Acquisition of an Analytical Ultracentrifuge

Information

• NSF Award
• 9216150

Owner

• UNIVERSITY OF MISSISSIPPI MEDICAL CENTER

• Award Id

  9216150

• Award Effective Date

  7/1/1993 - 31 years ago

• Award Expiration Date

  12/31/1995 - 29 years ago

• Award Amount

  $ 62,792.00

• Award Instrument

  Standard Grant

We propose to replace the Beckman Model E Analysis Ultracentrifuge with the new Beckman Optima XL-A Analytical Ultracentrifuge. This will maintain state-of-the-art instrumentation and facilitate physical biochemical analysis of numerous protein and nucleic acid systems currently under study in the laboratories of the proposed user group. Analytical ultracentrifugation is the best technique for studying associating macromolecular systems and is extremely useful for elucidating the interaction, size, and shape of small molecules, proteins and nucleic acids. Three primary and at least five secondary users will utilize the requested instrumentation in a variety of projects. Major projects include: 1) tubulin polymer formation and microtubule- associated protein self-association, 2) specificity of drug- nucleic acid interactions, 3) assembly of amino-tRNA syntheses complexes, 4) protein-RNA interactions and 5) secretory protein association. Minor projects include: 6) redox-electron carrier protein association, 7) molecular studies of secreted glycoproteins and 8) specificity of histone-DNA interactions. Acquisition of the requested equipment will have an immediated and significant impact on each of the projects listed in this proposal. The impact will include: 1) increased access to analytical ultracentrifugation for routine sedimentation velocity and equilibrium analysis, 2) access to rapid data acquisition and analysis through an interfaced PS/2 computer system, and 3) support for maintenance and repair of the ultracentrifuge. The analytical ultracentrifuge work is complemented by a variety of other biophysical approaches including circular dichroism, fluorescence spectroscopy, differential scanning calorimetry, stopped-flow kinetics, electron microscopy, and molecular biology, protein engineering. The applicants have an established record of shared instrumentation and of active collaboration in their research programs. The Optima XL-A will be only functioning analytical ultracentrifuge in the State of Mississippi.

• Program Officer

  Michael K. Lamvik

• Min Amd Letter Date

  6/18/1993 - 31 years ago

• Max Amd Letter Date

  6/18/1993 - 31 years ago

• ARRA Amount

• Name

  University of Mississippi Medical Center

• City

  Jackson

• State

  MS

• Country

  United States

• Address

  2500 North State Street

• Postal Code

  392164505

• Phone Number

  6018155000

• First Name

  Steven

• Last Name

  Case

• Start Date

  7/1/1993 12:00:00 AM

• First Name

  Mark

• Last Name

  Olson

• Email Address

  molson@biochem.umsmed.edu

• Start Date

  7/1/1993 12:00:00 AM

• First Name

  Jonathan

• Last Name

  Chaires

• Email Address

  j.chaires@louisville.edu

• Start Date

  7/1/1993 12:00:00 AM

• First Name

  John

• Last Name

  Correia

• Email Address

  jcorreia@biochem.umsmed.edu

• Start Date

  7/1/1993 12:00:00 AM

• First Name

  Mona

• Last Name

  Norcum

• Email Address

  mnorcum@biochem.umsmed.edu

• Start Date

  7/1/1993 12:00:00 AM

• Name

  Health

• Code

  203000

• Name

  Life Science Biological

• Code

  61

• Text

  INSTRUMENTAT & INSTRUMENT DEVP

• Code

  1108

• Text

  BIOTECHNOLOGY INSTRUMENTATION

• Code

  9126

• Text

  MULTI DIVISION/UNIVERSITY

• Code

  9162