid the undesired reactions. These reactions are found in the literature and are also well within the scope of the skilled artisan. Examples of many of these manipulations can be found for example in T. Greene and P. Wuts Protective Groups in Organic Synthesis, 4th Ed., John Wiley & Sons (2007), incorporated herein by reference in its entirety.

Trademarks used herein are examples only and reflect illustrative materials used at the time of the invention. The skilled artisan will recognize that variations in lot, manufacturing processes, and the like, are expected. Hence the examples, and the trademarks used in them are non-limiting, and they are not intended to be limiting, but are merely an illustration of how a skilled artisan may choose to perform one or more of the embodiments of the invention.

(¹H) nuclear magnetic resonance spectra (NMR) were measured in the indicated solvents on a Bruker NMR spectrometer (Avance™ DRX300, 300 MHz for ¹H or Avance™ DRX500, 500 MHz for ¹H) or Varian NMR spectrometer (Mercury 400BB, 400 MHz for ¹H). Peak positions are expressed in parts per million (ppm) downfield from tetramethylsilane. The peak multiplicities are denoted as follows, s, singlet; d, doublet; t, triplet; q, quartet; ABq, AB quartet; quin, quintet; sex, sextet; sep, septet; non, nonet; dd, doublet of doublets; ddd, doublet of doublets of doublets; d/ABq, doublet of AB quartet; dt, doublet of triplets; td, triplet of doublets; dq, doublet of quartets; m, multiplet.

The following abbreviations have the indicated meanings:

• • BH₃-Me₂S=borane dimethyl sulfide complex
  • (Boc)₂O=di-tert-butyl dicarbonate
  • brine=saturated aqueous sodium chloride
  • CDCl₃=deuterated chloroform
  • CD₃OD=deuterated methanol
  • DCAD=di-(4-chlorobenzyl)azodicarboxylate
  • DCE=dichloroethane
  • DCM=dichloromethane
  • DEAD=diethyl azodicarboxylate
  • DHP=dihydropyran
  • DMAP=4-dimethylaminopyridine
  • DMF=N,N-dimethylformamide
  • DMSO-d₆=deuterated dimethylsulfoxide
  • ESIMS=electron spray mass spectrometry
  • EtOAc=ethyl acetate
  • EtOH=ethanol
  • HCl=hydrochloric acid
  • HOAc=acetic acid
  • K₂CO₃=potassium carbonate
  • KOAc=potassium acetate
  • LDA=lithium diisopropylamide
  • LC/MS=liquid chromatography-mass spectrometry
  • MeOH=methanol
  • MgSO₄=magnesium sulfate
  • MsCl=methanesulfonyl chloride or mesyl chloride
  • MW=microwave
  • NaBH₄=sodium borohydride
  • NaBH(OAc)₃=sodium triacetoxyborohydride
  • NaCNBH₃=sodium cyanoborohydride
  • NaHCO₃=sodium bicarbonate
  • NaOH=sodium hydroxide
  • Na₂S₂O₅=sodium metabisulfite or sodium pyrosulfite
  • NH₄OH=ammonium hydroxide
  • NMR=nuclear magnetic resonance
  • ON=overnight
  • Pd/C=palladium(0) on carbon
  • Pd(dppf)Cl₂=1,1′-bis(diphenylphosphino)ferrocene]palladium(II) chloride
  • Pd(PPh₃)₄=tetrakis(triphenylphosphine)palladium(0)
  • Pd(PPh₃)₂Cl₂=bis(triphenylphosphine)palladium(II) dichloride
  • PE=petroleum ether
  • Pin₂B₂=bis(pinacolato)diboron
  • PPh₃=triphenylphosphine
  • PPTS=pyridinium p-toluenesulfonate
  • r.t.=room temperature
  • SEM-Cl=2-(trimethylsilyl)ethoxymethyl chloride
  • TEA=triethylamine
  • TFA=trifluoroacetic acid
  • THF=tetrahydrofuran
  • THP=tetrahydropyran
  • TLC=thin layer chromatography
  • p-TsOH=p-toluenesulfonic acid

The following example schemes are provided for the guidance of the reader, and collectively represent an example method for making the compounds provided herein. Furthermore, other methods for preparing compounds of the invention will be readily apparent to the person of ordinary skill in the art in light of the following reaction schemes and examples. The skilled artisan is thoroughly equipped to prepare these compounds by those methods given the literature and this disclosure. The compound numberings used in the synthetic schemes depicted below are meant for those specific schemes only, and should not be construed as or confused with same numberings in other sections of the application. Unless otherwise indicated, all variables are as defined above.

General Procedure

Compounds of Formula (I) of the present invention can be prepared as depicted in Scheme 1.

Scheme 1 describes the method for preparation of 1H-pyrazolo[3,4-b]pyridine derivatives (VIII) by reacting 5-bromo-1-(tetrahydro-2H-pyran-2-yl)-1H-pyrazolo[3,4-b]pyridine-3-carbaldehyde (II) with bis(pinacolato)diboron to form the borate ester (III). Suzuki coupling with various halides (IV) yields 1H-pyrazolo[3,4-b]pyridine derivatives (V). Aldehyde (V) is reacted with various 1,2-diamines (VI) to produce (VII). Final deprotection of the pyrazole nitrogen yields the desired 1H-pyrazolo[3,4-b]pyridine derivatives (VIII).

Illustrative Compound Examples

Synthesis of intermediate 5-bromo-1-(tetrahydro-2H-pyran-2-yl)-1H-pyrazolo[3,4-b]pyridine-3-carbaldehyde (XIX) is depicted below in Scheme 2.

Step 1

A solution of 2-chloropyridine (IX) (9.39 mL, 0.1 mol) in anhydrous THF (50 mL) was added slowly to a solution of LDA (2.0 M solution in THF/hexane/ethylbenzene, 50 mL, 0.1 mol) in THF (200 mL) stirred at −78° C. under nitrogen. The stirring was continued at −78° C. for an additional 3 h before adding acetaldehyde (6.17 mL, 0.110 mol). The solution was stirred at −78° C. for another 2 h before allowing the temperature to rise to −40° C. A solution of water (4 mL) in THF (40 mL) was added slowly to the solution. When the temperature reached −10° C., additional water (200 mL) was added to the solution. The solution was extracted with ethyl ether (3×100 mL). The combined organic phase was dried over MgSO₄, filtered and evaporated under reduced pressure to get a brown viscous residue. The crude product was purified on a flash silica gel column (1:1 DCM:hexane→100% DCM) to produce 1-(2-chloropyridin-3-yl)ethanol (X) as a brown viscous oil (6 g, 38.1 mmol, 38% yield). ¹H NMR (CDCl₃) δ ppm 1.52 (d, J=6.41 Hz, 3H), 2.51 (brs, 1H), 5.24 (m, 1H), 7.28 (m, 1H), 7.97 (dd, J=7.72 Hz, J=1.70 Hz, 1H), 8.27 (dd, J=7.72 Hz, J=1.79 Hz, 1H).

Step 2

To a solution of 1-(2-chloropyridin-3-yl)ethanol (X) in dry acetone at −30° C. under nitrogen was added in portions chromium (VI) oxide (1.80 g, 18 mmol). The solution was further stirred 15 min at −30° C. and allowed to warm to room temperature. The solution was stirred for 3 h at room temperature before adding isopropanol (10 mL). The solution was made alkaline by slowly adding a saturated aqueous NaHCO₃ solution. The solution was filtered through a bed of Celite. The solids were washed by DCM. The organic phase of the filtrate was separated and the aqueous phase extracted with DCM (2×50 mL). The combined organic layers were dried over MgSO₄, filtered and concentrated under reduced pressure to yield 1-(2-chloropyridin-3-yl)ethanone (XI) as a brown liquid (0.72 g, 4.63 mmol, 77% yield). ¹H NMR (CDCl₃) δ ppm 2.71 (s, 3H), 7.35 (dd, J=7.63 Hz, J=4.80 Hz, 1H), 7.91 (dd, J=7.54 Hz, J=1.88 Hz, 1H), 8.55 (dd, J=4.71 Hz, J=1.88 Hz, 1H).

Step 3

To a solution of 1-(2-chloropyridin-3-yl)ethanone (XI) (0.311 g, 2 mmol) in n-butanol (10 mL) was added hydrazine hydrate (1.45 mL, 30 mmol). The reaction was refluxed overnight. The solution was cooled and the solvent was evaporated under vacuum. The residue was dissolved in DCM and washed successively by water and brine. The organic layers were dried over MgSO₄, filtered and concentrated under reduced pressure to give 3-methyl-1H-pyrazolo[3,4-b]pyridine (XII) as a white solid (192 mg, 1.44 mmol, 72% yield). ¹H NMR (CDCl₃) δ ppm 2.64 (s, 3H), 7.14 (dd, J=8.01 Hz, J=4.62 Hz, 1H), 8.14 (dd, J=7.54 Hz, J=1.88 Hz, 1H), 8.59 (dd, J=4.52 Hz, J=1.32 Hz, 1H), 11.68 (brs, 1H).

Step 4

To a solution of NaOH (0.88 g, 22 mmol) in water (20 mL) was added 3-methyl-1H-pyrazolo[3,4-b]pyridine (XII) (0.4 g, 3 mmol). The suspension was heated at 80° C. until a clear solution was obtained. A solution of KMnO₄ (1.73 g, 11 mmol) in water (180 mL) was added slowly over 2 h while heating the solution at 80° C. The solution was heated at 90° C. for an additional 2 h until the complete disappearance of starting material was observed by TLC. The solution was cooled to 70° C. and filtered through a pad of Celite. The solids were washed by boiling water. The combined filtrate was cooled to 0° C., acidified with conc. H₂SO₄ to pH=2 and extracted with n-butanol (2×10 mL). The n-butanol layer was concentrated under reduced pressure to get a white residue which was dissolved in DCM by adding minimum amount of MeOH and then filtered. The filtrate was concentrated to give 1H-pyrazolo[3,4-b]pyridine-3-carboxylic acid (XIII) as a white solid (390 mg, 2.39 mmol, 81% yield). ¹H NMR (CDCl₃) δ ppm 7.37 (dd, J=8.10 Hz, J=4.52 Hz, 1H), 8.47 (dd, J=7.54 Hz, J=1.88 Hz, 1H), 8.62 (dd, J=4.52 Hz, J=1.32 Hz, 1H), 14.37 (brs, 1H).

Step 5

To a solution of 1H-pyrazole[3,4-b]pyridine-3-carboxylic acid (XIII) (0.39 g, 2.4 mmol) in dry MeOH (10 mL) was added concentrated H₂SO₄ (4 drops) and refluxed for 6 h under nitrogen. The solution was cooled and the solvent was evaporated under vacuum. The residue was partitioned between DCM and saturated aqueous NaHCO₃ solution. The organic layer was separated, dried over MgSO₄, filtered and concentrated under reduced pressure. The crude product was purified on a flash silica gel column (100% DCM→3:97 MeOH:DCM) to produce methyl 1H-pyrazolo[3,4-b]pyridine-3-carboxylate (XIV) as a white solid (382 mg, 2.16 mmol, 90% yield). ¹H NMR (CDCl₃) δ ppm 4.08 (s, 3H), 7.38 (m, 1H), 8.63 (dd, J=8.10 Hz, J=1.51 Hz, 1H), 8.72 (dd, J=4.62 Hz, J=1.41 Hz, 1H); ESIMS found for C₈H₇N₃O₂ m/z 178.2 (M+H).

Step 6

A mixture of methyl 1H-pyrazolo[3,4-b]pyridine-3-carboxylate (XIV) (0.177 g, 1 mmol), sodium acetate (0.492 g, 6 mmol) and bromine (0.308 mL, 6 mmol) in glacial acetic acid (5 mL) was heated overnight at 120° C. in a sealed tube. The solution was cooled and poured into water. The solids formed were filtered, washed with water and dried at room temperature under vacuum. The crude product was purified on a flash silica gel column (100% DCM→2:98 MeOH:DCM) to produce methyl 5-bromo-1H-pyrazolo[3,4-b]pyridine-3-carboxylate (XV) as a white solid (78 mg, 0.31 mmol, 30% yield). ¹H NMR (CDCl₃) δ ppm 3.95 (s, 3H), 8.62 (d, J=3.01 Hz, 1H), 8.73 (d, J=3.01 Hz, 1H); ESIMS found for C₈H₆BrN₃O₂ m/z 256.3 (M+H).

Step 7

A suspension of methyl 5-bromo-1H-pyrazolo[3,4-b]pyridine-3-carboxylate (XV) (70 mg, 0.27 mmol) in aqueous 1N NaOH solution (20 mL) was heated at 90° C. for 3 h until the solution became clear. The solution was then cooled to 0° C. and acidified with a 10% HCl solution. The solids formed were filtered, washed with cold water and dried at room temperature under vacuum to give 5-bromo-1H-pyrazolo[3,4-b]pyridine-3-carboxylic acid (XVI) as a white solid (60 mg, 0.25 mmol, 92% yield). ¹H NMR (CDCl₃) δ ppm 8.58 (d, J=3.01 Hz, 1H), 8.66 (d, J=3.01 Hz, 1H); ESIMS found for C₇H₄BrN₃O₂ m/z 242.1 (M+H).

Step 8

To a solution of 5-bromo-1H-pyrazole[3,4-b]pyridine-3-carboxylic acid (XVI) (0.242 g, 1 mmol) in dry DMF (5 mL) was added CDI (0.178 g, 1.1 mmol) and heated for 3 h at 65° C. under nitrogen. The solution was cooled to room temperature and N,O-dimethyl hydroxylamine hydrochloride (0.107 g, 1.1 mmol) was added to the solution. The solution was again heated for 3 h at 65° C. under nitrogen. The solution was cooled and the solvent was evaporated under reduced pressure. The residue was dissolved in DCM, washed successively with a 10% HCl solution, a saturated aqueous NaHCO₃ solution and brine. The organic phase was dried over MgSO₄, filtered and concentrated under reduced pressure to produce 5-bromo-N-methoxy-N-methyl-1H-pyrazolo[3,4-b]pyridine-3-carboxamide (XVII) as a white solid (260 mg, 0.91 mmol, 92% yield). ¹H NMR (CDCl₃) δ ppm 3.55 (s, 3H), 3.78 (s, 3H), 8.59 (d, J=3.01 Hz, 1H), 8.67 (d, J=3.01 Hz, 1H); ESIMS found for C₉H₉BrN₄O₂ m/z 285.4 (M+H).

Step 9

To a solution of 5-bromo-N-methoxy-N-methyl-1H-pyrazolo[3,4-b]pyridine-3-carboxamide (XVII) (0.250 g, 0.88 mmol) in dry DCM (10 mL) was added 3,4-dihydro-2H-pyran (0.179 mL, 1.98 mmol) and PPTS (22 mg, 0.08 mmol) and refluxed 5 h under nitrogen. Another equivalent of 3,4-dihydro-2H-pyran (0.179 mL, 1.98 mmol) and PPTS (22 mg, 0.08 mmol) was added and the solution was further heated at refluxed overnight under nitrogen. The solution was cooled, diluted with DCM, washed subsequently with a saturated aqueous NaHCO₃ solution and brine. The organic layer was dried over MgSO₄, filtered and concentrated under reduced pressure to give 5-bromo-N-methoxy-N-methyl-1-(tetrahydro-2H-pyran-2-yl)-1H-pyrazolo[3,4-b]pyridine-3-carboxamide (XVIII) as a viscous liquid (302 mg, 0.82 mmol, 93% yield). ¹H NMR (CDCl₃) δ ppm 1.51-1.62 (m, 2H), 1.91-2.13 (m, 2H), 2.33-2.44 (m, 2H), 3.40 (s, 3H), 3.66 (m, 1H), 3.75 (s, 3H), 3.87-3.98 (m, 1H), 6.07 (dd, J=10.07 Hz, J=2.52 Hz, 1H), 8.57 (d, J=3.01 Hz, 1H), 8.73 (d, J=3.01 Hz, 1H); ESIMS found for C₁₄H₁₇BrN₄O₃ m/z 369.4 (M+H).

Step 10

To a solution of 5-bromo-N-methoxy-N-methyl-1-(tetrahydro-2H-pyran-2-yl)-1H-pyrazolo[3,4-b]pyridine-3-carboxamide (XVIII) (0.290 g, 0.78) in dry THF (5 mL) stirred at 0° C. under nitrogen was added lithium aluminum hydride (36 mg, 0.94 mmol). The solution was further stirred at 0° C. for 30 min. The reaction was quenched with a 0.4 N NaHSO₄ solution (10 mL). The solution was extracted with DCM (3×15 mL). The combined organic layer was washed subsequently with water and brine. The organic layer was dried over MgSO₄, filtered and concentrated under reduced pressure to produce 1-(tetrahydro-2H-pyran-2-yl)-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-1H-pyrazolo[3,4-b]pyridine-3-carbaldehyde (XIX) as a viscous liquid (218 mg, 0.70 mmol, 91% yield). ¹H NMR (CDCl₃) δ ppm 1.52-1.74 (m, 2H), 1.95-2.18 (m, 2H), 2.37-2.49 (m, 2H) 3.87-3.98 (m, 1H), 3.99 (m, 1H), 6.18 (dd, J=10.20 Hz, J=2.39 Hz, 1H), 8.73 (d, J=3.01 Hz, 1H), 8.85 (d, J=3.01 Hz, 1H), 10.16 (s, 1H); ESIMS found for C₁₂H₁₂BrN₃O₂ m/z 310.4 (M+H).

Preparation of intermediate N-(5-bromopyridin-3-yl)pivalamide (XXII) is depicted below in Scheme 3.

Step 1

To a solution of 3-amino-5-bromo pyridine (XX) (1.0 g, 5.78 mmol) in dry pyridine (10 mL) was added pivaloyl chloride (XXI) (769 mg, 6.38 mmol). The reaction mixture was stirred at room temperature for 3 h. The reaction was poured into an ice water/saturated aqueous NaHCO₃ mixture and stirred for 30 min. The precipitate was filtered, washed with cold water and dried at room temperature to yield N-(5-bromopyridin-3-yl)pivalamide (XXII) as an off-white solid (1.082 g, 4.22 mmol, 73.1% yield). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 1.23 (s, 9H), 8.37 (d, J=2 Hz, 1H), 8.39 (t, J=2 Hz, 1H), 8.80 (d, J=2 Hz, 1H), 9.58 (brs, 1H); ESIMS found C₁₀H₁₃BrN₂O m/z 258.9 (Br⁸¹M+H).

The following intermediates were prepared in accordance with the procedure described in the above Scheme 3.

N-(5-Bromopyridin-3-yl)isobutyramide (XXIII): Off-white solid, (71% yield). ¹H NMR (CDCl₃) δ ppm 8.55-8.35 (m, 3H), 7.32 (s, 1H), 2.59-2.48 (m, 1H), 1.28-1.27 (d, 6H); ESIMS found C₉H₁₁BrN₂O m/z 242.9 (Br⁷⁹M+H).

N-(5-Bromopyridin-3-yl)propionamide (XXIV): Off white solid (92% yield). ¹H NMR (DMSO-d₆) δ ppm 1.09 (t, J=7.54 Hz, 3H), 2.36 (q, J=7.54 Hz, 2H), 8.36 (m, 2H), 8.65 (d, J=2.07 Hz, 1H), 10.26 (s, 1H); ESIMS found C₈H₉BrN₂O m/z 231.1 (Br⁸¹M+H).

N-(5-Bromopyridin-3-yl)butyramide (XXV): Yellow solid (2.1 g, 8.64 mmol, 88.8% yield). ¹H NMR (CD₃OD, 400 MHz) δ ppm 1.02 (t, J=7.2 Hz, 3H), 1.74 (sxt, J=7.2 Hz, 2H), 2.40 (t, J=7.2 Hz, 2H), 8.35 (d, J=2 Hz, 1H), 8.46 (t, J=2 Hz, 1H), 8.63 (d, J=2 Hz, 1H); ESIMS found C₉H₁₁BrN₂O m/z 243.1 (Br⁷⁹M+H).

N-(5-Bromopyridin-3-yl)pentanamide (XXVI): Yellow solid (2.0 g, 7.78 mmol, 85.3% yield). ¹H NMR (CD₃OD, 400 MHz) δ ppm 0.98 (t, J=7.4 Hz, 3H), 1.43 (sxt, J=7.4 Hz, 2H), 1.70 (quin, J=7.4 Hz, 2H), 2.43 (t, J=7.6 Hz, 2H), 8.35 (s, 1H), 8.45 (d, J=2 Hz, 1H), 8.64 (d, J=2 Hz, 1H); ESIMS found C₁₀H₁₃BrN₂O m/z 256.9 (Br⁷⁹M+H).

N-(5-Bromopyridin-3-yl)-3-methylbutanamide (XXVII): Off white solid, (67% yield), ¹H NMR (CDCl₃, 500 MHz) δ ppm 8.55-8.42 (m, 3H), 7.62 (s, 1H), 2.31-2.18 (m, 3H), 1.02-1.01 (d, J=6 Hz, 6H); ESIMS found C₁₀H₁₃BrN₂O m/z 258.9 (Br⁸¹M+H).

N-(5-Bromopyridin-3-yl)-3,3-dimethylbutanamide (XXVIII): Yellow solid (1.7 g, 6.27 mmol, 78.6% yield). ¹H NMR (CD₃OD, 400 MHz) δ ppm 1.10 (s, 9H), 2.29 (s, 2H), 8.36 (d, J=1.6 Hz, 1H), 8.46 (d, J=2.0 Hz, 1H), 8.64 (d, J=2.0 Hz, 1H); ESIMS found C₁₁H₁₅BrN₂O m/z 273.1 ((Br⁸¹M+H).

N-(5-Bromopyridin-3-yl)-2-phenylacetamide (XXIX): White solid (2.5 g, 8.59 mmol, 77.9% yield). ¹H NMR (CDCl₃, 400 MHz) δ ppm 3.76 (s, 2H), 7.26-7.45 (m, 5H), 7.57 (brs, 1H), 8.33 (s, 1H), 8.37 (s, 2H); ESIMS found C₁₃H₁₁BrN₂O m/z 292.8 (Br⁸¹M+H).

N-(5-Bromopyridin-3-yl)benzamide (XXX): White solid (2.7 g, 9.74 mmol, 60% yield). ¹H NMR (CDCl₃, 400 MHz) δ ppm 7.40-7.52 (m, 2H), 7.52-7.62 (m, 1H), 7.86 (d, J=7.2 Hz, 2H), 8.39 (d, J=1.6 Hz, 1H), 8.46 (s, 1H), 8.55 (d, J=1.6 Hz, 1H), 8.57 (d, J=2.0 Hz, 1H); ESIMS found C₁₂H₉BrN₂O m/z 278.8 (Br⁸¹M+H).

N-(5-Bromopyridin-3-yl)cyclopropanecarboxamide (XXXI): Off-white solid, (83% yield), ¹H NMR (CDCl₃, 500 MHz) δ ppm 8.46-8.39 (m, 3H), 7.54 (bs, 1H), 1.56-1.50 (m, 1H), 1.13-1.07 (m, 2H), 0.96-0.90 (m, 2H); ESIMS found for C₉H₉BrN₂O m/z 240.9 (Br⁷⁹M+H).

N-(5-Bromopyridin-3-yl)cyclobutanecarboxamide (XXXII): Yellow solid (2.1 g, 6.27 mmol, 86.6% yield). ¹H NMR (CD₃OD, 400 MHz) δ ppm 1.80-1.99 (m, 1H), 1.99-2.15 (m, 1H), 2.16-2.30 (m, 2H), 2.30-2.45 (m, 2H), 3.25-3.35 (m, 1H), 8.34 (d, J=2.0 Hz, 1H), 8.47 (s, 1H), 8.64 (d, J=2.0 Hz, 1H); ESIMS found C₁₀H₁₁BrN₂O m/z 257.1 (Br⁸¹M+H).

N-(5-Bromopyridin-3-yl)cyclopentanecarboxamide (XXXIII): Yellow solid (1.9 g, 7.06 mmol, 80.2% yield). ¹H NMR (CD₃OD, 400 MHz) δ ppm 1.57-1.74 (m, 2H), 1.74-1.91 (m, 4H), 1.91-2.07 (m, 2H), 2.77-2.92 (m, 1H), 8.34 (d, J=1.6 Hz, 1H), 8.45 (s, 1H), 8.65 (d, J=2.0 Hz, 1H); ESIMS found C₁₁H₁₃BrN₂O m/z 271.1 (Br⁸¹M+H).

N-(5-bromopyridin-3-yl)cyclohexanecarboxamide (XXXIV): Yellow solid (2.0 g, 7.06 mmol, 84.3% yield). ¹H NMR (CD₃OD, 400 MHz) δ ppm 1.19-1.46 (m, 3H), 1.46-1.63 (m, 2H), 1.74 (d, J=11.6 Hz, 1H), 1.88 (t, J=14.0 Hz, 4H), 2.40 (tt, J=11.6 Hz, J=3.6 Hz, 1H), 8.34 (d, J=2.0 Hz, 1H), 8.44 (t, J=2.0 Hz, 1H), 8.64 (d, J=2.0 Hz, 1H); ESIMS found C₁₂H₁₅BrN₂O m/z 285.1 (Br⁸¹M+H).

N-(5-bromopyridin-3-yl)-2-cyclohexylacetamide (XXXV): Yellow solid (261 mg, 0.878 mmol, 84.4% yield). ESIMS found C₁₃H₁₇BrN₂O m/z 297.1 (Br⁸¹M+H).

Preparation of intermediate 5-bromo-N,N-dimethylpyridin-3-amine (XXXVII) is depicted below in Scheme 4.

Step 1

To a solution of 3,5-dibromopyridine (XXXVI) (2.37 g, 10.0 mmol) in dry DMF (20.0 mL) was added K₂CO₃ (4.5 g, 33 mmol) and dimethylamino hydrochloride (1.79 g, 22 mmol). The mixture was heated overnight at 200° C. in a sealed tube. The solution was cooled to room temperature and excess DMF was removed under vacuum. The residue was partitioned between EtOAc and water. The organic phase was separated. The aqueous phase was washed with EtOAc and the combined organic phases were dried over MgSO₄, and concentrated to afford 5-bromo-N,N-dimethylpyridin-3-amine (XXXVII) as an off-white solid (1.78 g, 8.85 mmol, 88% yield). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 2.94 (s, 6H), 7.25 (t, J=2 Hz, 1H), 7.91 (d, J=2 Hz, 1H), 8.07 (d, J=2 Hz, 1H); ESIMS found C₇H₉BrN₂ m/z 201.1 (M+H).

Preparation of intermediate 5-bromo-N-isopropylpyridin-3-amine (XXXVIII) is depicted below in Scheme 5.

Step 1

To a solution of 5-bromopyridin-3-amine (XX) (535 mg, 3.09 mmol) in MeOH (62 mL) was added acetone (296 μL, 4.02 mL). The pH was adjusted to 4 using HOAc and stirred for 30 min. NaCNBH₃ (272 mg, 4.33 mmol) was added and stirred at room temperature overnight. The MeOH was removed under vacuum and the residue was partitioned between EtOAc and saturated aqueous NaHCO₃. The organic layer was dried over MgSO₄ and evaporated under vacuum. The crude product was purified on a silica gel column (100% hexane→90:10 hexane:EtOAc) to produce 5-bromo-N-isopropylpyridin-3-amine (XXXVIII) as an oil which slowly solidified into an off-white solid (309 mg, 1.44 mmol, 47% yield). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 1.12 (d, J=6.3 Hz, 6H), 3.55-3.59 (m, 1H), 6.03 (d, J=7.9 Hz, 1H), 7.05-7.06 (m, 1H), 7.75 (d, J=2 Hz, 1H), 7.90 (d, J=2 Hz, 1H); ESIMS found C₈H₁₁BrN₂ m/z 215.1 (M+H).

Preparation of intermediate 1-(5-bromopyridin-3-yl)-N,N-dimethylmethanamine (XL) is depicted below in Scheme 6.

Step 1

Preparation of 1-(5-bromopyridin-3-yl)-N,N-dimethylmethanamine (XL) was performed following the procedure listed in Scheme 5, Step 1. Brown oil (1.20 g, 5.59 mmol, 45% yield). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 2.15 (s, 6H), 3.43 (s, 2H), 7.94 (s, 1H), 8.47 (d, J=1.1 Hz, 1H), 8.59 (d, J=2.2 Hz, 1H); ESIMS found C₈H₁₁BrN₂ m/z 215 (M^Br79+H) and 217 (M^Br81+H).

Preparation of intermediate 3-bromo-5-((3,3-difluoropyrrolidin-1-yl)methyl)pyridine (XLI) is depicted below in Scheme 7.

Step 1

To a mixture of 5-bromopyridine-3-carbaldehyde (XXXIX) (6.00 g, 32.26 mmol, 1.0 eq), 3,3-difluoropyrrolidine (5.56 g, 38.71 mmol, 1.20 eq) and TEA (5.39 mL, 38.71 mmol, 1.2 Eq) in DCE (200 mL) was stirred at room temperature for 30 min, then added sodium triacetoxyborohydride (10.25 g, 48.38 mmol, 1.50 eq) in one portion at room temperature under N₂. The mixture was stirred at room temperature for 6 hours. TLC showed the reaction was complete. The reaction was quenched with 1N NaOH (100 mL), extracted with DCE (100 mL×2). The combined organic layers were washed with brine (100 mL), dried and concentrated. The residue was purified by silica gel chromatography (column height: 50 mm, diameter: 50 mm, 300-400 mesh silica gel, DCM/MeOH=30/1→20/1) to give 3-bromo-5-((3,3-difluoropyrrolidin-1-yl)methyl) pyridine (XLI): Yellow oil (8.00 g, 28.9 mmol, 89.5% yield). ¹H NMR (CDCl₃, 400 MHz) δ ppm 2.30 (spt, J=7.2 Hz. 2H), 2.75 (t, J=6.8 Hz, 2H), 2.91 (t, J=13.2 Hz, 2H), 7.85 (s, 1H), 8.45 (s, 1H), 8.59 (d, J=2 Hz, 1H); ESIMS found for C₁₀H₁₁BrF₂N₂ m/z 277.0 (M+H).

The following intermediates were prepared in accordance with the procedure described in the above Scheme 6 or Scheme 7.

3-Bromo-5-(pyrrolidin-1-ylmethyl)pyridine (XLII): Golden liquid (1.35 g, 97% yield). ¹H NMR (DMSO-d₆) 1.68-1.71 (m, 4H), 2.42-2.44 (m, 4H), 3.60 (s, 2H), 7.96 (s, 1H), 8.48 (d, J=2 Hz, 1H), 8.58 (d, J=3 Hz, 1H); ESIMS found for C₁₀H₁₃BrN₂ m/z 242.2 (M+H).

3-Bromo-5-(piperidin-1-ylmethyl)pyridine (XLIII): Brown liquid (13.1 g, 94% yield). ¹H NMR (DMSO-d₆) 1.36-1.39 (m, 2H), 1.46-1.51 (m, 4H), 2.31-2.32 (m, 4H), 3.46 (s, 2H), 7.94 (s, 1H), 8.47 (d, J=2 Hz, 1H), 8.58 (d, J=3 Hz, 1H); ESIMS found for C₁₁H₁₅BrN₂ m/z 257.0 (M+H).

N-((5-Bromopyridin-3-yl)methyl)ethanamine (XLIV): Golden liquid (1.29 g, 6.00 mmol, 60% yield). ¹H NMR (CDCl₃, 400 MHz) δ ppm 1.14 (t, J=7.2 Hz, 3H), 2.67 (q, J=7.2 Hz, 2H), 3.79 (s, 2H), 7.85 (t, J=2 Hz, 1H), 8.46 (d, J=1.6 Hz, 1H), 8.56 (d, J=2.4 Hz, 1H); ESIMS found for C₈H₁₁BrN₂ m/z 215.1 (M+H).

N-Benzyl-1-(5-bromopyridin-3-yl)methanamine (XLV): Yellow oil (8.0 g, 28.9 mmol, 89.5% yield). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 3.71 (s, 2H), 3.74 (s, 2H), 7.18-7.28 (m, 1H), 7.28-7.40 (m, 4H), 8.04 (s, 1H), 8.52 (s, 1H), 8.58 (s, 1H); ESIMS found for C₁₃H₁₃BrN₂ m/z 277.1 (M+H).

Preparation of intermediate tert-butyl (5-bromopyridin-3-yl)methyl (cyclopentylmethyl)carbamate (L) is depicted below in Scheme 8.

Step 1

To a solution of 5-bromonicotinaldehyde (XXXIX) (2.0 g, 10.8 mmol, 1 eq) in MeOH (20 mL) was added NaBH₄ (2.4 g, 64.9 mmol, 6 eq) and the reaction mixture was stirred at room temperature for 3 h. The mixture was concentrated in vacuo and the residue was diluted in water (15 mL), the aqueous phase was extracted with DCM (10 mL×3). The combined organic layers were dried over MgSO₄, filtered and concentrated in vacuo to afford (5-bromopyridin-3-yl)methanol (XLVI) (1.8 g, 9.57 mmol, 90.0% yield) as a colorless oil. ¹H NMR (CDCl₃, 500 MHz) δ ppm 4.73 (s, 2H), 7.90 (s, 1H), 8.47 (s, 1H), 8.57 (s, 1H). ESIMS found for C₆H₆BrNO m/z 188.0 (M+H).

Step 2

To a stirred solution of (5-bromopyridin-3-yl)methanol (XLVI) (1.60 g, 8.5 mmol, 1 eq), phthalimide (1.24 g, 8.5 mmol, 1 eq) and PPh₃ (3.33 g, 12.75 mmol, 1.5 eq) in anhydrous THF (15 mL) was added DEAD (2.21 g, 12.75 mmol, 1.5 eq) dropwise at 0° C. under N₂. Then the reaction mixture was stirred at room temperature for 6 h. The mixture was washed with saturated NaHCO₃ solution (15 mL), water (15 mL) and brine (15 mL) subsequently. The organic layers were dried over MgSO₄, concentrated under reduced pressure, the resultant residue was purified by flash chromatography on silica gel (PE:EtOAc=4:1) to give 2-((5-bromopyridin-3-yl)methyl)isoindoline-1,3-dione (XLVII) (2.5 g, 7.88 mmol, 82.3% yield) as a white solid. ESIMS found for C₁₄H₉BrN₂O₂ m/z 317.1 (M+H).

Step 3

A solution of 2-((5-bromopyridin-3-yl)methyl)isoindoline-1,3-dione (XLVII) (1.9 g, 6.0 mmol, 1 eq) and hydrazine hydrate (2.0 g, 40 mmol, 6 eq) in EtOH (20 mL) was heated at 70° C. for 3 h. The mixture was filtered through a Celite® pad and the filtrate was concentrated in vacuo, the crude product was dissolved in 1N HCl solution (15 mL) and concentrated to dryness, then it was washed with acetone (10 mL×3), the precipitate was collected by filtration, dried in vacuo to give (5-bromopyridin-3-yl)methanamine (XLVIII) (1.3 g, 6.95 mmol, 97.7% yield) as a white solid. ¹H NMR (D₂O, 500 MHz) δ ppm 4.34 (s, 2H), 8.56 (s, 1H), 8.75 (d, J=1.2 Hz, 1H), 8.91 (d, J=1.6 Hz, 1H). ESIMS found for C₆H₇BrN₂ m/z 187.0 (M+H).

Step 4

A solution of (5-bromopyridin-3-yl)methanamine (XLVIII) (1.30 g, 5.8 mmol, 1.0 eq), cyclopentanecarbaldehyde (0.57 g, 5.8 mmol, 1.0 eq) and TEA (0.60 g, 5.8 mmol, 1.0 eq) in MeOH (15 mL) was stirred at room temperature for 2 h. Then NaBH₃CN (1.98 g, 34.6 mmol, 6.0 eq) was added and the mixture was stirred at the same temperature for another 3 h. The solvent was removed under reduced pressure and the residue was diluted in water (20 mL) and extracted with DCM (10 mL×3), combined organic layers were dried over MgSO₄ and concentrated in vacuo to give 1-(5-bromopyridin-3-yl)-N-(cyclopentylmethyl)methanamine (XLIX) (1.23 g, 4.57 mmol, 79.3% yield) as a yellow oil. ¹H NMR (CDCl₃, 400 MHz) δ ppm 1.07-1.23 (m, 2H), 1.47-1.67 (m, 4H), 1.70-1.84 (m, 2H), 2.02 (spt, J=7.6 Hz. 1H), 2.53 (d, J=7.2 Hz, 2H), 3.80 (s, 2H), 7.86 (s, 1H), 8.47 (s, 1H), 8.56 (d, J=2.0 Hz, 1H); ESIMS found for C₁₂H₁₇BrN₂ m/z 269.1 (M+H).

Step 5

To a solution of 1-(5-bromopyridin-3-yl)-N-(cyclopentylmethyl) methanamine (XLIX) (1.00 g, 3.7 mmol, 1 eq) and TEA (0.93 g, 9.2 mmol, 2.5 eq) in DCM (20 mL) was added portionwise (Boc)₂O (0.85 g, 4.0 mmol, 1.1 eq) at 0° C., the reaction mixture was stirred at room temperature for 1 h. The mixture was washed with water (10 mL), brine (10 mL), the organic layer was separated, dried over MgSO₄ and concentrated in vacuo to give tert-butyl (5-bromopyridin-3-yl)methyl (cyclopentylmethyl) carbamate (L) (1.25 g, 3.38 mmol, 91.9% yield) as a white solid. ESIMS found for C₁₇H₂₅BrN₂O₂ m/z 369.1 (M+H).

Preparation of intermediate 3-bromo-5-(cyclohexyloxy)pyridine (LIII) is depicted below in Scheme 9.

Step 1

To a solution of 5-bromopyridin-3-ol (LI) (523 mg, 3.01 mmol) in THF (30 mL) cooled to 0° C. were added triphenylphosphine (867 mg, 3.31 mmol) and cyclohexanol (LII) (331 mg, 3.31 mmol) followed by (E)-bis(4-chlorobenzyl) diazene-1,2-dicarboxylate (1.21 g, 3.31 mmol), added portionwise. The reaction mixture was then stirred at 25° C. overnight. The reaction was worked-up with a EtOAc—NaHCO₃ extraction and the solid filtered off. The solvent was removed and the residue was purified by Isco (20% EtOAc-Hexanes) to give 3-bromo-5-(cyclohexyloxy)pyridine (LIII) (209 mg, 0.82 mmol, 27.2% yield) as a yellow oil. ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 1.21-1.31 (m, 1H) 1.34-1.48 (m, 4H) 1.49-1.57 (m, 1H) 1.70 (br dd, J=9.74, 4.25 Hz, 2H) 1.88-1.96 (m, 2H) 2.50 (dt, J=3.70, 1.72 Hz, 5H) 4.46-4.54 (m, 1H) 7.72 (t, J=2.20 Hz, 1H) 8.24 (d, J=1.92 Hz, 1H) 8.27 (d, J=2.47 Hz, 1H).

The following intermediate was prepared in accordance with the procedure described in the above Scheme 9.

tert-Butyl 4-((5-bromopyridin-3-yl)oxy)piperidine-1-carboxylate (LIV): Yellow oil (244 mg, 0.683 mmol, 23.2% yield). ESIMS found for C₁₅H₂₁BrN₂O₃ m/z 358.3 (M+H).

Preparation of intermediate 3-(benzyloxy)-5-bromopyridine (LVI) is depicted below in Scheme 10.

Step 1

To a solution of 5-bromopyridin-3-ol (LI) (174 mg, 1.0 mmol) in DMF (3 mL) was added potassium carbonate (415 mg, 3.0 mmol). The slurry was heated at 90° C. for 1 hour and then cooled to 25° C. The (bromomethyl)benzene (LV) (171 mg, 1.0 mmol) was added and the mixture was stirred at 25° C. overnight. The reaction was worked-up using a saturated sodium bicarbonate and ethyl acetate extraction. The product was purified by ISCO column eluted with 40-100% EtOAc-Hexanes. The 3-(benzyloxy)-5-bromopyridine (LVI) (105 mg, 0.398 mmol, 39.8% yield) was obtained as yellow oil. MS: 266.1. ESIMS found for C₁₂H₁₀BrNO m/z 266.1 (M+H).

The following intermediates were prepared in accordance with the procedure described in the above Scheme 10.

3-Bomo-5-(2-(pyrrolidin-1-yl)ethoxy)pyridine (LVII): Yellow oil ((97 mg, 0.358 mmol, 15.56% yield). ESIMS found for C₁₁H₁₅BrN₂O m/z 272.2 (M+H).

2-((5-bromopyridin-3-yl)oxy)-N,N-dimethylethan-1-amine (LVIII): Yellow oil (97 mg, 0.396 mmol, 28.9% yield). ESIMS found for C₉H₁₃BrN₂O m/z 245.1 (M+H).

1-(2-(3-bromo-5-fluorophenoxy)ethyl)pyrrolidine (LIX): Yellow oil (370 mg, 1.284 mmol, 85.8% yield). ESIMS found for C₁₂H₁₅BrFNO m/z 289.0 (M+H).

2-(3-bromo-5-fluorophenoxy)-N,N-dimethylethan-1-amine (LX): Yellow oil (364 mg, 1.389 mmol, 50.2% yield). ESIMS found for C₁₀H₁₃BrFNO m/z 263.9 (M+H).

Preparation of intermediate tert-butyl 4-(2-((5-bromopyridin-3-yl)amino)-2-oxoethyl)piperidine-1-carboxylate (LXII) is depicted below in Scheme 11

Step 1

To a solution of 2-(1-(tert-butoxycarbonyl)piperidin-4-yl)acetic acid (LXI) (3.4 g, 13.97 mmol) in DCM (10 mL) was added DMF (1 mL). The solution was cooled in ice-water to 0° C. Oxalyl chloride (1.835 mL, 20.96 mmol) was then added dropwise. The mixture was stirred for one hour at 25° C. The organic volatile was then removed under vacuum. The residue was dissolved in DCM (10 mL). DMAP (0.171 g, 1.397 mmol) and 5-bromopyridin-3-amine (XX) (2.418 g, 13.97 mmol) were added to the solution and cooled to 0° C. DIEA (4.88 ml, 27.9 mmol) was then added dropwise and the mixture was stirred for 2 hours at 25° C. The reaction was worked-up with DCM and saturated NaHCO₃. The product was purified by ISCO eluted with 0-100% EtOAc-Hexanes. The tert-butyl 4-(2-((5-bromopyridin-3-yl)amino)-2-oxoethyl)piperidine-1-carboxylate (LXII) (2.82 g, 7.08 mmol, 50.7% yield) was obtained as yellow oil. ESIMS found for C₁₇H₂₄BrN₃O₃ m/z 343.1 (M-56).

The following intermediate was prepared in accordance with the procedure described in the above Scheme 11.

N-(5-Bromopyridin-3-yl)-2-(dimethylamino)acetamide (LXIII): Yellow oil (528 mg, 2.05 mmol, 19.0% yield). ESIMS found for C₉H₁₂BrN₃O m/z 259.3 (M+H).

Preparation of tert-butyl (1-(6-chloropyrazin-2-yl)azetidin-3-yl)carbamate (LXVI) is depicted below in Scheme 12.

Step 1

To a solution of tert-butyl azetidin-3-ylcarbamate hydrochloride (LXIV) (2 g, 9.58 mmol) in dry DMF (19.2 mL) was added DIPEA (8.37 ml, 47.9 mmol). To this mixture was added 2,6-dichloropyrazine (LXV) (1.428 g, 9.58 mmol) and the reaction was stirred at 95° C. for 3 hours. The reaction was quenched with water (20 mL) and extracted with EtOAc. The organic layer was dried over anhydrous Na₂SO₄, filtered and concentrated. The residue was purified by silica gel column chromatography (40 g) (100% hexanes→hexanes:EtOAc 1:1) to yield tert-butyl (1-(6-chloropyrazin-2-yl)azetidin-3-yl)carbamate (LXVI) (2.2882 g, 8.04 mmol, 83.9% yield) as a white solid. ESIMS found for C₁₂H₁₇ClN₄O₂ m/z 285.1 (M+H).

Preparation of intermediate 4-(2-fluorophenyl)-3-nitropyridin-2-amine (LXX) is depicted below in Scheme 13.

Step 1

A solution of 4-bromo-3-nitropyridin-2-amine (LXVII) (5.00 g, 22.9 mmol, 1.00 eq), (2-fluorophenyl)boronic acid (LXVIII) (3.82 g, 27.5 mmol, 1.20 eq), Pd(PPh₃)₄ (1.32 g, 1.14 mmol, 0.05 eq), and Na₂CO₃ (4.85 g, 45.8 mmol, 2 eq) in a mixture of toluene (25 mL), H₂O (9 mL) and EtOH (6 mL) was stirred at 75° C. for 15 h under nitrogen atmosphere. The reaction mixture was the washed with brine (50 mL) and dried over anhydrous Na₂SO₄, filtered and concentrated in vacuo. The resultant residue was purified by chromatography on silica gel (PE:EtOAc=3:1) to give 4-(2-fluorophenyl)-3-nitropyridin-2-amine (LXIX) (4.0 g, 17.15 mmol, 74.9%) as a yellow solid. ¹H NMR (CDCl₃, 400 MHz) δ ppm 6.29 (brs, 2H), 6.68 (d, J=4.8 Hz, 1H), 7.14 (t, J=5.2 Hz, 1H), 7.23-7.50 (m, 3H), 8.32 (d, J=4.8 Hz, 1H); ESIMS found C₁₁H₈FN₃O₂ m/z 234.2 (M+H).

Step 2

A mixture of 4-(2-fluorophenyl)-3-nitropyridin-2-amine (LXIX) (2.8 g, 12.0 mmol, 1 eq) and Pd/C (0.2 g) in MeOH (200 mL) was stirred under 50 psi of H₂ at room temperature overnight. The reaction was monitored by TLC. The mixture was filtered and the filtrate was concentrated in vacuo to produce 4-(2-fluorophenyl)pyridine-2,3-diamine (LXX) (1.55 g, 7.63 mmol, 63.6% yield) as a black solid. ¹H NMR (CDCl₃, 400 MHz) δ ppm 3.38 (brs, 2H), 4.40 (brs, 2H), 6.64 (d, J=4.8 Hz, 1H), 7.11-7.53 (m, 4H), 7.72 (d, J=4.8 Hz, 1H); ESIMS found C₁₁H₁₀FN₃ m/z 204.2 (M+H).

The following intermediates were prepared in accordance with the procedure described in the above Scheme 13.

4-(3-Fluorophenyl)pyridine-2,3-diamine (LXXI): Grey solid, (1.55 g, 7.63 mmol, 86.0% yield). ¹H NMR (CDCl₃, 400 MHz) δ ppm 3.50 (brs, 2H), 4.36 (brs, 2H), 6.63 (d, J=3.6 Hz, 1H), 7.3-7.37 (m, 3H), 7.47 (d, J=6 Hz, 1H), 7.72 (d, J=3.6 Hz, 1H); ESIMS found C₁₁H₁₀FN₃ m/z 204.2 (M+H).

4-(4-Fluorophenyl)pyridine-2,3-diamine (LXXII): Grey solid, (1.55 g, 7.63 mmol, 60.0% yield). ¹H NMR (CDCl₃, 400 MHz) δ ppm 3.46 (brs, 2H), 4.36 (brs, 2H), 6.62 (s, 1H), 7.19 (s, 2H), 7.43 (s, 2H), 7.70 (d, J=3.2 Hz, 1H); ESIMS found C₁₁H₁₀FN₃ m/z 204.1 (M+H).

4-(4-Fluorophenyl)pyridine-2,3-diamine (LXXIII): Grey solid, (1.55 g, 7.63 mmol, 60.0% yield). ¹H NMR (CDCl₃, 400 MHz) δ ppm 3.46 (brs, 2H), 4.36 (brs, 2H), 6.62 (s, 1H), 7.19 (s, 2H), 7.43 (s, 2H), 7.70 (d, J=3.2 Hz, 1H); ESIMS found C₁₁H₁₀FN₃ m/z 204.1 (M+H).

4-(Thiophen-3-yl)pyridine-2,3-diamine (LXXIV): Yellow solid, (1.9 g, 9.94 mmol, 84.9% yield). ¹H NMR (CDCl₃, 400 MHz) δ ppm 3.80 (brs, 2H), 4.34 (brs, 2H), 6.77 (s, 1H), 7.18 (s, 1H), 7.27 (s, 2H), 7.44 (s, 1H), 7.68 (s, 1H); ESIMS found C₉H₉N₃S m/z 192.2 (M+H).

4-(Furan-3-yl)pyridine-2,3-diamine (LXXV): Black solid, (1.9 g, 10.84 mmol, 89.0% yield). ¹H NMR (CDCl₃, 400 MHz) δ ppm 3.64 (brs, 2H), 4.32 (brs, 2H), 6.65 (s, 1H), 6.69 (d, J=4.8 Hz, 1H), 7.58 (s, 1H), 7.67 (d, J=4.8 Hz, 1H), 7.71 (s, 1H); ESIMS found C₉H₉N₃O m/z 176.3 (M+H).

4-(Thiophen-2-yl)pyridine-2,3-diamine (LXXVI): Yellow solid, (0.90 g, 4.71 mmol, 96.5% yield). ¹H NMR (CDCl₃, 400 MHz) δ ppm 3.63 (brs, 2H), 4.35 (brs, 2H), 6.71 (s, 1H), 7.27 (s, 1H), 7.45 (s, 1H), 7.49 (s, 1H), 7.69 (s, 1H); ESIMS found C₉H₉N₃S m/z 192.1 (M+H).

3-(2,3-Diaminopyridin-4-yl)-5-fluorophenol (LXXVII): White solid (303 mg, 1.38 mmol, 84.4% yield). ESIMS found for C₁₁H₁₀FN₃O m/z 220.1 (M+H).

4-(3-Fluoro-5-methoxyphenyl)pyridine-2,3-diamine (LXXVIII): White solid (404 mg, 1.73 mmol, 75.1% yield). ESIMS found for C₁₂H₁₂FN₃O m/z 234.1 (M+H).

4-(3-Fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)pyridine-2,3-diamine (LXXIX): Black oil (368 mg, 1.163 mmol, 95.0% yield). ESIMS found for C₁₇H₂₁FN₄O m/z 317.1 (M+H).

4-(3-(2-(Dimethylamino)ethoxy)-5-fluorophenyl)pyridine-2,3-diamine (LXXX): Black oil (352 mg, 1.212 mmol, 83.6% yield). ESIMS found for C₁₅H₁₉FN₄O m/z 291.1 (M+H).

Preparation of intermediate 3-nitro-[4,4′-bipyridin]-2-amine (LXXXIV) is depicted below in Scheme 14.

Step 1

To a solution of 4-chloro-3-nitropyridin-2-amine (LXXXI) (5.00 g, 28.9 mmol, 1.00 eq) and 4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridine (LXXXII) (7.1 g, 34.6 mmol, 1.20 eq) in a mixture of toluene (30 mL), H₂O (18 mL) and EtOH (6 mL) was added Pd(PPh₃)₄ (1.0 g, 0.87 mmol, 0.03 eq) and Na₂CO₃ (6.1 g, 57.6 mmol, 2 eq). The mixture was stirred at 75° C. for 15 h under a nitrogen atmosphere. The reaction mixture was then poured into brine (100 mL) and extracted with EtOAc (30 mL×3), the combined organic layers were dried over anhydrous Na₂SO₄, filtered and concentrated in vacuo. The resultant residue was purified by chromatography on silica gel (PE:EtOAc=5:1→1:1) to afford 3-nitro-[4,4′-bipyridin]-2-amine (LXXXIII) (1.80 g, 8.33 mmol, 28.8% yield) as a yellow solid. ESIMS found C₁₀H₈N₄O₂ m/z 217.1 (M+H).

Step 2

To a solution of 3-nitro-[4,4′-bipyridin]-2-amine (LXXXIII) (1.80 g, 8.33 mol, 1 eq) in MeOH (50 mL) was added Pd/C (0.5 g) under a nitrogen atmosphere. The mixture was stirred under 50 psi of H₂ for 6 h at room temperature. Then the mixture was filtered through a Celite pad and the filtrate was concentrated in vacuo to afford [4,4′-bipyridine]-2,3-diamine (LXXXIV) (1.4 g, 7.52 mmol, 90.4% yield) as a black solid. ESIMS found C₁₀H₁₀N₄ m/z 186.0 (M+H).

Preparation of intermediate 3-nitro-[4,4′-bipyridin]-2-amine (LXXXVII) is depicted below in Scheme 15.

Step 1

A solution of 4-chloro-3-nitropyridin-2-amine (LXXXI) (5.00 g, 28.9 mmol, 1.00 eq), 2-(tributylstannyl)pyridine (LXXXV) (15.9 g, 43.4 mmol, 1.50 eq), and Pd(PPh₃)₂Cl₂ (1.05 g, 1.44 mmol, 0.05 eq) in a mixture of toluene (25 mL) and H₂O (9 mL) was stirred at 75° C. for 16 h under a nitrogen atmosphere. The reaction mixture was then poured into brine (80 mL) and extracted with EtOAc (30 mL×3). The combined organic layers were dried over anhydrous Na₂SO₄, filtered and concentrated in vacuo. The resultant residue was purified by chromatography on silica gel (PE:EtOAc=5:1→1:1) to afford 3′-nitro-[2,4′-bipyridin]-2′-amine (LXXXVI) (1.6 g, 7.40 mmol, 25.6% yield) as a yellow solid. ESIMS found C₁₀H₈N₄O₂ m/z 217.1 (M+H).

Step 2

To a solution of compound 3′-nitro-[2,4′-bipyridin]-2′-amine (LXXXVI) (1.6 g, 7.4 mmol, 1.0 eq) in MeOH (50 mL) was added Pd/C (0.5 g) under a nitrogen atmosphere. The mixture was stirred under 50 psi of H₂ for 6 h at room temperature. The mixture was then filtered and concentrated in vacuo to afford [2,4′-bipyridine]-2′,3′-diamine (LXXXVII) (1.1 g, 5.91 mmol, 79.8%) as a black solid. ESIMS found C₁₀H₁₀N₄ m/z 186.1 (M+H).

Preparation of intermediate 4-(4-methylpiperazin-1-yl)pyridine-2,3-diamine (LXXXIX) is depicted below in Scheme 16.

Step 1

A solution of 4-bromo-3-nitropyridin-2-amine (LXVII) (2.50 g, 11.47 mmol) in 1-methylpiperazine (4 mL, 34.5 mmol) was heated at 140° C. overnight. The reaction was poured into an EtOAc/H₂O mixture; the organic layer was separated, dried over MgSO₄ and concentrated under vacuum. The crude product was purified on a silica gel column (100% CHCl₃→3:97 MeOH(7N NH₃):CHCl₃) to give 4-(4-methylpiperazin-1-yl)-3-nitropyridin-2-amine (LXXXVIII) as a yellow solid (1.80 g, 7.59 mmol, 66.1% yield). ¹H NMR (CDCl₃, 400 MHz) δ ppm 2.36 (s, 3H), 2.54 (t, J=4.8 Hz, 4H), 3.25 (t, J=5 Hz, 4H), 6.18 (s, 2H), 6.22 (d, J=6 Hz, 1H), 7.85 (d, J=6 Hz, 1H); ESIMS found C₁₀H₁₅N₅O₂ m/z 238.0 (M+H).

Step 2

To a solution of 4-(4-methylpiperazin-1-yl)-3-nitropyridin-2-amine (LXXXVIII) (1.80 g, 7.59 mmol) in MeOH (52 mL) was added 10% Pd/C (0.5 g). The solution was purged with hydrogen and stirred at room temperature under hydrogen for 4 h. The suspension was filtered through Celite® and the concentrated under vacuum to produce 4-(4-methylpiperazin-1-yl)pyridine-2,3-diamine (LXXXIX) as black solid (1.4 g, 6.75 mmol, 89.0% yield). ¹H NMR (CDCl₃, 400 MHz): δ ppm 2.38 (s, 3H), 2.60 (brs, 4H), 2.99 (s, 4H), 3.49 (brs, 2H), 4.12 (brs, 2H), 6.52 (d, J=5.6 Hz, 1H), 7.64 (d, J=5.6 Hz, 1H); ESIMS found C₁₀H₁₇N₅ m/z 208.1 (M+H).

The following intermediate was prepared in accordance with the procedure described in the above Scheme 16.

4-(Piperidin-1-yl)pyridine-2,3-diamine (XC): Black solid, (2.40 g, 12.48 mmol, 92.5% yield). ¹H NMR (CDCl₃, 400 MHz): δ ppm 1.61 (brs, 2H), 1.73 (s, 4H), 2.88 (s, 4H), 3.48 (brs, 2H), 4.13 (brs, 2H), 6.50 (d, J=5.2 Hz, 1H), 7.63 (d, J=4.8 Hz, 1H); ESIMS found C₁₀H₁₆N₄ m/z 193.1 (M+H).

Preparation of intermediate 4-(2-fluorophenyl)-3-nitropyridin-2-amine (XCIII) is depicted below in Scheme 17.

Step 1

4-Chloro-3-nitro-pyridin-2-amine (LXXXI) (3.00 g, 17.3 mmol, 1.0 eq) and 4-methyl-1H-imidazole (XCI) (2.84 g, 34.6 mmol, 2.0 eq) were taken up into a microwave tube in DMF (20 mL). The sealed tube was heated at 130° C. for 30 min under microwave. TLC showed the starting material was consumed, LC/MS showed the desired product was found. 10% NH₄Cl (60 mL) were added. The aqueous layer was extracted with DCM (2×100 mL). The combined organic layers were washed with brine (100 mL), dried over Na₂SO₄, concentrated in vacuum. The residue was purified by chromatography on silica gel (PE:THF=1:1) to give the product 4-(4-methylimidazol-1-yl)-3-nitro-pyridin-2-amine (XCII) (1.20 g, 5.47 mmol, 31.6% yield) as a yellow solid. ¹H NMR (CDCl₃, 400 MHz) δ ppm 2.30 (d, J=0.75 Hz, 3H), 5.96-6.26 (m, 2H), 6.68 (d, J=5.02 Hz, 1H), 6.72-6.75 (m, 1H), 7.57 (d, J=1.0 Hz, 1H), 8.32 (d, J=5.14 Hz, 1H); ESIMS found C₉H₉N₅O₂ m/z 219.1 (M+H).

Step 2

To a solution of 4-(4-methylimidazol-1-yl)-3-nitro-pyridin-2-amine (XCII) (900.0 mg, 4.11 mmol, 1.0 eq) in MeOH (50 mL) was added Pd/C (100.0 mg, 4.11 mmol, 1.0 eq) at room temperature. The mixture was stirred at 20° C. under H₂ for 2 hr. TLC (DCM:MeOH=20:1) showed that starting the material was consumed completely. The mixture was filtered and concentrated to afford 4-(4-methyl-1H-imidazol-1-yl)pyridine-2,3-diamine (XCIII) (750.0 mg, 3.96 mmol, 96.4% yield) as a brown solid. ¹H NMR (CDCl₃, 400 MHz) δ ppm 2.17 (s, 3H), 4.58 (brs, 2H), 5.85 (brs, 2H), 6.36 (d, J=5.4 Hz, 1H), 7.06 (s, 1H), 7.34 (s, 1H), 7.34 (s, 1H), 7.69 (d, J=0.88 Hz, 1H); ESIMS found C₉H₁₁N₅ m/z 190.1 (M+H).

Preparation of intermediate 4-(5-fluorothiophen-2-yl)pyridine-2,3-diamine (XCVI) is depicted below in Scheme 18.

Step 1

A solution of 2-chloro-3-nitro-pyridin-4-amine (LXXXI) (1.5 g, 8.64 mmol), 2-(5-fluorothiophen-2-yl)-4,4,5,5-tetramethyl-1,3,2-dioxaborolane (XCIV) (2.96 g, 12.96 mmol), Pd(dppf)Cl₂ (632 mg, 0.86 mmol) and Cs₂CO₃ (5.63 g, 17.29 mmol) in dioxane (30 mL) and H₂O (5 mL) was de-gassed and then heated to 100° C. under N² for 3 h. TLC (PE:EtOAc=1:1) showed the starting material was consumed completely. The mixture was concentrated in vacuum to give a residue, which was purified by column chromatography to afford 4-(5-fluorothiophen-2-yl)-3-nitropyridin-2-amine (XCV) (800 mg, 3.34 mmol, 38.7% yield). ESIMS found C₉H₆FN₃O₂S m/z 240.1 (M+H).

Step 2

A solution of 4-(5-fluorothiophen-2-yl)-3-nitropyridin-2-amine (XCV) (700 mg, 2.93 mmol), Fe (817 mg, 14.63 mmol) and NH₄Cl (939 mg, 17.56 mmol) in EtOH (18 mL) and H₂O (6 mL) was heated to 80° C. for 2 h. TLC (PE:EtOAc=1:1) showed the starting material was consumed completely. The mixture was filtered, washed with HCl/EtOH, concentrated, basified to pH=7˜8, extracted with EtOAc and H₂O, the organic layer was concentrated to give 4-(5-fluorothiophen-2-yl)pyridine-2,3-diamine (XCVI) (550 mg, 2.63 mmol, 89.7% yield). ESIMS found C₉H₈FN₃S m/z 210.0 (M+H).

The following intermediates were prepared in accordance with the procedure described in the above Scheme 18.

4-(5-Methylthiophen-2-yl)pyridine-2,3-diamine (XCVII): Brown solid, (1.20 g, 5.85 mmol, 86.0% yield). ¹H NMR (CD₃OD, 400 MHz) δ ppm 2.54 (s, 3H), 6.63 (d, J=4.8 Hz, 1H), 6.85 (s, 1H), 7.12 (s, 1H), 7.38 (d, J=5.2 Hz, 1H); ESIMS found C₁₀H₁₁N₃S m/z 206.2 (M+H).

1-(5-(2,3-Diaminopyridin-4-yl)thiophen-2-yl)ethan-1-one (XCVIII): Brown solid, (500 mg, 2.14 mmol, 56.4% yield). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 2.55 (s, 3H), 4.89 (brs, 2H), 5.80 (brs, 2H), 6.52 (d, J=5.2 Hz, 1H), 7.33 (d, J=5.2 Hz, 1H), 7.46 (d, J=4.0 Hz, 1H), 7.96 (d, J=4.0 Hz, 1H); ESIMS found C₁₁H₁₁N₃OS m/z 234 (M+H).

Preparation of intermediate 4-(3-((2-(dimethylamino)ethyl)amino)-5-fluorophenyl)pyridine-2,3-diamine (CIII) is depicted below in Scheme 19.

Step 1

A solution of 3-bromo-5-fluorobenzaldehyde (XCIX) (20.0 g, 98.2 mmol, 1.0 eq) in MeOH (1.8 L) was added N¹,N¹-dimethylethane-1,2-diamine (21.5 mL, 196.4 mmol, 2.0 eq). The pH was adjusted to 6 using HOAc and stirred for 1 h. NaCNBH₃ (8.6 g, 137.5 mmol, 1.4 eq) was added and stirred at room temperature overnight. The MeOH was removed under vacuum and the residue was partitioned between CHCl₃ and saturated aqueous NaHCO₃. The organic layer was dried over MgSO₄ and evaporated under vacuum. The crude product was purified on a silica gel column (100% CHCl₃→3:97 MeOH[7N NH₃]:CHCl₃) to produce N¹-(3-bromo-5-fluorophenyl)-N²,N²-dimethylethane-1,2-diamine (C) as a yellow oil (13.0 g, 49.9 mmol, 51% yield). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 1.28 (s, 6H), 2.39 (t, J=4 Hz, 2H), 3.07 (q, J=6 Hz, 2H), 6.10 (t, J=5 Hz, 1H), 6.38 (td, J=12 Hz, J=2 Hz, 1H), 6.51 (td, J=8.6 Hz, J=2 Hz, 1H), 6.61 (t, J=2 Hz, 1H); ESIMS found C₁₀H₁₄BrFN₂ m/z 261.0 (M+H).

Step 2

A solution of N¹-(3-bromo-5-fluorophenyl)-N²,N²-dimethylethane-1,2-diamine (C) (13.0 g, 49.9 mmol, 1.0 eq), bis(pinacolato)diboron (12.6 g, 59.9 mmol, 1.2 eq), KOAc (12.1 g, 124.3 mmol, 2.5 eq) and dioxane (600 mL) was purged with argon. Pd(dppf)Cl₂ (2.0 g, 2.47 mmol, 0.05 eq) was added to the reaction and purged again with argon. The solution was heated at 90° C. for 2 h. Once TLC showed the disappearance of (C), the solution was cooled to room temperature and then concentrated under reduced pressure to produce crude N¹-(3-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-N²,N²-dimethylethane-1,2-diamine (CI) (7.4 g, 24.0 mmol, 48.2% yield).

Step 3

To a solution of N¹-(3-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-N²,N²-dimethylethane-1,2-diamine (CI) (5.0 g, 16.22 mmol, 1.2 eq) in water (25 mL) was added K₂CO₃ (448 mg, 3.24 mmol, 2.0 eq), 4-bromo-3-nitropyridin-2-amine (LXVII) (3.5 g, 16.22 mmol, 1.0 eq) and Pd(dppf)Cl₂ (1.0 g, 81.0 μmol, 0.05 eq). The solution was purged with argon and heated at 100° C. for 48 h. The solution was cooled to room temperature and then concentrated under reduced pressure. The residue was purified on a silica gel column (100% CHCl₃→2:98 MeOH[7N NH₃]:CHCl₃) to give N¹-(3-(3-amino-2-nitropyridin-4-yl)-5-fluorophenyl)-N²,N²-dimethylethane-1,2-diamine (CII) as a yellow amorphous solid (4.5 g, 14.1 mmol, 86.9% yield). ESIMS found for C₁₅H₁₈FN₅O₂ m/z 220.1 (M+H).

Step 4

To a solution of N¹-(3-(3-amino-2-nitropyridin-4-yl)-5-fluorophenyl)-N²,N²-dimethylethane-1,2-diamine (CII) (4.50 g, 10.43 mmol, 1.0 eq) in MeOH (15 mL) was added 10% Pd/C (540 mg, 15% by wt). The solution was purged with hydrogen and stirred for 48 h at room temperature under hydrogen (15 psi). The suspension was filtered through Celite® and concentrated under vacuum and purified by silica gel chromatography (MeOH:DCM=10:1) to produce 4-(3-((2-(dimethylamino)ethyl)amino)-5-fluorophenyl)pyridine-2,3-diamine (CIII) as a tan solid (750.0 mg, 2.59 mmol, 24.9% yield). ¹H NMR (CD₃OD, 400 MHz) δ ppm 2.32 (s, 6H), 2.60 (t, J=6.8 Hz, 2H), 3.26 (t, J=6.8 Hz, 2H), 6.34-6.43 (m, 2H), 6.47 (d, J=5.6 Hz, 1H), 7.58 (s, 1H), 7.75 (s, 1H); ESIMS found C₁₅H₂₀FN₅ m/z 290.1 (M+H).

Preparation of intermediate N-(3-(2,3-diaminopyridin-4-yl)-5-fluorobenzyl)methanesulfonamide (CIX) is depicted below in Scheme 20.

Step 1

A solution of 3-bromo-5-fluorobenzonitrile (CIV) (44.0 g, 220.0 mmol, 1.0 eq) was dissolved in THF (30 mL). BH₃-Me₂S (33.43 g, 440.0 mmol, 2.0 eq) was added to the solution at 20° C. Then it was stirred at 80° C. for 2 h, HCl (6 N, 100 mL) was added to the mixture slowly at 20° C. The mixture was stirred at 80° C. for 1 h, then it was washed with EtOAc (300 ml). The water phase was basified with 50% aqueous NaOH and it was extracted with EtOAc (300 mL×3). The combined organic layers were dried over anhydrous Na₂SO₄ and concentrated in vacuo to produce (3-bromo-5-fluoro-phenyl)methanamine (CV) (24.0 g, 117.62 mmol, 53.5% yield). ¹H NMR (CDCl₃, 300 MHz) 3.86 (s, 2H), 7.01 (d, J=8 Hz, 1H), 7.12 (d, J=8 Hz, 1H), 7.28 (s, 1H); ESIMS found C₇H₇BrFN m/z 203.9 (Br⁷⁹M+H).

Step 2

A solution of (3-bromo-5-fluoro-phenyl)methanamine (CV) (23.0 g, 112.7 mmol, 1.0 eq) was dissolved in DCM (15 mL), TEA (34.22 g, 338.2 mmol, 3.0 eq) was added to the mixture. Then MsCl (13.44 g, 117.3 mmol, 1.04 eq) was added slowly to the solution at 0° C. It was stirred at 0-30° C. for 2 h. The reaction was washed with water and extracted with EtOAc. The combined organic layers were dried over anhydrous Na₂SO₄ and concentrated to give N-(3-bromo-5-fluorobenzyl)methanesulfonamide (CVI) (34.0 g, 102.44 mmol, 90.9% yield, 85% purity) as an oil. ¹H NMR (CDCl₃, 300 MHz) 2.88 (s, 3H), 4.24 (d, J=4.5 Hz, 2H), 6.99 (d, J=9 Hz, 1H), 7.13 (dt, J=8.1 Hz, J=2 Hz, 1H), 7.25 (s, 1H); ESIMS found C₈H₉BrFNO₂S m/z 282.0 (Br⁷⁹M+H).

Step 3

A solution of N-(3-bromo-5-fluorobenzyl)methanesulfonamide (CVI) (34.0 g, 102.4 mmol, 1.0 eq) and 4,4,5,5-tetramethyl-2-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-1,3,2-dioxaborolane (52.02 g, 204.9 mmol, 2.0 eq), KOAc (20.11 g, 204.9 mmol, 2.0 eq) was dissolved in dioxane (20 mL). Then Pd(dppf)Cl₂ (7.60 g, 10.2 mmol, 0.1 eq) was added to the mixture. It was stirred at 90° C. for 2 h. Then the solvent was removed to get the residue which was purified by silica gel column (PE:EtOAc=10:1→100% EtOAc) to get N-(3-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)benzyl)methanesulfonamide (CVII) (30.0 g, crude). ¹H NMR (CDCl₃, 400 MHz) 1.37 (s, 12H), 2.92 (s, 3H), 4.34 (d, J=6.3 Hz, 2H), 7.19 (dt, J=9.3 Hz, J=2.1 Hz, 1H), 7.44 (dd, J=8.7 Hz, J=2.4 Hz, 1H), 7.54 (s, 1H); ESIMS found C₁₄H₂₁BFNO₄S m/z 330.1 (M+H).

Step 4

A solution of N-(3-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl) benzyl)methanesulfonamide (CVII) (6.83 g, 20.75 mmol, 1.2 eq), 4-chloro-3-nitropyridin-2-amine (LXXXI) (3.0 g, 17.29 mmol, 1.0 eq), Na₂CO₃ (6.41 g, 60.52 mmol) and Pd(dppf)Cl₂ (641.27 mg, 864.50 μmol) in dioxane (40 mL) and H₂O (8 mL) was de-gassed and then heated to 80° C. overnight under N₂. TLC (PE:EtOAc=1:1) showed the starting material was consumed completely. The reaction mixture was poured into H₂O (300 mL). The mixture was extracted with EtOAc (3×250 mL). The organic phase was washed with saturated brine (300 mL), dried over anhydrous NaSO₄, concentrated in vacuum to give a residue. The crude product was purified by silica gel chromatography (PE:EtOAc=10:1) to give N-(3-(2-amino-3-nitropyridin-4-yl)-5-fluorobenzyl) methanesulfonamide (CVIII) (2.2 g, 6.46 mmol, 37.4% yield) as brown solid. ESIMS found C₁₃H₁₃FN₄O₄S m/z 341.1 (M+H).

Step 5

A solution of N-[[3-(4-amino-3-nitro-2-pyridyl)-5-fluoro-phenyl]methyl]methanesulfonamide (CVIII) (2.2 g, 6.46 mmol, 1.0 eq), Fe (1.44 g, 25.84 mmol, 4.0 eq) and NH₄Cl (2.8 g, 51.68 mmol, 8.0 eq) was dissolved in MeOH (30 mL). The mixture was stirred at 80° C. for 16 h. The mixture was cooled to room temperature and concentrated in reduced pressure at 60° C. The combined organic phase was washed with saturated brine (100 mL×2), dried over anhydrous Na₂SO₄, filtered and concentrated in vacuo to get crude N-(3-(3,4-diaminopyridin-2-yl)-5-fluorobenzyl) methanesulfonamide (CIX) (1.60 g, 5.16 mmol, 79.8% yield) as brown solid. ¹H NMR (CD₃OD, 400 MHz) 2.96 (s, 3H), 3.37 (s, 2H), 6.53 (d, J=4 Hz, 1H), 7.13 (d, J=8.8 Hz, 1H), 7.20 (d, J=9.2 Hz, 1H), 7.31 (s, 1H), 7.44 (d, J=4 Hz, 1H); ESIMS found C₁₃H₁₅FN₄O₂S m/z 311.1 (M+H).

Example 1

Preparation of N-(5-(3-(7-(3-((2-(Dimethylamino)ethyl)amino)-5-fluorphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)pentanamide (432) is depicted below in Scheme 21.

Step 1

A solution of 5-bromo-1-(tetrahydro-pyran-2-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl]-carbaldehyde (XIX) (872 mg, 2.81 mmol, 1.0 eq), bis(pinacolato)diboron (898 mg, 3.37 mmol,), and KOAc (824 mg, 8.4 mmol, 3.0 eq) in dry DMF (40 mL) was purged with argon. PdCl₂(dppf)₂ (120 mg, 0.14 mmol, 0.05) was added to the solution and purged again with argon. The solution was heated at 90° C. for 2 h under argon and cooled to the room temperature. The reaction mixture was diluted in water (30 mL) and extracted with DCM (30 mL×3). The combined organic layers were dried over Na₂SO₄, filtered and concentrated in vacuo to give 1-(tetrahydro-2H-pyran-2-yl)-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-1H-pyrazolo [3,4-b]pyridine-3-carbaldehyde (CX) which was used for the next step without further purification.

Step 2

A solution of 1-(tetrahydro-2H-pyran-2-yl)-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-1H-pyrazolo [3,4-b]pyridine-3-carbaldehyde (CX) (1.0 g, 2.80 mmol, 1.0 eq), N-(5-bromopyridin-3-yl)pentanamide (XXVI) (720.0 mg, 2.80 mmol, 1.0 eq), Pd(dppf)Cl₂ (144 mg, 0.196 mmol, 0.07 eq) and Na₂CO₃ (0.89 g, 8.40 mmol, 3.0 eq) in a mixed solvent of 1,2-dimethoxyethane (30 mL) and H₂O (5 mL) was refluxed for 3 h under a nitrogen atmosphere. The reaction mixture was diluted in water (30 mL) and extracted with DCM (30 mL×3). The combined organic layers were dried over Na₂SO₄, filtered and concentrated in vacuo, the resultant residue was purified by flash chromatography on silica gel eluting with 100% EtOAc to give the N-(5-(3-formyl-1-(tetrahydro-2H-pyran-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)pentanamide (CXI) (960 mg, 2.36 mmol, 84.1% for 2 steps) as a yellow solid. ¹H NMR (CDCl₃, 400 MHz) δ ppm 1.00 (t, J=7.6 Hz, 3H), 1.47 (sxt, J=7.6 Hz, 2H), 1.68-1.84 (m, 3H), 1.89 (quin, J=8.8 Hz, 2H), 2.04-2.17 (m, 1H), 2.18-2.31 (m, 1H), 2.50 (t, J=7.2 Hz, 2H), 2.62-2.78 (m, 1H), 3.91 (t, J=10.4 Hz, 1H), 4.21 (d, J=8 Hz, 1H), 6.32 (d, J=10.4 Hz, 1H), 7.39 (s, 1H), 8.52 (s, 1H), 8.64 (s, 1H), 8.79 (s, 1H), 8.89 (d, J=1.6 Hz, 1H), 10.28 (s, 1H); ESIMS found C₂₂H₂₅N₅O₃ m/z 408.3 (M+H).

Step 3

A solution of N-(5-(3-formyl-1-(tetrahydro-2H-pyran-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)pentanamide (CXI) (100.0 mg, 0.25 mmol, 1.0 eq), 4-(3-(2-(dimethylamino)ethylamino)-5-fluorophenyl)pyridine-2,3-diamine (CIII) (71.0 mg, 0.25 mmol, 1.0 eq) and Na₂S₂O₅ (56.3 mg, 0.30 mmol, 1.2 eq) in DMF (2 mL) was stirred at 120° C. for 24 h. LC/MS showed the starting material was consumed. Water (5 mL) was added in dropwise and the mixture was filtered. The filtrate was washed by MeOH (0.5 mL) and used for directly for next step without further purification.

Step 4

Crude N-(5-(3-(7-(3-(2-(dimethylamino)ethylamino)-5-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1-(tetrahydro-2H-pyran-2-yl)-1H-pyrazolo[3,4-b] pyridin-5-yl)pyridin-3-yl)pentanamide (CXII) was mixed with in HCl/EtOAc (15 mL) was stirred at 10-35° C. for 12 h. LC/MS showed the starting material was consumed. The mixture was concentrated to give a residue. The residue was purified by pre-HPLC (HCl) to give N-(5-(3-(7-(3-((2-(Dimethylamino)ethyl)amino)-5-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)pentanamide (432) (29.6 mg, 0.05 mmol, 24.4% yield for 2 steps) as a white solid. ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 0.92 (t, J=7.34 Hz, 3H), 1.36 (sxt, J=7.36 Hz, 2H), 1.62 (quin, J=7.47 Hz, 2H), 2.43 (t, J=7.40 Hz, 2H), 2.82 (d, J=3.76 Hz, 6H), 3.22-3.30 (m, 3H), 6.60 (d, J=11.17 Hz, 1H), 7.48 (brs, 2H), 7.59 (d, J=5.14 Hz, 1H), 8.45 (d, J=5.15 Hz, 1H), 8.55 (s, 1H), 8.87 (s, 1H), 8.99 (d, J=2.01 Hz, 2H), 9.11 (d, J=2.13 Hz, 1H), 10.06 (brs, 1H), 10.58 (brs, 1H), 14.61 (brs, 1H); ESIMS found for C₃₂H₃₃FN₁₀O m/z 593.3 (M+1).

The following compound was prepared in accordance with the procedure described in the above Example 1.

5-(3-(7-(3-Fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo [3,4-b]pyridin-5-yl)-N,N-dimethylpyridin-3-amine 5.

White solid (6.0 mg, 0.01 mmol). ¹H NMR (CD₃OD, 400 MHz) δ ppm 3.27 (s, 6H), 7.47 (td, J=8.63, 2.70 Hz, 1H), 7.75 (td, J=8.06, 5.83 Hz, 1H), 7.80-7.87 (m, 2H), 7.90 (d, J=6.27 Hz, 1H), 8.14 (s, 1H), 8.26 (d, J=2.64 Hz, 1H), 8.48 (s, 1H), 8.69 (d, J=6.27 Hz, 1H), 9.08 (d, J=2.01 Hz, 1H), 9.26 (d, J=2.13 Hz, 1H); ESIMS found for C₂₅H₁₉FN₈ m/z 451.3 (M+1).

1-(5-(3-(7-(3-Fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-N,N-dimethylmethanamine 10.

White solid (20.0 mg, 0.04 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 2.80 (d, J=4.64 Hz, 6H), 4.49 (d, J=4.89 Hz, 2H), 7.39 (td, J=8.50, 2.70 Hz, 1H), 7.65 (q, J=6.4 Hz, 1H), 7.71 (d, J=5.27 Hz, 1H), 8.20-8.28 (m, 1H), 8.41-8.52 (m, 1H), 8.48 (d, J=5.15 Hz, 1H), 8.73 (brs, 1H), 8.91 (s, 1H), 9.15 (d, J=2.13 Hz, 1H), 9.19 (d, J=2.13 Hz, 1H), 9.24 (s, 1H), 11.13 (brs, 1H), 14.67 (brs, 1H); ESIMS found for C₂₆H₂₁FN₈ m/z 465.1 (M+1).

5-(5-((3,3-Difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-3-(7-(3-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine 21.

White solid (19.0 mg, 0.04 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 2.20-2.32 (m, 2H), 2.77 (t, J=6.96 Hz, 2H), 2.96 (t, J=13.30 Hz, 2H), 3.80 (s, 2H), 7.36 (td, J=8.16, 2.51 Hz, 1H), 7.63 (q, J=6.64 Hz, 1H), 7.70 (d, J=3.51 Hz, 1H), 8.16 (s, 1H), 8.27-8.33 (m, 1H), 8.44 (d, J=5.15 Hz, 1H), 8.58 (dd, J=10.29, 1.63 Hz, 1H), 8.62 (d, J=1.76 Hz, 1H), 8.99 (d, J=1.88 Hz, 1H), 9.08 (d, J=2.13 Hz, 1H), 9.13 (d, J=1.63 Hz, 1H), 14.11 (brs, 1H), 14.47 (brs, 1H); ESIMS found for C₂₈H₂₁F₃N₈ m/z 527.2 (M+1).

N-(5-(3-(7-(4-Fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)pivalamide 28.

White solid (17.2 mg, 0.03 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.32 (s, 9H), 7.46 (t, J=8.91 Hz, 2H), 7.63 (d, J=5.14 Hz, 1H), 8.42 (d, J=5.14 Hz, 1H), 8.62 (dd, J=8.78, 5.40 Hz, 2H), 8.70 (s, 1H), 8.76 (d, J=1.88 Hz, 1H), 8.90 (d, J=2.13 Hz, 1H), 9.03-9.06 (m, 1H), 9.06 (s, 1H), 9.68 (s, 1H), 14.02 (s, 1H); ESIMS found for C₂₈H₂₃FN₈O m/z 507.1 (M+1).

3-(7-(4-Fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(pyrrolidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine 33.

White solid (9.0 mg, 0.02 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.86-1.99 (m, 2H), 2.01-2.13 (m, 2H), 3.11-3.23 (m, 2H), 3.42-3.53 (m, 2H), 4.62 (d, J=5.02 Hz, 2H), 7.47 (t, J=8.91 Hz, 2H), 7.65 (d, J=5.40 Hz, 1H), 8.39-8.54 (m, 3H), 9.03 (s, 1H), 9.05 (s, 1H), 9.18 (d, J=1.88 Hz, 2H), 9.32 (d, J=1.63 Hz, 1H), 11.76 (brs, 1H), 14.76 (brs, 1H); ESIMS found for C₂₈H₂₃FN₈ m/z 491.2 (M+1).

N-(5-(3-(7-(4-Fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclobutanecarboxamide 38.

White solid (22.0 mg, 0.04 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.80-1.92 (m, 1H), 1.94-2.08 (m, 1H), 2.15-2.26 (m, 2H), 2.27-2.39 (m, 2H), 3.39 (quin, J=8.31 Hz, 2H), 7.49 (t, J=8.91 Hz, 2H), 7.63 (d, J=5.27 Hz, 1H), 8.45 (d, J=5.27 Hz, 1H), 8.54 (brs, 2H), 8.90 (s, 1H), 8.96 (d, J=1.63 Hz, 1H), 8.99 (d, J=1.88 Hz, 1H), 9.07 (d, J=2.13 Hz, 1H), 9.10 (d, J=2.26 Hz, 1H), 10.73 (s, 1H), 14.65 (brs, 1H); ESIMS found for C₂₈H₂₁FN₈O m/z 505.2 (M+1).

3-(7-(4-Fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyrimidin-5-yl)-1H-pyrazolo[3,4-b]pyridine 44.

White solid (58.2 mg, 0.14 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 7.42 (t, J=8.85 Hz, 2H), 7.61 (d, J=5.27 Hz, 1H), 8.44 (d, J=5.15 Hz, 1H), 8.54 (brs, 2H), 9.12 (d, J=2.26 Hz, 1H), 9.19 (d, J=2.13 Hz, 1H), 9.30 (s, 1H), 9.34 (s, 2H), 14.59 (brs, 1H); ESIMS found for C₂₂H₁₃FN₈ m/z 409.0 (M+1).

5-(3-(7-(2-Fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo [3,4-b]pyridin-5-yl)pyridin-3-amine 45.

White solid (24.5 mg, 0.06 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 7.41-7.52 (m, 3H), 7.56-7.64 (m, 1H), 7.93-8.17 (m, 1H), 7.99 (s, 1H), 8.11 (d, J=1.76 Hz, 1H), 8.48-8.54 (m, 2H), 9.02 (d, J=4.89 Hz, 2H), 14.71 (brs, 1H); ESIMS found for C₂₃H₁₅FN₈ m/z 423.1 (M+1).

N-(5-(3-(7-(2-Fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-3,3-dimethylbutanamide 57.

White solid (27.0 mg, 0.05 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.08 (s, 9H), 2.30 (s, 2H), 7.38-7.49 (m, 3H), 7.52 (brs, 1H), 8.26 (brs, 1H), 8.45 (brs, 1H), 8.50 (brs, 1H), 8.67 (brs, 1H), 8.77 (brs, 1H), 8.97 (brs, 2H), 10.27 (s, 1H), 14.03 (brs, 1H), 14.52 (brs, 1H); ESIMS found for C₂₉H₂₅FN₈O m/z 521.3 (M+1).

N-(5-(3-(7-(2-Fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclopentanecarboxamide 61.

White solid (13.0 mg, 0.03 mmol). ¹H NMR (CD₃OD, 400 MHz) δ ppm 1.65-1.76 (m, 2H), 1.76-1.87 (m, 2H), 1.87-1.98 (m, 2H), 2.00-2.10 (m, 2H), 3.00 (quin, J=8.00 Hz, 1H), 7.43-7.56 (m, 2H), 7.73 (q, J=6.27 Hz, 1H), 7.81-7.90 (m, 2H), 8.70 (d, J=6.15 Hz, 1H), 9.03 (brs, 2H), 9.06 (d, J=1.88 Hz, 1H), 9.28 (d, J=1.88 Hz, 1H), 9.34 (brs, 1H); ESIMS found for C₂₉H₂₃FN₈O m/z 519.2 (M+1).

5-(Pyridin-3-yl)-3-(7-(pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine 68.

White solid (60.7 mg, 0.16 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 7.84 (brs, 1H), 7.96-8.16 (m, 2H), 8.55 (d, J=4.77 Hz, 1H), 8.81 (brs, 1H), 8.88 (brs, 1H), 8.92 (brs, 1H), 9.14 (s, 1H), 9.20 (brs, 1H), 9.24-9.41 (m, 3H), 9.88 (brs, 1H), 14.71 (brs, 1H); ESIMS found for C₂₂H₁₄N₈ m/z 391.0 (M+1).

N-Isopropyl-5-(3-(7-(pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-amine 75.

White solid (11.0 mg, 0.02 mmol). ¹H NMR (CD₃OD, 400 MHz) δ ppm 1.34 (d, J=6.40 Hz, 6H), 3.87 (spt, J=6.40 Hz, 1H), 7.89 (d, J=5.52 Hz, 1H), 8.02 (s, 1H), 8.09 (d, J=2.26 Hz, 1H), 8.33 (dd, J=7.97, 5.58 Hz, 1H), 8.42 (s, 1H), 8.67 (d, J=5.40 Hz, 1H), 9.00 (d, J=2.01 Hz, 1H), 9.05 (d, J=5.27 Hz, 1H), 9.21 (d, J=2.01 Hz, 1H), 9.39 (d, J=7.91 Hz, 1H), 9.84 (s, 1H); ESIMS found for C₂₅H₂₁N₉ m/z 448.2 (M+1).

5-(5-(Piperidin-1-ylmethyl)pyridin-3-yl)-3-(7-(pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine 78.

White solid (9.2 mg, 0.02 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.34-1.48 (m, 1H), 1.67-1.88 (m, 5H), 2.91-3.06 (m, 2H), 3.36-3.44 (m, 2H), 4.50 (s, 2H), 7.78 (d, J=5.02 Hz, 1H), 7.90 (d, J=3.64 Hz, 1H), 8.53 (d, J=5.02 Hz, 1H), 8.63 (brs, 1H), 8.82-8.89 (m, 2H), 9.07 (brs, 1H), 9.14 (d, J=2.13 Hz, 1H), 9.17 (d, J=2.26 Hz, 1H), 9.20 (d, J=2.13 Hz, 1H), 9.84 (brs, 1H), 10.41 (brs, 1H), 14.64 (s, 1H); ESIMS found for C₂₈H₂₅N₉ m/z 488.2 (M+1).

5-(4-Methylpyridin-3-yl)-3-(7-(pyridin-4-yl)-3H-imidazo[4,5-b] pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine 91.

White solid (21.0 mg, 0.05 mmol). ¹H NMR (CD₃OD, 400 MHz) δ ppm 2.71 (s, 3H), 7.97 (d, J=5.65 Hz, 1H), 8.18 (d, J=6.15 Hz, 1H), 8.71 (d, J=5.52 Hz, 1H), 8.80 (d, J=2.01 Hz, 1H), 8.85 (d, J=6.02 Hz, 1H), 8.94-9.00 (m, 3H), 9.08-9.15 (m, 3H); ESIMS found for C₂₃H₁₆N₈ m/z 405.2 (M+1).

2-Phenyl-N-(5-(3-(7-(pyridin-4-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide 95.

White solid (22.0 mg, 0.04 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 3.96 (s, 2H), 7.20-7.26 (m, 1H), 7.32 (t, J=7.34 Hz, 2H), 7.44 (d, J=7.40 Hz, 2H), 7.96 (d, J=5.14 Hz, 1H), 8.60 (d, J=5.27 Hz, 1H), 9.09 (s, 1H), 9.12-9.17 (m, 2H), 9.18 (s, 1H), 9.22 (brs, 3H), 9.26 (brs, 1H), 9.30 (s, 1H), 12.30 (brs, 1H); ESIMS found for C₃₀H₂₁N₉O m/z 524.2 (M+1).

N-(5-(3-(7-(Pyridin-4-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)butyramide 102.

White solid (14.0 mg, 0.03 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 0.97 (t, J=7.40 Hz, 3H), 1.70 (sxt, J=7.33 Hz, 2H), 2.48 (t, J=7.16 Hz, 2H), 7.93 (d, J=5.27 Hz, 1H), 8.59 (d, J=5.02 Hz, 1H), 8.84 (brs, 1H), 8.90 (brs, 1H), 8.93 (s, 1H), 9.01 (brs, 2H), 9.06-9.11 (m, 3H), 9.12 (d, J=1.63 Hz, 1H), 10.75 (brs, 1H), 14.71 (brs, 1H); ESIMS found for C₂₆H₂₁N₉O m/z 476.1 (M+1).

N-(5-(3-(7-(Pyridin-4-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclohexanecarboxamide 106.

White solid (20.0 mg, 0.04 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.19-1.36 (m, 3H), 1.40-1.53 (m, 2H), 1.61-1.70 (m, 1H), 1.73-1.83 (m, 2H), 1.93 (d, J=12.05 Hz, 2H), 2.51-2.58 (m, 1H), 7.93 (d, J=5.14 Hz, 1H), 8.57 (d, J=5.14 Hz, 1H), 9.02 (s, 1H), 9.03-9.08 (m, 2H), 9.08-9.20 (m, 6H), 11.18 (brs, 1H), 14.75 (brs, 1H); ESIMS found for C₂₉H₂₅N₉O m/z 516.1 (M+1).

N-((5-(3-(7-(Pyridin-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methyl)ethanamine 114.

White solid (32.0 mg, 0.07 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.30 (t, J=7.22 Hz, 3H), 2.97-3.08 (m, 2H), 4.29-4.38 (m, 2H), 7.56 (dd, J=7.03, 5.02 Hz, 1H), 8.11 (d, J=5.15 Hz, 1H), 8.14 (td, J=7.72, 1.38 Hz, 1H), 8.52 (d, J=5.02 Hz, 1H), 8.82 (brs, 1H), 8.86 (d, J=4.64 Hz, 1H), 8.90 (s, 1H), 9.16 (d, J=2.01 Hz, 1H), 9.20 (brs, 3H), 9.90 (brs, 2H), 14.75 (brs, 1H); ESIMS found for C₂₅H₂₁N₉ m/z 448.2 (M+1).

1-Cyclopentyl-N-((5-(3-(7-(pyridin-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methyl)methanamine 130.

White solid (33.0 mg, 0.07 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.22-1.34 (m, 2H), 1.46-1.64 (m, 4H), 1.75-1.86 (m, 2H), 2.28 (spt, J=7.76 Hz, 1H), 2.94-3.02 (m, 2H), 4.40 (t, J=5.40 Hz, 2H), 7.62 (dd, J=7.15, 5.40 Hz, 1H), 8.13 (d, J=5.27 Hz, 1H), 8.23 (td, J=7.84, 1.63 Hz, 1H), 8.57 (d, J=5.27 Hz, 1H), 8.91 (d, J=4.02 Hz, 1H), 8.98 (s, 2H), 9.12 (d, J=4.52 Hz, 1H), 9.19 (d, J=2.01 Hz, 1H), 9.24 (d, J=2.26 Hz, 1H), 9.31 (s, 1H), 9.59 (brs, 2H), 14.76 (brs, 1H); ESIMS found for C₂₉H₂₇N₉ m/z 502.3 (M+1).

N-(5-(3-(7-(Piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)propionamide 133.

White solid (23.0 mg, 0.05 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.14 (t, J=7.47 Hz, 3H), 1.76 (brs, 6H), 2.47 (q, J=7.64 Hz, 2H), 3.92-4.22 (m, 4H), 6.96 (d, J=7.53 Hz, 1H), 7.98 (brs, 1H), 8.75-8.90 (m, 4H), 9.08 (d, J=2.01 Hz, 1H), 10.84 (s, 1H), 14.67 (brs, 1H), 14.96 (s, 1H); ESIMS found for C₂₅H₂₅N₉O m/z 468.3 (M+1).

N,N-Dimethyl-5-(3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-amine 139.

White solid (31.0 mg, 0.07 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1H NMR (400 MHz, DMSO-d₆) δ 1.76 (brs, 6H), 3.17 (s, 6H), 4.25 (brs, 4H), 6.97 (d, J=7.65 Hz, 1H), 7.99 (brs, 1H), 8.08 (brs, 1H), 8.27 (d, J=2.64 Hz, 1H), 8.52 (s, 1H), 8.96 (d, J=2.01 Hz, 1H), 9.15 (d, J=2.13 Hz, 1H), 14.75 (brs, 1H); ESIMS found for C₂₄H₂₅N₉ m/z 440.2 (M+1).

N,N-Dimethyl-1-(5-(3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methanamine 145.

White solid (13.0 mg, 0.03 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.76 (brs, 6H), 2.79 (d, J=4.02 Hz, 6H), 4.44 (d, J=3.64 Hz, 2H), 6.97 (d, J=6.90 Hz, 1H), 7.98 (brs, 1H), 8.61 (s, 1H), 8.81 (d, J=1.63 Hz, 1H), 8.93 (brs, 1H), 9.09 (d, J=1.88 Hz, 1H), 9.12 (d, J=2.13 Hz, 1H), 11.13 (brs, 1H), 14.68 (brs, 1H), 14.98 (s, 1H); ESIMS found for C₂₅H₂₇N₉ m/z 454.2 (M+1).

N-(5-(3-(7-(Piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclopropanecarboxamide 151.

White solid (8.0 mg, 0.02 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 0.86-0.92 (m, 4H), 1.75 (brs, 6H), 1.86-1.95 (m, 1H), 4.28 (brs, 2H), 6.94 (d, J=7.15 Hz, 1H), 7.96 (brs, 1H), 8.67 (brs, 1H), 8.73 (s, 2H), 8.85 (d, J=2.01 Hz, 1H), 9.06 (d, J=1.51 Hz, 1H), 10.82 (s, 1H), 14.60 (brs, 1H); ESIMS found for C₂₆H₂₅N₉O m/z 480.1 (M+1).

5-(5-((3,3-Difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine 157.

White solid (31.0 mg, 0.06 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.76 (brs, 6H), 2.56-2.67 (m, 2H), 3.47-3.59 (m, 4H), 3.80-394 (m, 4H), 4.57 (brs, 2H), 6.96 (d, J=7.28 Hz, 1H), 7.98 (brs, 1H), 8.72 (brs, 1H), 8.88 (s, 1H), 8.93 (s, 1H), 9.11 (d, J=1.51 Hz, 1H), 9.14 (d, J=2.13 Hz, 1H), 14.70 (brs, 1H), 14.99 (brs, 1H); ESIMS found for C₂₇H₂₇F₂N₉ m/z 516.2 (M+1).

3-Methyl-N-(5-(3-(7-(4-methyl-1H-imidazol-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)butanamide 160.

White solid (6.2 mg, 0.01 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 0.95 (d, J=6.65 Hz, 6H), 2.13 (non, J=6.78 Hz, 1H), 2.39 (d, J=7.03 Hz, 2H), 2.45 (s, 3H), 7.83 (d, J=5.40 Hz, 1H), 8.54 (d, J=5.40 Hz, 1H), 8.63 (s, 1H), 9.05 (d, J=1.88 Hz, 1H), 9.11 (s, 1H), 9.19 (s, 1H), 9.22 (s, 1H), 9.37 (d, J=1.63 Hz, 1H), 10.24 (d, J=1.13 Hz, 1H), 11.73 (s, 1H); ESIMS found for C₂₆H₂₄N₁₀O m/z 493.2 (M+1).

N-(5-(3-(7-(4-Methyl-1H-imidazol-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)pivalamide 165.

White solid (6.2 mg, 0.01 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.30 (s, 9H), 2.42 (s, 3H), 7.81 (d, J=5.27 Hz, 1H), 8.58 (d, J=5.40 Hz, 1H), 8.62 (brs, 1H), 8.72 (brs, 1H), 8.89 (s, 1H), 9.03 (d, J=2.01 Hz, 1H), 9.07 (s, 1H), 9.14 (d, J=1.76 Hz, 1H), 9.88 (s, 1H), 10.12 (brs, 1H), 14.54 (brs, 1H), 14.74 (s, 1H); ESIMS found for C₂₆H₂₄N₁₀O m/z 493.2 (M+1).

3-(7-(4-Methyl-1H-imidazol-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(pyrrolidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine 171.

White solid (13.0 mg, 0.03 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.89-1.99 (m, 2H), 2.01-2.11 (m, 2H), 2.48 (s, 3H), 3.03-3.12 (m, 2H), 3.13-3.25 (m, 2H), 4.60 (brs, 2H), 7.83 (d, J=5.40 Hz, 1H), 8.61 (d, J=5.40 Hz, 1H), 8.65 (brs, 1H), 8.79-8.87 (m, 1H), 8.90 (brs, 1H), 9.18 (s, 1H), 9.28 (brs, 2H), 10.27 (brs, 1H), 11.55 (s, 1H), 14.77 (brs, 1H); ESIMS found for C₂₆H₂₄N₁₀ m/z 477.1 (M+1).

5-(3-(7-(4-Methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-amine 186.

White solid (100.4 mg, 0.24 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 2.38 (brs, 3H), 2.64-2.85 (m, 4H), 4.05 (brs, 4H), 5.54 (brs, 2H), 6.58 (d, J=6.15 Hz, 1H), 7.27 (s, 1H), 7.97-8.04 (m, 2H), 8.14 (s, 1H), 8.89 (s, 2H), 14.24 (brs, 1H); ESIMS found for C₂₂H₂₂N₁₀ m/z 427.1 (M+1).

N-(5-(3-(7-(4-Methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)isobutyramide 192.

White solid (14.0 mg, 0.03 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.18 (d, J=6.78 Hz, 6H), 2.79 (spt, J=6.76 Hz, 1H), 2.86 (brs, 3H), 3.29-3.42 (m, 2H), 3.70 (d, J=10.54 Hz, 2H), 3.84 (brs, 2H), 5.32 (brs, 2H), 7.04 (brs, 1H), 8.17 (brs, 1H), 8.80 (brs, 1H), 8.87 (brs, 1H), 8.89 (brs, 1H), 9.03 (d, J=1.88 Hz, 2H), 10.99 (brs, 1H), 11.52 (brs, 1H), 14.76 (d, J=2.13 Hz, 1H); ESIMS found for C₂₆H₂₈N₁₀O m/z 497.2 (M+1).

3-(7-(4-Methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(piperidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine 198.

White solid (10.0 mg, 0.02 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.35-1.47 (m, 1H), 1.68-1.76 (m, 1H), 1.76-1.92 (m, 4H), 2.83 (brs, 3H), 2.91-3.02 (m, 2H), 3.27-3.43 (m, 4H), 3.65 (d, J=12.05 Hz, 4H), 3.76-3.88 (m, 2H), 4.45 (d, J=3.51 Hz, 2H), 6.97 (brs, 1H), 8.15 (brs, 1H), 8.75 (s, 1H), 8.84 (s, 1H), 8.99 (d, J=1.76 Hz, 1H), 9.15 (d, J=2.13 Hz, 2H), 11.30 (brs, 1H), 11.58 (brs, 1H), 14.66 (brs, 1H); ESIMS found for C₂₈H₃₂N₁₀ m/z 509.2 (M+1).

N-(5-(3-(7-(4-Methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclopentanecarboxamide 204.

White solid (11.0 mg, 0.02 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.52-1.64 (m, 2H), 1.65-1.85 (m, 4H), 1.88-2.00 (m, 2H), 2.85 (s, 3H), 2.93-3.03 (m, 1H), 3.24-3.44 (m, 4H), 3.77-3.99 (m, 2H), 5.31 (brs, 2H), 7.05 (brs, 1H), 8.18 (brs, 1H), 8.80 (brs, 1H), 8.88 (d, J=1.76 Hz, 1H), 8.90 (brs, 1H), 9.02 (d, J=2.01 Hz, 1H), 9.07 (brs, 1H), 11.09 (brs, 1H), 11.53 (brs, 1H), 14.78 (brs, 1H); ESIMS found for C₂₈H₃₀N₁₀O m/z 523.3 (M+1).

3-(7-(4-Methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyrimidin-5-yl)-1H-pyrazolo[3,4-b]pyridine 209.

White solid (27.0 mg, 0.07 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 2.83 (d, J=1.00 Hz, 3H), 3.25-3.38 (m, 2H), 3.63 (d, J=11.54 Hz, 2H), 3.77-3.93 (m, 4H), 7.02-7.11 (m, 1H), 8.18 (brs, 1H), 8.96 (s, 1H), 9.11 (d, J=2.01 Hz, 1H), 9.29 (s, 1H), 9.31 (s, 2H), 11.60 (brs, 1H), 14.77 (brs, 1H); ESIMS found for C₂₁H₂₀N₁₀ m/z 413.1 (M+1).

3-(3H-Imidazo[4,5-b]pyridin-2-yl)-5-(pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine 212.

White solid (41.2 mg, 0.13 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 7.53 (dd, J=7.47, 5.21 Hz, 1H), 8.06 (dd, J=7.97, 5.46 Hz, 1H), 8.33 (d, J=7.91 Hz, 1H), 8.56 (d, J=4.27 Hz, 1H), 8.85 (d, J=8.03 Hz, 1H), 8.91 (d, J=5.65 Hz, 1H), 9.15 (q, J=2.09 Hz, 2H), 9.36 (d, J=1.38 Hz, 1H), 14.89 (brs, 1H); ESIMS found for C₁₇H₁₁N₇ m/z 314.0 (M+1).

N-(5-(3-(3H-Imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-2-phenylacetamide 216.

White solid (9.4 mg, 0.02 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 3.78 (s, 2H), 7.24-7.30 (m, 1H), 7.32-7.42 (m, 4H), 7.45 (dd, J=7.53, 5.02 Hz, 1H), 8.23 (d, J=8.66 Hz, 1H), 8.51 (d, J=4.52 Hz, 1H), 8.60 (s, 1H), 8.86 (s, 1H), 8.99 (s, 1H), 9.01 (d, J=2.13 Hz, 1H), 9.04 (d, J=2.13 Hz, 1H), 10.96 (s, 1H), 14.74 (brs, 1H); ESIMS found for C₂₅H₁₈N₈O m/z 447.2 (M+1).

N-(5-(3-(3H-Imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-3,3-dimethylbutanamide 222.

White solid (7.0 mg, 0.02 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.07 (s, 9H), 2.34 (s, 2H), 7.54 (dd, J=7.65, 5.27 Hz, 1H), 8.33 (d, J=7.65 Hz, 1H), 8.56 (d, J=4.89 Hz, 1H), 8.73 (s, 1H), 8.97 (s, 1H), 9.03-9.06 (m, 1H), 9.07 (d, J=2.13 Hz, 1H), 9.14 (s, 1H), 10.90 (brs, 1H), 14.89 (brs, 1H); ESIMS found for C₂₃H₂₂N₈O m/z 427.1 (M+1).

N-(5-(3-(3H-Imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl) pyridin-3-yl)cyclohexanecarboxamide 228.

White solid (9.0 mg, 0.02 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.14-1.37 (m, 3H), 1.45 (qd, J=12.17, 2.13 Hz, 2H), 1.67 (d, J=12.55 Hz, 1H), 1.74-1.82 (m, 2H), 1.89 (d, J=12.55 Hz, 2H), 2.41-2.49 (m, 1H), 7.55 (dd, J=7.97, 5.33 Hz, 1H), 8.34 (d, J=7.91 Hz, 1H), 8.56 (dd, J=5.27, 1.00 Hz, 1H), 8.76 (t, J=1.88 Hz, 1H), 8.97 (d, J=1.76 Hz, 1H), 9.02-9.06 (m, 1H), 9.06 (d, J=2.26 Hz, 1H), 9.11 (d, J=2.01 Hz, 1H), 10.86 (s, 1H), 14.90 (brs, 1H); ESIMS found for C₂₄H₂₂N₈O m/z 439.1 (M+1).

5-(4-Methylpyridin-3-yl)-3-(7-(thiophen-3-yl)-3H-imidazo[4,5-b] pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine 237.

White solid (20.0 mg, 0.05 mmol). ¹H NMR (CD₃OD, 400 MHz) δ ppm 2.71 (s, 3H), 7.82 (dd, J=5.14, 2.89 Hz, 1H), 7.93 (d, J=4.14 Hz, 1H), 7.98 (d, J=6.27 Hz, 1H), 8.18 (d, J=6.27 Hz, 1H), 8.58 (d, J=6.27 Hz, 1H), 8.65 (brs, 1H), 8.82 (d, J=2.13 Hz, 1H), 8.83-8.87 (m, 1H), 8.97 (s, 1H), 9.09 (d, J=2.26 Hz, 1H); ESIMS found for C₂₂H₁₅N₇S m/z 410.2 (M+1).

N-(5-(3-(7-(Thiophen-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)benzamide 243.

White solid (11.0 mg, 0.02 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 7.56-7.63 (m, 2H), 7.64-7.70 (m, 2H), 7.70-7.76 (m, 1H), 8.08 (d, J=7.40 Hz, 2H), 8.21 (brs, 1H), 8.38 (d, J=5.02 Hz, 1H), 8.84 (brs, 1H), 8.89 (brs, 1H), 8.93 (brs, 1H), 9.05 (brs, 1H), 9.10 (s, 1H), 9.18 (s, 1H), 10.73 (s, 1H), 13.98 (brs, 1H); ESIMS found for C₂₈H₁₈N₈OS m/z 515.1 (M+1).

N-(5-(3-(7-(Thiophen-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)butyramide 249.

White solid (13.0 mg, 0.03 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 0.97 (t, J=7.40 Hz, 3H), 1.70 (sxt, J=7.33 Hz, 2H), 2.45 (t, J=7.28 Hz, 3H), 7.70 (d, J=5.14 Hz, 1H), 7.79 (dd, J=5.02, 3.01 Hz, 1H), 8.20 (d, J=4.52 Hz, 1H), 8.40 (d, J=5.27 Hz, 1H), 8.82 (brs, 1H), 8.91 (d, J=1.63 Hz, 1H), 8.95 (s, 2H), 9.06 (d, J=2.01 Hz, 1H), 9.15 (d, J=2.26 Hz, 1H), 10.71 (brs, 1H), 14.62 (brs, 1H); ESIMS found for C₂₅H₂₀N₈OS m/z 481.1 (M+1).

N-((5-(3-(7-(Furan-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo [3,4-b]pyridin-5-yl)pyridin-3-yl)methyl)ethanamine 264.

White solid (20.0 mg, 0.05 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.31 (t, J=7.09 Hz, 3H), 3.07 (q, J=5.02 Hz, 2H), 4.47 (brs, 3H), 7.44 (s, 1H), 7.72 (d, J=5.40 Hz, 1H), 7.95 (s, 1H), 8.44 (d, J=5.27 Hz, 1H), 9.09 (s, 1H), 9.14 (s, 1H), 9.19 (s, 1H), 9.21 (s, 1H), 9.33 (s, 1H), 9.45 (s, 1H), 10.17 (brs, 2H), 14.91 (brs, 1H); ESIMS found for C₂₄H₂₀N₈O m/z 437.2 (M+1).

5-(3-(7-(Furan-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)-N-isopropylpyridin-3-amine 270.

White solid (20.0 mg, 0.05 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.22 (d, J=6.27 Hz, 6H), 3.81 (brs, 1H), 6.39 (brs, 1H), 7.43 (s, 1H), 7.52 (brs, 1H), 7.57 (d, J=5.14 Hz, 1H), 7.90 (t, J=1.63 Hz, 1H), 8.07 (s, 1H), 8.29 (brs, 1H), 8.35 (d, J=5.02 Hz, 1H), 8.97 (s, 1H), 8.99 (s, 1H), 9.08 (s, 1H), 13.93 (brs, 1H), 14.55 (brs, 1H); ESIMS found for C₂₄H₂₀N₈O m/z 437.2 (M+1).

N-(5-(3-(7-(Furan-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo [3,4-b]pyridin-5-yl)pyridin-3-yl)pentanamide 276.

White solid (20.0 mg, 0.04 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 0.92 (t, J=7.34 Hz, 3H), 1.37 (sxt, J=7.52 Hz, 2H), 1.64 (quin, J=7.37 Hz, 2H), 2.43-2.48 (m, 3H), 7.46 (d, J=1.13 Hz, 1H), 7.62 (d, J=5.02 Hz, 1H), 7.90 (s, 1H), 8.39 (d, J=5.27 Hz, 1H), 8.80 (s, 1H), 8.98 (s, 1H), 9.06 (brs, 3H), 9.04 (d, J=2.13 Hz, 1H), 9.14 (d, J=2.01 Hz, 1H), 10.88 (s, 1H), 14.67 (brs, 1H); ESIMS found for C₂₆H₂₂N₈O₂ m/z 479.2 (M+1).

1-Cyclopentyl-N-((5-(3-(7-(furan-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methyl)methanamine 282.

White solid (24.9 mg, 0.05 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.20-1.32 (m, 2H), 1.48-1.65 (m, 4H), 1.76-1.87 (m, 2H), 2.24 (spt, J=7.80 Hz, 1H), 2.95-3.04 (m, 2H), 4.34 (brs, 2H), 7.44 (brs, 1H), 7.57 (d, J=5.02 Hz, 1H), 7.90 (t, J=1.51 Hz, 1H), 8.36 (d, J=5.02 Hz, 1H), 8.58 (s, 1H), 8.80 (d, J=1.76 Hz, 1H), 9.01 (s, 1H), 9.09 (d, J=2.26 Hz, 1H), 9.16 (dd, J=5.02, 1.76 Hz, 4H), 13.95 (brs, 1H), 14.57 (s, 1H); ESIMS found for C₂₈H₂₆N₈O m/z 491.2 (M+1).

N-(5-(3-(7-(Thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)propionamide 285.

White solid (11.0 mg, 0.02 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.17 (t, J=7.47 Hz, 3H), 7.33 (t, J=4.0 Hz, 1H), 7.68 (d, J=5.02 Hz, 1H), 7.84 (d, J=4.77 Hz, 1H), 8.32-8.39 (m, 2H), 8.88 (brs, 1H), 8.89 (brs, 1H), 8.92 (s, 1H), 9.08 (d, J=2.01 Hz, 1H), 9.27 (d, J=1.88 Hz, 1H), 10.70 (brs, 1H), 14.60 (brs, 1H); ESIMS found for C₂₄H₁₈N₈OS m/z 467.1 (M+1).

N,N-Dimethyl-5-(3-(7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-amine 291.

White solid (12.0 mg, 0.03 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 3.20 (brs, 7H), 7.31 (brs, 1H), 7.68 (d, J=4.52 Hz, 1H), 7.85-7.90 (m, 1H), 8.12 (brs, 1H), 8.30 (brs, 1H), 8.37 (brs, 2H), 8.61 (brs, 1H), 9.18 (brs, 1H), 9.34 (brs, 1H), 14.06 (brs, 1H), 14.63 (brs, 1H); ESIMS found for C₂₃H₁₈N₈S m/z 439.1 (M+1).

N,N-Dimethyl-1-(5-(3-(7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methanamine 297.

White solid (12.8 mg, 0.03 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 2.82 (d, J=4.64 Hz, 6H), 4.51 (d, J=4.89 Hz, 3H), 7.29-7.35 (m, 1H), 7.68 (d, J=5.27 Hz, 1H), 7.87 (d, J=4.89 Hz, 1H), 8.34-8.41 (m, 2H), 8.75 (brs, 1H), 8.93 (brs, 1H), 9.15 (s, 1H), 9.25 (s, 1H), 9.30 (d, J=2.01 Hz, 1H), 11.14 (brs, 1H), 14.64 (brs, 1H); ESIMS found for C₂₄H₂₀N₈S m/z 453.2 (M+1).

N-(5-(3-(7-(Thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclopropanecarboxamide 303.

White solid (21.0 mg, 0.04 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 0.87-0.97 (m, 4H), 1.87-1.96 (m, 1H), 7.31 (dd, J=4.77, 3.89 Hz, 1H), 7.67 (d, J=5.14 Hz, 1H), 7.79 (d, J=4.77 Hz, 1H), 8.30-8.39 (m, 2H), 8.79 (s, 1H), 8.87 (d, J=4.89 Hz, 2H), 9.07 (d, J=2.13 Hz, 1H), 9.25 (d, J=1.88 Hz, 1H), 10.94 (s, 1H), 14.04 (brs, 1H), 14.57 (brs, 1H); ESIMS found for C₂₅H₁₈N₈OS m/z 479.2 (M+1).

5-(5-((3,3-Difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-3-(7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine 309.

White solid (20.0 mg, 0.04 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 2.66 (brs, 2H), 3.71 (brs, 2H), 4.01 (t, J=12.17 Hz, 2H), 4.79 (brs, 2H), 7.33 (t, J=4.08 Hz, 1H), 7.69 (d, J=5.14 Hz, 1H), 7.99 (d, J=4.64 Hz, 1H), 8.30-8.41 (m, 2H), 9.16 (brs, 1H), 9.21 (brs, 2H), 9.27 (s, 1H), 9.35 (brs, 1H), 14.75 (brs, 1H); ESIMS found for C₂₆H₂₀F₂N₈S m/z 515.1 (M+1).

N-(5-(3-(7-(5-Fluorothiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-3-methylbutanamide 312.

White solid (46.8 mg, 0.09 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 0.97 (d, J=6.53 Hz, 6H), 2.12 (non, J=6.4 Hz, 1H), 2.30 (d, J=7.15 Hz, 2H), 6.95 (d, J=3.89 Hz, 1H), 7.68 (d, J=5.27 Hz, 1H), 8.00 (t, J=3.64 Hz, 1H), 8.34 (d, J=5.27 Hz, 1H), 8.74 (s, 1H), 8.82 (s, 1H), 8.86 (s, 1H), 9.06 (s, 1H), 9.24 (s, 1H), 10.52 (brs, 1H), 14.06 (brs, 1H), 14.58 (s, 1H); ESIMS found for C₂₆H₂₁FN₈OS m/z 513.2 (M+1).

5-(3-(7-(5-Methylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-amine 339.

White solid (126.0 mg, 0.30 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 2.60 (s, 3H), 5.58 (s, 2H), 6.99 (d, J=2.89 Hz, 1H), 7.37 (t, J=2.24 Hz, 1H), 7.62 (d, J=5.15 Hz, 1H), 8.04 (d, J=2.51 Hz, 1H), 8.06 (d, J=3.64 Hz, 1H), 8.25 (d, J=2.01 Hz, 1H), 8.30 (d, J=5.15 Hz, 1H), 8.95 (d, J=2.13 Hz, 1H), 9.32 (d, J=2.26 Hz, 1H), 13.95 (brs, 1H), 14.42 (s, 1H); ESIMS found for C₂₂H₁₆N₈S m/z 425.0 (M+1).

N-(5-(3-(7-(5-Methylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)isobutyramide 345.

White solid (9.0 mg, 0.02 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.16 (d, J=6.90 Hz, 6H), 2.54 (s, 3H), 2.67-2.75 (m, 1H), 7.02 (d, J=2.64 Hz, 1H), 7.58 (d, J=5.40 Hz, 1H), 8.14 (d, J=3.76 Hz, 1H), 8.31 (d, J=5.15 Hz, 1H), 8.60 (t, J=2.32 Hz, 1H), 8.77 (d, J=1.88 Hz, 1H), 8.85 (d, J=2.51 Hz, 1H), 9.02 (d, J=2.26 Hz, 1H), 9.32 (d, J=1.88 Hz, 1H), 10.31 (s, 1H), 13.97 (s, 1H), 14.50 (s, 1H); ESIMS found for C₂₆H₂₂N₈OS m/z 495.1 (M+1).

3-(7-(5-Methylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(piperidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine 351.

White solid (10.8 mg, 0.02 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.33-1.46 (m, 1H), 1.66-1.74 (m, 1H), 1.76-1.86 (m, 4H), 2.61 (s, 3H), 2.88-3.01 (m, 2H), 3.42 (d, J=9.79 Hz, 2H), 4.47 (d, J=5.02 Hz, 2H), 7.02 (d, J=3.64 Hz, 1H), 7.62 (d, J=5.40 Hz, 1H), 8.15 (d, J=3.64 Hz, 1H), 8.33 (d, J=5.27 Hz, 1H), 8.75 (brs, 1H), 8.89 (s, 1H), 9.17 (d, J=2.26 Hz, 1H), 9.25 (d, J=2.01 Hz, 1H), 9.41 (d, J=2.13 Hz, 1H), 10.73 (brs, 1H), 14.58 (brs, 1H); ESIMS found for C₂₈H₂₆N₈S m/z 507.3 (M+1).

N-(5-(3-(7-(5-Methylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclopentanecarboxamide 357.

White solid (12.0 mg, 0.02 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.54-1.84 (m, 6H), 1.87-1.98 (m, 2H), 2.87-2.97 (m, 1H), 7.01 (d, J=1.38 Hz, 1H), 7.59 (d, J=4.77 Hz, 1H), 8.16 (d, J=2.89 Hz, 1H), 8.31 (d, J=4.89 Hz, 1H), 8.85 (brs, 1H), 8.99 (brs, 1H), 9.06 (brs, 1H), 9.12 (brs, 1H), 9.28 (brs, 1H), 10.95 (brs, 1H), 14.64 (brs, 1H); ESIMS found for C₂₈H₂₄N₈OS m/z 521.2 (M+1).

N-(5-(3-(7-(5-Acetylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclohexanecarboxamide 384.

White solid (17.0 mg, 0.03 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.19-1.36 (m, 4H), 1.38-1.51 (m, 2H), 1.66 (d, J=10.92 Hz, 1H), 1.77 (d, J=12.92 Hz, 2H), 1.88 (brs, 1H), 2.43-2.48 (m, 1H), 2.56 (s, 3H), 7.76 (d, J=5.27 Hz, 1H), 8.07 (d, J=4.02 Hz, 1H), 8.37-8.46 (m, 2H), 8.90 (s, 1H), 9.07 (d, J=2.13 Hz, 1H), 9.09 (s, 1H), 9.27 (d, J=2.01 Hz, 1H), 9.29 (d, J=1.51 Hz, 1H), 11.03 (s, 1H), 14.71 (brs, 1H); ESIMS found for C₃₀H₂₆N₈O₂S m/z 563.2 (M+1).

N-(3-Fluoro-5-(2-(5-(4-methylpyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)benzyl)methanesulfonamide 393.

White solid (17.0 mg, 0.03 mmol). ¹H NMR (CD₃OD, 400 MHz) δ ppm 2.68 (s, 3H), 2.89 (s, 3H), 4.37 (s, 2H), 7.36 (d, J=8.85 Hz, 1H), 7.72 (d, J=5.65 Hz, 1H), 7.85 (d, J=9.42 Hz, 1H), 8.08 (brs, 1H), 8.14 (d, J=6.22 Hz, 1H), 8.54 (d, J=5.46 Hz, 1H), 8.77 (d, J=2.07 Hz, 1H), 8.80 (d, J=6.03 Hz, 1H), 8.92 (s, 1H), 9.08 (d, J=2.07 Hz, 1H); ESIMS found for C₂₆H₂₁FN₈O₂S m/z 529.2 (M+1).

N-(3-Fluoro-5-(2-(5-(pyrimidin-5-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)benzyl)methanesulfonamide 414.

White solid (26.0 mg, 0.05 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 2.93 (s, 3H), 4.33 (d, J=6.15 Hz, 2H), 7.34 (d, J=9.41 Hz, 1H), 7.67 (d, J=5.02 Hz, 1H), 7.83 (t, J=6.27 Hz, 1H), 8.23 (brs, 1H), 8.45 (d, J=5.02 Hz, 1H), 8.78-8.99 (m, 3H), 9.11 (d, J=2.01 Hz, 1H), 9.18 (d, J=1.88 Hz, 1H), 9.27 (s, 1H), 9.32 (s, 2H), 14.12 (brs, 1H), 14.66 (s, 1H); ESIMS found for C₂₄H₁₈FN₉O₂S m/z 516.2 (M+1).

N¹-(3-Fluoro-5-(2-(5-(5-(isopropylamino)pyridin-3-yl)-1H-pyrazolo [3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)phenyl)-N²,N²-dimethylethane-1,2-diamine 426.

White solid (16.0 mg, 0.03 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.22 (d, J=6.02 Hz, 6H), 2.80 (brs, 6H), 3.23 (brs, 2H), 3.82-3.93 (m, 2H), 6.62 (d, J=11.17 Hz, 1H), 7.34 (brs, 1H), 7.52 (brs, 1H), 7.61 (d, J=4.77 Hz, 1H), 7.96 (brs, 1H), 8.16 (brs, 1H), 8.47 (brs, 1H), 8.50 (brs, 1H), 9.03 (brs, 1H), 9.09 (brs, 1H), 10.56 (brs, 1H), 14.73 (brs, 1H); ESIMS found for C₃₀H₃₁FN₁₀ m/z 551.2 (M+1).

N-(5-(3-(7-(3-((2-(Dimethylamino)ethyl)amino)-5-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)pentanamide 432.

White solid (29.6 mg, 0.05 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 0.92 (t, J=7.34 Hz, 3H), 1.36 (sxt, J=7.36 Hz, 2H), 1.62 (quin, J=7.47 Hz, 2H), 2.43 (t, J=7.40 Hz, 2H), 2.82 (d, J=3.76 Hz, 6H), 3.22-3.30 (m, 3H), 6.60 (d, J=11.17 Hz, 1H), 7.48 (brs, 2H), 7.59 (d, J=5.14 Hz, 1H), 8.45 (d, J=5.15 Hz, 1H), 8.55 (s, 1H), 8.87 (s, 1H), 8.99 (d, J=2.01 Hz, 2H), 9.11 (d, J=2.13 Hz, 1H), 10.06 (brs, 1H), 10.58 (brs, 1H), 14.61 (brs, 1H); ESIMS found for C₃₂H₃₃FN₁₀O m/z 593.3 (M+1).

3-(7-(3-Fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(piperidin-4-yl)-1H-pyrazolo[3,4-b]pyridine 909.

Gray solid (4.0 mg, 0.008 mmol). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 0.85 (br t, J=7.00 Hz, 2H), 1.48 (br s, 1H), 1.90-2.03 (m, 2H), 2.15-2.22 (m, 2H), 3.04-3.12 (m, 1H), 3.43 (br d, J=12.90 Hz, 1H), 7.35-7.43 (m, 1H), 7.71 (br s, 2H), 8.38 (br s, 1H), 8.44 (br d, J=4.67 Hz, 1H), 8.53 (br s, 1H), 8.64 (s, 1H), 8.73 (s, 1H); ESIMS found for C₂₃H₂₀FN₇ m/z 414.3 (M+1).

3-(7-(3-Fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(1,2,3,6-tetrahydropyridin-4-yl)-1H-pyrazolo[3,4-b]pyridine 910.

Yellow solid (7.0 mg, 0.01 mmol). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 2.60 (br s, 2H), 3.10 (br s, 2H), 3.53 (br s, 2H), 6.48 (br s, 1H), 7.39 (s, 1H), 7.61-7.67 (m, 1H), 7.69 (br s, 1H), 8.24 (br s, 1H), 8.43 (d, J=5.21 Hz, 1H), 8.65 (br s, 1H), 8.88 (s, 1H), 8.89 (s, 1H); ESIMS found for C₂₃H₁₈FN₇ m/z 412.2 (M+1).

3-(7-(3-Fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(1H-pyrazol-4-yl)-1H-pyrazolo[3,4-b]pyridine 911.

Black solid (5.6 mg, 0.01 mmol). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 7.36-7.44 (m, 1H), 7.60-7.75 (m, 3H), 8.27 (br d, J=7.96 Hz, 1H), 8.34 (br s, 1H), 8.43 (d, J=5.21 Hz, 1H), 8.70 (br d, J=11.25 Hz, 1H), 8.98 (d, J=1.65 Hz, 1H), 9.01 (d, J=1.92 Hz, 1H), 13.16 (br s, 1H), 13.98 (s, 1H), 14.31 (s, 1H); ESIMS found for C₂₁H₁₃FN₈ m/z 397.1 (M+1).

3-(7-(3-Fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(1-methyl-1H-pyrazol-4-yl)-1H-pyrazolo[3,4-b]pyridine 912.

Orange solid (21.0 mg, 0.05 mmol). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 3.94 (s, 3H), 7.40 (br t, J=7.55 Hz, 1H), 7.64-7.74 (m, 2H), 7.97 (s, 1H), 8.22-8.30 (m, 2H), 8.43 (d, J=5.21 Hz, 1H), 8.70 (br d, J=11.25 Hz, 1H), 8.95 (br s, 1H), 8.96 (br s, 1H), 13.98 (br s, 1H), 14.32 (br s, 1H); ESIMS found for C₂₂H₁₅FN₈ m/z 411.1 (M+1).

5-(1,2-Dimethyl-1H-imidazol-5-yl)-3-(7-(3-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine 913.

Yellow solid (1.3 mg, 0.003 mmol). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 2.42 (s, 3H), 3.66 (s, 3H), 7.09 (s, 1H), 7.33-7.41 (m, 1H), 7.60-7.71 (m, 2H), 8.25 (br d, J=7.68 Hz, 1H), 8.43 (br d, J=5.21 Hz, 1H), 8.49 (br d, J=10.15 Hz, 1H), 8.77 (s, 1H), 8.86 (s, 1H), 14.00 (brs, 1H), 14.46 (brs, 1H); ESIMS found for C₂₃H₁₇FN₈ m/z 425.1 (M+1).

1-(6-(3-(7-(3-Fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo [3,4-b]pyridin-5-yl)pyrazin-2-yl)azetidin-3-amine 914.

Yellow solid (42.3 mg, 0.09 mmol). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 3.69-3.78 (m, 2H), 3.92 (br s, 1H), 4.30 (br t, J=7.82 Hz, 2H), 7.30-7.39 (m, 1H), 7.51 (br d, J=4.67 Hz, 1H), 7.58-7.67 (m, 1H), 7.86 (s, 1H), 8.26-8.36 (m, 2H), 8.48 (br d, J=12.62 Hz, 1H), 8.52 (s, 1H), 9.21 (s, 1H), 9.40 (s, 1H); ESIMS found for C₂₅H₁₉FN₁₀ m/z 479.2 (M+H).

5-(5-(Cyclohexyloxy)pyridin-3-yl)-3-(7-(3-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine 915.

Brown solid (34 mg, 0.067 mmol, 20.87% yield). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 1.25-1.36 (m, 1H), 1.36-1.46 (m, 2H), 1.47-1.59 (m, 3H), 1.69-1.79 (m, 2H), 1.95-2.05 (m, 2H), 4.59-4.69 (m, 1H), 7.34 (td, J=8.37, 1.92 Hz, 1H), 7.57-7.66 (m, 1H), 7.70 (d, J=5.21 Hz, 1H), 7.83 (t, J=2.20 Hz, 1H), 8.31 (d, J=7.96 Hz, 1H), 8.37 (d, J=2.47 Hz, 1H), 8.44 (d, J=4.94 Hz, 1H), 8.55 (br d, J=10.98 Hz, 1H), 8.60 (d, J=1.65 Hz, 1H), 9.07 (d, J=1.92 Hz, 1H), 9.12 (d, J=1.92 Hz, 1H), 14.05 (br s, 1H), 14.50 (br s, 1H); ESIMS found for C₂₉H₂₄FN₇O m/z 506.2 (M+1).

3-(7-(3-Fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(piperidin-4-yloxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine 916.

Dark yellow solid (22 mg, 0.041 mmol, 64.8% yield). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 1.85-1.97 (m, 2H), 2.12-2.22 (m, 2H), 3.11 (ddd, J=12.49, 8.51, 3.43 Hz, 2H), 3.26-3.32 (m, 2H), 4.88-4.97 (m, 1H), 7.37 (td, J=8.44, 2.33 Hz, 1H), 7.59-7.67 (m, 1H), 7.70 (br d, J=4.12 Hz, 1H), 7.93 (t, J=2.20 Hz, 1H), 8.30 (br d, J=8.23 Hz, 1H), 8.42-8.49 (m, 2H), 8.59 (br d, J=9.33 Hz, 1H), 8.68 (d, J=1.65 Hz, 1H), 9.08 (d, J=2.20 Hz, 1H), 9.14 (s, 1H); ESIMS found for C₂₈H₂₃FN₈O m/z 507.2 (M+1).

N-(5-(3-(7-(3-Fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo [3,4-b]pyridin-5-yl)pyridin-3-yl)-2-(piperidin-4-yl)acetamide 917.

Brown solid (25.1 mg, 0.05 mmol). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 1.28-1.39 (m, 2H), 1.80 (br d, J=13.45 Hz, 2H), 2.37 (br d, J=7.14 Hz, 2H), 2.75 (br t, J=12.90 Hz, 2H), 3.16 (br d, J=13.72 Hz, 1H), 7.29-7.36 (m, 1H), 7.62-7.70 (m, 2H), 8.31 (br s, 1H), 8.43-8.50 (m, 2H), 8.53 (br s, 1H), 8.74 (d, J=1.92 Hz, 1H), 8.80 (s, 1H), 8.99 (d, J=1.92 Hz, 1H), 9.09 (d, J=2.20 Hz, 1H), 10.36 (s, 1H); ESIMS found for C₃₀H₂₆FN₉O m/z 548.1 (M+1).

3-(7-(3-Fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(2-(pyrrolidin-1-yl)ethoxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine 918.

Beige solid (6.5 mg, 0.012 mmol, 65.2% yield). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 1.70 (br t, J=3.29 Hz, 4H), 2.56 (br s, 4H), 2.88 (br t, J=5.63 Hz, 2H), 4.29 (t, J=5.76 Hz, 2H), 7.34 (td, J=8.51, 2.47 Hz, 1H), 7.57-7.65 (m, 1H), 7.69 (br d, J=4.67 Hz, 1H), 7.86 (t, J=2.06 Hz, 1H), 8.30 (br d, J=7.68 Hz, 1H), 8.38 (d, J=2.74 Hz, 1H), 8.44 (d, J=5.21 Hz, 1H), 8.55 (br d, J=11.25 Hz, 1H), 8.63 (d, J=1.65 Hz, 1H), 9.08 (d, J=2.20 Hz, 1H), 9.14 (s, 1H), 14.05 (br s, 1H), 14.50 (br s, 1H); ESIMS found for C₂₉H₂₅FN₈O m/z 521.2 (M+1).

2-((5-(3-(7-(3-Fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo [3,4-b]pyridin-5-yl)pyridin-3-yl)oxy)-N,N-dimethylethan-1-amine 919.

Yellow solid (4.5 mg, 0.009 mmol). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 2.25 (s, 6H), 2.71 (t, J=5.63 Hz, 2H), 4.27 (t, J=5.76 Hz, 2H), 7.34 (td, J=8.58, 2.06 Hz, 1H), 7.59-7.66 (m, 1H), 7.69 (br s, 1H), 7.83-7.87 (m, 1H), 8.30 (br s, 1H), 8.38 (d, J=2.74 Hz, 1H), 8.44 (d, J=5.21 Hz, 1H), 8.55 (br d, J=11.80 Hz, 1H), 8.64 (d, J=1.92 Hz, 1H), 9.08 (d, J=2.20 Hz, 1H), 9.14 (s, 1H), 14.02 (br s, 1H), 14.46 (br s, 1H); ESIMS found for C₂₇H₂₃FN₈O m/z 495.2 (M+1).

3-(7-(3-Fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-methoxypyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine 920.

Orange solid (2.0 mg, 0.005 mmol). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 3.97 (s, 3H), 7.35 (td, J=8.37, 2.20 Hz, 1H), 7.57-7.69 (m, 2H), 7.81-7.85 (m, 1H), 8.27 (br s, 1H), 8.39 (d, J=2.74 Hz, 1H), 8.42 (d, J=4.94 Hz, 1H), 8.55 (br s, 1H), 8.64 (d, J=1.65 Hz, 1H), 9.05 (d, J=2.20 Hz, 1H), 9.14 (d, J=2.20 Hz, 1H); ESIMS found for C₂₄H₁₆FN₇O m/z 438.2 (M+1).

5-(3-(7-(3-Fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b] pyridin-5-yl)pyridin-3-ol 921.

Yellow solid (8.2 mg, 0.02 mmol). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 7.36 (td, J=8.78, 2.47 Hz, 1H), 7.56 (t, J=2.20 Hz, 1H), 7.63 (td, J=7.96, 6.31 Hz, 1H), 7.70 (d, J=4.94 Hz, 1H), 8.24 (d, J=2.47 Hz, 1H), 8.33 (d, J=7.68 Hz, 1H), 8.44 (d, J=5.21 Hz, 1H), 8.49-8.56 (m, 2H), 8.99 (d, J=2.47 Hz, 1H), 9.09 (d, J=2.20 Hz, 1H), 10.16 (s, 1H), 14.04 (s, 1H), 14.48 (s, 1H); ESIMS found for C₂₃H₁₄FN₇O m/z 424.1 (M+1).

5-(5-(Benzyloxy)pyridin-3-yl)-3-(7-(3-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine 922.

Brown solid (20 mg, 0.035 mmol, 12.85% yield). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 5.32 (s, 2H), 7.21-7.30 (m, 1H), 7.35-7.41 (m, 1H), 7.45 (t, J=7.55 Hz, 2H), 7.54 (d, J=7.14 Hz, 2H), 7.58-7.65 (m, 1H), 7.70 (d, J=4.94 Hz, 1H), 7.96 (s, 1H), 8.31 (br d, J=7.68 Hz, 1H), 8.44 (d, J=5.21 Hz, 1H), 8.46 (d, J=2.74 Hz, 1H), 8.56 (br d, J=11.25 Hz, 1H), 8.68 (d, J=1.37 Hz, 1H), 9.09 (d, J=1.92 Hz, 1H), 9.17 (d, J=1.92 Hz, 1H), 14.06 (br s, 1H), 14.51 (br s, 1H); ESIMS found for C₃₀H₂₀FN₇O m/z 514.2 (M+1).

2-Cyclohexyl-N-(5-(3-(7-(3-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide 923.

Amber colored solid (22 mg, 0.038 mmol, 20.97% yield). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 0.96-1.08 (m, 2H), 1.11-1.31 (m, 3H), 1.63 (br d, J=12.08 Hz, 1H), 1.68 (br d, J=12.90 Hz, 2H), 1.75 (br d, J=12.35 Hz, 2H), 1.82 (ddd, J=10.63, 7.07, 3.70 Hz, 1H), 2.29 (d, J=7.14 Hz, 2H), 7.29 (br t, J=7.55 Hz, 1H), 7.61-7.68 (m, 1H), 7.70 (br s, 1H), 8.36 (br s, 1 H), 8.44 (d, J=5.21 Hz, 1H), 8.49 (br d, J=9.33 Hz, 1H), 8.55 (br s, 1H), 8.72 (d, J=1.92 Hz, 1H), 8.79 (s, 1H), 8.99 (d, J=2.20 Hz, 1H), 9.08 (d, J=1.92 Hz, 1H), 10.27 (s, 1H), 14.07 (br s, 1H), 14.51 (br s, 1H); ESIMS found for C₃₁H₂₇FN₈O m/z 547.3 (M+1).

3-(7-(3-Fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyridin-4-yl)-1H-pyrazolo[3,4-b]pyridine 924.

Brown solid (25.0 mg, 0.06 mmol). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 7.39 (td, J=8.37, 2.20 Hz, 1H), 7.62-7.69 (m, 1H), 7.72 (d, J=5.21 Hz, 1H), 7.91 (d, J=6.04 Hz, 2H), 8.27 (d, J=7.96 Hz, 1H), 8.45 (d, J=5.21 Hz, 1H), 8.66 (br d, J=11.53 Hz, 1H), 8.74 (d, J=6.04 Hz, 2H), 9.15 (d, J=2.20 Hz, 1H), 9.28 (d, J=2.20 Hz, 1H), 14.08 (br s, 1H), 14.53 (s, 1H); ESIMS found for C₂₃H₁₄FN₇ m/z 408.1 (M+1).

3-(7-(3-Fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine 925.

Dark brown solid (15 mg, 0.033 mmol, 10.86% yield). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 7.35-7.43 (m, 1H), 7.46 (br t, J=6.04 Hz, 1H), 7.64-7.76 (m, 2H), 7.94-8.04 (m, 1H), 8.16 (br d, J=9.06 Hz, 1H), 8.32 (br d, J=9.88 Hz, 1H), 8.45 (d, J=7.14 Hz, 1H), 8.63 (br d, J=11.25 Hz, 1H), 8.78 (br d, J=4.12 Hz, 1H), 9.40 (s, 1H), 9.62 (s, 1H), 14.07 (br s, 1H), 14.47 (br s, 1H); ESIMS found for C₂₃H₁₄FN₇ m/z 408.1 (M+1).

3-(7-(3-Fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyrazin-2-yl)-1H-pyrazolo[3,4-b]pyridine 926.

Brown solid (10 mg, 0.023 mmol, 7.16% yield). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 7.40 (br t, J=8.23 Hz, 1H), 7.62-7.70 (m, 1H), 7.71 (br d, J=4.67 Hz, 1H), 8.32 (br d, J=7.41 Hz, 1H), 8.45 (br d, J=4.94 Hz, 1H), 8.60 (br d, J=10.98 Hz, 1H), 8.71 (d, J=2.20 Hz, 1H), 8.82 (s, 1H), 9.45 (d, J=2.20 Hz, 2H), 9.66 (s, 1H), 14.09 (br s, 1H), 14.56 (br s, 1H); ESIMS found for C₂₂H₁₃FN₈ m/z 409.1 (M+1).

N-(5-(3-(7-(3-(2-(Dimethylamino)ethoxy)-5-fluorophenyl)-3H-imidazo[4,5-b] pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-3-methylbutanamide 1234.

Orange solid (8.8 mg, 0.015 mmol, 10.6% yield). ¹H NMR (DMSO-d₆, 500 MHz) δ 0.97 (d, J=6.59 Hz, 6H), 2.04-2.17 (m, 7H), 2.27 (d, J=7.14 Hz, 2H), 2.43 (br s, 2H), 4.10 (br t, J=5.49 Hz, 2H), 6.93 (br d, J=10.43 Hz, 1H), 7.71 (br d, J=4.94 Hz, 1H), 7.96 (br d, J=10.98 Hz, 1H), 8.03 (br s, 1H), 8.39-8.47 (m, 2H), 8.69 (s, 1H), 8.86 (d, J=2.20 Hz, 1H), 8.96 (s, 1H), 9.04 (d, J=2.20 Hz, 1H), 10.26 (s, 1H), 14.06 (br s, 1H), 14.52 (br s, 1H); ESIMS found for C₃₂H₃₂FN₉O₂ m/z 594.3 (M+1).

N-(5-(3-(7-(3-Fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-3-methylbutanamide 1278.

Yellow solid (5.4 mg, 8.71 μmol, 13.63% yield). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 0.97 (d, J=6.59 Hz, 6H), 1.59 (br s, 4H), 2.12 (td, J=13.45, 6.59 Hz, 1H), 2.27 (br d, J=7.14 Hz, 2H), 2.38 (br s, 4H), 2.57 (br s, 2H), 4.12 (br t, J=5.76 Hz, 2H), 6.92 (br d, J=10.15 Hz, 1H), 7.69 (br s, 1H), 7.95 (br s, 1H), 8.06 (br s, 1H), 8.41 (br d, J=4.94 Hz, 1H), 8.43 (br s, 1H), 8.68 (s, 1H), 8.86 (d, J=1.92 Hz, 1H), 8.95 (s, 1H), 9.04 (s, 1H), 10.26 (s, 1H), 14.02 (brs, 1H), 14.42 (brs, 1H); ESIMS found for C₃₄H₃₄FN₉O₂ m/z 620.3 (M+1).

N-(5-(3-(7-(3-Fluoro-5-hydroxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-3-methylbutanamide 1322.

Yellow solid (5.4 mg, 10.33 μmol, 9.22% yield). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 0.98 (d, J=6.86 Hz, 6H), 2.08-2.18 (m, 1H), 2.28 (d, J=7.14 Hz, 2H), 6.67 (br d, J=10.43 Hz, 1H), 7.59 (br s, 1H), 7.75 (br s, 1H), 7.88 (br s, 1H), 8.39-8.45 (m, 2H), 8.74 (d, J=1.92 Hz, 1H), 8.86 (d, J=2.20 Hz, 1H), 8.98 (d, J=1.92 Hz, 1H), 9.11 (s, 1H), 10.12 (s, 1H), 10.22 (br s, 1H), 14.03 (br s, 1H), 14.52 (s, 1H); ESIMS found for C₂₈H₂₃FN₈O₂ m/z 523.2 (M+1).

N-(5-(3-(7-(3-Fluoro-5-methoxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-3-methylbutanamide 1366.

Yellow solid (19.8 mg, 0.037 mmol, 31.8% yield). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 0.97 (br d, J=6.59 Hz, 6H), 2.11 (dt, J=13.38, 6.90 Hz, 1H), 2.28 (br d, J=7.14 Hz, 2H), 3.83 (s, 3H), 6.92 (br d, J=10.70 Hz, 1H), 7.68 (br s, 1H), 7.92 (br s, 1H), 8.11 (br s, 1H), 8.41 (br d, J=4.94 Hz, 1H), 8.44 (br s, 1H), 8.70 (s, 1H), 8.85 (s, 1H), 8.94 (s, 1H), 9.07 (s, 1H), 10.26 (s, 1H); ESIMS found for C₂₉H₂₅FN₈O₂ m/z 537.2 (M+1).

2-(Dimethylamino)-N-(5-(3-(7-(3-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide 1409.

Brown solid (5.3 mg, 0.01 mmol). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 2.34 (s, 6H), 3.18 (s, 2H), 7.28-7.35 (m, 1H), 7.62-7.67 (m, 1H), 7.70 (br s, 1H), 8.34 (br s, 1H), 8.44 (br d, J=4.94 Hz, 1H), 8.50 (br s, 1H), 8.59 (br s, 1H), 8.75 (d, J=2.20 Hz, 1H), 8.95 (s, 1H), 9.01 (d, J=2.20 Hz, 1H), 9.11 (s, 1H), 10.12 (br s, 1H), 14.04 (brs, 1H), 14.49 (brs, 1H); ESIMS found for C₂₇H₂₂FN₉O m/z 508.1 (M+1).

Example 2

The screening assay for Wnt activity is described as follows. Reporter cell lines can be generated by stably transducing cancer cell lines (e.g., colon cancer) or primary cells (e.g., IEC-6 intestinal cells) with a lentiviral construct that includes a Wnt-responsive promoter driving expression of the firefly luciferase gene.

SW480 colon carcinoma cells were transduced with a lentiviral vector expressing luciferase with a human Sp5 promoter consisting of a sequence of eight TCF/LEF binding sites. SW480 cells stably expressing the Sp5-Luc reporter gene and a hygromycin resistance gene were selected by treatment with 150 μg/ml of hygromycin for 7 days. These stably transduced SW480 cells were expanded in cell culture and used for all further screening activities. For Sp5-Luc reporter gene assays, the cells were plated at 10,000 cells/well in 96-well plates with growth medium containing 10% fetal calf serum and incubated overnight at 37° C. and 5% CO₂. Each compound was dissolved in DMSO as a 10 mM stock in standard j-vials and used to prepare compound source plates in dose-response format with 3-fold serial dilutions and a 10 mM top concentration. Compound transfer from serially diluted source plates to assay plates containing the cells was accomplished using a pintool (Multimek 96, Beckman equipped with V&P Scientific FP1S50H pins) based liquid handling protocol. This protocol used a slotted pin to transfer 50 nl of compound from a source plate well to an assay plate well containing 50 μl of cells in growth medium. The 1000-fold dilution resulted in a final DMSO concentration of 0.1% on the cells in each well. Control wells received 50 nl of DMSO treatment for normalization and calculating IC₅₀ values. The treated cells were incubated at 37° C. and 5% CO₂ for an additional forty-two hours. Following incubation, the growth medium was removed and 50 μl of BrightGlo luminescence reagent (Promega) was added to each well of the 96-well assay plates. The plates were placed on an orbital shaker for 5 min and then luminescence was quantified on the Victor3 (Perkin Elmer) plate reader. Readings were normalized to DMSO only treated cells, and normalized activities were utilized for IC₅₀ calculations using the dose-response log (inhibitor) vs. response -variable slope (four parameters) nonlinear regression feature available in GraphPad Prism 5.0 or 6.0. Table 2 shows the measured activity for selected compounds of Formula I as described herein.

TABLE 2
Compound IC50 (μM)
5        0.1723
10       >10
21       0.0358
28       0.0423
33       0.1438
38       0.0115
44       0.3773
45       0.0037
57       0.1181
61       0.0420
68       >10
75       0.0454
78       >10
91       >10
102      2.3830
106      >10
114      >10
130      0.6328
133      0.0366
139      0.3845
145      2.7970
151      0.2470
157      4.2110
160      0.0083
165      >10
171      0.2762
186      >10
192      >10
198      >10
204      0.3125
209      >10
212      0.2145
216      0.7509
222      0.1919
228      >10
237      0.0216
243      0.0330
249      0.0027
264      >10
270      0.0443
276      0.0081
282      >10
285      0.0033
291      0.0438
297      >10
303      0.0077
309      0.0380
312      0.0270
339      0.0026
351      >10
357      0.0080
384      >10
393      0.0206
414      >10
426      0.1288
432      0.0217
909      >10
910      >10
911      0.030
912      0.221
913      0.066
914      1.023
915      >10
916      >10
917      >10
918      >10
919      >10
920      1.050
921      0.027
922      >10
923      >10
924      >10
925      >10
926      >10
1234     1.130
1278     0.890
1322     0.395
1366     0.125
1409     3.990

Example 3

The above synthesized compounds were screened using primary human mesenchymal stem cells (hMSCs) to determine their ability to induce chondrogenesis (process by which cartilage is developed).

Human Mesenchymal Stem Cell Culture:

Primary human mesenchymal stem cells (hMSCs) were purchased from Lonza (Walkersville, Md.) and expanded in Mesenchymal Stem Cell Growth Media (Lonza). Cells between passage 3 and 6 were used for the experiments.

Compound Screening:

Each compound was dissolved in DMSO as a 10 mM stock and used to prepare compound source plates. Serial dilution (1:3, 6-point dose-response curves from 2700 nM to 10 nM) and compound transfer was performed using the ECHO 550 (Labcyte, Sunnyvale, Calif.) into 96-well clear bottom assay plates (Greiner Bio-One) with appropriate DMSO backfill for a final DMSO concentration of 0.03%. hMSCs were plated at 20,000 cells/well in 250 μL/well Incomplete Chondrogenic Induction Medium (Lonza; DMEM, dexamethasone, ascorbate, insulin-transferrin-selenium [ITS supplement], gentamycin-amphotericin [GA-1000], sodium pyruvate, proline and L-glutamine). TGF-β3 (10 ng/mL) was used as a positive control for differentiation while negative control wells were treated with 75 nL DMSO for normalization and calculating EC₅₀ values. Cells were incubated at 37° C. and 5% CO₂ for 6 days. To image chondrogenic nodules, the cells were fixed using 4% formaldehyde (Electron Microscopy Sciences), and stained with 2 μg/mL Rhodamine B (Sigma-Aldrich) and 20 μM Nile Red (Sigma-Aldrich) [Johnson K., et. al, A Stem Cell-Based Approach to Cartilage Repair, Science, (2012), 336(6082). 717-721]. The nodules imaged (4 images per well at 4× magnification) by excitation at 531 nm and emission at 625 nm and quantified using the CellInsight CX5 (Thermo Scientific). Number of nodules in each well was normalized to the average of 3 DMSO treated wells on the same plate using Excel (Microsoft Inc.). The normalized averages (fold change over DMSO) of 3 replicate wells for each compound concentration were calculated. Due to solubility limitations of some of the compounds, curve fitting was incomplete leading to inaccurate EC₅₀ determinations.

Using TGF-β3 as a positive control, the concentration of test compounds required to induce equivalent levels of chondrogenesis is reported. In addition, the maximum activity of each compound and the respective dose that each compound reached maximum chondrogenesis activity is reported. Table 3 shows the activity of selected compounds as provided herein.

TABLE 3
			Max.	Conc
		Conc	Activity	(nM)
		(nM)	as %	of 100%
		of Max.	TGF-β3	TGF-β3
	Compound	activity	activity	activity
	38	300	N/A	48.6
	45	300	300	59.9
	75	30	N/A	58.5
	133	2700	300	316.9
	160	2700	N/A	26.8
	249	300	N/A	31.9
	285	2700	N/A	48.9
	339	300	N/A	43.2
	393	100	100	140.1
	432	300	N/A	57.0
	911	300	100	110.4
	913	900	300	117.0

Example 4

The above synthesized compounds were screened using primary human fibroblasts (derived from IPF patients) treated with TGF-β1 to determine their ability to inhibit the fibrotic process.

Human Fibroblast Cell Culture:

Primary human fibroblasts derived from IPF patients (LL29 cells) [¹Xiaoqiu Liu, et. al., “Fibrotic Lung Fibroblasts Show Blunted Inhibition by cAMP Due to Deficient cAMP Response Element-Binding Protein Phosphorylation”, Journal of Pharmacology and Experimental Therapeutics (2005), 315(2), 678-687; ²Watts, K. L., et. al., “RhoA signaling modulates cyclin D1 expression in human lung fibroblasts; implications for idiopathic pulmonary fibrosis”, Respiratory Research (2006), 7(1), 88] were obtained from American Type Culture Collection (ATCC) and expanded in F12 medium supplemented with 15% Fetal Bovine Serum and Penicillin/Streptomycin.

Compound Screening:

Each compound was dissolved in DMSO as a 10 mM stock and used to prepare compound source plates. Serial dilution (1:2, 11-point dose-response curves from 10 μM to 1.87 nM) and compound transfer was performed using the ECHO 550 (Labcyte, Sunnyvale, Calif.) into 384-well clear bottom assay plates (Greiner Bio-One) with appropriate DMSO backfill for a final DMSO concentration of 0.1%. LL29 cells were plated at 1,500 cells/well in 80 μl/well F12 medium supplemented with 1% Fetal Bovine Serum. One hour after addition of the cells, TGF-β1 (Peprotech; 20 ng/mL) was added to the plates to induce fibrosis (ref. 1 and 2 above). Wells treated with TGF-β1 and containing DMSO were used as controls. Cells were incubated at 37° C. and 5% CO₂ for 4 days. Following incubation for 4 days, SYTOX green nucleic acid stain (Life Technologies [Thermo Fisher Scientific]) was added to the wells at a final concentration of 1 uM and incubated at room temperature for 30 min. Cells were then fixed using 4% formaldehyde (Electron Microscopy Sciences), washed 3 times with PBS followed by blocking and permeabilization using 3% Bovine Serum Albumin (BSA; Sigma) and 0.3% Triton X-100 (Sigma) in PBS. Cells were then stained with antibody specific to α-smooth muscle actin (αSMA; Abcam) (ref. 1 and 2 above) in 3% Bovine Serum Albumin (BSA; Sigma) and 0.3% Triton X-100 (Sigma) in PBS, and incubated overnight at 4° C. Cells were then washed 3 times with PBS, followed by incubation with Alexa Flor-647 conjugated secondary antibody (Life Technologies [Thermo Fisher Scientific]) and DAPI at room temperature for 1 hour. Cells were then washed 3 times with PBS and plates were sealed for imaging. αSMA staining was imaged by excitation at 630 nm and emission at 665 nm and quantified using the Compartmental Analysis program on the CellInsight CX5 (Thermo Scientific). Dead or apoptotic cells were excluded from analysis based on positive SYTOX green staining. % of total cells positive for αSMA were counted in each well and normalized to the average of 11 wells treated with TGF-β1 on the same plate using Dotmatics' Studies Software. The normalized averages (fold change over untreated) of 3 replicate wells for each compound concentration were used to create dose-responses curves and EC₅₀ values were calculated using non-linear regression curve fit in the Dotmatics' Studies Software. The EC₅₀ values are reported.

Table 4 shows the activity of selected compounds as provided herein.

TABLE 4
         Inhibition of
         fibrosis
Compound EC50 (nM)
10       2.550
28       0.026
33       7.802
38       0.118
45       0.009
61       0.587
68       9.990
78       9.990
91       9.990
102      9.990
106      9.990
114      9.990
130      0.009
133      0.853
139      9.990
145      1.442
151      0.414
157      0.009
165      9.990
171      0.009
186      9.990
192      0.009
198      3.036
212      0.501
216      9.990
228      0.009
237      0.282
243      0.113
249      0.035
264      0.009
270      0.786
282      9.990
285      0.009
297      9.990
303      9.990
312      0.189
339      0.040
351      9.990
357      1.390
384      9.990
393      0.674
414      9.990
426      0.287
909      9.990
910      9.990
911      0.029
912      0.118
913      0.216
916      0.009
919      0.009
920      0.009
921      0.164
924      9.990
925      9.990
926      0.013
1409     0.009

Claims

1. A method of treating a disorder selected from the group consisting of: skin fibrosis; scleroderma; progressive systemic fibrosis; muscle fibrosis; glomerulosclerosis; glomerulonephritis; hypertrophic scar formation; uterine fibrosis; renal fibrosis; cirrhosis of the liver; liver fibrosis; adhesions occurring in the abdomen, pelvis, spine or tendons; chronic obstructive pulmonary disease; fibrosis following myocardial infarction; pulmonary fibrosis; idiopathic pulmonary fibrosis (IPF); fibrosis and scarring associated with diffuse/interstitial lung disease; central nervous system fibrosis; fibrosis following stroke; fibrosis associated with neuro-degenerative disorders selected from the group consisting of Alzheimer's Disease and multiple sclerosis, fibrosis associated with proliferative vitreoretinopathy (PVR); restenosis; endometriosis; ischemic disease; and radiation fibrosis in a subject, the method comprising administering to the subject a therapeutically effective amount of a compound of Formula I, or a pharmaceutically acceptable salt thereof:

2. The method of claim 1, wherein R1 is selected from the group consisting of -pyridinyl(R4)q, -pyrazinyl(R4)q, -pyrazolyl(R4)q, and -imidazolyl(R4)q.

3. The method of claim 2, wherein R1 is pyridinyl(R4)q.

4. The method of claim 3, wherein R4 is selected from the group consisting of CH2heterocyclyl(R9)h, —NHC(═O)R12, —NR13R14, —CH2NR15R16, and —OR22.

5. The method of claim 4, wherein R3, is -phenyl(R8)k.

6. The method of claim 5, wherein each R8 is independently selected from the group consisting of halide and —CH2NHSO2R17.

7. The method of claim 4, wherein R3 is selected from the group consisting of -pyridinyl(R6)q, -imidazolyl(R6)q, -furanyl(R6)q, and -thiophenyl(R6)q.

8. The method of claim 7, wherein q is 1 and R6 is selected from the group consisting of halide, —(C1-3 alkyl), and —C(═O)R17.

9. The method of claim 4, wherein R3 is selected from the group consisting of -piperidinyl(R7)h, -morpholinyl(R7)h, and -piperazinyl(R7)h.

10. The method of claim 9, wherein each R7 is independently selected from the group consisting of a halide and —(C1-3 alkyl).

11. A method of treating a disorder selected from the group consisting of: skin fibrosis; scleroderma; progressive systemic fibrosis; muscle fibrosis; glomerulosclerosis; glomerulonephritis; hypertrophic scar formation; uterine fibrosis; renal fibrosis; cirrhosis of the liver; liver fibrosis; adhesions occurring in the abdomen, pelvis, spine or tendons; chronic obstructive pulmonary disease; fibrosis following myocardial infarction; pulmonary fibrosis; idiopathic pulmonary fibrosis (IPF); fibrosis and scarring associated with diffuse/interstitial lung disease; central nervous system fibrosis; fibrosis following stroke; fibrosis associated with neuro-degenerative disorders selected from the group consisting of Alzheimer's Disease and multiple sclerosis, fibrosis associated with proliferative vitreoretinopathy (PVR); restenosis; endometriosis; ischemic disease; and radiation fibrosis in a subject, the method comprising administering to the subject a therapeutically effective amount of a compound of Formula I, or a pharmaceutically acceptable salt thereof:

12. The method of claim 11, wherein R1 is selected from the group consisting of -pyridinyl(R4)q, -pyrimidinyl(R4)q, -pyrazolyl(R4)q, and -imidazolyl(R4)q.

13. The method of claim 12, wherein R4 is selected from the group consisting of —(C1-3 alkyl), —CH2heterocyclyl(R9)h, —NHC(═O)R12, —NR13R14, —CH2NR15R16, and —OR22.

14. The method of claim 13, wherein R3 is -phenyl(R8)k.

15. The method of claim 13, wherein R3 is selected from the group consisting of -furanyl(R6)q and -thiophenyl(R6)q.

16. The method of claim 15, wherein q is 1 and R6 is selected from the group consisting of halide, —(C1-3 alkyl), and —C(═O)R17.

17. The method of claim 13, wherein R3 is selected from the group consisting of -piperidinyl(R7)h, -morpholinyl(R7)h, and -piperazinyl(R7)h.

18. The method of claim 17, wherein each R7 is independently selected from the group consisting of a halide and —(C1-3 alkyl).

19. The method of claim 1, wherein the compound of Formula I is selected from the group consisting of: 5-(3-(7-(3-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-amine [1];3-(7-(3-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [2];5-(3-(7-(3-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)-N,N-dimethylpyridin-3-amine [5];N-(5-(3-(7-(3-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-2-phenylacetamide [7];N-(5-(3-(7-(3-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)benzamide [8];5-(3-(7-(3-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)-N-isopropylpyridin-3-amine [9];1-(5-(3-(7-(3-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-N,N-dimethylmethanamine [10];3-(7-(3-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(pyrrolidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [11];3-(7-(3-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(piperidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [12];N-benzyl-1-(5-(3-(7-(3-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methanamine [19];1 cyclopentyl-N-((5-(3-(7-(3-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methyl)methanamine [20];5-(5-((3,3-difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-3-(7-(3-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [21];5-(3-(7-(4-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-amine [23];3-(7-(4-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [24];5-(3-(7-(4-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)-N,N-dimethylpyridin-3-amine [27];N-(5-(3-(7-(4-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-2-phenylacetamide [29];N-(5-(3-(7-(4-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)benzamide [30];5-(3-(7-(4-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)-N-isopropylpyridin-3-amine [31];1-(5-(3-(7-(4-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-N,N-dimethylmethanamine [32];3-(7-(4-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(pyrrolidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [33];3-(7-(4-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(piperidin-1l-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [34];N-benzyl-1-(5-(3-(7-(4-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methanamine [41];1-cyclopentyl-N-((5-(3-(7-(4-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methyl)methanamine [42];5-(5-((3,3-difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-3-(7-(4-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [43];5-(3-(7-(2-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-amine [45];3-(7-(2-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [46];5-(3-(7-(2-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)-N,N-dimethylpyridin-3-amine [49];N-(5-(3-(7-(2-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-2-phenylacetamide [51];N-(5-(3-(7-(2-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)benzamide [52];5-(3-(7-(2-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)-N-isopropylpyridin-3-amine [53];1-(5-(3-(7-(2-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-N,N-dimethylmethanamine [54];3-(7-(2-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(pyrrolidin-1-ylmethyl) pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [55];3-(7-(2-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(piperidin-1-ylmethyl) pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [56];N-benzyl-1-(5-(3-(7-(2-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methanamine [63];1-cyclopentyl-N-((5-(3-(7-(2-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methyl)methanamine [64];5-(5-((3,3-difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-3-(7-(2-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [65];5-(3-(7-(pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-amine [67];5-(pyridin-3-yl)-3-(7-(pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [68];N,N-dimethyl-5-(3-(7-(pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-amine [71];2-phenyl-N-(5-(3-(7-(pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [73];N-(5-(3-(7-(pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)benzamide [74];N-isopropyl-5-(3-(7-(pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-amine [75];N,N-dimethyl-1-(5-(3-(7-(pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methanamine [76];3-(7-(pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(pyrrolidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [77];5-(5-(piperidin--ylmethyl)pyridin-3-yl)-3-(7-(pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [78];N-benzyl-1-(5-(3-(7-(pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methanamine [85];1-cyclopentyl-N-((5-(3-(7-(pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methyl)methanamine [86];5-(5-((3,3-difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-3-(7-(pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [87];5-(3-(7-(pyridin-4-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-amine [89];5-(pyridin-3-yl)-3-(7-(pyridin-4-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [90];N,N-dimethyl-5-(3-(7-(pyridin-4-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-amine [93];2-phenyl-N-(5-(3-(7-(pyridin-4-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [95];N-(5-(3-(7-(pyridin-4-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)benzamide [96];N-isopropyl-5-(3-(7-(pyridin-4-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-amine [97];N,N-dimethyl-1-(5-(3-(7-(pyridin-4-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methanamine [98];3-(7-(pyridin-4-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(pyrrolidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [99];5-(5-(piperidin-1-ylmethyl)pyridin-3-yl)-3-(7-(pyridin-4-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [100];N-benzyl-1-(5-(3-(7-(pyridin-4-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methanamine [107];1-cyclopentyl-N-((5-(3-(7-(pyridin-4-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methyl)methanamine [108];5-(5-((3,3-difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-3-(7-(pyridin-4-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [109];5-(3-(7-(pyridin-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-amine [111];3-(7-(pyridin-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [112];N,N-dimethyl-5-(3-(7-(pyridin-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-amine [115];2-phenyl-N-(5-(3-(7-(pyridin-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [117];N-(5-(3-(7-(pyridin-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)benzamide [118];N-isopropyl-5-(3-(7-(pyridin-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-amine [119];N,N-dimethyl-1-(5-(3-(7-(pyridin-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methanamine [120];3-(7-(pyridin-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(pyrrolidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [121];5-(5-(piperidin-1-ylmethyl)pyridin-3-yl)-3-(7-(pyridin-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [122];N-benzyl-1-(5-(3-(7-(pyridin-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methanamine [129];1-cyclopentyl-N-((5-(3-(7-(pyridin-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methyl)methanamine [130];5-(5-((3,3-difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-3-(7-(pyridin-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [131];5-(3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-amine [135];3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [136];N,N-dimethyl-5-(3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-amine [139];2-phenyl-N-(5-(3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [142];N-(5-(3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)benzamide [143];N-isopropyl-5-(3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-amine [144];N,N-dimethyl-1-(5-(3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methanamine [145];3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(pyrrolidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [146];3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(piperidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [147];N-benzyl-1-(5-(3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methanamine [155];1-cyclopentyl-N-((5-(3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methyl)methanamine [156];5-(5-((3,3-difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [157];5-(3-(7-(4-methyl-1H-imidazol-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-amine [161];3-(7-(4-methyl-1H-imidazol-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [162];N,N-dimethyl-5-(3-(7-(4-methyl-1H-imidazol-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-amine [164];N-(5-(3-(7-(4-methyl-1H-imidazol-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-2-phenylacetamide [167];N-(5-(3-(7-(4-methyl-1H-imidazol-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)benzamide [168];N-isopropyl-5-(3-(7-(4-methyl-1H-imidazol-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-amine [169];N,N-dimethyl-1-(5-(3-(7-(4-methyl-1H-imidazol-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methanamine [170];3-(7-(4-methyl-1H-imidazol-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(pyrrolidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [171];3-(7-(4-methyl-1H-imidazol-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(piperidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [172];N-benzyl-1-(5-(3-(7-(4-methyl-1H-imidazol-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methanamine [180];1-cyclopentyl-N-((5-(3-(7-(4-methyl-1H-imidazol-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methyl)methanamine [181];5-(5-((3,3-difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-3-(7-(4-methyl-1H-imidazol-1-yl)-3H-imidazo [4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [182];5-(3-(7-(4-methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-amine [186];3-(7-(4-methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [187];3-(7-(4-methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(4-methylpyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [188];N,N-dimethyl-5-(3-(7-(4-methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-amine [190];N-(5-(3-(7-(4-methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-2-phenylacetamide [193];N-(5-(3-(7-(4-methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)benzamide [194];N-isopropyl-5-(3-(7-(4-methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-amine [195];N,N-dimethyl-1-(5-(3-(7-(4-methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methanamine [196];3-(7-(4-methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(pyrrolidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [197]; and3-(7-(4-methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(piperidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [198]; or a pharmaceutically acceptable salt thereof.

20. The method of claim 1, wherein the compound of Formula I is selected from the group consisting of: N-benzyl-1-(5-(3-(7-(4-methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methanamine [206];1-cyclopentyl-N-((5-(3-(7-(4-methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methyl)methanamine [207];5-(5-((3,3-difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-3-(7-(4-methylpiperazin-1-yl)-3H-imidazo [4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [208];5-(3-(3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-amine [211];3-(3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [212];5-(3-(3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)-N,N-dimethylpyridin-3-amine [214];N-(5-(3-(3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-2-phenylacetamide [216];N-(5-(3-(3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)benzamide [217];5-(3-(3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)-N-isopropylpyridin-3-amine [218];1-(5-(3-(3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-N,N-dimethylmethanamine [219];3-(3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(pyrrolidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [220];3-(3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(piperidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [221];1-(5-(3-(3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-N-benzylmethanamine [229];1-(5-(3-(3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-N-(cyclopentylmethyl)methanamine [230];5-(5-((3,3-difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-3-(3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [231];5-(3-(7-(thiophen-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-amine [235];5-(pyridin-3-yl)-3-(7-(thiophen-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [236];N,N-dimethyl-5-(3-(7-(thiophen-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-amine [239];2-phenyl-N-(5-(3-(7-(thiophen-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [242];N-(5-(3-(7-(thiophen-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)benzamide [243];N-isopropyl-5-(3-(7-(thiophen-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-amine [244];N,N-dimethyl-1-(5-(3-(7-(thiophen-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methanamine [245];5-(5-(pyrrolidin-1-ylmethyl)pyridin-3-yl)-3-(7-(thiophen-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [246];5-(5-(piperidin-1-ylmethyl)pyridin-3-yl)-3-(7-(thiophen-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [247];N-benzyl-1-(5-(3-(7-(thiophen-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methanamine [255];1-cyclopentyl-N-((5-(3-(7-(thiophen-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methyl)methanamine [256];5-(5-((3,3-difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-3-(7-(thiophen-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [257];5-(3-(7-(furan-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-amine [261];3-(7-(furan-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [262];5-(3-(7-(furan-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)-N,N-dimethylpyridin-3-amine [265];N-(5-(3-(7-(furan-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-2-phenylacetamide [268];N-(5-(3-(7-(furan-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)benzamide [269];5-(3-(7-(furan-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)-N-isopropylpyridin-3-amine [270];1-(5-(3-(7-(furan-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-N,N-dimethylmethanamine [271];3-(7-(furan-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(pyrrolidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [272];3-(7-(furan-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(piperidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [273];N-benzyl-1-(5-(3-(7-(furan-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methanamine [281];1-cyclopentyl-N-((5-(3-(7-(furan-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methyl)methanamine [282];5-(5-((3,3-difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-3-(7-(furan-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [283];5-(3-(7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-amine [287];5-(pyridin-3-yl)-3-(7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [288];N,N-dimethyl-5-(3-(7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-amine [291];2-phenyl-N-(5-(3-(7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [294];N-(5-(3-(7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)benzamide [295];N-isopropyl-5-(3-(7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-amine [296];N,N-dimethyl-1-(5-(3-(7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methanamine [297];5-(5-(pyrrolidin-1-ylmethyl)pyridin-3-yl)-3-(7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [298];5-(5-(piperidin-1-ylmethyl)pyridin-3-yl)-3-(7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [299];N-benzyl-1-(5-(3-(7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methanamine [307];1-cyclopentyl-N-((5-(3-(7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methyl)methanamine [308];5-(5-((3,3-difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-3-(7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [309];5-(3-(7-(5-fluorothiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-amine [313];3-(7-(5-fluorothiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [314];5-(3-(7-(5-fluorothiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)-N,N-dimethylpyridin-3-amine [317];N-(5-(3-(7-(5-fluorothiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-2-phenylacetamide [320];N-(5-(3-(7-(5-fluorothiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)benzamide [321];5-(3-(7-(5-fluorothiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)-N-isopropylpyridin-3-amine [322];1-(5-(3-(7-(5-fluorothiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-N,N-dimethylmethanamine [323];3-(7-(5-fluorothiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(pyrrolidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [324];3-(7-(5-fluorothiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(piperidin-1l-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [325];N-benzyl-1-(5-(3-(7-(5-fluorothiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methanamine [333];1-cyclopentyl-N-((5-(3-(7-(5-fluorothiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methyl)methanamine [334];5-(5-((3,3-difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-3-(7-(5-fluorothiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [335];5-(3-(7-(5-methylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-amine [339];3-(7-(5-methylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [340];N,N-dimethyl-5-(3-(7-(5-methylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-amine [343];N-(5-(3-(7-(5-methylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-2-phenylacetamide [346];N-(5-(3-(7-(5-methylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)benzamide [347];N-isopropyl-5-(3-(7-(5-methylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-amine [348];N,N-dimethyl-1-(5-(3-(7-(5-methylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methanamine [349];3-(7-(5-methylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(pyrrolidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [350];3-(7-(5-methylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(piperidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [351];N-benzyl-1-(5-(3-(7-(5-methylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methanamine [359];1-cyclopentyl-N-((5-(3-(7-(5-methylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methyl)methanamine [360];5-(5-((3,3-difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-3-(7-(5-methylthiophen-2-yl)-3H-imidazo [4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [361];1-(5-(2-(5-(5-aminopyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)thiophen-2-yl)ethanone [365];1-(5-(2-(5-(pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)thiophen-2-yl)ethanone [366];1-(5-(2-(5-(5-(dimethylamino)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)thiophen-2-yl)ethanone [369];N-(5-(3-(7-(5-acetylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-2-phenylacetamide [372];N-(5-(3-(7-(5-acetylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)benzamide [373];1-(5-(2-(5-(5-(isopropylamino)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)thiophen-2-yl)ethanone [374];1-(5-(2-(5-(5-((dimethylamino)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)thiophen-2-yl)ethanone [375];1-(5-(2-(5-(5-(pyrrolidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)thiophen-2-yl)ethanone [376];1-(5-(2-(5-(5-(piperidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)thiophen-2-yl)ethanone [377];1-(5-(2-(5-(5-((benzylamino)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)thiophen-2-yl)ethanone [385];1-(5-(2-(5-(5-(((cyclopentylmethyl)amino)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo [4,5-b]pyridin-7-yl)thiophen-2-yl)ethan-1-one [386];1-(5-(2-(5-(5-((3,3-difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo [4,5-b]pyridin-7-yl)thiophen-2-yl)ethanone [387];N-(3-(2-(5-(5-aminopyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)-5-fluorobenzyl)methanesulfonamide [391];N-(3-fluoro-5-(2-(5-(pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)benzyl)methanesulfonamide [392];N-(3-(2-(5-(5-(dimethylamino)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)-5-fluorobenzyl)methanesulfonamide [395];N-(5-(3-(7-(3-fluoro-5-(methylsulfonamidomethyl)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-2-phenylacetamide [398];N-(5-(3-(7-(3-fluoro-5-(methylsulfonamidomethyl)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)benzamide [399];N-(3-fluoro-5-(2-(5-(5-(isopropylamino)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)benzyl)methanesulfonamide [400];N-(3-(2-(5-(5-((dimethylamino)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)-5-fluorobenzyl)methanesulfonamide [401];N-(3-fluoro-5-(2-(5-(5-(pyrrolidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo [4,5-b]pyridin-7-yl)benzyl)methanesulfonamide [402];N-(3-fluoro-5-(2-(5-(5-(piperidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo [4,5-b]pyridin-7-yl)benzyl)methanesulfonamide [403];N-(3-(2-(5-(5-((benzylamino)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)-5-fluorobenzyl)methanesulfonamide [411];N-(3-(2-(5-(5-(((cyclopentylmethyl)amino)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo [4,5-b]pyridin-7-yl)-5-fluorobenzyl)methanesulfonamide [412];N-(3-(2-(5-(5-((3,3-difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo [4,5-b]pyridin-7-yl)-5-fluorobenzyl)methanesulfonamide [413];N-(3-(2-(5-(5-aminopyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)-5-fluorophenyl)-N2,N2-dimethylethane-1,2-diamine [417];N-(3-fluoro-5-(2-(5-(pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)phenyl)-N2,N2-dimethylethane-1,2-diamine [418];N-(3-(2-(5-(5-(dimethylamino)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)-5-fluorophenyl)-N2,N2-dimethylethane-1,2-diamine [421];N-(5-(3-(7-(3-((2-(dimethylamino)ethyl)amino)-5-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-2-phenylacetamide [424];N-(5-(3-(7-(3-((2-(dimethylamino)ethyl)amino)-5-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)benzamide [425];N-(3-fluoro-5-(2-(5-(5-(isopropylamino)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo [4,5-b]pyridin-7-yl)phenyl)-N2,N2-dimethylethane-1,2-diamine [426];N-(3-(2-(5-(5-((dimethylamino)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)-5-fluorophenyl)-N2,N2-dimethylethane-1,2-diamine [427];N1-(3-fluoro-5-(2-(5-(5-(pyrrolidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo [4,5-b]pyridin-7-yl)phenyl)-N2,N2-dimethylethane-1,2-diamine [428];N1-(3-fluoro-5-(2-(5-(5-(piperidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo [4,5-b]pyridin-7-yl)phenyl)-N2,N2-dimethylethane-1,2-diamine [429];N1-(3-(2-(5-(5-((benzylamino)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)-5-fluorophenyl)-N2,N2-dimethylethane-1,2-diamine [437];N1-(3-(2-(5-(5-(((cyclopentylmethyl)amino)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo [4,5-b]pyridin-7-yl)-5-fluorophenyl)-N2,N2-dimethylethane-1,2-diamine [438]; andN1-(3-(2-(5-(5-((3,3-difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo [4,5-b]pyridin-7-yl)-5-fluorophenyl)-N2,N2-dimethylethane-1,2-diamine [439]; or a pharmaceutically acceptable salt thereof.

21. The method of claim 1, wherein the compound of Formula I is selected from the group consisting of: 3-(7-(3-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(piperidin-4-yl)-1H-pyrazolo[3,4-b]pyridine [909];3-(7-(3-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(1,2,3,6-tetrahydropyridin-4-yl)-1H-pyrazolo[3,4-b]pyridine [910];3-(7-(3-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(1H-pyrazol-4-yl)-1H-pyrazolo[3,4-b]pyridine [911];1-(6-(3-(7-(3-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyrazin-2-yl)azetidin-3-amine [914];5-(5-(cyclohexyloxy)pyridin-3-yl)-3-(7-(3-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [915];3-(7-(3-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(piperidin-4-yloxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [916];N-(5-(3-(7-(3-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-2-(piperidin-4-yl)acetamide [917];3-(7-(3-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(2-(pyrrolidin-1-yl)ethoxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [918];2-((5-(3-(7-(3-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)oxy)-N,N-dimethylethan-1-amine [919];3-(7-(3-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-methoxypyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [920];5-(3-(7-(3-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-ol [921];5-(5-(benzyloxy)pyridin-3-yl)-3-(7-(3-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [922];2-cyclohexyl-N-(5-(3-(7-(3-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [923];3-(7-(3-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyridin-4-yl)-1H-pyrazolo[3,4-b]pyridine [924];3-(7-(3-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [925];3-(7-(3-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyrazin-2-yl)-1H-pyrazolo[3,4-b]pyridine [926];3-(7-(4-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(piperidin-4-yl)-1H-pyrazolo[3,4-b]pyridine [927];3-(7-(4-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(1,2,3,6-tetrahydropyridin-4-yl)-1H-pyrazolo[3,4-b]pyridine [928];3-(7-(4-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(1H-pyrazol-4-yl)-1H-pyrazolo[3,4-b]pyridine [929];1-(6-(3-(7-(4-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyrazin-2-yl)azetidin-3-amine [932];5-(5-(cyclohexyloxy)pyridin-3-yl)-3-(7-(4-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [933];3-(7-(4-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(piperidin-4-yloxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [934];N-(5-(3-(7-(4-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-2-(piperidin-4-yl)acetamide [935];3-(7-(4-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(2-(pyrrolidin-1-yl)ethoxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [936];2-((5-(3-(7-(4-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)oxy)-N,N-dimethylethan-1-amine [937];3-(7-(4-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-methoxypyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [938];5-(3-(7-(4-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-ol [939];5-(5-(benzyloxy)pyridin-3-yl)-3-(7-(4-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [940];2-cyclohexyl-N-(5-(3-(7-(4-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [941];3-(7-(4-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyridin-4-yl)-1H-pyrazolo[3,4-b]pyridine [942];3-(7-(4-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [943];3-(7-(4-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyrazin-2-yl)-1H-pyrazolo[3,4-b]pyridine [944];3-(7-(2-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(piperidin-4-yl)-1H-pyrazolo[3,4-b]pyridine [945];3-(7-(2-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(1,2,3,6-tetrahydropyridin-4-yl)-1H-pyrazolo[3,4-b]pyridine [946];3-(7-(2-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(1H-pyrazol-4-yl)-1H-pyrazolo[3,4-b]pyridine [947];1-(6-(3-(7-(2-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyrazin-2-yl)azetidin-3-amine [950];5-(5-(cyclohexyloxy)pyridin-3-yl)-3-(7-(2-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [951];3-(7-(2-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(piperidin-4-yloxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [952];N-(5-(3-(7-(2-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-2-(piperidin-4-yl)acetamide [953];3-(7-(2-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(2-(pyrrolidinn-1-yl)ethoxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [954];2-((5-(3-(7-(2-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)oxy)-N,N-dimethylethan-1-amine [955];3-(7-(2-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-methoxypyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [956];5-(3-(7-(2-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-ol [957];5-(5-(benzyloxy)pyridin-3-yl)-3-(7-(2-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [958];2-cyclohexyl-N-(5-(3-(7-(2-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [959];3-(7-(2-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyridin-4-yl)-1H-pyrazolo[3,4-b]pyridine [960];3-(7-(2-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [961];3-(7-(2-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyrazin-2-yl)-1H-pyrazolo[3,4-b]pyridine [962];5-(piperidin-4-yl)-3-(7-(pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [963];3-(7-(pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(1,2,3,6-tetrahydropyridin-4-yl)-1H-pyrazolo[3,4-b]pyridine [964];5-(1H-pyrazol-4-yl)-3-(7-(pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [965];1-(6-(3-(7-(pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyrazin-2-yl)azetidin-3-amine [968];5-(5-(cyclohexyloxy)pyridin-3-yl)-3-(7-(pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [969];5-(5-(piperidin-4-yloxy)pyridin-3-yl)-3-(7-(pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [970];2-(piperidin-4-yl)-N-(5-(3-(7-(pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [971];3-(7-(pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(2-(pyrrolidin-1-yl)ethoxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [972];N,N-dimethyl-2-((5-(3-(7-(pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)oxy)ethan-1-amine [973];5-(5-methoxypyridin-3-yl)-3-(7-(pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [974];5-(3-(7-(pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-ol [975];5-(5-(benzyloxy)pyridin-3-yl)-3-(7-(pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [976];2-cyclohexyl-N-(5-(3-(7-(pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [977];3-(7-(pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyridin-4-yl)-1H-pyrazolo[3,4-b]pyridine [978];5-(pyridin-2-yl)-3-(7-(pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [979];5-(pyrazin-2-yl)-3-(7-(pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [980];5-(piperidin-4-yl)-3-(7-(pyridin-4-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [981];3-(7-(pyridin-4-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(1,2,3,6-tetrahydropyridin-4-yl)-1H-pyrazolo[3,4-b]pyridine [982];5-(1H-pyrazol-4-yl)-3-(7-(pyridin-4-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [983];1-(6-(3-(7-(pyridin-4-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyrazin-2-yl)azetidin-3-amine [986];5-(5-(cyclohexyloxy)pyridin-3-yl)-3-(7-(pyridin-4-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [987];5-(5-(piperidin-4-yloxy)pyridin-3-yl)-3-(7-(pyridin-4-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [988];2-(piperidin-4-yl)-N-(5-(3-(7-(pyridin-4-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [989];3-(7-(pyridin-4-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(2-(pyrrolidin-1-yl)ethoxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [990];N,N-dimethyl-2-((5-(3-(7-(pyridin-4-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)oxy)ethan-1-amine [991];5-(5-methoxypyridin-3-yl)-3-(7-(pyridin-4-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [992];5-(3-(7-(pyridin-4-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-ol [993];5-(5-(benzyloxy)pyridin-3-yl)-3-(7-(pyridin-4-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [994];2-cyclohexyl-N-(5-(3-(7-(pyridin-4-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [995];5-(pyridin-4-yl)-3-(7-(pyridin-4-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [996];5-(pyridin-2-yl)-3-(7-(pyridin-4-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [997];5-(pyrazin-2-yl)-3-(7-(pyridin-4-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [998];5-(piperidin-4-yl)-3-(7-(pyridin-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [999]; and3-(7-(pyridin-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(1,2,3,6-tetrahydropyridin-4-yl)-1H-pyrazolo[3,4-b]pyridine [1000]; or a pharmaceutically acceptable salt thereof.

22. The compound of claim 1, wherein the compound of Formula I is selected from the group consisting of: 5-(1H-pyrazol-4-yl)-3-(7-(pyridin-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1001];1-(6-(3-(7-(pyridin-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyrazin-2-yl)azetidin-3-amine [1004];5-(5-(cyclohexyloxy)pyridin-3-yl)-3-(7-(pyridin-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1005];5-(5-(piperidin-4-yloxy)pyridin-3-yl)-3-(7-(pyridin-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1006];2-(piperidin-4-yl)-N-(5-(3-(7-(pyridin-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [1007];3-(7-(pyridin-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(2-(pyrrolidin-1-yl)ethoxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [1008];N,N-dimethyl-2-((5-(3-(7-(pyridin-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)oxy)ethan-1-amine [1009];5-(5-methoxypyridin-3-yl)-3-(7-(pyridin-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1010];5-(3-(7-(pyridin-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-ol [1011];5-(5-(benzyloxy)pyridin-3-yl)-3-(7-(pyridin-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1012];2-cyclohexyl-N-(5-(3-(7-(pyridin-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [1013];3-(7-(pyridin-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyridin-4-yl)-1H-pyrazolo[3,4-b]pyridine [1014];5-(pyridin-2-yl)-3-(7-(pyridin-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1015];5-(pyrazin-2-yl)-3-(7-(pyridin-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1016];3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(piperidin-4-yl)-1H-pyrazolo[3,4-b]pyridine [1017];3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(1,2,3,6-tetrahydropyridin-4-yl)-1H-pyrazolo[3,4-b]pyridine [1018];3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(1H-pyrazol-4-yl)-1H-pyrazolo[3,4-b]pyridine [1019];5-(1-methyl-1H-pyrazol-4-yl)-3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1020];5-(1,2-dimethyl-1H-imidazol-5-yl)-3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1021];1-(6-(3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyrazin-2-yl)azetidin-3-amine [1022];5-(5-(cyclohexyloxy)pyridin-3-yl)-3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1023];3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(piperidin-4-yloxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [1024];N-(5-(3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-2-(piperidin-4-yl)acetamide [1025];3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(2-(pyrrolidin-1-yl)ethoxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [1026];N,N-dimethyl-2-((5-(3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)oxy)ethan-1-amine [1027];5-(5-methoxypyridin-3-yl)-3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1028];5-(3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-ol [1029];5-(5-(benzyloxy)pyridin-3-yl)-3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1030];2-cyclohexyl-N-(5-(3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [1031];3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyridin-4-yl)-1H-pyrazolo[3,4-b]pyridine [1032];3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1033];3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyrazin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1034];3-(7-(4-methyl-1H-imidazol-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(piperidin-4-yl)-1H-pyrazolo[3,4-b]pyridine [1035];3-(7-(4-methyl-1H-imidazol-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(1,2,3,6-tetrahydropyridin-4-yl)-1H-pyrazolo[3,4-b]pyridine [1036];3-(7-(4-methyl-1H-imidazol-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(1H-pyrazol-4-yl)-1H-pyrazolo[3,4-b]pyridine [1037];1-(6-(3-(7-(4-methyl-1H-imidazol-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyrazin-2-yl)azetidin-3-amine [1040];5-(5-(cyclohexyloxy)pyridin-3-yl)-3-(7-(4-methyl-1H-imidazol-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1041];3-(7-(4-methyl-1H-imidazol-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(piperidin-4-yloxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [1042];N-(5-(3-(7-(4-methyl-1H-imidazol-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-2-(piperidin-4-yl)acetamide [1043];3-(7-(4-methyl-1H-imidazol-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(2-(pyrrolidin-1-yl)ethoxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [1044];N,N-dimethyl-2-((5-(3-(7-(4-methyl-1H-imidazol-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)oxy)ethan-1-amine [1045];5-(5-methoxypyridin-3-yl)-3-(7-(4-methyl-1H-imidazol-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1046];5-(3-(7-(4-methyl-1H-imidazol-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazol-yl)-[3,4-b]pyridin-5-yl)pyridin-3-ol [1047];5-(5-(benzyloxy)pyridin-3-yl)-3-(7-(4-methyl-1H-imidazol-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1048];2-cyclohexyl-N-(5-(3-(7-(4-methyl-1H-imidazol-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [1049];3-(7-(4-methyl-1H-imidazol-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyridin-4-yl)-1H-pyrazolo[3,4-b]pyridine [1050];3-(7-(4-methyl-1H-imidazol-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1051];3-(7-(4-methyl-1H-imidazol-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyrazin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1052];3-(7-(4-methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(piperidin-4-yl)-1H-pyrazolo[3,4-b]pyridine [1053];3-(7-(4-methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(1,2,3,6-tetrahydropyridin-4-yl)-1H-pyrazolo[3,4-b]pyridine [1054];3-(7-(4-methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(1H-pyrazol-4-yl)-1H-pyrazolo[3,4-b]pyridine [1055];1-(6-(3-(7-(4-methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyrazin-2-yl)azetidin-3-amine [1058];5-(5-(cyclohexyloxy)pyridin-3-yl)-3-(7-(4-methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1059];3-(7-(4-methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(piperidin-4-yloxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [1060];N-(5-(3-(7-(4-methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-2-(piperidin-4-yl)acetamide [1061];3-(7-(4-methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(2-(pyrrolidin-1-yl)ethoxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [1062];N,N-dimethyl-2-((5-(3-(7-(4-methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)oxy)ethan-1-amine [1063];5-(5-methoxypyridin-3-yl)-3-(7-(4-methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1064];5-(3-(7-(4-methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-ol [1065];5-(5-(benzyloxy)pyridin-3-yl)-3-(7-(4-methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1066];2-cyclohexyl-N-(5-(3-(7-(4-methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [1067];3-(7-(4-methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyridin-4-yl)-1H-pyrazolo[3,4-b]pyridine [1068];3-(7-(4-methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1069];3-(7-(4-methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyrazin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1070];3-(3H-imidazo[4,5-b]pyridin-2-yl)-5-(piperidin-4-yl)-1H-pyrazolo[3,4-b]pyridine [1071];3-(3H-imidazo[4,5-b]pyridin-2-yl)-5-(1,2,3,6-tetrahydropyridin-4-yl)-1H-pyrazolo[3,4-b]pyridine [1072];3-(3H-imidazo[4,5-b]pyridin-2-yl)-5-(1H-pyrazol-4-yl)-1H-pyrazolo[3,4-b]pyridine [1073];1-(6-(3-(3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyrazin-2-yl)azetidin-3-amine [1076];5-(5-(cyclohexyloxy)pyridin-3-yl)-3-(3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1077];3-(3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(piperidin-4-yloxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [1078];N-(5-(3-(3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-2-(piperidin-4-yl)acetamide [1079];3-(3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(2-(pyrrolidin-1-yl)ethoxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [1080];2-((5-(3-(3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)oxy)-N,N-dimethylethan-1-amine [1081];3-(3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-methoxypyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [1082];5-(3-(3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-ol [1083];5-(5-(benzyloxy)pyridin-3-yl)-3-(3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1084];N-(5-(3-(3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-2-cyclohexylacetamide [1085];3-(3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyridin-4-yl)-1H-pyrazolo[3,4-b]pyridine [1086];3-(3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1087];3-(3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyrazin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1088];5-(piperidin-4-yl)-3-(7-(thiophen-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1089];5-(1,2,3,6-tetrahydropyridin-4-yl)-3-(7-(thiophen-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1090];5-(1H-pyrazol-4-yl)-3-(7-(thiophen-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine[1091];1-(6-(3-(7-(thiophen-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyrazin-2-yl)azetidin-3-amine [1094];5-(5-(cyclohexyloxy)pyridin-3-yl)-3-(7-(thiophen-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1095];5-(5-(piperidin-4-yloxy)pyridin-3-yl)-3-(7-(thiophen-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1096];2-(piperidin-4-yl)-N-(5-(3-(7-(thiophen-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [1097];5-(5-(2-(pyrrolidin-1-yl)ethoxy)pyridin-3-yl)-3-(7-(thiophen-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1098];N,N-dimethyl-2-((5-(3-(7-(thiophen-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)oxy)ethan-1-amine [1099];5-(5-methoxypyridin-3-yl)-3-(7-(thiophen-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1100];5-(3-(7-(thiophen-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-ol [1101];5-(5-(benzyloxy)pyridin-3-yl)-3-(7-(thiophen-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1102];2-cyclohexyl-N-(5-(3-(7-(thiophen-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [1103];5-(pyridin-4-yl)-3-(7-(thiophen-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1104];5-(pyridin-2-yl)-3-(7-(thiophen-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1105];5-(pyrazin-2-yl)-3-(7-(thiophen-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1106];3-(7-(furan-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(piperidin-4-yl)-1H-pyrazolo [3,4-b]pyridine [1107];3-(7-(furan-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(1,2,3,6-tetrahydropyridin-4-yl)-1H-pyrazolo[3,4-b]pyridine [1108];3-(7-(furan-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(1H-pyrazol-4-yl)-1H-pyrazolo[3,4-b]pyridine [1109];1-(6-(3-(7-(furan-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyrazin-2-yl)azetidin-3-amine [1112];5-(5-(cyclohexyloxy)pyridin-3-yl)-3-(7-(furan-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1113];3-(7-(furan-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(piperidin-4-yloxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [1114];N-(5-(3-(7-(furan-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-2-(piperidin-4-yl)acetamide [1115];3-(7-(furan-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(2-(pyrrolidin-1-yl)ethoxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [1116];2-((5-(3-(7-(furan-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)oxy)-N,N-dimethylethan-1-amine [1117];3-(7-(furan-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-methoxypyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [1118];5-(3-(7-(furan-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-ol [1119];5-(5-(benzyloxy)pyridin-3-yl)-3-(7-(furan-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1120];2-cyclohexyl-N-(5-(3-(7-(furan-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [1121];3-(7-(furan-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyridin-4-yl)-1H-pyrazolo[3,4-b]pyridine [1122];3-(7-(furan-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1123];3-(7-(furan-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyrazin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1124];5-(piperidin-4-yl)-3-(7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1125];5-(1,2,3,6-tetrahydropyridin-4-yl)-3-(7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1126];5-(1H-pyrazol-4-yl)-3-(7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1127];1 (6-(3-(7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyrazin-2-yl)azetidin-3-amine [1130];5-(5-(cyclohexyloxy)pyridin-3-yl)-3-(7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo [3,4-b]pyridine [1131];5-(5-(piperidin-4-yloxy)pyridin-3-yl)-3-(7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1132];2-(piperidin-4-yl)-N-(5-(3-(7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [1133];5-(5-(2-(pyrrolidin-1-yl)ethoxy)pyridin-3-yl)-3-(7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1134];N,N-dimethyl-2-((5-(3-(7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)oxy)ethan-1-amine [1135];5-(5-methoxypyridin-3-yl)-3-(7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1136];5-(3-(7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-ol [1137];5-(5-(benzyloxy)pyridin-3-yl)-3-(7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1138];2-cyclohexyl-N-(5-(3-(7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [1139];5-(pyridin-4-yl)-3-(7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1140];5-(pyridin-2-yl)-3-(7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1141];5-(pyrazin-2-yl)-3-(7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1142];3-(7-(5-fluorothiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(piperidin-4-yl)-1H-pyrazolo[3,4-b]pyridine [1143];3-(7-(5-fluorothiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(1,2,3,6-tetrahydropyridin-4-yl)-1H-pyrazolo[3,4-b]pyridine [1144];3-(7-(5-fluorothiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(1H-pyrazol-4-yl)-1H-pyrazolo[3,4-b]pyridine [1145];1-(6-(3-(7-(5-fluorothiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyrazin-2-yl)azetidin-3-amine [1148];5-(5-(cyclohexyloxy)pyridin-3-yl)-3-(7-(5-fluorothiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1149];3-(7-(5-fluorothiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(piperidin-4-yloxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [1150];N-(5-(3-(7-(5-fluorothiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-2-(piperidin-4-yl)acetamide [1151];3-(7-(5-fluorothiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(2-(pyrrolidin-1-yl)ethoxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [1152];2-((5-(3-(7-(5-fluorothiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)oxy)-N,N-dimethylethan-1-amine [1153];3-(7-(5-fluorothiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-methoxypyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [1154];5-(3-(7-(5-fluorothiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-ol [1155];5-(5-(benzyloxy)pyridin-3-yl)-3-(7-(5-fluorothiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1156];2-cyclohexyl-N-(5-(3-(7-(5-fluorothiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [1157];3-(7-(5-fluorothiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyridin-4-yl)-1H-pyrazolo[3,4-b]pyridine [1158];3-(7-(5-fluorothiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1159];3-(7-(5-fluorothiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyrazin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1160];3-(7-(5-methylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(piperidin-4-yl)-1H-pyrazolo[3,4-b]pyridine [1161];3-(7-(5-methylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(1,2,3,6-tetrahydropyridin-4-yl)-1H-pyrazolo[3,4-b]pyridine [1162];3-(7-(5-methylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(1H-pyrazol-4-yl)-1H-pyrazolo[3,4-b]pyridine [1163];1-(6-(3-(7-(5-methylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyrazin-2-yl)azetidin-3-amine [1166];5-(5-(cyclohexyloxy)pyridin-3-yl)-3-(7-(5-methylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1167];3-(7-(5-methylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(piperidin-4-yloxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [1168];N-(5-(3-(7-(5-methylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-2-(piperidin-4-yl)acetamide [1169];3-(7-(5-methylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(2-(pyrrolidin-1-yl)ethoxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [1170];N,N-dimethyl-2-((5-(3-(7-(5-methylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)oxy)ethan-1-amine [1171];5-(5-methoxypyridin-3-yl)-3-(7-(5-methylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1172];5-(3-(7-(5-methylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-ol [1173];5-(5-(benzyloxy)pyridin-3-yl)-3-(7-(5-methylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1174];2-cyclohexyl-N-(5-(3-(7-(5-methylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [1175];3-(7-(5-methylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyridin-4-yl)-1H-pyrazolo[3,4-b]pyridine [1176];3-(7-(5-methylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1177];3-(7-(5-methylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyrazin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1178];1-(5-(2-(5-(piperidin-4-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)thiophen-2-yl)ethan-1-one [1179];1-(5-(2-(5-(1,2,3,6-tetrahydropyridin-4-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)thiophen-2-yl)ethan-1-one [1180];1-(5-(2-(5-(1H-pyrazol-4-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)thiophen-2-yl)ethan-1-one [1181];1-(5-(2-(5-(6-(3-aminoazetidin-1-yl)pyrazin-2-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo [4,5-b]pyridin-7-yl)thiophen-2-yl)ethan-1-one [1184];1-(5-(2-(5-(5-(cyclohexyloxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)thiophen-2-yl)ethan-1-one [1185];1-(5-(2-(5-(5-(piperidin-4-yloxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)thiophen-2-yl)ethan-1-one [1186];N-(5-(3-(7-(5-acetylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-2-(piperidin-4-yl)acetamide [1187];1-(5-(2-(5-(5-(2-(pyrrolidin-1-yl)ethoxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo [4,5-b]pyridin-7-yl)thiophen-2-yl)ethan-1-one [1188];1-(5-(2-(5-(5-(2-(dimethylamino)ethoxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo [4,5-b]pyridin-7-yl)thiophen-2-yl)ethan-1-one [1189];1-(5-(2-(5-(5-methoxypyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)thiophen-2-yl)ethan-1-one [1190];1-(5-(2-(5-(5-hydroxypyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)thiophen-2-yl)ethan-1-one [1191];1-(5-(2-(5-(5-(benzyloxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)thiophen-2-yl)ethan-1-one [1192];N-(5-(3-(7-(5-acetylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-2-cyclohexylacetamide [1193];1-(5-(2-(5-(pyridin-4-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)thiophen-2-yl)ethan-1-one [1194];1-(5-(2-(5-(pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)thiophen-2-yl)ethan-1-one [1195];1-(5-(2-(5-(pyrazin-2-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)thiophen-2-yl)ethan-1-one [1196];N-(3-fluoro-5-(2-(5-(piperidin-4-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)benzyl)methanesulfonamide [1197];N-(3-fluoro-5-(2-(5-(1,2,3,6-tetrahydropyridin-4-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo [4,5-b]pyridin-7-yl)benzyl)methanesulfonamide [1198]; andN-(3-(2-(5-(1H-pyrazol-4-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)-5-fluorobenzyl)methanesulfonamide [1199]; or a pharmaceutically acceptable salt thereof.

23. The method of claim 1, wherein the compound of Formula I is selected from the group consisting of: N-(3-(2-(5-(6-(3-aminoazetidin-1-yl)pyrazin-2-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)-5-fluorobenzyl)methanesulfonamide [1202];N-(3-(2-(5-(5-(cyclohexyloxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)-5-fluorobenzyl)methanesulfonamide [1203];N-(3-fluoro-5-(2-(5-(5-(piperidin-4-yloxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo [4,5-b]pyridin-7-yl)benzyl)methanesulfonamide [1204];N-(5-(3-(7-(3-fluoro-5-(methylsulfonamidomethyl)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo [3,4-b]pyridin-5-yl)pyridin-3-yl)-2-(piperidin-4-yl)acetamide [1205];N-(3-fluoro-5-(2-(5-(5-(2-(pyrrolidin-1-yl)ethoxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo [4,5-b]pyridin-7-yl)benzyl)methanesulfonamide [1206];N-(3-(2-(5-(5-(2-(dimethylamino)ethoxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo [4,5-b]pyridin-7-yl)-5-fluorobenzyl)methanesulfonamide [1207];N-(3-fluoro-5-(2-(5-(5-methoxypyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)benzyl)methanesulfonamide [1208];N-(3-fluoro-5-(2-(5-(5-hydroxypyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)benzyl)methanesulfonamide [1209];N-(3-(2-(5-(5-(benzyloxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)-5-fluorobenzyl)methanesulfonamide [1210];2-cyclohexyl-N-(5-(3-(7-(3-fluoro-5-(methylsulfonamidomethyl)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [1211];N-(3-fluoro-5-(2-(5-(pyridin-4-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)benzyl)methanesulfonamide [1212];N-(3-fluoro-5-(2-(5-(pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)benzyl)methanesulfonamide [1213];N-(3-fluoro-5-(2-(5-(pyrazin-2-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)benzyl)methanesulfonamide [1214];N1-(3-fluoro-5-(2-(5-(piperidin-4-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)phenyl)-N2,N2-dimethylethane-1,2-diamine [1215];N1-(3-fluoro-5-(2-(5-(1,2,3,6-tetrahydropyridin-4-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo [4,5-b]pyridin-7-yl)phenyl)-N2,N2-dimethylethane-1,2-diamine [1216];N1-(3-(2-(5-(1H-pyrazol-4-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)-5-fluorophenyl)-N2,N2-dimethylethane-1,2-diamine [1217];N1-(3-(2-(5-(6-(3-aminoazetidin-1-yl)pyrazin-2-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo [4,5-b]pyridin-7-yl)-5-fluorophenyl)-N2,N2-dimethylethane-1,2-diamine [1220];N1-(3-(2-(5-(5-(cyclohexyloxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo [4,5-b]pyridin-7-yl)-5-fluorophenyl)-N2,N2-dimethylethane-1,2-diamine [1221];N1-(3-fluoro-5-(2-(5-(5-(piperidin-4-yloxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo [4,5-b]pyridin-7-yl)phenyl)-N2,N2-dimethylethane-1,2-diamine [1222];N-(5-(3-(7-(3-((2-(dimethylamino)ethyl)amino)-5-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-2-(piperidin-4-yl)acetamide [1223];N1-(3-fluoro-5-(2-(5-(5-(2-(pyrrolidin-1-yl)ethoxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo [4,5-b]pyridin-7-yl)phenyl)-N2,N2-dimethylethane-1,2-diamine [1224];N1-(3-(2-(5-(5-(2-(dimethylamino)ethoxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo [4,5-b]pyridin-7-yl)-5-fluorophenyl)-N2,N2-dimethylethane-1,2-diamine [1225];N1-(3-fluoro-5-(2-(5-(5-methoxypyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo [4,5-b]pyridin-7-yl)phenyl)-N2,N2-dimethylethane-1,2-diamine [1226];5-(3-(7-(3-((2-(dimethylamino)ethyl)amino)-5-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-ol [1227];N1-(3-(2-(5-(5-(benzyloxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)-5-fluorophenyl)-N2,N2-dimethylethane-1,2-diamine [1228];2-cyclohexyl-N-(5-(3-(7-(3-((2-(dimethylamino)ethyl)amino)-5-fluorophenyl)-3H-imidazo [4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [1229];N1-(3-fluoro-5-(2-(5-(pyridin-4-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)phenyl)-N2,N2-dimethylethane-1,2-diamine [1230];N1-(3-fluoro-5-(2-(5-(pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)phenyl)-N2,N2-dimethylethane-1,2-diamine [1231];N1-(3-fluoro-5-(2-(5-(pyrazin-2-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)phenyl)-N2,N2-dimethylethane-1,2-diamine [1232];5-(3-(7-(3-(2-(dimethylamino)ethoxy)-5-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-amine [1235];2-(3-fluoro-5-(2-(5-(pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)phenoxy)-N,N-dimethylethan-1-amine [1236];5-(3-(7-(3-(2-(dimethylamino)ethoxy)-5-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)-N,N-dimethylpyridin-3-amine [1239];N-(5-(3-(7-(3-(2-(dimethylamino)ethoxy)-5-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-2-phenylacetamide [1242];N-(5-(3-(7-(3-(2-(dimethylamino)ethoxy)-5-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)benzamide [1243];5-(3-(7-(3-(2-(dimethylamino)ethoxy)-5-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)-N-isopropylpyridin-3-amine [1244];2-(3-(2-(5-(5-((dimethylamino)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo [4,5-b]pyridin-7-yl)-5-fluorophenoxy)-N,N-dimethylethan-1-amine [1245];2-(3-fluoro-5-(2-(5-(5-(pyrrolidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo [4,5-b]pyridin-7-yl)phenoxy)-N,N-dimethylethan-1-amine [1246];2-(3-fluoro-5-(2-(5-(5-(piperidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo [4,5-b]pyridin-7-yl)phenoxy)-N,N-dimethylethan-1-amine [1247];2-(3-(2-(5-(5-((benzylamino)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)-5-fluorophenoxy)-N,N-dimethylethan-1-amine [1255];2-(3-(2-(5-(5-(((cyclopentylmethyl)amino)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo [4,5-b]pyridin-7-yl)-5-fluorophenoxy)-N,N-dimethylethan-1-amine [1256];2-(3-(2-(5-(5-((3,3-difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo [4,5-b]pyridin-7-yl)-5-fluorophenoxy)-N,N-dimethylethan-1-amine [1257];2-(3-fluoro-5-(2-(5-(pyrimidin-5-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)phenoxy)-N,N-dimethylethan-1-amine [1258];2-(3-fluoro-5-(2-(5-(pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)phenoxy)-N,N-dimethylethan-1-amine [1259];2-(3-fluoro-5-(2-(5-(piperidin-4-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)phenoxy)-N,N-dimethylethan-1-amine [1260];2-(3-fluoro-5-(2-(5-(1,2,3,6-tetrahydropyridin-4-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo [4,5-b]pyridin-7-yl)phenoxy)-N,N-dimethylethan-1-amine [1261];2-(3-(2-(5-(1H-pyrazol-4-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)-5-fluorophenoxy)-N,N-dimethylethan-1-amine [1262];1-(6-(3-(7-(3-(2-(dimethylamino)ethoxy)-5-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyrazin-2-yl)azetidin-3-amine [1265];2-(3-(2-(5-(5-(cyclohexyloxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)-5-fluorophenoxy)-N,N-dimethylethan-1-amine [1266];2-(3-fluoro-5-(2-(5-(5-(piperidin-4-yloxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo [4,5-b]pyridin-7-yl)phenoxy)-N,N-dimethylethan-1-amine [1267];N-(5-(3-(7-(3-(2-(dimethylamino)ethoxy)-5-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-2-(piperidin-4-yl)acetamide [1268];2-(3-fluoro-5-(2-(5-(5-(2-(pyrrolidin-1-yl)ethoxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo [4,5-b]pyridin-7-yl)phenoxy)-N,N-dimethylethan-1-amine [1269];2-((5-(3-(7-(3-(2-(dimethylamino)ethoxy)-5-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)oxy)-N,N-dimethylethan-1-amine [1270];2-(3-fluoro-5-(2-(5-(5-methoxypyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)phenoxy)-N,N-dimethylethan-1-amine [1271];5-(3-(7-(3-(2-(dimethylamino)ethoxy)-5-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-ol [1272];2-(3-(2-(5-(5-(benzyloxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)-5-fluorophenoxy)-N,N-dimethylethan-1-amine [1273];2-cyclohexyl-N-(5-(3-(7-(3-(2-(dimethylamino)ethoxy)-5-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [1274];2-(3-fluoro-5-(2-(5-(pyridin-4-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)phenoxy)-N,N-dimethylethan-1-amine [1275];2-(3-fluoro-5-(2-(5-(pyrazin-2-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)phenoxy)-N,N-dimethylethan-1-amine [1276];5-(3-(7-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-amine [1279];3-(7-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [1280];5-(3-(7-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)-N,N-dimethylpyridin-3-amine [1283];N-(5-(3-(7-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-2-phenylacetamide [1286];N-(5-(3-(7-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)benzamide [1287];5-(3-(7-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)-N-isopropylpyridin-3-amine [1288];1-(5-(3-(7-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-N,N-dimethylmethanamine [1289];3-(7-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(pyrrolidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [1290];3-(7-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(piperidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [1291];N-benzyl-1-(5-(3-(7-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methanamine [1299];1-cyclopentyl-N-((5-(3-(7-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methyl)methanamine [1300];5-(5-((3,3-difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-3-(7-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1301];3-(7-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyrimidin-5-yl)-1H-pyrazolo[3,4-b]pyridine [1302];3-(7-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1303];3-(7-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(piperidin-4-yl)-1H-pyrazolo[3,4-b]pyridine [1304];3-(7-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(1,2,3,6-tetrahydropyridin-4-yl)-1H-pyrazolo[3,4-b]pyridine [1305];3-(7-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(H-pyrazol-4-yl)-1H-pyrazolo[3,4-b]pyridine [1306];1-(6-(3-(7-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyrazin-2-yl)azetidin-3-amine [1309];5-(5-(cyclohexyloxy)pyridin-3-yl)-3-(7-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1310];3-(7-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(piperidin-4-yloxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [1311];N-(5-(3-(7-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo [3,4-b]pyridin-5-yl)pyridin-3-yl)-2-(piperidin-4-yl)acetamide [1312];3-(7-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(2-(pyrrolidin-1-yl)ethoxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [1313];2-((5-(3-(7-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)oxy)-N,N-dimethylethan-1-amine [1314];3-(7-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-methoxypyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [1315];5-(3-(7-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-ol [1316];5-(5-(benzyloxy)pyridin-3-yl)-3-(7-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-3H-imidazo [4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1317];2-cyclohexyl-N-(5-(3-(7-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [1318];3-(7-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyridin-4-yl)-1H-pyrazolo[3,4-b]pyridine [1319];3-(7-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyrazin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1320];3-(2-(5-(5-aminopyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)-5-fluorophenol [1323];3-fluoro-5-(2-(5-(pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)phenol [1324];3-(2-(5-(5-(dimethylamino)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)-5-fluorophenol [1327];N-(5-(3-(7-(3-fluoro-5-hydroxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-2-phenylacetamide [1330];N-(5-(3-(7-(3-fluoro-5-hydroxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)benzamide [1331];3-fluoro-5-(2-(5-(5-(isopropylamino)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)phenol [1332];3-(2-(5-(5-((dimethylamino)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)-5-fluorophenol [1333];3-fluoro-5-(2-(5-(5-(pyrrolidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo [4,5-b]pyridin-7-yl)phenol [1334];3-fluoro-5-(2-(5-(5-(piperidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo [4,5-b]pyridin-7-yl)phenol [1335];3-(2-(5-(5-((benzylamino)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)-5-fluorophenol [1343];3-(2-(5-(5-(((cyclopentylmethyl)amino)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo [4,5-b]pyridin-7-yl)-5-fluorophenol [1344];3-(2-(5-(5-((3,3-difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo [4,5-b]pyridin-7-yl)-5-fluorophenol [1345];3-fluoro-5-(2-(5-(pyrimidin-5-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)phenol [1346];3-fluoro-5-(2-(5-(pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)phenol [1347];3-fluoro-5-(2-(5-(piperidin-4-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)phenol [1348];3-fluoro-5-(2-(5-(1,2,3,6-tetrahydropyridin-4-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo [4,5-b]pyridin-7-yl)phenol [1349];3-(2-(5-(1H-pyrazol-4-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)-5-fluorophenol [1350];3-(2-(5-(6-(3-aminoazetidin-1-yl)pyrazin-2-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)-5-fluorophenol [1353];3-(2-(5-(5-(cyclohexyloxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)-5-fluorophenol [1354];3-fluoro-5-(2-(5-(5-(piperidin-4-yloxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo [4,5-b]pyridin-7-yl)phenol [1355];N-(5-(3-(7-(3-fluoro-5-hydroxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-2-(piperidin-4-yl)acetamide [1356];3-fluoro-5-(2-(5-(5-(2-(pyrrolidin-1-yl)ethoxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo [4,5-b]pyridin-7-yl)phenol [1357];3-(2-(5-(5-(2-(dimethylamino)ethoxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)-5-fluorophenol [1358];3-fluoro-5-(2-(5-(5-methoxypyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)phenol [1359];5-(3-(7-(3-fluoro-5-hydroxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-ol [1360];3-(2-(5-(5-(benzyloxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)-5-fluorophenol [1361];2-cyclohexyl-N-(5-(3-(7-(3-fluoro-5-hydroxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [1362];3-fluoro-5-(2-(5-(pyridin-4-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)phenol [1363];3-fluoro-5-(2-(5-(pyrazin-2-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)phenol [1364];5-(3-(7-(3-fluoro-5-methoxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-amine [1367];3-(7-(3-fluoro-5-methoxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [1368];5-(3-(7-(3-fluoro-5-methoxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)-N,N-dimethylpyridin-3-amine [1371];N-(5-(3-(7-(3-fluoro-5-methoxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-2-phenylacetamide [1374];N-(5-(3-(7-(3-fluoro-5-methoxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)benzamide [1375];5-(3-(7-(3-fluoro-5-methoxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)-N-isopropylpyridin-3-amine [1376];1-(5-(3-(7-(3-fluoro-5-methoxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-N,N-dimethylmethanamine [1377];3-(7-(3-fluoro-5-methoxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(pyrrolidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [1378];3-(7-(3-fluoro-5-methoxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(piperidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [1379];N-benzyl-1-(5-(3-(7-(3-fluoro-5-methoxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methanamine [1387];1-cyclopentyl-N-((5-(3-(7-(3-fluoro-5-methoxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methyl)methanamine [1388];5-(5-((3,3-difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-3-(7-(3-fluoro-5-methoxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1389];3-(7-(3-fluoro-5-methoxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyrimidin-5-yl)-1H-pyrazolo[3,4-b]pyridine [1390];3-(7-(3-fluoro-5-methoxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1391];3-(7-(3-fluoro-5-methoxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(piperidin-4-yl)-1H-pyrazolo[3,4-b]pyridine [1392];3-(7-(3-fluoro-5-methoxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(1,2,3,6-tetrahydropyridin-4-yl)-1H-pyrazolo[3,4-b]pyridine [1393];3-(7-(3-fluoro-5-methoxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(1H-pyrazol-4-yl)-1H-pyrazolo[3,4-b]pyridine [1394];1-(6-(3-(7-(3-fluoro-5-methoxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyrazin-2-yl)azetidin-3-amine [1397];5-(5-(cyclohexyloxy)pyridin-3-yl)-3-(7-(3-fluoro-5-methoxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1398];3-(7-(3-fluoro-5-methoxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(piperidin-4-yloxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [1399]; andN-(5-(3-(7-(3-fluoro-5-methoxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-2-(piperidin-4-yl)acetamide [1400]; or a pharmaceutically acceptable salt thereof.

24. The method of claim 1, wherein the compound of Formula I is selected from the group consisting of: 3-(7-(3-fluoro-5-methoxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-(2-(pyrrolidin-1-yl)ethoxy)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [1401];2-((5-(3-(7-(3-fluoro-5-methoxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)oxy)-N,N-dimethylethan-1-amine [1402];3-(7-(3-fluoro-5-methoxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-methoxypyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [1403];5-(3-(7-(3-fluoro-5-methoxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-ol [1404];5-(5-(benzyloxy)pyridin-3-yl)-3-(7-(3-fluoro-5-methoxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1405];2-cyclohexyl-N-(5-(3-(7-(3-fluoro-5-methoxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [1406];3-(7-(3-fluoro-5-methoxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyridin-4-yl)-1H-pyrazolo[3,4-b]pyridine [1407];3-(7-(3-fluoro-5-methoxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyrazin-2-yl)-1H-pyrazolo [3,4-b]pyridine [1408];2-(dimethylamino)-N-(5-(3-(7-(3-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [1409];2-(dimethylamino)-N-(5-(3-(7-(4-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [1410];2-(dimethylamino)-N-(5-(3-(7-(2-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [1411];2-(dimethylamino)-N-(5-(3-(7-(pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [1412];2-(dimethylamino)-N-(5-(3-(7-(pyridin-4-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [1413];2-(dimethylamino)-N-(5-(3-(7-(pyridin-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [1414];2-(dimethylamino)-N-(5-(3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [1415];2-(dimethylamino)-N-(5-(3-(7-(4-methyl-1H-imidazol-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo [3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [1416];2-(dimethylamino)-N-(5-(3-(7-(4-methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo [3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [1417];N-(5-(3-(3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-2-(dimethylamino)acetamide [1418];2-(dimethylamino)-N-(5-(3-(7-(thiophen-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [1419];2-(dimethylamino)-N-(5-(3-(7-(furan-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [1420];2-(dimethylamino)-N-(5-(3-(7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [1421];2-(dimethylamino)-N-(5-(3-(7-(5-fluorothiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [1422];2-(dimethylamino)-N-(5-(3-(7-(5-methylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [1423];N-(5-(3-(7-(5-acetylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-2-(dimethylamino)acetamide [1424];2-(dimethylamino)-N-(5-(3-(7-(3-fluoro-5-(methylsulfonamidomethyl)phenyl)-3H-imidazo [4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [1425];2-(dimethylamino)-N-(5-(3-(7-(3-((2-(dimethylamino)ethyl)amino)-5-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [1426];2-(dimethylamino)-N-(5-(3-(7-(3-(2-(dimethylamino)ethoxy)-5-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [1427];2-(dimethylamino)-N-(5-(3-(7-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [1428];2-(dimethylamino)-N-(5-(3-(7-(3-fluoro-5-hydroxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [1429];2-(dimethylamino)-N-(5-(3-(7-(3-fluoro-5-methoxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)acetamide [1430];4-(2-(5-(pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)morpholine [1431];3-(7-(4,4-difluoropiperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [1432];3-(7-(1-methylpiperidin-4-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [1433];4-(2-(5-(5-fluoropyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)morpholine [1434];5-(5-fluoropyridin-3-yl)-3-(7-(4-methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1435];3-(7-(4,4-difluoropiperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(5-fluoropyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [1436];5-(5-fluoropyridin-3-yl)-3-(7-(1-methylpiperidin-4-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1437];5-(5-fluoropyridin-3-yl)-3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolin[3,4-b]pyridine [1438];4-(2-(5-(1-cyclopropyl-1H-pyrazol-4-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)morpholine [1442];5-(1-cyclopropyl-1H-pyrazol-4-yl)-3-(7-(4-methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1443];5-(1-cyclopropyl-1H-pyrazol-4-yl)-3-(7-(4,4-difluoropiperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1444];5-(1-cyclopropyl-1H-pyrazol-4-yl)-3-(7-(1-methylpiperidin-4-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1445]; and5-(1-cyclopropyl-1H-pyrazol-4-yl)-3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1446]; or a pharmaceutically acceptable salt thereof.

25. The method of claim 11, wherein the compound of Formula I is selected from the group consisting of: N-(5-(3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)propionamide [133];3-methyl-N-(5-(3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)butanamide [134];5-(4-methylpyridin-3-yl)-3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [137];N-(5-(3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)pivalamide [140];N-(5-(3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)isobutyramide [141];3,3-dimethyl-N-(5-(3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)butanamide [148];N-(5-(3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)butyramide [149];N-(5-(3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)pentanamide [150];N-(5-(3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclopropanecarboxamide [151];N-(5-(3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclobutanecarboxamide [152];N-(5-(3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclopentanecarboxamide [153];N-(5-(3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclohexanecarboxamide [154];3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyrimidin-5-yl)-1H-pyrazolo[3,4-b]pyridine [158];N-(5-(3-(7-(4-methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)propionamide [184];3-methyl-N-(5-(3-(7-(4-methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)butanamide [185];N-(5-(3-(7-(4-methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)pivalamide [191];N-(5-(3-(7-(4-methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)isobutyramide [192];3,3-dimethyl-N-(5-(3-(7-(4-methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)butanamide [199];N-(5-(3-(7-(4-methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)butyramide [200];N-(5-(3-(7-(4-methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)pentanamide [201];N-(5-(3-(7-(4-methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclopropanecarboxamide [202];N-(5-(3-(7-(4-methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclobutanecarboxamide [203];N-(5-(3-(7-(4-methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclopentanecarboxamide [204];N-(5-(3-(7-(4-methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclohexanecarboxamide [205];3-(7-(4-methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyrimidin-5-yl)-1H-pyrazolo[3,4-b]pyridine [209];N-(5-(3-(7-(thiophen-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)propionamide [233];3-methyl-N-(5-(3-(7-(thiophen-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)butanamide [234];5-(4-methylpyridin-3-yl)-3-(7-(thiophen-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [237];N-(5-(3-(7-(thiophen-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)pivalamide [240];N-(5-(3-(7-(thiophen-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)isobutyramide [241];3,3-dimethyl-N-(5-(3-(7-(thiophen-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)butanamide [248];N-(5-(3-(7-(thiophen-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)butyramide [249];N-(5-(3-(7-(thiophen-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)pentanamide [250];N-(5-(3-(7-(thiophen-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclopropanecarboxamide [251];N-(5-(3-(7-(thiophen-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclobutanecarboxamide [252];N-(5-(3-(7-(thiophen-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclopentanecarboxamide [253];N-(5-(3-(7-(thiophen-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclohexanecarboxamide [254];5-(pyrimidin-5-yl)-3-(7-(thiophen-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [258];N-(5-(3-(7-(furan-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)propionamide [259];N-(5-(3-(7-(furan-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-3-methylbutanamide [260];3-(7-(furan-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(4-methylpyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [263];N-(5-(3-(7-(furan-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)pivalamide [266];N-(5-(3-(7-(furan-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)isobutyramide [267];N-(5-(3-(7-(furan-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-3,3-dimethylbutanamide [274];N-(5-(3-(7-(furan-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)butyramide [275];N-(5-(3-(7-(furan-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)pentanamide [276];N-(5-(3-(7-(furan-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclopropanecarboxamide [277];N-(5-(3-(7-(furan-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclobutanecarboxamide [278];N-(5-(3-(7-(furan-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclopentanecarboxamide [279];N-(5-(3-(7-(furan-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclohexanecarboxamide [280];3-(7-(furan-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyrimidin-5-yl)-1H-pyrazolo[3,4-b]pyridine 12841;N-(5-(3-(7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)propionamide [285];3-methyl-N-(5-(3-(7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)butanamide [286];S -(4-methylpyridin-3-yl)-3-(7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [289];N-(5-(3-(7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)pivalamide [292];N-(5-(3-(7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)isobutyramide [293];3,3-dimethyl-N-(5-(3-(7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)butanamide [300];N-(5-(3-(7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)butyramide [301];N-(5-(3-(7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)pentanamide [302];N-(5-(3-(7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclopropanecarboxamide [303];N-(5-(3-(7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclobutanecarboxamide [304];N-(5-(3-(7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclopentanecarboxamide [305];N-(5-(3-(7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclohexanecarboxamide [306];5-(pyrimidin-5-yl)-3-(7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [310];N-(5-(3-(7-(5-fluorothiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)propionamide [311];N-(5-(3-(7-(5-fluorothiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-3-methylbutanamide [312];3-(7-(5-fluorothiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(4-methylpyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [315];N-(5-(3-(7-(5-fluorothiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)pivalamide [318];N-(5-(3-(7-(5-fluorothiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)isobutyramide [319];N-(5-(3-(7-(5-fluorothiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-3,3-dimethylbutanamide [326];N-(5-(3-(7-(5-fluorothiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)butyramide [327];N-(5-(3-(7-(5-fluorothiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)pentanamide [328];N-(5-(3-(7-(5-fluorothiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclopropanecarboxamide [329];N-(5-(3-(7-(5-fluorothiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclobutanecarboxamide [330];N-(5-(3-(7-(5-fluorothiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclopentanecarboxamide [331];N-(5-(3-(7-(5-fluorothiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclohexanecarboxamide [332];3-(7-(5-fluorothiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyrimidin-5-yl)-1H-pyrazolo[3,4-b]pyridine [336];N-(5-(3-(7-(5-methylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)propionamide [337];3-methyl-N-(5-(3-(7-(5-methyhthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)butanamide [338];5-(4-methylpyridin-3-yl)-3-(7-(5-methylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [341];N-(5-(3-(7-(5-methylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)pivalamide [344];N-(5-(3-(7-(5-methylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)isobutyramide [345];3,3-dimethyl-N-(5-(3-(7-(5-methylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)butanamide [352];N-(5-(3-(7-(5-methylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)butyramide [353];N-(5-(3-(7-(5-methylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)pentanamide [354];N-(5-(3-(7-(5-methylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclopropanecarboxamide [355];N-(5-(3-(7-(5-methylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclobutanecarboxamide [356];N-(5-(3-(7-(5-methylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclopentanecarboxamide [357];N-(5-(3-(7-(5-methylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclohexanecarboxamide [358];3-(7-(5-methylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyrimidin-5-yl)-1H-pyrazolo[3,4-b]pyridine [362];N-(5-(3-(7-(5-acetylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)propionamide [363];N-(5-(3-(7-(5-acetylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-3-methylbutanamide [364];1-(5-(2-(5-(4-methylpyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)thiophen-2-yl)ethanone [367];N-(5-(3-(7-(5-acetylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)pivalamide [370];N-(5-(3-(7-(5-acetylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)isobutyramide [371];N-(5-(3-(7-(5-acetylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-3,3-dimethylbutanamide [378];N-(5-(3-(7-(5-acetylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)butyramide [379];N-(5-(3-(7-(5-acetylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)pentanamide [380];N-(5-(3-(7-(5-acetylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclopropanecarboxamide [381];N-(5-(3-(7-(5-acetylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclobutanecarboxamide [382];N-(5-(3-(7-(5-acetylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-indazol-5-yl)pyridin-3-yl)cyclopentanecarboxamide [383];N-(5-(3-(7-(5-acetylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclohexanecarboxamide [384];1-(5-(2-(5-(pyrimidin-5-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)thiophen-2-yl)ethanone [388];5-(1-methyl-1H-pyrazol-4-yl)-3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1020];5-(1,2-dimethyl-1H-imidazol-5-yl)-3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1021];5-(1-methyl-1H-pyrazol-4-yl)-3-(7-(4-methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1056];5-(1,2-dimethyl-1H-imidazol-5-yl)-3-(7-(4-methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1057];5-(1-methyl-1H-pyrazol-4-yl)-3-(7-(thiophen-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1092];5-(1,2-dimethyl-1H-imidazol-5-yl)-3-(7-(thiophen-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1093];3-(7-(furan-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(1-methyl-1H-pyrazol-4-yl)-1H-pyrazolo[3,4-b]pyridine [1110];5-(1,2-dimethyl-1H-imidazol-5-yl)-3-(7-(furan-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo [3,4-b]pyridine [1111];5-(1-methyl-1H-pyrazol-4-yl)-3-(7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1128];5-(1,2-dimethyl-1H-imidazol-5-yl)-3-(7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1129];3-(7-(5-fluorothiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(1-methyl-1H-pyrazol-4-yl)-1H-pyrazolo[3,4-b]pyridine [1146];5-(1,2-dimethyl-1H-imidazol-5-yl)-3-(7-(5-fluorothiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1147];5-(1-methyl-1H-pyrazol-4-yl)-3-(7-(5-methylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1164];5-(1,2-dimethyl-1H-imidazol-5-yl)-3-(7-(5-methylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1165];1-(5-(2-(5-(1-methyl-1H-pyrazol-4-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)thiophen-2-yl)ethan-1-one [1182];1-(5-(2-(5-(1,2-dimethyl-1H-imidazol-5-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)thiophen-2-yl)ethan-1-one [1183];4-(2-(5-(1-methyl-1H-pyrazol-4-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)morpholine [1439];3-(7-(4,4-difluoropiperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(1-methyl-1H-pyrazol-4-yl)-1H-pyrazolo[3,4-b]pyridine [1440]; and5-(1-methyl-1H-pyrazol-4-yl)-3-(7-(1-methylpiperidin-4-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1441]; or a pharmaceutically acceptable salt thereof.

26. A compound or pharmaceutically acceptable salt thereof having the structure of: N-((5-(3-(7-(3-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl) pyridin-3-yl)methyl)ethanamine [4];3-(7-(3-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyrimidin-5-yl)-1H-pyrazolo[3,4-b]pyridine [22];N-((5-(3-(7-(4-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methyl)ethanamine [26];3-(7-(4-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyrimidin-5-yl)-1H-pyrazolo[3,4-b]pyridine [44];N-((5-(3-(7-(2-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methyl)ethanamine [48];3-(7-(2-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyrimidin-5-yl)-1H-pyrazolo[3,4-b]pyridine [66];N-((5-(3-(7-(pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methyl)ethanamine [70];3-(7-(pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyrimidin-5-yl)-1H-pyrazolo[3,4-b]pyridine [88];N-((5-(3-(7-(pyridin-4-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methyl)ethanamine [92];3-(7-(pyridin-4-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyrimidin-5-yl)-1H-pyrazolo[3,4-b]pyridine [110];N-((5-(3-(7-(pyridin-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methyl)ethanamine [114];3-(7-(pyridin-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyrimidin-5-yl)-1H-pyrazolo[3,4-b]pyridine [132];N-((5-(3-(7-(piperidin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrarazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methyl)ethanamine [138];N-(5-(3-(7-(4-methyl-1H-imidazol-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)propionamide [159];3-methyl-N-(5-(3-(7-(4-methyl-1H-imidazol-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)butanamide [160];N-(5-(3-(7-(4-methyl-1H-imidazol-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)pivalamide [165];N-(5-(3-(7-(4-methyl-1H-imidazol-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)isobutyramide [166];3,3-dimethyl-N-(5-(3-(7-(4-methyl-1H-imidazol-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)butanamide [173];N-(5-(3-(7-(4-methyl-1H-imidazol-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)butyramide [174];N-(5-(3-(7-(4-methyl-1H-imidazol-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)pentanamide [175];N-(5-(3-(7-(4-methyl-1H-imidazol-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclopropanecarboxamide [176];N-(5-(3-(7-(4-methyl-1H-imidazol-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclobutanecarboxamide [177];N-(5-(3-(7-(4-methyl-1H-imidazol-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclopentanecarboxamide [178];N-(5-(3-(7-(4-methyl-1H-imidazol-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclohexanecarboxamide [179];3-(7-(4-methyl-1H-imidazol-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyrimidin-5-yl)-1H-pyrazolo[3,4-b]pyridine [183];N-((5-(3-(7-(4-methylpiperazin-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methyl)ethanamine [189];N-((5-(3-(3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methyl)ethanamine [213];N-(5-(3-(3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclopropanecarboxamide [225];N-(5-(3-(3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclobutanecarboxamide [226];N-(5-(3-(3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclopentanecarboxamide [227];N-(5-(3-(3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclohexanecarboxamide [228];3-(3H-imidazo[4,5-b]pyridin-2-yl)-5-(pyrimidin-5-yl)-1H-pyrazolo[3,4-b]pyridine [232];N-((5-(3-(7-(thiophen-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methyl)ethanamine [238];N-((5-(3-(7-(furan-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methyl)ethanamine [264];N-((5-(3-(7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methyl)ethanamine [290];N-((5-(3-(7-(5-fluorothiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methyl)ethanamine [316];N-((5-(3-(7-(5-methylthiophen-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methyl)ethanamine [342];1-(5-(2-(5-(5-((ethylamino)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo [4,5-b]pyridin-7-yl)thiophen-2-yl)ethanone [368];N-(5-(3-(7-(3-fluoro-5-(methylsulfonamidomethyl)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)propionamide [389];N-(5-(3-(7-(3-fluoro-5-(methylsulfonamidomethyl)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-3-methylbutanamide [390];N-(3-fluoro-5-(2-(5-(4-methylpyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)benzyl)methane sulfonamide [393];N-(3-(2-(5-(5-((ethylamino)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo [4,5-b]pyridin-7-yl)-5-fluorobenzyl)methanesulfonamide [394];N-(5-(3-(7-(3-fluoro-5-(methylsulfonamidomethyl)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)pivalamide [396];N-(5-(3-(7-(3-fluoro-5-(methylsulfonamidomethyl)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)isobutyramide [397];N-(5-(3-(7-(3-fluoro-5-(methylsulfonamidomethyl)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-3,3-dimethylbutanamide [404];N-(5-(3-(7-(3-fluoro-5-(methylsulfonamidomethyl)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)butyramide [405];N-(5-(3-(7-(3-fluoro-5-(methylsulfonamidomethyl)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)pentanamide [406];N-(5-(3-(7-(3-fluoro-5-(methylsulfonamidomethyl)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclopropanecarboxamide [407];N-(5-(3-(7-(3-fluoro-5-(methylsulfonamidomethyl)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclobutanecarboxamide [408];N-(5-(3-(7-(3-fluoro-5-(methylsulfonamidomethyl)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclopentanecarboxamide [409];N-(5-(3-(7-(3-fluoro-5-(methylsulfonamidomethyl)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclohexanecarboxamide [410];N-(3-fluoro-5-(2-(5-(pyrimidin-5-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)benzyl)methanesulfonamide [414];N-(5-(3-(7-(3-((2-(dimethylamino)ethyl)amino)-5-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)propionamide [415];N-(5-(3-(7-(3-((2-(dimethylamino)ethyl)amino)-5-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-3-methylbutanamide [416];N1-(3-fluoro-5-(2-(5-(4-methylpyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)phenyl)-N2,N2-dimethylethane-1,2-diamine [419];N1-(3-(2-(5-(5-((ethylamino)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)-5-fluorophenyl)-N2,N2-dimethylethane-1,2-diamine [420];N-(5-(3-(7-(3-((2-(dimethylamino)ethyl)amino)-5-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)pivalamide [422];N-(5-(3-(7-(3-((2-(dimethylamino)ethyl)amino)-5-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)isobutyramide [423];N-(5-(3-(7-(3-((2-(dimethylamino)ethyl)amino)-5-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-3,3-dimethylbutanamide [430];N-(5-(3-(7-(3-((2-(dimethylamino)ethyl)amino)-5-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)butyramide [431];N-(5-(3-(7-(3-((2-(dimethylamino)ethyl)amino)-5-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)pentanamide [432];N-(5-(3-(7-(3-((2-(dimethylamino)ethyl)amino)-5-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclopropanecarboxamide [433];N-(5-(3-(7-(3-((2-(dimethylamino)ethyl)amino)-5-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclobutanecarboxamide [434];N-(5-(3-(7-(3-((2-(dimethylamino)ethyl)amino)-5-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclopentanecarboxamide [435];N-(5-(3-(7-(3-((2-(dimethylamino)ethyl)amino)-5-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclohexanecarboxamide [436];N1-(3-fluoro-5-(2-(5-(pyrimidin-5-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)phenyl)-N2,N2-dimethylethane-1,2-diamine [440]3-(7-(3-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(1-methyl-1H-pyrazol-4-yl)-1H-pyrazolo[3,4-b]pyridine [912];5-(1,2-dimethyl-1H-imidazol-5-yl)-3-(7-(3-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [913];3-(7-(4-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(1-methyl-1H-pyrazol-4-yl)-1H-pyrazolo[3,4-b]pyridine [930];5-(1,2-dimethyl-1H-imidazol-5-yl)-3-(7-(4-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [931];3-(7-(2-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(1-methyl-1H-pyrazol-4-yl)-1H-pyrazolo[3,4-b]pyridine [948];5-(1,2-dimethyl-1H-imidazol-5-yl)-3-(7-(2-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [949];5-(1-methyl-1H-pyrazol-4-yl)-3-(7-(pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [966];5-(1,2-dimethyl-1H-imidazol-5-yl)-3-(7-(pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [967];5-(1-methyl-1H-pyrazol-4-yl)-3-(7-(pyridin-4-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazol[3,4-b]pyridine [984];5-(1,2-dimethyl-1H-imidazol-5-yl)-3-(7-(pyridin-4-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [985];5-(1-methyl-1H-pyrazol-4-yl)-3-(7-(pyridin-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazol[3,4-b]pyridine [1002];5-(1,2-dimethyl-1H-imidazol-5-yl)-3-(7-(pyridin-2-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1003];3-(7-(4-methyl-1H-imidazol-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(1-methyl-1H-pyrazol-4-yl)-1H-pyrazolo[3,4-b]pyridine [1038];5-(1,2-dimethyl-1H-imidazol-5-yl)-3-(7-(4-methyl-1H-imidazol-1-yl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1039];3-(3H-imidazo[4,5-b]pyridin-2-yl)-5-(1-methyl-1H-pyrazol-4-yl)-1H-pyrazolo[3,4-b]pyridine [1074];5-(1,2-dimethyl-1H-imidazol-5-yl)-3-(3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1075];N-(3-fluoro-5-(2-(5-(1-methyl-1H-pyrazol-4-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)benzyl)methanesulfonamide [1200];N-(3-(2-(5-(1,2-dimethyl-1H-imidazol-5-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)-5-fluorobenzyl)methanesulfonamide [1201];N1-(3-fluoro-5-(2-(5-(1-methyl-1H-pyrazol-4-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)phenyl)-N2,N2-dimethylethane-1,2-diamine [1218];N1-(3-(2-(5-(1,2-dimethyl-1H-imidazol-5-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)-5-fluorophenyl)-N2,N2-dimethylethane-1,2-diamine [1219];N-(5-(3-(7-(3-(2-(dimethylamino)ethoxy)-5-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)propionamide [1233];N-(5-(3-(7-(3-(2-(dimethylamino)ethoxy)-5-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-3-methylbutanamide [1234];2-(3-fluoro-5-(2-(5-(4-methylpyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)phenoxy)-N,N-dimethylethan-1-amine [1237];2-(3-(2-(5-(5-((ethylamino)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)-5-fluorophenoxy)-N,N-dimethylethan-1-amine [1238];N-(5-(3-(7-(3-(2-(dimethylamino)ethoxy)-5-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)pivalamide [1240];N-(5-(3-(7-(3-(2-(dimethylamino)ethoxy)-5-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)isobutyramide [1241];N-(5-(3-(7-(3-(2-(dimethylamino)ethoxy)-5-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-3,3-dimethylbutanamide [1248];N-(5-(3-(7-(3-(2-(dimethylamino)ethoxy)-5-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)butyramide [1249];N-(5-(3-(7-(3-(2-(dimethylamino)ethoxy)-5-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)pentanamide [1250];N-(5-(3-(7-(3-(2-(dimethylamino)ethoxy)-5-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclopropanecarboxamide [1251];N-(5-(3-(7-(3-(2-(dimethylamino)ethoxy)-5-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclobutanecarboxamide [1252];N-(5-(3-(7-(3-(2-(dimethylamino)ethoxy)-5-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclopentanecarboxamide [1253];N-(5-(3-(7-(3-(2-(dimethylamino)ethoxy)-5-fluorophenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclohexanecarboxamide [1254];2-(3-fluoro-5-(2-(5-(1-methyl-1H-pyrazol-4-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)phenoxy)-N,N-dimethylethan-1-amine [1263];2-(3-(2-(5-(1,2-dimethyl-1H-imidazol-5-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)-5-fluorophenoxy)-N,N-dimethylethan-1-amine [1264];N-(5-(3-(7-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)propionamide [1277];N-(5-(3-(7-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-3-methylbutanamide [1278];3-(7-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(4-methylpyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [1281];N-((5-(3-(7-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methyl)ethanamine [1282];N-(5-(3-(7-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)pivalamide [1284];N-(5-(3-(7-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)isobutyramide [1285];N-(5-(3-(7-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-3,3-dimethylbutanamide [1292];N-(5-(3-(7-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)butyramide [1293];N-(5-(3-(7-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)pentanamide [1294];N-(5-(3-(7-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclopropanecarboxamide [1295];N-(5-(3-(7-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclobutanecarboxamide [1296];N-(5-(3-(7-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclopentanecarboxamide [1297];N-(5-(3-(7-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclohexanecarboxamide [1298];3-(7-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(1-methyl-1H-pyrazol-4-yl)-1H-pyrazolo[3,4-b]pyridine [1307];5-(1,2-dimethyl-1H-imidazol-5-yl)-3-(7-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1308];N-(5-(3-(7-(3-fluoro-5-hydroxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)propionamide [1321];N-(5-(3-(7-(3-fluoro-5-hydroxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-3-methylbutanamide [1322];3-fluoro-5-(2-(5-(4-methylpyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)phenol [1325];3-(2-(5-(5-((ethylamino)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)-5-fluorophenol [1326];N-(5-(3-(7-(3-fluoro-5-hydroxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)pivalamide [1328];N-(5-(3-(7-(3-fluoro-5-hydroxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)isobutyramide [1329];N-(5-(3-(7-(3-fluoro-5-hydroxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-3,3-dimethylbutanamide [1336];N-(5-(3-(7-(3-fluoro-5-hydroxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)butyramide [1337];N-(5-(3-(7-(3-fluoro-5-hydroxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)pentanamide [1338];N-(5-(3-(7-(3-fluoro-5-hydroxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclopropanecarboxamide [1339];N-(5-(3-(7-(3-fluoro-5-hydroxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclobutanecarboxamide [1340];N-(5-(3-(7-(3-fluoro-5-hydroxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclopentanecarboxamide [1341];N-(5-(3-(7-(3-fluoro-5-hydroxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclohexanecarboxamide [1342];3-fluoro-5-(2-(5-(1-methyl-1H-pyrazol-4-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)phenol [1351];3-(2-(5-(1,2-dimethyl-1H-imidazol-5-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl)-3H-imidazo[4,5-b]pyridin-7-yl)-5-fluorophenol [1352];N-(5-(3-(7-(3-fluoro-5-methoxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)propionamide [1365];N-(5-(3-(7-(3-fluoro-5-methoxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-3-methylbutanamide [1366];3-(7-(3-fluoro-5-methoxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(4-methylpyridin-3-yl)-1H-pyrazolo[3,4-b]pyridine [1369];N-((5-(3-(7-(3-fluoro-5-methoxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)methyl)ethanamine [1370];N-(5-(3-(7-(3-fluoro-5-methoxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)pivalamide [1372];N-(5-(3-(7-(3-fluoro-5-methoxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)isobutyramide [1373];N-(5-(3-(7-(3-fluoro-5-methoxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)-3,3-dimethylbutanamide [1380];N-(5-(3-(7-(3-fluoro-5-methoxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)butyramide [1381];N-(5-(3-(7-(3-fluoro-5-methoxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)pentanamide [1382];N-(5-(3-(7-(3-fluoro-5-methoxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclopropanecarboxamide [1383];N-(5-(3-(7-(3-fluoro-5-methoxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclobutanecarboxamide [1384];N-(5-(3-(7-(3-fluoro-5-methoxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclopentanecarboxamide [1385];N-(5-(3-(7-(3-fluoro-5-methoxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridin-5-yl)pyridin-3-yl)cyclohexanecarboxamide [1386];3-(7-(3-fluoro-5-methoxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-5-(1-methyl-1H-pyrazol-4-yl)-1H-pyrazolo[3,4-b]pyridine [1395]; and5-(1,2-dimethyl-1H-imidazol-5-yl)-3-(7-(3-fluoro-5-methoxyphenyl)-3H-imidazo[4,5-b]pyridin-2-yl)-1H-pyrazolo[3,4-b]pyridine [1396]; or a pharmaceutically acceptable salt thereof.

27. The method of claim 1, wherein the fibrotic disorder is a skin fibrosis.

28. The method of claim 1, wherein the fibrotic disorder is scleroderma.

29. The method of claim 1, wherein the fibrotic disorder is pulmonary fibrosis.

30. The method of claim 1, wherein the fibrotic disorder is renal fibrosis.

31. The method of claim 1, wherein the fibrotic disorder is idiopathic pulmonary fibrosis (IPF).

32. The method of claim 1, wherein the fibrotic disorder is cirrhosis of the liver.

33. The method of claim 1, wherein the fibrotic disorder is liver fibrosis.

34. The method of claim 1, wherein the subject is a human.