Claims
- 1. A method of inhibiting attachment of substrate-dependent cells to a substrate comprising applying an adherent N,O-carboxymethylchitosan coating to said substrate such that attachment of substrate-dependent cells is inhibited.
- 2. The method of claim 1, wherein the substrate is selected from the group consisting of mammalian cells and tissue, non-mammalian cells and tissue, medical devices, fermentation units, bioreactors, and solid supports.
- 3. The method of claim 2, wherein the mammalian tissue is soft tissue.
- 4. The method of claim 2, wherein the mammalian tissue is hard tissue.
- 5. The method of claim 2, wherein said medical device is selected from the group of implantable and non-implantable devices consisting of stents, catheters, contact lenses, breast implants, pacemakers and shunts.
- 6. The method of claim 1, wherein the substrate-dependent cells are selected from the group consisting of fibroblasts, macrophages, epithelial cells, somatic cells and endothelial cells.
- 7. The method of claim 1, wherein said adherent N,O-carboxymethylchitosan is an adherent solution, hydrogel, paste, particle, bead, pellet, rehydratable film, or sponge.
- 8. The method of claim 1, wherein said adherent N,O-carboxymethylchitosan is covalently cross-linked.
- 9. The method of claim 1, wherein said adherent N,O-carboxymethylchitosan is ionically cross-linked.
- 10. A method of inhibiting attachment of a protein to a substrate comprising applying an adherent N,O-carboxymethylchitosan coating to said substrate such that attachment of said protein is inhibited.
- 11. The method of claim 10, wherein the substrate is selected from the group consisting of mammalian cells and tissue, non-mammalian cells and tissue, medical devices, fermentation units, bioreactors, and solid supports.
- 12. The method of claim 11, wherein the mammalian tissue is soft tissue.
- 13. The method of claim 11, wherein the mammalian tissue is hard tissue.
- 14. The method of claim 11, wherein said medical device is selected from the group consisting of stents, catheters, contact lenses, breast implants, pacemakers and shunts.
- 15. The method of claim 10, wherein said adherent N,O-carboxymethylchitosan is an adherent solution, hydrogel, paste, particle, bead, pellet, rehydratable film, or sponge.
- 16. The method of claim 10, wherein said adherent N,O-carboxymethylchitosan is covalently cross-linked.
- 17. The method of claim 10, wherein said adherent N,O-carboxymethylchitosan is ionically cross-linked.
- 18. A method of obtaining a population of cells comprising:
a) supplementing culture media with adherent N,O-carboxymethylchitosan; b) growing said population of cells in the supplemented media; and c) allowing said cells to grow or differentiate, wherein proliferation of substrate-dependent cells within said population is inhibited.
- 19. The method of claim 18, wherein said adherent N,O-carboxymethylchitosan is a solution, hydrogel or paste.
- 20. The method of claim 18, wherein said adherent N,O-carboxymethylchitosan hydrogel is covalently cross-linked.
- 21. The method of claim 18, wherein said adherent N,O-carboxymethylchitosan hydrogel is ionically cross-linked.
- 22. The method of claim 18, wherein substrate-dependent cell attachment within said population is inhibited.
- 23. A method of obtaining a population of cells suitable for use in protein or antibody production comprising:
a) supplementing culture media with adherent N,O-carboxymethylchitosan; and, b) growing said population of cells in the supplemented media, wherein intercellular attachment within said population is inhibited and production of said protein or antibody is enhanced.
- 24. The method of claim 23, wherein said adherent N,O-carboxymethylchitosan is a solution, hydrogel or paste.
- 25. The method of claim 23, wherein said adherent N,O-carboxymethylchitosan hydrogel is covalently cross-linked.
- 26. The method of claim 23, wherein said adherent N,O-carboxymethylchitosan hydrogel is ionically cross-linked.
- 27. The method of claim 23, wherein substrate-dependent cell attachment within said population is inhibited.
- 28. A method of inhibiting attachment of an inflammatory cell or protein to a medical device comprising coating said device with adherent N,O-carboxymethylchitosan such that inflammatory cell attachment is inhibited.
- 29. The method of claim 28, wherein said adherent N,O-carboxymethylchitosan is an adherent solution, hydrogel, paste, rehydratable film, or sponge.
- 30. The method of claim 28, wherein said adherent N,O-carboxymethylchitosan is covalently cross-linked.
- 31. The method of claim 28, wherein said adherent N,O-carboxymethylchitosan is ionically cross-linked.
- 32. The method of claim 28, wherein said medical device is selected from the group consisting of stents, catheters, pacemakers, breast implants, contact lenses and shunts.
- 33. The method of claim 28, wherein said inflammatory cell is selected from the group consisting of fibroblasts, macrophages, monocytes and lymphocytes.
- 34. A method of inhibiting substrate-dependent cell attachment in a cell-based product in contact with a solid support comprising contacting said cell based product with a solid support having an adherent NOCC coating.
- 35. The method of claim 34, wherein said adherent N,O-carboxymethylchitosan is an adherent solution, hydrogel, paste, rehydratable film, or sponge.
- 36. The method of claim 34, wherein said adherent N,O-carboxymethylchitosan is covalently cross-linked.
- 37. The method of claim 34, wherein said adherent N,O-carboxymethylchitosan is ionically cross-linked.
- 38. The method of claim 34, wherein said adherent N,O-carboxymethylchitosan is coated on said solid support.
- 39. The method of claim 34, wherein said cell-based product is a cell culture.
- 40. The method of claim 34, wherein said cell-based product is a biological sample.
- 41. The method of claim 40, wherein said biological sample is blood.
- 42. The method of claim 40, wherein said biological sample is plasma.
REFERENCE TO RELATED APPLICATIONS
[0001] This application is a continuation-in-part of U.S. patent application Ser. No. 09/315,480, entitled “ADHESIVE N,O-CARBOXYMETHYLCHITOSAN COATINGS WHICH INHIBIT ATTACHMENT OF SUBSTRATE-DEPENDENT CELLS AND PROTEINS,” filed May 20, 1999, the disclosure of which is incorporated herein by reference.
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
09315480 |
May 1999 |
US |
Child |
10672072 |
Sep 2003 |
US |