Claims
- 1. A recombinant DNA construct comprising a tissue specific promoter, operably linked to a vacuole targeting sequence, operably linked to a coding sequence for a bacterial levansucrase gene wherein transformation of a plant cell selected from the group consisting of corn, potato, and tobacco with said construct results in production of fructan in the vacuole of said plant cell.
- 2. A plant selected from the group consisting of corn, potato, and tobacco transformed with the construct of claim 1 such that said plant produces fructan which accumulates in the vacuole of the cells of the plant.
- 3. A method of producing fructan comprising growing the plant of claim 2, harvesting said plant, and extracting said fructan from the harvested plant.
- 4. A recombinant DNA construct as described in claim 1 wherein the tissue specific promoter is specific to seed.
- 5. A recombinant DNA construct as described in claim 1 wherein the tissue specific promoter is specific to tubers.
- 6. A plant transformed with the construct of claim 4 wherein the plant produces fructan which accumulates in the seed.
- 7. A plant transformed with the construct of claim 5 wherein the plant produces fructan which accumulates in the tuber.
- 8. A method of increasing fructan levels in plants, comprising transforming a plant cell that has a higher than native sucrose level with a recombinant DNA construct comprising a tissue specific promoter, operably linked to a vacuole targeting sequence, operably linked to a coding sequence of a bacterial levansucrase gene, and growing a fertile, sexually mature, transformed plant from the transformed plant cell, wherein the transformed plant synthesizes and accumulates fructan.
- 9. A transformed plant produced by the method of claim 8.
- 10. A seed produced by the transformed plant of claim 9.
- 11. The method of claim 8 wherein the transformed plant is corn.
CROSS-REFERENCE TO RELATED APPLICATIONS
This is the national stage of international application PCT/US94/12778, filed Nov. 7, 1994, which is a continuation-in-part of U.S. patent application Ser. No. 08/149,689, filed Nov. 9, 1993 now abandoned.
PCT Information
Filing Document |
Filing Date |
Country |
Kind |
102e Date |
371c Date |
PCT/US94/12778 |
11/7/1994 |
|
|
5/6/1996 |
5/6/1996 |
Publishing Document |
Publishing Date |
Country |
Kind |
WO95/13389 |
5/18/1995 |
|
|
US Referenced Citations (4)
Number |
Name |
Date |
Kind |
4277563 |
KIerkhoffs |
Jul 1981 |
|
4734402 |
Hashimoto et al. |
Mar 1988 |
|
4788065 |
Nakamura et al. |
Nov 1988 |
|
5502273 |
Bright et al. |
Mar 1996 |
|
Foreign Referenced Citations (1)
Number |
Date |
Country |
WO8912386 |
Dec 1989 |
WOX |
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
149689 |
Nov 1993 |
|