4-aminobutyric acid compounds, compositions and methods of use for treating disorders related to a dysfunction of GABA.sub.B receptors

The invention relates to new 4-aminobutyric acid compounds.
Numerous arylpropionic compounds are known from the literature that have, in particular, analgesic and anti-inflammatory properties. Also known is baclofen or 4-amino-3-(4-chlorophenyl)butyric acid, a compound agonistic to GABA.sub.B receptors which is used in human therapeutics on account of its antispastic properties. Other 4-amino-3-arylbutyric acids, especially heteroarylbutyric compounds, which exhibit an affinity to the GABA.sub.B receptor, have also been described (J. Med. Chem. 1987, 30, 743-746).
In comparison with the compounds of the prior art, the compounds of the present invention exhibit a very selective and distinctly greater affinity. In addition, some of them antagonise the excitation induced by convulsants at doses lower than that of baclofen. Others exhibit the property of stimulating the synthesis of cyclic AMP in the cerebral cortex, and therefore of increasing the metabolic capacities of the brain. The intensity of that affinity to the GABA.sub.B receptor and the strength of their activity renders possible, in human or animal therapeutics, the administration of lower doses of the compounds of the invention. This reduced dosage goes hand in hand with a reduction in the side effects that are observed with less active compounds since it is generally recognised that toxicity has no connection with the mechanism of pharmacological action but depends essentially on the chemical structure of the compounds. Thus, small amounts of the compounds of the invention produce an effect comparable to that obtained with much greater amounts of the compounds of the prior art; the risks of toxic effects, especially hepatic ones, are greatly reduced. That advantage is particularly valuable in the case of the weak populations for which the compounds are intended, generally individuals suffering from spastic disorders or elderly individuals (Alzheimer's disease, individuals suffering from senile dementia or affected by disorders connected with senescence), these populations often already suffering from disorders of the hepatic functions.
More specifically, the invention relates to new 4-aminobutyric acid compounds corresponding to the general formula (I): ##STR2## in which: R.sub.1 represents a hydroxy, amino, lower alkylamino or lower alkoxy group, or a halogen atom,
R.sub.2 represents a hydrogen atom, a lower alkyl radical, a lower acyl radical, or a lower alkoxycarbonyl radical,
R represents:
a radical of the formula ##STR3## in which: X represents an oxygen or Sulphur atom or an NH group,
Y represents a carbon, oxygen or nitrogen atom,
R'.sub.1 and R'.sub.2, which are identical or different, represent a halogen or hydrogen atom or a lower alkyl, lower alkoxy, hydroxy, nitro, amino, lower alkylamino or trifluoromethyl radical,
with the proviso that, when X is an oxygen atom, Y is a carbon atom and each of R'.sub.1 and R.sub.2 is a hydrogen atom, then R'.sub.2 can represent neither a hydrogen atom nor a methoxy group,
a radical of the formula ##STR4## in which: Z represents an oxygen or sulphur atom or an NH group,
T represents a carbon or nitrogen atom, R'.sub.3 and R'.sub.4, which are identical or different, represent a radical selected from hydrogen, halogen, lower alkyl, lower alkoxy, hydroxy, nitro, amino, lower alkylamino or trifluoromethyl, with the proviso that, when Z is a sulphur atom, T is a carbon atom and
R'.sub.3 is a hydrogen atom, R'.sub.4 may not be a methyl grouping or a hydrogen, chlorine or bromine atom, and that, when Z is an oxygen atom, T is a carbon atom and R'.sub.3 is a hydrogen atom, R'.sub.4 may not be a hydrogen atom or a methyl group,
a cycloalkyl radical having 4 or 5 carbon atoms or a cycloalkylalkyl or dicycloalkylalkyl radical having from 4 to 16 carbon atoms, optionally substituted at rings level by a group selected from halogen, hydroxy, lower alkyl, lower alkoxy, nitro, amino, lower alkylamino or trifluoromethyl,
an optionally substituted aromatic radical having 6 ring members that includes 2 or 3 nitrogen atoms in its carbon skeleton,
an aromatic radical having 6 ring members that includes from 1 to 3 nitrogen atoms in its carbon skeleton and is fused to a benzene ring, it being optionally possible for each of these 2 rings to be substituted,
or a saturated or unsaturated ring having seven ring members that includes one or two nitrogen atoms in its carbon skeleton and is optionally fused to a benzene ring and is optionally substituted on the nitrogen and/or benzene ring,
the term "substituted" indicating that the groups so qualified can be substituted by one or more groups selected from halogen atom, lower alkyl, lower alkoxy, hydroxy, trifluoromethyl, nitro, amino or lower alkylamino
their optical isomers and also, where appropriate, their salts of addition with a pharmaceutically acceptable base or acid,
it being understood that, unless otherwise indicated, the terms "lower alkyl", "lower alkoxycarbonyl", "lower alkoxy", "lower alkylamino" and "lower acyl" indicate groups containing from 1 to 6 carbon atoms in a straight or branched chain.
Of the pharmaceutically acceptable acids or bases that can be used to convert the compounds of the invention into salts there may be mentioned, by way of non-limiting examples, hydrochloric, hydrobromic, sulphuric, nitric, oxalic, malic, maleic, succinic, tartaric, methanesulphonic, camphoric and camphosulphonic acid, sodium hydroxide, potassium hydroxide, triethylamine, diethylamine, ethanolamine or diethanolamine, arginine, lysine, . . .
The process for the preparation of the compounds of formula (I) is characterised in that there is used as starting material a compound of formula (II): ##STR5## in which R is as defined in formula (I), which can be: either hydrolysed by the action of a metal hydroxide to obtain, after optional purification, a compound of formula (I/a): ##STR6## a particular form of the compounds of formula (I) in which R.sub.1 represents a hydroxy group, R.sub.2 represents a hydrogen atom and R is as defined in formula (I),
or converted by the action of a lower alkyl dicarbonate in the presence of a strong base into a compound of formula (III): ##STR7## in which R is as defined above and R.sub.23 represents a lower alkoxycarbonyl group, which is treated, after optional purification, with an alkali metal hydroxide in an anhydrous medium, and then with an acid to obtain a compound of formula (I/b): ##STR8## in which R and R.sub.23 are as defined above, a particular form of the compounds of formula (I) in which R.sub.1 represents a hydroxy group, R.sub.2 represents a lower alkoxycarbonyl radical and R is as defined in formula (I), which, treated in an acidic medium, yields a compound of formula (I/a) such as defined above,
which compound of formula (I/a), irrespective of the process by which it has been obtained, may, if desired, be converted by a halogenating agent into its halide of formula (I/c): ##STR9## in which Hal represents a halogen atom and R is as defined in formula (I),
a particular form of the compounds of formula (I) in which R.sub.1 represents a halogen atom, R.sub.2 represents a hydrogen atom and R is as defined in formula (I),
which compound of formula (I/a) or (I/c) can be treated, if desired:
with a compound of the formula:
R.sub.1 "--H
in which R.sub.1 " represents an amino, lower alkylamino or lower alkoxy group,
to yield a compound of formula (I) in which R.sub.1 represents an amino, lower alkylamino or lower alkoxy group,
and, if desired, with an alkylating agent such as dimethyl sulphate or an alkyl halide of the formula:
R.sub.21 --X
in which R.sub.21 represents a lower alkyl group and X represents a halogen atom,
to yield a compound of formula (I) in which R.sub.2 represents a lower alkyl group,
or, if desired, with an acid chloride of the formula:
R.sub.22 Cl
or an acid anhydride of the formula:
R.sub.22 OR.sub.22
R.sub.22 representing a lower acyl grouping,
to yield a compound of formula (I) in which R.sub.2 represents a lower acyl group,
the compounds of formula (I) then, if desired, being either resolved into their optical isomers and then converted into salts by the addition of a pharmaceutically acceptable base or acid, or converted directly into salts in racemic form by the addition of a pharmaceutically acceptable base or acid.
The compounds of formula (II) can be obtained:
either by condensing, in an aprotic apolar solvent, an aldehyde of formula (V):
R--CHO (V)
in which R is as defined in formula (I),
with a carboxymethylidenetriphenylphosphorane ester of formula (VI):
(C.sub.6 H.sub.5).sub.3 --P.dbd.CH--COOR' (VI)
in which R' represents a lower alkyl radical,
to obtain an ester of formula (VII):
R--CH.dbd.CH--COOR' (VII)
in which R and R' are as defined above,
which is condensed in a protic polar medium with nitromethane in the presence of a strong base, to obtain a compound of formula (VIII): ##STR10## in which R and R' are as defined above, which is reduced in an alcoholic medium by the action of hydrogen in the presence of a metal catalyst, to form a compound of formula (IX): ##STR11## in which R and R' are as defined above, which is cyclised by heating to form a compound of formula (II): ##STR12## in which R is as defined in formula (I), or by treating a compound of formula (X):
RCOCH.sub.3 (X)
in which R is as defined in formula (I),
at elevated temperature and in the presence of zinc with a compound of formula (XI):
Br--CH.sub.2 --COOA (XI)
in which A represents a lower alkyl grouping,
to yield, after optional acidic hydrolysis, extraction and purification, a compound of formula (XII): ##STR13## in which R and A are as defined above, which is treated with N-bromosuccinimide to yield a compound of formula (XIII): ##STR14## in which R and A are as defined above, which is treated with ammonia, preferably an excess thereof, to yield a compound of formula (XIV): ##STR15## in which R is as defined above, which is subjected to catalytic hydrogenation to obtain a compound of formula (II) as defined above.
The compounds of formula (II), wherein R does not represent:
a 2-benzofuryl group substituted in the benzene nucleus by a chlorine atom, a bromine atom or a methoxy group,
or a 2-benzothienyl group,
and the compounds of formula (III) are new and are an integral part of the present invention in their capacity as starting materials that can be used for the synthesis of the compounds of the invention.
The compounds of formula (I) possess valuable pharmacological properties. They have a very great and selective affinity to the GABA.sub.B receptor, which is greater than that of the compounds of the prior art.
Some of them exhibit a GABA.sub.B receptor-antagonising activity and can therefore be administered in the treatment of memory disorders, mental disorders connected with senescence, and also in the treatment of Alzheimer's disease.
Other compounds, on the other hand, exhibit an agonist activity and are therefore suitable for spastic individuals or individuals suffering from Angina pectoris.
The present invention also relates to pharmaceutical compositions containing the compounds of formula (I) or one of their salts of addition with a pharmaceutically acceptable acid or base, alone or in combination with one or more pharmaceutically acceptable inert, non-toxic excipients or carriers.
Of the pharmaceutical compositions according to the invention there may be mentioned more particularly those that are suitable for oral, parenteral, nasal, rectal, perlingual, ocular or pulmonary administration, and especially injectable preparations, aerosols, eye or nose drops, tablets or dragees, sublingual tablets, soft gelatin capsules, sachets, suppositories, creams, ointments, dermal gels, . . .
The dosage varies according to the age and weight of the patient, the route of administration, the nature of the disorder and any associated treatment and ranges from 1 mg to 1 gram per 24 hours.
The following Examples illustrate the invention and do not limit it in any way.
The starting materials are described in the literature or can be prepared in a similar manner
The 1H nuclear magnetic resonance spectra (NMR) were produced using tetramethylsilane (TMS) as the internal reference. The chemical displacements are expressed in parts per million (ppm).
The infrared spectra were effected in the form of potassium bromide discs containing approximately 1% of the product to be analysed.

EXAMPLE 1
3-(2-(5-isopropylbenzofuryl))-4-aminobutanoic acid
STAGE A: ethyl 3-(2-(5-isopropylbenzofuryl))propenoate
A solution containing 0.1 mol of 2-(5-isopropyl)benzofurylcarbaldehyde and 0.1 mol of ethoxycarbonylmethylidenetriphenylphosphorane in 200 cm.sup.3 of benzene is heated under reflux for 4 hours under a nitrogen atmosphere. It is then cooled and the solvent is evaporated under reduced pressure. The residue is taken up in 200 cm.sup.3 of ether, the insoluble material is removed by suction-filtering and the filtrate is evaporated to dryness and then the evaporation residue is distilled under reduced pressure. The compound of stage A is obtained:
b.p. (3 mm Hg)=186.degree. C.
STAGE B: ethyl 3-(2-(5-isopropylbenzofuryl)- 4-nitrobutanoate
0.05 mol of the compound obtained in stage A of Example 1 is heated for 18 hours at 70.degree. C. in 50 cm.sup.3 of nitromethane and 2 cm.sup.3 of a 40% methanolic solution of Triton B. The whole is cooled, neutralised with a molar solution of hydrochloric acid and extracted with ether. The ethereal phase is washed with water, dried, filtered and then evaporated to dryness. The compound of stage B is obtained after purification by high performance liquid chromatography.
STAGE C: 4-(2-(5-isopropylbenzofuryl))-2-oxopyrrolidine
0.05 mol of the compound obtained in stage B of Example 1 is reduced in an ethanolic solution by hydrogen at atmospheric pressure and at ambient temperature in the presence of Raney nickel. The whole is filtered and evaporated under reduced pressure, and then the residue is heated for 2 hours and recrystallised from petroleum ether.
Melting point: 151.degree. C.
Spectral Characteristics
infrared: 1,690 cm.sup.-1 : .nu. CO 3,300 m.sup.-1 : .nu. NH
NMR (CDCl.sub.3): .delta.: 1.26 ppm: doublet: ((CH.sub.3).sub.2 C) .delta.: 2.75 ppm: doublet: (CH.sub.2 CO) .delta.: 2.80-3.20 ppm: multiplet: (CH (CH.sub.3).sub.2) .delta.: 3.40-4.10 ppm: multiplet: (CH, CH.sub.2 N) .delta.: 5.70 ppm: singlet: (NH) .delta.: 6.50 ppm: singlet: (H.sub.3 ', benzofuran) .delta.: 7.00-7.50 ppm: multiplet: (H.sub.4 ', H.sub.6 ', H.sub.7 '; benzofuran)
STAGE D: 3-(2-(5-isopropylbenzofuryl))-4-aminobutanoic acid
0.01 mol of the compound obtained in stage C of Example 1 is heated under reflux for one hour in 20 cm.sup.3 of 95% ethanol in the presence of 5 cm.sup.3 of 40% sodium hydroxide solution. The whole is cooled and evaporated to dryness. The residue is taken up in from 15 to 20 cm.sup.3 of water that has been acidified to pH=1 by 10% HCl. The whole is evaporated to dryness, taken up in from 1 to 3 cm.sup.3 of trifluoroacetic acid and chromatographed over an ion exchange resin (DOWEX 50 WH+) using a 5% ammonium hydroxide solution as eluant. The eluant is evaporated to dryness and the residue is recrystallised from ethanol.
Melting point: 190.degree. C.
Spectral Characteristics
infrared: 1,580 cm.sup.-1 : .nu. CO 2,300-3,200 cm.sup.-1 : .nu. OH
EXAMPLE 2
3-(2-(5-methylbenzofuryl))-4-aminobutanoic acid
By following the instructions in stages A to D of Example 1, but replacing the 2-(5-isopropyl)benzofurylcarbaldehyde in stage A of Example 1 by 2-(5-methyl)benzofurylcarbaldehyde, there are obtained in succession, after a purification stage by high performance liquid chromatography, as appropriate, the following compounds:
STAGE A: ethyl 3-(2-(5-methylbenzofuryl))propenoate
Melting point: 73.degree. C.
STAGE B: ethyl 3-(2-(5-methylbenzofuryl))-4-nitrobutanoate
STAGE C: 4-(2-(5-methylbenzofuryl))-2-oxopyrrolidine
Melting point: 151.degree. C.
Spectral Characteristics
infrared: 1,690 cm.sup.-1 : .nu. CO 3,300 m.sup.-1 : .nu. NH
NMR (CDCl.sub.3): .delta.: 2.41 ppm: singlet: (CH.sub.3) .delta.: 2.69 ppm: doublet: (CH.sub.2 CO) .delta.: 3.50-4.10 ppm: multiplet: CH.sub.2 N, CH) .delta.: 6.00 ppm: singlet: (NH) .delta.: 6.46 ppm: singlet: (H.sub.3 ', benzofuran) .delta.: 6.90-7.50 ppm: multiplet: (H.sub.4 ', H.sub.6 ', H.sub.7 '; benzofuran)
STAGE D: 3-(2-(5-methylbenzofuryl))-4-aminobutanoic acid
Melting point: 191.degree. C.
Spectral Characteristics
infrared: 1,580 cm.sup.-1 : .nu. CO 2,300-3,200 cm.sup.-1 : .nu. (COO--, NH.sub.3.sup.+)
EXAMPLE 3
3-(2-(5-ethylbenzofuryl))-4-aminobutanoic acid
By following the instructions in stages A to D of Example 1, but replacing the 2-(5-isopropyl)benzofurylcarbaldehyde in stage A of Example 1 by 2-(5-ethyl)benzofurylcarbaldehyde, there are obtained in succession, after a purification stage by high performance liquid chromatography, as appropriate, the following compounds:
STAGE A: ethyl 3-(2-(5-ethylbenzofuryl))propenoate
Melting point: 52.degree. C.
STAGE B: ethyl 3-(2-(5-ethylbenzofuryl))-4-nitrobutanoate
STAGE C: 4-(2-(5-ethylbenzofuryl))-2-oxopyrrolidine
Melting point: 125.degree.-127.degree. C.
Spectral Characteristics
infrared: 1,670 cm.sup.-1 : .nu. CO 3,200 m.sup.-1 : .nu. NH
NMR (CDCl.sub.3): .delta.: 1.25 ppm: triplet: (CH.sub.2 --CH.sub.3) .delta.: 2.50-3.00 ppm: multiplet: (CH.sub.2 --CH.sub.3, CH.sub.2 CO) .delta.: 3.40-4.10 ppm: multiplet: (CH.sub.2 N, CH) .delta.: 5.93 ppm: singlet: (NH) .delta.: 6.46 ppm: singlet: (H.sub.3 ', benzofuran) .delta.: 7.00-8.00 ppm: multiplet: (H.sub.4 ', H.sub.6 ', H.sub.7 '; benzofuran)
STAGE D: 3-(2-(5-ethylbenzofuryl))-4-aminobutanoic acid
Melting point: 195.degree. C.
Spectral Characteristics
infrared: 1,580 cm.sup.-1 : .nu. CO 2,300-3,200 cm.sup.-1 : .nu. OH
EXAMPLE 4
3-(2-(5-(1-methylpropyl)benzofuryl))-4-aminobutanoic acid
By following the instructions in stages A to D of Example 1, but replacing the 2-(5-isopropyl)benzofurylcarbaldehyde in stage A of Example 1 by 2-(5-(1-methylpropyl))benzofurylcarbaldehyde, there are obtained in succession, after a purification stage by high performance liquid chromatography, as appropriate, the following compounds:
STAGE A: ethyl 3-(2-(5-(1-methylpropyl)benzofuryl))propenoate
b.p. (0.5 mm Hg)=165.degree. C.
STAGE B: ethyl 3-(2-(5-(1-methylpropyl)benzofuryl))- 4-nitrobutanoate
STAGE C: 4-(2-(5-(1-methylpropyl)benzofuryl))-2-oxopyrrolidine
Melting point: 111.degree.-113.degree. C.
Spectral Characteristics
infrared: 1,700 cm.sup.-1 : .nu. CO 3,200 m.sup.-1 : .nu. NH
NMR (CDCl.sub.3): .delta.: 0.80 ppm: triplet: (CH.sub.3 --CH.sub.2 --) .delta.: 1.28 ppm: doublet: (CH.sub.3 --CH--) .delta.: 1.45-1.80 ppm: multiplet: (--CH.sub.2 --CH) .delta.: 2.68 ppm: doublet: (CH.sub.2 CO) .delta.: 3.50-4.10 ppm: multiplet: CH.sub.2 N, CH) .delta.: 5.66 ppm: singlet: (NH) .delta.: 6.49 ppm: singlet: (H.sub.3 ', benzofuran) .delta.: 7.00-7.50 ppm: multiplet: (H.sub.4 ', H.sub.6 ', H.sub.7 '; benzofuran)
STAGE D: 3-(2-(5-(1-methylpropyl)benzofuryl))-4-aminobutanoic acid
Melting point: 200.degree. C.
Spectral Characteristics
infrared: 1,580 cm.sup.-1 : .nu. CO 2,300-3,200 cm.sup.-1 : .nu. OH
EXAMPLE 5
3-(2-(5-fluorobenzofuryl))-4-aminobutanoic acid
By following the instructions in stages A to D of Example 1, but replacing the 2-(5-isopropyl)benzofurylcarbaldehyde in stage A of Example 1 by 2-(5-fluoro)benzofurylcarbaldehyde, there are obtained in succession, after a purification stage by high performance liquid chromatography, as appropriate, the following compounds:
STAGE A: ethyl 3-(2-(5-fluorobenzofuryl))propenoate
Melting point: 112.degree. C.
STAGE B: ethyl 3-(2-(5-fluorobenzofuryl))-4-nitrobutanoate
STAGE C: 4-(2-(5-fluorobenzofuryl))-2-oxopyrrolidine
Melting point: 178.degree.-180.degree. C.
Spectral Characteristics
infrared: 1,690 cm.sup.-1 : .nu. CO 3,200 m.sup.-1 : .nu. NH
NMR (CDCl.sub.3): .delta.: 2.70 ppm: doublet: (CH.sub.2 CO) .delta.: 3.40-4.10 ppm: multiplet: CH.sub.2 N, CH) .delta.: 5.75 ppm: singlet: (NH) .delta.: 6.50 ppm: singlet: (H.sub.3 '; benzofuran) .delta.: 6.80-7.50 ppm: multiplet: (H.sub.4 ', H.sub.6 ', H.sub.7 '; benzofuran)
STAGE D: 3-(2-(5-fluorobenzofuryl))-4-aminobutanoic acid
Melting point: 200.degree.-202.degree. C.
Spectral Characteristics
infrared: 1,580 cm.sup.-1 : .nu. CO 2,300-3,200 cm.sup.-1 : .nu. OH
NMR (D.sub.2 O): .delta.: 2.76 ppm: doublet: (CH.sub.2 CO) .delta.: 3.30-4.00 ppm: multiplet: CH.sub.2 N, CH) .delta.: 6.88 ppm: singlet: (H.sub.3 '; benzofuran) .delta.: 6.90-7.90 ppm: multiplet: (H.sub.4 ', H.sub.6 ', H.sub.7 '; benzofuran)
EXAMPLE 6
3-(2-(5-bromobenzofuryl))-4-aminobutanoic acid
By following the instructions in stages A to D of Example 1, but replacing the 2-(5-isopropyl)benzofurylcarbaldehyde in stage A of Example 1 by 2-(5-bromo)benzofurylcarbaldehyde, there are obtained in succession, after a purification stage by high performance liquid chromatography, as appropriate, the following compounds:
STAGE A: ethyl 3-(2-(5-bromobenzofuryl))propenoate
STAGE B: ethyl 3-(2-(5-bromobenzofuryl))-4-nitrobutanoate
STAGE C: 4-(2-(5-bromobenzofuryl))-2-oxopyrrolidine
STAGE D: 3-(2-(5-bromobenzofuryl))-4-aminobutanoic acid
Melting point: 200.degree.-202.degree. C.
Spectral Characteristics
infrared: 1,580 cm.sup.-1 : .nu. CO 2,300-3,200 cm.sup.-1 : .nu. OH
NMR (D.sub.2 O): .delta.: 2.76 ppm: doublet: (CH.sub.2 CO) .delta.: 3.30-4.00 ppm: multiplet: CH.sub.2 N, CH) .delta.: 6.88 ppm: singlet: (H.sub.3 '; benzofuran) .delta.: 6.90-7.90 ppm: multiplet: (H.sub.4 ', H.sub.6 ', H.sub.7 '; benzofuran)
EXAMPLE 7
3-(2-(4,5-dichlorothienyl))-4-aminobutanoic acid
By following the instructions in stages A to D of Example 1, but replacing the 2-(5-isopropyl)benzofurylcarbaldehyde in stage A of Example 1 by 2-(4,5-dichloro)thienylcarbaldehyde, there are obtained in succession, after a purification stage by high performance liquid chromatography, as appropriate, the following compounds:
STAGE A: ethyl 3-(2-(4,5-dichlorothienyl))propenoate
Melting point: 76.degree.-78.degree. C.
STAGE B: ethyl 3-(2-(4,5-dichlorothienyl))-4-nitrobutanoate
STAGE C: 4-(2-(4,5-dichlorothienyl))-2-oxopyrrolidine
STAGE D: 3-(2-(4,5-dichlorothienyl))-4-aminobutanoic acid
Melting point: 188.degree.-195.degree. C.
Spectral Characteristics;
infrared: 2,500-3,400 cm.sup.-1 : wide band (NH.sub.3.sup.+,COO--) 1,590 cm.sup.-1 : .nu. CO
NMR (D.sub.2 O): .delta.: 2.6 ppm: doublet: (CH.sub.2 CO) .delta.: 3.1-3.5 ppm: multiplet: ##STR16## .delta.: 7 ppm: singlet: (H; thiophene)
EXAMPLE 8
3-(2-imidazolyl)-4 aminobutanoic acid
By following the instructions in stages A to D of Example 1, but replacing the 2-(5-isopropyl)benzofurylcarbaldehyde in stage A of Example 1 by 2-imidazolylcarbaldehyde, there are obtained in succession, after a purification stage by high performance liquid chromatography, as appropriate, the following compounds:
STAGE A: ethyl 3-(2-imidazolyl)propenoate
STAGE B: ethyl 3-(2-imidazolyl)-4-nitrobutanoate
STAGE C: 4-(2-imidazolyl)-2-oxopyrrolidine
Spectral Characteristics
infrared 3,200-3100 cm.sup.-1 : .nu. NH 1,700 cm.sup.-1 : .nu. CO
NMR (DMSO): .delta.: 2.5 ppm: doublet: (CH.sub.2 CO) .delta.: 3.5 ppm: multiplet: ##STR17## .delta.: 6.9 ppm: singlet: (H.sub.2 '; imidazole) .delta.: 7.5 ppm: singlet: (NH--CO) .delta.: 10-11 ppm: singlet: (NH; imidazole)
STAGE D: 3-(2-imidazolyl)-4-aminobutanoic acid
Melting point: 175.degree.-180.degree. C.
Spectral Characteristics
infrared: 2,700-3,400 cm.sup.-1 : .nu. (COO--, NH.sub.3.sup.+) 1,590 cm.sup.-1 : .nu. CO
NMR (D.sub.2 O): .delta.: 2.6 ppm: doublet: (CH.sub.2 CO) .delta.: 3.2-3.7 ppm: multiplet: (CH--CH.sub.2 --NH) .delta.: 7.05 ppm: singlet: (H.sub.2 '; imidazole)
EXAMPLE 9
3-(2 (5-chlorobenzofuryl)) 4-aminobutanoic acid
By following the instructions in stages A to C of Example 1, but replacing the 2-(5-isopropyl)benzofurylcarbaldehyde in stage A of Example 1 by 2-(5-chloro)benzofurylcarbaldehyde, the following compounds are obtained in succession:
STAGE A: ethyl 3-(2-(5-chlorobenzofuryl))propenoate
STAGE B: ethyl 3-(2-(5-chlorobenzofuryl))-4-nitrobutanoate
STAGE C: 4-(2-(5-chlorobenzofuryl))-2-oxopyrrolidine
STAGE D: 1-tert.-butoxycarbonyl-2-oxo-4-(2-(5-chlorobenzofuryl))pyrrolidine
0.01 mol of triethylamine, 0.02 mol of di-tert.-butyl dicarbonate and 0.01 mol of dimethylaminopyridine are added under a nitrogen atmosphere and at ambient temperature to 0.01 mol of the compound obtained in stage C of Example 9 in solution in 50 cm.sup.3 of methylene chloride. The reaction mixture is stirred for 7 hours and then evaporated to dryness. The residue obtained is taken up in 25 cm.sup.3 of ether and the precipitate formed is removed by filtration. The ethereal phase is washed with water, dried and recrystallised from diisopropyl ether.
Melting point: 123.degree. C.
Spectral Characteristics
infrared: 3,100 cm.sup.-1 : .nu. NH 1,800 cm.sup.-1 : .nu. CO (tert.-butoxycarbonyl) 1,690 cm.sup.-1 : .nu. CO (lactam)
NMR (CDCl.sub.3): .delta.: 1.5 ppm: singlet: (tert.-butoxycarbonyl) .delta.: 2.9 ppm: doublet: (CH.sub.2 --CO) .delta.: 4 ppm: multiplet: (CH--CH.sub.2 --N) .delta.: 6.5 ppm: singlet: (H.sub.3 '; benzofuran) .delta.: 7.15 ppm: doublet: (H.sub.6 '; benzofuran) .delta.: 7.25 ppm: doublet: (H.sub.7 '; benzofuran) .delta.: 7.5 ppm: doublet: (H.sub.4 '; benzofuran)
STAGE E: 4-tert.-butoxycarbonylamino-3-(2-(5-chlorobenzofuryl))butanoic acid
0.01 mol of a molar solution of lithium hydroxide is added at ambient temperature to 0.01 mol of the compound obtained in stage D of Example 9 in solution in tetrahydrofuran. The reaction mixture is stirred for thirty minutes, the solvent is evaporated, the residue is taken up in 25 cm.sup.3 of water and then acidified with a 10% acetic acid solution. The whole is extracted with ether, dried, filtered, evaporated and recrystallised from hexane.
M.p..degree. C.: 104.degree. C.
STAGE F: 3-(2-(5-chlorobenzofuryl))-4-aminobutanoic acid
A mixture of 0.005 mol of the compound obtained in stage E of Example 9 and 0.25 mol of trifluoroacetic acid in 100 cm.sup.3 of dichloromethane is stirred for one hour at ambient temperature and then evaporated to dryness. The residue is taken up in 25 cm.sup.3 of water and then acidified to pH=1 with a solution of 10% hydrochloric acid, taken up in from 1 to 3 cm.sup.3 of trifluoroacetic acid, and chromatographed on an ion exchange resin (DOWEX 50 WH+) using a 5% ammonium hydroxide solution as eluant. The eluant is evaporated to dryness and the residue is recrystallised from water.
M.p..degree. C.: 190.degree.-192.degree. C.
IR: 2,300-3,300 cm.sup.-1 : .nu. (COO--, NH.sub.3.sup.+) 1,590 cm.sup.-1 : .nu. CO
NMR (D.sub.2 O): .delta.: 2.75 ppm: doublet, 2H: (CH.sub.2 --COOH) .delta.: 3.25-4 ppm: multiplet--3H: (CH--CH.sub.2 --NH.sub.2) .delta.: 6.8 ppm: singlet--1H: (furan) .delta.: 7.25-7.75 ppm: multiplet--3H: (benzene)
EXAMPLE 10
3-(2-benzothienyl) 4-aminobutanoic acid
By proceeding as in Example 9, but initially using 2-benzothienylcarbaldehyde in stage A of Example 9, there are obtained:
STAGE D: 1-tert.-butoxycarbonyl-2-oxo-4-(2-benzothienyl)pyrrolidine and in
STAGE F: the title product:
M.p..degree. C.: 192.degree.-197.degree. C.
IR: 2,300-3,200 cm.sup.-1 : .nu. (COO--, NH.sub.3.sup.+) 1,575 cm.sup.-1 : .nu. CO
NMR (D.sub.2 O): .delta.: 2.7 ppm: doublet--2H: (CH.sub.2 --CO) .delta.: 3.3-3.5 ppm: multiplet--2H: (CH.sub.2 --NH.sub.2) .delta.: 3.75 ppm: multiplet--1H: (CH) .delta.: 7.25-8.10 ppm: multiplet--5H: (benzothiophene)
EXAMPLE 11
3-(2-(5 ethoxybenzofuryl)) 4 aminobutanoic acid
By proceeding as in Example 9, but initially using 2-(5-ethoxy)benzofurylcarbaldehyde in stage A of Example 9, there are obtained:
STAGE C: 4-(2-(5-ethoxybenzofuryl)-2-oxopyrrolidine
Melting point: 146.degree.-148.degree. C.
Spectral Characteristics
infrared: 1,675 cm.sup.-1 : .nu. CO 3,250 cm.sup.-1 : .nu. NH
NMR (CDCl.sub.3): .delta.: 1.35 ppm: triplet: (O--CH.sub.2 --CH.sub.3) .delta.: 2.50-2.70 ppm: doublet: (CH.sub.2, CO) .delta.: 3.50-4.10 ppm: multiplet: (CH.sub.2 N, CH, O--CH.sub.2 --CH.sub.3) .delta.: 6.15 ppm: singlet: (NH) .delta.: 6.47 ppm: singlet: (H.sub.3 '; benzofuran) .delta.: 6.85 ppm: doublet: (H.sub.4 '; benzofuran) .delta.: 7.00 ppm: doublet: (H.sub.6 '; benzofuran) .delta.: 7.30 ppm: doublet: (H.sub.7 '; benzofuran)
STAGE D: 1-tert.-butoxycarbonyl-2-oxo-4-(2-(5-ethoxybenzofuryl))pyrrolidine and in
STAGE F: the title product:
Melting point: 200.degree. C.
Spectral Characteristics
infrared: 2,300-3,200 cm.sup.-1 : .nu. (COO--, NH.sub.3.sup.+) 1,620 cm.sup.-1 : .nu. CO
NMR (D.sub.2 O): .delta.: 1.40 ppm: triplet: (CH.sub.3) .delta.: 2.60: doublet: (CH.sub.2 CO) .delta.: 3.30-3.95 ppm: multiplet: (CH--CH.sub.2 --NH.sub.2) .delta.: 4.10 ppm: quintuplet: (OCH.sub.2) .delta.: 6.75 ppm: singlet: (H.sub.3 '; benzofuran) .delta.: 7.00 ppm: doublet: (H.sub.6 '; benzofuran) .delta.: 7.20 ppm: doublet: (H.sub.4 '; benzofuran)
EXAMPLE 12
3-(2-benzothiazolyl)-4-aminobutanoic acid
By proceeding as in Example 9, but initially using 2-benzothiazolylcarbaldehyde in stage A of Example 9, there are obtained:
STAGE C: 4-(2-benzothiazolyl)-2-oxopyrrolidine
STAGE D: 1-tert.-butoxycarbonyl-2-oxo-4-(2-benzothiazolyl)pyrrolidine and in
STAGE F: the title product.
EXAMPLE 13
3-(2-(5-trifluoromethylbenzofuryl))-4-aminobutanoic acid
By proceeding as in Example 9, but initially using 2-(5-trifluoromethyl)benzofurylcarbaldehyde in stage A of Example 9, there are obtained:
STAGE C: 4-(2-(5-trifluoromethylbenzofuryl))-2-oxopyrrolidine
STAGE D: 1-tert.-butoxycarbonyl-2-oxo-4-(2-(5-trifluoromethylbenzofuryl))pyrrolidine and in
STAGE F: the title product.
EXAMPLE 14
3-(2-(4-trifluoromethylthienyl))-4-aminobutanoic acid
By proceeding as in Example 9, but initially using 2-(4-trifluoromethyl)thienylcarbaldehyde in stage A of Example 9, there are obtained:
STAGE C: 4-(2-(4-trifluoromethylthienyl))-2-oxopyrrolidin
STAGE D: 1-tert.-butoxycarbonyl-2-oxo-4-(2-(5-trifluoromethylthienyl))pyrrolidine and in
STAGE F: the title product.
EXAMPLE 15
3-dicyclopropylmethyl-4-aminobutanoic acid
By proceeding as in Example 9, but initially using 2-dicyclopropylmethylcarbaldehyde in stage A of Example 9, there are obtained:
STAGE C: 4-dicyclopropylmethyl-2-oxopyrrolidine
STAGE D: 1-tert.-butoxycarbonyl-2-oxo-4-dicyclopropylmethylpyrrolidine and in
STAGE F: the title product.
EXAMPLE 16
3-(2-imidazolyl)-4-aminobutanoic acid methyl ester
STAGE A: 3-(2-imidazolyl)-4-aminobutanoic acid chloride
0.01 mol of 3-(2-imidazolyl)-4-aminobutyric acid chloride is dissolved in 30 cm.sup.3 of methylene chloride. 0.025 mol of thionyl chloride is added and the whole is stirred at ambient temperature for 2 hours. The reaction medium is evaporated and the residue is extracted twice with chloroform after being rendered alkaline. The chloroform phases are combined and dried over calcium chloride and the chloroform is evaporated off. The residue is recrystallised.
STAGE B: 3-(2-imidazolyl)-4-aminobutanoic acid methyl ester
0.01 mol of the compound obtained in stage A of Example 16 is dissolved in 30 cm.sup.3 of pyridine. 1 cm.sup.3 of methanol is added. The whole is heated under reflux for five hours, the reaction medium is evaporated in vacuo on a water bath. The residue is dried and purified by chromatography.
EXAMPLE 17
3-(2-imidazolyl)-4-aminobutyramide
0.01 mol of the 3-(2-imidazolyl)-4-aminobutanoic acid chloride obtained in Example 12, stage A, is dissolved in 50 cm.sup.3 of chloroform. A current of ammonia is bubbled in over a period of 2 hours. The reaction medium is evaporated to dryness and the residue is recrystallised.
EXAMPLE 18
3-(2-imidazolyl)-4-amino-N propylbutyramide
0.01 mol of the compound obtained in stage A of Example 16 is dissolved in 50 cm.sup.3 of chloroform. 0.025 mol of propylamine is added and the whole is heated under reflux for 5 hours while stirring and then cooled. The reaction mixture is then evaporated to dryness and extracted twice with chloroform. The chloroform phases are combined and dried over calcium chloride and the solvent is evaporated in vacuo on a water bath. The residue is purified by chromatography.
EXAMPLE 19
3-(2-(5-isopropylbenzofuryl)) 4-aminobutanoic acid methyl ester
By proceeding as in Example 16, but replacing the 3-(2-imidazolyl)-4-aminobutyric acid in stage A of Example 16 by 3-(2-(5-isopropylbenzofuryl))-4-aminobutyric acid, the title compound is obtained.
EXAMPLE 20
3-(2-(5-isopropylbenzofuryl))-4 methylaminobutanoic acid methyl ester
0.01 mol of the compound of Example 19 is dissolved in 100 cm.sup.3 of chloroform. 0.011 mol of dimethyl sulphate is added dropwise. The whole is stirred for 3 hours and the organic phase is washed with water and dried. The organic phase is evaporated to dryness and the residue is purified by chromatography.
EXAMPLE 21
3-cyclopropylmethyl-4-aminobutanoic acid
By proceeding as in Example 1, but replacing the 2-(5-isopropyl)benzofurylcarbaldehyde in stage A of Example 1 by cyclopropylacetaldehyde, there are obtained:
in STAGE C: 4-cyclopropylmethyl-2-oxopyrrolidine, and in
STAGE D: the title compound.
EXAMPLE 22
3-(2-(4,5-dichloroimidazolyl)) 4 aminobutanoic acid
By proceeding as in Example 1, but replacing the 2-(5-isopropyl)benzofurylcarbaldehyde in stage A of Example 1 by 2-(4,5-dichloro)imidazolylcarbaldehyde there are obtained:
in STAGE C: 4-(2-(4,5-dichloroimidazolyl))-2-oxopyrrolidine, and in
STAGE D: the title compound.
EXAMPLE 23
3-(2-(4-methylimidazolyl))-4-aminobutanoic acid
By proceeding as in Example 1, but replacing the 2-(5-isopropyl)benzofurylcarbaldehyde in stage A of Example 1 by 2-(4-methyl)imidazolylcarbaldehyde there are obtained:
in STAGE C: 4-(2-(4-methylimidazolyl))-2-oxopyrrolidine, and in
STAGE D: the title compound.
EXAMPLE 24
3-(2-(4,5-dichlorofuryl)-4-aminobutanoic acid
By proceeding as in Example 1, but replacing the 2-(5-isopropyl)benzofurylcarbaldehyde in stage A of Example 1 by 2-(4,5-dichloro)furylcarbaldehyde, there are obtained:
in STAGE C: 4-(2-(4,5-dichlorofuryl))-2-oxopyrrolidine, and in
STAGE D: the title compound.
EXAMPLE 25
3-(2-(4-methoxybenzothienyl)) 4 aminobutanoic acid
By proceeding as in Example 9, but using initially in stage A of Example 9 2-(4-methoxy)benzothienylcarbaldehyde, there are obtained:
in STAGE C: 4-(2-(4-methoxybenzothienyl))-2-oxopyrrolidine,
in STAGE D: 1-tert.-butoxycarbonyl-2-oxo-4-(2-(4-methoxybenzothienyl))pyrrolidine, and in
STAGE F: the title compound.
EXAMPLE 26
3-(2-imidazolyl)-4-acetylaminobutanoic acid methyl ester
0.01 mol of the compound obtained in stage B of Example 16 is dissolved in 50 cm.sup.3 of chloroform. 0.01 mol of acetic anhydride and 0.015 mol of sodium carbonate are added. The whole is heated under reflux for five hours while stirring. The reaction mixture is then cooled, evaporated to dryness and extracted with chloroform twice. The chloroform phases are combined and dried over calcium chloride and the solvent is evaporated in vacuo on a water bath. The residue is purified by chromatography.
EXAMPLE 27
3-(2-(4-methoxyfuryl))-4 aminobutanoic acid
By proceeding as in Example 1, but using 2-(4-methoxy)furylcarbaldehyde in stage A of Example 1 instead of 2-(5-isopropyl)benzofurylcarbaldehyde, there are obtained:
in STAGE C: 4-(2-(4-methoxyfuryl))-2-oxopyrrolidine, and in
STAGE D: the title compound.
EXAMPLE 28
3-(4 pyridazinyl)-4-aminobutanoic acid
By proceeding as in Example 1, but using 4-pyridazinylcarbaldehyde in stage A of Example 1 instead of 2-(5-isopropyl)benzofurylcarbaldehyde, there are obtained:
in STAGE C: 4-(4-pyridazinyl)-2-oxopyrrolidine, and in
STAGE D: the title compound.
EXAMPLE 29
3-(2 pyrimidinyl)-4-aminobutanoic acid
By proceeding as in Example 1, but using 2-pyrimidinylcarbaldehyde in stage A of Example 1 instead of 2-(5-isopropyl)benzofurylcarbaldehyde, there are obtained:
in STAGE C: 4-(2-pyrimidinyl)-2-oxopyrrolidine, and in
STAGE D: the title compound.
EXAMPLE 30
3-(2-benzimidazolyl)-4-aminobutanoic acid
By proceeding as in Example 1, but using 2-benzimidazolylcarbaldehyde in stage A of Example 1 instead of 2-(5-isopropyl)benzofurylcarbaldehyde, there are obtained:
in STAGE C: 2-(2-benzimidazolyl)-2-oxopyrrolidine, and in
STAGE D: the title compound.
EXAMPLE 31
3-(3 azepinyl)-4-aminobutanoic acid
By proceeding as in Example 1, but using 3-azepinylcarboxaldehyde in stage A of Example 1 instead of 2-(5-isopropyl)benzofurylcarbaldehyde, there are obtained:
in STAGE C: 4-(3-azepinyl)-2-oxopyrrolidine,
and in
STAGE D: the title compound.
EXAMPLE 32
3-(3 (1,4-diazepinyl))-4-aminobutanoic acid
By proceeding as in Example 1, but using (3-(1,4-diazepinyl))carboxaldehyde in stage A of Example 1 instead of 2-(5-isopropyl)benzofurylcarbaldehyde, there are obtained:
in STAGE C: 4-(3-(1,4-diazepinyl))-2-oxopyrrolidine, and in
STAGE D: the title compound.
EXAMPLE 33
3-(3-benzo[b]azepinyl)-4 aminobutanoic acid
By proceeding as in Example 1, but using (3-benzo[b]azepinylcarbaldehyde in stage A of Example 1 instead of 2-(5-isopropyl)benzofurylcarbaldehyde, there are obtained:
in STAGE C: 4-(3-benzo[b]azepinyl)-2-oxopyrrolidine, and in
STAGE D: the title compound.
EXAMPLE 34
3-(2-(1,3,5-triazinyl))-4-aminobutanoic acid
By proceeding as in Example 1, but using (2-(1,3,5-triazinyl))carbaldehyde in stage A of Example 1 instead of 2-(5-isopropyl)benzofurylcarbaldehyde, there are obtained:
in STAGE C: 4-(2-(1,3,5-triazinyl))-2-oxopyrrolidine, and in
STAGE D: the title compound.
EXAMPLE 35
3-(2-indolyl)-4-aminobutanoic acid
By proceeding as in Example 9, but using initially in stage A of Example 9 2-indolylcarbaldehyde, there are obtained:
STAGE C: 4-(2-indolyl)-2-oxopyrrolidine,
STAGE D: 1-tert.-butoxycarbonyl-2-oxo-4-(2-indolyl)pyrrolidine, and in
STAGE F: the title compound.
EXAMPLE 36
3-(2 (5-methoxyindolyl)) 4-aminobutanoic acid
By proceeding as in Example 9, but using initially in stage A of Example 9 2-(5-methoxy)indolylcarbaldehyde, there are obtained:
STAGE C: 4-(2-(5-methoxyindolyl))-2-oxopyrrolidine,
STAGE D: 1-tert.-butoxycarbonyl-2-oxo-4-(2-(5-methoxyindolyl))pyrrolidine, and in
STAGE F: the title compound.
EXAMPLE 37
3-(2-(5-methylindolyl))-4-aminobutanoic acid
By proceeding as in Example 9, but using initially in stage A of Example 9 2-(5-methyl)indolylcarbaldehyde, there are obtained:
STAGE C: 4-(2-(5-methylindolyl))-2-oxopyrrolidine,
STAGE D: 1-tert.-butoxycarbonyl-2-oxo-4-(2-(5-methylindolyl))pyrrolidine, and in
STAGE F: the title compound.
EXAMPLE 38
3-(2-(5-chloroindolyl))-4 aminobutanoic acid
By proceeding as in Example 9, but using initially in stage A of Example 9 2-(5-chloro)indolylcarbaldehyde, there are obtained:
STAGE C: 4-(2-(5-chloroindolyl))-2-oxopyrrolidine,
STAGE D: 1- tert.- butoxycarbony 1-2-oxo-4- (3-(5-chloroindolyl))pyrrolidine, and in
STAGE F: the title compound.
EXAMPLE 39
4-tert.-butoxycarbonylamino 3 (2-(5-methoxybenzofuryl))butanoic acid
By proceeding as in Example 9, but initially using 2-(5-methoxy)benzofurylcarbaldehyde in Stage A of Example 9, there is obtained:
in STAGE D: 1-tert.-butoxycarbonyl-2-oxo-4-(2-(5-methoxybenzofuryl))pyrrolidine
Melting point: 90.degree.-92.degree. C.
Spectral Characteristics
infrared: 1710-1740 cm.sup.-1 : .nu. CO NMR (CDCl.sub.3): .delta.: 1.53 ppm: singlet: (C(CH.sub.3).sub.3) .delta.: 2.87 ppm: doublet: (CH.sub.2 CO) .delta.: 3.83 ppm: multiplet: (CH.sub.3 O, CH, CH.sub.2 N) .delta.: 6.48 ppm: singlet: (H.sub.3 '; benzofuran) .delta.: 6.87 ppm: doublet: (H.sub.6 '; benzofuran) .delta.: 7.00 ppm: doublet: (H.sub.4 '; benzofuran) .delta.: 7.44 ppm: doublet: (H.sub.7 '; benzofuran) and in
STAGE E: the title product:
Melting point: 149.degree.-153.degree. C.
Spectral Characteristics
infrared: 1,700-1,720 cm.sup.-1 : .nu. CO 3,420 cm.sup.-1 : .nu. NH
NMR (CDCl.sub.3): .delta.: 1.40 ppm: singlet: (C(CH.sub.3).sub.3) .delta.: 2.70-2.90 ppm: multiplet: (CH.sub.2 CO) .delta.: 3.40-3.70 ppm: multiplet: CH.sub.2 N, CH) .delta.: 3.82 ppm: singlet: (CH.sub.3 O) .delta.: 4.70 ppm: singlet: (NH) .delta.: 6 45 ppm: singlet: (H.sub.3 '; benzofuran) .delta.: 6.82 ppm: doublet: (H.sub.6 '; benzofuran) .delta.: 6.95 ppm: doublet: (H.sub.4 '; benzofuran) .delta.: 7.33 ppm: doublet: (H.sub.7 '; benzofuran)
EXAMPLE 40
4-tert.-butoxycarbonylamino-3-(2-(5-methoxyoenzofuryl))butanamide
STAGE A: 4-tert.-butoxycarbonylamino-3-(2-(5-methoxybenzofuryl))butanamide acid chloride
By proceeding as in stage A of Example 16, but replacing the 3-(2-imidazolyl)-4-aminobutyric acid by the compound of Example 39, the product of stage A is obtained.
STAGE B: 4-tert.-butoxycarbonylamino-3-(2-(5-methoxybenzofuryl))butanamide
By proceeding as in Example 17, but replacing the 3-(2-imidazolyl)-4-aminobutanoic acid chloride by the compound obtained in stage A of Example 40, the title product is obtained: Melting point: 175.degree.-177.degree. C. Spectral Characteristics
infrared: 1,665-1,700 cm.sup.-1 : .nu. CO 3,400 cm.sup.-1 : .nu. NH
PHARMACOLOGICAL STUDY OF THE COMPOUNDS OF THE INVENTION
EXAMPLE 41
Acute Toxicity Study
Acute toxicity was assessed after the oral administration to groups of 5 mice (20.+-.2 grams) of increasing doses (0.05; 0.1; 0.25; 0.50; 0.75 g/kg). The animals were observed at regular intervals over the course of the first day and daily for the two weeks following treatment.
It appears that the compounds of the invention are atoxic.
EXAMPLE 42
Study of the Affinity to GABA.sub.B Receptors
This study was carried out in accordance with conventional binding study techniques.
It appears that the compounds of the invention have a very strong affinity to GABA.sub.B receptors.
Thus, some of the compounds of the invention have an IC.sub.50 of 0.05 .mu.M in the presence of R(-) [.sup.3 H] baclofen. By way of comparison, the best compound of the prior art having a similar structure (J. Med. Chem. 1987, 30, 743-746) had an IC.sub.50 in this test of 0.61 .mu.M and baclofen had an IC.sub.50 of 0.33 .mu.M.
EXAMPLE 43
Study of the Inhibition of Excitation Activity Induced by Convulsants
The compounds of the invention antagonise the excitatory activity induced by convulsants in hippocampal preparations.
Hippocampal preparations were prepared from the brains of sacrificed adult female rats. Transverse sections were placed in a preservative medium. After 1 hour and 30 minutes' incubation, the isolated preparations were placed under registration, perfused with a saline solution and oxygenated. Spontaneous potentials appear which are associated with CA3 pyramidal cells. Excitation is induced by the addition of bicucullin (50 .mu.M).
The compounds of the invention, at various concentrations, or baclofen are then added.
The baclofen or the compounds of the invention are perfused for periods of 10 minutes. The excitation rate is calculated during the four minutes preceding the perfusion of baclofen or the compounds of the invention and during the last four minutes of the perfusion of the products to be studied.
The inhibition is expressed as percentage excitation in relation to the initial rate, which permits the determination of an inhibiting concentration 50 (IC.sub.50). The compounds of the invention have an IC.sub.50 of from 5 to 20 .mu.M; baclofen in this test has an IC.sub.50 of approximately 50 .mu.M.
EXAMPLE 44
Stimulation of the Synthesis of Cyclic AMP in the Brain
The compounds to be tested are administered intraperitoneally at a dose of 10 mg/kg to mice of the OF1/IFFA Credo strain.
24 hours after the last injection, the animals are sacrificed by freezing, the cAMP present in these cerebral structures is dosed by radioimmunology according to Amersham's method (specific binding protein). Some of the compounds of the invention appear to be capable of greatly increasing the cerebral synthesis of cyclic AMP.
EXAMPLE 45
Pharmaceutical Composition: Tablets
Tablets each containing 1.5 mg of 3-(2-imidazolyl) -4-aminobutanoic acid.
Formula for 1,000 tablets:
______________________________________3-(2-imidazolyl)-4-aminobutanoic acid 1.5 gwheat starch 15 gcornstarch 15 glactose 90 gmagnesium stearate 2 gsilica 1 ghydroxypropylcellulose 2 g______________________________________

Number	Name	Date
3316260	Shen et al.	Apr 1967
3573304	Eberle et al.	Mar 1971
4579862	Manley et al.	Apr 1986
4636518	Fellner et al.	Jan 1987

4-aminobutyric acid compounds, compositions and methods of use for treating disorders related to a dysfunction of GABA.sub.B receptors

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Poster identified as Document A presented at Cambridge in 1989.
Berthelot et al., J. Med. Chem., vol. 30, No. 4, pp. 743-746 (1987).
Chemical Abstracts, vol. 106, entry 176087e, 1987.
Chemical Abstracts, vol. 111, entry 50294e, 1989.
Chemical Abstracts, vol. 111, entry 50265w, 1989.