4-amino-2-(2-pyridyl)pyrimidines as microbicidal active substances

Abstract

There are described compounds of formula 1

Description

[0001] The present invention relates to substituted 4-amino-2-(2-pyridyl)pyrimidines, to the pre-paration of such compounds, and to their use for the antimicrobial treatment of surfaces, as antimicrobial active substances against gram-positive and gram-negative bacteria, yeasts and fungi and also in the preservation of cosmetics, household products, textiles and plastics and for use in disinfectants.

[0002] The substituted 4-amino-2-(2-pyridyl)pyrimidines according to the invention correspond to formula 2

[0003] wherein

[0004] R1 and R2 are each independently of the other hydrogen; unsubstituted or mono- or poly-halo-substituted C1-C20alkyl, C1-C20alkoxy, C2-C20alkenyl, C2-C20alkynyl, C3-C18cycloalkyl, C3-C7cycloalkyl-C1-C20alkyl; hydroxy; C1-C6alkoxy-C1-C20alkyl; carboxy; C1-C6alkyl-oxycarbonyl; cyano; mono- or di-C1-C20alkylamino; C1-C6alkylamino-C1-C20alkyl; halogen; phenyl; unsubstituted or C1-C5alkyl-, halo- or hydroxy-substituted phenyl-C1-C20alkyl, phenoxy or phenyl-C1-C20alkoxy; or R1 and R2 form a polymethylene chain of formula —(CH2)m— wherein m=2-12;

[0005] R3 is unsubstituted C7-C20alkyl; or amino-, hydroxy-, carboxy- or C1-C6alkyloxycarbonyl-substituted C2-C20alkyl, C8-C18cycloalkyl, C8-C20alkenyl, C8-C20alkynyl, C3-C7cycloalkyl-C8-C20alkyl, C1-C4alkoxy-C8-C20alkyl, R7R8N—C7-C20alkyl, phenyl, phenyl-C1-C4alkyl or phenyl-C1-C4alkoxy;

[0006] R4 is hydrogen; unsubstituted or C1-C5alkyl-, halo- or hydroxy-substituted C1-C20alkyl, C2-C20alkenyl, C2-C20alkynyl, C3-C20cycloalkyl, C3-C7cycloalkyl-C1-C20alkyl, C1-C20alkoxy-C1-C6alkyl or R7R8N—C1-C20alkyl, phenyl, phenyl-C1-C20alkyl or phenoxy-C1-C20alkyl;

[0007] R5 and R6 are each independently of the other hydrogen; C1-C20alkyl; C2-C20alkenyl; C2-C20-alkynyl; C3-C18cycloalkyl; C3-C7cycloalkyl-C1-C20alkyl; hydroxy; C2-C20alkoxy; C1-C6alkoxy-C1-C20alkyl; carboxy; C1-C6alkyloxycarbonyl; cyano; nitro; C1-C20alkylamino; C1-C20alkylaminoalkyl; C1-C20haloalkyl; C1-C20haloalkoxy; halogen; unsubstituted or C1-C5alkyl-, halo- or hydroxy-substituted phenyl, phenoxy or phenyl-C1-C20alkyl or phenyl-C1-C20alkoxy; or R5 and R6 together form a polymethylene chain of formula —(CH2)m— wherein m=2-12; and

[0008] R7 and R8 are each independently of the other hydrogen; C1-C20alkyl; C3-C20alkenyl; C3-C20-alkynyl; C3-C7cycloalkyl; C3-C20cycloalkyl-C1-C4alkyl; phenyl; or phenyl-C1-C4alkyl.

[0009] C1-C20Alkyl radicals are straight-chain or branched alkyl radicals, for example methyl, ethyl, n-propyl, isopropyl, n-butyl, sec-butyl, tert-butyl, amyl, isoamyl or tert-amyl, heptyl, octyl, isooctyl, nonyl, decyl, undecyl, dodecyl, tetradecyl, pentadecyl, hexadecyl, heptadecyl, octadecyl or eicosyl.

[0010] C3-C18Cycloalkyl denotes, for example, cyclopropyl, cyclobutyl, cyclopentyl, cycloheptyl, cyclooctyl, cyclononyl, cyclodecyl, cycloundecyl, cyclododecyl, cyclotetradecyl, cyclopenta-decyl, cyclohexadecyl, cycloheptadecyl, cyclooctadecyl or, especially, cyclohexyl.

[0011] Alkenyl includes, within the scope of the meanings given, inter alia, allyl, isopropenyl, 2-butenyl, 3-butenyl, isobutenyl, n-penta-2,4-dienyl, 3-methyl-but-2-enyl, n-oct-2-enyl, n-dodec-2-enyl, isododecenyl, n-dodec-2-enyl and n-octadec-4-enyl.

[0012] C1-C5Alkoxy radicals are straight-chain or branched radicals, for example methoxy, ethoxy, propoxy, butoxy or pentyloxy.

[0013] Alkynyl includes, for example, ethynyl, propargyl, 2-butynyl, 1-pentynyl and 2-pentynyl.

[0014] Preference is given to compounds of formula (1) wherein

[0015] R5 and R6 are each independently of the other hydrogen or C1-C20alkyl, very especially hydrogen.

[0016] R1 and R2 in formula (1) are, each independently of the other, preferably hydrogen, unsubstituted or mono- or poly-halo-substituted C1-C20alkyl; or C1-C5alkoxy-C1-C5alkyl; especially hydrogen; unsubstituted or mono- or poly-halo-substituted C1-C5alkyl; or C1-C3alkoxy-C1-C5alkyl; and more especially hydrogen, methyl, ethyl, isopropyl, tert-butyl, CF3 or the radical —(CH2)m—O—CH3 wherein m is from 1 to 4.

[0017] R3 in formula (1) is preferably unsubstituted or amino-substituted C7-C20alkyl, C3-C7cycloalkyl-C8-C20alkyl, C1-C4alkoxy-C7-C20alkyl, R7R8N—C7-C20alkyl, phenyl-C1-C4alkyl or phenyl-C1-C6alkoxy, especially unsubstituted or amino-substituted C7-C20alkyl or R7R8N—C7-C20alkyl.

[0018] Especially preferred compounds of formula (1) are those wherein

[0019] R1 and R2 are each independently of the other hydrogen, methyl, ethyl, isopropyl, tert-butyl, CF3 or the radical —(CH2)m—O—CH3;

[0020] R3 is unsubstituted or amino-substituted C7-C20alkyl or R7R8N—C7-C20alkyl;

[0021] R4 is hydrogen or C1-C20alkyl;

[0022] R5, R6, R7 and R8 are each independently of the others hydrogen or C1-C20alkyl; and

[0023] m is from 1 to 4,

[0024] especially those wherein

[0025] R5, R6, R7 and R8 are hydrogen.

[0026] Special preference is given to compounds of formulae 3

[0027] n is from 7 to 20; and

[0028] R1, R2, R3, R5 and R6 are as defined for formula (1).

[0029] All those compounds may also be present in the form of their acid addition salts, suitable acids being: HF, HCl, HBr, H2SO4, H3PO4, mono- and di-functional carboxylic acids, for example lactic acid, tartaric acid, acetic acid, maleic acid, fumaric acid, citric acid and salicylic acid, or sulfonic acid.

[0030] Table 1 below lists further 4-amino-2-(2-pyridyl)pyrimidines according to the invention by way of example:

TABLE 1
General formula
4
Compound
of
formula	R1	R2	R3
4	—CH3	—C2H5	—NH—(CH2)7—NH2
5	—CH3	—C2H5	—NH—(CH2)8—NH2
6	—CH3	—C2H5	—NH—(CH2)9—NH2
7	—CH3	—C2H5	—NH—(CH2)12—NH2
8	—CH3	—C2H5	—NH—(CH2)6—CH3
9	—CH3	—C2H5	—NH—(CH2)7—CH3
10	—CH3	—C2H5	—NH—(CH2)8—CH3
11	—CH3	—C2H5	—NH—(CH2)9—CH3
12	—CH3	—C2H5	—NH—(CH2)11—CH3
13	—CH3	—C2H5	—NH—(CH2)15—CH3
14	—CH3	—C2H5	5
15	—CH3	—C2H5	6
16	—CH3	—H	—NH—(CH2)7—NH2
17	—CH3	—H	—NH—(CH2)8—NH2
18	—CH3	—H	—NH—(CH2)9—NH2
19	—CH3	—H	—NH—(CH2)12—NH2
20	—CH3	—H	—NH—(CH2)6—CH3
21	—CH3	—H	—NH—(CH2)7—CH3
22	—CH3	—H	—NH—(CH2)8—CH3
23	—CH3	—H	—NH—(CH2)9—CH3
24	—CH3	—H	—NH—(CH2)11—CH3
25	—CH3	—H	—NH—(CH2)15—CH3
26	—CH3	—H	7
27	—CH3	—H	8
28	—CF3	—H	—NH—(CH2)7—NH2
29	—CF3	—H	—NH—(CH2)8—NH2
30	—CF3	—H	—NH—(CH2)9—NH2
31	—CF3	—H	—NH—(CH2)12—NH2
32	—CF3	—H	—NH—(CH2)6—CH3
33	—CF3	—H	—NH—(CH2)7—CH3
34	—CF3	—H	—NH—(CH2)8—CH3
35	—CF3	—H	—NH—(CH2)9—CH3
36	—CF3	—H	—NH—(CH2)11—CH3
37	—CF3	—H	—NH—(CH2)15—CH3
38	—CF3	—H	9
39	—CF3	—H	10
40	-isopropyl	—H	—NH—(CH2)7—NH2
41	-isopropyl	—H	—NH—(CH2)8—NH2
42	-isopropyl	—H	—NH—(CH2)9—NH2
43	-isopropyl	—H	—NH—(CH2)12—NH2
44	-isopropyl	—H	—NH—(CH2)6—CH3
45	-isopropyl	—H	—NH—(CH2)7—CH3
46	-isopropyl	—H	—NH—(CH2)8—CH3
47	-isopropyl	—H	—NH—(CH2)9—CH3
48	-isopropyl	—H	—NH—(CH2)11—CH3
49	-isopropyl	—H	—NH—(CH2)15—CH3
50	-isopropyl	—H	11
51	-isopropyl	—H	12
52	-tert-butyl	—H	—NH—(CH2)7—NH2
53	-tert-butyl	—H	—NH—(CH2)8—NH2
54	-tert-butyl	—H	—NH—(CH2)9—NH2
55	-tert-butyl	—H	—NH—(CH2)12—NH2
56	-tert-butyl	—H	—NH—(CH2)6—CH3
57	-tert-bulyl	—H	—NH—(CH2)7—CH3
58	-tert-butyl	—H	—NH—(CH2)8—CH3
59	-tert-butyl	—H	—NH—(CH2)9—CH3
60	-tert-butyl	—H	—NH—(CH2)11—CH3
61	-tert-butyl	—H	—NH—(CH2)15—CH3
62	-tert-butyl	—H	13
63	-tert-butyl	—H	14
64	—CH3	-isopropyl	—NH—(CH2)7—NH2
65	—CH3	-isopropyl	—NH—(CH2)8—NH2
66	—CH3	-isopropyl	—NH—(CH2)9—NH2
67	—CH3	-isopropyl	—NH—(CH2)12—NH2
68	—CH3	-isopropyl	—NH—(CH2)6—CH3
69	—CH3	-isopropyl	—NH—(CH2)7—CH3
70	—CH3	-isopropyl	—NH—(CH2)8—CH3
71	—CH3	-isopropyl	—NH—(CH2)9—CH3
72	—CH3	-isopropyl	—NH—(CH2)11—CH3
73	—CH3	-isopropyl	—NH—(CH2)15—CH3
74	—CH3	-isopropyl	15
75	—CH3	-isopropyl	16
76	—(CH2)2—O—CH3	—H	—NH—(CH2)7—NH2
77	—(CH2)2—O—CH3	—H	—NH—(CH2)8—NH2
78	—(CH2)2—O—CH3	—H	—NH—(CH2)9—NH2
79	—(CH2)2—O—CH3	—H	—NH—(CH2)12—NH2
80	—(CH2)2—O—CH3	—H	—NH—(CH2)6—CH3
81	—(CH2)2—O—CH3	—H	—NH—(CH2)7—CH3
82	—(CH2)2—O—CH3	—H	—NH—(CH2)8—CH3
83	—(CH2)2—O—CH3	—H	—NH—(CH2)9—CH3
84	—(CH2)2—O—CH3	—H	—NH—(CH2)11—CH3
85	—(CH2)2—O—CH3	—H	—NH—(CH2)15—CH3
86	—(CH2)2—O—CH3	—H	17
87	—(CH2)2—O—CH3	—H	18
88	—CH3	—C2H5	19
89	—CH3	—C2H5	—NH—(CH2)10—CH3
90	—CH3	—C2H5	—NH—(CH2)14—CH3
91	—CH3	—H	20
92	—CH3	—H	—NH—(CH2)10—CH3
93	—CH3	—H	—NH—(CH2)14—CH3
94	—CF3	—H	21
95	—CF3	—H	—NH—(CH2)10—CH3
96	—CF3	—H	—NH—(CH2)14—CH3
97	-isopropyl	—H	22
98	-isopropyl	—H	—NH—(CH2)10—CH3
99	-isopropyl	—H	—NH—(CH2)14—CH3

[0031] The novel 4-amino-2-(2-pyridyl)pyrimidines are prepared by methods known per se (J. Org. Chem.; 1967. 32, 1591). For that purpose, 2-cyanopyridine is reacted, in a suitable solvent, for example methanol, ethanol, isopropanol, DMF, tetrahydrofuran etc., with ammonium acetate or ammonium chloride at a temperature of from −10° C. to 100° C. over a period of from 1 hour to 24 hours to form the corresponding 2-amidinopyridine. The 2-amidinopyridine is then condensed with an appropriate β-keto ester using an auxiliary base, for example sodium carbonate, potassium hydroxide, sodium ethanolate, sodium methanolate, potassium tert-butanolate etc., in a suitable solvent, for example methanol, ethanol, butanol, tert-butanol, THF, DMF, acetonitrile, toluene, xylene etc., over a period of from 1 to 24 hours at a temperature of from 40 to 120° C. The 4-hydroxy-2-(2-pyridyl)pyrimidine thereby obtained is then converted into the corresponding 4-chloro-2-(2-pyridyl)pyrimidine by conventional methods using phosphorus oxychloride. The substituted 4-amino-2-(2-pyridyl)pyrimidines are obtained by reacting the 4-chloro-2-(2-pyridyl)pyrimidine with primary or secondary amines in a suitable solvent, for example DMF, dioxane, toluene, xylene, ethanol, butanol, and an auxiliary base, for example triethylamine, DIEA, sodium carbonate, potassium hydroxide etc., or using an excess of amine at from 40 to 130° C. over a period of from 1 to 24 hours. Preparation of the compounds of formula (2), except for the reaction with polymer-bound diamines, is analogous to that of compound (1). The polymer-bound diamines are obtained by reacting an excess of from 2 to 10 equivalents of diamine in, for example, DMF, dichloromethane, THF or dioxane with trityl chloride polystyrene resin at a temperature of from 10 to 50° C. over a period of from 0.5 to 24 hours. From 2 to 10 equivalents of appropriately substituted 4-chloro-2-(2-pyridyl)pyrimidines are then reacted, in a suitable solvent, for example dichloromethane, DMF, THF or toluene, with the polymer-bound diamines at from 10 to 120° C. over a period of from 2 to 48 hours. After washing the resin to remove the excess, the target compounds are split off using from 1 to 30% trifluoroacetic acid in dichloromethane at 25° C. over a period of from 1 to 5 hours. For the purpose of further purification, the substances are freeze-dried from tBuOH/water 4:1 with from 1 to 10% HOAc and once from tBuOH/water 4:1.

[0032] The entire reaction proceeds according to the following scheme: 23

[0033] The compounds of formula (3) are prepared analogously to preparation of the compounds of formula (1) according to the following scheme: 24

[0034] The 4-amino-2-(2-pyridyl)pyrimidines used in accordance with the invention exhibit pronounced antimicrobial action, especially against pathogenic gram-positive and gram-negative bacteria and against bacteria of the skin flora, and also against yeasts and moulds. They are accordingly suitable especially for disinfection, deodorisation, and for general and anti-microbial treatment of the skin and mucosa and of integumentary appendages (hair), more especially for the disinfection of hands and wounds.

[0035] They are accordingly suitable as antimicrobial active substances and preservatives in personal care preparations, for example shampoos, bath additives, haircare preparations, liquid and solid soaps (based on synthetic surfactants and salts of saturated and/or unsaturated fatty acids), lotions and creams, deodorants, other aqueous or alcoholic solutions, e.g. cleansing solutions for the skin, moist cleaning cloths, oils or powders.

[0036] The invention accordingly relates also to a personal care preparation comprising at least one compound of formula (1) and cosmetically tolerable carriers or adjuvants.

[0037] The personal care preparation according to the invention contains from 0.01 to 15% by weight, preferably from 0.1 to 10% by weight, based on the total weight of the composition, of a compound of formula (1), and cosmetically tolerable adjuvants.

[0038] Depending upon the form of the personal care preparation, it comprises, in addition to the 4-amino-2-(2-pyridyl)pyrimidine of formula (1), further constituents, for example sequestering agents, colourings, perfume oils, thickening or solidifying agents (consistency regulators), emollients, UV-absorbers, skin protective agents, antioxidants, additives that improve the mechanical properties, such as dicarboxylic acids and/or aluminium, zinc, calcium or magnesium salts of C14-C22fatty acids, and, optionally, preservatives.

[0039] The personal care preparation according to the invention may be in the form of a water-in-oil or oil-in-water emulsion, an alcoholic or alcohol-containing formulation, a vesicular dispersion of an ionic or non-ionic amphiphilic lipid, a gel, a solid stick or an aerosol formulation.

[0040] As a water-in-oil or oil-in-water emulsion, the cosmetically tolerable adjuvant contains preferably from 5 to 50% of an oil phase, from 5 to 20% of an emulsifier and from 30 to 90% water. The oil phase may comprise any oil suitable for cosmetic formulations, for example one or more hydrocarbon oils, a wax, a natural oil, a silicone oil, a fatty acid ester or a fatty alcohol. Preferred mono- or poly-ols are ethanol, isopropanol, propylene glycol, hexylene glycol, glycerol and sorbitol.

[0041] Cosmetic formulations according to the invention are used in various fields. There come into consideration, for example, especially the following preparations:

[0042] skin-care preparations, e.g. skin-washing and cleansing preparations in the form of tablet-form or liquid soaps, synthetic detergents or washing pastes,

[0043] bath preparations, e.g. liquid (foam baths, milks, shower preparations) or solid bath preparations, e.g. bath cubes and bath salts;

[0044] skin-care preparations, e.g. skin emulsions, multi-emulsions or skin oils;

[0045] cosmetic personal care preparations, e.g. facial make-up in the form of day creams or powder creams, face powder (loose or pressed), rouge or cream make-up, eye-care preparations, e.g. eyeshadow preparations, mascaras, eyeliners, eye creams or eye-fix creams; lip-care preparations, e.g. lipsticks, lip gloss, lip contour pencils, nail-care preparations, such as nail varnish, nail varnish removers, nail hardeners or cuticle removers;

[0046] intimate hygiene preparations, e.g. intimate washing lotions or intimate sprays;

[0047] foot-care preparations, e.g. foot baths, foot powders, foot creams or foot balsams, special deodorants and antiperspirants or callus-removing preparations;

[0048] light-protective preparations, such as sun milks, lotions, creams or oils, sun-blocks or tropicals, pre-tanning preparations or after-sun preparations;

[0049] skin-tanning preparations, e.g. self-tanning creams;

[0050] depigmenting preparations, e.g. preparations for bleaching the skin or skin-lightening preparations;

[0051] insect-repellents, e.g. insect-repellent oils, lotions, sprays or sticks;

[0052] deodorants, such as deodorant sprays, pump-action sprays, deodorant gels, sticks or roll-ons;

[0053] antiperspirants, e.g. antiperspirant sticks, creams or roll-ons;

[0054] preparations for cleansing and caring for blemished skin, e.g. synthetic detergents (solid or liquid), peeling or scrub preparations or peeling masks;

[0055] hair-removal preparations in chemical form (depilation), e.g. hair-removing powders, liquid hair-removing preparations, cream or paste-form hair-removing preparations, hair-removing preparations in gel form or aerosol foams;

[0056] shaving preparations, e.g. shaving soap, foaming shaving creams, non-foaming shaving creams, foams and gels, preshave preparations for dry shaving, aftershaves or aftershave lotions;

[0057] fragrance preparations, e.g. fragrances (eau de Cologne, eau de toilette, eau de parfum, parfum de toilette, perfume), perfume oils or perfume creams;

[0058] dental care, denture-care and mouth-care preparations, e.g. toothpastes, gel toothpastes, tooth powders, mouthwash concentrates, anti-plaque mouthwashes, denture cleaners or denture fixatives;

[0059] cosmetic hair-treatment preparations, e.g. hair-washing preparations in the form of shampoos and conditioners, hair-care preparations, e.g. pretreatment preparations, hair tonics, styling creams, styling gels, pomades, hair rinses, treatment packs, intensive hair treatments, hair-structuring preparations, e.g. hair-waving preparations for permanent waves (hot wave, mild wave, cold wave), hair-straightening preparations, liquid hair-setting preparations, hair foams, hairsprays, bleaching preparations, e.g. hydrogen peroxide solutions, lightening shampoos, bleaching creams, bleaching powders, bleaching pastes or oils, temporary, semi-permanent or permanent hair colorants, preparations containing self-oxidising dyes, or natural hair colorants, such as henna or camomile.

[0060] An antimicrobial soap has, for example, the following composition:

[0061] 0.01 to 5% by weight of a compound of formula (1)

[0062] 0.3 to 1% by weight titanium dioxide,

[0063] 1 to 10% by weight stearic acid,

[0064] soap base ad 100%, e.g. a sodium salt of tallow fatty acid or coconut fatty acid, or glycerol.

[0065] A shampoo has, for example, the following composition:

[0066] 0.01 to 5% by weight of a compound of formula (1),

[0067] 12.0% by weight sodium laureth-2-sulfate,

[0068] 4.0% by weight cocamidopropyl betaine,

[0069] 3.0% by weight NaCl and

[0070] water ad 100%.

[0071] A deodorant has, for example, the following composition:

[0072] 0.01 to 5% by weight of a compound of formula (1),

[0073] 60% by weight ethanol,

[0074] 0.3% by weight perfume oil, and

[0075] water ad 100%.

[0076] The invention relates also to an oral composition containing from 0.01 to 15% by weight, based on the total weight of the composition, of a compound of formula (1), and orally tolerable adjuvants.

[0077] Example of an Oral Composition:

[0078] 10% by weight sorbitol,

[0079] 10% by weight glycerol,

[0080] 15% by weight ethanol,

[0081] 15% by weight propylene glycol,

[0082] 0.5% by weight sodium lauryl sulfate,

[0083] 0.25% by weight sodium methylcocyl taurate,

[0084] 0.25% by weight polyoxypropylene/polyoxyethylene block copolymer,

[0085] 0.10% by weight peppermint flavouring,

[0086] 0.1 to 0.5% by weight of a compound of formula (1), and

[0087] 48.6% by weight water.

[0088] The oral composition according to the invention may be, for example, in the form of a gel, a paste, a cream or an aqueous preparation (mouthwash).

[0089] The oral composition according to the invention may also comprise compounds that release fluoride ions which are effective against the formation of caries, for example inorganic fluoride salts, e.g. sodium, potassium, ammonium or calcium fluoride, or organic fluoride salts, e.g. amine fluorides, which are known under the trade name Olafluor.

[0090] The 4-amino-2-(2-pyridyl)pyrimidines of formula (1) used in accordance with the invention are also suitable for treating, especially preserving, textile fibre materials. Such materials are undyed and dyed or printed fibre materials, e.g. of silk, wool, polyamide or polyurethanes, and especially cellulosic fibre materials of all kinds. Such fibre materials are, for example, natural cellulose fibres, such as cotton, linen, jute and hemp, as well as cellulose and regenerated cellulose. Preferred suitable textile fibre materials are made of cotton.

[0091] The 4-amino-2-(2-pyridyl)pyrimidines according to the invention are suitable also for treating, especially imparting antimicrobial properties to or preserving, plastics, e.g. polyethylene, polypropylene, polyurethane, polyester, polyamide, polycarbonate, latex etc. Fields of use therefor are, for example, floor coverings, plastics coatings, plastics containers and packaging materials; kitchen and bathroom utensils (e.g. brushes, shower curtains, sponges, bathmats), latex, filter materials (air and water filters), plastics articles used in the field of medicine, e.g. dressing materials, syringes, catheters etc., so-called “medical devices”, gloves and mattresses.

[0092] Paper, for example papers used for hygiene purposes, may also be provided with antimicrobial properties using the 4-amino-2-(2-pyridyl)pyrimidines according to the invention.

[0093] It is also possible for nonwovens, e.g. nappies/diapers, sanitary towels, panty liners, and cloths for hygiene and household uses, to be provided with antimicrobial properties in accordance with the invention.

[0094] The 4-amino-2-(2-pyridyl)pyrimidines of formula (1) are also used in washing and cleaning formulations, e.g. in liquid or powder washing agents or softeners.

[0095] The 4-amino-2-(2-pyridyl)pyrimidines of formula (1) can also be used especially in household and general-purpose cleaners for cleaning and disinfecting hard surfaces.

[0096] A cleaning preparation has, for example the following composition:

[0097] 0.01 to 5% by weight of a compound of formula (1)

[0098] 3.0% by weight octyl alcohol 4EO

[0099] 1.3% by weight fatty alcohol C8-C10polyglucoside

[0100] 3.0% by weight isopropanol

[0101] water ad 100%.

[0102] In addition to preserving cosmetic and household products, the preservation of technical products, the provision of technical products with antimicrobial properties and use as a biocide in technical processes are also possible, for example in paper treatment, especially in paper treatment liquors, printing thickeners of starch or cellulose derivatives, surface-coatings and paints.

[0103] The 4-amino-2-(2-pyridyl)pyrimidines of formula (1) are also suitable for the antimicrobial treatment of wood and for the antimicrobial treatment of leather, the preserving of leather and the provision of leather with antimicrobial properties.

[0104] The compounds according to the invention are also suitable for the protection of cosmetic products and household products from microbial damage.

[0105] The following Examples illustrate, but do not limit, the present invention.

PREPARATION EXAMPLES

Example 1

[0106] Synthesis of Substituted 4-amino-2-(2-pyridyl)pyrimidines

[0107] 1a: Preparation of 2-(2-pyridyl)pyrimidines 25

[0108] Sodium (1.44 g; 63 mmol) is dissolved, under nitrogen, in absolute ethanol (28.8 ml) at 45° C. A solution of 2-amidinopyridine hydrochloride (9.8 g; 63 mmol) in abs. ethanol (35 ml) is added and the mixture is heated at reflux for 1 hour. Portions, each of 8 ml, of the suspension are transferred to 8 flasks each containing a β-keto ester (7.88 mmol) in abs. ethanol (5 ml) (see Table 2 for amounts used). The suspensions are heated at reflux for 5 hours. After cooling to 25° C., the reaction mixture is evaporated to dryness and directly used in chlorination.

TABLE 2
β-Keto esters used
				Amount
β-Keto ester	R1	R2	R	used
2-ethylacetoacetic acid ethyl ester	—CH3	—C2H5	—C2H5	1.27 g
acetoacetic acid ethyl ester	—CH3	H	—C2H5	1.04 g
trifluoroacetoacetic acid methyl ester	—CF3	H	—CH3	1.36 g
4-methyl-3-oxopentanoic acid methyl	-isopropyl	—CF3	—CH3	1.27 g
ester
4,4-dimethyl-3-oxopentanoic acid	-tert-butyl	H	—CH3	1.27 g
methyl ester
2-isopropylacetoacetic acid ethyl ester	—CH3	—	—C2H5	1.38 g
		isopropyl
5-methoxy-3-oxopentanoic acid methyl	H3C—O—CH2—CH2—	H	—CH3	1.28 g
ester
benzoylacetic acid ethyl ester	phenyl	H	—C2H5	1.54 g
b: Synthesis of 4-chloro-2-(2-pyridyl)pyrimidines
26
27

[0109] The crude products from a) are taken up in phosphorus oxychloride (5 ml, 54 mmol, in each case) and heated at 110° C. for 3 hours. After cooling to 25° C., the reaction mixtures are poured into 10 ml of ice-water and slowly adjusted to pH 8-9 using aqueous sodium hydroxide solution. The crude products obtained are extracted with dichloromethane (5×10 ml) and the organic extracts are washed with water (2×10 ml) and with saturated NaCl solution. After drying over MgSO4, the product is filtered off and evaporated to dryness. Because of the good purity of most of the products (see Table 3), further processing is carried out without further purification.

TABLE 3
HPLC purity of crude products (detection at 214 nm)
No.	R1	R2	HPLC purity [%]
1	—CH3	-Et	>99
2	—CH3	—H	>99
3	—CF3	—H	>99
4	-isopropyl	—H	>99
5	-tert-butyl	—H	93
6	—CH3	-iPr	83
7	—CH2CH2—O—CH3	—H	98
8	-phenyl	—H	<5
c: Loading of trityl chloride-polystyrene resin (TCP) with diamines
28

[0110] In each case, 1 g of TCP resin (resin loading: 1.44 mmol/g) was shaken in abs. dichloromethane (5 ml) with a diamine (see Tab. 4; 5 equiv.; 7.2 mmol) for 24 hours at 25° C. The resin is washed with dichloromethane (5×), 1% HOAc/DCM, DMF and diethyl ether and dried in vacuo.

TABLE 4
Diamines used and weights thereof
diamine amount used
n = 7   938 mg
n = 8   1039 mg
n = 9   1140 mg
n = 12  1443 mg
d: Reaction of the diamine-TCP resins with 4-chloro-2-(2-pyridyl)pyrimidines
29
30
31

[0111] Diamine-TCP resins (50 mg; 72 μmol of diamine, in each case) are shaken in abs. dichloromethane (1 ml, in each case) with 4-chloro-2-(2-pyridyl)pyrimidines (3 equiv.; 216 μmol, in each case) and DIPEA (5 equiv.; 360 μmol) for 48 hours at 25° C. The resin is then filtered off, washed (DMF 5×, MeOH 5×, DCM 5×, diethyl ether 5×) and dried in vacuo. Cleaving is then carried out using 5% TFA/DCM (1.5 mL, in each case) for 1 hour at 25° C. The cleavage solutions are evaporated to dryness and the crude products are freeze-dried from tBuOH/water 4:1 with 10% HOAc and once from tBuOH/water 4:1.

[0112] e: Reaction of 4-chloro-2-(2-pyridyl)pyrimidines with Monoamines 32

[0113] 4-Chloro-2-(2pyridyl)pyrimidines (72 μmol, in each case) are heated with monoamines (3 equiv.; 216 μmol, in each case) in abs. dioxane (0.5 ml) for 24 hours at 90° C. After cooling to 25° C., in order to remove the excess of amine, a scavenger resin (polystyrene aldehyde resin; resin loading 1.28 mmol/g; 3 equiv; 216 mmol; 170 mg) and also trimethyl orthoformate (2 equiv.; 144 μmol; 15 mg; 16 μl) and additional abs. THF (2 ml) are added. The reaction mixture is shaken for 24 hours at 25° C. After filtering, the filtrate is evaporated to dryness and the crude product is freeze-dried from tBuOH/water 4:1 with 10% HOAc and once from tBuOH/water 4:1.

TABLE 5
Amines and amounts used
Amine	Amount (216 μmol)	Amine	Amount (216 μmol)
H2N-(CH2)6-CH3	25 mg; 32 μL	H2N-(CH2)10-CH3	37 mg; 46 μL
H2N-(CH2)7-CH3	28 mg; 36 μL	H2N-(CH2)14-CH3	49 mg
H2N-(CH2)8-CH3	31 mg; 40 μL	benzylamine	23 mg; 24 μL
H2N-(CH2)9-CH3	34 mg; 43 μL	cyclohexylamine	21 mg; 25 μL
H2N-(CH2)11-CH3	40 mg; 50 μL	phenylethylamine	26 mg; 29 μL
H2N-(CH2)15-CH3	52 mg

[0114] All compounds prepared by the methods described above are listed in Tab. 1 and were characterised by means of HPLC and MS (Table 7, Purities). Some of the compounds were analysed using 1H-NMR spectroscopy (Table 6):

TABLE 6
Comp.		1H-NMR 250 MHz (CD3OD), δ [ppm]:
of f.	Structure	δ [ppm]	Multiplicity	Assignment
(35)	33	0.80-1.75 3.64 6.87 7.78 8.26 8.63 8.76	m, 19 H t, 2 H ( J = 7 Hz) s, 1 H m, 1 H m, 1 H m, 1 H m, 1 H	—NH—CH—CH2—H arom. H arom. H arom. H arom. H arom. H arom.
(36)	34	0.80-1.75 3.63 6.87 7.73 8.21 8.62 8.77	m, 23 H t, 2 H (J = 7 Hz) s, 1 H m, 1 H m, 1 H m, 1 H m, 1 H	—NH—CH—CH2—H arom. H arom. H arom. H arom. H arom. H arom.
(37)	35	0.80-1.73 3.63 6.85 7.68 8.15 8.59 8.73	m, 31 H t, 2 H (J = 7 Hz) s, 1 H m, 1 H m, 1 H m, 1 H m, 1 H	—NH—CH—CH2—H arom. H arom. H arom. H arom. H arom. H arom.
(44)	36	0.80-1.80 3.72 6.61 7.73 8.13 8.58 8.87	m, 20 H t, 2 H (J = 7 Hz) s, 1 H m, 1 H m, 1 H m, 1 H m, 1 H	—NH—CH—CH2—H arom. H arom. H arom. H arom. H arom. H arom.
(47)	37	0.70-1.70 3.56 6.44 7.57 7.96 8.40 8.69	m, 26 H t, 2 H (J = 7 Hz) s, 1 H m, 1 H m, 1 H m, 1 H m, 1 H	—NH—CH—CH2—H arom. H arom. H arom. H arom. H arom. H arom.

Example 2

[0115] Determination of the Minimum Inhibitory Concentration (MIC Value) in Microtitre Plates

[0116] Nutrient Medium:

[0117] Casein-soybean flour-peptone broth for preparation of pre-cultures of test bacteria and yeast.

[0118] Mycological slant agar for the pre-culture of moulds

[0119] Examples of Test Organisms:

Bacteria: Staphylococcus hominis DMS 20328 (= SH)
          Escherichia coli NCTC 8196 (= EC)

[0120] Procedure:

[0121] The test substances are pre-dissolved in dimethyl sulfoxide (DMSO) and tested in a dilution series of 1:2.

[0122] Bacteria and yeast are cultured overnight in CASO broth, the mould is cultured overnight on mycological slant agar, and washed off using 10 ml of 0.85% sodium chloride solution (+0.1% Triton X-100).

[0123] All the test organisms are adjusted to an organism count of 1-5×106 CFU/ml using 0.85% sodium chloride solution.

[0124] The test substances are pre-pipetted into microtitre plates in amounts of 8 μl per well.

[0125] Pre-diluted organism suspensions are diluted 1:100 in CASO broth (bacteria and yeast) or Sabouraud 2% glucose broth (mould) and are added in amounts of 192 μl per well to the test substances.

[0126] The test batches are incubated for 48 hours at 37° C. (bacteria and yeast) or for 5 days at 28° C. (mould).

[0127] After incubation, the growth is determined on the basis of the turbidity of the test batches (optical density) at 620 nm in a microplate reader.

[0128] The minimum inhibitory concentration (MIC value) is the concentration of substance at which there is found (compared to the growth of the control) an appreciable inhibition of growth (≦20% growth) of the test organisms.

[0129] One microtitre plate is used for each test organism and substance concentration. All the substances are tested in duplicate.

[0130] The results are compiled in Table 7:

TABLE 7
Minimum inhibitory concentration (MIC) of compounds tested [μg]
Comp.
of		S. hominis	E. coli			S. hominis	E. coli
formula	Purity [%]	[μg/mL]	[μg/mL]	No.	Purity [%]	[μg/mL]	[μg/mL]
4	69	>120	>120	53	92	80	>80
5	63	>120	>120	54	99	30	120
6	63	n. d.	n. d.	55	66	7.5	60
7	60	120	120	56	89	3.75	>120
8	98	n. d.	n. d.	57	88	3.75	>120
9	74	7.5	>120	58	88	3.75	>120
10	79	5	>80	59	96	n. d.	n. d.
11	78	3.75	>120	60	94	3.75	120
12	98	7.5	120	61	71	>120	>120
13	95	120	>120	62	90	n. d.	n. d.
14	95	120	>120	63	90	3.75	>120
15	65	60	>120	64	80	30	>120
16	95	>120	>120	65	91	30	120
17	91	>80	>80	66	87	3.75	30
18	94	120	>120	67	82	5	20
19	93	15	60	68	83	7.5	>120
20	95	7.5	120	69	93	3.75	120
21	95	3.75	60	70	74	3.75	>120
22	95	3.75	120	71	71	5	>80
23	95	3.75	>120	72	77	15	>120
24	93	2.5	80	73	67	120	>120
25	90	15	>120	74	85	60	>120
26	95	60	>120	75	53	30	>120
27	96	60	>120	76	95	40	>80
28	98	>80	>80	77	92	30	120
29	>99	>80	>80	78	64	15	60
30	99	120	120	79	69	10	20
31	98	5	20	80	71	30	>120
32	99	3.75	>120	81	87	15	>120
33	99	2.5	>80	82	70	7.5	120
34	99	3.75	>120	83	65	7.5	>120
35	99	3.75	>120	84	56	15	120
36	98	60	>120	85	73	120	120
37	90	>120	>120	86	75	120	>120
38	99	60	>120	87	62	30	120
39	99	30	>120	88	90	120	120
40	75	120	>120	89	91	3.75	>120
41	94	120	>120	90	87	30	120
42	99	30	120	91	80	30	>120
43	91	7.5	60	92	84	3.75	>120
44	97	3.75	>120	93	83	7.5	>120
45	97	3.75	>120	94	83	>120	>120
46	97	3.75	>120	95	79	120	>120
47	97	5	>80	96	88	120	120
48	80	3.75	120	97	90	15	120
49	90	>120	>120	98	81	3.75	>120
50	94	15	>120	99	80	15	>120
51	95	30	>120
52	80	n. d.	n. d.
n. d. = not determined
Purity [%] = HPLC area percentages at a detection wavelength of 214 nm

[0131]

TABLE 8
Minimum inhibitory concentrations (MIC in [μg/mL]) of selected compounds with
respect to further microorganisms
	Compound of formula
Microorganism	37	44	68	45	46	70	23	47	63	71	32
Staphylococcus aureus ATCC 9144	15*	15	15	7.5	30	7.5	7.5	7.5	15	7.5	15
S. epidermidis ATCC 12228	7.5	15	15	7.5	30	7.5	7.5	3.75	30	7.5	7.5
Micrococcus luteus ATCC 9341	1.0	1.0	3.75	3.75	3.75	1.0	<0.5	<0.5	7.5	1.9	>0.5
Enterococcus hirae ATCC 10541	30	15	7.5	3.75	30	3.75	3.75	3.75	30	7.5	7.5
E. coli NCTC 8196	>30	>30	>30	>30	>60	>60	>60	>60	*60	>60	>60
Epidermophyton floccosum DSM 10709	>60	60	60*	>60	60	*7.5	*15	*60	*15	60	*60
Trichophyton mentagrophytes ATCC 9533	>60	>60	>60	>60	>60	15	15	60	*60	*30	>60
	Compound of formula
Microorganism	56	9	33	57	10	34	58	11	35	43	83
Staphylococcus aureus ATCC 9144	3.75	15	15	1.9	15	>60	*1.9	7.5	*7.5	30	*15
S. epidermidis ATCC 12228	3.75	*15	3.75	1.9	15	*60	1.9	7.5	7.5	*15	15
Micrococcus luteus ATCC 9341	<0.5	3.75	<0.5	<0.5	<0.5	>0.5	<0.5	1.9	<0.5	3.75	3.75
Enterococcus hirae ATCC 10541	1.9	30	15	1.0	30	60	7.5	7.5	7.5	30	30
E. coli NCTC 8196	>60	>60	>60	>60	>60	>60	>60	>60	>60	>60	>60
Epidermophyton floccosum DSM 10709	*30	*15	>60	60	60	>60	>60	>60	>60	>60	>60
Trichophyton mentagrophytes ATCC 9533	*30	*30	>60	*60	60	>60	>60	>60	>60	>60	>60
	Compound of formula
Microorganism	12	60	21	69	77	22	30	54	78	7
Staphylococcus aureus ATCC 9144	15	15	120	7.5	>60	7.5	>60	*60	*60	>60
S. epidermidis ATCC 12228	*7.5	*7.5	120	7.5	>60	<3.75	>60	>60	*60	>60
Micrococcus luteus ATCC 9341	<3.75	<3.75	120	<3.75	>60	<3.75	60	15	>60	>60
Enterococcus hirae ATCC 10541	7.5	7.5	120	3.75	>60	<3.75	>60	>60	>60	>60
E. coli NCTC 8196	>60	>60	120	>60	>60	>60	>60	>60	>60	>60
Klebsiella pneumonia ATCC 4352	>60	>60	120	*60	>60	>60	>60	>60	>60	>60
Salmonella choleraesuis ATCC 10708	>60	>60	>120	>60	>60	>60	>60	>60	>60	>60
Pseudomonas aeruginosa CIP A-22	>60	>60	>120	>60	>60	>60	>60	>60	>60	>60
Candida albicans ATCC 10231	*60	>60	120	60	>60	15	>60	>60	>60	>60
Aspergillus niger ATCC 6275	>60	60	>60	*60	>60	60	>60	>60	>60	>30
Epidermophyton floccosum DSM 10709	*30	>60	>60	15	>60	*7.5	>60	>60	>60	>30
Trichophyton mentagrophytes ATCC 9533	60	>60	>60	*15	>60	15	>60	>60	>60	>30
	Compound of formula
Microorganism	79	24	48	65	18	42	66	19
Staphylococcus aureus ATCC 9144	*60	<3.75	<3.75	>60	>60	60	60	60
S. epidermidis ATCC 12228	30	3.75	<3.75	>60	>60	60	60	60
Micrococcus luteus ATCC 9341	60	<3.75	<3.75	>60	>60	*15	*30	7.5
Enterococcus hirae ATCC 10541	>60	*15	<3.75	>60	>60	>60	>60	60
E. coli NCTC 8196	60	>60	>60	>60	>60	>60	>60	>60
Klebsiella pneumonia ATCC 4352	>60	>60	>60	>60	>60	>60	>60	>60
Salmonella choleraesuis ATCC 10708	>60	>60	>60	>60	>60	>60	>60	>60
Pseudomonas aeruginosa CIP A-22	>60	>60	>60	>60	>60	>60	>60	>60
	Compound of formula
Microorganism	79	24	48	65	18	42	66	19
Candida albicans ATCC 10231	*30	30	*15	>60	>60	>60	>60	7.5
Aspergillus niger ATCC 6275	>60	>60	>60	>60	>60	>60	>60	>60
Epidermophyton floccosum DSM 10709	>60	60	*60	>60	>60	>60	>60	>60
Trichophyton mentagrophytes ATCC 9533	>60	>60	*60	>60	>60	>60	>60	>60
	Compound of formula
Microorganism	92	93	89	90	98	99
Staphylococcus aureus ATCC 9144	3.75	15	7.5	*30	1.9	7.5
S. epidermidis ATCC 12228	*1.9	7.5	7.5	*30	1.9	3.75
Micrococcus luteus ATCC 9341	<0.5	1.9	1.9	15	<0.5	1.0
Enterococcus hirae ATCC 10541	3.75	15	7.5	30	1.9	3.75
E. coli NCTC 8196	>120	>120	>60	>60	120	>60
Klebsiella pneumonia ATCC 4352	120	>120	60	>60	120	>60
Salmonella choleraesuis ATCC 10708	>120	>120	>60	>60	>120	>60
Pseudomonas aeruginosa CIP A-22	>120	>120	>60	>60	>120	>60
Candida albicans ATCC 10231	15	*120	15	*60	30	>60
Aspergillus niger ATCC 6275	*60	>60	30	>120	>60	>60
Epidermophyton floccosum DSM 10709	15	>60	15	60	15	*60
Trichophyton mentagrophytes ATCC 9533	15	>60	*7.5	>120	>60	>60
*significant inhibition of growth, but not complete inhibition

Example 3

[0132] Determination of the Bactericidal Activity of Selected Compounds

[0133] Test Method:

[0134] Nutrient Medium:

[0135] Casein-soybean flour-peptone broth for preparation of pre-cultures of test bacteria

[0136] Examples of test Organisms:

[0137] Staphylococcus aureus ATCC 6538

[0138] Escherichia coli ATCC 10536

[0139] Salmonella choleraesuis ATCC 10708

[0140] Procedure:

[0141] The test substances are dissolved in dimethyl sulfoxide (DMSO) and tested in a concentration of 120 μg/ml.

[0142] Bacteria are incubated overnight in CASO broth and adjusted to an organism count of 1-5×105 CFU/ml using 0.85% sodium chloride solution.

[0143] The test substances are pre-pipetted into microtitre plates in amounts of 8 μl per well.

[0144] The adjusted test organism suspensions are added in amounts of 192 μl per well to the test substances and mixed. After defined contact times, the test batches are mixed, an aliquot is withdrawn and diluted in several steps in a dilution series of 1:10 in a suitable inactivation medium.

[0145] The test plates are incubated for 24 hours at 37° C.

[0146] After incubation, the growth is determined on the basis of the turbidity of the test batches (optical density) at 620 nm in a microplate reader.

[0147] On the basis of the number of growth-exhibiting steps in the dilution series, the reduction in the test organism concentration is determined in powers of ten (log value).

[0148] One microtitre plate is used for each test organism.

[0149] All the substances are tested in duplicate.

TABLE 9
Logarithmic reduction in organism count after contact for 30
minutes at a substance concentration of 120 μg/mL
	Microorganism
	S. choleraesuis	E. coli	S. aureus
Compound of formula	ATCC 10708	NCTC 8196	ATCC 6538
(12)	<1	<1	1-2
(37)	<1	<1	1-2
(44)	<1	<1	1-2
(60)	<1	<1	<1
(68)	<1	<1	≦1
(21)	<1	<1	1-2
(45)	<1	<1	1-2
(69)	≦1	<1	1-2
(77)	<1	<1	>3
(22)	<1	<1	1-2
(30)	≦1	<1	≦1
(46)	<1	<1	≦1
(54)	≦1	≦1	2-3
(70)	<1	≦1	≦1
(78)	<1	≦1	>3
(7)	<1	≦1	2
(23)	<1	<1	3
(47)	<1	<1	1
(63)	<1	≦1	<1
(71)	<1	<1	2
(24)	<1	≦1	2
(32)	≦1	<1	<1
(48)	<1	<1	1
(57)	<1	<1	1
(9)	<1	<1	<1
(33)	<1	<1	<1
(57)	<1	<1	<1
(65)	<1	<1	3
(10)	<1	<1	1-2
(18)	<1	<1	1
(34)	<1	<1	<1
(42)	<1	≦1	2
(58)	≦1	≦1	1
(11)	<1	≦1	1-2
(19)	<1	≦1	2-3
(35)	<1	<1	<1
(43)	<1	<1	3
(83)	<1	≦1	2
(92)	≦1	<1	>3
(93)	<1	≦1	2
(89)	≦1	≦1	2
(90)	<1	<1	2
(98)	2-3	1-2	1
(99)	<1	≦1	2

Claims

1. A compound of formula

2. A compound according to claim 1, wherein R5 and R6 are each independently of the other hydrogen or C1-C20alkyl.

3. A compound according to claim 1, wherein R5 and R6 are hydrogen.

4. A compound according to 3, wherein R1 and R2 are each independently of the other hydrogen, unsubstituted or mono- or poly-halo-substituted C1-C20alkyl; or C1-C5alkoxy-C1-C5alkyl.

5. A compound according to claim 4, wherein R1 and R2 are each independently of the other hydrogen, unsubstituted or mono- or poly-halo-substituted C1-C5alkyl; or C1-C3alkoxy-C1-C5alkyl.

6. A compound according claim 5, wherein R1 and R2 are each independently of the other hydrogen, methyl, ethyl, isopropyl, tert-butyl, CF3or the radical —(CH2)m—O—CH3, and m is from 1to 4.

7. A compound according to claim 1, wherein R3 is unsubstituted or amino-substituted C7-C20alkyl, C3-C7cycloalkyl-C8-C20alkyl, C1-C4alkoxy-C7-C20alkyl, R7R8N—C7C20alkyl, phenyl-C1-C4alkyl or phenyl-C1-C6alkoxy; and R1, R2, R4, R5, R6, R7 and R8 are as defined in claim 1.

8. A compound according to claim 7, wherein R3 is unsubstituted or amino-substituted C7-C20alkyl or R7R8N—C7-C20alkyl.

9. A compound according to claim 7, wherein R3 is phenyl-C1-C3alkyl.

10. A compound according to claim 1, wherein R1 and R2 are each independently of the other hydrogen, methyl, ethyl, isopropyl, tert-butyl, CF3or the radical —(CH2)m—O—CH3; R3 is unsubstituted or amino-substituted C7-C20alkyl or R7R8N—C7-C20alkyl; R4 is hydrogen; or C1-C20alkyl; R5, R6, R7 and R8 are each independently of the others hydrogen or C1-C20alkyl; and m is from 1 to 4.

11. A compound according to claim 10, wherein R5, R6, R7 and R8 are hydrogen.

12. A compound according to claim 1, which corresponds to formula

13. A compound according to claim 1, which corresponds to formula

14. A process for the preparation of a compound of formula

15. A process for the preparation of a compound of formula

16. An antimicrobial method for the treatment of surfaces, which comprises contacting a substrate with an antimicrobially effective amount of a compound of formula (1) according to claim 1.

17. A method according to claim 16 for the antimicrobial treatment, deodorisation and disinfection of the skin, mucosa and hair, which comprises applying an antimicrobially effective amount of a compound of the formula (1) according to claim 1 thereto.

18. A method according to claim 16, wherein the compound of formula (1) is used for disinfection and deodorisation.

19. A method according to claim 16 for the antimicrobial treatment of textile fibre materials, which comprises applying an antimicrobially effective amount of the compound of formula (1) according to claim 1 thereto.

20. A method according to claim 16, wherein the compound of formula (1) is used for preservation.

21. A method according to claim 16 which comprises applying an antimicrobially effective amount of formula (1) in washing and cleaning formulations.

22. A method according to claim 16 which comprises the incorporation of an effective amount of a compound of formula (1) according to claim 1 into materials selected from plastics, paper, nonwovens, wood or leather for the antimicrobial finishing of said materials.

23. A personal care preparation comprising from 0.01 to 15% by weight, based on the total weight of the composition, of a compound of formula (1), and cosmetically tolerable adjuvants.

24. An oral composition comprising from 0.01 to 15% by weight, based on the total weight of the composition, of a compound of formula (1), and orally tolerable adjuvants.