Patent Application
20240049716
This application claims priority to Singapore application number 10201902068U filed on 7 Mar. 2019, the disclosure of which is hereby incorporated by reference.
The present invention relates to a composite, a method of preparing the same and uses thereof.
Microbial infections and the development of antimicrobial resistance have received attention as one of the most critical issues facing the public health and security. The creation of clean antimicrobial surfaces with long-term stabilities and activities have tremendous applications involving almost all aspects of daily life, such as medical devices, hospital surfaces, textiles, packaging, electrical appliances, marine antifouling, filters and public surfaces.
Inorganic antimicrobial materials, especially semiconductor antimicrobial materials, are less prone to chemical contamination and possess long-term stability. Some metal or metal oxides, such as silver, zinc oxide and titanium oxide particles, have been used as antimicrobial ingredients in various products or in antimicrobial surface coatings. However, these materials have various limitations, such as heavy metal contamination/toxicity for silver-based materials and low antimicrobial efficacies due to dependence on photo irradiation and uncertain nano-toxicity for zinc oxide and titanium oxide materials. Redox active Zn/ZnO, Zn/ZnS and Zn/ZnSxO1-x composite materials have been developed and possess excellent antimicrobial property. However, these types of inorganic composites have difficulties in formulation and dispersion into various matrices.
Therefore, there is a need to provide a process and a composite material for antimicrobial activity that overcome or ameliorate one or more of the disadvantages mentioned above.
In one aspect, the present disclosure relates to a composite comprising an inorganic metal or alloy core at least partially covered by a protection layer comprising a carboxylate salt, a metal oxide, a metal oxide salt or a metal-amino acid complex.
Advantageously, the composite may demonstrate an antimicrobial activity in the form of antibacterial and antifungal effects. Thus, the composite may be used on its own as a general antimicrobial agent or antimicrobial surface coating or mixed in other systems as an antimicrobial additive.
Further advantageously, the composite may be highly active and stable and may exhibit long-term activity.
Still further advantageously, the composite may be easily synthesized, enabling scale-up manufacturing. The composite may also be readily formulated and dispersed into various matrices, thus it may be provided in different forms, such as films, plates and particles.
In another aspect, the present disclosure relates to a method of preparing the composite comprising an inorganic metal or alloy core at least partially covered by a protection layer comprising a carboxylate salt, a metal oxide, or a metal oxide salt or a metal-amino acid complex, comprising the step of mixing a metal or alloy with an acid, at elevated temperature for a time duration, optionally in the presence of a solvent.
Advantageously, the method of preparing the composite may be applied on various metal or alloy surfaces to generate activated surfaces and impose antimicrobial effect.
In another aspect, the present disclosure relates to use of a composite as an antimicrobial agent, wherein said composite comprises an inorganic metal or alloy core at least partially covered by a protection layer comprising a carboxylate salt, a metal oxide, a metal oxide salt or a metal-amino acid complex.
Advantageously, the use of the composite may cause a 5-log reduction of gram-positive bacteria, gram-negative bacteria or fungi population within 18 hours.
In another aspect, the present disclosure relates to a composite for use in the treatment or prophylaxis of microbial infection, wherein said composite comprises an inorganic metal or alloy core at least partially covered by a protection layer comprising a carboxylate salt, a metal oxide, a metal oxide salt or a metal-amino acid complex.
In another aspect, the present disclosure relates to use of a composite in the manufacture of a medicament for the treatment or prophylaxis of microbial infection, wherein said composite comprises an inorganic metal or alloy core at least partially covered by a protection layer comprising a carboxylate salt, a metal oxide, a metal oxide salt or a metal-amino acid complex.
In another aspect, the present disclosure relates to a method of inhibiting microbial activity or treating a microbial infection or disease comprising administering an antimicrobial composition comprising the composite as disclosed herein to a subject or applying the antimicrobial composition on a surface.
The following words and terms used herein shall have the meaning indicated:
The term “composite” as used herein refers to a material made of two or more constituent components that are of different physical and/or chemical properties such that when combined, the resulting material has characteristics different from the constituent components and the individual components remain separate and distinct within the finished structure.
The term “antimicrobial” as used herein refers to the capability to cause cell inhibition, cell injury, cell death or to control the growth of target bacteria and fungi microorganisms.
The term “additive” as used herein refers to a substance that is added to another substance or product in minor quantities to impart or improve certain desired performance properties.
Unless specified otherwise, the terms “comprising” and “comprise”, and grammatical variants thereof, are intended to represent “open” or “inclusive” language such that they include recited elements but also permit inclusion of additional, unrecited elements.
As used herein, the term “about”, in the context of concentrations of components of the formulations, typically means +/−5% of the stated value, more typically +/−4% of the stated value, more typically +/−3% of the stated value, more typically, +/−2% of the stated value, even more typically +/−1% of the stated value, and even more typically +/−0.5% of the stated value.
Throughout this disclosure, certain embodiments may be disclosed in a range format. It should be understood that the description in range format is merely for convenience and brevity and should not be construed as an inflexible limitation on the scope of the disclosed ranges. Accordingly, the description of a range should be considered to have specifically disclosed all the possible sub-ranges as well as individual numerical values within that range. For example, description of a range such as from 1 to 6 should be considered to have specifically disclosed sub-ranges such as from 1 to 3, from 1 to 4, from 1 to 5, from 2 to 4, from 2 to 6, from 3 to 6 etc., as well as individual numbers within that range, for example, 1, 2, 3, 4, 5, and 6. This applies regardless of the breadth of the range.
Certain embodiments may also be described broadly and generically herein. Each of the narrower species and sub-generic groupings falling within the generic disclosure also form part of the disclosure. This includes the generic description of the embodiments with a proviso or negative limitation removing any subject matter from the genus, regardless of whether or not the excised material is specifically recited herein.
Exemplary, non-limiting embodiments of a composite will now be disclosed.
The composite comprises an inorganic metal or alloy core at least partially covered by a protection layer comprising a carboxylate salt, a metal oxide, a metal oxide salt or a metal-amino acid complex.
The core may comprise a metal which may be selected from the group consisting of Group 2, Group 4, Group 7, Group 8, Group 12 or Group 13 of the Periodic Table of Elements, combinations thereof and alloys thereof.
The metal may be selected from the group consisting of zinc, iron, aluminium, titanium, magnesium, and manganese. The metal may be zinc, iron and titanium.
An acid may be used to form the protection layer. Depending on the type of acid used, this will determine the type of protection layer formed, whether the protection layer is a carboxylate salt, a metal oxide, a metal oxide salt or a metal-amino acid complex. The protection layer may also be a hydrated salt. The acid may be selected from the group consisting of organic acids, inorganic acids, and mixtures thereof. The metal (as defined above) or alloy may be reacted with the acid to form the protection layer. Where the acid is an organic acid, the protection layer is a carboxylate salt or an inorganic/organic complex. The carboxylate salt may be a metal carboxylate salt, where the metal of the metal carboxylate salt is as provided above. Where the acid is an inorganic acid, the protection layer is a metal oxide, metal oxide salt or hydrated metal oxide salt.
The organic acid may be selected from the group consisting of carboxylic acids, amino acids, and mixtures thereof.
The carboxylic acid may be a saturated monocarboxylic acid of the formula CH3(CH2)nCOOH, wherein n may be an integer from 0 to 20.
The carboxylic acid may be selected from the group consisting of saturated fatty acids, unsaturated fatty acids, aromatic carboxylic acids, dicarboxylic acids, tricarboxylic acids, keto acids, α-hydroxyl acids, and divinylether fatty acids.
The carboxylic acid may be selected from the group consisting of methanoic acid, ethanoic acid, propanoic acid, butanoic acid, 2-methylpropanoic acid, pentanoic acid, 3-methylbutanoic acid, hexanoic acid, heptanoic acid, octanoic acid, nonanoic acid, decanoic acid, undecanoic acid, dodecanoic acid, tridecanoic acid tetradecanoic acid, pentadecanoic acid, hexadecanoic acid, heptadecanoic acid, octadecanoic acid, nonadecanoic acid, eicosanoic acid, myristoleic acid, palmitoleic acid, sapienic acid, oleic acid, elaidic acid, vaccenic acid, linoleic acid, linoelaidic acid, α-linoleic acid, arachidonic acid, oxalic acid, succinic acid, adipic acid, maleic acid, fumaric acid, aldaric acid, citric acid, isocitric acid, pyruvic acid, acetoacetic acid, levulinic acid, benzoic acid, salicyclic acid, phthalic acid, glyceric acid, glycolic acid, lactic acid, tartaric acid, and colneleic acid.
The amino acid may be selected from the group consisting of proteinogenic amino acids, non-proteinogenic amino acids, and mixtures thereof.
The amino acid may be selected from the group consisting of arginine, histidine, lysine, phenylalanine, tryptophan, glutamic acid, aspartic acid, tyrosine, γ-aminobutyric acid, carnitine, levothyroxine, hydroxyproline, and selenomethionine. The amino acid may be histidine.
The inorganic acid may be selected from the group consisting of phosphoric acid, polyphosphoric acids, tungstic acids, vanadic acid, molybdic acid, and heteropolyacids.
The protection layer may be polymeric or non-polymeric.
The protection layer may be porous or non-porous.
The protection layer may be regarded as a shell layer.
The size of the inorganic core may be about 0.01 to about 100 μm, about 0.05 to about 100 μm, about 0.1 to about 100 μm, about 0.5 to about 100 μm, about 1 to about 100 μm, about 5 to about 100 μm, about 10 to about 100 μm, about 50 to about 100 μm, about 0.01 to about 0.05 μm, about 0.01 to about 0.1 μm, about 0.01 to about 0.5 μm, about 0.01 to about 1 μm, about 0.01 to about 5 μm, about 0.01 to about 10 μm, about 0.01 to about 50 μm, about 0.05 to about 0.1 μm, about 0.05 to about 0.5 μm, about 0.05 to about 1 μm, about 0.05 to about 5 μm, about 0.05 to about 10 μm, about 0.05 to about 50 μm, about 0.1 to about 0.5 μm, about 0.1 to about 1 μm, about 0.1 to about 5 μm, about 0.1 to about 10 μm, about 0.1 to about 50 μm, about 0.5 to about 1 μm, about 0.5 to about 5 μm, about 0.5 to about 10 μm, about 0.5 to about 50 μm about 1 to about 5 μm, about 1 to about 10 μm, about 1 to about 50 μm, about 5 to about 10 μm, about 5 to about 50 μm, or about 10 to about 50 μm.
The thickness of the protection layer may be about 5 to about 1000 nm, about 8 to about 1000 nm, about 10 to about 1000 nm, about 20 to about 1000 nm, about 50 to about 1000 nm, about 80 to about 1000 nm, about 100 to about 1000 nm, about 200 to about 1000 nm, about 500 to about 1000 nm, about 800 to about 1000 nm, about 5 to about 8 nm, about 5 to about 10 nm, about 5 to about 20 nm, about 5 to about 50 nm, about 5 to about 80 nm, about 5 to about 100 nm, about 5 to about 200 nm, about 5 to about 500 nm, about 5 to about 800 nm, about 8 to about 10 nm, about 8 to about 20 nm, about 8 to about 50 nm, about 8 to about 80 nm, about 8 to about 100 nm, about 8 to about 200 nm, about 8 to about 500 nm, about 8 to about 800 nm, about 10 to about 20 nm, about 10 to about 50 nm, about 10 to about 80 nm, about 10 to about 100 nm, about 10 to about 200 nm, about 10 to about 500 nm, about 10 to about 800 nm, about 20 to about 50 nm, about 20 to about 80 nm, about 20 to about 100 nm, about 20 to about 200 nm, about 20 to about 500 nm, about 20 to about 800 nm, about 50 to about 80 nm, about 50 to about 100 nm, about 50 to about 200 nm, about 50 to about 500 nm, about 50 to about 800 nm, about 80 to about 100 nm, about 80 to about 200 nm, about 80 to about 500 nm, about 80 to about 800 nm, about 100 to about 200 nm, about 100 to about 500 nm, about 100 to about 800 nm, about 200 to about 500 nm, about 200 to about 800 nm, or about 500 to about 800 nm.
The composite may have a protection layer content of about 0.1% to about 20% by weight, 0.5% to about 20% by weight, 0.8% to about 20% by weight, 1% to about 20% by weight, 5% to about 20% by weight, 8% to about 20% by weight, 10% to about 20% by weight, 15% to about 20% by weight, 18% to about 20% by weight, 0.1% to about 0.5% by weight, 0.1% to about 0.8% by weight, 0.1% to about 1% by weight, 0.1% to about 5% by weight, 0.1% to about 8% by weight, 0.1% to about 10% by weight, 0.1% to about 15% by weight, 0.1% to about 18% by weight, 0.5% to about 0.8% by weight, 0.5% to about 1% by weight, 0.5% to about 5% by weight, 0.5% to about 8% by weight, 0.5% to about 10% by weight, 0.5% to about 15% by weight, 0.5% to about 18% by weight, 0.8% to about 1% by weight, 0.8% to about 5% by weight, 0.8% to about 8% by weight, 0.8% to about 10% by weight 0.8% to about 15% by weight, 0.8% to about 18% by weight, 1% to about 5% by weight, 1% to about 8% by weight, 1% to about 10% by weight, 1% to about 15% by weight, 1% to about 18% by weight, 5% to about 8% by weight, 5% to about 10% by weight, 5% to about 15% by weight, 5% to about 18% by weight, 8% to about 10% by weight, 8% to about 15% by weight, 8% to about 18% by weight, 10% to about 15% by weight, 10% to about 18% by weight, or 15% to about 18% by weight, based on the weight of the inorganic core.
The particle size of the composite may be in the range of about 100 nm to 500,000 nm.
The composite may be in different forms, such as films, plates and particles.
Exemplary, non-limiting embodiments of a method of preparing the composite will now be disclosed.
The method of preparing the composite comprising an inorganic metal or alloy core at least partially covered by a protection layer comprising a carboxylate salt, a metal oxide, a metal oxide salt or a metal-amino acid complex, may comprise the step of mixing a metal or alloy with an acid, at elevated temperature for a time duration, optionally in the presence of a solvent.
The method may further comprise the steps of cooling the mixture to room temperature, collecting the composite by filtration and repeated washing of the composite with a suitable solvent.
The solvent may be selected from the group consisting of dimethylformamide (DMF), dimethyl sulfoxide (DMSO), dimethylacetamide (DMAc), N-methyl-2-pyrrolidone (NMP), acetonitrile, acetone, ethanol and mixtures thereof.
The elevated temperature used in the method may be of about 50° C. to about 180° C., about 70° C. to about 180° C., about 90° C. to about 180° C., about 110° C. to about 180° C., about 130° C. to about 180° C., about 150° C. to about 180° C., about 170° C. to about 180° C., about 50° C. to about 70° C., about 50° C. to about 90° C., about 50° C. to about 110° C., about 50° C. to about 130° C., about 50° C. to about 150° C., about 50° C. to about 170° C., about 70° C. to about 90° C., about 70° C. to about 110° C., about 70° C. to about 130° C., about 70° C. to about 150° C., about 70° C. to about 170° C., about 90° C. to about 110° C., about 90° C. to about 130° C., about 90° C. to about 150° C., about 90° C. to about 170° C., about 110° C. to about 130° C., about 110° C. to about 150° C., about 110° C. to about 170° C., about 130° C. to about 150° C., about 130° C. to about 170° C., or about 150° C. to about 170° C.
The time duration used in the method may be of about 1 to about 50 hours, about 5 to about 50 hours, about 10 to about 50 hours, about 20 to about 50 hours, about 30 to about 50 hours, about 40 to about 50 hours, about 45 to about 50 hours, about 1 to about 5 hours, about 1 to about 10 hours, about 1 to about 20 hours, about 1 to about 30 hours, about 1 to about 40 hours, about 1 to about 45 hours, about 5 to about 10 hours, about 5 to about 20 hours, about 5 to about 30 hours, about 5 to about 40 hours, about 5 to about 45 hours, about 10 to about 20 hours, about 10 to about 30 hours, about 10 to about 40 hours, about 10 to about 45 hours, about 20 to about 30 hours, about 20 to about 40 hours, about 20 to about 45 hours, about 30 to about 40 hours, about 30 to about 45 hours, or about 40 to about 45 hours.
The composite prepared from this method is as described above.
Exemplary, non-limiting embodiments of uses of a composite will now be disclosed.
The composite is as described above. The composite may be used as an additive in a composition or a formulation.
When the composite is used as an additive in a composition or a formulation, the composition or formulation may be non-therapeutic.
The composition or formulation may be selected from the group consisting of antimicrobial plastics, antimicrobial pigments, and antimicrobial textiles.
The concentration of the composite may be in the following ranges:
There is also provided use of a composite as an antimicrobial agent wherein said composite comprises an inorganic metal or alloy core at least partially covered by a protection layer comprising a carboxylate salt, a metal oxide, a metal oxide salt or a metal-amino acid complex.
The composite is as described above.
The microorganisms may be selected from the group consisting of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilius, Klebsiella pneumonia, Cryptococcus neoformans and Candida albicans.
The use of the composite may cause a 5-log reduction of gram-positive bacteria, gram-negative bacteria or fungi population within 18 hours.
There is also provided a composite for use in the treatment or prophylaxis of microbial infection, wherein said composite comprises an inorganic metal or alloy core at least partially covered by a protection layer comprising a carboxylate salt, a metal oxide, a metal oxide salt or a metal-amino acid complex.
There is also provided use of a composite, in the manufacture of a medicament for the treatment or prophylaxis of microbial infection, wherein said composite comprises an inorganic metal or alloy core at least partially covered by a protection layer comprising a carboxylate salt, a metal oxide, a metal oxide salt or a metal-amino acid complex.
The composite is as described above.
The microbial infection may be caused by a microbe selected from the group consisting of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilius, Klebsiella pneumonia, Cryptococcus neoformans and Candida albicans.
Exemplary, non-limiting embodiments of a method of inhibiting microbial activity or treating a microbial infection or disease will now be disclosed.
There is also provided a method of inhibiting microbial activity or treating a microbial infection or disease comprising administering an antimicrobial composition comprising the composite as disclosed herein to a subject or applying the antimicrobial composition on a surface.
The accompanying drawings illustrate a disclosed embodiment and serves to explain the principles of the disclosed embodiment. It is to be understood, however, that the drawings are designed for purposes of illustration only, and not as a definition of the limits of the invention.
Non-limiting examples of the invention will be further described in greater detail by reference to specific Examples, which should not be construed as in any way limiting the scope of the invention.
All the reagents were obtained from commercial suppliers and used without further purification. Commercially available zinc powder of 0.4 μm to 10 μm particle size, and titanium powder of 1 μm to 10 μm were purchased from Sigma-Aldrich (of St Louis, Missouri of the United States of America).
The various synthesized composites were subjected to imaging using the scanning electron microscope-energy dispersive X-ray (SEM-EDX) (model JEOL JSM-7400E) at an accelerating voltage of 5 kV. Prior to SEM imaging, the composites were sputter-coated with thin platinum film using the high resolution sputter coater (model JEOL JFC-1600 Auto Fine Coater). The surfaces of the composites were characterized using the transmission electron microscopy (TEM) (model FEI Tecnai F30), field emission transmission electron microscopy-energy dispersive X-ray (FTEM-EDX) (model FEI Tecnai F30), and Fourier-transform infrared spectroscopy (FTIR) (model PerkinElmer Spectrum 100).
Microorganisms (gram-negative bacteria E. coli ATCC 8739, gram-positive bacteria S. aureus ATCC 6538P and Candida albicans ATCC-10231) used in the antimicrobial characterization of the synthesized composites were purchased from American Type Culture Collection (ATCC) and re-cultured from lyophilised powders according to suggested protocols. The enrichment medium used for bacteria was a general purpose medium, tryptic soy broth (TSB), purchased from Beckton Dickinson Diagnostics (Singapore). The enrichment medium used for fungi was yeast and malt extract broth (YMB), purchased from Beckton Dickinson Diagnostics (Singapore). TSB and YMB were prepared according to manufacturer's instructions. Prior to microbial experiments, microbial cultures were refreshed on nutrient agar (Millipore, Singapore) plates from stock. Fresh microbial suspensions were grown overnight at 37° C. in 5 ml of TSB or YMB. Microbial cells were collected at the logarithmic stage of growth and the suspensions were adjusted to optical density (OD600) of 0.07. Prior to antimicrobial tests, the suspensions were further diluted by 100 times.
Fresh zinc powder (2 g) was mixed with benzoic acid (0.2 g) in N, N-dimethylformamide (20 ml), and the mixture was stirred at 100° C. for 20 hours. After cooling to room temperature, solid residuals of the composite A were collected by filtration and washed with acetone. The particle size range of the synthesized composite A was measured to be about 400 nm to 1,000 nm.
A schematic representation of the structure of the composite formed is depicted in
The presence of a protection layer, which was effected by the precursor benzoic acid, was confirmed by
Fresh zinc powder (2 g) was mixed with histidine (0.1 g) in ethanol (0.2 ml), and the mixture was stirred at 120° C. for 24 hours. After cooling to room temperature, solid residuals of the composite B were collected. The particle size range of the synthesized composite B was measured to be about 400 nm to 1,000 nm.
A schematic representation of the structure of the composite formed is depicted in
Fresh titanium powder (1 g) was mixed with concentrated phosphoric acid (0.03 ml) and the mixture was stirred at 130° C. for 6 hours. After cooling to room temperature, solid residuals of the composite C were collected. The particle size range of the synthesized composite C was measured to be about 400 nm to 1,000 nm.
A schematic representation of the structure of the composite formed is depicted in
To test the antimicrobial properties of the synthesized composites, 20 mg synthesized composite was dispersed in ethanol and coated onto a glass slide with a dimension of 2.5 cm×2.5 cm. An untreated (blank) glass slide was used as a negative control. The antimicrobial properties of the surfaces were evaluated by the JIS Z 2801/ISO 22196 method against E. coli (gram-negative, ATCC 8739), S. aureus (gram-positive, ATCC 6538P) and C. albicans (fungi). Briefly, 20 mg of synthesized composite was dispersed on a pre-cleaned glass slide and an aliquot of diluted cell suspension (150 μL) (gram positive or gram negative bacteria at concentration of 106 CFU mL−1 or fungi at concentration of 105 CFU mL−1) was used to cover the surface of the glass slide completely. The glass slide was incubated at 37° C. for 18 hours, and the resultant surface was washed and diluted using TSB for bacteria and YMB for fungi, before spreading the washings on two nutrient agar plates. The nutrient agar plates were incubated overnight at 37° C. The resultant colonies grown on the nutrient agar plates were counted using standard plate counting techniques.
The number of colony forming units (cfu) per mL was calculated and compared against the negative control, to determine the log reduction and the effective killing efficiency of the composites. The number of colony forming units was assumed to be equivalent to the number of viable cells in suspension. The experiment was conducted in triplicates and average results were obtained. After the 18 hours incubation period, microbial growth was observed for the untreated glass slide such that there was an increase of 102 log units from 106 to 108 CFU mL−1, while for glass slides treated with composites A, B and C, there was no observable microbial growth for all three microorganisms tested. Based on log reduction data (Table 1), surfaces coated with composites A, B and C, all showed excellent antimicrobial properties. All tested microbes that were exposed to surfaces with the composites were killed after an 18 hours incubation period such that there were more than 5-log reductions of microbe population observed for E. coli, S. aureus and C. albicans, exhibiting the excellent antimicrobial properties of the synthesized composites.
To test the efficacy of the synthesized composites as additives in imparting antimicrobial properties to substrate materials such as plastics and pigments, polyethylene (PE) plastic and polyacrylic (PA) paint were doped with composite A at 2% and 1% by weight of the substrates respectively. Briefly, 10 g PE plastic was melted at about 200° C. and 2% (w/w) composite A was mixed with molten PE plastic and the resultant mixture was cast into a plate. For polyacrylic paint, commercially available PA paint (without antimicrobial agent) was mixed with 1% (w/w) composite A to create antimicrobial PA paint. The resultant paint was painted on glass surface and the painted surface was air-dried. Both the PE plastic and painted glass surface which are doped with composite A were evaluated for antimicrobial properties using the JIS Z 2801/ISO 22196 method as described previously.
To test the efficacy of the synthesized composites as additives in antimicrobial textiles, commercially available liquid detergent was mixed with acid-coated composite A at 1 g of composite A for every 150 ml of liquid detergent. The doped detergent liquid was added to textile and washed using the following procedures: 0.01 g composite A was mixed with 1.5 ml commercial liquid detergent and poured into 150 ml water to create a doped washing mixture. The textile (10 g) was added to the doped washing mixture and stirred for 1 hour at a temperature between 25° C. to 60° C. to simulate a washing cycle. After washing, the textile was rinsed with 150 ml of water twice. Thereafter the composite A doped textile was evaluated for antimicrobial properties using the JIS Z 2801/ISO 22196 method as described previously.
Based on log reduction data (Table 1), polyethylene plastic doped with composite A showed excellent antimicrobial properties where more than 5-log reductions of microbial population were observed for E. coli, S. aureus and C. albicans. Polyacrylic paint doped with composite A showed better killing efficacy for E. coli (>5-log reductions of population) than C. albicans (>2-log reductions of population). Textile doped with composite A showed excellent antimicrobial property where more than 5-log reductions of microbial population were observed for E. coli. The results showed that the synthesized composites can be mixed into other systems as additives to impart antimicrobial properties.
E. coli
S. aureus
C. albicans
The composite may be used as a general antimicrobial agent in various applications, such as but not limited to, consumer care, cosmetics, healthcare, personal care, public hygiene, general surface disinfection, antifouling, anti-molding, hospital and medical device disinfection, and agriculture industry.
It will be apparent that various other modifications and adaptations of the invention will be apparent to the person skilled in the art after reading the foregoing disclosure without departing from the spirit and scope of the invention and it is intended that all such modifications and adaptations come within the scope of the appended claims.
| Number | Date | Country | Kind |
|---|---|---|---|
| 10201902068U | Mar 2019 | SG | national |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/SG2020/050025 | 1/21/2020 | WO |
