Ading color to cancer, adenovirus and flow cytometry to identify and capture CTCs

Information

  • Research Project
  • 8336845
  • ApplicationId
    8336845
  • Core Project Number
    R43CA163436
  • Full Project Number
    5R43CA163436-02
  • Serial Number
    163436
  • FOA Number
    RFA-CA-10-013
  • Sub Project Id
  • Project Start Date
    9/21/2011 - 13 years ago
  • Project End Date
    8/31/2013 - 11 years ago
  • Program Officer Name
    RAHBAR, AMIR M.
  • Budget Start Date
    9/13/2012 - 12 years ago
  • Budget End Date
    8/31/2013 - 11 years ago
  • Fiscal Year
    2012
  • Support Year
    02
  • Suffix
  • Award Notice Date
    9/13/2012 - 12 years ago

Ading color to cancer, adenovirus and flow cytometry to identify and capture CTCs

Ad'ing color to cancer: adenovirus & flow cytometry to identify & capture CTCs NanoSort RESEARCH & RELATED Other Project Information 7. PROJECT SUMMARY We propose a novel technique to identify and capture circulating tumor cels (CTCs) using enginered adenoviruses and sophisticated flow cytometry. Current techniques for detection of CTCs include reverse transcriptase-polymerase chain reaction (RT-PCR), flow cytometry, fluorescence in situ hybridization, and, more recently, microfluidics. Unfortunately, RT-PCR does not distinguish between viable metastatic CTC versus nucleic acids or celular fragments originating from the primary tumor. ! Antibody-based techniques cannot be used for detection of all cancers, but only those cancers that express the most common and well- characterized markers. As such, there is a desperate need to develop new diagnostic agents and tools that not only detect and capture CTCs but also quantify their malignant potential and identify 'up-front' the therapies that are most effective in ablating an individual patient's tumor. Despite the complexity and variability of cancers at a genome scale, a unifying theme is their growth deregulation phenotypes, the so-called hallmarks of cancer, which are conferred by mutations in a relatively small number of key pathways. Rather than focus on detecting individual genetic lesions that are numerous and highly variable between tumors, we propose to create diagnostic viruses that incorporate multiple transcriptional and molecular modules in their genomes to infect and detect a patient's tumor, report its molecular 'hallmarks' and its response to different therapies 'up- front'. Using these agents, the molecular lesions and malignant characteristics of any given tumor wil be rapidly discerned (within 24 hours) and scored via a standardized automated platform. Furthermore, these agents could also be used as reporters to determine rapidly and directly if a patient's tumor is likely to respond to a particular therapy. Our goal is to develop a standardized automated platform that provides point-of-care diagnostics to inform clinical decisions at a level of molecular sophistication and prognostic power that is not possible with any other detection system, biomarkers or correlative gene expression signatures. To achieve this, we will combine transformative new technological platforms developed at the Salk, UCSD, and NanoSort that label tumor cells in different colors based on their acquisition of molecular lesions that dictate malignant progression and response to therapy, facilitating their detection, quantification and isolation using an integrated 'lab-on a chip' flow cytometer. !

IC Name
NATIONAL CANCER INSTITUTE
  • Activity
    R43
  • Administering IC
    CA
  • Application Type
    5
  • Direct Cost Amount
  • Indirect Cost Amount
  • Total Cost
    152152
  • Sub Project Total Cost
  • ARRA Funded
    False
  • CFDA Code
    394
  • Ed Inst. Type
  • Funding ICs
    NCI:152152\
  • Funding Mechanism
    SBIR-STTR RPGs
  • Study Section
    ZCA1
  • Study Section Name
    Special Emphasis Panel
  • Organization Name
    NANOCELLECT BIOMEDICAL, INC.
  • Organization Department
  • Organization DUNS
    832751098
  • Organization City
    SAN DIEGO
  • Organization State
    CA
  • Organization Country
    UNITED STATES
  • Organization Zip Code
    921221937
  • Organization District
    UNITED STATES