Acetylenes disubstituted with a 5 substituted tetrahydronaphthyl group and with an aryl or heteroaryl group having retinoid-like biological activity

Description

1. FIELD OF THE INVENTION
The present invention relates to novel compounds having retinoid-like activity. More specifically, the present invention relates to compounds having an acetylene portion which is substituted with a 5 substituted tetrahydronaphthyl and by a substituted aryl or substituted heteroaryl group having an acid function. The acid function may also be converted to an alcohol, aldehyde or ketone or derivatives thereof, or may be reduced to --CH.sub.3.
2. BACKGROUND ART
Compounds which have retinoid-like activity are well known in the art, and are described in numerous United States and other patents and in scientific publications. It is generally known and accepted in the art that retinoid-like activity is useful for treating animals of the mammalian species, including humans, for curing or alleviating the symptoms and conditions of numerous diseases and conditions. In other words, it is generally accepted in the art that pharmaceutical compositions having a retinoid-like compound or compounds as the active ingredient are useful as regulators of cell proliferation and differentiation, and particularly as agents for treating skin-related diseases, including, actinic keratoses, arsenic keratoses, inflammatory and non-inflammatory acne, psoriasis, ichthyoses and other keratinization and hyperproliferative disorders of the skin, eczema, atopic dermatitis, Darriers disease, lichen planus, prevention and reversal of glucocorticoid damage (steroid atrophy), as a topical anti-microbial, as skin anti-pigmentation agents and to treat and reverse the effects of age and photo damage to the skin. Retinoid compounds are also useful for the prevention and treatment of cancerous and precancerous conditions, including, premalignant and malignant hyperproliferative diseases such as cancers of the breast, skin, prostate, cervix, uterus, colon, bladder, esophagus, stomach, lung, larynx, oral cavity, blood and lymphatic system, metaplasias, dysplasias, neoplasias, leukoplakias and papillomas of the mucous membranes and in the treatment of Kaposi's sarcoma. In addition, retinoid compounds can be used as agents to treat diseases of the eye, including, without limitation, proliferative vitreoretinopathy (PVR), retinal detachment, dry eye and other corneopathies, as well as in the treatment and prevention of various cardiovascular diseases, including, without limitation, diseases associated with lipid metabolism such as dyslipidemias, prevention of post-angioplasty restenosis and as an agent to increase the level of circulating tissue plasminogen activator (TPA). Other uses for retinoid compounds include the prevention and treatment of conditions and diseases associated with human papilloma virus (HPV), including warts and genital warts, various inflammatory diseases such as pulmonary fibrosis, ileitis, colitis and Krohn's disease, neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease and stroke, improper pituitary function, including insufficient production of growth hormone, modulation of apoptosis, including both the induction of apoptosis and inhibition of T-Cell Cell activated apoptosis, restoration of hair growth, including combination therapies with the present compounds and other agents such as Minoxidil.RTM., diseases associated with the immune system, including use of the present compounds as immunosuppressants and immunostimulants, modulation of organ transplant rejection and facilitation of wound healing, including modulation of chelosis.
U.S. Pat. Nos. 4,740,519 (Shroot et al.), 4,826,969 (Maignan et al.), 4,326,055 (Loeliger et al.), 5,130,335 (Chandraratna et al.), 5,037,825 (Klaus et al.), 5,231,113 (Chandraratna et al.), 5,324,840 (Chandraratna), Published European Patent Application Nos. 0 176 034 A (Wuest et al.), 0 350 846 A (Klaus et al.), 0 176 032 A (Frickel et al.), 0 176 033 A (Frickel et al.), 0 253 302 A (Klaus et al.), 0 303 915 A (Bryce et al.), UK Patent Application GB 2190378 A (Klaus et al.), German Patent Application Nos. DE 3715955 A1 (Klaus et al.), DE 3602473 A1 (Wuest et al., and the articles J. Amer. Acad. Derm. 15: 756-764 (1986) (Sporn et al.), Chem. Pharm. Bull. 33:404-407 (1985) (Shudo et al.), J. Med Chem. 1988 31, 2182-2192 (Kagechika et al.), Chemistry and Biology of Synthetic Retinoids CRC Press Inc. 1990 p 334-335, 354 (Dawson et al.), describe or relate to compounds which include a tetrahydronaphthyl moiety and have retinoid-like or related biological activity. U.S. Pat. No. 4,391,731 (Boller et al.) describes tetrahydronaphthalene derivatives which are useful in liquid crystal compositions. Several co-pending applications and recently issued patents which are assigned to the assignee of the present application, are directed to further compounds having retinoid-like activity.
SUMMARY OF THE INVENTION
The present invention covers compounds of Formula ##STR2## Formula 3 wherein R.sub.1 is hydrogen or alkyl of 1 to 10 carbons;
R.sub.2 and R.sub.3 are hydrogen, or alkyl of 1 to 6 carbons and the substituted ethynyl group occupies either the 2 or the 3 position of the tetrahydronaphthalene nucleus;
m is an integer having the value of 0-3;
o is an integer having the value 0-4;
Y is a phenyl group, or heteroaryl selected from a group consisting of pyridyl, thienyl, furyl, pyridazinyl, pyrimidinyl, pyrazinyl, thiazolyl, oxazolyl, and imidazolyl, said groups being optionally substituted with one or two R.sub.2 groups;
A is (CH.sub.2).sub.n where n is 0-5, lower branched chain alkyl having 3-6 carbons, cycloalkyl having 3-6 carbons, alkenyl having 2-6 carbons and 1 or 2 double bonds, alkynyl having 2-6 carbons and 1 or 2 triple bonds;
B is hydrogen, COOH or a pharmaceutically acceptable salt thereof, COOR.sub.8, CONR.sub.9 R.sub.10, --CH.sub.2 OH, CH.sub.2 OR.sub.11, CH.sub.2 OCOR.sub.11, CHO, CH(OR.sub.12).sub.2, CHOR.sub.13 O, --COR.sub.7, CR.sub.7 (OR.sub.12).sub.2, CR.sub.7 OR.sub.13 O, or tri-lower alkylsilyl, where R.sub.7 is an alkyl, cycloalkyl or alkenyl group containing 1 to 5 carbons, R.sub.8 is an alkyl group of 1 to 10 carbons or trimethylsilylalkyl where the alkyl group has 1 to 10 carbons, or a cycloalkyl group of 5 to 10 carbons, or R.sub.8 is phenyl or lower alkylphenyl, R.sub.9 and R.sub.10 independently are hydrogen, an alkyl group of 1 to 10 carbons, or a cycloalkyl group of 5-10 carbons, or phenyl or lower alkylphenyl, R.sub.11 is lower alkyl, phenyl or lower alkylphenyl, R.sub.12 is lower alkyl, and R.sub.13 is divalent alkyl radical of 2-5 carbons, and
R.sub.19 is independently hydrogen, alkyl of 1 to 10 carbons, fluoro-substituted alkyl of 1 to 10 carbons, alkenyl of 2 to 10 carbons and having 1 to 3 double bonds, alkynyl having 2 to 10 carbons and 1 to 3 triple bonds, carbocyclic aryl selected from the group consisting of phenyl, C.sub.1 -C.sub.10 -alkylphenyl, naphthyl, C.sub.1 -C.sub.10 -alkylnaphthyl, phenyl-C.sub.1 -C.sub.10 alkyl, naphthyl-C.sub.1 -C.sub.10 alkyl; CN, CHO, CH(OR.sub.12).sub.2, CHOR.sub.13 O, (CH.sub.2).sub.p CO.sub.2 R.sub.8, (CH.sub.2).sub.p CH.sub.2 OH, (CH.sub.2).sub.p CH.sub.2 OR.sub.11, (CH.sub.2).sub.p CH.sub.2 OCOR.sub.11, where p is an integer between 0 to 10, or the two R.sub.19 groups jointly represent 3 to 6 methylene groups which together with the alkylidene carbon complete a ring.
In a second aspect, this invention relates to the use of the compounds of Formula 3 for the treatment of skin-related diseases, including, without limitation, actinic keratoses, arsenic keratoses, inflammatory and non-inflammatory acne, psoriasis, ichthyoses and other keratinization and hyperproliferative disorders of the skin, eczema, atopic dermatitis, Darriers disease, lichen planus, prevention and reversal of glucocorticoid damage (steroid atrophy), as a topical anti-microbial, as skin anti-pigmentation agents and to treat and reverse the effects of age and photo damage to the skin. The compounds are also useful for the prevention and treatment of cancerous and precancerous conditions, including, premalignant and malignant hyperproliferative diseases such as cancers of the breast, skin, prostate, cervix, uterus, colon, bladder, esophagus, stomach, lung, larynx, oral cavity, blood and lymphatic system, metaplasias, dysplasias, neoplasias, leukoplakias and papillomas of the mucous membranes and in the treatment of Kaposi's sarcoma. In addition, the present compounds can be used as agents to treat diseases of the eye, including, without limitation, proliferative vitreoretinopathy (PVR), retinal detachment, dry eye and other corneopathies, as well as in the treatment and prevention of various cardiovascular diseases, including, without limitation, diseases associated with lipid metabolism such as dyslipidemias, prevention of post-angioplasty restenosis and as an agent to increase the level of circulating tissue plasminogen activator (TPA). Other uses for the compounds of the present invention include the prevention and treatment of conditions and diseases associated with Human papilloma virus (HPV), including warts and genital warts, various inflammatory diseases such as pulmonary fibrosis, ileitis, colitis and Krohn's disease, neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease and stroke, improper pituitary function, including insufficient production of growth hormone, modulation of apoptosis, including both the induction of apoptosis and inhibition of T--Cell activated apoptosis, restoration of hair growth, including combination therapies with the present compounds and other agents such as Minoxidil.RTM., diseases associated with the immune system, including use of the present compounds as immunosuppressants and immunostimulants, modulation of organ transplant rejection and facilitation of wound healing, including modulation of chelosis.
This invention also relates to a pharmaceutical formulation comprising a compound of Formula 3 in admixture with a pharmaceutically acceptable excipient.
In another aspect, this invention relates to processes for making a compound of Formula 3 which process comprises reacting a compound of Formula 8 with a compound of Formula 9, in the presence of cuprous iodide and Pd(PQ.sub.3).sub.2 Cl.sub.2 (Q is phenyl) or a similar complex, or reacting the zinc salt of the compound shown in Formula 8 with a compound of Formula 9 in the presence of Pd(PQ.sub.3).sub.4 (Q is phenyl) or similar complex. In Formula 8 the symbol STHN (substituted tetrahydronaphthyl) represents a tetrahydronaphthalene nucleus which is appropriately substituted to provide the compounds defined in Formula 3 or said tetrahydronaphthalene nucleus is appropriately substituted to provide such precursors of compounds of Formula 3 from which the target compounds can be readily obtained by organic reactions well known in the art. In Formula 9 X.sub.1 is halogen, B' is H, or a protected acid, alcohol, aldehyde, or ketone. In effect, B' is either the desired B group of Formula 3 or B' is a precursor from which the B group can be readily obtained by reactions well known in the art. ##STR3##
Still further, the present invention relates to such reactions performed on the compounds of Formula 3 which cause transformations of the A-B group or of the substituents on the tetrahydronaphthalene moiety, while the reaction product still remains within the scope of Formula 3.
General Embodiments
Definitions
The term alkyl refers to and covers any and all groups which are known as normal alkyl, branched-chain alkyl and cycloalkyl. The term alkenyl refers to and covers normal alkenyl, branch chain alkenyl and cycloalkenyl groups having one or more sites of unsaturation. Similarly, the term alkynyl refers to and covers normal alkynyl, and branch chain alkynyl groups having one or more triple bonds.
Lower alkyl means the above-defined broad definition of alkyl groups having 1 to 6 carbons in case of normal lower alkyl, and as applicable 3 to 6 carbons for lower branch chained and cycloalkyl groups. Lower alkenyl is defined similarly having 2 to 6 carbons for normal lower alkenyl groups, and 3 to 6 carbons for branch chained and cyclo-lower alkenyl groups. Lower alkynyl is also defined similarly, having 2 to 6 carbons for normal lower alkynyl groups, and 4 to 6 carbons for branch chained lower alkynyl groups.
The term "ester" as used here refers to and covers any compound falling within the definition of that term as classically used in organic chemistry. It includes organic and inorganic esters. Where B (of Formula 3) is --COOH, this term covers the products derived from treatment of this function with alcohols or thiols preferably with aliphatic alcohols having 1-6 carbons. Where the ester is derived from compounds where B is --CH.sub.2 OH, this term covers compounds derived from organic acids capable of forming esters including phosphorous based and sulfur based acids, or compounds of the formula --CH.sub.2 OCOR.sub.11 where R.sub.11 is any substituted or unsubstituted aliphatic, aromatic, heteroaromatic or aliphatic aromatic group, preferably with 1-6 carbons in the aliphatic portions.
Unless stated otherwise in this application, preferred esters are derived from the saturated aliphatic alcohols or acids of ten or fewer carbon atoms or the cyclic or saturated aliphatic cyclic alcohols and acids of 5 to 10 carbon atoms. Particularly preferred aliphatic esters are those derived from lower alkyl acids and alcohols. Also preferred are the phenyl or lower alkyl phenyl esters.
Amides has the meaning classically accorded that term in organic chemistry. In this instance it includes the unsubstituted amides and all aliphatic and aromatic mono- and di-substituted amides. Unless stated otherwise in this application, preferred amides are the mono- and di-substituted amides derived from the saturated aliphatic radicals of ten or fewer carbon atoms or the cyclic or saturated aliphatic-cyclic radicals of 5 to 10 carbon atoms. Particularly preferred amides are those derived from substituted and unsubstituted lower alkyl amines. Also preferred are mono- and disubstituted amides derived from the substituted and unsubstituted phenyl or lower alkylphenyl amines. Unsubstituted amides are also preferred.
Acetals and ketals include the radicals of the formula--CK where K is (--OR) 2. Here, R is lower alkyl. Also, K may be --OR.sub.7 O-- where R.sub.7 is lower alkyl of 2-5 carbon atoms, straight chain or branched.
A pharmaceutically acceptable salt may be prepared for any compounds in this invention having a functionality capable of forming such salt, for example an acid functionality. A pharmaceutically acceptable salt is any salt which retains the activity of the parent compound and does not impart any deleterious or untoward effect on the subject to which it is administered and in the context in which it is administered.
Pharmaceutically acceptable salts may be derived from organic or inorganic bases. The salt may be a mono or polyvalent ion. Of particular interest are the inorganic ions, sodium, potassium, calcium, and magnesium. Organic salts may be made with amines, particularly ammonium salts such as mono-, di- and trialkyl amines or ethanol amines. Salts may also be formed with caffeine, tromethamine and similar molecules. Where there is a nitrogen sufficiently basic as to be capable of forming acid addition salts, such may be formed with any inorganic or organic acids or alkylating agent such as methyl iodide. Preferred salts are those formed with inorganic acids such as hydrochloric acid, sulfuric acid or phosphoric acid. Any of a number of simple organic acids such as mono-, di- or tri-acid may also be used.
Some of the compounds of the present invention may have trans and cis (E and Z) isomers. In addition, the compounds of the present invention may contain one or more chiral centers and therefore may exist in enantiomeric and diastereomeric forms. The scope of the present invention is intended to cover all such isomers per se, as well as mixtures of cis and trans isomers, mixtures of diastereomers and racemic mixtures of enantiomers (optical isomers) as well. In the present application when no specific mention is made of the configuration (cis, trans or R or S) of a compound (or of an asymmetric carbon) then a mixture of such isomers, or either one of the isomers is intended. In a similar vein, when in the chemical structural formulas of this application a straight line representing a valence bond is drawn to an asymmetric carbon, then isomers of both R and S configuration, as well as their mixtures are intended. Defined stereochemistry about an asymmetric carbon is indicated in the formulas (where applicable) by a solid triangle showing .beta. configuration, or by a hashed line showing .alpha. configuration.
Referring now to the nomenclature used in naming the compounds of the invention and intermediate compounds leading thereto, two different systems for numbering the tetrahydronaphthalene ring are demonstrated as shown by the structural formulas of Compounds F, G and 1. Compound 1 and Compounds F and G are exemplary intermediates utilized in the synthesis of the compounds of the invention. The numbering systems illustrated here will not only be readily apparent to those skilled in the art, but will be readily understood as it is applied in the ensuing description of the compounds of the invention and of intermediates utilized for obtaining the compounds of the invention. ##STR4##
With reference to the symbol Y in Formula 3, the preferred compounds of the invention are those where Y is phenyl, pyridyl, thienyl or furyl. Even more preferred are compounds where Y is phenyl or pyridyl. As far as substititutions on the Y (phenyl) and Y (pyridyl) groups are concerned, compounds are preferred where the phenyl group is 1,4 (para) substituted, and where the pyridine ring is 2,5 substituted. (Substitution in the 2,5 positions in the "pyridine" nomenclature corresponds to substitution in the 6-position in the "nicotinic acid" nomenclature.) In the preferred compounds of the invention there is no optional R.sub.2 substituent on the Y group.
The A-B group of the preferred compounds is (CH.sub.2).sub.n --COOH or (CH.sub.2).sub.n --COOR.sub.8 where R.sub.8 is defined as above. Even more preferably n is zero and R.sub.8 is lower alkyl.
Referring still to the preferred compounds of Formula 3, the aromatic portion of the tetrahydronaphthalene moiety is preferably substituted only by the acetylene function. In other words, in the preferred compounds there is no R.sub.2 substituent (other than hydrogen). Similarly, in the preferred compounds of the invention there is no R.sub.3 substituent (other than hydrogen). The R.sub.1 substituent of the compounds of the invention is preferably lower alkyl, and even more preferably methyl.
The R.sub.19 groups of the compounds of the invention preferably are hydrogen, alkyl of 1 to 10 carbons, cyano (CN) or COOR.sub.8. Even more preferably the R.sub.19 groups are H, CN, COOEt or lower alkyl. Alternatively, in the preferred compounds of the invention in accordance with Formula 3 the two R.sub.19 groups jointly form a --(CH.sub.2).sub.q -- radical, (q is an integer having the values of 3 to 7) whereby a cycloalkyl ring is formed, most preferably a cyclohexyl ring. Specific preferred compounds in accordance with Formula 3 and their synthesis are described below in the section of this application titled "Specific Examples". The presently most preferred compounds of the invention in accordance with Formula 3 are indicated in Table 1 below, with reference to Formula 3A. In this Table the tetrahydronaphthalene ring is numbered as shown above in connection with Compound 1. ##STR5##
TABLE 1______________________________________ Position of EthynylCompound No. Subst. X.sub.2 R.sub.8 R.sub.19' R.sub.19"______________________________________115 2 CH Et H CO.sub.2 Et121 3 CH Et H CO.sub.2 Et131 2 CH Et (CH.sub.2).sub.5.sup.1 --132 3 CH Et (CH.sub.2).sub.5.sup.1 --133 2 CH Et Et Et134 3 CH Et Et Et135 2 CH H (CH.sub.2).sub.5.sup.1 --136 3 CH H (CH.sub.2).sub.5.sup.1 --137 2 CH H Et Et138 3 CH H Et Et145 2 CH Et CN.sup.2 H146 2 CH Et H CN.sup.3______________________________________ .sup.1 The R'.sub.19 and R.sub.19 " groups jointly represent methylene groups which together with the alkylidene carbon complete a ring having a total of 6 carbons. .sup.2 The CN group is in the E (trans) configuration. .sup.3 The CN group is in Z (cis) configuration.
Modes of Administration
The compounds of this invention may be administered systemically or topically, depending on such considerations as the condition to be treated, need for site-specific treatment, quantity of drug to be administered, and numerous other considerations.
In the treatment of dermatoses, it will generally be preferred to administer the drug topically, though in certain cases such as treatment of severe cystic acne or psoriasis, oral administration may also be used. Any common topical formulation such as a solution, suspension, gel, ointment, or salve and the like may be used. Preparation of such topical formulations are well described in the art of pharmaceutical formulations as exemplified, for example, Remington's Pharmaceutical Science, Edition 17, Mack Publishing Company, Easton, Pa. For topical application, these compounds could also be administered as a powder or spray, particularly in aerosol form. If the drug is to be administered systemically, it may be confected as a powder, pill, tablet or the like or as a syrup or elixir suitable for oral administration. For intravenous or intraperitoneal administration, the compound will be prepared as a solution or suspension capable of being administered by injection. In certain cases, it may be useful to formulate these compounds by injection. In certain cases, it may be useful to formulate these compounds in suppository form or as extended release formulation for deposit under the skin or intramuscular injection.
Other medicaments can be added to such topical formulation for such secondary purposes as treating skin dryness; providing protection against light; other medications for treating dermatoses; medicaments for preventing infection, reducing irritation, inflammation and the like.
Treatment of dermatoses or any other indications known or discovered to be susceptible to treatment by retinoic acid-like compounds will be effected by administration of the therapeutically effective dose of one or more compounds of the instant invention. A therapeutic concentration will be that concentration which effects reduction of the particular condition, or retards it expansion. In certain instances, the compound potentially may be used in prophylactic manner to prevent onset of a particular condition.
A useful therapeutic or prophylactic concentration will vary from condition to condition and in certain instances may vary with the severity of the condition being treated and the patient's susceptibility to treatment. Accordingly, no single concentration will be uniformly useful, but will require modification depending on the particularities of the disease being treated. Such concentrations can be arrived at through routine experimentation. However, it is anticipated that in the treatment of, for example, acne, or similar dermatoses, that a formulation containing between 0.01 and 1.0 milligrams per milliliter of formulation will constitute a therapeutically effective concentration for total application. If administered systemically, an amount between 0.01 and 5 mg per kg per day of body weight would be expected to effect a therapeutic result in the treatment of many diseases for which these compounds are useful.
Assay of Retinoid-like Biological Activity
The retinoic acid-like activity of these compounds is confirmed through the classic measure of retinoic acid activity involving the effects of retinoic acid on ornithine decarboxylase. The original work on the correlation between retinoic acid and decrease in cell proliferation was done by Verma & Boutwell, Cancer Research, 1977, 37,2196-2201. That reference discloses that ornithine decarboxylase (ODC) activity increased precedent to polyamine biosynthesis. It has been established elsewhere that increases in polyamine synthesis can be correlated or associated with cellular proliferation. Thus, if ODC activity could be inhibited, cell hyperproliferation could be modulated. Although all cases for ODC activity increases are unknown, it is known that 12-0-tetradecanoylphorbol-13-acetate (TPA) induces ODC activity. Retinoic acid inhibits this induction of ODC activity by TPA. An assay essentially following the procedure set out in Cancer Research: 1662-1670,1975 may be used to demonstrate inhibition of TPA induction of ODC by compounds of this invention. Activity of exemplary compounds of the present invention in the above-described ODC assay is disclosed in Table 2 which provides the IC.sub.80 concentration for the respective exemplary compound. ("IC.sub.80 " is that concentration of the test compound which causes 80% inhibition in the ODC assay. By analogy, "IC.sub.60, for example, is that concentration of the test compound which causes 60% inhibition in the ODC assay.)
TABLE 2______________________________________Compound # IC.sub.80 conc (nmols)______________________________________115 3.90121 2.50131 19.10133 0.30134 10* (IC.sub.48.6)145 6.60______________________________________ *the inhibition shown in brackets was attained at this concentration;
SPECIFIC EMBODIMENTS
The compounds of this invention can be made by the synthetic chemical pathways illustrated here. The synthetic chemist will readily appreciate that the conditions set out here are specific embodiments which can be generalized to any and all of the compounds of the invention.
SYNTHESIS ##STR6##
Referring now to Reaction Scheme 1 a synthetic route leading to precursors of the compounds of the invention, is illustrated. In accordance with this scheme, a 6- or 7-bromo substituted 3,4-dihydro-naphthalen-1(2H)-one (numbering as shown for Compound G) of Formula 10 is the starting material. The compounds of Formula 10 already carry the desired R.sub.1, R.sub.2 and R.sub.3 substituents, as these are defined above in connection with Formula 3. The compounds of Formula 10 are reacted with trimethylsilylacetylene to provide the 6- or 7-trimethylsilylethynyl- substituted 3,4-dihydro-naphthalen-1(2H)-one compounds of Formula 11. The reaction with trimethylsilylacetylene is typically conducted under heat (approximately 100.degree. C.) in the presence of cuprous iodide, a suitable catalyst, typically having the formula Pd(PPh.sub.3).sub.2 C.sub.12, an acid acceptor (such as triethylamine) under an inert gas (argon) atmosphere. Typical reaction time is approximately 24 hours. The 6- or 7-(trimethylsilyl)ethynyl- substituted 3,4-dihydro-naphthalen-1(2H)-one compounds of Formula 11 are then reacted with base (potassium hydroxide or potassium carbonate) in an alcoholic solvent, such as methanol, to provide the 6- or 7-ethynyl substituted 3,4-dihydro-l-naphthalen-1(2H)ones of Formula 12. Compounds of Formula 12 are then coupled with the aromatic or heteroaromatic reagent X.sub.1 --Y(R.sub.2)--A-B' (Formula 9) in the presence of cuprous iodide, a suitable catalyst, typically Pd(PPh.sub.3).sub.2 Cl.sub.2, an acid acceptor, such as triethylamine, under inert gas (argon) atmosphere. Alternatively, a zinc salt (or other suitable metal salt) of the compounds of Formula 12 can be coupled with the reagents of Formula 9 in the presence of Pd(PPh.sub.3).sub.4 or similar complex. Typically, the coupling reaction with the reagent X.sub.1 --Y(R.sub.2)--A-B, (Formula 9) is conducted at room or moderately elevated temperature. Generally speaking, coupling between an ethynylaryl derivative or its zinc salt and a halogen substituted aryl or heteroaryl compound, such as the reagent of Formula 9, is described in U.S. Pat. No. 5,264,456, the specification of which is expressly incorporated herein by reference. The compounds of Formula 13 when appropriately protected in the B' group, can be reacted in a McMurry coupling reaction to provide compounds of the invention. The compounds of Formula 13 can also be converted into further precursors for the compounds of the invention by such reactions and transformations which are well known in the art. Such reactions are indicated in Reaction Scheme 1 by conversion into "homologs and derivatives". One such conversion employed for the synthesis of several exemplary compounds of this invention is saponification of an ester group (when B or B' is an ester) to provide the free carboxylic acid or its salt.
The halogen substituted aryl or heteroaryl compounds of Formula 9 can, generally speaking, be obtained by reactions well known in the art. An example of such compound is ethyl 4-iodobenzoate which is obtainable, for example, by esterification of 4-iodobenzoic acid. Another example is ethyl 6-iodonicotinoate which can be obtained by conducting a halogen exchange reaction on 6-chloronicotinic acid, followed by esterification. Even more generally speaking, regarding derivatization of compounds of Formula 13 and/or the synthesis of aryl and heteroaryl compounds of Formula 9 which can thereafter be reacted with compounds of Formula 12 to yield compounds of the invention or precursors thereto, the following well known and published general principles and synthetic methodology can be employed.
Carboxylic acids are typically esterified by refluxing the acid in a solution of the appropriate alcohol in the presence of an acid catalyst such as hydrogen chloride or thionyl chloride. Alternatively, the carboxylic acid can be condensed with the appropriate alcohol in the presence of dicyclohexylcarbodiimide and dimethylaminopyridine. The ester is recovered and purified by conventional means. Acetals and ketals are readily made by the method described in March, "Advanced Organic Chemistry," 2nd Edition, McGraw-Hill Book Company, p 810). Alcohols, aldehydes and ketones all may be protected by forming respectively, ethers and esters, acetals or ketals by known methods such as those described in McOmie, Plenum Publishing Press, 1973 and Protecting Groups, Ed. Greene, John Wiley & Sons, 1981.
To increase the value of n in the compounds of Formula 9 before affecting the coupling reaction of Reaction Scheme 1 (where such compounds corresponding to Formula 9 are not available from a commercial source) aromatic or heteroaromatic carboxylic acids are subjected to homologation by successive treatment under Arndt-Eistert conditions or other homologation procedures. Alternatively, derivatives which are not carboxylic acids may also be homologated by appropriate procedures. The homologated acids can then be esterified by the general procedure outlined in the preceding paragraph.
Compounds of Formula 9, (or of the invention as set forth in Formula 3, or related intermediates, as applicable) where A is an alkenyl group having one or more double bonds can be made for example, by synthetic schemes well known to the practicing organic chemist; for example by Wittig and like reactions, or by introduction of a double bond by elimination of halogen from an alpha-halo-arylalkyl-carboxylic acid, ester or like carboxaldehyde. Compounds of Formula 9 (or of the invention as set forth in Formula 3 or intermediates, as applicable) where the A group has a triple (acetylenic) bond can be made by reaction of a corresponding aromatic methyl ketone with strong base, such as lithium diisopropyl amide, reaction with diethyl chlorophosphate and subsequent addition of lithium diisopropyl amide.
The acids and salts derived from compounds of Formula 13 (or other intermediates, or compounds of the invention as set forth in Formula 3, as applicable) are readily obtainable from the corresponding esters. Basic saponification with an alkali metal base will provide the acid. For example, an ester of Formula 13 (or other compounds of the invention as set forth in Formula 3) may be dissolved in a polar solvent such as an alkanol, preferably under an inert atmosphere at room temperature, with about a three molar excess of base, for example, lithium hydroxide or potassium hydroxide. The solution is stirred for an extended period of time, between 15 and 20 hours, cooled, acidified and the hydrolysate recovered by conventional means.
The amide may be formed by any appropriate amidation means known in the art from the corresponding esters or carboxylic acids. One way to prepare such compounds is to convert an acid to an acid chloride and then treat that compound with ammonium hydroxide or an appropriate amine. For example, the acid is treated with an alcoholic base solution such as ethanolic KOH (in approximately a 10% molar excess) at room temperature for about 30 minutes. The solvent is removed and the residue taken up in an organic solvent such as diethyl ether, treated with a dialkyl formamide and then a 10-fold excess of oxalyl chloride. This is all effected at a moderately reduced temperature between about -10 degrees and +10 degrees C. The last mentioned solution is then stirred at the reduced temperature for 1-4 hours, preferably 2 hours. Solvent removal provides a residue which is taken up in an inert organic solvent such as benzene, cooled to about 0 degrees C and treated with concentrated ammonium hydroxide. The resulting mixture is stirred at a reduced temperature for 1-4 hours. The product is recovered by conventional means.
Alcohols are made by converting the corresponding acids to the acid chloride with thionyl chloride or other means (J. March, "Advanced Organic Chemistry", 2nd Edition, McGraw-Hill Book Company), then reducing the acid chloride with sodium borohydride (March, Ibid, pg. 1124), which gives the corresponding alcohols. Alternatively, esters may be reduced with lithium aluminum hydride at reduced temperatures. Alkylating these alcohols with appropriate alkyl halides under Williamson reaction conditions (March, Ibid, pg. 357) gives the corresponding ethers. These alcohols can be converted to esters by reacting them with appropriate acids in the presence of acid catalysts or dicyclohexylcarbodiimide and dimethylaminopyridine.
Aldehydes can be prepared from the corresponding primary alcohols using mild oxidizing agents such as pyridinium dichromate in methylene chloride (Corey, E. J., Schmidt, G., Tet. Lett., 399, 1979), or dimethyl sulfoxide/oxalyl chloride in methylene chloride (Omura, K., Swern, D., Tetrahedron, 1978, 34, 1651).
Ketones can be prepared from an appropriate aldehyde by treating the aldehyde with an alkyl Grignard reagent or similar reagent followed by oxidation.
Acetals or ketals can be prepared from the corresponding aldehyde or ketone by the method described in March, Ibid, p 810.
Compounds of Formula 9 (or other intermediates or of the invention as set forth in Formula 3) where B is H can be prepared from the corresponding halogenated aromatic or hetero aromatic compounds, preferably where the halogen is I. ##STR7##
In the preferred compounds of the invention the two R.sub.1 substituents are methyl, and the R.sub.2 and R.sub.3 substituents are hydrogen. Reaction Scheme 2 illustrates a synthetic process for preparing 7-bromo-3,4-dihydro-4,4-dimethylnaphthalen-1-one (Compound G) which serves as a starting material for the synthesis of several preferred compounds of the invention. Thus, referring now specifically to Reaction Scheme 2, ethyl 3-bromophenylacetate (Compound B, made by esterification of 3-bromophenylacetic acid) is reduced with diisobutylaluminumhydride (DIBAL-H) to yield (3-bromophenyl)acetaldehyde. (3-Bromophenyl)acetaldehyde is reacted in a Wittig reaction with (carbethoxymethylene)triphenylphosphorane to provide a mixture of E and Z ethyl 4-(3-bromophenyl)but-2-enoates. The latter compounds are hydrogenated to yield ethyl 4-(3-bromophenyl)butanoate (Compound D). Compound D is reacted with the Grignard reagent derived from methylbromide to give the tertiary alcohol 5-(3-bromophenyl)-2-methylpentan-2-ol (Compound E) (It should be apparent to those skilled in the art, that the choice of the Grignard reagent used in this reaction step determines the nature of the R.sub.1 substituent in the resulting compounds of the invention.) Compound E is then treated with acid to cyclize it and to form 6-bromo-1,2,3,4-tetrahydro-1,1-dimethylnaphthalene (Compound F). Compound F is oxidized with chromium trioxide to yield 7-bromo-3,4-dihydro-4,4-dimethylnaphthalen-1(2H)-one (Compound G). Compound G is covered by Formula 10 and in accordance with Reaction Schemes 1 and 3 serves as a starting material in the synthesis of several preferred compounds of the invention.
6-Bromo-3,4-dihydro-4,4-dimethylnaphthalen-1(2H)-one (Compound H) is isomeric with Compound G, and can be obtained, starting with ethyl (4-bromophenyl)acetate, in accordance with the sequence of reactions illustrated in Reaction Scheme 2 for Compound G.
6-Bromo-3,4-dihydro-4,4-dimethylnaphthalen-1(2H)-one (Compound H) can also be obtained in accordance with the published literature procedure: Mathur et al. Tetrahedron, 41, 1509-1516 (1985). Compound H is also covered by Formula 10 and in accordance with Reaction Schemes 1 and 3 serves as a starting material in the synthesis of several preferred compounds of the invention.
Starting materials for the synthetic routes outlined in Reaction Schemes 1-3 where the R.sub.2 and/or R.sub.3 groups are other than hydrogen, can be obtained similarly to the synthesis of the starting materials demonstrated in Reaction Scheme 2, and/or by introducing the R.sub.2 by a Friedel--Crafts or like reaction into the aromatic portion of the tetrahydronaphthalene nucleus. ##STR8##
Compounds of Formula 3 are preferably synthesized in accordance with the synthetic steps illustrated in Reaction Schemes 3, 4 and 5. Referring first to Reaction Scheme 3, the 1-oxo 6- or 7-bromo 1,2,3,4-tetrahydronaphthalene derivative (numbering as exemplified for Compound G) of Formula 10 is reacted with a ketone or aldehyde compound of Formula 25 wherein the R.sub.19 groups are defined as in connection with Formula 3. The reaction (McMurry coupling) is conducted at elevated temperature in the presence of lithium metal and titanium trichloride, in an inert ether type solvent, for example in refluxing 1,2-dimethoxyethane (DME). The resulting compounds of Formula 26 are then reacted with trimethylsilylacetylene as described above in connection with Reaction Scheme 1 (cuprous iodide, Pd(PPh.sub.3).sub.2 Cl.sub.2, catalyst and triethylamine under inert atmosphere) and thereafter with base (potassium hydroxide or potassium carbonate in an alcoholic solvent, such as methanol) to provide the 6- or 7-ethynyl 1,2,3,4-tetrahydronaphthalene derivatives of Formula 27 which have the R.sub.19 R.sub.19 C=group attached to the tetrahydronaphthalene nucleus. Compounds of Formula 27 are then coupled with the aromatic or heteroaromatic reagent X.sub.1 --Y(R.sub.2)--A-B, (Formula 9) in the presence of cuprous iodide, a suitable catalyst, typically Pd(PPh.sub.3).sub.2 Cl.sub.2, an acid acceptor, such as diethylamine, under inert gas (argon) atmosphere, as described above in connection with Reaction Scheme 1, to yield compounds of Formula 28. Alternatively, as is also described in connection with the analogous coupling reaction of Scheme 1, the zinc salts of the compounds of Formula 27 are coupled with the reagents of Formula 9, in the presence of Pd(PPh.sub.3).sub.4 or similar complex. Compounds of Formula 28 are within the scope of Formula 3. They can be converted to further homologs and derivatives which are still within the scope of the invention, as is described above in connection with compounds of Formula 13. ##STR9##
Referring now to Reaction Scheme 4, synthesis of those compounds of Formula 3 is illustrated where one of the R.sub.19 groups represents hydrogen and the other is cyano (CN). These compounds are obtained by reacting a 5-oxo 2- or 3-(aryl or heteroaryl)ethynyl 5,6,7,8-tetrahydronaphthalene compound of Formula 13 (numbering as exemplified for Compound 1) with diethyl cyanomethylphosphonate and potassium bis(trimethylsilyl)amide in an inert ether type solvent, such as tetrahydrofuran. The resulting compounds of Formula 29 can be converted into further homologs and derivatives, as described above. Also, the cyano derivative (Formula 29) can be converted into the corresponding aldehyde by reduction with diisobutylaluminum hydride. ##STR10##
Reaction Scheme 5 illustrates synthesis of those compounds of Formula 3 wherein one of the R.sub.19 groups is hydrogen and the other is a carboxylic acid ester (or derivative thereof). The starting compounds for these syntheses are compounds of Formula 30, which can be obtained by conducting a Reformatsky reaction (reaction with an e halocarboxylic acid in the presence of zinc, under conditions well known in the art) on the 5-oxo tetrahydronaphthalene compound of Formula 10 followed by the synthetic steps outlined in Reaction Scheme 1. Alternatively a compound of Formula 30 can also be obtained by conducting a Reformatsky reaction on a compound (suitably protected in the B' group) of Formula 13. Dehydration (elimination of the hydroxy group) of compounds of Formula 30 with a suitable reagent such as (methoxycarbonylsulfamoyl)triethylammonium hydroxide (Burgess reagent) by heating moderately (50.degree. C.) in an inert solvent, such as benzene, provides compounds of Formula 31 and also compounds isomeric therewith where the newly formed double bond is within the ring. The latter isomeric compounds are not shown in Reaction Scheme 5. The mixtures of these isomers are separated by chromatography or other conventional methods known in the art, to yield the compounds of Formula 31. The compounds of Formula 31 are within the scope of Formula 3, and can also be converted into further homologs and derivatives still within the scope of the invention.

SPECIFIC EXAMPLES
Ethyl (4-bromophenyl)acetate
(Compound A)
A solution of 43 g (200 mmol) of 4bromophenylacetic acid and 0.2 g of conc. H2SO.sub.4 in 470 ml of ethanol was refluxed for 16 hours. The reaction mixture was cooled to ambient temperature, stirred with 6 g of solid K.sub.2 CO.sub.3 for 30 minutes and then filtered. The filtrate was concentrated in vacuo, diluted with Et.sub.2 O (200 ml), washed with 10% aqueous NaHCO.sub.3 (10 ml) and brine (10 ml), dried over MgSO.sub.4 and concentrated in vacuo to give the title compound as a colorless oil.
PMR (CDCl.sub.3): .delta.1.25 (3H, t, J=7.0 Hz), 3.56 (2H, s), 4.15 (2H, q, J=7.0 Hz), 7.16 (2H, d, J=8.4 Hz), 7.45 (2H, d, J=8.4 Hz).
Ethyl (3-bromophenyl)acetate
(Compound B)
Employing the same general procedure as for the preparation of ethyl (4-bromophenyl)acetate (Compound A), 100 g (463 mmol) of 3-bromophenylacetic acid was converted into the title compound (yellow oil) using 2 g of conc. H.sub.2 SO.sub.4 and 500 ml of ethanol.
PMR (CDCl.sub.3): .delta.1.26 (3H, t, J=7.0 Hz), 3.56 (2H, s), 4.16 (2H, q, J=7.0 Hz), 7.16-7.26 (2H, m), 7.38-7.46 (2H, m).
Ethyl 4-(4-bromophenyl)butanoate
(Compound C)
To a cold solution (-78.degree. C.) of 15 g (62 mmol) of ethyl (4-bromophenyl)acetate (Compound A) in 150 ml of CH.sub.2 Cl.sub.2 was added dropwise (over a span of 1 hour) 65 ml (65 mmol) of diisobutylaluminum hydride (DIBAL-H, 1M solution in hexane). After the DIBAL-H addition was complete, the reaction was stirred at -78.degree. C. for an additional hour. The reaction was quenched by the dropwise addition of methanol (10 ml), followed by water (10 ml) and 10% HCl (40 ml). The mixture was then warmed to 0.degree. C., stirred for 10 minutes and then washed with water (15 ml), 10% aqueous NaHCO.sub.3 (10 ml) and brine (10 ml). The organic phase was dried over MgSO.sub.4 and the solvent distilled off at ambient temperature to give crude (4-bromophenyl)acetaldehyde. To a cold solution (0.degree. C.) of this crude aldehyde in 150 ml of CH.sub.2 Cl.sub.2 was added a solution of 26 g (74.6 mmol) of (carbethoxymethylene)triphenylphosphorane in 50 ml of CH.sub.2 Cl.sub.2. The mixture was stirred for 16 hours, concentrated in vacuo and purified by flash chromatography (silica, 10% EtOAc-hexane) to give ethyl 4-(4-bromophenyl)but-2-enoate as a mixture of E:Z isomers. This isomeric mixture was dissolved in 150 ml of EtOAc and hydrogenated over 1 g of 10% Pd/C for 6 hours. The catalyst was filtered off and the filtrate concentrated in vacuo to give the title compound as a white solid.
PMR (CDCl.sub.3): .delta.1.26 (3H, t, J=7.1 Hz), 1.88-1.99 (2H, m), 2.31 (2H, t, J=7.5 Hz), 2.61 (2H, t, J=7.5 Hz), 4.28 (2H, q, J=7.1 Hz), 7.05 (2H, d, J=8.4 Hz), 7.40(2H, d, J=8.4 Hz).
Ethyl 4-(3-bromophenyl)butanoate
(Compound D)
Employing the same general multistep preparation as for ethyl 4-(4-bromophenyl)butanoate (Compound C), 60 g (246 mmol) of ethyl (3-bromophenyl)acetate (Compound B) was converted into the title compound (oil) using 255 ml (255 mmol) of diisobutylaluminum hydride (DIBAL-H, 1M in hexane), 85.8 g (250 mmol) of (carbethoxymethylene)triphenylphosphorane and 1.7 g of 0% Pd/C.
PMR (CDCl.sub.3): .delta.1.26 (3H, t, J=7.1 Hz), 1.89-2.00 (2H, m), 2.31 (2H, t, J=7.5 Hz), 2.63 (2H, t, J=7.2 Hz), 4.15 (2H, q, J=7.1 Hz), 7.10-7.35 (4H, m).
5-(3-bromophenyl)-2-methylpentan-2-ol
(Compound E)
To a cold solution (0.degree. C.) of 17 g (63 mmol) of ethyl 4-(3-bromophenyl)butanoate (Compound D) in 40 ml of THF was added 63 ml (189 mmol) of methylmagnesium bromide (3.0M solution in THF). The reaction was stirred at 0.degree. C. for 2 hours, quenched by the slow addition of ice cold water (30 ml) followed by 10% HCl (30 ml) and then extracted with Et.sub.2 O (4.times.60 ml). The combined organic layer was washed with 10% aqueuos NaHCO.sub.3 (10 ml), water (10 ml) and brine (10 ml), dried over MgSO.sub.4 and concentrated in vacuo. Purification by kugelrohr distillation gave the title compound as a colorless oil.
PMR (CDCl.sub.3): .delta.1.20 (6H, s), 1.43-1.55 (2H, m), 1.62-1.78 (2H, m), 2.60 (2H, t, J=6.0 Hz), 7.10-7.41 (4H, m).
6-Bromo-1,2,3,4-tetrahydro-1,1-dimethylnaphthalene
(Compound F)
15.0 g (58.3 mmol) of 5-(3-bromophenyl)-2-methyl-pentan-2-ol (Compound E) was cooled to 0.degree. C. and then 2.8 ml of conc. H.sub.2 SO.sub.4 was added. The mixture was stirred for 2.5 hours, diluted with water (20 ml) and extracted with Et.sub.2 O (3.times.40 ml). The combined organic layers were washed with water, sat. aqueous NaHCO.sub.3 and brine, dried over MgSO.sub.4 and concentrated in vacuo. Purification by kugelrohr distillation gave the title compound as a colorless oil.
PMR (CDCl.sub.3): .delta.1.25 (6H, s), 1.61-1.66 (2H, m), 1.74-1.82 (2H, m), 2.73 (2H, t, J=6.0 Hz), 7.16-7.26 (3H, m).
7-Bromo-3,4-dihydro-4,4-dimethyl-naphthalen-1(2H)-one
(Compound G)
To a cold mixture (0.degree. C.) of 209 g (200 mmol) of chromium trioxide, 100 ml (1.06 mol) of acetic anhydride and 200 ml (3.5 mol) of acetic acid was added a solution of 10 g (41.8 mmol) of 6-bromo-1,2,3,4-tetrahydro-1,1-dimethylnaphthalene (Compound F) in 125 ml of benzene. The reaction mixture was stirred for 1 hour, quenched with ice cold water and extracted with Et.sub.2 O (3.times.100 ml). The organic layer was dried over MgSO.sub.4, concentrated in vacuo, and purified by column chromatography (silica, 10% EtOAc-hexane) to give the title compound as a white solid.
PMR (CDCl.sub.3): .delta.1.28 (6H, s), 2.01 (2H, t, J=6.0 Hz), 2.72 (2H, t, J=6.0 Hz), 7.31 (1H, d, J=9.0 Hz), 7.61 (1H, dd, J=3.0, 9.0 Hz), 8.11 (1H, d, J=3.0 Hz).
6-Bromo-3,4-dihydro-4,4-dimethyl naphthalen-1(2H)-one
(Compound H)
Employing a published procedure (Mathur, N. C.; Snow, M. S. ; Young, K. M. ; and Pincock, J. A. Tetrahedron, 41, 1509-1516 (1985)), ethyl 4-(4-bromophenyl)butanoate (Compound C) was converted into the title compound. Alternatively, the title compound can be obtained using similar reactions that were used to convert ethyl 4-(3-bromophenyl)butanoate (Compound D) into 7-bromo-3,4-dihydro-4,4-dimethyl-naphthalen-1(2H)-one (Compound G).
6-Ethynyl-3,4-dihydro-4,4-dimethyl-naphthalen-1(2H)-one
(Compound K)
To a solution (flushed for 15 minutes with a stream of argon) of 13.55 g (53.8 mmol) of 6-bromo-3,4-dihydro-4,4-dimethyl-naphthalen-1(2H)-one (Compound H) in 280 ml of triethylamine was added 1.87 g (2.66 mmol) of bis(triphenylphosphine)palladium(II) chloride and 0.53 g (2.66 mmol) of cuprous iodide. The solution mixture was flushed with argon for 5 minutes and then 100 ml (938.7 mmol) of trimethylsilyl acetylene was added. The reaction mixture was sealed in a pressure tube and placed in a preheated oil bath (100.degree. C.) for 24 hours. The reaction mixture was then filtered through celite, washed with Et.sub.2 O and the filtrate concentrated in vacuo to give crude 6-(trimethylsilyl)ethynyl-3,4-dihydro-4,4-dimethyl-naphthalen-1(2H)-one. To a solution of this crude TMS-acetylenic compound in 50 ml of methanol was added 2.8 g (20.3 mmol) of K.sub.2 CO.sub.3. The mixture was stirred for 8 hours at ambient temperature and then filtered. The filtrate was concentrated in vacuo, diluted with Et.sub.2 O (100 ml), washed with water (10 ml), 10% HCl (10 ml) and brine (10 ml), dried over MgSO.sub.4 and concentrated in vacuo. Purification by column chromatography (silica, 10% EtOAc-hexane) yielded the title compound as a white solid.
PMR (CDCl.sub.3): .delta.1.38 (6H, s), 2.01 (2H, t, J=7.1 Hz), 2.72 (2H, t, J=7.1 Hz), 3.24 (1H, s), 7.39 (1H, dd, J=1.5, 8.1 Hz), 7.54 (1H, d, J=1.5 Hz), 7.91 (1H, d, J=8.1 Hz).
7-Ethynyl-3,4-dihydro-4,4-dimethyl-naphthalen-1(2H)-one
(Compound L)
Employing the same general procedure as for the preparation of 6-ethynyl-3,4-dihydro-4,4-dimethyl- naphthalen-1(2H)-one (Compound K), 7 g (27.6 mmol) of 7-bromo-3,4-dihydro-4,4-dimethyl-naphthalen-1(2H)-one (Compound G) was converted into the title compound using 39 ml (36.6 mmol) of trimethylsilyl acetylene, 0.97 g (1.3 mmol) of bis(triphenylphosphine)palladium(II) chloride, 0.26 g (1.3 mmol) of cuprous iodide and 0.6 g (4.3 mmol) of K.sub.2 CO.sub.3.
PMR (CDCl.sub.3): .delta.1.39 (6H, s), 2.02 (2H, t, J=7.0 Hz), 2.73 (2H, t, J=7.0 Hz), 3.08 (1H, s), 7.39 (1H, d, J=8.2 Hz), 7.61 (1H, dd, J=1.8, 8.2 Hz), 8.14 (1H, d, J=9 1.8 Hz). 6-Bromo-1(2H)-cyclohexylidene-3,4-dihydro-4,4-dimethylnaphthalene
(Compound O)
To a slurry of 12.2 g (79 mmol) of titanium trichloride in 150 ml of 1,2-dimethoxyethane (DME) under argon atmosphere was added in small portions 1.92 g (227 mmol) of lithium wire. The mixture was refluxed for 1 hour, cooled to ambient temperature and then a mixture of 1.56 g (15.9 mmol) of cyclohexanone and 1.0 g (3.97 mmol) of 6-bromo-3,4-dihydro-4,4-dimethylnaphthalen-1(2H)-one (Compound R) in 50 ml of DME was added. The resultant reaction mixture was stirred at ambient temperature for 2 hours, refluxed for 16 hours, diluted with hexane (100 ml) and then filtered through florisil. Purification by flash chromatography (silica, 100% hexane) to give the title compound as a pale yellow oil.
PMR (CDCl.sub.3): .delta.1.26 (6H, s), 1.52-1.64 (8H, br s), 2.30 (2H, t, J=5.5 Hz), 2.42 (2H, t, J=5.5 Hz), 2.53 (2H, t, J=7.1Hz), 7.04 (1H, d, J=8.2 Hz), 7.23 (1H, dd, J=2.1, 8.2 Hz), 7.41 (1H, d, J=2.1 Hz).
7-Bromo-1(2H)-cyclohexylidene-3,4-dihydro-4,4-dimethylnaphthalene
(Compound P)
Employing the same general procedure as for the preparation of 6-bromo-1(2H)-cyclohexylidene-3,4-dihydro-4,4-dimethylnaphthalene (Compound O), 1.0 g (3.97 mmol) of 7-bromo-3,4-dihydro-4,4-dimethyl-naphthalen-1(2H)-one (Compound G) was converted into the title compound using 1.56 g (15.9 mmol) of cyclohexanone, 1.92 g (277 mmol) of lithium and 12.2 g (79.4 mmol) of titanium trichloride.
PMR (CDCl.sub.3): .delta.1.23 (6H, s), 1.50-1.65 (8H, m), 2.33 (2H, br s), 2.45 (2H, t, J=5.5 Hz), 2.50 (2H, t, J=7.1 Hz), 7.15 (d, J=8.1 Hz), 7.26 (1H, d, J=1.6 Hz), 7.29 (1H, br s).
6-Bromo-1-(3-pentylidene)-1,2,3,4-tetrahydro-4,4-dimethylnaphthalene
(Compound Q)
Employing the same general procedure as for the preparation of 6-bromo-1(2H)-cyclohexylidene-3,4-dihydro-4,4-dimethylnaphthalene (Compound O), 1.0 g (3.97 mmol) of 6-bromo-3,4-dihydro-4,4-dimethyl-naphthalen-1(2H)-one (Compound H) was converted into the title compound using 1.37 g (15.9 mmol) of 3-pentanone, 1.92 g (277 mmol) of lithium and 12.2 g (79.4 mmol) of titanium trichloride.
PMR (CDCl.sub.3): .delta.1.05 (3H, t, J=7.3 Hz), 1.13 (3H, t, J=7.2 Hz), 1.25 (6H, s), 1.63 (2H, t, J=7.1 Hz), 2.16-2.30 (4H, m), 2.50 (2H, t, J=7.1 Hz), 7.09 (1H, d, J=8.2 Hz), 7.25 (1H, dd, J=2.1 , 8.2 Hz), 7.41 (1H, d, J=2.1 Hz).
7-Bromo-1-(3-pentylidene)-1,2,3,4-tetrahydro-4,4-dimethylnaphthalene
(Compound R)
Employing the same general procedure as for the preparation of 6-bromo-1(2H)-cyclohexylidene-3,4-dihydro-4,4-dimethylnaphthalene (Compound O), 1.0 g (3.97 mmol) of 7-bromo-3,4-dihydro-4,4-dimethyl-naphthalen-1(2H)-one (Compound G) was converted into the title compound using 1.37 g (15.9 mmol) of 3-pentanone, 1.92 g (277 mmol) of lithium and 12.2 g (79.4 mmol) of titanium trichloride.
PMR (CDCl.sub.3): .delta.1.04 (3H, t, J=7.5 Hz), 1.14 (3H, t, J=7.5 Hz), 1.23 (6H, s), 1.63 (2H, t, J=7.1 Hz), 2.21 (2H, q, J=7.5 Hz), 2.29 (2H, q, J=7.5 Hz), 2.49 (2H, t, J=7.1 Hz), 7.15 (1H, d, J=8.3 Hz), 7.29 (1H, dd, J=2.2, 8.3 Hz), 7.36 (1H, d, J=2.2 Hz).
6-Ethynyl-1(2H)-cyclohexylidene-3,4-dihydro-4,4-dimethylnaphthalene
(Compound U)
Employing the same general procedure as for the preparation of 6-ethynyl-3,4-dihydro-4,4-dimethyl-naphthalen-1(2H)-one (Compound K), 530 mg (1.66 mmol) of 6-bromo-1(2H)-cyclohexylidene-3,4-dihydro-4,4-dimethylnaphthalene (Compound O) was converted into the title compound using 1.63 g (16.6 mmol) of trimethylsilylacetylene, 20 mg (0.08 mmol) of cuprous iodide, 60 mg (0.08 mmol) of bis(triphenylphosphine)palladium(II) chloride and 40 mg (0.3 mmol) of potassium carbonate.
PMR (CDCl.sub.3): .delta.1.26 (6H, s), 1.54-1.64 (8H, br s), 2.33 (2H, br s), 2.43 (2H, t, J=5.9 Hz), 2.53 (2H, t, J=7.0 Hz), 3.07 (1H, s), 7.13 (1H, d, J=7.9 Hz), 7.27 (1H, dd, J=1.7, 7.9 Hz), 7.44 (1H, d, J=1.7 Hz).
7-Ethynyl-1(2H)-cyclohexylidene-3,4-dihydro-4,4-dimethylnaphthalene
(Compound V)
Employing the same general procedure as for the preparation of 6-ethynyl-3,4-dihydro-4,4-dimethylnaphthalen-1(2H)-one (Compound K), 351 mg (1.1 mmol) of 7-bromo-1(2H)-cyclohexylidene-3,4-dihydro-4,4-dimethylnaphthalene (Compound P) was converted into the title compound using 1.1 g (11 mmol) of trimethylsilylacetylene, 10 mg (0.04 mmol) of cuprous iodide, 38 mg (0.05 mmol) of bis(triphenylphosphine)palladium(II) chloride and 35 mg (0.25 mmol) of potassium carbonate.
PMR (CDCl.sub.3): .delta.1.25 (6H, s), 1.50-1.67 (8H, m), 2.32 (2H, br s), 2.45 (2H, t, J=5.9 Hz), 2.51 (2H, t, J=7.0 Hz), 3.00 (1H, s), 7.24 (1H, d, J=7.0 Hz), 7.29 (1H, d, J=1.8 Hz), 7.30 (1H, br s).
6-Ethynyl-3,4-dihydro-1(2H )-(3-pentylidene)-4,4 -dimethylnaphthalene
(Compound W)
Employing the same general procedure as for the preparation of 6-ethynyl-3,4-dihydro-4,4-dimethylnaphthalen-1(2H)-one (Compound K), 530 mg (1.66 mmol) of 6-bromo-3,4-dihydro-1(2H)-(3-pentylidene)-4,4-dimethylnaphthalene (Compound Q) was converted into the title compound using 1.63 g (16.6 mmol) of trimethylsilylacetylene, 20 mg (0.08 mmol) of cuprous iodide, 60 mg (0.08 mmol) of bis(triphenylphosphine)palladium(II) chloride and 40 mg (0.3 mmol) of potassium carbonate.
PMR (CDCl.sub.3): .delta.1.04 (3H, t, J=7.6 Hz), 1.13 (3H, t, J=8.3 Hz), 1.24 (6H, s), 1.64 (2H, t, J=7.0 Hz), 2.15-2.32 (4H, m), 2.50 (2H, t, J=7.0 Hz), 3.07 (1H, s), 7.18 (1H, d, J=8.0 Hz), 7.27 (1H, dd, J=1.7, 8.0 Hz), 7.43 (1H, d, J=1.7 Hz).
7-Ethynyl-3,4-dihydro-1(2H)-(3-pentylidene)-4,4-dimethylnaphthalene
(Compound X)
Employing the same general procedure as for the preparation of 6-ethynyl-3,4-dihydro-4,4-dimethyl-naphthalen-1(2H)-one (Compound K), 384 mg (1.25 mmol) of 7-bromo-3,4-dihydro-1(2H)-(3-pentylidene)-4,4-dimethylnaphthalene (Compound R) was converted into the title compound using 2.1 g (21 mmol) of trimethylsilylacetylene, 12 mg (0.06 mmol) of cuprous iodide, 43 mg (0.06 mmol) of bis(triphenylphosphine)palladium(II) chloride and 70 mg (0.5 mmol) of potassium carbonate.
PMR (CDCl.sub.3): .delta.1.04 (3H, t, J=7.6 Hz), 1.13 (3H, t, J=7.4 Hz), 1.23 (6H, s), 1.63 (2H, t, J=7.0 Hz), 2.21 (2H, q, J=7.4 Hz), 2.27 (2H, q, J=7.6 Hz), 2.48 (2H, t, J=7.0 Hz), 3.00 (1H, s), 7.23 (1H, d, J=8.0 Hz), 7.28 (1H, dd, J=2.3, 8.0 Hz), 7.35 (1H, d, J=2.3 Hz).
Ethyl-4-iodobenzoate
To a suspension of 10 g (40.32 mmol) of 4-iodobenzoic acid in 100 ml absolute ethanol was added 2 ml thionyl chloride and the mixture was then heated at reflux for 3 hours. Solvent was removed in vacuo and the residue was dissolved in 100 ml ether. The ether solution was washed with saturated NaHCO.sub.3 and saturated NaCl solutions and dried (MgSO.sub.4). Solvent was then removed in vacuo and the residue Kugelrohr distilled (100 degrees C; 0.55 mm) to give the title compound as a colorless oil, PMR (CDCl.sub.3): .delta.1.42 (3H, t, J.about.7 Hz), 4,4 (2H, q, J.about.7 Hz), 7.8 (4H).
Ethyl 6-chloronicotinate
A mixture of 15.75 g (0.1 mol) 6-chloronicotinic acid, 6.9 g (0.15 mol) ethanol, 22.7 g (0.11 mol) dicyclohexylcarbodiimide and 3.7 g dimethylaminopyridine in 200 ml methylene chloride was heated at reflux for 2 hours. The mixture was allowed to cool, solvent removed in vacuo and the residue subjected to flash chromatography to give the title compound as a low-melting white solid. PMR (CDCl.sub.3): .delta.1.44 (3H, t, J.about.6.2 Hz) 4.44 (2H, q, J.about.4.4 Hz), 7.44 (1H, d, J.about.8.1 Hz), 8.27 (1H, dd, J.about.8.1 Hz, 3 Hz), 9.02 (1H, d, J.about.3 Hz).
6-Iodonicotinic acid
To 27.97 g (186.6 mmol) of sodium iodide cooled to -78.degree. C. was added 121.77 g (71.6 ml, 952.0 mmol) of hydriodic acid (57 wt %). The reaction mixture was allowed to warm slightly with stirring for 5 minutes, and then 30.00 g (190.4 mmol) of 6-chloronicotinic acid was added. The resulting mixture was allowed to warm to room temperature with stirring and then heated at 120.degree.-125.degree. C. in an oil bath for 42 hours. A dark brown layer formed above the yellow solid material. The reaction mixture was allowed to cool to room temperature and then poured into acetone (chilled to 0.degree. C.). The resultant yellow solid was collected by filtration, washed with 200 ml of 1N NaHSO.sub.3 solution, and dried in high vacuum (3 mm Hg) to give the title compound as a pale yellow solid.
PMR (DMSO-d.sub.6): .delta.7.90 (1H, dd, J=8.1, 2 Hz), 7.99 (1H, d, J=8.1 Hz), 8.80 (1H, d, J=2.Hz).
Ethyl 6-iodonicotinate
To a suspension of 23.38 g (94.2 mmol) of 6-iodonicotinic acid in 100 ml of dichloromethane was added a solution of 19.86 g (103.6 mmol) of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride in 250 ml of dichloromethane. To this suspension was added 12.40 g (15.8 ml, 269.3 mmol) of ethanol (95%) and 1.15 g (9.4 mmol) of 4-dimethylaminopyridine. The resulting solution mixture was then heated at 50.degree. C. in an oil bath for 24.5 hours, concentrated in vacuo, partitioned between 200 ml of water and 250 ml of ethyl ether, and the layers were separated. The aqueous phase was washed with 2.times.150 ml-portions of ethyl ether. All organic phases were combined, washed once with 75 ml of brine solution, dried over MgSO.sub.4, filtered and concentrated in vacuo to a yellow solid. Purification by flash chromatography (silica, 10% ethyl acetate in hexane) yielded the title compound as a white solid.
PMR (CDCl.sub.3): .delta.1.41 (3H, t, J=7.1 Hz), 4.41 (2H, q, J=7.1 Hz), 7.85 (1H, d, J=8.2 Hz), 7.91 (1H, dd, J=8.2, 2.1 Hz), 8.94 (1H, d, J=2.1 Hz).
Ethyl 4, (5,6,7,8-tetrahydro-8,8-dimethyl-5-oxonaphth-2-yl)ethynyl!benzoate
(Compound 1)
To a solution of 8.8 g (47.8 mmol) of 6-ethynyl-1,2,3,4-tetrahydro-4,4-dimethylnaphthalen-1-one (Compound K) flushed for 15 minutes with a stream of argon, and 13.2 g (47.8 mmol) of ethyl 4-iodobenzoate in 200 ml of triethylamine was added 1.1 g (1.6 mmol) of bis(triphenylphosphine)palladium(II) chloride and 0.30 g (1.6 mmol) of cuprous iodide. The solution mixture was flushed with argon for 5 minutes and then stirred at ambient temperature for 18 hours. The reaction mixture was filtered through celite and the filtrate concentrated in vacuo. Purification by flash chromatography (silica, 10% EtOAc-hexane) yielded the title compound as a white solid.
PMR (CDCl.sub.3): .delta.1.41 (3H, t, J=7.2 Hz), 1.43 (6H, s), 2.04 (2H, t, J=7.0 Hz), 2.75 (2H, t, J=7.0 Hz), 4.40 (2H, q, J=7.2 Hz), 7.46 (1H, dd, J=1.5, 8.1 Hz), 7.60 (1H, d, J=1.5 Hz), 7.63 (2H, d, J=8.4 Hz), 8.01 (1H, d, J=8.1 Hz), 8.05 (2H, d, J=8.4 Hz).
Ethyl 4- (5,6,7,8-tetrahydro-8,8-dimethyl-5-oxonaphth-3-yl)ethynyl!benzoate
(Compound 2)
Employing the same general procedure as for the preparation of ethyl 4- (5,6,7,8-tetrahydro-8,8-dimethyl-5-oxonaphth-2-yl)ethynyl!benzoate (Compound 1), 4 g (21.7 mmol) of 7-ethynyl-3,4-dihydro-4,4-dimethylnaphthalen-1(2H)-one (Compound L) was converted into the title compound using 6 g (21.7 mmol) of ethyl 4-iodobenzoate, 5 g (7.2 mmol) of bis(triphenylphosphine)palladium(II) chloride and 1.4 g (7.2 mmol) of cuprous iodide.
PMR (CDCl.sub.3): .delta.1.41 (3H, t, J=7.2 Hz), 1.41 (6H, s), 2.04 (2H, t, J=6.5 Hz), 2.76 (2H, t, J=6.5 Hz), 4.40 (2H, q, J=7.2 Hz), 7.44 (1H, d, J=8.2 Hz), 7.59 (2H, d, J=8.4 Hz), 7.68 (1H, dd, J=1.8, 8.2 Hz), 8.04 (2H, d, J=8.4 Hz), 8.15 (1H, d, J=1.8 Hz).
4- (5,6,7,8-tetrahydro-8,8-dimethyl-5-oxonaphth-2-yl)ethynyl!benzoic acid
(Compound 7)
To a suspension of 0.30 g (0.87 mmol) of ethyl 4- (5,6,7,8-tetrahydro-8,8-dimethyl-5-oxonaphth-2yl)ethynyl!benzoate (Compound 1) in 4 ml of THF and 2 ml of ethanol was added 2 ml (2 mmol) of LiOH (1N aqueous solution). The reaction mixture was stirred at room temperature for 4 hours, concentrated in vacuo to near dryness, partitioned between EtOAc and 1 ml of water and acidified to pH 4 with 10% HCl. The aqueous layer was extracted with EtOAc and then the organic layer was dried over Na.sub.2 SO.sub.4 and concentrated in vacuo to give the title compound as a light yellow solid.
PMR (DMSO-d.sub.6): .delta.1.39 (6H, s), 1.98 (2H, t, J=7.0 Hz), 2.70 (2H, t, J=7.0 Hz), 7.54 (1H, dd, J=1.5, 8.1 Hz), 7.73 (2H, d, J=8.4 Hz), 7.77 (1H, d, J=1.5 Hz), 7.90 (1H, d, J=8.1 Hz), 8.00 (2H, d, J=8.4 Hz).
Ethyl 4- (5,6,7,8-tetrahydro-5-hydroxy-8,8-dimethyl-5-carboethoxymethylnaphth-2-yl)ethynyl!benzoate
(Compound 113)
To a refluxing solution of 1.00 g (15.30 mmol) of 20 mesh, granular zinc (activated prior to use by washing with 2% HCl, water, 95% ethanol, acetone, anhydrous Et.sub.2 O and then dried in vacuum for several hours) in 20 ml of dry benzene was slowly added a mixture of 0.23 ml (1.62 mmol) of ethyl bromoacetate, 0.28 g (0.81 mmol) of ethyl 4- (5,6,7,8-tetrahydro-8,8-dimethyl-5-oxonaphth-2-yl)ethynyl!benzoate (Compound 1) in 10 ml of dry benzene. The resulting mixture was refluxed for 2 hours, cooled to room temperature and the precipitate filtered through celite. The filtrate was washed with cold 15% H.sub.2 SO.sub.4, sat. aqueous NaHCO.sub.3 and brine. The organic phase was dried over Na.sub.2 SO.sub.4 and concentrated in vacuo to a yellow oil. Purification by flash chromatography (silica, 10% EtOAc-hexane) yielded the title compound as a light yellow solid.
PMR (CDCl.sub.3): .delta.1.30 (6H, s), 1.42 (3H, t, J=7.1 Hz), 1.75 (2H, m), 2.08 (2H, m), 2.77 (2H, s), 4.22 (3H, m), 4.39 (2H, q, J=7.1 Hz), 7.38 (1H, dd, J=1.7, 6.5 Hz), 7.49 (1H, d, J=1.6 Hz), 7.59 (3H, m), 8.02 (2H, d, J=8.4 Hz).
Ethyl 4- (7,8-dihydro-8,8-dimethyl-5-carboethoxymethylnaphth-2-yl)ethynyl!benzoate
(Compound 114)
and
Ethyl 4- (5-carboethoxymethylidene-7,8-dihydro-8,8-dimethylnaphth-2-yl)ethynyl!benzoate
(Compound 115)
To a solution of 0.50 g (1.15 mmol) of ethyl 4- (7,8-dihydro-5-hydroxy-8,8-dimethyl-5-carboethoxymethylnaphth-2-yl)ethynyl!benzoate (Compound 113) in 25 ml of dry benzene was added 2.12 g (8.90 mmol) of (methoxy carbonylsulfamoyl)triethyl ammonium hydroxide (Burgess Reagent). The reaction mixture was heated at 50.degree. C. for 30 minutes, cooled to room temperature and concentrated in vacuo. The residue was diluted with EtOAc, washed with water and brine, dried over Na.sub.2 SO.sub.4 and concentrated in vacuo to an oil. Purification by flash chromatography (silica, 25% EtOAc-hexane) yielded the title compounds as solids in a ratio of 5 to 1, respectively.
Ethyl 4- (7,8-dihydro-8,8-dimethyl-5-carboethoxymethylnaphth-2-yl)ethynyl!benzoate
(Compound 114)
PMR (CDCl.sub.3) .delta.1.17 (3H, t, J=2.8 Hz), 1.30 (6H, s), 1.41 (3H, t, J=7.1 Hz), 2.26 (2H, d, J=4.6 Hz), 3.46 (2H, s), 4.12 (2H, q, J=7.2 Hz), 4.38 (2H, q, J=7.1 Hz), 5.96 (1H, s), 7.17 (1H, d, J=8.0 Hz), 7.36 (1H, dd, J=1.7, 6.4 Hz), 7.48 (1H, d, J=1.7 Hz), 7.58 (2H, d, J=6.5 Hz), 8.01 (2H, d, J=6.5 Hz).
Ethyl 4- (5-carboethoxymethylidene-7,8-dihydro-8,8-dimethylnaphth-2-yl)ethynyl!benzoate
(Compound 115)
PMR (CDCl.sub.3) .delta.1.32 (6H, s), 1.41 (3H, t, J=7.1 Hz), 1.73 (3H, t, J=6.7 Hz), 3.22 (2H, m), 4.22 (2H, q, J=7.1 Hz), 4.39 (2H, q, J=7.1 Hz), 6.30 (1H, d, J=1.8 Hz), 7.35 (1H, dd, J=1.7, 6.6 Hz), 7.55 (1H, d, J=1.6 Hz), 7.60 (3H, dd, J=2.0, 6.4 Hz), 8.04 (2H, d, J=6.5 Hz).
(Trimethylsilyl)ethyl 4- (5,6,7,8-tetrahydro-8,8-dimethyl-5-oxonaphth-2-yl)ethynyl!benzoate
(Compound 116)
To a solution of 0.24 g (0.73 mmol) of 4- (5,6,7,8-tetrahydro-8,8-dimethyl-5-oxonaphth-2-yl)ethynyl!benzoic acid (Compound 7) in 10 ml of dry CH.sub.2 Cl.sub.2 was added 0.09 g (0.74 mmol) of dimethylaminopyridine, 0.115 ml (0.80 mmol) of trimethylsilylethanol and 0.17 g (0.88 mmol) of 1-(3-dimethylaminopropyl)-3-ethyl carbodiimide hydrochloride. The reaction mixture was stirred at 25.degree. C. for 5 hours, washed with sat. aqueous NaHCO.sub.3 and brine, dried over Na.sub.2 SO.sub.4 and concentrated in vacuo to an oil. Purification by flash chromatography (silica, 10% EtOAc-hexane) yielded the title compound as a white solid.
PMR (CDCl.sub.3) .delta.: 0.09 (9H, s), 1.14 (2H, m), 1.42 (6H, s), 2.03 (2H, t, J=7.1 Hz), 2.74 (2H, t, J=6.5 Hz), 4.43 (2H, t, J=8.5 Hz), 7.45 (1H, dd, J=1.5 , 6.7 Hz), 7.61 (3H, d, J=7.0 Hz), 8.03 (3H, t, J=6.7 Hz).
Trimethylsilylethyl 4- (5,6,7,8-tetrahydro-5-hydroxy-8,8-dimethyl-5-carboethoxymethylnaphth-2-yl)ethynyl!benzoate
(Compound 117)
Employing the same general procedure as for the preparation of ethyl 4- (5,6,7,8-tetrahydro-5-hydroxy-8,8-dimethyl-5-carboethoxymethylnaphth-2-yl)ethynyl!benzoate (Compound 113), 0.38 g (0.89 mmol) of trimethylsilylethyl 4- (5,6,7,8-tetrahydro-8,8-dimethyl-5-oxonaphth-2-yl)ethynyl!benzoate (Compound 116), was converted into the title compound (yellow solid) using 0.50 g (7.65 mmol) of zinc, 0.20 ml (1.78 mmol) of ethyl bromoacetate and 20 ml of dry benzene.
PMR (CDCl.sub.3) .delta.: 0.09 (9H, s), 1.14 (2H, m), 1.28 (8H, m), 1.74 (2H, m), 2.07 (2H, m), 2.77 (2H, s), 4.20 (3H, m), 4.42 (3H, t, J=8.4 Hz), 7.37 (1H, dd, J=1.6, 6.7 Hz), 7.49 (1H, s), 7.58 (3H, dd, J=3.8, 7.0 Hz), 8.01 (2H, d, J=8.4 Hz).
4- (5,6,7,8-tetrahydro-5-hydroxy-8,8-dimethyl-5-carboethoxymethylnaphth-2-yl)ethynyl!benzoic acid
(Compound 118)
To a solution of 0.25 9 (0.49 mmol) of trimethylsilylethyl 4- (5,6,7,8-tetrahydro-5-hydroxy-8,8-dimethyl-5-carboethoxy-methylnaphth-2-yl)ethynyl!benzoate (Compound 117) in 5 ml of dry THF (flushed with argon) was added 1.48 ml (1.5 mmol) of tetrabutyl ammonium fluoride (1M solution in THF). The reaction mixture was stirred at room temperature for 12 hours, concentrated in vacuo to an oil and slowly diluted with water. The solution was acidified to pH 4 with 10% HCl and extracted with Et.sub.2 O. The organic layer was dried over Na.sub.2 SO.sub.4, concentrated in vacuo to an oil and purified by flash chromatography (silica, 90% EtOAc-hexane) to give the title compound as a white solid.
PMR (CDCl.sub.3) .delta.: 1.30 (9H, m), 1.72 (2H, m), 2.08 (2H, m), 2.78 (2H, s), 4.21 (2H, q, J=7.0 Hz), 7.38 (1H, dd, J=1.5, 6.6 Hz), 7.50 (1H, s), 7.58 (1H, d, J=8.2 Hz), 7.62 (2H, d, J=8.4 Hz), 8.10 (2H, d, J=8.4 Hz).
Ethyl 4- (5-hydroxy-18-dimethyl-5-carboethoxymethyl-5,6,7,8-tetrahydronapth-3-yl)ethynyl!benzoate
(Compound 119)
Employing the same general procedure as for the preparation of ethyl 4- (7,8-dihydro-5-hydroxy-8,8-dimethyl-5-carboethoxymethylnaphth-2-yl)ethynyl!benzoate (Compound 113), 1.00 g (2.88 mmol) of ethyl 4- (5,6,7,8-tetrahydro-8,8-dimethyl-5-oxonaphth-3-yl)ethynyl!benzoate (Compound 2) was converted into the title compound (light yellow solid) using 1.00 g (15.30 mmol) of zinc, 0.639 ml (5.76 mmol) of ethyl bromoacetate and 70 ml of dry benzene.
PMR (CDCl.sub.3) .delta.: 1.30 (9H, m), 1.40 (3H, t, J=7.1 Hz), 1.75 (2H, m), 2.04 (2H, m), 2.80 (2H, d, J=3.1 Hz), 4.21 (2H, m), 4.38 (2H, q, J=7.2 Hz), 7.30 (1H, d, J=8.3 Hz), 7.41 (1H, dd, J=1.8, 6.4 Hz), 7.57 (2H, d, J=6.7 Hz), 7.81 (1H, d, J=1.8 Hz), 8.03 (2H, d, J=8.4 Hz).
Ethyl 4- (7,8-dihydro-8,8-dimethyl-5-carboethoxymethylnaphth-3-yl)ethynyl!benzoate
(Compound 120)
and
Ethyl 4- (5-carboethoxymethylidene-7,8-dihydro-8,8-dimethylnaphth-3-yl)ethynyl!benzoate
(Compound 121)
Employing the same general procedure as for the preparations of ethyl 4- (7,8-dihydro-8,8-dimethyl-5-carboethoxymethylnaphth-2-yl)ethynyl!benzoate (Compound 114) and ethyl 4- (5-carboethoxymethylidene-7,8-dihydro-8,8-dimethylnaphth-2-yl)ethynyl!benzoate (Compound 115), 0.57 g (1.31 mmol) of ethyl 4- (5,6,7,8-tetrahydro-5-hydroxy-8,8-dimethyl-5-carboethoxymethylnaphth-3-yl)ethynyl!benzoate (Compound 119) was converted into the title compounds (yellow solid and white solid, respectively) using 15 ml of dry benzene, 6 ml of dry THF and 2.42 g (10.1 mmol) of (methoxy carbonylsulfamoyl) triethyl ammonium hydroxide (Burgess Reagent).
Ethyl 4- (7,8-dihydro-8,8-dimethyl-5-carboethoxymethylnaphth-3-yl)ethynyl!benzoate
(Compound 120)
PMR (CDCl.sub.3) .delta.1.22 (3H, t, J=7.1 Hz), 1.29 (6H, s), 2.70 (2H, d, J=4.4 Hz), 3.49 (2H, s), 4.17 (2H, q, J=7.1 Hz), 4.00 (2H, q, J=7.1 Hz), 5.96 (1H, t, J=4.5 Hz), 7.35 (3H, m), 7.58 (2H, d, J=8.4 Hz), 8.02 (2H, d, J=7.9 Hz).
Ethyl 4- (5-carboethoxymethylidene-7,8-dihydro-8,8-dimethylnaphth-3-yl)ethynyl!benzoate
(Compound 121)
PMR (CDCl.sub.3) .delta.1.31 (9H, m), 1.74 (2H, q, J=6.7 Hz), 3.24 (2H, t, J=3.3 Hz), 4.22 (2H, q, J=7.1 Hz), 4.40 (2H, q, J=7.1 Hz), 6.33 (1H, s), 7.37 (1H, d, J=7.9 Hz), 7.50 (1H, d, J=8.3 Hz), 7.59 (2H, d, J=7.7 Hz), 7.79 (1H, s), 8.04 (2H, d, J=8.4 Hz).
Ethyl 4- (5(6H)-cyclohexylidene-7,8-dihydro-8,8-dimethylnaphth-2-yl)ethynyl!benzoate
(Compound 131)
Employing the same general procedure as for the preparation of ethyl 4- (5,6,7,8-tetrahydro-8,8-dimethyl-5-oxonaphth-2-yl)ethynyl!benzoate (Compound 1), 289 mg (1.22 mmol) of 6-ethynyl-1(2H)-cyclohexylidene-3,4-dihydro-4,4-dimethylnaphthalene (Compound U) was converted into the title compound using 337 mg (1.22 mmol) of ethyl 4-iodobenzoate, 77 mg (0.4 mmol) of cuprous iodide and 286 mg (0.41 mmol) of bis(triphenylphosphine)palladium(II) chloride.
PMR (CDCl.sub.3): .delta.1.28 (6H, s), 1.38 (3H, t, J=7.1 Hz), 1.54-1.65 (8H, br s), 2.32 (2H, br s), 2.46 (2H, t, J=6.4 Hz), 2.51 (2H, t, J=7.1 Hz), 4.37 (2H, q, J=7.1 Hz), 7.16 (1H, d, J=8.0 Hz), 7.29 (1H, dd, J=1.7, 8.0 Hz), 7.47 (1H, d, J=1.7 Hz), 7.57 (2H, d, J=8.3 Hz), 8.00 (2H, d, J=8.3 Hz).
Ethyl 4- (5(6H)-cyclohexylidene-7,8-dihydro-8,8-dimethylnaphth-3-yl)ethynyl!benzoate
(Compound 132)
Employing the same general procedure as for the preparation of ethyl 4- (5,6,7,8-tetrahydro-8,8-dimethyl-5-oxonaphth-2-yl)ethynyl!benzoate (Compound 1), 289 mg (1.22 mmol) of 7-ethynyl-1(2H)-cyclohexylidene-3,4-dihydro-4,4-dimethylnaphthalene (Compound V) was converted into the title compound using 337 mg (1.22 mmol) of ethyl 4-iodobenzoate, 77 mg (0.4 mmol) of cuprous iodide and 286 mg (0.41 mmol) of bis(triphenylphosphine)palladium(II) chloride.
PMR (CDCl.sub.3): .delta.1.27 (6H, s), 1.40 (3H, t, J=7.1 Hz), 1.52-1.69 (8H, m), 2.33 (2H, br s), 2.46 (2H, t, J=6.2 Hz), 2.53 (2H, t, J=7.4 Hz), 4.38 (2H, q, J=7.1 Hz), 7.27 (1H, d, J=8.5 Hz), 7.33 (2H, m), 7.56 (2H, d, J=8.4 Hz), 8.00 (2H, d, J=8.4 Hz).
Ethyl 4- (7,8-dihydro-8,8-dimethyl-5(6H)-(3-pentylidene)naphth-2-yl)ethynyl!benzoate
(Compound 133)
Employing the same general procedure as for the preparation of ethyl 4- (5,6,7,8-tetrahydro-8,8-dimethyl-5-oxonaphth-2-yl)ethynyl!benzoate (Compound 1), 143 mg (0.57 mmol) of 6-ethynyl-3,4-dihydro-1(2H)-(3-pentylidene)-4,4-dimethylnaphthalene (Compound W) was converted into the title compound using 142 mg (0.51 mmol) of ethyl 4-iodobenzoate, 36 mg (0.19 mmol) of cuprous iodide and 130 mg (0.19 mmol) of bis(triphenylphosphine)palladium(II) chloride.
PMR (CDCl.sub.3): .delta.1.05 (3H, t, J=7.4 Hz), 1.13 (3H, t, J=7.3 Hz), 1.27 (6H, s), 1.40 (3H, t, J=7.1 Hz), 1.65 (2H, t, J=6.9 Hz), 2.17-2.32 (4H, m), 2.51 (2H, t, J=6.9 Hz), 4.38 (2H, q, J=7.1 Hz), 7.21 (1H, d, J=8.0 Hz), 7.32 (1H, dd, J=1.7, 8.0 Hz), 7.47 (1H, d, J=1.7 Hz), 7.58 (2H, d, J=8.2 Hz), 8.02 (2H, d, J=8.2 Hz).
Ethyl 4- (7,8-dihydro-8,8-dimethyl-5(6H)-(3-pentylidene)-naphth-3-yl)ethynyl!benzoate
(Compound 134)
Employing the same general procedure as for the preparation of ethyl 4- (5,6,7,8-tetrahydro-8,8-dimethyl-5-oxonaphth-2-yl)ethynyl!benzoate (Compound 1), 235 mg (0.94 mmol) of 7-ethynyl-3,4-dihydro-1(2H)-(3-pentylidene)-4,4-dimethylnaphthalene (Compound X) was converted into the title compound using 236 mg (0.85 mmol) of ethyl 4-iodobenzoate, 57 mg (0.3 mmol) of cuprous iodide and 220 mg (0.31 mmol) of bis(triphenylphosphine)palladium(II) chloride.
PMR (CDCl.sub.3): .delta.1.07 (3H, t, J=7.5 Hz), 1.15 (3H, t, J=7.5 Hz), 1.25 (6H, s), 1.40 (3H, t, J=7.1 Hz), 1.65 (2H, t, J=7.0 Hz), 2.22 (2H, q, J=7.5 Hz), 2.31 (2H, q, J=7.5 Hz), 2.50 (2H, t, J=7.0 Hz), 4.38 (2H, q, J=7.1 Hz), 7.28 (1H, d, J=8.0 Hz), 7.35 (1H, dd, J=1.7, 8.0 Hz), 7.39 (1H, d, J=1.7 Hz), 7.56 (2H, d, J=8.5 Hz), 8.01 (2H, d, J=8.5 Hz).
4- (5(6H)-cyclohexylidene-7,8-dihydro-8,8-dimethylnaphth-2-yl)ethynyl!benzoic acid
(Compound 135)
Employing the same general procedure as for the preparation of 4- (5,6,7,8-tetrahydro-8,8-dimethyl-5-oxonaphth-2-yl)ethynyl!benzoic acid (Compound 7), 95 mg (0.23 mmol) of ethyl 4- (5(6H)-cyclohexylidene-7,8-dihydro-8,8-dimethylnaphth-2-yl)ethynyl!benzoate (Compound 131) was converted to the title compound using 1 ml (1 mmol) of LiOH (1M aqueous solution).
PMR (CDCl.sub.3): .delta.1.24 (6H, s), 1.50-1.61 (8H, br s), 2.30 (2H, br s), 2.39 (2H, br s), 2.47 (2H, br s), 7.16 (1H, d, J=8.1 Hz), 7.34 (1H, dd, J=1.7 , 8.1 Hz), 7.48 (1H, d, J=1.7 Hz), 7.65 (2H, d, J=8.4 Hz), 7.96 (2H, d, J=8.4 Hz).
4- (5(6H)-cyclohexylidene-7,8-dihydro-8,8-dimethylnaphth-3-yl)ethynyl!benzoic acid
(Compound 136)
Employing the same general procedure as for the preparation of 4- (5,6,7,8-tetrahydro-8,8-dimethyl-5-oxonaphth-2-yl)ethynyl!benzoic acid (Compound 7), 95 mg (0.23 mmol) of ethyl 4- (5(6H)-cyclohexylidene-7,8-dihydro-8,8-dimethylnaphth-3-yl)ethynyl!benzoate (Compound 132) was converted to the title compound using 1 ml (1 mmol) of LiOH (1M aqueous solution).
PMR (Acetone-d.sub.6): .delta.1.27 (6H, s), 1.50-1.70 (8H, m), 2.33 (2H, br s), 2.49 (2H, br s), 2.53 (2H, t, J=7.1 Hz), 7.29 (1H, d, J=8.4 Hz), 7.35 (2H, br s), 7.61 (2H, d, J=8.1 Hz), 8.08 (2H, d, J=8.1 Hz).
4- (7,8-dihydro-8,8-dimethyl-5(6H)-(3-pentylidene)naphth-2-yl)ethynyl!benzoic acid
(Compound 137)
Employing the same general procedure as for the preparation of 4- (5,6,7,8-tetrahydro-8,8-dimethyl-5-oxonaphth-2-yl)ethynyl!benzoic acid (Compound 7), 90 mg (0.23 mmol) of ethyl 4- (7,8-dihydro-8,8-dimethyl-5(6H)-(3-pentylidene)naphth-2-yl)ethynyl!benzoate (Compound 133) was converted to the title compound using 0.5 ml (0.5 mmol) of LiOH (1M aqueuos solution).
PMR (CDCl.sub.3): .delta.1.05 (3H, t, J=7.6 Hz), 1.34 (3H, t, J=7.4 Hz), 1.27 (6H, s), 1.67 (2H, t, J=7.1 Hz), 2.20-2.34 (4H, m), 2.54 (2H, t, J=7.1 Hz), 7.27 (1H, d, J=7.9 Hz), 7.36 (1H, dd, J=1.7, 7.9 Hz), 7.53 (1H, d, J=1.7 Hz), 7.66 (2H, d, J=8.4 Hz), 8.05 (2H, d, J=8.4 Hz).
4- (7,8-dihydro-8,8-dimethyl-5(6H)-1(3-pentylidene)naphth-3-yl)ethynyl!benzoic acid
(Compound 138)
Employing the same general procedure as for the preparation of 4- (5,6,7,8-tetrahydro-8,8-dimethyl-5-oxonaphth-2-yl)ethynyl!benzoic acid (Compound 7), 90 mg (0.23 mmol) of ethyl 4- (7,8-dihydro-8,8-dimethyl-5(6H)-(3-pentylidene)naphth-3-yl)ethynyl!benzoate (Compound 134) was converted to the title compound using 1 ml (1.0 mmol) of LiOH (1M aqueous solution).
PMR (CDCl.sub.3): .delta.1.05 (3H, t, J=7.6 Hz), 1.16 (3H, t, J=7.5 Hz), 1.25 (6H, s), 1.65 (2H, t, J=6.9 Hz), 2.22 (2H, q, J=7.5 Hz), 2.31 (2H, q, J=7.6 Hz), 2.50 (2H, t, J=6.9 Hz), 7.28 (1H, d, J=8.1 Hz), 7.36 (1H, dd, J=1.7, 8.1 Hz), 7.41 (1H, d, J=1.7 Hz), 7.61 (2H, d, J=8.5 Hz), 8.08 (2H, d, J=8.5 Hz).
Ethyl 4- 7,8-dihydro-8,8-dimethyl-5 (E)(6H)-cyanomethylidene)naphth-2-yl!ethynyl!benzoate
(Compound 145)
Ethyl 4- 7,8-dihydro-8,8-dimethyl-5 (Z)(6H)-cyanomethylidene)naphth-2-yl!ethynyl!benzoate
(Compound 146)
To a cold solution (0.degree. C.) of 442 mg (2.5 mmol) of diethyl cyanomethylphosphonate in 8 ml of THF was added 450 mg (2.25 mmol) of potassium his (trimethylsilyl)amide. The mixture was stirred for 15 minutes and then a solution of 366 mg (1.08 mmol) of ethyl-4- (5,6,7,8-tetrahydro-8,8-dimethyl-5-oxonaphth-2-yl)ethynyl!benzoate (Compound 1) in 7 ml of THF was added via syringe. The mixture was stirred for 8 hours, diluted with Et.sub.2 O (100 ml), washed with water (10 ml) and brine (10 ml), dried over MgSO.sub.4 and concentrated in vacuo. Purification by chromatography (silica, 5% EtOAc-hexane) gave a 6:1 ratio of E:Z isomers. Separation by normal phase HPLC (Partisil 10, 5% EtOAc-hexane) gave the title compounds (RT=64 minutes and 70 minutes), respectively.
Ethyl 4- 7,8-dihydro-8,8-dimethyl-5(E)(6H)(cyanomethylidene)naphth-2-yl!ethynyl!benzoate
(Compound 145)
PMR (CDCl.sub.3): (RT=64 minutes) .delta.1.35 (6H, s), 1.41 (3H, t, J=7.1 Hz), 1.82 (2H, t, J=6.4 Hz), 2.94 (2H, t, J=6.4 Hz), 4.39 (2H, q, J=7.1 Hz), 5.72 (1H, d, J=1.7 Hz), 7.36 (1H, dd, J=1.6, 8.3 Hz), 7.50 (1H, d, J=8.3 Hz), 7.58 (1H, d, J=1.6 Hz), 7.61 (2H, d, J=8.4 Hz), 8.05 (2H, d, J=8.4 Hz).
Ethyl 4- 7,8-dihydro-8,8-dimethyl-5 (Z)6(H)-(cyanomethylidene)naphth-2-yl!ethynyl!benzoate
(Compound 146)
PMR (CDCl.sub.3): (RT=70 minutes) .delta.1.35 (6H, s), 1.42 (3H, t, J=7.1 Hz), 1.84 (2H, t, J=6.5 Hz), 2.64 (2H, t, J=6.5 Hz), 4.40 (2H, q, J=7.1 Hz), 5.32 (1H, d, J=1.4 Hz), 7.43 (1H, dd, J=1.6, 8.2 Hz), 7.57 (1H, d, J=1.6 Hz), 7.62 (2H, d, J=8.4 Hz), 8.05 (2H, d, J=8.4 Hz), 8.18 (2H, d, J=8.2 Hz).

Claims

1. A compound of the formula ##STR11## wherein R.sub.1 is hydrogen or alkyl of 1 to 10 carbons;
R.sub.2 and R.sub.3 are hydrogen, or alkyl of 1 to 6 carbons and the substituted ethynyl group occupies either the 2 or the 3 position of the tetrahydronaphthalene nucleus;
m is an integer having the value of 0-3;
o is an integer having the value 0-4;
Y is a phenyl group, said phenyl being optionally substituted with one or two R.sub.2 groups;
A is (CH.sub.2).sub.n where n is 0-5, lower branched chain alkyl having 3-6 carbons, cycloalkyl having 3-6 carbons, alkenyl having 2-6 carbons and 1 or 2 double bonds, alkynyl having 2-6 carbons and 1 or 2 triple bonds;
B is hydrogen, COOH or a pharmaceutically acceptable salt thereof, COOR.sub.8, CONR.sub.9 R.sub.10, --CH.sub.2 OH, CH.sub.2 OR.sub.11, CH.sub.2 OCOR.sub.11, CHO, CH(OR.sub.12).sub.2, --COR.sub.7, CR.sub.7 (OR.sub.12).sub.2, or tri-lower alkylsilyl, where R.sub.7 is an alkyl, cycloalkyl or alkenyl group containing 1 to 5 carbons, R.sub.8 is an alkyl group of 1 to 10 carbons or (trimethylsilyl)alkyl where the alkyl group has 1 to 10 carbons, or a cycloalkyl group of 5 to 10 carbons, or R.sub.8 is phenyl or lower alkylphenyl, R.sub.9 and R.sub.10 independently are hydrogen, an alkyl group of 1 to 10 carbons, or a cycloalkyl group of 5-10 carbons, or phenyl or lower alkylphenyl, R.sub.11 is lower alkyl, phenyl or lower alkylphenyl, and R.sub.12 is lower alkyl,
R.sub.19 is independently hydrogen, alkyl of 1 to 10 carbons, fluoro-substituted alkyl of 1 to 10 carbons, alkenyl of 2 to 10 carbons and having 1 to 3 double bonds, alkynyl having 2 to 10 carbons and 1 to 3 triple bonds, carbocyclic aryl selected from the group consisting of phenyl, C.sub.1 -C.sub.10 -alkylphenyl, naphthyl, C.sub.1 -C.sub.10 -alkylnaphthyl, phenyl-C.sub.1 -C.sub.10 alkyl, naphthyl-C.sub.1 -C.sub.10 alkyl; CN, CHO, CH(OR.sub.12).sub.2, (CH.sub.2).sub.p CO.sub.2 R.sub.8 (CH.sub.2).sub.p CH.sub.2 OH, (CH.sub.2).sub.p CH.sub.2 OR.sub.11, (CH.sub.2).sub.p CH.sub.2 OCOR.sub.11, where p is an integer between 0 to 10, or the two R.sub.19 groups jointly represent 3 to 6 methylene groups which together with the alkylidene carbon complete a ring.
2. A compound of claim 1 where Y is phenyl.
3. A compound of claim 2 where the phenyl ring is 1,4 (para) substituted.
4. A compound of claim 1 where R.sub.2 is hydrogen.
5. A compound of claim 1 where R.sub.3 is hydrogen.
6. A compound of claim 1 where R.sub.19 is hydrogen, alkyl of 1 to 10 carbons, cyano (CN), COOR.sub.8, or the two R.sub.19 groups jointly form a --(CH.sub.2).sub.q radical where q is an integer having the values of 3 to 7.
7. A compound of the formula ##STR12## wherein R.sub.1 is hydrogen or alkyl of 1 to 10 carbons;
R.sub.2 and R.sub.3 are hydrogen, or alkyl of 1 to 6 carbons and the substituted ethynyl group occupies either the 2 or the 3 position of the tetrahydronaphthalene nucleus;
m is an integer having the value of 0-3;
o is an integer having the value 0-4;
Y is phenyl said phenyl being optionally substituted with one or two R.sub.2 groups;
A is (CH.sub.2).sub.n where n is 0-5, lower branched chain alkyl having 3-6 carbons, cycloalkyl having 3-6 carbons, alkenyl having 2-6 carbons and 1 or 2 double bonds, alkynyl having 2-6 carbons and 1 or 2 triple bonds;
B is hydrogen, COOH or a pharmaceutically acceptable salt thereof, COOR.sub.8, CONR.sub.9 R.sub.10, --CH.sub.2 OH, CH.sub.2 OR.sub.11, CH.sub.2 OCOR.sub.11, CHO, CH(OR.sub.12).sub.2, --COR.sub.7, CR.sub.7 (OR.sub.12).sub.2, or tri-lower alkylsilyl, where R.sub.7 is an alkyl, cycloalkyl or alkenyl group containing 1 to 5 carbons, R.sub.8 is an alkyl group of 1 to 10 carbons or (trimethylsilyl)alkyl where the alkyl group has 1 to 10 carbons, or a cycloalkyl group of 5 to 10 carbons, or R.sub.8 is phenyl or lower alkylphenyl, R.sub.9 and R.sub.10 independently are hydrogen, an alkyl group of 1 to 10 carbons, or a cycloalkyl group of 5-10 carbons, or phenyl or lower alkylphenyl, R.sub.11 is lower alkyl, phenyl or lower alkylphenyl, R.sub.12 is lower alkyl, and
R.sub.19 is independently hydrogen, alkyl of 1 to 10 carbons, CN, CH.sub.2 CO.sub.2 R.sub.8 or the two R.sub.19 groups jointly represent 3 to 6 methylene groups which together with the alkylidene carbon complete a ring.
8. A compound of claim 7 where A is (CH.sub.2).sub.n where n is 0-5 and where B is COOH or a pharmaceutically acceptable salt thereof, COOR.sub.8, or CONR.sub.9 R.sub.10.
9. A compound of the formula ##STR13## wherein X.sub.2 is CH;
R.sub.8 is hydrogen, an alkyl group of 1 to 10 carbons or (trimethylsilyl)alkyl where the alkyl group has 1 to 10 carbons;
R.sub.19' is H, cyano (CN) or lower alkyl, and
R.sub.19" is H, cyano (CN) or lower alkyl, or
the R.sub.19' and R.sub.19" groups jointly form a --(CH.sub.2).sub.5 -- group, and the substituted ethynyl group occupies the 2 or 3 position of the tetrahydronaphthalene nucleus.
10. A compound of claim 9 where the R.sub.19' and R.sub.19" groups jointly form a --(CH.sub.2).sub.5 -group.
11. A compound of claim 10 where R.sub.8 is H or C.sub.2 H.sub.5.
12. A compound of claim 9 where the R'.sub.19 is CO.sub.2 C.sub.2 H.sub.5 and R".sub.19 is H.
13. A compound of claim 12 where R.sub.8 is H or C.sub.2 H.sub.5.
14. A compound of claim 9 where the R.sub.19' is C.sub.2 H.sub.5 and R.sub.19" is C.sub.2 H.sub.5.
15. A compound of claim 14 where R.sub.8 is H or C.sub.2 H.sub.5.
16. A compound of claim 9 where one of the R.sub.19' and R.sub.19" groups is cyano (CN) and the other is H.
17. A compound of claim 16 where R.sub.8 is H or C.sub.2 H.sub.5.

US Referenced Citations (49)

Number	Name	Date
4096341	Frazer	Jun 1978
4326055	Loeliger	Apr 1982
4391731	Boller et al.	Jul 1983
4695649	Magami et al.	Sep 1987
4723028	Shudo	Feb 1988
4739098	Chandraratna	Apr 1988
4740519	Shroot et al.	Apr 1988
4810804	Chandraratna	Mar 1989
4826969	Maignan et al.	May 1989
4826984	Berlin et al.	May 1989
4855320	Chatterjee et al.	Aug 1989
4895868	Chandraratna	Jan 1990
4927947	Chandraratna	May 1990
4980369	Chandraratna	Dec 1990
4992468	Chandraratna	Feb 1991
5006550	Chandraratna	Apr 1991
5013744	Chandraratna	May 1991
5015658	Chandraratna	May 1991
5023341	Chandraratna	Jun 1991
5037825	Klaus et al.	Aug 1991
5045551	Chandraratna	Sep 1991
5053523	Chandraratna	Oct 1991
5068252	Chandraratna	Nov 1991
5089509	Chandraratna	Feb 1992
5130335	Chandraratna	Jul 1992
5134159	Chandraratna	Jul 1992
5162546	Chandraratna	Nov 1992
5175185	Chandraratna	Dec 1992
5183827	Chandraratna	Feb 1993
5202471	Chandraratna	Apr 1993
5231113	Chandraratna	Jul 1993
5234926	Chandraratna	Aug 1993
5248777	Chandraratna	Sep 1993
5264456	Chandraratna	Nov 1993
5264578	Chandraratna	Nov 1993
5272156	Chandraratna	Dec 1993
5278318	Chandraratna	Jan 1994
5324744	Chandraratna	Jun 1994
5324840	Chandraratna	Jun 1994
5326898	Chandraratna	Jul 1994
5344959	Chandraratna	Sep 1994
5346895	Chandraratna	Sep 1994
5346915	Chandraratna	Sep 1994
5348972	Chandraratna	Sep 1994
5348975	Chandraratna	Sep 1994
5349105	Chandraratna	Sep 1994
5354752	Chandraratna	Oct 1994
5380877	Chandraratna	Jan 1995
5391753	Chandraratna	Feb 1995

Foreign Referenced Citations (22)

Number	Date	Country
170105A		EPX
0098591	Jan 1984	EPX
01307795	Jan 1985	EPX
0176032	Apr 1986	EPX
176034A	Apr 1986	EPX
0176033	Apr 1986	EPX
0253302	Jan 1988	EPX
0272921	Jun 1988	EPX
0284288	Sep 1988	EPX
0303915	Feb 1989	EPX
0315071	May 1989	EPX
0350846	Jul 1989	EPX
3316932	Nov 1983	DEX
3524199	Jan 1986	DEX
3602473	Jul 1987	DEX
3708060	Sep 1987	DEX
3715955	Nov 1987	DEX
2190378	Nov 1987	GBX
WO8500806	Feb 1985	WOX
WO8504652	Oct 1985	WOX
WO9116051	Oct 1991	WOX
WO9206948	Apr 1992	WOX

Non-Patent Literature Citations (19)

Entry
A General Synthesis of Terminal and Internal Arylalkynes by the Palladium-Catalyzed Reaction of Alkynylzinc Reagents with Aryl Halides by Anthony O. King and Ei-ichi Negishi, J. Org. Chem. 43 No. 2, (1978) pp. 358-360.
Conversion of Methyl Ketones into Terminal Acetylenes and (E)-Tri-substituted Olefins of Terpenoid Origin by Ei-ichi, Anthony O. King and William L. Klima, J. Org. Chem. 45 No. 12, (1980) pp. 2526-2528.
Sporn et. al. in J. Amer. Acad. Derm. 15: pp. 756-764 (1986).
A Convenient Synthesis of Ethynylarenes and Diethylnylarenes by S. Takahashi, Y. Kuroyama, K. Sonogashira, N. Hagihara, Synthesis (1980) pp. 627-630.
Shudo et al. in Chem. Phar. Bull. 33 pp. 404-407, (1985).
Kagechika et. al. in J. Med. Chem. 31 pp. 2182-2192, (1988).
Chemistry and Biology of Synthetic Retinoids by Marcia I. Dawson and William H. Okamura, published by CRC Press Inc., (1990), pp. 334-335, 354.
Synthesis of 2,2'-Diacyl-1,1'-biaryls. Regiocontrolled Protection of . . . by Mervic, et al, J. Org. Chem., No. 45, pp. 4720-4725, (1980).
A Dopamine Receptor Model and Its Application in the Design of a New Class of Rigid Pyrrolo 2,3-g!isoquinoline Antipsychotics, Gary L. Olson, et al. American Chemical Society, (1981), Vo. 24, No. 9, pp. 1026-1031.
6.2.3 Conformational Restriction, Williams, et al., Drug Discovery and Development, (1987) The Humana Press, pp. 54-55.
V. Retinoid Structure-Biological Activity Relationships, Chemistry and Biology of Synthetic Retinoids, pp. 324-356, (1990).
Davis et al. J. Organomettalic Chem, 387 (1990) pp. 381-390.
Effects of 13-Cis-Retinoic Acid, All-Trans-Retinoic Acid, and Acitretin on the Proliferation, Lipid Synthesis and Keratin Expression of Cultured Human Sebocytes In Vitro, C. C. Zouboulis, The Journal of Investigative Dermatology, vol. 96, No. 5, (May 1991), pp. 792-797.
Organ maintenance of human sebaceous glands: in vitro effects of 13-cis retinoic acid and testosterone, John Ridden, et al., Journal of Cell Science, Vo. 95, (1990), pp. 125-136.
Characterization of Human Sebaceous Cells In Vitro, Thomas I. Doran, et al., The Journal of Investigative Dermatology, vol. 96, No. 3, Mar., 1991 pp. 341-348.
Synthesis and Evaluation of Stilbene and Dihydrostilbene Derivatives as Potential Anticancer Agents That Inhibit Tubulin Polymerization by Cushman, Mark et.al. J.Med. Chem (1991), 34, pp. 2579-2588.
Synthesis and Evaluation of New Protein-Tyrosine Kinase Inhibitors. Part 1. Pyridine-Containing Stilbenes and Amides by Cushman, Mark et al. Bioorganic & Medicinal Chemistry Letters, vol. 1, No.4, pp. 211-214, (1991).
Di-and Tri-methoxystyryl Derivatives of Heterocyclic Nitrogen Compounds by Bahner, C. T. et al. Arzneim-Forsch./Drug Res, 31 (I), Nr. 3 (1981), pp. 404-.
Retinobenzoic acids. 3. Structure-Activity Relationships of retinoidal Azobenzene-4-carboxylic acids and Stilbene-4-carboxylic acids by H. Kagechika et al., Journal of Medicinal Chemistry, (1989), 32, pp. 1098-1108.

Acetylenes disubstituted with a 5 substituted tetrahydronaphthyl group and with an aryl or heteroaryl group having retinoid-like biological activity

Information

Patent Number

Date Filed

Date Issued

Inventors

Original Assignees

Examiners

Agents

CPC

US Classifications

Field of Search

US

International Classifications

Abstract

Description

Claims

US Referenced Citations (49)

Foreign Referenced Citations (22)

Non-Patent Literature Citations (19)