Patent Application
20210003599
An impression left by the friction ridges of human skin, such as the skin of a human finger, contains information regarding the identity of the human. It is widely known that the appearance of the impression of the human finger, known as a fingerprint, is unique to each human and may be used to confirm the identity of the human. The appearance of the impression of the skin of other human body parts may also be unique to each human and so may also be used to confirm the identity of the human. Impressions of human skin, including but not limited to the skin of the human finger, may be called skin-prints.
In the present disclosure, it should be noted that the term skin-print is used to refer to the residue of a deposited skin-print rather than to the constituents of the skin-print residue when present on the human skin. The skin-print contains sweat which may include both eccrine sweat and sebaceous sweat.
In addition to the appearance of the impression left by human skin, the impression may contain chemical species which themselves may be detected in order to obtain further information.
For example, when a human intakes a substance (e.g. by ingestion, inhalation, injection or any other means) the substance may be metabolised by the human body giving rise to secondary substances known as metabolites. The presence of a particular metabolite can be indicative of a specific intake substance. The intake substance and/or metabolites may be present in sweat and, as such, may be left behind in a skin-print, e.g. a latent/residual fingerprint. Detection of such metabolites in a skin-print can be used as a non-invasive method of testing for recent lifestyle activity such as (but not limited to) drug use, or compliance with a pharmaceutical or therapeutic treatment regime. Other applications include drug rehabilitation, criminal justice and research into latent fingerprints.
Importantly, the taking of a skin-print is much simpler than obtaining other body fluids such as blood, saliva and urine, and is more feasible in a wider range of situations. Not only this but since the appearance of the skin-print itself provides confirmation of the identity of the person providing the skin-print, there can be greater certainty that the substance or substances in the skin-print are associated with the individual. This is because substitution of a skin-print, particularly a fingerprint, is immediately identifiable from appearance whereas substitution of, for example, urine, is not immediately identifiable from appearance. As such, testing for one or more substances in a skin-print provides a direct link between the one or more substances and the identity of the human providing the skin-print.
The applicant has demonstrated various techniques for chemical analysis of skin-prints, including the use of mass spectrometry, for example paper spray mass spectrometry. The applicant has also developed a lateral flow skin-print analysis technique as described in WO 2016/012812, published 28 Jan. 2016.
Obtaining an indication of a quantity, for example mass (or possibly volume), of a metabolite present in a skin-print sample may be more informative if given as a measure relative to quantity, for example by volume (or possibly by mass), of skin-print. This may be particularly applicable in situations where an acceptable threshold (measured in, for example, mass of analyte per unit volume of skin-print) is defined, for example by an independent standards agency.
For example, a relatively small amount of metabolite present in a relatively large volume/mass of skin-print may be less significant than a relatively larger amount of metabolite present in only a relatively small volume/mass of skin-print.
Accordingly, a need exists for a technique to determine a quantity of skin-print deposited in order to be able to determine an amount of analyte per unit of deposited skin-print.
Against this background, there is provided a method for analysing a skin-print, the method comprising:
Advantageously, therefore, the chemical test result may be contextualised relative to the quantity score. The quantity score may be or may be related to an indication of the mass of the skin-print or an indication of the volume of the skin-print or some other indication of the amount of skin-print present on the substrate.
Preferably, the quantity score provides a volume of the skin-print sample and the chemical analysis provides a mass of one or more analytes under test. Thus, the method may provide a result in terms of unit mass of analyte per unit volume of skin-print.
The present disclosure relates to a method of analysing a skin-print deposited on a substrate. A high level representation of the method is shown in the flow chart of
The method 100 involves receiving a skin-print on a substrate 110, obtaining a quantity score for the skin-print 120 and obtaining results of a chemical analysis of the skin-print 130 so as to allow the chemical test result to be contextualised relative to the quantity of skin-print.
The disclosure envisages a wide range of chemical analysis techniques and a wide range of quantity analysis techniques. Accordingly, the specific embodiments of chemical analysis technique and quantity analysis technique discussed herein should be interpreted as examples of a range of available options that fall within the scope of the claimed invention.
The term “quantity test” encompasses any technique that provides an objective measure that is, or is related to, the quantity of skin-print present on the substrate under test. One objective quantity test of a skin-print may involve determining mass of the skin-print. Another objective quantity test of a skin-print may involve determining volume of the skin-print.
It will be appreciated that the quantity of material deposited in a skin-print sample is likely to be modest. Accordingly, the quantity may not be straightforwardly measured. To measure mass or volume of skin-print directly and in a non-destructive manner may require expensive scientific instrumentation. This may not be possible in the field. For example, high precision balances that might be used to measure the mass of a substrate both before and after a skin-print has been deposited may be both expensive and require controlled environments such as anti-vibration tables and cleanroom conditions to avoid effects of vibrational noise and contamination. In order to obviate the need for such equipment, the applicant has developed techniques for determining a quantity score for a skin-print that rely upon determining mass/volume (to within a known level of uncertainty) by directly measuring other attributes of the skin-print.
Some of these techniques involve performing an optical analysis on the skin-print in order to determine the degree to which the optical properties of a substrate are altered by virtue of a skin-print deposited upon the substrate. The measurement of such optical properties has been found by the Applicant to relate to the measure of mass or volume of skin-print.
In an embodiment wherein the quantity score of a skin-print is related to the mass of the skin-print, the quantity score may simply be the mass of the skin-print. Or it may be proportional to the mass of the skin-print. Or it may be a function of the mass of the skin-print. Any approach by which the mass of the skin-print may be measured or derived may be appropriate.
Similarly, in an embodiment wherein the quantity score of a skin-print is related to the volume of the skin-print, the quantity score may simply be the volume of the skin-print. Or it may be proportional to the volume of the skin-print. Or it may be a function of the volume of the skin-print. Any approach by which the volume of the skin-print may be measured or derived may be appropriate.
It should also be understood that other characteristics of the skin-print (instead of or in addition to mass/volume) may contribute to the objective assessment of skin-print quantity.
The specific examples of quantity analysis and chemical analysis disclosed herein should be understood as merely exemplary rather than prescriptive.
Non-Exhaustive List of Techniques for Obtaining a Quantity Score
A quantity score may be obtained using a variety of different techniques, including the following.
Interferometry may be used to establish the skin-print's topography in order to determine skin-print volume. The interferometry technique may be or comprise white light interferometry.
Detection of the influence of a skin print on the passage or propagation of electromagnetic radiation may be used to determine mass or volume, perhaps by calibration with another technique such as white light interferometry.
Surface plasmon resonance imaging may be used, where the substrate material allows, to establish a topographical map of the skin-print from which volume can be determined.
In another example, obtaining a quantity score may involve emitting electromagnetic radiation towards the substrate and analysing how the electromagnetic radiation is influenced by the presence of a skin-print and the amount of skin-print deposited. A reflection of the electromagnetic radiation from the substrate may then be captured, recorded and analysed. The electromagnetic radiation may fall within the visible spectrum (either broadband spectrum or a specific wavelength) and the technique may involve determining the effect of the skin-print on the visible spectrum radiation.
In a more specific example, discussed in more detail below, a technique involves use of an optical waveguide having a fingerprint receiving region. A skin-print deposited on the fingerprint receiving region acts as a coupling device that selectively permits coupling of the optical radiation into/out of the waveguide dependent upon the amount of skin-print deposited.
In an alternative optical approach, an optical image may be taken of a deposited skin-print which is analysed using image processing software in order to provide an objective measure of the amount of skin-print deposited.
In a further alternative optical approach, an optical image may be taken of light reflected off a deposited skin-print and analysed using image processing software in order to provide an objective measure of the amount of skin-print deposited.
Still further examples of techniques that may be adopted as part of a quality scoring process include (but are not limited to) the following:
It is possible to implement many of these techniques on the substrate both before and after deposition of the skin-print in order to provide a reference or control with which a comparison may be made.
It is also envisaged that calibration of one or more quantity score techniques may be appropriate and/or indeed necessary. For example, in one scenario, an optical based quantity score technique may be calibrated using a technique based on white light interferometry. White light interferometry itself may provide a more direct route to quantity but may require a longer time and more complex apparatus. By contrast, the optical based techniques may be quicker and require less complicated apparatus but may provide a less direct route to quantity. Calibrating the optical based technique with white light interferometry may provide for increased confidence in quantity scores provided by the optical based technique and may be more amenable to calibration themselves using metrology standards.
Depending upon the specific combination of quantity analysis technique and chemical analysis technique, it may be that the order in which the techniques are adopted is important. This may particularly be the case if one technique might prejudice the outcome of the other. However, where there is no such potential prejudice, it may be that the quantity analysis and the chemical analysis are carried out in either order.
Non-Exhaustive List of Chemical Analysis Techniques
In the same way that a range of quantity analysis techniques falls within the scope of the disclosure, so also a range of chemical analysis techniques falls within the scope of the disclosure.
One possibility is to use mass spectrometry techniques including:
Raman Spectroscopy is still a further alternative chemical analysis technique.
Further alternatives include chromatography techniques including thin layer chromatography.
Further possibilities for chemical analysis techniques include skin-print development techniques including those employing substances such as Ninhydrin. This may be in combination with a separation method (e.g. thin film chromatography) whereby the chemical components are separated and then stained in order to aid in visualisation.
Further options include lateral flow analysis techniques such as those disclosed in the Applicant's previous patent publications including in application number PCT/GB2015/052157 published as WO 2016/012812.
In short, the disclosure envisages any appropriate chemical analysis technique from which one or more chemical constituents may be detected together with an indication of quantity of the chemical constituent.
Whatever chemical analysis technique is adopted, it may be that the chemical test result provided by it is in the form of a numerical output. In one example, the numerical output may be in the form of a precise mass of analyte in question. In another example, the numerical output may be a numerical indication that the mass of analyte falls within a particular mass range. In either of these examples, or in other cases, in order to contextualise the chemical test result, the result may be compared to the quantity score in order to provide a contextualised result.
The contextualised result may then be used to inform the intended assessment which may, for example, include whether or not the skin-print provides evidence of use of a particular drug or, for example, whether or not the skin-print provides evidence of complicity with a drug dosage regime.
The contextualised result may be compared to an independently determined threshold. One example would be an independently specified threshold at which drug use is considered clearly demonstrated. This might be measured in unit mass of drug metabolite per unit volume of skin-print.
Where the quantity score is determined by a technique other than direct measurement of the mass/volume of the skin-print, it may be necessary to calibrate the quantity score with a particular mass/volume or other metric.
In some circumstances it may be that a quantity analysis is performed at a different time and location from a chemical analysis technique. For example, it may be that the quantity analysis technique (perhaps a preliminary quantity analysis technique that precedes a secondary quantity analysis technique) is performed using portable apparatus that is available in the field and that the chemical analysis technique involves apparatus that is perhaps not portable or perhaps not available in the field. In this scenario, as in many others, it may be important that the quantity score obtained in the field using the quantity analysis technique is connected to the sample in question such that the result of the chemical analysis score is reconciled with the exact same sample to which the quality score relates. Accordingly, it may be that the sample substrate or an ancillary to the sample substrate has a unique identifier. The unique identifier could use any technique by which the sample substrate could be uniquely identified. It may comprise some form of tamper evident features in order to provide evidence of an attempt to disguise the provenance of the sample.
In any event, even where quantity analysis technique and chemical analysis technique are carried out one immediately after the other (or even simultaneously), being confident that the results of each technique on a particular sample are linked to one another may be particularly important. Thus, use of a unique identifier to which the results are linked has a very broad range of applications.
While the unique identifier might involve one or more of any number of unique identifier features known to the skilled person, examples of such identifiers may include an RFID tag, a QR code, a bar code (perhaps engraved into the substrate) or other tags, whether electronic, mechanical, optical biological or any other suitable means for providing unique identification.
The unique identifier may in some examples also possess properties that allow it to receive and store information on the provenance of the sample or its quantity score or its identity, for example where a suitable identification algorithm is used in the analysis instrument to determine the identity of the sample donor. This might comprise a fingerprint matching algorithm.
Detailed Example of an Optical Analysis Technique for Determining a Quantity Score
The Applicant has developed an optical analysis method and apparatus that may be used for determining a quantity score in accordance with the present disclosure.
The fingerprint receiving region 20 may provide a fixed area onto which a skinprint may be applied in order to increase consistency of area of skinprints between donors. The fixed area may be smaller than the average skinprint area. This may also have advantages for consistency if the same subject provides multiple prints, perhaps for different purposes.
The apparatus 200 comprises primary and secondary electromagnetic radiation sources 40, 80; a translucent waveguide 10 having a first end 12 and a second end 14; and first and second photodiodes 50, 60 configured to output a photodiode signal indicative of electromagnetic radiation detected by the first and second photodiodes 50, 60. The first photodiode 50 is configured to detect electromagnetic radiation emitted by the primary electromagnetic radiation source 40. The second photodiode 60 is configured to detect electromagnetic radiation emitted by the secondary electromagnetic radiation source 80.
The first end 12 of the translucent waveguide 10 comprises a fingerprint receiving region 20 on a first (upper) surface 16 of the translucent waveguide 10. The fingerprint receiving region 20 may be identified on the first surface 16 by virtue of one or more visible indications on or surrounding the fingerprint receiving region 20. Alternatively, the fingerprint receiving region 20 may be identified by a window bounded by a frame that obscures parts of the first surface 16 that do not form part of the fingerprint receiving region 20. The fingerprint receiving region 20 may be identified by other means.
In the illustrated embodiment, the primary electromagnetic radiation source 40 is configured to emit light towards a surface of the translucent waveguide 10 opposite the fingerprint receiving region 20. Depending on the angle of incidence of the primary electromagnetic radiation relative to the translucent waveguide 10, it may be that the first end 12 of the translucent waveguide 10 also comprises a grating coupler 17 for coupling the primary electromagnetic radiation into the translucent waveguide 10.
A surface of the translucent waveguide 10 in the vicinity of the fingerprint receiving region 20 may serve as a waveguide interface 18 through which electromagnetic radiation may be transmitted or in which electromagnetic radiation may be reflected, dependent upon circumstances. The waveguide interface 18 may or may not be different in surface properties when compared to a surface of the translucent waveguide 10 that surrounds the waveguide interface 18.
The first electromagnetic radiation source 40 is located towards the first end 12 of the translucent waveguide 10 and positioned so as to emit electromagnetic radiation towards the fingerprint receiving region 20 in such a way that the presence or absence of a skin-print in the fingerprint receiving region 20 influences transmission/reflection of the electromagnetic radiation at the waveguide interface 18. The angle of the first electromagnetic radiation source 40 relative to the plane of the waveguide interface 18 may be such that electromagnetic radiation that is coupled into the translucent waveguide 10 propagates along the translucent waveguide 10 in a direction towards the second end 14 of the translucent waveguide 10 and propagates therein by total internal reflection.
The second electromagnetic radiation source 80 is optically coupled to the translucent waveguide 10 towards the second end 14 such that electromagnetic radiation 75 emitted by the second electromagnetic radiation source 80 enters into the translucent waveguide 10 at an angle such that the electromagnetic radiation 75 passes directly through the optical waveguide regardless of whether a skin-print is present. In the illustrated embodiment of
The first photodiode 50 is located so as to detect electromagnetic radiation that is transmitted out of the second end 14 of the translucent waveguide 10. The second photodiode 60 is located so as to detect electromagnetic radiation that is transmitted across the second end 14 of the translucent waveguide 10, as shown in
While in
In the case that no skin-print is present on the skin-print receiving region 20, as is evident from
By contrast, as can be seen from
In very general terms, the greater the (influence of) the skin-print, the greater the amount of primary radiation detected at the first photodetector 50. Where no skin-print is present, little or no electromagnetic radiation 70 from the first electromagnetic radiation source 40 will arrive at and be detected by the photodiode 50. Where a well-defined, strong skin-print is present, a significant proportion of the electromagnetic radiation 70 from the first electromagnetic radiation source 40 will be coupled into the waveguide interface and will arrive at and be detected by the photodiode 50.
By having two electromagnetic radiation sources 40, 80 and two photodiodes 50, 60 wherein only one radiation source is affected by the presence or absence of a skinprint, the amount of secondary radiation detected at the second photodiode 60 can be used as a reference with which the amount of primary radiation detected at the first photodiode 50 can be compared. This allows for elimination of noise within the substrate.
This calculation provides a numeric assessment of the quantity and/or extent of skin-print 30 on the skin-print receiving region 20.
It may be that the first electromagnetic radiation source 40 is configured to emit electromagnetic radiation having a first wavelength or range of wavelengths and that the second electromagnetic radiation source 80 is configured to emit electromagnetic radiation having a second wavelength or range of wavelengths that may or may not be different to the first wavelength or range of wavelengths.
An example of the data processing that may be conducted using the apparatus of the embodiment of
It is the fact that the electromagnetic coupling through the waveguide interface 18 is influenced by the quantity of skin-print present that allows for an objective measure of the quantity of a skin-print to be provided by the technique. In brief, the greater the quantity of the skin-print, the greater is the coupling of the electromagnetic radiation 70 from the first electromagnetic radiation source 40. Indeed, it has been established by the Applicant that the amount of primary electromagnetic radiation detected at the first photodiode 50 correlates closely with the volume of the skinprint. The accuracy of this correlation increases when accounting for noise and losses in the substrate which are provided by the secondary electromagnetic radiation detected at the second photodiode 60 which is independent of the volume (or indeed the presence or absence of a skinprint).
It should be noted that
In
The apparatus shown in
Calibration of Optical Analysis Results Using Other Techniques
In order to confirm an objective relationship between the quantity scores obtained by indirect techniques for obtaining mass and/or volume of skin-print (such as the optical technique described above) and the actual quantity of the skin-print sample, the Applicant has calibrated its quantity scores using other techniques for determining skin-print quantity and hence skin-print quantity scores.
In one exemplary approach, white light interferometry has been used to obtain a surface profile of a skin-print. White light interferometry is a known technique for providing nanometre-accurate three-dimensional surface profile maps of substrates in the nanometre to centimetre surface area range. By obtaining a 3D surface map of the substrate, the volume of the deposited skin-print can be calculated by performing a three-dimensional integration of the volume beneath the surface profile of the deposited skin-print in order to obtain a volume of deposited skin-print, typically in nanolitres.
In this way, the Applicant has identified that a skin-print quantity score obtained using the optical analysis technique detailed above (and using the apparatus shown in
In particular, the Applicant has identified over a wide population a linear correlation between the extent to which electromagnetic radiation is coupled by an untreated latent residual skin-print into an optical waveguide and the volume of the said skin-print.
The correlation is such that the data provided by the optical analysis technique provide a value for the volume of skin-print present, within a small and defined level of uncertainty.
Potential Applications of the Method
The method of the present disclosure is applicable to a wide range of applications and scenarios. A number of exemplary applications are discussed further below.
One potential application for the method of the present disclosure may be simply to provide evidence that a sufficient volume of skin-print sample is provided in order for a reliable chemical analysis to be conducted. In this way, where an insufficient volume of skin-print has been provided (e.g. where the provider of a sample has sought to evade a test), it may be possible to reject the skin-print sample for chemical analysis in order (a) to avoid cost and (b) instead require the provider of the skin-print sample to provide another sample.
Another potential application for the method of the present disclosure may be to use the quantity test score together with the chemical test result to calculate an amount of a particular analyte of interest per unit of sample. For example, a result may be given in terms of volume of analyte per unit volume of sample, or mass of analyte per unit mass of sample, or mass of analyte per unit volume of sample, or volume of analyte per unit mass of sample, thereby providing a quantitative output.
In this way, it is possible to provide a measure using the same units as are commonly used in the analysis of oral fluid, urine or blood. As the skilled person will appreciate, the unit analyte per unit sample values will not be the same for any analyte. Indeed, for a particular individual, giving simultaneous samples of oral fluid, blood, urine and skin-print, the ratio of analyte to sample may be very different. While these measures are not fully interchangeable, they may be related.
The method may be applied in the context of providing confirmation that an individual is complying with a pharmaceutical or therapeutic treatment regime. In particular, the technique may be used to send data to for example a physician or a prescribing authority which might use the data to satisfy itself that the pharmaceutical or therapeutic treatment regime was being complied with prior to prescribing a future dose of the drug in question.
In a variation on the application of the method in a pharmaceutical or therapeutic treatment regime, the method may have applications in drug trials. Participants in a drug trial may be required to provide skin-print samples at a frequency of up to several times a day without the inconvenience that might result from the need to provide blood, urine or oral fluid samples. The skin-prints may be analysed immediately in the field and the data obtained thereby may be transmitted to the entity running the drug trail in real time in order to obtain granular data regarding the effect of the drug under test on the analytes in the skin-print.
A wide variety of further applications is envisaged and falls within the scope of the appended claims.
In the context of the present disclosure, the term skin-print is used to refer to a skin-print that is latent and/or residual. That is to say the skin-print is what is left behind on a surface once the human skin, from which the skin-print is derived, has been removed. The term skin-print is independent of the size and geometry of the substrate and/or the typical area of contact. As the skilled person would readily appreciate, for the purposes of chemical analysis of the skin-print it is important that the skin-print is not diluted for example by such material as ink which, while may assist for the purpose of visualising the skin-print, may compromise the chemical analysis.
| Number | Date | Country | Kind |
|---|---|---|---|
| 1802356.4 | Feb 2018 | GB | national |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/GB2019/050385 | 2/13/2019 | WO | 00 |
