Patent Application
20060228965
The present Invention relates to selected 3-aryl-2-cyano-3-hydroxy-acrylic acid derivatives, the preparation of these compounds, and to the use of such compounds for the antimicrobial treatment of surfaces, as antimicrobial active substances against gram-positive and gram-negative bacteria, yeasts and fungi and also for the preservation of cosmetics, household products, textiles and plastics and for use as disinfectants.
The present Invention relates to 3-aryl-2-cyano-3-hydroxy-acrylic acid derivatives of formula
R1 is hydrogen, C1-C20alkyl; C1-C20alkoxy; CF3; C8-C10aryl; or a radical of formu—
R2 is hydrogen; or C1-C20alkyl.
C1-C20alkyl are straight-chain or branched alkyl radicals like methyl, ethyl, n-propyl, isopropyl, n-butyl, sec.butyl, tert.butyl, amyl, isoamyl or tert.amyl, hexyl, 2-ethylhexyl, heptyl, octyl, iso-octyl, nonyl, decyl, undecyl, dodecyl, tetradecyl, pentadecyl, hexadecyl, heptadecyl, octadecyl oder eicosyl.
C1-C20alkoxy are for example methoxy, ethoxy, n-propoxy, isopropoxy, n-butoxy, sek.butoxy, tert. butoxy, amyloxy, isoamyloxy oder tert.amyloxy, hexyloxy, heptyloxy, octyloxy, isooctyloxy, nonyloxy, decyloxy, undecyloxy, dodecyloxy, tetradecyloxy, pentadecyloxy, hexadecyloxy, heptadecyloxy oder octadecyloxy.
C8-C10aryl is for example naphthyl and preferably phenyl.
In accordance with the present invention, preference is given to compounds of formula (1a), wherein
R1 is C8-C10aryl, and most preferably phenyl.
Preference is given to compounds of formula (1a), (1b) or (1c), wherein R2 Is C1-C20-Alkyl.
Very special preference Is given to compounds of formulae
wherein in the formulas (2) to (5)
R2 Is C1-C20alkyl.
The preparation of the 3-aryl-2-cyano-3-hydroxy-acrylic acid derivatives can be carried out according to known methods.
For the preparation of the acrylic acid derivatives of the general formula (1a), (1b) or (1c) of the corresponding cyanoacetates of the formula (1d) are condensed with carbon acid chlorides of the formula (1c) at temperatures from −100° C. to +100° C., preferably at −80° C., in a suitable solvent, like diethylether or tetrahydrofuran in the presence of a base like metal alcoholates, metal silacides or metal amides, preferably lithium diisopropylamide according to the following reaction scheme:
R1 and R2 are defined as given in formula (1a), (1b) or (1c).
The process for the preparation of the 3-aryl-2-cyano-3-hydroxy-acrylic acid derivates is a further object of the present invention.
The 3-aryl-2-cyano-3-hydroxy-acrylic acid derivatives of the present invention exhibit pronounced antimicrobial action, especially against pathogenic gram-positive and gram-negative bacteria and against bacteria of the skin flora, and also against yeasts and moulds. They are accordingly suitable especially for disinfection, deodorisation, and for general and antimicrobial treatment of the skin and mucosa and of integumentary appendages (hair), more especially for the disinfection of hands and wounds.
They are accordingly suitable as antimicrobial active substances and preservatives in personal care preparations such as, for example, shampoos, bath additives, haircare preparations, liquid and solid soaps (based on synthetic surfactants and salts of saturated and/or unsaturated fatty acids), lotions and creams, deodorants, other aqueous or alcoholic solutions, e.g. cleansing solutions for the skin, moist cleaning cloths, oils or powders.
The present invention accordingly relates also to a personal care preparation comprising at least one compound of formula (1a), (1b) or (1c) and cosmetically tolerable carriers or adjuvants.
The personal care preparation according to the present invention contains from 0.01 to 15% by weight, preferably from 0.1 to 10% by weight, based on the total weight of the composition, of a compound of formula (1a), (1b) or (1c), and cosmetically tolerable adjuvants.
Depending upon the form of the personal care preparation, it comprises, in addition to the 3-aryl-2-cyano-3-hydroxy-acrylic acid derivatives of formula (1a), (1b) or (1c), further constituents such as, for example, sequestering agents, colorants, perfume oils, thickeners or solidifiers (consistency regulators), emollients, UV-absorbers, skin protective agents, antioxidants, additives that Improve the mechanical properties, such as dicarboxylic adds and/or aluminum, zinc, calcium or magnesium salts of C14-C22fatty acids, and, optionally, preservatives.
The personal care preparation according to the present invention may be In the form of a water-in-oil or oil-In-water emulsion, an alcoholic or alcohol-containing formulation, a vesicular dispersion of an Ionic or non-ionic amphiphilic lipid, a gel, a solid stick or an aerosol formulation.
As a water-in-oil or oil-In-water emulsion, the cosmetically tolerable adjuvant contains preferably from 5 to 50% of an oil phase, from 5 to 20% of an emulsifier and from 30 to 90% water. The oil phase may comprise any oil suitable for cosmetic formulations such as, for example, one or more hydrocarbon oils, a wax, a natural oil, a silicone oil, a fatty acid ester or a fatty alcohol. Preferred mono- or poly-ols are ethanol, isopropanol, propylene glycol, hexylene glycol, glycerol and sorbitol.
Cosmetic formulations according to the present invention are used In various fields, especially for example In the following preparations:
An antimicrobial soap has, for example, the following composition:
0.01 to 5% by weight of a compound of formula (1a), (1b) or (1c),
0.3 to 1% by weight titanium dioxide,
1 to 10% by weight stearic acid,
soap base ad 100%, e.g. a sodium salt of tallow fatty acid or coconut fatty acid, or glycerol.
A shampoo has, for example, the following composition:
0.01 to 5% by weight of a compound of formula (1a), (1b) or (1c),
12.0% by weight sodium laureth-2-aulfate,
4.0% by weight cocamidopropyl betalne,
3.0% by weight NaCl and
water ad 100%.
A deodorant has, for example, the following composition:
0.01 to 5% by weight of a compound of formula (1a), (1b) or (1c),
60% by weight ethanol,
0.3% by weight perfume oil, and
water ad 100%.
The present Invention relates also to an oral composition containing from 0.01 to 15% by weight, based on the total weight of the composition, of a compound of formula (1a), (1b) or (1c), and orally tolerable adjuvants.
Example of an oral composition:
10% by weight sorbitol,
10% by weight glycerol,
15% by weight ethanol,
15% by weight propylene glycol,
0.5% by weight sodium lauryl sulfate,
0.25% by weight sodium methylcocyl taurate,
0.25% by weight polyoxypropylenelpolyoxyethylene block copolymer,
0.10% by weight peppermint flavouring,
0.1 to 0.5% by weight of a compound of formula (1a), (1b) or (1c), and
48.6% by weight water.
The oral composition according to the present invention may be, for example, in the form of a gel, a paste, a cream or an aqueous preparation (mouthwash).
The oral composition according to the present Invention may also comprise compounds that release fluoride ions which are effective against the formation of caries, for example inorganic fluoride salts, e.g. sodium, potassium, ammonium or calcium fluoride, or organic fluoride salts, e.g. amine fluorides, which are known under the trade name Olafluor.
3-aryl-2-cyano-3-hydroxy-acrylic acid derivatives of formulae (1a), (1b) or (1c) according to the present invention are also suitable for treating, especially preserving, textile fibre materials. Such materials are undyed and dyed or printed fibre materials, for example made of silk, wool, polyamide or polyurethanes, and especially cellulosic fibre materials of all kinds. Such fibre materials are, for example, natural cellulose fibres, such as cotton, linen, jute and hemp, as well as cellulose and regenerated cellulose. Preferred suitable textile fibre materials are made of cotton.
3-aryl-2-cyano-3-hydroxy-acrylic acid derivatives according to the present invention am suitable also for treating, especially Imparting antimicrobial properties to or preserving, plastics such as, for example, polyethylene, polypropylene, polyurethane, polyester, polyamide, polycarbonate, latex etc,. Fields of use therefor are, for example, floor coverings, plastics coatings, plastics containers and packaging materials; kitchen and bathroom utensils (e.g. brushes, shower curtains, sponges, bathmats), latex, filter materials (air and water filters), plastics articles used in the field of medicine such as, for example, dressing materials, syringes, catheters etc., so-called “medical devices”, gloves and mattresses.
Paper, for example papers used for hygiene purposes, may also be provided with antimicrobial properties using the 3-aryl-2-cyano-3-hydroxy-acrylic acid derivatives of formulae (1a), (1b) or (1c) according to the present invention.
It is also possible for nonwovens such as, for example, nappies/diapers, sanitary towels, panty liners, and cloths for hygiene and household uses, to be provided with antimicrobial properties in accordance with the present invention.
The 3-aryl-2-cyano-3-hydroxy-acrylic acid derivatives of formulae (1a), (1b) or (1c) am also used in washing and cleaning formulations such as, for example, liquid or powder washing agents or softeners.
The 3-aryl-2 cyano-3-hydroxy-acrylic add derivatives of formulae (1a), (1b) or (1c) can also be used especially In household and general-purpose cleaners for cleaning and disinfecting hard surfaces.
A cleaning preparation has, for example, the following composition:
0.01 to 5% of a compound of formula (1a), (1b) or (1c),
3.0% octyl alcohol 4EO,
1.3% fatty alcohol C8-C10polyglucoside,
3.0% isopropanol, and
water ad 100%.
In addition to preserving cosmetic and household products, the preservation of technical products, the provision of technical products with antimicrobial properties and use as a biocide in technical processes are also possible, for example In paper treatment, especially in paper treatment liquors, printing thickeners of starch or cellulose derivatives, surface-coatings and paints.
The 3-aryl-2-cyano-3-hydroxy-acrylic acid derivatives of formulae (1a), (1b) or (1c) are also suitable for the antimicrobial treatment of wood and for the antimicrobial treatment of leather, the preserving of leather and the provision of leather with antimicrobial properties.
The compounds according to the present invention are also suitable for the protection of cosmetic products and household products from microbial damage.
Furthermore, the compounds of the present invention are able to penetrate biofilms formed on animated and inanimated surfaces. They can also, if necessary, prevent the adhesion of bacteria on surfaces and the further forming of the biofilmes. They are able to detach the biofilm and/or to inhibit the groth of the biofilm forming microorganisms in the biological matrix or to kill them.
Generally, biofilms are aggregates of animated or inanimated microorganisms, preferably bacteria, togehter with their intermediate catabolic products in form of extracellular polymer substances (EPS-matrix), like polysaccharides. The efficiacy of antimicrobial actives, which normally show a distinct growth-inhibion or killing activity against plankton cells, may strongly be reduced for microorganisms, which are organized In biofilms, i.e. because of insufficient penetration of the active into the biomatrix.
The compounds of the present Invention are therefore effective actives against biofilms which are formed on the surface of teeth and oral mucosa, which are participated in the formation of degenerative an diseases in the oral region, like caries or periodontosis.
The following Examples illustrate, but do not limit, the present invention.
A 1.6 m solution of 1 mmol n-BuLi In hexane Is added to a solution of 1 mmol diisopropylamine in THFabs (6 ml/ml diisopropylamine) at −10° C. The mixture Is stirred for 15 min. and than cooled down to −78° C.
After that the solution of 0.5 mmol of the corresponding cyano acetate is added In THF (1 ml/g cyanoacetate) at this temperature initially, additionally stirred for 15 min and than the solution of 0.5 mmol of the acid chloride is added in THF (2 ml/g acid chloride).
After stirring for 45 min at −78° C. the reaction mixture is quenched at this temperature with 15% HCl und warmed up to room temperature over night.
For working up the reaction mass is extracted with acetic acid ester, the organic phase is washed with water for several times, dried and purified and concentrated.
The product is isolated and purified via chromatographic methods on silica gel (acidic acid ester/hexane 10/1).
The following derivatives are obtained with this method:
A 1.6 m solution mmol of n-BuLi in hexane Is added at −10° C. to a solution of 1 mmol diisopropylamine In THFabs (6 ml/ml diisopropylamine). The mixture is stirred for 15 min. and than cooled down to −78° C.
After that the solution of 0.5 mmol of the corresponding cyano acetate is added in THF (1 ml/g cyanoacetate) at this temperature initially, additionally stirred for 15 min and than the solution of 0.5 mmol of the acid chloride is added in THF (2 ml/g acid chloride).
After stirring of 45 min at −78° C. the reaction mixture is quenched at this temperature with 15% HCl und warmed up to room temperature over night.
The working up for reconditioning, isolation and purification Is carried out according to example 1.
The following derivatives are obtained with this method:
To a solution of α-cyanoester (1 mmol), MgCl2 (1 mmol) in dry CH3CN (5 mL/g of aroyl chloride), maintained at 0° C. is added NEt3 (2 mmol) and the mixture is stirred for 5 min.
DMAP (0.5 mmol) is added and the mixture is stirred further for 15 min.
To this mixture is then added acid chloride (1 mmol), the reaction mass is stirred at 0° C. for 45 min and a further 1 h at RT.
The reaction mixture is quenched with 15% HCl at 0° C. and extracted with ethyl acetate.
The organic phase is washed with water, brine and dried over Na2SO4.
It is concentrated under reduced pressure and the crude is subjected to column chromatography over silica gel.
Further purification is achieved by a recolumn.
The yields for various β-aryl-α-cyano-β-ketoesters are given in Table 2. 1H-NMR and 13C-NMR data for these derivatives are given in Tables 3 and 4 respectively.
1H NMR spectral data
1H NMR data
13C spectral data
13C data
a) Automatic MIC-Screening according to the broth dilution method:
Test Principle:
8 μl of each dilution step is mixed with 192 μl of nutrient medium in micro titer plates. The plates are incubated at 37° C. for 24 hours and then the highest dilution (lowest concentration) of the test substance at which growth is just no longer discernible is determined (corresponds to the MIC).
b) Determination of MIC vs. different oral germs in the broth dilution method
Examples of Test
Test Principle:
Bacteria are taken from blood agar plates with cotton swabs and a suitable O.D. is adjusted In the corresponding medium (McFarland 0.5). This solution is used for F. nucleatum and P. nigrescens in undiluted state, for the other strains a dilution of 1:20 Is used. To each 2 ml of active ingredient containing solution 0.1 ml of the bacteria culture is added and incubated as described above.
The results are listed in Table 5.
c) Determination of MIC for an extended germ spectrum in the Agar incorporation test
Test Principle:
0.3 ml of each dilution step is mixed with 15 ml of nutrient medium while the latter is still liquid. After the nutrient medium has solidified, 10 μl of each of the following organism dilutions of the test strains in 0.85% NaCl solution are spotted onto the agar medium.
The plates are incubated during 24 hours at 37° C. and then the highest dilution (lowest concentration) of the test substance at which growth is just no longer discernible (corresponds to the MIC) is determined.
| Number | Date | Country | Kind |
|---|---|---|---|
| 03102324.5 | Jul 2003 | EP | regional |
| Filing Document | Filing Date | Country | Kind | 371c Date |
|---|---|---|---|---|
| PCT/EP04/51533 | 7/19/2004 | WO | 1/25/2006 |
