4,5,6-substituted-N-(substituted-phenyl)-2-pyrimidinamines

BRIEF SUMMARY OF THE INVENTION
This invention relates to new organic compounds and, more particularly, is concerned with novel 4,5,6-substituted-N-(substituted-phenyl)-2-pyrimidinamines having anti-asthmatic activity which may be represented by the following structural formula: ##STR1## wherein R.sub.1 is hydrogen, alkyl(C.sub.1 -C.sub.3), --COCO.sub.2 C.sub.2 H.sub.5 or N,N-dimethylaminoethyl; R.sub.2 is mono- or poly-substituted phenyl wherein the substituents are alkyl(C.sub.1 -C.sub.6), alkoxy(C.sub.1 -C.sub.3), chloro, bromo, iodo, trifluoromethyl, hydroxy, phenyl, amino, monoalkyl-(C.sub.1 -C.sub.3)amino, dialkyl(C.sub.1 -C.sub.3)amino, alkyl(C.sub.1 -C.sub.3)keto, propenyloxy, carboxyl, oxyacetic acid, oxyacetic acid ethyl ester, sulfamilamido, N,N-dialkyl(C.sub.1 -C.sub.3)sulfamilamido, N-methylpiperazinyl, piperidinyl, 1H-imidazol-1-yl, 1H-triazol-1-yl, 1H-benzimidazol-z-yl, 1-naphthyl, cyclopentyl, 3,4-dimethylbenzyl or moieties of the formula: ##STR2## wherein R is alkyl(C.sub.1 -C.sub.3), X is oxygen (--O--) or sulfur (--S--), m is 1-3, n is 2 or 3, R.sub.6 is hydrogen, alkyl(C.sub.1 -C.sub.3), alkoxy (C.sub.1 -C.sub.3), chloro, bromo, iodo or trifluoromethyl, R.sub.7 is 1H-imidazol-1-yl or morpholino and R.sub.8 is alkyl(C.sub.1 -C.sub.3), phenyl or monosubstituted phenyl wherein the substituents are alkyl(C.sub.1 -C.sub.3), halogen or trifluoromethyl; R.sub.3 is 2-pyridinyl, 3-pyridinyl, 4-pyridinyl, 2-methyl-3-pyridinyl, 4-methyl-3-pyridinyl, 2-furanyl, 5-methyl-2-furanyl, 2,5-dimethyl-3-furanyl, 2-thienyl, 3-thienyl, 5-methyl-2-thienyl, 2-pheno-thiazinyl, 4-pyrazinyl, 2-benzofuranyl, 2-(pyridine-N-oxide), 3-(pyridine-N-oxide), 4-(pyridine-N-oxide), 1H-indol-2-yl, 1H-indol-3-yl, 1-methyl-1H-pyrrol-2-yl, 4-quinolinyl, 4-pyri-dinyl methyl iodide, dimethylaminophenyl or N-acetyl-N-methylaminophenyl; R.sub.4 is hydrogen or alkyl(C.sub.1 -C.sub.3); and R.sub.5 is hydrogen or alkyl(C.sub.1 -C.sub.3); and the pharmacologically acceptable acid-addition salts thereof.
The present invention also icludes novel compositions of matter containing the above-defined compounds which are useful for treating asthma, allergic diseases, inflammation and diabetes in mammals. The invention also comprises processes of preparing the compounds within the scope of the above formula.
DETAILED DESCRIPTION OF THE INVENTION
The novel compounds of the present invention are obtainable as crystalline materials having characteristic melting points and absorption spectra. They are in general sparingly soluble in organic solvents such as lower alkanols, chloroform, tetrahydrofuran, N,N-dimethylformamide, dichloromethane, acetone and the like, but are generally insoluble in water.
The novel 4,5,6-substituted-2-pyrimidinamines of the present invention in general may be prepared as set forth in the following reaction schemes. ##STR3## wherein R.sub.1, R.sub.2, R.sub.3, R.sub.4 and R.sub.5 are as hereinabove defined.
In accordance with Scheme I, a heteroaryl (R.sub.3) alkanoyl (R.sub.4) compound 1, e.g 2-acetylpyridine, 2-acetylfuran, 3-acetylthiophene, 2-acetyl-6-methylpyridine, 2-propionyl pyridine or 3-propionyl pyridine and the like, is reacted with a di(lower alkyl)-formamide or acetamide di(lower alkyl) acetal 2, e.g; N,N-dimethylformamide dimethylacetal or N,N-dimethylacetamide dimethylacetal at an elevated temperature in the range of about 50.degree. C. to about 150.degree. C. for from about 4 to 24 hours to produce the 3-di(lower alkyl)aminoacrylophenone 3. The acrylophenone 3 is then reacted with an appropriately substituted phenylguanidine (R.sub.1)(R.sub.2), 4 as the base or as the carbonate, sulfate, nitrate, hydrochloride or dihydrochloride salt in an inert solvent such as absolute ethanol, n-propanol, isopropyl alcohol or 2-methoxyethanol and the like, by heating at the reflux temperature for from 6-48 hours. The product 5 is separated by the partial evaporation of the solvent, then cooling and collected and recrystallized in a conventional manner from solvents such as n-propyl alcohol, isopropyl alcohol, absolute ethyl alcohol or 2-methoxyethanol and the like and combinations of solvents such as chloroform/hexane, dichloromethane/hexane or isopropyl alcohol/ethylene glycol monomethyl ether and the like. ##STR4## wherein R.sub.1, R.sub.2, R.sub.3, R.sub.4 and R.sub.5 are as hereinabove defined.
In accordance with Scheme II, when the 4,5,6-substituted-2-pyrimidinamine product 5 is dissolved by heating in a solvent such as absolute ethanol, isopropyl alcohol or dichloromethane, then stirred at room temperature and reacted with a mineral acid such as sulfuric acid, hydrochloric acid, nitric acid or phosphoric acid and the like, dissolved in absolute ethanol or isopropyl alcohol and the like, the 4,5,6-substituted-2-pyrimidinamine acid addition salt 6 is precipitated on standing for 30 minutes and chilling for several hours.
Alternatively, acid addition salts may be formed with organic acids such as citric acid or maleic acid and the like by dissolving the desired 4,5,6-substituted-2-pyrimidinamine in hot, absolute ethanol or 2-methoxyethanol in the presence of the organic acid. Cooling provides the desired compounds as solids.
The novel compounds of the present invention are highly active as antiasthmatic and antiallergic agents as will be demonstrated hereinbelow.
The bronchospasm of allergic asthma is a consequence of the release of mediators, such as histamine and slow-reacting substances from masts cells. The role of mediator release in the induction of an asthmatic attack has been fully reviewed and documented; see Kaliner, M. and Austen, K. F., Bronchial Asthma Mechanisms and Therepautics, E. B. Weiss, Editor, Little, Brown and Company, Boston, 163, (1976); Lichtenstein, L. M., Asthma-Physiology, Immunopharmacology and Treatment, Second International Symposium, L. M. Lichtenstein and K. F. Austen, Editors, Academic Press, New York, 51, (1979); and Bell, S. C., et al., Annular Reports in Medicinal Chemistry, 14, 51, H. J. Hess, Editor, Academic Press, New York, (1979).
The novel compounds of this invention have been tested by the procedure of Lichtenstein, L. M. and Osler, A. G., J. Exp. Med., 120, 507-530 (1964), which evaluates the ability of compounds to inhibit mediator (histamine) release from immunologically stimulated human basophils.
Reagents
10X Concentrated Tris Buffer
Dissolve 140.3 g of sodium chloride, 7.45 g of Trizma-Tris Pre-Set, Reagent Grade, pH 7.6, at 25.degree. C. (Sigma Chemical Co.) in sufficient water to give a final volume of 2 liters.
Human Albumin
(Sigma Chemical Co.) (30 mg/ml)
Calcium and Magnesium Stocks
Made to 0.075M 0.5M respectively, with calcium chloride dihydrate and magnesium chloride hexahydrate.
Tris-A Buffer
A 10 ml portion of 10X Tris Buffer and 1.0 ml of human albumin are diluted to 100 ml with water.
Tris ACM Buffer
A 10 ml portion of 10X Tris Buffer, 1.0 ml of human albumin, 0.8 ml of calcium stock and 0.2 ml of magnesium stock are diluted to 100 ml with water.
Rabbit Antihuman IgE
Behring Diagnostics (Generally used at 10 .mu.g protein/ml final concentration).
House Dust Mite Extract (Dermatophagoides Farinae)
Strength 1:100 (w:v) allergenic extract, Hollister-Stier Labs. Generally this is diluted 1:1000 to 1:10,000 (considering the vial as stock).
Other Allergens
Interdermal solutions or intramuscular preparations for hyposensitization, Hollister-Steir Labs. The final concentration used is on the order of 1 PNU/ml.
Separation of Leukocytes from Human Blood and Challenge
Eighty millimeters of blood is withdrawn from subjects with known histamine release to anti-IgE, ragweed antigen or other specific allergen, using four 20 ml heparinized tubes. This 80 ml of blood is mixed with 20 ml of saline containing 0.6 g of dextrose and 1.2 g of dextran. The blood is allowed to sediment at room temperature in two 50 ml polycarbonate centrifuge tubes until a sharp interface develops between the red cells and plasma (60-90 minutes). The plasma (top) layer from each tube is withdrawn by pipet and transferred to respective 50 ml polycarbonate tubes. The plasma is centrifuged for 8 minutes at 110X G at 4.degree. C. The supernatant is carefully poured off as completely as possible and the cell button is resuspended in 2-3 ml of Tris-A buffer using a siliconized Pasteur pipet. The resuspension is accomplished by drawing the liquid gently in an out of the pipet, with the tip below the liquid until an even suspension of cells is obtained. Sufficient Tris-A buffer is then added to bring the volume in the tube to about 45 ml and the tube is centrifuged at 110X G for 8 minutes at 4.degree. C. The supernatant is poured off and the cell button is resuspended and centrifuged as described above. The supernatant is poured off and the cell button is suspended in 2-3 ml of Tris-ACM buffer to make the final volume sufficient to allow addition to the reaction tubes.
Reaction tubes containing anti-IgE or antigens, either alone or with test compound in a total volume of 0.2 ml are prepared and placed in a 37.degree. C. bath. The cells are warmed to 37.degree. C. and frequently swirled to ensure an even suspension, while 1.0 ml aliquots are added to each reaction tube. The tubes are then incubated for 60 minutes at 37.degree. C., vortexing the tubes gently every 15 minutes to keep the cells evenly suspended. When the reaction is complete, the tubes are centrifuged at 4.degree. C. for 10 minutes at 1500 rpm to sediment the cells. One ml aliquots of supernatant are transferred to 12 mm by 75 mm polyethylene tubes and 0.2 ml of 8% perchloric acid is added to each tube. Blanks and totals are included in each test. The blanks have cells and all reagents except antigen or anti-IgE. The totals contain 0.24 ml of 8% perchloric acid, one ml of cells and 0.2 ml of buffer. All samples are then centrifuged to remove the precipitate protein.
Assay of Released Histamine by the Automated Fluorometric Method
This automated method has been described by Siraganian, R. P., in Anal. Biochem. 57, 383 (1974) and J. Immunol. Methods, 7, 283 (1975) and is based on the manual method of Shore, P. A., et al., J. Pharmacol. Exp. Ther., 217, 182 (1959).
The automated system consists of the following Technicon Autoanalyzer II components: Sampler IV, Dual-Speed Proportioning Pump III, Fluoronephelometer with a narrow pass primary filter 7-60 and a secondary filter 3-74, Recorder, and Digital Printer. The manifold used is the one described by Siraganian vide supra, with the following modifications: the dialyzer is omitted; all pumping tubes pass through a single proportioning pump with large capacity and twice the volume of sample is taken for analysis.
The automated chemistry consists of the following steps: Extraction from alkaline saline into butanol, back extraction into dilute hydrochloric acid by addition of heptane, reaction of histamine with o-phthaldialdehyde (OPT) at high pH and conversion of the OPT adduct to a stable fluorophore with phosphoric acid. The reaction product is then passed through the fluorometer. The full scale response is adjusted to 50 ng histamine base with a threshold sensitivity of approximately 0.5 ng.
Calculation of the Results of Histamine Release Tests
The instrument blank (wash) is subtracted from the ng histamine of each sample. Then the ng histamine of each sample is divided by the mean of the three totals (cells lysed with perchloric acid) to obtain percent release.
Control samples contain antigen but no test compound. Blank (or spontaneous release) samples contain neither antigen nor test compound. The mean of the blanks (three replicates) is subtracted from the percent release for controls and test compounds.
The means for control and test compound groups are computed and the result for a test compound is computed as percent of control by the formula: ##EQU1##
Values obtained at different concentrations of test compound are used to calculate an IC.sub.50 (the concentration in .mu.M which causes a 50% inhibition of histamine release) by linear regression. A compound is considered active if the IC.sub.50 is .ltoreq.48 .mu.M.
The results of this test on typical compounds of this invention appear in Table I.
TABLE I______________________________________Inhibition of Histamine Release fromImmunologically Stimulated Human BasophilsCompound IC.sub.50 (.mu.M)______________________________________4-(2-Furanyl)-5-methyl- .sub.--N--phenyl-2-pyrimidin- 17.7amine4-(4-Pyridinyl)- .sub.--N--[(3-trifluoromethyl)phenyl]- 32.02-pyrimidinamine .sub.--N--(4-Methoxyphenyl)-4-(3-pyridinyl)-2- 1.4pyrimidinamine .sub.--N--Phenyl-4-(3-pyridinyl)-2-pyrimidinamine 0.9 .sub.--N--(4-Acetylphenyl)-4-(3-pyridinyl)-2- 0.8pyrimidinamine .sub.--N--(4-Fluorophenyl)-4-(3-pyridinyl)-2- <48pyrimidinamine .sub.--N--(4-Methoxyphenyl)-4-(2-pyridinyl)-2-pyrimi- 8.3dinamine .sub.--N--(4-Methoxyphenyl)-4-(4-pyridinyl)-2-pyrimi- 1.0dinamine .sub.--N--(4-Fluorophenyl)-4-(4-pyridinyl)-2-pyrimi- 1.9dinamine .sub.--N--(4-Bromophenyl)-4-(3-pyridinyl)-2-pyrimi- 2.3dinamine4-(3-Pyridinyl)- .sub.--N--[3-(trifluoromethyl)phenyl]- 0.72-pyrimidinamine, hydrochloride4-(2-Pyridinyl)- .sub.--N--[3-(trifluoromethyl)phenyl]- 2.92-pyrimidinamine .sub.--N--(4-Methoxyphenyl)-4-(2-thienyl)-2-pyrimi- 3.9dinamine .sub.--N--(4-Ethylphenyl)-4-(1-methyl-1 .sub.--H--pyrrol-2- <48yl)-2-pyrimidinamine .sub.--N--Phenyl-4-(2-thienyl)-2-pyrimidinamine 31.7 .sub.--N--(3-Chloro-4-methylphenyl)-4-(3-pyridinyl)-2- 9.3pyrimidinamine .sub.--N--(3-Methylphenyl)-4-(2-pyridinyl)-2-pyrimi- 0.7dinamine .sub.--N--(3-Methylphenyl)-4-(4-pyridinyl)-2-pyrimi- 9.4dinamine .sub.--N--Phenyl-4-(4-pyridinyl)-2-pyrimidinamine 0.9 .sub.--N--(3-Methylphenyl)-4-(3-pyridinyl)-2-pyrimi- 1.5dinamine .sub.--N--(4-Ethylphenyl)-4-(4-pyridinyl)-2-pyrimi- 7.7dinamine .sub.--N--(4-Ethylphenyl)-5-methyl-4-(4-pyridinyl)-2- <48pyrimidinamine .sub.--N--(4-Ethylphenyl)-4-(3-pyridinyl)-2-pyrimi- <48dinamine .sub.--N--(4-Ethylphenyl)-4-(2-pyridinyl)-2-pyrimi- 2.1dinamine .sub.--N--(3-Methylphenyl)-4-(2-thienyl)-2-pyrimi- 0.3dinamine4-(2-Furanyl)- .sub.--N--phenyl-2-pyrimidinamine 484-(2-Furanyl)- .sub.--N--(3-methylphenyl)-2-pyrimi- 3.5dinamine .sub.--N--(4-Ethylphenyl)-4-(6-methyl-3-pyridinyl)-2- 13.4pyrimidinamine .sub.--N--(4-Ethylphenyl)-6-methyl-4-(6-methyl-3- 19.1pyridinyl)-2-pyrimidinamine .sub.--N--[4-(4-Methyl-1-piperazinyl)phenyl]-4-(2- <24thienyl)-2-pyrimidinamine .sub.--N--(4-Ethylphenyl)-4-pyrazinyl-2-pyrimidinamine 2.8 .sub.--N--(3-Methylphenyl)-4-pyrazinyl-2-pyrimi- 5.4dinamine .sub.--N--(2-Methylphenyl)-4-(4-pyridinyl)-2-pyrimi- 3.9dinamine .sub.--N--(3-Ethylphenyl)-4-(4-pyridinyl)-2-pyrimi- 10.6dinamine .sub.--N--(2,5-Dimethylphenyl)-4-(4-pyridinyl)-2- 47.1pyrimidinamine .sub.--N--(2,3-Dimethylphenyl)-4-(4-pyridinyl)-2- 20.2pyrimidinamine .sub.--N--(3-Methylphenyl)-4-(3-thienyl)-2-pyrimidin- 3.8amine .sub.--N--(2,5-Dimethylphenyl)-4-(2-pyridinyl)-2- <48pyrimidinamine .sub.--N--(3,5-Dimethylphenyl)-4-(4-pyridinyl)-2- 4.4pyrimidinamine .sub.--N--1-Naphthalenyl-4-(4-pyridinyl)-2-pyrimidin- 31.3amine .sub.--N--(3,5-Dimethylphenyl)-4-(2-pyridinyl)-2- 1.0pyrimidinamine .sub.--N--1-Naphthalenyl-4-(2-pyridinyl-2-pyrimidin- 3.0amine .sub.--N--(2,4-Dimethylphenyl)-4-(4-pyridinyl)-2- 24.0pyrimidinamine4-(4-Pyridinyl)- .sub.--N--(2,4,6-trimethylphenyl)-2- 10.5pyrimidinamine4-(2-Furanyl)- .sub.--N--(4-methoxyphenyl)-2-pyrimidin- <48amine .sub.--N--[4-(4-Methyl-1-piperazinyl)phenyl]-4-(2- <24pyridinyl)-2-pyrimidinamine4-(2-Furanyl)- .sub.--N--[3-(trifluoromethyl)phenyl]-2- <48pyrimidinamine .sub.--N--(4-Fluorophenyl)-4-(2-furanyl)-2-pyrimidin- 13.3amine .sub.--N--Cyclopentyl-4-(2-pyridinyl)-2-pyrimidinamine 2.2 .sub.--N--Phenyl-4-(4-pyridinyl)-2-pyrimidinamine, 3.5compound with 2-hydroxy-1,2,3-propanetricar-boxylate (2:1) .sub.--N--Phenyl-4-(4-pyridinyl)-2-pyrimidinamine, 1.0(Z)-2-butenedioate (1:1) .sub.--N--Phenyl-4-(4-pyridinyl)-2-pyrimidinamine, 3.0sulfate .sub.--N--Phenyl-4-(4-pyridinyl)-2-pyrimidinamine, 1.2dinitrate .sub.--N--(4-Ethylphenyl)-4-(4-pyridinyl)-2-pyrimidin- 17.7amine, pyridine-1-oxide .sub.--N--(3,4-Dimethylphenyl)-4-(2-pyridinyl)-2- 5.9pyrimidinamine .sub.--N--(4-Methoxyphenyl)-4-(3-thienyl)-2-pyrimidin- 15.6amine .sub.--N--(3-Ethylphenyl)-4-(2-furanyl)-2-pyrimidin- 9.7amine4-(1 .sub.--H--Indol-3-yl)- .sub.--N--phenyl-2-pyrimidinamine 3.0 .sub.--N--(2-Methoxy-5-methylphenyl)-4-(4-pyridinyl)- 6.92-pyrimidinamine .sub.--N--(3-Methylphenyl)-4-(1-methyl-1 .sub.-- H--pyrrol-2- 9.4yl)-2-pyrimidinamine .sub.--N--(3-Ethylphenyl)-4-(2-thienyl)-2-pyrimidin- 48.0amine .sub.--N--(3-Ethylphenyl)-4-(3-thienyl)-2-pyrimidin- 1.1amine4-(1 .sub.--H--Indol-2-yl)- .sub.--N--(3-methylphenyl)-2-pyrimi- 2.2dinamine4-[[4-(4-Pyridinyl)-2-pyrimidinyl]amino]- 27.5benzoic acid, methyl ester .sub.--N--(3-Methylphenyl)-4-(4-quinolinyl)-2-pyrimi- 10.9dinamine .sub.--N--Phenyl-4-(-4-quinolinyl)-2-pyrimidinamine 3.0 .sub.--N--(4-Ethylphenyl)-4-(4-quinolinyl)-2-pyrimi- 4.0dinamine4-(2-Pyridinyl)- .sub.--N--[3-(trifluoromethyl)phenyl]- 3.02-pyrimidinamine, sulfate .sub.--N--(3-Methylphenyl)-4-(2-thienyl)-2-pyrimidin- 3.0amine, sulfate4-(2-Furanyl)- .sub.--N--[3-(methylphenyl)]-2-pyrimi- 3.0dinamine, sulfate .sub.--N--Phenyl-4-(4-pyridinyl)-2-pyrimidinamine, 3.3phosphate .sub.--N--(3,5-Dimethylphenyl)-4-(2-furanyl)-2-pyrimi- 0.7dinamine .sub.--N--(3,5-Dimethylphenyl)-4-(2-thienyl)-2-pyrimi- 4.3dinamine .sub.--N--(2,4-Difluorophenyl)-4-(4-pridinyl)-2- <48pyrimidinamine .sub.--N--(2,4-Difluorophenyl)-4-(3-pyridinyl)-2- <48pyrimidinamine .sub.--N--(3-Methylphenyl)-4-(5-methyl-2-thienyl)- 1.42-pyrimidinamine .sub.--N--(2,6-Difluorophenyl)-4-(4-pyridinyl)-2- 2.9pyrimidinamine4-(4-Pyridinyl)- .sub.--N--[3-(trifluoromethyl)phenyl]- <482-pyrimidinamine, sulfate .sub.--N--(4-Methoxyphenyl)-4-(3-pyridinyl)-2-pyrimi- <48dinamine, sulfate .sub. --N--Phenyl-4-(3-pyridinyl)-2-pyrimidinamine, 3.0sulfate4-(3-Pyridinyl)- .sub.--N--[3-(trifluoromethyl)phenyl]- 2.62-pyrimidinamine, sulfate .sub.--N--Phenyl-4-(4-pyridinyl)-2-pyrimidinamine, 3.0dihydrochloride .sub.--N--[4-(1,1-Dimethylethyl)phenyl]-4-(3-pyridin- 0.7yl)-2-pyrimidinamine .sub.--N--(2,6-Difluorophenyl)-4-(3-pyridinyl)-2- 22.0pyrimidinamine .sub.--N--(4-Ethylphenyl)-4-(5-methyl-2-thienyl)-2- 36.3pyrimidinamine .sub.--N--[(3,4-Dimethylphenyl)methyl]-4-(2-pyridinyl- 39.82-pyrimidinamine .sub.--N--(3,5-Dimethylphenyl)-4-(3-pyridinyl)-2- 3.0pyrimidinamine, sulfate .sub.--N--(3,5-Dimethylphenyl)-4-(3-pyridinyl)-2- 3.0pyrimidinamine, phosphate .sub.--N--(3-Methylphenyl)-4-(1 .sub.--H--pyrrol-2-yl)-2- 11.1pyrimidinamine4-(5-Methyl-2-furanyl)- .sub.--N--(3-methylphenyl)-2- 2.0pyrimidinamine4-Methyl-6-(5-methyl-2-thienyl)- .sub.--N--phenyl-2- 24.8pyrimidinamine .sub.--N--[4-(Dimethylamino)phenyl]-4-(4-pyridinyl)-2- 3.8pyrimidinamine .sub.--N--(3-Methoxyphenyl)-4-(4-pyridinyl)-2-pyrimi- 0.4dinamine .sub.--N--(3-Methoxyphenyl)-4-(2-pyridinyl)-2-pyrimi- 0.2dinamine .sub.--N--[4-(Dimethylamino)phenyl]-4-(2-pyridinyl)-2- 2.7pyrimidinamine .sub.--N--(3-Methoxyphenyl)-4-(3-pyridinyl)-2-pyrimi- 0.3dinamine .sub.--N--(3,5-Dimethylphenyl)-4-(3-pyridinyl)-2- 0.8pyrimidinamine4-[[4-(3-Pyridinyl)-2-pyrimidinyl]amino]- 12.4benzoic acid, ethyl ester .sub.--N, .sub.--N--Dimethyl- .sub.--N'-- [4-(3-pyridinyl)-2-pyrimidin- 3.7yl]-1,4-benzenediamine4-(2,5-Dimethyl-3-furanyl)- .sub.--N--phenyl-2-pyrimi- 2.0dinamine .sub.--N, .sub.--N--Dimethyl- .sub.--N'-- [4-(4-pyridinyl)-2-pyrimi- 0.4dinyl)benzenediamine, trihydrochloride4-(2,5-Dimethyl-3-furanyl)- .sub.--N--(3-methylphenyl)- 28.52-pyrimidinamine4-(2,5-Dimethyl-3-furanyl)- .sub.--N--(3,5-dimethyl- 4.1phenyl-2-pyrimidinamine .sub.--N, .sub.--N--Dimethyl- .sub.--N'--[4-(2-pyridinyl)-2-pyrimidin- 4.4ly]-1,4-benzenediamine, dihydrochloride4-(2,5-Dimethyl-3-furanyl)- .sub.--N--(4-ethylphenyl)- 19.22-pyrimidinamine .sub.--N, .sub.--N--Dimethyl- .sub.--N'--[4-(3-pyridinyl)-2-pyrimi- 1.7dinyl]-1,3-benzenediamine3-[[4-(2-Pyridinyl)-2-pyrimidinyl]amino]ben- 3.0zoic acid, ethyl ester .sub.--N, .sub.--N--Dimethyl- .sub.--N'--[4-(2-pyridinyl)-2-pyrimidin- 0.5yl]-1,3-benzenediamine4-[[4-(3-Pyridinyl)-2-pyrimidinyl]amino]phenol 5.13-[[4-(3-Pyridinyl)-2-pyrimidinyl]amino]ben- 20.3zoic acid, ethyl ester .sub.--N--(4-Methoxyphenyl)-4-(3-pyridinyl)-2-pyrimi- 3.2dinamine, phosphate .sub.--N--(3,5-Dimethylphenyl)-4-(2-pyridinyl)-2- 0.6pyrimidinamine, sulfate .sub. --N--[4-(2-Propenyloxy)phenyl]-4-(3-pyridinyl)-2- 0.8pyrimidinamine .sub.--N--[4-[2-(Dimethylamino)ethoxy]phenyl]-4- 0.5(3-pyridinyl)-2-pyrimidinamine .sub.--N--Phenyl-4-(3-pyridinyl)-2-pyrimidinamine, 2.7phosphate .sub.--N'--[4-(2-Furanyl)-2-pyrimidinyl]- .sub.--N, .sub.--N-- 1.9dimethyl-1,4-benzenediamine .sub.--N, .sub.--N--Dimethyl- .sub.--N'--[4-(2-thienyl)-2-pyrimidinyl]- 0.61,4-benzendiamine .sub.--N'--[4-(2,5-Dimethyl-3-furanyl)-2-pyrimidinyl]- 4.9 .sub.--N, .sub.--N--dimethyl-1,4-benzenediamine .sub.--N, .sub.--N--Dimethyl- .sub.--N'--[4-(3-methyl-2-thienyl)-2- 1.8pyrimidinyl]-1,4-benzenediamine .sub.--N--(3,5-Dimethylphenyl)-4-(2-pyridinyl)-2- 0.3pyrimidinamine, phosphate .sub.--N, .sub.--N--Dimethyl- .sub.--N'--[4-(3-pyridinyl)-2-pyrimidin- 1.5yl]-1,4-benzenediamine, trihydrochloride .sub.--N, .sub.--N--Dimethyl- .sub.--N'--[4-(4-pyridinyl)-2-pyrimidin- 3.5yl]-1,3-benzenediamine .sub.--N, .sub.--N--Dimethyl- .sub.--N'--[4-methyl-6-(4-pyridinyl)-2- 37.7pyrimidinyl]-1,4-benzenediamine .sub.--N--[4-[3-Dimethylamino)propoxy]phenyl]-4-(3- 0.5pyridinyl)-2-pyrimidinamine .sub.--N--[4-[2-Diethylamino)ethoxy]phenyl]-4-(3- 0.2pyridinyl)-2-pyrimidinamine .sub.--N--[4-[2-Dimethylamino)ethoxy]phenyl]-4-(3- 0.5pyridinyl)-2-pyrimidinamine, hydrochloride4-[[4-(3-Pyridinyl)-2-pyrimidinyl]amino]ben- 7.6zoic acid .sub.--N, .sub.--N--Dimethyl- .sub.--N'--[4-(2-pyridinyl)-2-pyrimidin- 0.5yl]-1,3-benzenediamine, dihydrochloride .sub.--N, .sub.--N--Dimethyl- .sub.--N'--[4-(2-pyridinyl)-2-pyrimidin- 1.0yl]-1,3-benzenediamine, trihydrochloride .sub.--N--(3,5-Dimethylphenyl)-4-(2-furanyl)-5-methyl- <242-pyrimidinamine .sub.--N, .sub.--N--Dimethyl- .sub.--N'--[4-(4-pyridinyl)-2-pyrimidin- 0.5yl]-1,3-benzenediamine, dihydrochloride .sub.--N'--[4-(2-Furanyl)-5-methyl-2-pyrimidinyl]- .sub.--N, .sub.--N-- 6.1dimethyl-1,4-benzenediamine4-(2-Furanyl)-5-methyl- .sub.--N--phenyl-2-pyrimidin- 5.0amine, sulfate .sub.--N'--[4-(2-Benzofuranyl)-2-pyrimidinyl]- .sub.--N, .sub.--N--di- 5.6methyl-1,4-benzenediamine4-Methyl- .sub.--N--phenyl-6-(2-pyridinyl)-2-pyrimi- 26.8dinamine4-[[4-(4-(Pyridinyl)-2-pyrimidinyl]amino]- 3.3phenol .sub.--N--[4-[2-(Dimethylamino)ethoxy]phenyl]-4-(4- 1.5pyridinyl)-2-pyrimidinamine .sub.--N--[4-[2-(Dimethylamino)ethoxy]phenyl] .sub.--N', .sub.--N'-- 9.1dimethyl- .sub.--N--[4-(4-pyridinyl)-2-pyrimidiny]-1,2-ethanediamine .sub.--N--[4-[3-Dimethylamino)propoxy]phenyl]-4-(4- 1.3pyridinyl)-2-pyrimidinamine .sub.--N--[4-[2-(Diethylamino)ethoxy]phenyl]-4-(4- 0.2pyridinyl)-2-pyrimidinamine4-[2-[(4-Methoxyphenyl)amino]-4-pyrimidinyl]- 33.31-methylpyridinium, iodide .sub.--N, .sub.--N--Dimethyl- .sub.--N'--[4-(4-pyridinyl)-2-pyrimi- 1.0dinyl)]-1,3-benzenediamine, sulfate .sub.--N, .sub.--N--Dimethyl- .sub.--N'--[4-(2-thienyl)-2-pyrimidinyl]- 2.41,3-benzenediamine .sub.--N, .sub.--N--Dimethyl- .sub.--N'--[4-(5-methyl-2-furanyl)-2- 1.6pyrimidinyl]-1,3-benzenediamine .sub.--N'--[4-(2,5-Dimethyl-3-furanyl)-2-pyrimidinyl]- <24 .sub.--N, .sub.--N--dimethyl-1,3-benzenediamine .sub.--N--[2-(Diethylamino)ethyl]-4-[[4-(3-pyridin- 0.8yl)-2-pyrimidinyl]amino]benzamide4-[[4-(4-Pyridinyl)-2-pyrimidinyl]amino]- 5.8phenoxy]acetic acid, ethyl ester .sub.--N, .sub.--N--Diethyl- .sub.--N'--[4-(4-pyridinyl)-2-pyrimidin- 1.1yl]-1,4-benzenediamine .sub.--N, .sub.--N--Dimethyl- .sub.--N'--[4-methyl-6-(2-pyridinyl)-2- 31.8pyrimidinyl]-1,4-benzenediamine .sub.--N--[4-(1 .sub.--H--Imidazol-1-yl)phenyl]-4-(4-pyridin- 12.3yl)-2-pyrimidinamine .sub.--N--[4-(4-Pyridinyl)-2-pyrimidinyl]-1,4-benzene- 3.0diamine, hydrochloride .sub.--N, .sub.--N--Diethyl- .sub.--N'--[4-(3-pyridinyl)-2-pyrimi- 1.7dinyl]-1,4-benzenediamine .sub.--N--[4-(1 .sub.--H--Imidazol-1-yl)phenyl]-4-(3-pyridin- 1.3yl)-2-pyrimidinamine1-[4-[[4-(3-Pyridinyl)-2-pyrimidinyl]amino]- 11.4phenyl]ethanone, oxime1-[4-[[4-(3-Pyridinyl)-2-pyrimidinyl]amino]- 5.1phenyl]ethanone, O--methyloxime .sub.--N, .sub.--N--Diethyl- .sub.--N'--[4-(2-pyridinyl)-2-pyrimidin- 10.1yl]-1,4-benzenediamine .sub.--N--[4-(1 .sub.--H--Imidazol-1-yl)phenyl]-4-(2-pyridin- 1.8yl)-2-pyrimidinamine4-(2-Furanyl)- .sub.--N--[4-(1 .sub.--H--imidazol-1-yl)phenyl]- 2.22-pyrimidinamine .sub.-- N--Methyl-4-[[4-(3-pyridinyl)-2-pyrimidinyl]- 4.6amino]-benzamide .sub.--N, .sub.--N--Dimethyl- .sub.--N'--[4-(5-methyl-2-thienyl)-2- 5.7pyrimidinyl]-1,3-benzenediamine .sub.--N, .sub.--N--Dimethyl- .sub.--N'--[4-(3-thienyl)-2-pyrimidinyl]- 2.11,4-benzenediamine .sub.--N--[1-[4-[[4-(3-Pyridinyl)-2-pyrimidinyl]- 0.4amino]phenyl]ethyl]formamide .sub.--N--[4-[1-Aminoethyl)phenyl]-4-(3-pyridinyl)- 0.82-pyrimidinamine, trihydrochloride4-[[4-(3-Pyridinyl)-2-pyrimidinyl]amino]benz- 0.2enesulfonamide .sub.--N--(3-Chlorophenyl)-4-(4-pyridinyl)-2-pyrimi- 3.1dinamine .sub.--N--(3-Chlorophenyl)-4-(3-pyridinyl)-2-pyrimi- 1.5dinamine .sub.--N--(3-Methoxyphenyl)-4-(3-thienyl)-2-pyrimidin- 1.7amine .sub.--N--Methyl- .sub.--N--[4-[[4-(3-pyridinyl)-2-pyrimidin- 1.1yl]amino]phenyl]acetamide .sub.--N--Methyl- .sub.--N--[4-[[4-(4-pyridinyl)-2-pyrimidin- 0.1yl]amino]phenyl]acetamide .sub.--N--Methyl- .sub.--N--[4-[[4-(2-pyridinyl)-2-pyrimidin- 0.6yl]amino]phenyl]acetamide[4-(2-Furanyl)- .sub.--N--(3-methoxyphenyl)-2-pyrimi- 0.3dinamine4-(2-Benzofuranyl)- .sub.--N--(3-methoxyphenyl)-2- 1.2pyrimidinamineOxo[phenyl[4-(4-pyridinyl)-2-pyrimidinyl]- 2.1amino]acetic acid, ethyl ester .sub.--N--[4-[[4-(4-Pyridinyl)-2-pyrimidinyl]amino]- 5.3phenyl]acetamide .sub.--N, .sub.--N--Dimethyl- .sub.--N'--[4-(2-furanyl)-5-methyl-2- 40pyrimidinyl]-1,3-benzenediamine .sub.--N--[4-[[4-(3-Pyridinyl)-2-pyrimidinyl]amino]- 3.6phenyl]acetamide4-[[4-(2-Pyridinyl)-2-pyrimidinyl]amino]- 4.5benzenesulfonamide .sub.--N--[4-[[4-(2-Pyridinyl)-2-pyrimidinyl]amino]- 1.5phenyl]acetamide .sub.--N--(3-Methoxyphenyl)-4-(2-thienyl)-2-pyrimi- 0.9dinamine .sub.--N--[4-(4-Methyl-1-piperazinyl)phenyl]-4-(3- 1.5pyridinyl)-2-pyrimidinamine .sub.--N--(3-Methoxyphenyl)-4-(5-methyl-2-thienyl)- 2.32-pyrimidinamine .sub.--N--(3-Chlorophenyl)-4-(2-pyridinyl)-2-pyrimi- 1.3dinamine4-(2-Furanyl)- .sub.--N--[4-(4-methyl-1-piperazinyl)- 1.8phenyl]-2-pyrimidinamine .sub.--N--[4-(4-Methyl-1-piperazinyl)phenyl]-4-(4- 0.6pyridinyl)-2-pyrimidinamine .sub.--N--(3-Methoxyphenyl)-4-(2,5-dimethyl-3-furan- 5.8yl)-2-pyrimidinamine .sub.--N--[4-(2-Pyridinyl)-2-pyrimidinyl]-1,4-benzene- 1.0diamine, dihydrochloride .sub.--N--(3-Fluorophenyl)-4-(4-pyridinyl)-2-pyrimi- 0.7dinamine .sub.--N--(3-Fluorophenyl)-4-(3-pyridinyl)-2-pyrimi- 3.3dinamine .sub.--N--(3-Fluorophenyl)-4-(2-pyridinyl)-2-pyrimi- 0.9dinamine1-[3-[[4-(3-Pyridinyl)-2-pyrimidinyl]amino]- 4.1phenyl]ethanone .sub.--N--Methyl- .sub.--N'--[4-(3-pyridinyl)-2-pyrimidinyl]- 2.11,4-benzenediamine .sub.--N--[4-(1-Methylethyl)phenyl]-4-(3-pyridinyl)- 1.12-pyrimidinamine .sub.--N--Methyl- .sub.--N'--[4-(2-pyridinyl)-2-pyrimidinyl]- 1.41,4-benzenediamine .sub.--N--(3-Ethylphenyl)-4-(3-pyridinyl)-2-pyrimi- 1.7dinamine .sub.--N--(3-Ethylphenyl)-4-(2-pyridinyl)-2-pyrimi- 1.4dinamine3-[[4-(2-Pyridinyl)-2-pyrimidinyl]amino]ben- 0.7zenesulfonamide3-[[4-(3-Pyridinyl)-2-pyrimidinyl]amino]ben- 0.2zenesulfonamide .sub.--N--[4-(1,1-Dimethylethyl)phenyl]-4-(2-thienyl)- 4.62-pyrimidinamine .sub.--N, .sub.--N--Diethyl- .sub.--N'--[4-(2-furanyl)-2-pyrimidinyl]- 3.41,4-benzenediamine3-[[4-(4-Pyridinyl)-2-pyrimidinyl]amino]- 0.5benzenesulfonamide .sub.--N, .sub.--N--Dimethyl- .sub.--N'--[4-(4-pyridinyl)-2-pyrimi- 36.2dinyl]-1,2-benzenediamine, fumarate2-[1-[4-[[4-(3-Pyridinyl)-2-pyrimidinyl]amino] 8.1phenyl]ethylidene]hydrazinecarboxamide .sub.--N--[4-[2-[bis(1,1-Dimethylethyl)amino]ethoxy]- 4.6phenyl]-4-(3-pyridinyl)-2-pyrimidinamine -.alpha.-Methyl-4-[[4-(3-pyridinyl)-2-pyrimidinyl]- 4.5amino]benzenemethanol .sub.--N--[1-[3-[[4-(3-Pyridinyl)-2-pyrimidinyl]- 4.6amino]phenyl]ethyl]formamide .sub.--N--[3-[[4-(4-Pyridinyl)-2-pyrimidinyl]amino]- 2.1phenyl]acetamide .sub.--N--[3-[[4-(3-Pyridinyl)-2-pyrimidinyl]amino]- 5.0phenyl]acetamide .sub.--N--[4-(3-Pyridinyl)-2-pyrimidinyl]-1,3-benzene- 0.4diamine, dihydrochloride .sub.--N, .sub.--N--Diethyl- .sub.--N'--[4-(5-methyl-2-furanyl)-2- 28.0pyrimidinyl]1,4-benzenediamine .sub.--N--(3-Methoxyphenyl)-4-(5-methyl-2-furanyl)-2- 1.2pyrimidinamine .sub.--N--[3-[[4-(2-Pyridinyl)-2-pyrimidinyl]amino]- 0.3phenyl]acetamide .sub.--N--[3-(1 .sub.--H--Imidazol-1-yl)phenyl]-4-(2-pyri- 0.1dinyl)-2-pyrimidinamine .sub.--N--[4-(4-Pyridinyl)-2-pyrimidinyl]-1,3-benzene- 1.0diamine .sub.--N--[2-Methyl-4-[[4-(4-pyridinyl)-2-pyrimi- 1.2dinyl]amino]phenyl]acetamide2-Methyl- .sub.--N--[4-(4-pyridinyl)-2-pyrimidinyl]- 0.91,4-benzenediamine, dihydrochloride .sub.--N--[4-(2-Pyridinyl)-2-pyrimidinyl]-1,3-benzene- 0.2diamine .sub.--N--[4-[[4-(5-Methyl-2-thienyl)-2-pyrimidinyl]- 0.3amino]phenyl]acetamide .sub.--N--[3-(1-Aminoethyl)phenyl]-4-(3-pyridinyl)-2- 5.1pyrimidinamine, trihydrochloride .sub.--N--[3-[2-(Diethylamino)ethoxy]phenyl]-4-(3- 2.8pyridinyl)-2-pyrimidinamine .sub.--N--(2-Methoxyphenyl)-4-(3-pyridinyl)-2-pyrimi- 9.8dinamine .sub.--N--[4-[[4-(2-Thienyl)-2-pyrimidinyl]amino]- 0.2phenyl]acetamide .sub.--N--[2-Methyl-4-[4-(3-pyridinyl)-2-pyrimidinyl]- 1.8phenyl]acetamide .sub.--N'--[4-(2-Benzofuranyl)-2-pyrimidinyl]- .sub.--N, .sub.--N-- 6.2diethyl-1,4-benzenediamine .sub.--N--[4-[[4-(2-Furanyl)-2-pyrimidinyl]amino]- 0.7phenyl]acetamide .sub.--N--[4-(1 .sub.--H--Imidazol-1-yl)-3-(trifluoromethyl)- 0.4phenyl]-4-(4-pyridinyl)-2-pyrimidinamine .sub.--N--[3-(1 .sub.--H--Imidazol-1-yl)phenyl]-4-(3-pyri- 0.1dinyl)-2-pyrimidinamine2-[[4-(3-Pyridinyl)-2-pyrimidinyl]amino]- 23.5phenol4-(2-Furanyl)- .sub.--N--[3-(1 .sub.--H--imidazol-1-yl)phenyl]- 0.82-pyrimidinamine .sub.--N--[3-[2-(Diethylamino)ethoxy]phenyl]-4-(2- 1.3furanyl)-2-pyrimidinamine .sub.--N--[4-(1 .sub.--H--Imidazol-1-yl)-3-(trifluoromethyl)- 1.6phenyl]-4-(2-pyridinyl)-2-pyrimidinamine .sub.--N--[3-[2-(Diethylamino)ethoxy]phenyl]-4-(2- 0.6thienyl)-2-pyrimidinamine .sub.--N--[3-[2-(Diethylamino)ethoxy]phenyl]-4-(4- 0.7pyridinyl)-2-pyrimidinamine .sub.--N--[4-(4-Pyridinyl)-2-pyrimidinyl]-1,4-ben- 2.4zenediamineN--[3-(1H--Imidazol-1-yl)phenyl]-4-(4-pyridinyl)- 0.42-pyrimidinamineN--[3-(1H--Imidazol-1-yl)phenyl]-4-(2-thienyl)-2- 0.2pyrimidinamine______________________________________
The ability of these compounds to inhibit lipoxygenase activity in terms of the suppression of the release and biosynthesis of leukotriene B4(LTB4) and 5-hydroxy-eicosatetraenoic acid (5-HETE) was measured as follows.
In this assay 3.times.10.sup.7 peritoneal neutrophils derived from guinea pigs were incubated at 37.degree. C. in Dulbeccos buffer containing 50 mM tris buffer (pH 7.4). Five minutes before the addition of 100 .mu.M arachidonic acid and 20 .mu.M calcium ionophore (A23187), control vehicle or the test compounds were added to the neutrophils at a concentration of 10 .mu.g/ml.
Three minutes after the addition of arachidonic acid and calcium ionophore the total lipid was partitioned into chloroform after adjusting the pH to 3 with citric acid and the addition of equal parts of methanol and chloroform.
The 5-HETE and LTB4 were resolved by HPLC using a 5 .mu.M 4.times.25 cm octadecyl silica column (IBM Instruments) with 70-80% methanol in water adjusted to pH 3.0 with acetic acid. As the mobile phase was pumped at 1.0 ml/minute, LTB4 and 5-HETE were detected by absorbance at 270 and 236 nm, respectively.
LTB4 and 5-HETE were quantitated by comparison with the control and the results were expressed as a percent of control. The lower the percentage, the more active the compound.
The results of this test on representative compounds of this invention appear in Table II.
TABLE II______________________________________Inhibition of Neutrophil Lipoxygenase fromImmunologically Stimulated Guinea Pig Neutrophiles % InhibitionCompound LTB4 5-HETE______________________________________4-(3-Pyridinyl)- --N--[3-trifluoromethyl)- 58.1phenyl]-2-pyrimidinamine --N--(4-Acetylphenyl)-4-(3-pyridinyl)-2- 37.0pyrimidinamine --N--(4-Fluorophenyl)-4-(2-pyridinyl)-2- 45.0pyrimidinamine --N--(4-Methylphenyl)-4-(4-pyridinyl)-2- 45.0pyrimidinamine --N--(4-Fluorophenyl)-4-(4-pyridinyl)-2- 53.0pyrimidinamine4-(3-Pyridinyl)- --N--[3-trifluoromethyl)- 58.0phenyl]-2-pyrimidinamine --N--Phenyl-4-(4-pyridinyl)-2-pyrimidinamine 58.0 --N--(3-Methylphenyl)-4-(3-pyridinyl)-2- 40.0pyrimidinamine --N--[4-Ethylphenyl)-4-(4-pyridinyl)-2- 33.9 41.0pyrimidinamine --N--(4-Ethylphenyl)-4-(2-pyridinyl)-2- 29.5 41.0pyrimidinamine4-(2-Furanyl)- --N--(3-methylphenyl)-2-pyrimi- 7.4 3.0dinamine --N--[4-(4-Methyl-1-piperazinyl)phenyl]-4- 48.0(2-thienyl)-2-pyrimidinamine --N--(4-Ethylphenyl)-4-(6-methyl-3-pyridin- 53.4 54.0yl)-2-pyrimidinamine --N--(3-Methylphenyl)-4-pyrazinyl-2-pyrimi- 50.0dinamine --N--(3-Ethylphenyl)-4-(4-pyridinyl)-2- 36.4 28.7pyrimidinamine --N--(2,3-Dimethylphenyl)-4-(4-pyridinyl)-2- 58.4pyrimidinamine --N--Phenyl-4-(3-thienyl)-2-pyrimidinamine 56.0 --N--(3-Methylphenyl)-4-(3-thienyl)-2-pyrimi- 48.0dinamine --N--(4-Ethylphenyl)-4-(3-thienyl)-2-pyrimi- 56.0dinamine --N--(2,4-Dimethylphenyl)-4-(2-pyridinyl)-2- 54.0pyrimidinamine --N--(3,5-Dimethylphenyl)-4-(4-pyridinyl)-2- 53.1 54.0pyrimidinamine --N--(2-Methoxyphenyl)-4-(4-pyridinyl)-2- 17.4 21.0pyrimidinamine --N--(2,5-Dimethoxyphenyl)-4-(4-pyridinyl)- 43.2 47.02-pyrimidinamine --N--(2,4-Dimethylphenyl)-4-(4-pyridinyl)-2- 37.0 43.0pyrimidinamine --N--(2-Methoxy-5-methylphenyl)-4-(2-pyridin- 54.0yl)-2-pyrimidinamine4-(4-Pyridinyl)- --N--(2,4,6-trimethylphenyl)- 53.62-pyrimidinamine4-(2-Furanyl)- --N--(4-methoxyphenyl)-2- 44.0pyrimidinamine4-(2-Furanyl)- --N--[3-trifluoromethyl)- 45.0 49.0phenyl]-2-pyrimidinamine --N--(4-Fluorophenyl)-4-(2-furanyl)-2-pyrimi- 33.0dinamine --N--Phenyl-4-(4-pyridinyl)-2-pyrimidinamine, 58.0compound with 2-hydroxy-1,2,3-propanetri-carboxylate (2:1) --N--[(3,4-Dimethylphenyl)methyl]-4-(4- 24.0 36.0pyridinyl)-2-pyrimidinamine --N--Phenyl-4-(4-pyridinyl)-2-pyrimidinamine, 56.0sulfate4-(2-Benzofuranyl)- --N--(3-methylphenyl)-2- 46.1pyrimidinamine --N--(4-Ethylphenyl)-4-(4-pyridinyl)-2- 19.0pyrimidinamine --N--(3,4-Dimethylphenyl)-4-(4-pyridinyl)-2- 19.0pyrimidinamine --N--(3,4-Dimethylphenyl)-4-(2-pyridinyl)-2- 17.3 35.0pyrimidinamine --N--(4-Fluorophenyl)-4-(3-thienyl)-2-pyrimi- 51.6dinamine4-(10 --H--Phenolthiazin-2-yl)- --N--phenyl-2- 48.0pyrimidinamine4-(1 --H--Indol-3-yl)- --N--phenyl-2-pyrimidin- 41.2 39.0amine --N--(2-Methoxy-5-methylphenyl)-4-(4-pyridin- 44.7 37.0yl)-2-pyrimidinamine --N--(3-Methylphenyl)-4-(1-methyl-1 --H--pyrrol- 60.02-yl)-2-pyrimidinamine4-(1-Methyl-1 --H--pyrrol-2-yl)- --N--phenyl-2- 57.0pyrimidinamine --N--(4-Ethylphenyl)-4-(1 --H--indol-3-yl)-2- 56.5pyrimidinamine --N--[1,1'-Biphenyl]-4-yl-(4-pyridinyl)-2- 37.1 45.0pyrimidinamine4-[[4-(4-Pyridinyl)-2-pyrimidinyl]-amino]- 45.2 47.0benzoic acid, methyl ester --N--(3-Methylphenyl)-4-(4-quinolinyl)-2- 16.0pyrimidinamine --N--Phenyl-4-(4-quinolinyl)-2-pyrimidinamine 46.4 57.0 --N--(4-Ethylphenyl)-4-(4-quinolinyl)-2- 58.0pyrimidinamine --N--(3,5-Dimethylphenyl)-4-(2-furanyl)-2- 56.1pyrimidinamine --N--[4-(1,1-Dimethylethyl)phenyl]-4-(4- 47.8 54.0pyridinyl)-2-pyrimidinamine --N--Methyl- --N--phenyl-4-(2-pyridinyl)-2- 58.1 54.0pyrimidinamine --N--Phenyl-4-(1 --H--pyrrol-2-yl)-2-pyrimidin- 55.4amine --N--(4-Ethylphenyl)-4-(1 --H--pyrrol-2-yl)-2- 32.6 54.0pyrimidinamine4-(3-Pyridinyl)- --N--[3-(trifluoromethyl)- 37.3 49.0phenyl]-2-pyrimidinamine sulfate --N--Phenyl-4-(4-pyridinyl)-2-pyrimidinamine, 48.0 43.0dihydrochloride4-(3-Methyl-2-thienyl)- --N--phenyl-2-pyrimi- 59.0dinamine4-(5-Methyl-2-furanyl)- --N--(3-methylphenyl)- 59.62-pyrimidinamine4-Methyl-6-(5-methyl-2-thienyl)- --N--phenyl- 42.3 52.02-pyrimidinamine --N--[4-(Dimethylamino)phenyl]-4-(4-pyridin- 16.6 12.4yl)-2-pyrimidinamine --N--(3-Methoxyphenyl)-4-(4-pyridinyl)-2- 31.2 50.0pyrimidinamine --N--[4-(Dimethylamino)phenyl]-4-(2-pyridin- 20.1 17.2yl)-2-pyrimidinamine --N--(3,5-Dimethylphenyl)-4-(3-pyridinyl)- 50.7 56.02-pyrimidinamine4-[[4-(3-Pyridinyl)-2-pyrimidinyl]amino]- 35.8 47.0benzoic acid, ethyl ester --N, --N--Dimethyl- --N--'[4-(3-pyridinyl)-2-pyrimi- 43.4 34.0dinyl]-1,4-benzenediamine4-(2,5-Dimethyl-3-furanyl)- --N--phenyl-2- 46.9 56.0pyrimidinamine --N, --N--Dimethyl- --N'--[4-(4-pyridinyl)-2- 40.7 37.0pyrimidinyl]-1,4-benzenediamine, trihydro-chloride --N, --N--Dimethyl- --N'--[4-(2-pyridinyl)-2-pyrimi- 37.6 39.0dinyl]-1,4-benzenediamine, dihydrochloride4-[[4-(3-Pyridinyl)-2-pyrimidinyl]amino]- 30.0phenol3-[[4-(3-Pyridinyl)-2-pyrimidinyl]amino]- 36.1 50.0benzoic acid, ethyl ester --N--(3,5-Dimethylphenyl)-4-(2-pyridinyl)-2- 50.0pyrimidinamine, sulfate --N--[4-(2-Propenyloxy)phenyl]-4-(3-pyridin- 34.1yl)-2-pyrimidinamine --N'[4-(2-Furanyl)-2-pyrimidinyl]- --N, --N--dim- 16.9 16.9ethyl-1,4-benzenediamine --N, --N--Dimethyl- --N'--[4-(2-thienyl)-2-pyrimi- 49.8 17.8dinyl]-1,4-benzenediamine --N'--[4-(2,5-Dimethyl-3-furanyl)-2-pyrimi- 21.6 17.0dinyl]- --N, --N--dimethyl-1,4-benzenediamine --N, --N--Dimethyl- --N'--[4-(3-methyl-2-thienyl)- 16.4 13.62-pyrimidinyl]-1,4-benzenediamine --N, --N--Dimethyl --N'--[4-(3-pyridinyl)-2-pyrimi- 46.8 42.0dinyl]-1,4-benzenediamine, trihydro-chloride --N, --N--Dimethyl- --N'--[4-(4-pyridinyl)-2-pyrimi- 51.1dinyl]-1,3-benzenediamine --N, --N--Dimethyl-N'--[4-methyl-6-(4-pyridin- 1.6 10.0yl)-2-pyrimidinyl]-1,4-benzenediamine --N--(3,5-Dimethylphenyl)-4-methyl-6-(3- 32.7 40.0pyridinyl)-2-pyrimidinamine --N'--[4-(2-Furanyl)-5-methyl-2-pyrimidinyl]- 3.6 --N, --N--dimethyl-1,4-benzendiamine4-(2-Furanyl)-5-methyl- --N--phenyl-2-pyrimi- 52.4dinamine, sulfate --N'--[4-(2-Benzofuranyl)-2-pyrimidinyl]- --N, --N-- 22.9 30.0dimethyl-1,4-benzenediamine4-Methyl- --N--phenyl-6-(2-pyridinyl)-2- 30.3 42.0pyrimidinamine4-[[4-(4-Pyridinyl)-2-pyrimidinyl]-amino]- 36.0phenol --N--(4-Methoxyphenyl)- --N--methyl-4-(4- 57.4pyridinyl-2-pyrimidinamine --N, --N--Dimethyl- --N'--[4-(2-thienyl)-2-pyrimi- 39.6 50.0dinyl]-1,3-benzenediamine --N, --N--Dimethyl- --N'--[4-(5-methyl-2-furanyl)-2- 31.1 37.7pyrimidinyl]-1,3-benzenediamine --N--Methyl- --N'--[4-(2-pyridinyl)-2-pyrimi- 24.1 53.6dinyl]-1,4-benzenediamine --N--[1-[3-[[4-(3-Pyridinyl)-2-pyrimidinyl]- 34.0amino]phenyl]ethyl]formamide --N--[4-[[4-(5-Methyl-2-thienyl)-2-pyrimi- 51.0 46.0dinyl]amino]phenyl]acetamide --N'--[4-(2-Benzofuranyl)-2-pyrimidinyl]- --N, --N-- 51.0 45.0diethyl-1,4-benzenediamine --N--[4-(1 --H--Imidazol-1-yl)-3-(trifluoro- 20.0 16.0methyl)phenyl]-4-(4-pyridinyl)-2-pyrimidinamine --N--[4-(5-Methyl-2-thienyl)-2-pyrimidinyl]- 47.0 28.01,4-benzenediamine, dihydrochloride --N--[3-(1 --H--Imidazol-1-yl)phenyl]-4-(3-pyri- 50.0 51.0dinyl)-2-pyrimidinamine --N--[3-(1 --H--Imidazolyl)phenyl]-4-(2-thienyl)- 50.0 39.02-pyrimidinamine --N--[4-(2-Furanyl)-2-pyrimidinyl]-1,4-ben- 54.0zenediamine, dihydrochloride --N--[4-(1 --H--Imidazol-1-yl)-3-(trifluoro- 19.0methyl)phenyl]-4-(2-pyridinyl)-2-pyrimi-dinamine4-[ [4-(2-Furanyl)-2-pyrimidinyl]amino]- 47.0benzenesulfonamide______________________________________
The novel compounds of the present invention are effective as antiasthmatic agents in mammals when administered in amounts ranging from about 0.1 mg to about 100 mg/kg of body weight per day. A preferred dosage regimen for optimum results would be from about 0.1 mg to about 25 mg/kg of body weight per day, and such dosage units are employed that a total of from about 7 mg to about 1.8 g of the active compound for a subject of about 70 kg of body weight are administered in a 24 hour period. This dosage regimen may be adjusted to provide the optimum therapeutic response. For example, several divided doses may be administered daily or the dose may be proportionally reduced as indicated by the exigencies of the therapeutic situation. A decided practical advantage is that these active compounds may be adminstered in any convenient manner such as by the oral, aerosol, intravenous, intramuscular, or subcutaneous routes.
The active compounds may be orally administered, for example, with an inert diluent or with an assimilable edible carrier, or they may be enclosed in hard or soft shell gelatin capsules, or they may be compressed into tablets or they may be incorporated directly with the food of the diet. For oral therapeutic administration, these active compounds may be incorporated with excipients and used in the form of ingestible tablets, buccal tablets, troches, capsules, elixirs, suspensions, syrups, wafers and the like. Such compositions and preparations should contain at least 0.1% of active compound. The percentage of the compositions and preparations may, of course, be varied and may conveniently be between about 2% to about 60% of the weight of the unit. The amount of active compound is such therapeutically useful compositions is such that a suitable dosage will be obtained. Preferred compositions or preparations according to the present invention are prepared so that an oral dosage unit form contains between about 5 and 200 mg of active compound.
The tablets, troches, pills, capsules and the like may also contain the following: A binder such as gum tragacanth, acacia, corn starch or gelatin; excipients such as dicalcium phosphate; a disintegrating agent such as corn starch, potato starch, alginic acid and the like; a lubricant such as magnesium stearate; and a sweetening agent such as sucrose, lactose or saccharin may be added or a flavoring agent such as peppermint, oil of wintergreen or cherry flavoring. When the dosage unit form is a capsule, it may contain, in addition to materials of the above type, a liquid carrier. Various other materials may be present as coatings or to otherwise modify the physical form of the dosage unit. For instance, tablets, pills or capsules may be coated with shellac, sugar or both. A syrup or elixir may contain the active compound, sucrose as a sweetening agent, methyl and propylparabens as preservatives, a dye and flavoring such as cherry or orange flavor. Of course, any material used in preparing any dosage unit form should be pharmaceutically pure and substantially non-toxic in the amounts used. In addition, these active compounds may be incorporated into sustained-release preparations and formulations.
Compositions according to the present invention having the desired clarity, stability and adaptability for parenteral use are obtained by dissolving from 0.10% to 10.0% by weight of active compound in a vehicle consisting of a polyhydric aliphatic alcohol or mixtures thereof. Especially satisfactory are glycerin, propylene glycol, and polyethylene glycols. The polyethylene glycols consist of a mixture of non-volatile, normally liquid, polyethylene glycols which are soluble in both water and organic liquids and which have molecular weights of from about 200 to 1500. Although various mixtures of the aforementioned non-volatile polyethylene glycols may be employed, it is preferred to use a mixture having an average molecular weight of from about 200 to about 400.
In addition to the active compound, the parenteral solutions may also contain various preservatives which may be used to prevent bacterial and fungal contamination. The preservatives which may be used for these purposes are, for example, myristyl-gamma-picolinium chloride, benzalkonium chloride, phenethyl alcohol, p-chlorophenyl-alpha-glycerol ether, methyl and propyl parabens, and thimerosal. As a practical matter, it is also convenient to employ antioxidants. Suitable antioxidants include, for example, sodium bisulfite, sodium metabisulfite, and sodium formaldehyde sulfoxylate. Generally, from about 0.05% to about 0.2% concentrations of anti-oxidant are employed.
These compounds may also be administered by inhalation using conventional Aerosol.RTM. formulations.

The invention will be described in greater detail in conjunction with the following specific examples.
EXAMPLE 1
4-(3-Pyridinyl)-N-[3-(trifluoromethyl)phenyl]-2-pyrimidinamine
A 7.04 g amount of 3-dimethylamino-1-(3-pyridinyl)-2-propen-1-one (U.S. Pat. No. 4,281,000) and 18.72 g of [3-(trifluoromethyl)phenyl]guanidine carbonate in 500 ml of n-propanol was heated at reflux temperature for 16 hours. The solvent was evaporated to near dryness, then water was added and the precipitate which formed was collected by filtration, then recrystallized from hexane to give 5.55 g of the desired product, mp 170.degree.-171.degree. C.
EXAMPLE 2
N-(4-Methoxyphenyl)-4-(3-pyridinyl)-2-pyrimidinamine
A mixture of 14.4 g of 3-dimethylamino-1-(3-pyridinyl)-2-propen-1-one and 16.1 g of 4-methoxyphenyl guanidine carbonate in 200 ml of isopropanol was heated at reflux for 20 hours. The reaction mixture was cooled, the crude product was collected by filtration and washed with water. The material was recrystallized from isopropanol to give the desired product as light yellow crystals, mp 121.degree.-122.degree. C.
EXAMPLE 3
N-(4-Methoxyphenyl)-4-(4-pyridinyl)-2-pyrimidinamine
A 14.4 g amount of 3-dimethylamino-1-(4-pyridinyl)-2-propen-2-one (U.S. Pat. No. 4,281,000) and 16.1 g of 4-methoxyphenylguanidine carbonate in 200 ml of isopropanol was heated at reflux for 24 hours. The solvent was evaporated to 1/3 volume, then the mixture was cooled in an ice-bath to crystallize the product. The product was collected by filtration and washed with water, then with isopropanol. The material was recrystallized from isopropanol/ethylene glycol monomethyl ether to give 16.7 g of the desired product as yellow crystals, mp 174.degree.-175.degree. C.
EXAMPLE 4
N-(4-Methoxyphenyl)-4-(2-thienyl)-2-pyrimidinamine
A mixture of 10.9 g of 3-dimethylamino-1-(2-thienyl)-2-propen-1-one (U.S. Pat. No. 4,374,988) and 11.8 g of 4-methoxyphenylguanidine carbonate in 150 ml of isopropanol was heated at reflux for 48 hours. The solution was cooled, then filtered, giving 9.0 g of the desired product as yellow crystals, mp 158.degree.-160.degree. C.
EXAMPLE 5
4-[[4-(4-Pyridinyl)-2-pyrimidinyl]amino]benzoic acid, methyl ester
A solution of 10.0 g of 4-guanidinobenzoic acid, hydrochloride in 310 ml of methanol was mixed with 6.0 ml (9.68 g) of thionyl chloride at 0.degree. C. for 15 minutes, then stirred for one hour at room temperature and then heated at reflux for 16 hours. The solvent ws removed in vacuo and the solid was washed with ether and air dried to give 11.4 g of white crystals (A).
The above procedure was repeated using 20.0 g of 4-guanidinobenzoic acid, 11.9 ml (19.4 g) of thionyl chloride and 600 ml of methanol to give 22.6 g of white crystals (B).
The products (A) and (B) were combined and recrystallized from absolute ethanol. The product was washed with cold absolute ethanol and air dried giving 26.2 g of p-guanidinobenzoic acid, methyl ester, hydrochloride as white crystals, mp 137.degree.-138.5.degree. C. (dec.).
A 9.15 g amount of the above compound was partially dissolved in 100 ml of methanol (stored over 4A molecular sieves) and 2.15 g of sodium methoxide was added. The mixture was stirred briefly, then 7.0 g of 3-dimethylamino-1-(4-pyridinyl)-2-propen-1-one was added and the mixture was heated under argon with stirring for 21.5 hours. The reaction mixture was cooled in an ice bath, then filtered and washed with cold methanol. The residue was dissolved in a mixture of dichloromethane and methanol and filtered to remove sodium chloride. The filtrate was concentrated on a steam bath until crystal formation. The mixture was allowed to stand at room temperature for 16 hours then was filtered. The precipitate was washed with ice cold methanol then dried and gave 5.8 g of the desired product, mp 194.5.degree.-196.5.degree. C.
EXAMPLE 6
3-Dimethylamino-1-(3-indolyl)-2-propen-1-one
A mixture of 3.18 g of 3-acetylindole and 5.17 ml. (4.36 g) of tert-butoxybis(dimethylamino)methane was heated on a steam bath for 4 hours. The cooled reaction mixture was triturated with n-hexanes and gave a semi-solid. The solvent was removed in vacuo and the material was triturated with dichloromethane giving 3.08 g of the desired compound as a tan crystalline solid, mp 239.degree.-245.degree. C.
EXAMPLE 7
3-Dimethylamino-1-(5-methyl-2-thienyl)-2-propen-1-one
A mixture of 56.08 g of 2-acetyl-5-methylthiophene and 250 ml of N,N-dimethylformamide dimethylacetal was heated on a steam bath under an air condenser for 16 hours. The mixture was cooled in an ice bath and filtered giving 66.82 g of the desired compound, mp 118.degree.-121.degree. C.
EXAMPLE 8
3-(Dimethylamino)-1-(5-methyl-2-furanyl)-2-propen-1-one
A mixture of 37.24 g of 2-acetyl-5-methylfuran and 150 ml of N,N-dimethylformamide dimethylacetal was heated on a steam bath under an air condenser for 16.5 hours. The solvent was removed in vacuo and the residue taken up in dichloromethane and passed through a short column of magnesium silicate. The filtrate was evaporated on a steam bath with the addition of n-hexanes to a volume of 100-150 ml. Cooling with scratching gave 28.31 g of the desired compound, mp 123.degree.-125.degree. C.
EXAMPLE 9
3-(Dimethylamino)-1-(1H-pyrrol-2-yl)-(E)-2-propen-1-one
A mixture of 39.6 g of 2-acetylpyrrole and 104 ml (87.7 g) of tert-butoxy bis(dimethylamino)methane was heated on a steam bath for 20 minutes. The reaction was allowed to subside, then heating was continued for 6 hours. The mixture solidified then was slurried in hexane with chilling. The crude product was collected, washed with hexane and dried. The solid was dissolved in chloroform containing 5% methanol and filtered through magnesium silicate. The eluent was evaporated in vacuo and the residue was recrystallized from dichloromethane/hexane containing a small amount of methanol. The solid was collected, washed with hexane then dried in vacuo giving 25.1 g of the desired compound as yellow crystals, mp 192.degree.-193.degree. C. (dec.).
The following 3-(dimethylamino)acrylophenone intermediate compounds listed in Table III were prepared in a similar manner to the procedures described in Examples 6-8 and by those described in U.S. Pat. Nos. 4,281,000, 4,374,988 and in Ser. No. 672,753, filed on Nov. 19, 1984.
TABLE III______________________________________3-(Dimethylamino)acrylophenone Intermediates ##STR5## ##STR6##Ex. R.sub.3 R.sub.4 R.sub.5 MP .degree.C.______________________________________10 2-Furanyl H H 84-8611 2-pyridinyl H H 127-13012 2-furanyl CH.sub.3 H Oil13 4-pyridinyl CH.sub.3 H 106-10814 4-methyl-3-pyridinyl H H 116-11815 4-methyl-3-pyridinyl H CH.sub.3 119-12016 2-pyrazinyl H H 132-13317 3-thienyl H H 89-9018 4-quinolinyl H H19 3-methyl-2-thienyl H H 45-4920 1-methyl-1 --Hpyrrol-2-yl H H 94-9521 5-methyl-2-thienyl H CH.sub.3 123-12622 2,5-dimethyl-3-furanyl H H 91-9523 2-pyridinyl H CH.sub.3 68-7024 2-thienyl H CH.sub.3 97-9925 4-pyridinyl H CH.sub.3 88-8926 3-pyridinyl H CH.sub.3 62-6427 3-pyridinyl CH.sub.3 H 76-7828 3-methyl-2-pyridinyl H H 97-9829 2-benzofuranyl H H 137.0-138.530 3-pyridinyl H H 97-9931 2-phenothiazine H H______________________________________
EXAMPLES 32-251
4,5,6-Substituted-2-pyrimidinamines
The following 4,5,6-substituted-2-pyridinamine final products listed in Table IV were obtained by reacting a 3-(dimethylamino)acrylophenone from Table III and an appropriately substituted phenylguanidine base, carbonate, sulfate, nitrate or hydrochloride salt in an inert solvent such as absolute ethanol, n-propanol, isopropanol, 2-methoxyethanol, or n-butanol and the like, with or without a base such as sodium hydroxide, potassium hydroxide or potassium carbonate and the like by heating at the reflux temperature for from 6-90 hours, then recovering the product in a conventional manner with recrystallization from solvents such as n-propanol, isopropanol, absolute ethanol and the like.
TABLE IV__________________________________________________________________________2-Amino-4,5,6-substituted Pyrimidinamines Acrylophenone PhenylguanidineEx. Source Precursor Product MP.degree. C.__________________________________________________________________________ 32 Ex. 12 Phenylguanidine carbonate 4-(2-Furanyl)-5-methyl- .sub.-- .sub.--N--phenyl- 2- 141-142 pyrimidinamine 33 Ex. 3 [3-(Trifluoromethyl)- 4-(4-Pyridinyl)- .sub.-- .sub.--N--[3-(trifluoro- 198-200 phenyl]guanidine carbonate methyl)phenyl]-2-pyrimidinamine 34 Ex. 1 Phenylguanidine carbonate .sub.-- .sub.--N--Phenyl-4-(3-pyridinyl)-2-pyrim i- 147-148 dinamine 35 Ex. 1 (4-Acetylphenyl)guanidine .sub.--N--(4-Acetylphenyl)-4-(3-pyridinyl)- 181-183 2-pyrimidinamine 36 Ex. 1 (4-Fluorophenyl)guanidine .sub.--N--(4-Fluorophenyl)-4-(3-pyridinyl)- 167-169 carbonate 2-pyrimidinamine 37 Ex. 11 (4-Methoxyphenyl)guani- .sub.--N--(4-Methoxyphenyl)-4-(2-pyridinyl)- 162-164 dine carbonate 2-pyrimidinamine 38 Ex. 3 (4-Fluorophenyl)guanidine .sub.--N--(4-Fluorophenyl)-4-(4-pyridinyl)- 168-188 carbonate 2-pyrimidinamine 39 Ex. 1 (4-Bromophenyl)guanidine .sub.--N--(4-Bromophenyl)-4-(3-pyridinyl)- 174-175 carbonate 2-pyrimidinamine 40 Ex. 4 (4-Fluorophenyl)guanidine .sub.--N--(4-Fluorophenyl)-4-(2-thienyl)-2- 176-178 carbonate pyrimidinamine 41 Ex. 11 [3-(Trifluoromethyl)- 4-(2-Pyridinyl)- .sub.--N--[3-(trifluoro- 161-162 phenyl]guanidine carbonate methyl)phenyl]-2-pyrimidinamine 42 Ex. 4 Phenylguanidine carbonate .sub.--N--Phenyl-4-(2-thienyl)-2-pyrimidin- 137-139 amine 43 Ex. 1 3-Chloro-4-methylphenyl- .sub.--N--(3-Chloro-4-methylphenyl)-4-(3- 140-145 guanidine carbonate pyridinyl)-2-pyrimidinamine 44 Ex. 11 3-Methylphenylguanidine .sub.--N--(3-Methylphenyl)-4-(2-pyridinyl)- 135-137 carbonate 2-pyrimidinamine 45 Ex. 3 3-Methylphenylguanidine .sub.--N--(3-Methylphenyl)-4-(4-pyridinyl)- 157-159 carbonate 2-pyrimidinamine 46 Ex. 3 Phenylguanidine carbonate .sub.--N--Phenyl-4-(4-pyridinyl)-2-pyrimi- 153-154 dinamine 47 Ex. 1 3-Methylphenylguanidine .sub.--N--(3-Methylphenyl)-4-(3-pyridinyl)- 102-103 carbonate 2-pyrimidinamine 48 Ex. 3 4-Ethylphenylguanidine .sub.--N--(4-Ethylphenyl)-4-(4-pyridinyl)- 138-140 carbonate 2-pyrimidinamine 49 Ex. 13 4-Ethylphenylguanidine .sub.--N--(-4-Ethylphenyl)-5-methyl-4-(4- 132-133 carbonate pyridinyl)-2-pyrimidinamine 50 Ex. 3 3,4-Dichlorophenylguani- .sub.--N--(3,4-Dichlorophenyl)-4-(4-pyri- 214-216 dine carbonate dinyl)-2-pyrimidinamine 51 Ex. 1 4-Ethylphenylguanidine .sub.--N--(4-Ethylphenyl)-4-(3-pyridinyl)- 120-122.5 carbonate 2-pyrimidinamine 52 Ex. 11 4-Ethylphenylguanidine .sub.--N--(4-Ethylphenyl)-4-(2-pyridinyl)- 148.5-149.5 carbonate 2-pyrimidinamine 53 Ex. 4 3-Methylphenylguanidine .sub.--N--(3-Methylphenyl)-4-(2-thienyl)- 112.5-114.5 carbonate 2-pyrimidinamine 54 Ex. 10 Phenylguanidine carbonate 4-(2-Furanyl)- .sub.--N--phenyl-2-pyrimidin- 144-145 amine 55 Ex. 10 3-Methylphenylguanidine 4-(2-Furanyl)- .sub.--N--(3-methylphenyl)- 98-99.5 carbonate 2-pyrimidinamine 56 Ex. 14 4-Ethylphenylguanidine .sub.--N--(4-Ethylphenyl)-4-(6-methyl-3- 154-155 carbonate pyridinyl)-2-pyrimidinamine 57 Ex. 15 4-Ethylphenylguanidine .sub.--N--(4-Ethylphenyl)-6-methyl-4-(6- 118-120 carbonate methyl-3-pyridinyl)-2-pyrimidin- amine 58 Ex. 16 4-Ethylphenylguanidine .sub.--N--(4-Ethylphenyl)-4-pyrazinyl-2- 157.5-159 carbonate pyrimidinamine 59 Ex. 16 3-Methylphenylguanidine .sub.--N--(3-Methylphenyl)-4-(4-pyrazinyl)- 112.5-117 carbonate 2-pyrimidinamine 60 Ex. 3 2-Methylphenylguanidine .sub.--N--(2-Methylphenyl)-4-pyrazinyl)-2- 129-130.5 carbonate pyrimidinamine 61 Ex. 3 3-Ethylphenylguanidine .sub.--N--(3-Ethylphenyl)-4-(4-pyridinyl)- 126-128 sulfate 2-pyrimidinamine 62 Ex. 3 2,5-Dimethylphenylguani- .sub.--N--(2,5-Dimethylphenyl)-4-(4-pyri- 131-134 dine carbonate dinyl-2-pyrimidinamine 63 Ex. 3 2,3-Dimethylphenylguani- .sub.--N--(2,3-Dimethylphenyl)-4-(4-pyri- 121-123 dine carbonate dinyl)-2-pyrimidinamine 64 Ex. 17 3-Methylphenylguanidine .sub.--N--(3-Methylphenyl)-4-(3-thienyl)- 104.5-105.5 carbonate 2-pyrimidinamine 65 Ex. 11 2,5-Dimethylphenylguani- .sub.--N--(2,5-Dimethylphenyl)-4-(2-pyri- 139-142 dine carbonate dinyl)-2-pyrimidinamine 66 Ex. 3 3,5-Dimethylphenylguani- .sub.--N--(3,5-Dimethylphenyl)-4-(4-pyri- 183-185 dine carbonate dinyl)-2-pyrimidinamine 67 Ex. 3 1- .sub.--Naphthylguanidine .sub.--N--1- .sub.--Naphthalenyl-4-(4-pyridinyl) - 174-176 nitrate 2-pyrimidinamine 68 Ex. 11 3,5-Dimethylphenylguani- .sub.--N--(3,5-Dimethylphenyl)-4-(2- 114-119 dine hydrochloride pyridinyl)-2-pyrimidinamine 69 Ex. 11 1- .sub.--Naphthylguanidine .sub.--N--1- .sub.--Naphthalenyl-4-(2-pyridinyl) - 135-138 nitrate 2-pyrimidinamine 70 Ex. 3 2,4-Dimethylphenylguani- .sub.--N--(2,4-Dimethylphenyl)-4-(4-pyri- 116-118 dine carbonate dinyl)-2-pyrimidinamine 71 Ex. 3 2,4,6-Trimethylphenyl- 4-(4-Pyridinyl)- .sub.--N--(2,4,6-trimethyl- 142-144 guanidine carbonate phenyl)-2-pyrimidinamine 72 Ex. 10 4-Methoxyphenylguanidine 4-(2-Furanyl)- .sub.--N--(4-methoxyphenyl)- 155-158.5 carbonate 2-pyrimidinamine 73 Ex. 10 [3-(Trifluoromethyl)- 4-(2-Furanyl)- .sub.--N--[3-(trifluorometh- 150-154 phenyl]guanidine carbonate yl)phenyl]-2-pyrimidinamine 74 Ex. 10 4-Fluorophenylguanidine .sub.--N--(4-Fluorophenyl)-4-(2-furanyl)- 150-152 carbonate 2-pyrimidinamine 75 Ex. 11 .sub.--N--Cyclopentylguanidine .sub.--N--Cyclopentyl-4-(2-pyridinyl)-2- 106-109 sulfate pyrimidinamine 76 Ex. 11 3,4-Dimethylphenylguani- .sub.--N--(3,4-Dimethylphenyl)-4-(2-pyri- 130-133.5 dine carbonate dinyl)-2-pyrimidinamine 77 Ex. 17 4-Methoxyphenylguanidine .sub.--N--(4-Methoxyphenyl)-4-(3-thienyl)- 158-160.5 carbonate 2-pyrimidinamine 78 Ex. 10 3-Ethylphenylguanidine .sub.--N--(3-Ethylphenyl)-4-(2-furanyl)-2- 95-98 sulfate pyrimidinamine 79 Ex. 6 Phenylguanidine carbonate 4-(1H--Indol-3-yl)- .sub.--N--phenyl-2- 188-190 pyrimidinamine 80 Ex. 3 2-Methoxy-5-methylphenyl- .sub.-- N--(2-Methoxy-5-methylphenyl)-4-(4- 96-98.5 guanidine carbonate pyridinyl)-2-pyrimidinamine 81 Ex. 20 3-Methylphenylguanidine .sub.--N--(3-Methylphenyl)-4-(1-methyl-1H-- 117-120 carbonate pyrrol-2-yl)-2-pyrimidinamine 82 Ex. 20 4-Ethylphenylguanidine .sub.--N--(4-Ethylphenyl)-4-(1-methyl-1H-- 89-91 carbonate pyrrol-2-yl)-2-pyrimidinamine 83 Ex. 20 Phenylguanidine carbonate 4-(1-Methyl-1H--pyrrol-2-yl)- .sub.--N-- 118-120 phenyl-2-pyrimidinamine 84 Ex. 4 3-Ethylphenylguanidine .sub.--N--(3-Ethylphenyl)-4-(2-thienyl)-2- 114-116 sulfate pyrimidinamine 85 Ex. 17 3-Ethylphenylguanidine .sub.--N--(3-Ethylphenyl)-4-(3-thienyl)- 86-89 sulfate 2-pyrimidinamine 86 Ex. 6 3-Methylphenylguanidine 4-(1H--Indol-2-yl)- .sub.--N--(3-methylphen- 164-167 carbonate yl)-2-pyrimidinamine 87 Ex. 18 3-Methylphenylguanidine .sub.--N--(3-Methylphenyl)-4-(4-quinolin- 196-198 carbonate yl)-2-pyrimidinamine 88 Ex. 18 Phenylguanidine carbonate .sub.--N--Phenyl-4-(4-quinolinyl)-2-pyrimi- 182-184 dinamine 89 Ex. 18 4-Ethylphenylguanidine .sub.--N--(4-Ethylphenyl)-4-(4-quinolinyl)- 176-178 carbonate 2-pyrimidinamine 90 Ex. 10 3,5-Dimethylphenylguani- .sub.--N--(3,5-Dimethylphenyl)-4-(2-fur- 126-129 dine hydrochloride anyl)-2-pyrimidinamine 91 Ex. 4 3,5-Dimethylphenylguani- .sub.--N--(3,5-Dimethylphenyl)-4-(2-thien- 152-155 dine hydrochloride yl)-2-pyrimidinamine 92 Ex. 3 .sub.--N--Methyl- .sub.--N--phenylguani- .sub.--N--Methyl- .sub.--N--phenyl-4-(4-pyridiny l)- 105-107 dine hydrochloride 2-pyrimidinamine 93 Ex. 3 2,4-Difluorophenylguani- .sub.--N--(2,4-Difluorophenyl)-4-(4-pyri- 172-174 dine hydrochloride dinyl)-2-pyrimidinamine 94 Ex. 1 2,4-Difluorophenylguani- .sub.--N--(2,4-Difluorophenyl)-4-(3-pyri- 163-165 dine hydrochloride dinyl)-2-pyrimidinamine 95 Ex. 7 3-Methylphenylguanidine .sub.--N--(3-Methylphenyl)-4-(5-methyl-2- 114-116 carbonate thienyl)-2-pyrimidinamine 96 Ex. 3 2,6-Difluorophenylguani- .sub.--N--(2,6-Difluorophenyl)-4-(4-pyri- 174-176 dine hydrochloride dinyl)-2-pyrimidinamine 97 Ex. 9 Phenylguanidine carbonate .sub.--N--Phenyl-4-(1H--pyrrol-2-yl)-2- 154-157 pyrimidinamine 98 Ex. 1 4-Tert-butylphenylguani- .sub.--N--.nu.-(1,1-Dimethylethyl)phenyl]-4- 130-133 dine sulfate (3-pyridinyl)-2-pyrimidinamine 99 Ex. 1 2,6-Difluorophenylguani- .sub.--N--(2,6-Difluorophenyl)-4-(3-pyri- 163-166 dine hydrochloride dinyl)-2-pyrimidinamine100 Ex. 7 3,5-Dimethylphenylguani- .sub.--N--(3,5-Dimethylphenyl)-4-(5-methyl- 133-135 dine hydrochloride 2-thienyl)-2-pyrimidinamine101 Ex. 7 4-Ethylphenylguanidine .sub.--N--(4-Ethylphenyl)-4-(5-methyl-2- 123-125 carbonate thienyl)-2-pyrimidinamine102 Ex. 11 3,4-Dimethylphenylguani- .sub.--N--[(3,4-Dimethylphenyl)methyl]-4- 158-160 dine hydrochloride (2-pyridinyl)-2-pyrimidinamine103 Ex. 7 3,5-Dimethylphenylguani- .sub.--N--(3,5-Dimethylphenyl)-4-(3-methyl- 151-155 dine hydrochloride 2-thienyl)-2-pyrimidinamine104 Ex. 9 3-Methylphenylguanidine .sub.--N--(3-Methylphenyl)-4-(1H--pyrrol-2- 129-130 carbonate yl)-2-pyrimidinamine105 Ex. 8 3-Methylphenylguanidine 4-(5-Methyl-2-furanyl)- .sub.--N--(3-meth- 119-121 carbonate ylphenyl)-2-pyrimidinamine106 Ex. 21 Phenylguanidine carbonate 4-Methyl-6-(5-methyl-2-thienyl)- .sub.--N-- 133-135 phenyl-2-pyrimidinamine107 Ex. 3 4-(Dimethylamino)phenyl- .sub.--N--[4-(Dimethylamino)phenyl]-4-(4- 164-166 guanidine dihydrochloride pyridinyl)-2-pyrimidinamine108 Ex. 3 3-Methoxyphenylguanidine .sub.--N--(3-Methoxyphenyl)-4-(4-pyri- 159-160 hydrochloride dinyl)-2-pyrimidinamine109 Ex. 11 3-Methoxyphenylguanidine .sub.--N--(3-Methoxyphenyl)-4-(2-pyridin- 110-113 hydrochloride yl)-2-pyrimidinamine110 Ex. 11 4-(Dimethylamino)phenyl- .sub.--N--[4-(Dimethylamino)phenyl]-4-(2- 171-174 guanidine dihydrochloride pyridinyl)-2-pyrimidinamine111 Ex. 1 3-Methoxyphenylguanidine .sub.--N--(3-Methoxyphenyl)-4-(3-pyridin- 126-127 hydrochloride yl)-2-pyrimidinamine112 Ex. 1 3,5-Dimethylphenylguani- .sub.--N--(3,5-Dimethylphenyl)-4-(3-pyri- 125-128 dine hydrochloride dinyl)-2-pyrimidinamine113 Ex. 1 4-(Ethoxycarbonyl)phenyl- 4-[[4-(3-Pyridinyl)-2-pyrimidinyl]- 197-202 guanidine hydrochloride amino]benzoic acid, ethyl ester114 Ex. 1 4-(Dimethylamino)phenyl- .sub.--N, .sub.--N--Dimethyl- .sub.--N'--[4-(3-p yridinyl)- 165-166 quanidine dihydrochloride 2-pyrimidinyl]-1,4-benzenediamine115 Ex. 22 Phenylguanidine carbonate 4-(2,5-Dimethyl-3-furanyl)- .sub.--N-- 116-118 phenyl-2-pyrimidinamine116 Ex. 17 4-Ethylphenylguanidine .sub.--N--(4-Ethylphenyl)-4-(3-thienyl)-2- 151-152.5 carbonate pyrimidinamine117 Ex. 22 3-Methylphenylguanidine 4-(2,5-Dimethyl-3-furanyl)- .sub.--N--(3- 144-146 carbonate methylphenyl)-2-pyrimidinamine118 Ex. 22 3,5-Dimethylphenylguani- 4-(2,5-Dimethyl-3-furanyl)- .sub.--N--(3,5- 149-152 dine hydrochloride dimethylphenyl)-2-pyrimidinamine119 Ex. 22 4-Ethylphenylguanidine 4-(2,5-Dimethyl-3-furanyl)- .sub.--N--(4- 93-96 carbonate ethylphenyl)-2-pyrimidinamine120 Ex. 1 3-Dimethylaminophenyl- .sub.--N, .sub.--N--Dimethyl- .sub.--N'--[4-(3-p yridinyl)-2- 123-125 guanidine dihydrochloride pyrimidinyl]-1,3-benzenediamine121 Ex. 11 3-(Ethoxycarbonyl)phenyl- 3-[[4-(2-Pyridinyl)-2-pyrimidinyl[- 156-158 guanidine hydrochloride amino]benzoic acid, ethyl ester122 Ex. 11 3-(Dimethylamin0ophenyl- .sub.--N, .sub.--N--Dimethyl- .sub.--N'--[4-(2-p yridinyl)-2- 109-111 guanidine dihydrochloride pyrimidinyl]-1,3-benzenediamine123 Ex. 1 3-(Ethoxycarbonyl)phenyl- 3-[[4-(3-Pyridinyl)-2-pyrimidinyl]- 95-103 guanidine hydrochloride amino]benzoic acid, ethyl ester124 Ex. 10 4-(Dimethylamino)phenyl- .sub.--N'--[4-(2-Furanyl)-2-pyrimidinyl]- 166-167 guanidine dihydrochloride .sub.--N, .sub.--N--dimethyl-1,4-benzenediamine125 Ex. 4 4-(Dimethylamino)phenyl- .sub.--N, .sub.--N--Dimethyl- .sub.--N'--[4-(2-t hienyl)-2- 174-175 guanidine dihydrochloride pyrimidinyl]-1,4-benzenediamine126 Ex. 22 4-(Dimethylamino)phenyl- .sub.--N'--[4-(2,5-Dimethyl-3-furanyl)-2- 126-129 guanidine dihydrochloride pyrimidinyl]- .sub.--N, .sub.--N--dimethyl-1,4- benzenediamine127 Ex. 19 4-(Dimethylamino)phenyl- .sub.--N, .sub.--N--Dimethyl- .sub.--N'--[4-(3-m ethyl-2- 145-148 guanidine dihydrochloride thienyl)-2-pyrimidinyl]-1,4- benzenediamine128 Ex. 3 3-(Dimethylamino)phenyl- .sub.--N, .sub.--N--Dimethyl- .sub.--N'--[4-(4-p yridinyl)-2- 165-168 guanidine dihydrochloride pyrimidinyl]-1,3-benzenediamine129 Ex. 12 3,5-Dimethylphenylguani- .sub.--N--(3,5-Dimethylphenyl)-4-(2-fur- 155-158 dine anyl)-5-methyl-2-pyrimidinamine130 Ex. 12 4-(Dimethylamino)phenyl- .sub.--N'--[4-(2-Furanyl)-5-methyl-2- 146-148 guanidine dihydrochloride pyrimidinyl] .sub.--N, .sub.--N--dimethyl-1,4- benzenediamine131 Ex. 29 4-(Dimethylamino)phenyl- .sub.--N'--[4-(2-Benzofuranyl)-2-pyrimi- 175-178 guanidine dihydrochloride dinyl]- .sub.--N, .sub.--N--dimethyl-1,4-benzene- diamine132 Ex. 11 2-Guanidinobenzimidazole .sub.--N--[4-(2-Pyridinyl)-2-pyrimidinyl]- 276-279.5 1H--benzimidazol-2-amine132 Ex. 23 Phenylguanidine carbonate 4-Methyl- .sub.--N--phenyl-6-(2-pyridinyl)- 94-98 2-pyrimidinamine134 Ex. 4 3-(Dimethylamino)phenyl- .sub.--N, .sub.--N--Dimethyl- .sub.--N'--[4-(2-t hienyl)-2- 118-120 guanidine dihydrochloride pyrimidinyl]-1,3-benzenediamine135 Ex. 8 3-(Dimethylamino)phenyl- .sub.--N, .sub.--N--Dimethyl- .sub.--N'--[4-(5-m ethyl-2- 126-129 quanidine dihydrochloride furanyl)-2-pyrimidinyl]-1,3-ben- zenediamine136 Ex. 22 3-(Dimethylamino)phenyl- .sub.--N'--[4-(2,5-Dimethyl-3-furanyl)-2- 153-155 guanidine dihydrochloride pyrimidinyl]- .sub.--N, .sub.--N--dimethyl-1,3- benzenediamine137 Ex. 3 4-Aminoacetylphenylguani- .sub.--N--[4-[[4-(Pyridinyl)-2-pyrimidin- 294-296 dine hydrochloride yl]amino]phenyl]acetamide138 Ex. 3 4-(Diethylamino)phenyl- .sub.--N, .sub.--N--Diethyl- .sub.--N'--[4-(4-py ridinyl)- 126-128 guanidine dihydrochloride 2-pyrimidinyl]-1,4-benzenediamine139 Ex. 1 4-(Diethylamino)phenyl- .sub.--N, .sub.--N--Diethyl- .sub.--N'--[4-(3-py ridinyl)- 100-104 guanidine dihydrochloride 2-pyrimidinyl]-1,4-benzenediamine140 Ex. 17 Phenylguanidine carbonate .sub.--N--Phenyl-4-(3-thienyl)-2-pyrimidin- 142-143 amine141 Ex. 11 4-Fluorophenylguanidine .sub.--N--(4-Fluorophenyl)-4-(2-pyridinyl)- 207-209 carbonate 2-pyrimidinamine142 Ex. 11 4-Chlorophenylguanidine .sub.-- N--(4-Chlorophenyl)-4-(2-pyridinyl)- 220-222 carbonate 2-pyrimidinamine143 Ex. 3 4-Methylphenylguanidine .sub.--N--(4-Methylphenyl)-4-(4-pyridinyl)- 197.5-198.5 carbonate 2-pyrimidinamine144 Ex. 31 .sub.--N--[3-(Trifluoromethyl)- 4-(2-Phenothiazine)- .sub.--N--[3-(tri- 240-243 phenyl]guanidine carbon- fluoromethyl)phenyl]-2-pyrimidin- ate amine145 Ex. 31 4-Methoxyphenylguanidine .sub.--N--(4-Methoxyphenyl)-4-(2-pheno- 220-225 carbonate thiazine)-2-pyrimidinamine146 Ex. 31 3,4-Dichlorophenylguani- .sub.--N--(3,4-Dichlorophenyl)-4-(2-pheno- 235-238 dine carbonate thiazine)-2-pyrimidinamine147 Ex. 11 2,4-Dimethylphenylguani- .sub.--N--(2,4-Dimethylphenyl)-4-(2-pyri- 111.5-113.5 dine carbonate dinyl)-2-pyrimidinamine148 Ex. 3 2-Methoxyphenylguanidine .sub.--N--(2-Methoxyphenyl)-4-(4-pyridin- 112-117 carbonate yl)-2-pyrimidinamine149 Ex. 3 2,5-Dimethoxyphenylguani- .sub.--N--(2,5-Dimethoxyphenyl)-4-(4-pyri- 151.5-155.0 dine carbonate dinyl)-2-pyrimidinamine150 Ex. 11 2-Methoxy-5-methylphenyl- .sub.--N--(2-Methoxy-5-methylphenyl)-4-(2- 117-118.5 guanidine carbonate pyridinyl)-2-pyrimidinamine151 Ex. 3 3,4-Dimethylphenylguani- .sub.--N--[(3,4-Dimethylphenyl)methyl]-4- 132-136 dine hydrochloride (4-pyridinyl)-2-pyrimidinamine152 Ex. 29 3-Methylphenylguanidine 4-(2-Benzofuranyl)- .sub.--N--(3-methyl- 143-144 carbonate phenyl)-2-pyrimidinamine153 Ex. 3 3,4-Dimethylphenylguani- .sub.--N--(3,4-Dimethylphenyl)-4-(4-pyri- 169-171.5 dine carbonate dinyl)-2-pyrimidinamine154 Ex. 17 4-Fluorophenylguanidine .sub.--N--(4-Fluorophenyl)-4-(3-thienyl)- 185-187 carbonate 2-pyrimidinamine155 Ex. 31 Phenylguanidine carbonate 4-(10H--Phenothiazin-2-yl)- .sub.--N-- 218-220 phenyl-2-pyrimidinamine156 Ex. 6 4-Ethylphenylguanidine .sub.--N--(4-Ethylphenyl)-4-(1H--indol-3- 209-210 carbonate yl)-2-pyrimidinamine157 Ex. 3 1,1'-Biphenylguanidine .sub.--N--[1,1'-Biphenyl]-4-yl-4-(4-pyri- 203-205 hydrochloride dinyl)-2-pyrimidinamine158 Ex. 3 [4-(1,1-Dimethylethyl)- .sub.--N--[4-(1,1-Dimethylethyl)phenyl]- 181-183 phenyl]guanidine sulfate 4-(4-pyridinyl)-2-pyrimidinamine159 Ex. 11 .sub.--N--Methyl- .sub.--N--phenylguani- .sub.--N--Methyl- .sub.--N--phenyl-4-(2-pyridiny l)- 88-91 dine hydrochloride 2-pyrimidinamine160 Ex. 9 4-Ethylphenylguanidine .sub.--N--(4-Ethylphenyl)-4-(1 .sub.--H--pyrrol- 2- 131-133 carbonate yl)-2-pyrimidinamine161 Ex. 19 Phenylguanidine carbonate 4-(3-Methyl-2-thienyl)- .sub.--N--phenyl- 137-140 2-pyrimidinamine162 Ex. 25 4-Dimethylaminophenyl- .sub.--N, .sub.--N--Dimethyl- .sub.--N'--[4-meth yl-6-(4- 153-154 guanidine dihydrochloride pyridinyl)-2-pyrimidinyl]-1,4- benzenediamine163 Ex. 26 3,5-Dimethylphenylguani- .sub.--N--(3,5-Dimethylphenyl)-4-methyl-6- 136-140 dine hydrochloride (3-pyridinyl)-2-pyrimidinamine164 Ex. 12 .sub.--N--[3-(Trifluoromethyl)-ate 4-(2-Furanyl)-5-methyl- .sub.--N--[3-(tri- 169-171 phenyl]guanidine carbon- fluoromethyl)phenyl]-2-pyrimidin- amine165 Ex. 23 .sub.--N--(3,5-Dimethylphenyl)- .sub.--N--(3,5-Dimethylphenyl)-4-methyl-6- 110-112 guanidine (2-pyridinyl)-2-pyrimidinamine166 Ex. 10 2-Guanidinobenzimidazole .sub.--N--[4-(2-Furanyl)-2-pyrimidinyl]- 306.5-308 1H--benzimidzaol-2-amine167 Ex. 23 .sub.--N--[4-(Dimethylamino)- .sub.--N, .sub.--N--Dimethyl- .sub.--N'--[4-meth yl-6-(2- 145-148 phenyl]guanidine dihydro- pyridinyl)-2-pyrimidinyl]-1,4- chloride benzenediamine168 Ex. 3 4-(1-Imidazolyl)phenyl- .sub.--N--[4-(1 .sub.--H--Imidazol-1-yl)Phenyl]- 4- >320 guanidine dihydrochloride (4-pyridinyl)-2-pyrimidinamine169 Ex. 30 4-(1-Imidazolyl)phenyl- .sub.--N--[4-(1 .sub.--H-Imidazol-1-yl)phenyl]-4 - 134-174 guanidine dihydrochloride (3-pyridinyl)-2-pyrimidinamine (Dec.)170 Ex. 11 .sub.--N--[4-Diethylamino)phen- .sub.--N, .sub.--N--Diethyl-n'-[4-(2-pyridinyl)- 2- 138-139 yl]guanidine dihydro- pyrimidinyl]-1,4-benzenediamine chloride171 Ex. 11 4-(1-Imidazolyl)phenyl- .sub.--N--[4-(1 .sub.--H-Imidazol-1-yl)phenyl]-4 - 204-206 guanidine dihydrochloride (2-pyridinyl)-2-pyrimidinamine172 Ex. 10 4-(1-Imidazolyl)phenyl- 4-(2-Furanyl)- .sub.--N--[4-(1 .sub.--H--imidazol -1- 211-212.5 guanidine dihydrochloride yl)phenyl]-2-pyrimidinamine173 Ex. 12 .sub.--N--[3-Dimethylamino)phen- .sub.--N, .sub.--N--Dimethyl- .sub.--N'--[4-(2-f uranyl)-5- 154-156 yl]guanidine dihydro- methyl-2-pyrimidinyl]-1,3-benzene- 154-156 chloride diamine174 Ex. 21 .sub.--N--[3-Dimethylamino)phen- .sub.--N, .sub.--N--Dimethyl- .sub.--N'-[4-(5-me thyl-2- 130-133 yl]guanidine dihydro- thienyl)-2-pyrimidinyl]-1,3-ben- chloride zenediamine175 Ex. 17 .sub.--N--[4-(Dimethylamino)- .sub.--N, .sub.--N--Dimethyl-n'-[4-(3-thienyl)-2 - 173-174 phenyl]guanidine dihydro- pyrimidinyl]-1,4-benzenediamine chloride176 Ex. 13 .sub.--N--[3-Dimethylamino)phen- .sub.--N, .sub.--N--Dimethyl- .sub.--N'-]4-methy l-6-(4- 200-201 yl]guanidine dihydro- pyridiny)-2-pyrimidinyl]-1,3- chloride benzenediamine177 Ex. 4 4-(1-Imidazolyl)phenyl- .sub.--N--[4-(1 .sub.--H-Imidazol-1-yl)phenyl]-4 - 179-189 guanidine hydrochloride (2-thienyl)-2-pyrimidinamine (Dec.)178 Ex. 19 .sub.--N--(3-Methoxyphenyl)guani- .sub.--N--(3-Methoxyphenyl)-4-(3-methyl-2- 120-123 dine hydrochloride thienyl)-2-pyrimidinamine179 Ex. 30 .sub.--N--[4-(Acetylamino)phen- .sub.--N--[4-[[4-(3-Pyridinyl)-2-pyrimidin- 192-195 yl]guanidine hydrochloride yl]amino]phenyl]acetamide180 Ex. 30 .sub.--N--(4-Benzenesulfonamido)- 4-[[4-(3-Pyridinyl)-2-pyrimidinyl]- 224-225 guanidine hydrochloride amino]benzenesulfonamide181 Ex. 3 .sub.--N--(3-Chlorophenyl)guani- .sub. --N--(3-Chlorophenyl)-4-(4-pyridinyl)- 160-161 dine carbonate 2-pyrimidinamine182 Ex. 30 .sub.--N--(3-Chlorophenyl)guani- .sub.--N--(3-Chlorophenyl)-4-(3-pyridinyl)- 146-148 dine carbonate 2-pyrimidinamine183 Ex. 17 .sub.--N--(3-Methoxyphenyl)guani- .sub.--N--(3-Methoxyphenyl)-4-(3-thienyl)- 142-145 dine hydrochloride 2-pyrimidinamine184 Ex. 4 .sub.--N--(3-Methoxyphenyl)guani- .sub.--N--(3-Methoxyphenyl)-4-(2-thienyl)- 151-153 dine hydrochloride 2-pyrimidinamine185 Ex. 30 .sub.--N--Methyl- .sub.--N--acteylphenyl- .sub.--N--Methyl- .sub.--N--[4-[[4-(3-pyridinyl) -2- 194-197 guanidine hydrochloride pyrimidinyl]amino]phenyl]acetamide186 Ex. 3 .sub.--N--Methyl- .sub.--N--acetylphenyl- .sub.--N--Methyl-n--[4-[[4-pyridinyl)-2- 233-234 guanidine hydrochloride pyrimidinyl]amino]phenyl]acetamide187 Ex. 11 .sub.--N--Methyl- .sub.--N--acetylphenyl- .sub.--N--Methyl- .sub.--N--[4-[[4-(2-pyridinyl) -2- 179-181 guanidine hydrochloride pyrimidinyl]amino]phenyl]acetamide188 Ex. 10 .sub.--N--(3-Methoxyphenyl)guani- 4-(2-Furanyl)- .sub.--N--(3-methoxyphenyl)- 114-116 dine hydrochloride 2-pyrimidinamine189 Ex. 29 .sub.--N--(3-Methoxyphenyl)guani- 4-(2-Benzofuranyl)- .sub.--N--(3-methoxy- 137 dine hydrochloride phenyl)-2-pyrimidinamine190 Ex. 9 .sub.--N--(Ethylphenyl)guanidine .sub.--N--(4-Ethylphenyl)-4-(1-methyl-1H-- 89-91 carbonate pyrrol-2-yl)-2-pyrimidinamine191 Ex. 3 .sub.--N--Acetylphenylguanidine .sub.--N--[4-[[4-(4-Pyridinyl)-2-pyrimi- 294-296 hydrochloride dinyl]amino]phenyl]acetamide192 Ex. 10 .sub.--N, .sub.--N--Dimethylphenylguani- .sub.--N, .sub.--N--Dimethyl- .sub.--N'--[4-(2-f uranyl)-5- 154-156 dine dihydrochloride methyl-2-pyrimidinyl]-1,3-benzene- diamine193 Ex. 30 .sub.--N--Acetylphenylguanidine .sub.--N--[4-[[4-(3-Pyridinyl)-2-pyrimidin- 192-195 hydrochloride yl]amino]phenyl]acetamide194 Ex. 11 Sulfonylaminophenyl- 4-[[4-(2-Pyridinyl)-2-pyrimidinyl]- 274-277 guanidine hydrochloride amino]benzenesulfonamide195 Ex. 11 .sub.--N--Acetylphenylguanidine .sub.--N--[4-[[4-(2-Pyridinyl)-2-pyrimidin- 254-255 hydrochloride yl]amino]phenyl]acetamide196 Ex. 4 3-Methoxyphenylguanidine .sub.--N--(3-Methoxyphenyl)-4-(2-thienyl)- 151-153 hydrochloride 2-pyrimidinamine197 Ex. 30 4-(4-Methylpiperazin-1- .sub.--N--[4-(4-Methyl-1-piperazinyl)- 174-175 yl)phenylguanidine phenyl]-4-(3-pyridinyl)-2-pyrimi- dihydrochloride dinamine198 Ex. 7 3-Methoxyphenylguanidine .sub.--N--(3-Methoxyphenyl)-4-(5-methyl-2- 149-151 hydrochloride thienyl)-2-pyrimidinamine199 Ex. 11 3-Chlorophenylguanidine .sub.--N--(3-Chlorophenyl)-4-(2-pyridinyl)- 164-165 hydrochloride 2-pyrimidinamine200 Ex. 10 4-(4-Methylpiperazine-1- 4-(2-Furanyl)- .sub.--N--[4-(4-methyl-1- 193-195 yl)phenylguanidine piperazinyl)phenyl]-2-pyrimidin- dihydrochloride amine201 Ex. 4 4-(4-Methylpiperazin-1- .sub.--N--[4-(4-Methyl-1-piperazinyl)- 215.5-216.5 yl)phenylguanidine phenyl]-4-(2-thienyl)-2-pyrimidin- dihydrochloride amine202 Ex. 11 4-(4-Methylpiperazin-1- .sub.--N--[4-(4-Methyl-1-piperazinyl)- 192-193 yl)phenylguanidine phenyl]-4-(2-pyridinyl)-2-pyrimi- dihydrochloride dinamine203 Ex. 13 4-(4-Methylpiperazin-1- .sub.--N--[4-(4-Methyl-1-piperazinyl)- 207-209 yl)phenylguanidine phenyl]-4-(4-pyridinyl)-2- dihydrochloride pyrimidinamine204 Ex. 22 3-Methoxyphenylguani- .sub.-- N--(3-Methoxyphenyl)-4-(2,5-dimeth- 124-125 dine hydrochloride yl-3-furanyl)-2-pyrimidinamine205 Ex. 13 3-Fluorophenylguani- .sub.--N--(3-Fluorophenyl)-4-(4-pyridinyl)- 162 dine hydrochloride 2-pyrimidinamine206 Ex. 30 3-Fluorophenylguani- .sub.--N--(3-Fluorophenyl)-4-(3-pyridinyl)- 147-150 dine hydrochloride 2-pyrimidinamine207 Ex. 11 3-Fluorophenylguani- .sub.--N--(3-Fluorophenyl)-4-(2-pyridinyl)- 162-164 dine hydrochloride 2-pyrimidinamine208 Ex. 30 4-Acetylphenylguani- 1-[3-[[4-(3-Pyridinyl)-2-pyrimi- 166-168 dine dinyl]amino]phenyl]ethanone209 Ex. 30 1-(Methylethyl)phenyl- .sub.--N--[4-(1-Methylethyl)phenyl]-4-(3- 124-125 guanidine hydrochloride pyridinyl)-2-pyrimidinamine210 Ex. 30 3-Ethylphenylguanidine .sub.--N--(3-Ethylphenyl)-4-(3-pyridinyl)- 80-88 hydrochloride 2-pyrimidinamine211 Ex. 11 3-Ethylphenylguanidine .sub.--N--(3-Ethylphenyl)-4-(2-pyridinyl)- 101-104 hydrochloride 2-pyrimidinamine212 Ex. 11 3-Benzenesulfonamido- 3-[[4-(2-Pyridinyl)-2-pyrimidinyl]- 223-225 guanidine hydrochloride amino]benzenesulfonamide213 Ex. 30 3-Benzenesulfonamido- 3-[[4-(3-Pyridinyl)-2-pyrimidinyl]- 278-280 guanidine hydrochloride amino]benzenesulfonamide214 Ex. 24 4-(1,1-Dimethylethyl)- .sub.--N--[4-(1,1-Dimethylethyl)phenyl]-4- 150-154 phenylguanidine hydro- (2-thienyl)-2-pyrimidinamine chloride215 Ex. 10 4-(Diethylamino)phenyl- .sub.--N, .sub. --N--Diethyl- .sub.--N'--[4-(2-f uranyl)-2- 132-133 guanidine hydrochloride pyrimidinyl]-1,4-benzenediamine216 Ex. 13 4-Benzenesulfonamido- 3-[[4-(4-Pyridinyl)-2-pyrimidinyl]- 265-264 guanidine hydrochloride amino]benzenesulfonamide217 Ex. 13 4-Acetylaminophenyl- .sub.--N--[3-[[4-(4-Pyridinyl)-2-pyrimi- 267-270 guanidine hydrochloride dinyl]amino]phenyl]acetamide218 Ex. 30 4-Acetylaminophenyl- .sub.--N--[3-[[4-(3-Pyridinyl)-2-pyrimi- 239-241 guanidine hydrochloride dinyl]amino]phenyl]acetamide219 Ex. 11 3-Acetylaminophenyl- .sub.--N--[3-[[4-(2-Pyridinyl)-2-pyrimi- 190-192 guanidine hydrochloride dinyl]amino]phenyl]acetamide220 Ex. 13 3-(1 .sub.--H--Imidazol-1-yl)- .sub.--N--[3-(1 .sub.--H--Imidazol-1-yl)phenyl]- 4- 232-234 phenylguanidine di- (4-pyridinyl)-2-pyrimidinamine hydrochloride221 Ex. 13 4-Acetylamino-3-methyl- .sub.--N--[2-Methyl-4-[[4-(4-pyridinyl)-2- 230-235 phenylguanidine hydro- pyrimidinyl]amino]phenyl]acetamide chloride222 Ex. 21 4-Acetylaminophenyl- .sub.--N--[4-[[4-(5-Methyl-2-thienyl)-2- 227-230 guanidine hydrochloride pyrimidinyl]amino]phenyl]acetamide223 Ex. 30 3-]2-(Diethylaminoeth- .sub.--N--[3-[2-(Diethylamino)ethoxy]phen- 79-82 oxy)phenyl]guanidine yl]-4-(3-pyridinyl)-2-pyrimi- dihydrochloride dinamine224 Ex. 30 2-Methoxyphenylguani- .sub.--N--(2-Methoxyphenyl)-4-(3-pyridin- 99-101 dine carbonate yl0-2-pyrimidinamine225 Ex. 24 4-Acetylaminophenyl- .sub.--N--[4-[[4-(2-Thienyl)-2-pyrimidin- 201-203 guanidine hydrochloride yl]amino]phenyl]acetamide226 Ex. 30 4-Acetylamino-3-methyl- .sub.--N--[2-Methyl-4-[4-(3-pyridinyl)-2- 233-235 phenylguanidine hydro- pyrimidinyl]acetamide chloride227 Ex. 29 4-Diethylaminophenyl- .sub.--N'--]4-(2-Benzofuranyl)-2-pyrimidin- 134-136 guanidine hydrochloride yl]- .sub.--N, .sub.--N--diethyl-1,4-benzenediami ne228 Ex. 12 4-Acetylaminophenyl- .sub.--N--[4-[[4-(2-Furanyl)-2-pyrimidin- 230-232 guanidine hydrochloride yl]amino]phenyl]acetamide229 Ex. 13 4-(Imidazol-1-yl)-3- .sub.--N--[4-(1 .sub.--H--Imidazol-1-yl)-3-(tri- 238-239 (trifluoromethyl)phen- fluoromethyl)phenyl]-4-(4-pyridin- ylguanidine dihydro- yl)-2-pyrimidinamine chloride230 Ex. 11 4-Acetylamino-3-methyl- .sub.--N--[2-Methyl-4-[[4-(2-pyridinyl)-2- 232-234 phenylguanidine hydro- pyrimidinyl]amino]phenyl] acetamide chloride231 Ex. 30 3-(1-Imidazolyl)phenyl- .sub.--N--[3-(1 .sub.--H-Imidazol-1-yl)phenyl]-4 - 137-144 guanidine dihydro- (3-pyridinyl)-2-pyrimidinamine chloride232 Ex. 24 3-(1-Imidazolyl)phenyl- .sub.--N--[3-(1 .sub.--H-Imidazolyl)phenyl]-4-(2 - 183-184.5 guanidine dihydro- thienyl)-2-pyrimidinamine chloride233 Ex. 10 3-(1-Imidazolyl)phenyl- 4-(2-Furanyl)- .sub.--N--[3-(1 .sub.--H-imidazol- 1- 160-168 guanidine dihydro- yl)phenyl]-2-pyrimidinamine chloride234 Ex. 10 3-(Diethylamino)ethoxy- .sub.--N--[3-[2-(Diethylamino)ethoxy]phen- phenylguanidine dihydro- yl]-4-(2-furanyl)-2-pyrimidinamine chloride235 Ex. 10 3-Methylphenylguanidine 4-(2-Furanyl)- .sub.--N--(3-methylphenyl)-2- 195-199 hydrochloride pyrimidinamine, hydrochloride236 Ex. 11 4-(1-Imidazolyl)-3-(tri- .sub.--N--[4-(1 .sub.--H--Imidazol-1-yl)-3-(tri- 2 216-218 fluoromethyl)phenyl- fluoromethyl)phenyl]-4-(2-pyridin- guanidine dihydro- yl)-2-pyrimidinamine chloride237 Ex. 24 3-(Diethylamino)ethoxy- .sub.--N--[3-[2-(Diethylamino)ethoxy]phen- phenylguanidine di- yl]-4-(2-thienyl)-2-pyrimidinamine hydrochloride238 Ex. 10 4-Benzenesulfonamido- 4-[[4-(2-Furanyl)-2-pyrimidinyl]- 255-257 guanidine hydrochloride amino]benzenesulfonamide239 Ex. 21 4-Benzenesulfonamido- 4-[[4-(5-Methyl-2-thienyl)-2- 241-245 guanidine hydrochloride pyrimidinyl]amino]benzenesul- fonamide240 Ex. 17 .sub.--N--Methylacetylamino- .sub.--N--Methyl- .sub.--N--[4-]]4-(3-thienyl)-2 - 150-153 phenylguanidine hydro- pyrimidinyl]amino]phenyl]acetamide chloride241 Ex. 13 3-[4-Methyl-1-pipera- .sub.--N--[3-(4-Methyl-1-piperazinyl)phen- 150-151.5 zinyl]phenylguanidine yl]-4(4-pyridinyl)-2-pyrimidina- hydrochloride mine242 Ex. 10 3-[4-Methyl-1-pipera- 4-(2-Furanyl)- .sub.--N--[3-(4-methyl-1- 134.5-136 zinyl]phenylguanidine piperazinyl)phenyl]-2-pyrimidina- hydrochloride mine243 Ex. 24 3-[4-Methyl-1-pipera- .sub.--N--[3-(4-Methyl-1-piperazinyl)phen- 125-126.5 zinyl]phenylguanidine yl]-4-(2-thienyl)-2-pyrimidinamine hydrochloride244 Ex. 13 2-Dimethylaminopheny- .sub.--N, .sub.--N--Dimethyl- .sub.--N'--[4-(4-p yridinyl)-2- 114-119 guanidine dihydro- pyrimidinyl]-1,2-benzenediamine245 Ex. 13 3-(Diethylamino)ethoxy- .sub.--N--[3-[2-(Diethylamino)ethoxy]phen- 100-103 phenylguanidine di- yl]-4-(4-pyridinyl)-2-pyrimidina- hydrochloride mine246 Ex. 24 3-(Diethylamino)ethoxy- .sub.--N--[4-[2-(Diethylamino)ethoxy]phen- phenylguanidine di- yl]-4-(2-thienyl)-2-pyrimidinamine hydrochloride247 Ex. 24 3-(Dimethylamino)ethoxy- .sub.--N--[4-[2-(Dimethylamino)ethoxy]phen- 96-98 phenylguanidine di- yl]-4-(2-thienyl)-2-pyrimidinamine hydrochloride248 Ex. 17 3-(Dimethylamino)ethoxy- .sub.--N--[4-[2-(Dimethylamino)ethoxy]phen- 83-85 phenylguanidine di- yl]-4-(3-thienyl)-2-pyrimidinamine hydrochloride249 Ex. 21 4-Diethylaminophenyl- .sub.--N, .sub.--N--Diethyl- .sub.--N'--[4-(5-me thyl-2-fur- 118-119 guanidine hydrochloride anyl)-2-pyrimidinyl]-1,4-benzene- diamine250 Ex. 21 3-Methoxyphenylguani- .sub.--N--(3-Methoxyphenyl)-4-(5-methyl-2- -- dine hydrochloride furanyl)-2-pyrimidinamine251 Ex. 13 3-(1 .sub.--H-Imidazol-1-yl)- .sub.--N--[3-(1 .sub.--H-Imidazol-1-yl)phenyl]-4 - 232-239 phenylguanidine di-(4-pyridinyl)-2-pyrimidinamine hydrochloride__________________________________________________________________________
EXAMPLE 252
1-[4-[[4-(3-Pyridinyl)-2-pyrimidinyl]amino]phenyl]ethanone, oxime
A 2.03 mg portion of N-(4-acetylphenyl)-4-(3-pyridinyl)-2-pyrimidinamine was mixed with 210 ml of absolute ethanol and 1.26 g of hydroxylamine hydrochloride. An 18.2 ml portion of 1N sodium hydroxide was added, the mixture was heated at reflux for 2 hours and then evaporated to 1/4 volume. This was cooled, the solid collected, washed with ethanol and water and dried, giving 1.9 g of the desired product as cream colored crystals, mp 239.degree.-241.degree. C.
EXAMPLE 253
1-[4-[[4-(3-Pyridinyl)-2-pyrimidinyl]amino]phenyl]ethanone, O-methyloxime
The procedure of Example 252 was repeated using methoxyamine hydrochloride, giving 1.78 g of the desired product as yellow crystals, mp 163.degree.-167.degree. C.
EXAMPLE 254
N-[1-[4-[[4-(3-Pyridinyl)-2-pyrimidinyl]amino]phenyl]ethyl]formamide
A mixture of 7.25 g of N-(4-acetylphenyl)-4-(3-pyridinyl)-2-pyrimidinamine, 100 ml of formamide and 31 ml, of 98% formic acid was refluxed with stirring overnight. The solvents were then boiled off for 1/2 hour, the reaction cooled and poured into one liter of water. This was extracted with 725 ml of chloroform. The chloroform extract was back washed with 150 ml of water, then dried, filtered and evaporated to a foam. The foam was partitioned between chloroform and water. An equal volume of saturated potassium bicarbonate was added. The organic phase was separated, dried, filtered and evaporated to a foam. This foam was chromatographed on silica gel topped with a thin layer of hydrous magnesium silicate and eluted with chloroform (first four fractions), then with 2% methanol in chloroform (last two fractions). The sixth (final) fraction was evaporated and then crystallized from chloroform-hexane, giving 1.05 g of the desired product as cream colored crystals, mp 118.degree.-121.degree. C.
EXAMPLE 255
N-[4-[2-(Dimethylamino)ethoxy]phenyl]-4-(3-pyridinyl)-2-pyrimidinamine
A 1.10 g portion of dry 4-[[4-(3-pyridinyl)-2-pyrimidinyl]amino]phenol was dissolved in 25 ml of dimethylformamide. A 213 mg portion of sodium hydride (50% in oil) was added, the reaction was sealed and stirred for 45 minutes. A 480 mg portion of 2-dimethylaminoethyl chloride in 2 ml of dimethylformamide was added and the sealed mixture was stirred overnight. The solvent was removed at 60.degree. C. and the residue partitioned between 25 ml of water and 50 ml of ethyl acetate. The aqueous phase was extracted twice with ethyl acetate. The organic phases were combined, washed with 1N sodium hydroxide, dried, filtered and evaporated. The residue was taken up in 20 ml of chloroform, boiled down to 1/3 volume and hexane added to turbidity. The mixture was allowed to stand overnight, giving 400 mg of the desired product as beige crystals, mp 108.degree.-110.degree. C.
EXAMPLE 256
N-[4-[3 -(Dimethylamino)propoxy]phenyl]-4-(3-pyridinyl)-2-pyrimidinamine
A 5.46 g portion of 4-[[4-(3-pyridinyl)-2-pyrimidinyl]amino]phenol was reacted with 3-dimethylaminopropyl chloride by the procedure of Example 255, giving 2.9 g of the desired product, mp 85.degree.-87.degree. C.
EXAMPLE 257
N-[4-[2-(Diethylamino)ethoxy]phenyl]-4-(4-pyridinyl)-2-pyrimidinamine
The procedure of Example 256 was repeated using 4-[[4-(4-pyridinyl)-2-pyrimidinyl]amino]phenol, giving 300 mg of the desired product as yellow crystals, mp 85.degree.-87.degree. C.
EXAMPLE 258
N-[4-[2-(Diethylamino)ethoxy]phenyl]-4-(3-pyridinyl)-2-pyrimidinamine
The procedured of Example 255 was repeated, using 2-diethylaminoethyl chloride, giving 3.45 g of the desired product as yellow crystals, mp 87.degree.-89.degree. C.
EXAMPLE 259
N-[4-[2-(Dimethylamino)ethoxy]phenyl]-4-(4-pyridinyl)-2-pyrimidinamine
The procedure of Example 255 was repeated using 4-[[4-(4-pyridinyl)-2-pyrimidinyl]amino]phenol, giving 1.6 g of the desired product as yellow crystals, mp 120.degree.-122.degree. C.
EXAMPLE 260
N-[4-[2-(Dimethylamino)ethoxy]phenyl]-N',N'-dimethyl-N-[4-(4-pyridinyl)-2-pyrimidinyl]-1,2-ethanediamine
The procedure of Example 259 was repeated. Subsequent crops of crystals gave 0.4 g of the desired product, mp 87.degree.-91.degree. C.
EXAMPLE 261
N-[4-[3-(Dimethylamino)propoxy]phenyl]-4-(4-pyridinyl)-2-pyrimidinamine
A 2.78 g portion of 4-[[4-(4-pyridinyl)-2-pyrimidinyl]amino]phenol and 2.35 g of 3-dimethylaminopropyl chloride was reacted as described in Example 255, giving 850 mg of the desired product, mp 123.degree.-124.5.degree. C.
EXAMPLE 262
[4-[[4-(4-Pyridinyl)-2-pyrimidinyl]amino]phenoxy]acetic acid, ethyl ester
A mixture of 5.58 g of 4-[[4-(4-pyridinyl)-2-pyrimidinyl]amino]phenol was reacted with ethyl bromo acetate as described in Example 255, giving 1.8 g of the desired product as yellow crystals, mp 109.degree.-111.degree. C.
EXAMPLE 263
N-(4-Methoxyphenyl)-N-methyl-4-(3-pyridinyl)-2-pyrimidinamine
A 2.78 g portion of N-(4-methoxyphenyl)-4-(3-pyridinyl)-2-pyrimidinamine was dissolved in 30 ml of dimethylformamide. A 528 mg portion of sodium hydride (50% in oil) was added, the reaction sealed and stirred for 45 minutes. A solution of 1.70 g of methyl iodide in 2 ml of dimethylformamide was added, the sealed mixture was stirred overnight and the solvent removed. The residue was partitioned between water and chloroform. The organic phase was dried, filtered and evaporated. The residue was crystallized from ether-hexane giving 1.4 g of the desired product as yellow crystals, mp 88.degree.-90.degree. C.
EXAMPLE 264
N-(4-Methoxyphenyl)-N-methyl-4-(4-pyridinyl)-2-pyrimidinamine
The procedure of Example 263 was repeated using N-(4-methoxyphenyl)-4-(4-pyridinyl)-2-pyrimidinamine, giving 510 mg of the desired product as yellow crystals, mp 124.degree.-126.degree. C.
EXAMPLE 265
N-[2-(Diethylamino)ethyl]-4-[[4-(3-pyridinyl)-2-pyrimidinyl]amino]benzamide
A 1.55 ml portion of diethylethylenediamine was added to a solution of 0.01 mole of 4-[[4-(3-pyridinyl)-2-pyrimidinyl]amino]benzoic acid chloride in 50 ml of 1,2-dimethoxyethane. A 10 ml portion of triethylamine was added and the mixture was stirred for 2 hours. The solid was collected, washed with water and recrystallized from absolute ethanol, giving 1.22 g of the desired product, mp 148.degree.-150.degree. C.
EXAMPLE 266
N-Methyl-4-[[4-(3-pyridinyl)-2-pyrimidinyl]amino]benzamide
A 5.85 g portion of 4-[[4-(3-pyridinyl)-2-pyrimidinyl]amino]benzoic acid in 30 ml of thionyl chloride was refluxed on a steam bath for one hour, then evaporated to dryness. The residue was boiled with dimethoxyethane, then cooled and the solid recovered and washed with ether, giving 6.90 g of 4-[[4-(3-pyridinyl)-2-pyrimidinyl]amino]benzoic acid chloride.
A 6.03 g portion of the above acid chloride was suspended in 25 ml of ethanol and 10 ml of 25% aqueous methyl amine was added. The resulting solid was collected, taken up in hot 2-methoxyethanol, cooled and the solid collected, giving 3.35 g of the desired product, mp 254.degree.-257.degree. C.
EXAMPLE 267
4-[[4-(3-Pyridinyl)-2-pyrimidinyl]amino]benzoic acid
To a solution of 19.89 g of 4-[[4-(3-pyridinyl)-2-pyrimidinyl]amino]benzoic acid, ethyl ester in 200 ml of 3A ethanol was added 12.5 ml of 10N sodium hydroxide. This mixture was refluxed on a steam bath for 3 hours and then allowed to evaporate. The residue was taken up in water and treated with 10.4 ml of concentrated hydrochloric acid. The resulting solid was collected and dried, giving 18.11 g of the desired product, mp 311.degree.-317.degree. C.
EXAMPLE 268
[4-[[4-(4-Pyridinyl)-2-pyrimidinyl]amino]phenoxy]acetic acid
An 800 mg portion of [4-[[4-(4-pyridinyl)-2-pyrimidinyl]amino]phenoxy]acetic acid, ethyl ester was dissolved in 100 ml of ethanol and 10.7 ml of 1N sodium hydroxide was added. The mixture was stirred for 2 hours, the solvent removed and the residue dissolved in 5 ml of water. The pH was adjusted to 7.0 with 1N hydrochloric acid and the solid collected, washed with water and dried. The solid was recrystallized from dimethylformamide-ethanol, giving 600 mg of the desired product as yellow crystals, mp 308.degree.-310.degree. C.
EXAMPLE 269
4-[2-[(4-Methoxyphenyl)amino]-4-pyrimidinyl]-1-methylpyridinium iodide
A 2.0 g portion of N-(4-methoxyphenyl)-4-(4-pyridinyl-2-pyrimidinamine was dissolved in 550 ml of absolute ethanol and filtered. To this was added 10 ml of iodomethane. The reaction was heated on a steam bath for 4 hours. Another 10 ml of iodomethane was added and refluxing was continued overnight. The mixture was cooled, the solid collected, washed with ethanol and dried, giving 2.2 g of the desired product as purple crystals, mp 282.degree.-284.degree. C.
EXAMPLE 270
4-[[4-(3-Pyridinyl)-2-pyrimidinyl]amino]phenol
A 25.0 g portion of N-(4-methoxyphenyl)-4-(3-pyridinyl)-2-pyrimidinamine was dissolved in 200 ml of 48% hydrobromic acid and stirred overnight under an argon atmosphere. The mixture was then heated on a steam bath for 7 hours, cooled overnight and evaporated at 60.degree. C. The residue was basified with 200 ml of saturated potassium bicarbonate solution and stirred for 1.5 hours. The solid was collected, washed with water, dried and recrystallized from hot absolute ethanol, giving 19.1 g of the desired product, mp 223.degree.-225.degree. C.
EXAMPLE 271
4-[[4-(4-Pyridinyl)-2-pyrimidinyl]amino]phenol
The procedure of Example 270 was repeated using N-(4-methoxyphenyl)-4-(4-pyridinyl)-2-pyrimidinamine, giving 3.0 g of the desired product as yellow crystals, mp 268.degree.-270.degree. C.
EXAMPLE 272
N-[4-(2-Propenyloxy)phenyl]-4-(3-pyridinyl)-2-pyrimidinamine
A 2.73 g portion of dry 4-[[4-(3-pyridinyl)-2-pyrimidinyl]amino]phenol was dissolved in 50 ml of dry dimethylformamide. A 528 mg portion of sodium hydride (50% in oil) was added, the reaction was sealed and stirred for 45 minutes. A solution of 1.33 g of allyl bromide in 10 ml of dimethylformamide was added, the sealed mixture was stirred overnight and then evaporated at 80.degree. C. The residue was partitioned between water and chloroform. The organic phase was separated, dried and filtered. The filtrate was evaporated and the residue crystallized from chloroform-hexane, giving 1.7 g of the desired product as yellow crystals, mp 105.degree.-108.degree. C.
EXAMPLE 273
N-(4-Ethylphenyl)-4-(4-pyridinyl)-2-pyrimidinamine, pyridine-1-oxide
A mixture of 2.76 g of N-(4-ethylphenyl)-4-(4-pyridinyl)-2-pyrimidinamine and 3.45 g of m-chloroperbenzoic acid in 100 ml of dichloromethane was stirred at room temperature for 20 hours. The mixture was washed three times with an aqueous saturated solution of sodium bicarbonate and a small amount of saturated saline. The organic layer was dried over magnesium sulfate, filtered through diatomaceous earth, then evaporated in vacuo to give a gelatenous solid. The solid was slurried with 50 ml of dichloromethane and filtered. The solid was washed with a small amount of dichloromethane and air dried to give 500 mg of the product. Recrystallization from absolute methanol gave 460 mg of the desired product, mp 223.degree.-225.degree. C.
EXAMPLE 274
N-Phenyl-4-(4-pyridinyl)-2-pyrimidinamine, dihydrochloride
A 2.0 g amount of N-phenyl-4-(4-pyridinyl)-2-pyrimidinamine was dissolved in 70 ml of dichloromethane with warming. The solution was cooled to room temperature, then hydrogen chloride gas was bubbled in to give a brick red precipitate. The mixture became very thick and more dichloromethane was added. The precipitate was collected, air dried, then dried in vacuo and gave 2.63 g of the desired product as red-orange crystals, mp 259.degree.-262.degree. C.
EXAMPLE 275
N-[4-(4-Pyridinyl)-2-pyrimidinyl]-1,4-benzenediamine, hydrochloride
A 2.85 g amount of N-[4-[[4-(4-pyridinyl)-2-pyrimidinyl]amino]phenyl]acetamide was added to a mixture of 10 ml of concentrated hydrochloric acid and 10 ml of water. The reaction mixture was heated at reflux for 90 minutes, then evaporated in vacuo to obtain a solid. The solid was recrystallized from 3A ethanol/water and gave 2.31 g of the desired product as a yellow crystalline solid, mp 292.degree.-295.degree. C.
Additional hydrochloride salts listed in Examples 276 to 287 in Table V were obtained from the corresponding base compound by following procedures similar to those described in Examples 274 and 275 and employing various other solvents such as isopropyl alcohol, ethanol, ether and the like.
TABLE V______________________________________Ex Compound MP .degree.C.______________________________________276 4-(3-Pyridinyl)- --N--[3-trifluoromethyl)- 220-223 phenyl]pyrimidinamine, hydrochloride277 --N, --N--Dimethyl- --N'--[4-(3-pyridinyl)-2-pyrim- 239-245 idinyl]-1,4-benzenediamine, trihydrochloride278 --N--[4-[2-(Diethylamino)ethoxy]phenyl]-4- 115-150 (3-pyridinyl)-2-pyrimidinamine, hydrochlo- (dec) ride279 --N, --N--Dimethyl- --N'--[4-(2-pyridinyl)-2-(pyrimi- 204-213 dinyl)]-1,3-benzenediamine, dihydrochloride280 --N, --N--Dimethyl- --N'--[4-(2-pyridinyl)-2-pyrimi- 202-205 dinyl]-1,3-benzenediamine, trihydrochloride281 --N, --N--Dimethyl- --N'--[4-(4-pyridinyl)-2-pyrimi- 178-184 dinyl]-1,3-benzenediamine, dihdrochloride282 --N, --N--Dimethyl- --N'--[4-(2-pyridinyl)-2-pyrimi- 229-234 dinyl]-1,4-benzenediamine, dihydrochloride283 --N, --N--Dimethyl- --N'--[4-(4-pyridinyl)-2-pyrimi- 232-235 dinyl]-1,4-benzenediamine, trihydrochloride284 --N--[4-(1-Aminoethyl)phenyl]-4-(3-pyridinyl)- 2-pyrimidinamine, trihydrochloride285 --N--[3-(1 --H--Imidazol-1-yl)phenyl]-4-(2-pyri- 232.5-234 dinyl)-2-pyrimidinamine, hydrochloride286 --N--[3-(1 --H--Imidazol-1-yl)phenyl]-4-(4-pyri- 259-266 dinyl)-2-pyrimidinamine, hydrochloride287 4-(2-Furanyl)- --N--[3-(4-methyl-1-piperazin- 259-263 yl)phenyl]-2-pyrimidinamine, hydrochloride______________________________________
EXAMPLE 288
N-Phenyl-4-(4-pyridinyl)-2-pyrimidinamine, sulfate
A 2.48 g amount of N-phenyl-4-(4-pyridinyl)-2-pyrimidinamine was dissolved in 120 ml of absolute ethanol with heating, then a solution of 1.02 g of concentrated sulfuric acid in 25 ml of ethanol was added dropwise with stirring. The mixture turned orange then a yellow precipitate formed. The mixture was chilled, the preciptate was collected, by filtration, washed with cold ethanol then with ether, and air dried to give 2.73 g of yellow-orange crystals.
The preceding compound was dissolved in a small amount of water, then a saturated aqueous solution of sodium bicarbonate was added to pH 8.0 to yield a light yellow precipitate. The precipitate was collected, washed with water and dried in vacuo. A 2.25 g portion this material was recrystallized from about 200 ml of absolute methanol in the cold. The product was collected, washed with absolute ethanol and dried in vacuo to give 1.75 g of the desired product as orange cyrstals, mp 233.degree.-235.degree. C.
Additional sulfate salts which were prepared from the corresponding base compound in the manner described hereinabove are listed as Examples 289 to 300 in Table VI.
TABLE VI______________________________________Ex Compound MP .degree.C.______________________________________289 4-(2-Pyridinyl)- --N--[3-trifluoromethyl)- 208-211 phenyl]-2-pyrimidinamine, sulfate290 --N--(3-Methylphenyl)-4-(2-thienyl)-2-pyrimi- 207.5-210 dinamine, sulfate291 4-(2-Furanyl)- --N--(3-methylphenyl)-2-pyrimi- 187-193 dinamine sulfate292 4-(4-Pyridinyl)- --N--[3-(trifluoromethyl)phen- 250-253 yl)]-2-pyrimidinamine, sulfate293 --N--(4-Methoxyphenyl)-4-(3-pyridinyl)-2- 103-123 pyrimidinamine, sulfate294 --N--Phenyl-4-(3-pyridinyl)-2-pyrimidinamine, 167-187 sulfate295 4-(3-Pyridinyl)- --N--[3-trifluoromethyl)phen- 196-199 yl]-2-pyrimidinamine, sulfate296 --N--(3,5-Dimethylphenyl)-[4-(3-pyridinyl)-2- 209-214 pyrimidinamine, sulfate297 --N--(3,5-Dimethylphenyl)-4-(2-pyridinyl)-2- 216-218 pyrimidinamine, sulfate298 --N--(3,5-Dimethylphenyl)-4-methyl-6-(5-methyl- 232-234 2-thienyl)-2-pyrimidinamine, sulfate299 4-(2-Furanyl)-5-methyl- --N--phenyl-2-pyrimi- 140-144 dinamine, sulfate300 --N, --N--Dimethyl- --N'--[4-(4-pyridinyl)-2-pyrimi- 204-211 dinyl]-1,3-benzenediamine, sulfate______________________________________
EXAMPLE 301
N-Phenyl-4-(4-pyridinyl)-2-pyrimidinamine, phosphate
A 2.0 g amount of N-phenyl-4-(4-pyridinyl)-2-pyrimidinamine was dissolved in 100 ml of ethanol with heating. The solution was allowed to cool to room temperature, then a solution of 2.07 g of phosphoric acid in 25 ml of ethanol was added with stirring. The mixture was chilled for several hours, then the precipitate which formed was collected by filtration, washed twice with cold ethanol and dried in vacuo for 16 hours to give 3.43 g of the desired product as orange crystals, mp 210.5.degree.-212.5.degree. C.
Additional phosphate salts which were prepared from the corresponding base compound in the manner described hereinabove are listed as Examples 302 to 305 in Table VII.
TABLE VII______________________________________Ex Compound MP .degree.C.______________________________________302 --N--(3,5-Dimethylphenyl)-4-(3-pyridinyl)-2- 190-192 pyrimidinamine, phosphate303 --N--(4-Methoxyphenyl)-4-(3-pyridinyl)-2- 185-188 pyrimidinamine, phosphate304 --N--Phenyl-4-(3-pyridinyl)-2-pyrimidinamine 176-179 phosphate305 --N--(3,5-Dimethylphenyl)-4-(2-pyridinyl)-2- 199-202 pyrimidinamine, phosphate______________________________________
EXAMPLE 306
N-Phenyl-4-(4-pyridinyl)-2-pyrimidinamine, (Z)-2-butenedioate (1:1)
A mixture of 4.97 g of N-phenyl-4-(4-pyridinyl)-2-pyrimidinamine and 2.55 g of maleic acid was dissolved in hot 2-methoxyethanol. Cooling gave 4.15 g of the desired product as an orange crystalline solid, mp 211.degree.-214.degree. C.
EXAMPLE 307
N-Phenyl-4-(4-pyridinyl)-2-pyrimidinamine, dinitrate
A 2.0 g amount of N-phenyl-4-(4-pyridinyl)-2-pyrimidinamine was dissolved in 100 ml of ethanol with heating. The solution was allowed to cool to room temperature, then a solution of 1.5 ml of concentrated nitric acid in 25 ml of ethanol was added with stirring to give a red-orange precipitate. The mixture was allowed to stand 30 minutes at room temperature, then was chilled for several hours. The solid was collected, washed with cold absolute ethanol and air dried to give 2.80 g of the desired product as red-orange crystals, mp 167.degree.-169.degree. C. (dec.).
EXAMPLE 308
N-Phenyl-4-(4-pyridinyl)-2-pyrimidinamine, compound with 2-hydroxy-1,2,3-propanetricarboxylate (2:1)
A mixture of 4.97 of N-phenyl-4-(4-pyridinyl)-2-pyrimidinamine and 4.62 g of citric acid was dissolved in hot absolute ethanol. Cooling gave 6.14 g of the product of the example as a yellow crystalline solid, mp 155.degree.-157.degree. C.
EXAMPLE 309
Oxo[phenyl[4-(4-pytridinyl)-2-pyrimidinyl]amino]acetic acid, ethyl ester
A 4.08 g portion of 2-phenylamino-4-(4-pyridinyl)pyrimidine was dissolved in 20 ml of dimethylformamide. A 5 g portion of 50% sodium hydride in oil was added using 10 ml of dimethylformamide as a wash. When bubbling ceased, a solution of 2.23 ml of ethyl oxalyl chloride in 10 ml of dimethylformamide was added dropwise. Chloroform and aqueous 10% potassium bicarbonate were added. The organic layer was separated, dried, filtered and evaporated giving the desired product.
EXAMPLE 310
N-[4-(2-Pyridinyl)-2-pyrimidinyl]-1,4-benzenediamine, dihydrochloride
A 12.86 g portion of N-[4-[[4-(2-pyridinyl)-2-pyrimidinyl]amino]phenyl]acetamide in a mixture of 40 ml of water and 40 ml of concentrated hydrochloric acid was refluxed for 30 minutes and then cooled. The solid was collected and dried, giving 10.84 g of the desired product, mp 285.degree.-288.degree. C.
Following the procedure of this Example, and using as starting materials the products of the indicated examples, the products of Examples 311-322 in Table VIII were derived.
TABLE VIII______________________________________ Start- ing Mate-Ex. rial Product MP .degree.C.______________________________________311 Ex. --N--Methyl- --N'--[4-(3-pyridinyl)-2- 164-166 185 pyrimidinyl]-1,4-benzenediamine312 Ex. --N--Methyl- --N'--[4-(2-pyridinyl)-2- 110-112 187 pyrimidinyl]-1,4-benzenediamine313 Ex. --N--[4-(3-Pyridinyl)-2-pyrimidinyl]- 279-284 218 1,3-benzenediamine, dihydrochloride314 Ex. --N--[4-(4-Pyridinyl)-2-pyrimidinyl]- 199-202 217 1,3-benzenediamine315 Ex. 2-Methyl- --N--[4-(4-pyridinyl)-2- 297-304 221 pyrimidinyl]-1,4-benzenediamine, dihydrochloride316 Ex. --N--[4-(2-Pyridinyl)-2-pyrimidinyl]- 153-156 219 1,3-benzenediamine317 Ex. --N--[3-(1-Aminomethyl)phenyl]-4-(3- 230(dec.) 182 pyridinyl)-2-pyrimidinamine318 Ex. --N--[4-(5-Methyl-2-thienyl)-2-pyrimi- 284-287 222 dinyl]-1,4-benzenediamine, dihydro- chloride319 Ex. --N--[4-(2-Furanyl)-2-pyrimidinyl]-1,4- 261-266 228 benzenediamine, dihydrochloride320 Ex. 2-Methyl- -- N--[4-(3-pyridinyl)-2-pyrimi- 176-178 226 dinyl]-1,4-benzenediamine321 Ex. 2-Methyl- --N--[4-(2-pyridinyl)-2-pyrimi- 196-198 230 dinyl]-1,4-benzenediamine322 Ex. --N--[4-(4-Pyridinyl)-2-pyrimidinyl]- 192-193.5 191 1,4-benzenediamine______________________________________
EXAMPLE 323
2-[1-[4-[[4-(3-Pyridinyl)-2-pyrimidinyl]amino]phenyl]ethylidene]hydrazinecarboxamide
A 2.9 g portion of 1-[3-[[4-(3-pyridinyl)-2-pyrimidinyl]amino]phenyl]ethanone was mixed with 1.23 g of semicarbazide hydrochloride in 200 ml of absolute ethanol and 1.10 ml of 10N sodium hydroxide was added. This mixture was refluxed overnight, then cooled to room temperature and the solid collected and washed with ethanol, water and ethanol. The solid was recrystallized from dimethylsulfoxide/ethanol, giving 2.9 g of the desired product, mp 256.degree.-258.degree. C.
EXAMPLE 324
N-[4-[2-[bis(1-Methylethyl)amino]ethoxy]phenyl]-4-(3-pyridinyl)-2-pyrimidinamine
A 2.64 g portion of 4-[[4-(3-pyridinyl)-2-pyrimidinyl]amino]phenol was dissolved in 60 ml of dimethylformamide by warming on a steam bath and then cooled. A 2.0 g portion of diisopropylaminoethyl chloride hydrochloride was added and dissolved with stirring. A 20 ml portion of 5N sodium hydroxide was added dropwise over 5 minutes, then 5 ml of water was added and the mixture was stirred for 20hours. The mixture was then heated on a steam bath for 30 minutes, allowed to stand 48 hours and then evaporated. The residual gum was purified by flash dry column chromatography on silica gel eluting fractions 1-3 with methanol and fractions 4-6 with 1% methanol in chloroform. Fractions 4-6 were combined and evaporated, giving 500 mg of the desired product.
EXAMPLE 325
.alpha.-Methyl-4-[[4-(3-pyridinyl)-2-pyrimidinyl]amino]benzenemethanol
A 1.45 g portion of 1-[3-[[4-(3-pyridinyl)-2-pyrimidinyl]amino]phenyl]ethanone was dissolved with stirring in 220 ml of ethanol. A 125 mg portion of sodium borohydride was added and stirring continued for 3 hours. A 63 mg portion of sodium borohydride was added and stirring continued overnight. A 2 ml portion of glacial acetic acid was added and the mixture evaporated. The solid was triturated with water, dried and recrystallized from 30 ml of ethanol giving 710 mg of the desired product, mp 145.degree.-147.degree. C.
EXAMPLE 326
N-[1-[3-[[4-(3-Pyridinyl)-2-pyrimidinyl]amino]phenyl]ethyl]formamide
A mixture of 2.9 g of 1-[3-[[4-(3-pyridinyl)-2-pyrimidinyl]amino]phenyl]ethanone, 40 ml of formamide and 13 ml of concentrated formic acid was refluxed for 15 hours, then cooled and evaporated. The residue was partitioned between unsaturated aqueous potassium bicarbonate and chloroform. The organic phase was separated, dried, filtered and evaporated. The residue was chromatographed on silica gel, eluting 125 ml fractions, fractions 1-4 with chloroform and fractions 5-7 with 2% methanol in chloroform. Fractions 5-7 were combined and evaporated, giving 1.25 g of the desired product as a yellow foam.
EXAMPLE 327
2-[[4-(3-Pyridinyl)-2-pyrimidinyl]amino]phenol
A mixture of 35 g of N-(2-methoxyphenyl)-4-(3-pyridinyl)-2-pyrimidinamine in 200 ml of 47% aqueous hydrobromic acid was refluxed for 7 hours and then evaporated. The residue was mixed with saturated aqueous potassium bicarbonate and allowed to stand overnight, then filtered. The filtrate was concentrated, giving 3.5 g of the desired compound, mp 166.degree.-169.degree. C.
EXAMPLE 328
N-[3-(1H-Imidazol-1-yl)phenyl]-4-(2-pyridinyl)-2-pyrimidinamine
A solution of 250 ml of 2-acetylpyridine and 500 ml of N,N-dimethylformamide dimethyl acetal was heated on a steam bath for 6 hours. After concentrating the reaction solution under vacuum, 1 liter of hexane was added to the part crystalline residue. The product was collected as small crystalline particles which were washed with an additional liter of hexane. Air drying was followed by drying at 45.degree. C. under vacuum, leaving 350.7 g of 3-dimethylamino-1-(2-pyridinyl)-2-propen-1-one.
A mixture of 289.0 g of imidazole, 292 g of potassium carbonate, 3 liters of dimethyl sulfoxide, and 300.0 g of 1-fluoro-3-nitrobenzene was stirred and heated for 25.5 hours between 105.degree.-110.degree. C. Then the reaction was poured into 6 liters of water and cooled in the refrigerator over the weekend. The crystalline product was collected and washed with 1 liter of water. Air drying gave 357.6 g of solid. The solid was taken up in 2.4 liters of ethyl acetate and the hot solution passed through hydrous magnesium silicate. After boiling the filtrate down to 1.5 liters, it was cooled to give a precipitate which was collected and washed with 200 ml of ethylacetate, to leave 151.7 g of off-white crystals. After evaporating the mother liquor to dryness, the residue was recrystallized from 350 ml of ethyl acetate to give 59.7 g more product. The mother liquor from the second fraction was evaporated and the residual material recrystallized twice from ethyl acetate to give 30.9 g more product. Total product, 242.3 g of 1-(3-nitrophenyl)-1H-imidazole.
In a Parr hydrogenation bottle was placed 75.00 g of 1-(3-nitrophenyl)-1H-imidazole, 0.70 g platinum oxide, and 250 ml of ethanol. Shaking of this mixture in a Parr hydrogenation apparatus was continued until no more hydrogen was taken up. This process was repeated with 76.33 g of the imidazole, 1.0 g of platinum oxide and 250 ml of ethanol and again with 90.4 g of the imidazole, 1.0 g of platinum oxide and 240 ml of ethanol, until a total of 241.63 g had been reduced. For each batch the catalyst was filtered off and the solvent was removed under vacuum; and then the residues were combined to give 207.2 g of gray crystalline amine. Next the amine was recrystallized from 530 ml of 2-propanol. After collecting the product, it was washed with 200 ml of 2-propanol, and dried, under vacuum, to give 156.4 g of 3-(1H-imidazol-1-yl)benzamine.
A solution of 43.3 g of hydrogen chloride in 290 ml of ethanol was added to 189.0 g of 3-(1H-imidazol-1-yl)benzamine in a 2 liter Erlenmeyer flask. Then 104.7 g of cyanamid was added. The mixture was cautiously warmed in a water bath to an internal temperature of 83.degree. C. over 25 minutes. When no exotherm had been noted, the flask was placed inside the steam bath and heated for 2 hours. A final temperature of 97.degree. C. was achieved. The resulting brown syrup which was [3-(1H-imidazol-1-yl)phenyl]quanidine, monohydrochloride, was used in the next reaction without further purification.
A mixture of 164 g of potassium carbonate, 209.1 g of 3-dimethylamino-1-(2-pyridyl)-2-propen-1-one, 1.187 mole of crude [3-(1H-imidazole-1-yl)phenyl]guanidine monohydrochloride, and 1 liter of methoxyethanol was stirred and heated under very gentle reflux. A dry-ice condenser filled with water was used to prevent plugging by the dimethylammonium carbonate which is given off by the reaction. The reaction was stopped after 26.5 hours and permitted to stand overnight. A heavy precipitate had formed which was collected as A and washed with 100 ml of ether. The filtrate was concentrated under vacuum as B. Both A and B were triturated with 1.5 liters of water. Then A was washed with 300-400 ml of ethanol, followed by 100 ml of ether to leave, on drying, 172.9 g of gray solid, mp 200.degree.-202.degree. C. Recrystallization of B from 150 ml of 2-propanol gave a black solid, C. Next, a classical fractional recrystallization was carried out using methoxyethanol as the solvent. In the final stages, a large amount of charcoal was added to remove color. In this fashion two main fractions were obtained D, 79.0 g of yellow crystals, mp 204.5.degree.-205.5.degree. C., and E, 18.05 g of yellow crystals, mp 204.degree.-204.5.degree. C. The yield of D plus E was 26% of the desired product.
EXAMPLE 329
1-(2-Chloroethoxy)-3-nitrobenzene
A mixture of 6.96 g. of m-nitrophenol, 100 ml. of 2-butanone, 6.9 g. of potassium carbonate, and 11.74 g. of 2 chloroethyl-tosylate was stirred and heated under reflux for 24 hours. After cooling to room temperature, the salts were filtered off and the filtrate concentrated under vacuum. The residue crystallized on seeding and was recrystallized from carbon tetrachloride to give 8.3 g. of product, m.p. 54.5.degree.-57.degree. C.
EXAMPLE 330
1-[2-(3-Nitrophenoxy)ethyl]-1H-imidazole
After dissolving 3.74 g. of imidazole in 60 ml. of dry N,N-dimethylformamide, 1.78 g. of 50% sodium hydride in oil was added. When the effervescence had stopped (circa 1 hr.), 7.35 g. of 1-(2-chloroethoxy)-3-nitrobenzene was added. After stirring overnight, the reaction was concentrated under vacuum. Water was added to the residue and the product was extracted into chloroform. The product was extracted out of the chloroform layer with dilute hydrochloric acid. Next, the aqueous acid layer was neutralized with potassium carbonate and the oily product extracted into chloroform. Upon drying the chloroform extract with sodium sulfate, it was concentrated under vacuum to an oil which crystallized on standing. Recrystallization from isopropyl acetate gave 6.12 g. of product as the monohydrate, m.p. 52.5.degree.-55.5.degree. C.
EXAMPLE 331
3-[2-(1H-Imidazol-1-yl)ethoxy]benzamine
Using a Parr hydrogenator, 5.00 g. of 1-[2-(3-nitrophenoxy)ethyl]-1H-imidozole in 100 ml. of ethanol and 0.2 g. of platinum oxide was hydrogenated until the hydrogen uptake stopped. The catalyst was filtered off and the filtrate concentrated under vacuum. Several recrystallizations from isopropyl acetate gave 2.8 g. of amine, m.p. 74.degree.-76.5.degree. C.
EXAMPLE 332
[3-[2-(1H-Imidazol-1-yl)ethoxy]phenyl]-guanidine Dihydrochloride
To a solution of 1.7 g. of hydrogen chloride in 50 ml. of ethanol was added 4.70 g. of 3-[2-(1H-imidazol-1-yl)ethoxy]benzamine in 10 ml. of ethanol. After concentration under vacuum a foam was obtained which gradually crystallized. Next 1.95 g. of cyanamid and 20 ml. of ethanol were added and the mixture heated cautiously, first in a water bath, then directly in a steam bath for a total of 5 hours. A light brown oily guanidine resulted, which was used without purification.
EXAMPLE 333
3-[2-(4-Morpholinyl)ethoxy]-benzenamine
N-[2-Chloroethyl]morpholine hydrochloride, 80 g., was partitioned between 5N sodium hydroxide and methylene chloride. After drying the organic layer over magnesium sulfate, the solvent was removed under reduced pressure to leave 65 g. of free amine.
To 36.01 g. of m-aminophenol dissolved in 325 ml. of N,N-dimethylformamide, 16.3 g. of 50% sodium hydride in oil was added. The reaction was stirred for 1 hour, until the effervescence stopped; then 57 g. of N-(2-chloroethyl)morpholine, from above, was added. After stirring overnight, the mixture was heated on a steam bath for 1/2 hr., then concentrated under vacuum. The residue was taken up in 300 ml. of 2N hydrochloric acid and washed twice with ether. After basifying with 10N sodium hydroxide, the product was extracted into ether, dried (magnesium sulfate), filtered through hydrous magnesium silicate and evaporated to a brown oil. Distillation gave 34.0 g. of a golden oil, b.p. 165.degree.-180.degree. C./0.45 mm.
EXAMPLE 334
[3-[2-(4-Morpholinyl)ethoxy]phenyl]guanidine monohydrochloride
Prepared from 3-[2-(4-morpholinyl)ethoxy]-benzamine by the method of Example 332.
EXAMPLE 335
1-(Bromoacetyl)-4-methylpiperazine monohydrochloride
A solution of 10.0 g. of 1-methyepiperazine in 150 ml of chloroform was cooled in a water bath while 17.3 g. of bromoacetyl chloride in 150 ml. of chloroform was added dropwise, with stirring, over 1/2 hour. A calcium chloride tube protected the reaction from moisture. After stirring overnight, the precipitate was collected and washed with chloroform. The crude product was dried under vacuum at 50.degree. and used as such.
EXAMPLE 336
1-[(4-Aminophenoxy)acetyl]-4-methylpiperazine
Prepared from p-aminophenol and 1-(bromoacetyl)-4-methylpiperazine by the method of Example 333 to give a product of m.p. 71.degree.-73.degree. C.
EXAMPLE 337
1-[[4-[(Aminoiminomethyl)amino]phenoxy]acetyl]-4-methylpiperazine Dihydrochloride
Prepared from 1-[(4-aminophenoxy)acetyl]-4-methylpiperazine by the method of Example 332.
TABLE IX______________________________________ Acry- loyl PhenylguanidineEx. Source precurser Product Mp .degree.C.______________________________________338 Ex. 11 [3-[2-(1H--Imidazol- N--[3-[2-(1H-- 149- 1-yl)-ethoxy]- Imidazol-1-yl)- 151.5 phenyl]guanidine ethoxy]phenyl- dihydrochloride 4-(2-pyridinyl) 2-pyrimidinamine339 Ex. 13 [3-[2-(4-morpho- N--[3-[2-(4-mor- 179- linyl)-ethoxy]- pholinyl)- 181 phenyl]guanidine ethoxy]phenyl]- monohydrochloride 4-(4-pyridinyl)- 2-pyrimidinamine340 Ex. 24 [3-[2-(4-morpho- N--[3-[2-(4-mor- 134- linyl)ethoxy]- pholinyl)ethoxy]- 136 phenyl]guanidine phenyl]-4-(2- monohydrochloride thienyl)-2-pyri- midinamine341 Ex. 10 [3-[2-(4-morpho- 4-(2-furanyl)-N-- 88- linyl)ethoxy]- [3-[2-(4-morpho- 90 phenyl]guanidine linyl)ethoxy]- monohydrochloride phenyl]-2-pyri- midinamine342 Ex. 24 1-[[4-[(Aminoi- 1-Methyl-4-[[4- 173- minomethyl)amino]- (2-thienyl)-2- 175 phenoxy]acetyl]-4- pyrimidinyl]- methyl piperazine phenoxy]acetyl)- dihydrochloride piperazine343 Ex. 24 (4-chlorophenyl) N--(4-chlorophe- 185- guanidine carbonate nyl)-4-(2-thienyl)-2- 186 pyrimidinamine344 Ex. 26 [2-[bis(1-methyl- N--[2-[2-[bis(1- 54- ethyl)amino[ethoxy methylethyl) 57 [guanidine hydro- amino]ethoxy] chloride phenyl]-4-(3-pyri- dinyl)-2-pyrimidi- namine______________________________________
The disease diabetes mellitus is characterized by metabolic defects in the production and utilization of glucose which results in the failure to maintain appropriate blood sugar levels. The result of this defect is elevated blood glucose or hyperglycemia. Research on the treatment of diabetes has centered on attempts to normalize fasting and postprandial blood glucose levels. Treatments have included parenteral administration of exogenous insulin, oral administration of drugs and dietary therapies.
Two major forms of diabetes mellitus are now recognized. Type I diabetes, or insulin-dependent diabetes, is a result of an absolute deficiency of insulin, the hormone which regulates glucose utilization. Type II diabetes, or insulin-independent diabetes, often occurs in the face of normal, or even elevated, levels of insulin and appears to be the result of the inability of tissues to respond appropriately to insulin.
The compounds of the present invention and the pharmacologically active acid-addition salts thereof, effectively lower blood glucose levels when administered orally to genetic strains of hyperglycemic mice which are animal models of type II diabetes. The exact mechanism by which they act is not known and the invention should not be construed as limited to any particular mechanism of action. As effective hypoglycemic agents, these compounds are useful for the treatment of hyperglycemia in type II diabetes.
The compounds of this invention were tested for hypoglycemic activity according to the following procedure.
Obese mice [C57 B1/6J (ob/ob)], their lean littermates (ob/+ or +/+) and diabetic mice [C57 B1/Ks (db/db)] and their non-diabetic littermates (db/+ or +/+) were obtained from Jackson Laboratories, Bar Harbor, Maine. Obese mice were 8 weeks of age and diabetic mice were 9 weeks of age at the start of the test.
The test compounds were dissolved in methanol, mixed with powdered Purina rodent chow on a weight of compound to weight of chow basis and thoroughly dried.
Groups of 4 control mice received vehicle (methanol) treated chow.
Groups of 4 test mice were fed ad libitum for one month and food consumption was measured daily (on week days) by weighing the food bins before and after the addition of fresh chow. Thus a 40 g mouse fed the test compound at a concentration of 0.02% of the diet would receive a dose of 20 mg/kg/day if it ate 4 g of chow per day.
Blood samples were collected before the first treatment and once at the end of each week of treatment by retro-orbital puncture using the end of each week of treatment by retro-orbital puncture using herparinized capillary tubes. Plasma was separated by centrifugation in a Beckman microfuge for 5 minutes. Plasma glucose concentrations were determined with the Beckman Glucose Analyzer which uses a glucose oxidase method.
The results of this test on representative compounds of this invention appear in Table X.
TABLE X__________________________________________________________________________Effect of Test Compounds on Blood Glucose Type Dose Blood Glucose Levels in mg/100 ml of % DaysCOMPOUND Mice (W/W) 0 5 7 14 21 28__________________________________________________________________________ --N--(4-methylphenyl)-4-(4- ob/ob 0.1 219 137pyridinyl)-2-pyrimidinamine ob/ob 0.1 210 118 80 ob/ob 0.025 209 223 166 --N--(4-Chlorophenyl)-4-(2- ob/ob 0.1 212 160thienyl)-2-pyrimidinanine ob/ob 0.025 220 148 134 --N--(4-ethylphenyl) -4-(4- ob/ob 0.1 216 181pyridinyl)-2-pyrimidinamine ob/ob 0.1 223 1644-(2-furanyl)- --N--phenyl-2- ob/ob 0.1 214 166pyrimidinamine --N--[4-(1,1-Dimethylethyl) ob/ob 0.1 208 114 175phenyl]-4-(3-pyridinyl)-2- ob/ob 0.1 214 169 155pyrimidinamine ob/ob 0.1 218 124 ob/ob 0.1 229 118 ob/ob 0.1 225 120 116 131 135 ob/ob 0.05 214 139 143 180 188 ob/ob 0.01 214 163 138 181 162 db/db 0.1 426 390 174 281 207 db/db 0.05 429 314 293 250 270 db/db 0.01 431 335 407 400 499 --N--[4-(Dimethylamino)phenyl]- ob/ob 0.1 240 1384-(4-pyridinyl)-2- ob/ob 0.1 230 147pyrimidinamine --N--[4-[3-(Dimethylamino)propoxy] ob/ob 0.1 215 234phenyl]-4-(3-pyridinyl)-2-pyrimidinamine --N--[4-[2-Diethylamino)ethoxy] ob/ob 0.1 220 191phenyl]-4-(3-pyridinyl)-2-pyrimidinamine --N'--[4-(2-Benzofurnayl)-2- ob/ob 0.1 229 153pyrimidinyl)- --N, --N--dimethyl- ob/ob 0.1 202 1471,4-benzenediamine ob/ob 0.1 223 144 --N--[4-[2-(Dimethylam- ob/ob 0.1 218 151 167ino)ethoxy]phenyl]-4- ob/ob 0.1 228 144(4-pyridinyl)-2- ob/ob 0.1 225 134pyrimidinamine ob/ob 0.1 232 148 128 155 140 ob/ob 0.05 230 158 198 196 163 ob/ob 0.01 236 163 252 175 177 db/db 0.1 369 410 403 328 222 db/db 0.05 400 277 404 329 250 db/db 0.01 368 393 321 494 336 --N--[4-(1 --H--Imidazol-1- db/db 0.1 424 397 233yl)phenyl]4-(4-pyri- ob/ob 0.1 219 128dinyl)-2-pyrimidin- ob/ob 0.025 210 200 148amine ob/ob 0.1 211 105 140 ob/ob 0.1 222 119 132 ob/ob 0.01 219 158 159 ob/ob 0.025 222 157 175 --N,N--Diethyl- --N.sup.1 --]4- ob/ob 0.1 223 138(3-pyridinyl)-2-pyrim- ob/ob 0.1 210 163idinyl]-1,4-benzene- ob/ob 0.1 216 153diamine --N--[4-(1H--Imidazol- ob/ob 0.1 225 1281-yl)phenyl]-4-(3- ob/ob 0.025 208 159 171pyridinyl)-2-pyrim- ob/ob 0.1 218 127 131idinamine --N--[4-(1 --H--Imidazol- ob/ob 0.1 217 1711-yl)phenyl]-4-(2- ob/ob 0.1 223 167pyridinyl)-2-pyrimid- ob/ob 0.1 234 141inamine4-(2-Furnayl)- --N--[4- ob/ob 0.1 227 137(1 --H--imidazol-1-yl) ob/ob 0.025 215 164 244phenyl]-2-pyrimidin- ob/ob 0.1 214 140 160amine --N--[4-(1 --H--Imidazol- ob/ob 0.1 221 109 1161-yl)phenyl]-4-(2- ob/ob 0.025 221 147 171thienyl)-2-pyrimid- ob/ob 0.01 217 212 161inamine ob/ob 0.1 224 125 ob/ob 0.1 203 131 ob/ob 0.1 231 126 ob/ob 0.1 218 134 ob/ob 0.025 218 175 185 ob/ob 0.1 220 135 117 db/db 0.1 423 492 3494-[[4-(3-Pyridinyl)- ob/ob 0.1 219 1222-pyrimidinyl]amino] ob/ob 0.1 240 147benzenesulfonamide ob/ob 0.1 216 185 ob/ob 0.1 229 142 ob/ob 0.1 228 211 --N--(3-chlorophenyl)-4- ob/ob 0.1 220 127(4-pyrindinyl-2- ob/ob 0.1 237 163pyrimidinamine ob/ob 0.1 216 135 ob/ob 1.0 205 157 ob/ob 0.025 210 157 135 ob/ob 0.1 212 173 129 --N--(3-Chlorophenyl)- ob/ob 0.1 205 1354-(3-pyridinyl)-2 ob/ob 0.025 221 205 131pyrimidinamine ob/ob 0.1 244 211 138 --N--[4-(4-Methyl-1- ob/ob 0.1 212 236piperazinyl)phenyl]-4-(3-pyridinyl)-2-pyrimidinamine --N--(3-chlorophenyl)- ob/ob 0.1 207 2044-(2-pyridinyl)-2-pyrimidinamine4-(2-Furnayl)- --N--[4- ob/ob 0.1 203 149(4-methyl-1-piper- ob/ob 0.025 210 179 130azinyl)phenyl]-2- ob/ob 0.1 229 163 141pyrimidinamine4-(2-Furanyl)- --N-- ob/ob 0.1 221 132(3-methoxyphenyl)- ob/ob 0.1 239 1132-pyrimidinamine ob/ob 0.1 217 162 ob/ob 0.1 219 209 --N--[4-(4-Methyl-1- ob/ob 0.1 203 188piperazinyl)phenyl]-4-(2-thienyl)-2-pyrimidinamine --N--[4-(4-Methyl- ob/ob 0.1 204 2101-piperazinyl)phenyl]-4-(2-pyridinyl)-2-pyrimidinamine --N--[4-(4-Methyl- ob/ob 0.1 204 118 124 178 1611-piperazinyl)phenyl]- ob/ob 0.025 210 157 200 152 2024-(4-pyridinyl)-2- ob/ob 0.01 210 130 192 178 147pyrimidinamine db/db 0.1 406 273 140 178 279 ob/ob 0.1 221 125 ob/ob 0.1 233 131 ob/ob 0.1 226 117 ob/ob 0.1 215 138 ob/ob 0.025 231 154 134 ob/ob 0.1 223 171 137 --N--[3-(1 --H--Imidazol- ob/ob 0.1 225 1731-yl)phenyl]-4-(3-pyridinyl)-2-pyrimidinamine --N--[4-[2-(Diethylamino) ob/ob 0.1 228 154ethoxy]phenyl]-4-(2- ob/ob 0.1 215 137thienyl)-2-pyrimidinamine --N--[2-[2-[Bis(1-methyl- ob/ob 0.1 228 153ethyl)amino]ethoxy]phenyl]-4-(3-pyridinyl)-2-pyrimid-inamine__________________________________________________________________________

Number	Name	Date
3980781	Snell et al.	Sep 1976
4263300	Stahle et al.	Apr 1981
4512993	Lesher et al.	Apr 1985

4,5,6-substituted-N-(substituted-phenyl)-2-pyrimidinamines

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