Claims
- 1. A composition for the deswelling of corneas in organ culture, comprising a culture medium and a deswelling substance, wherein the deswelling substance is hydroxyethylstarch with an average molecular weight M.sub.w of from 70,000 to 200,000, with a degree of substitution MS of from 0.15 to 0.5, with a degree of substituting DS of from 0.15 to 0.5 and with a ratio of substitution at C2 to substitution at C6 of the anhydroglucose units of .gtoreq.8.
- 2. A composition as claimed in claim 1, wherein the average molecular weight M.sub.w is from 100,000 to 160,000, the degree of substitution MS is from 0.2 to 0.45, the degree of substitution DS is from 0.2 to 0.4 and the ratio of substitution at C2 to substitution at C6 of the anhydroglucose units is from 8 to 20.
- 3. A composition as claimed in claim 2, wherein the average molecular weight M.sub.w is 130,000.+-.20,000, the degree of substitution MS is from 0.38 to 0.45, the degree of substitution DS is from 0.32 to 0.40 and the ratio of substitution at C2 to substitution at C6 of the anhydroglucose units is from 8 to 20.
- 4. A composition as claimed in claim 1, which is used in the organ culture of human corneas.
- 5. A composition as claimed in claim 1, wherein the hydroxyethylstarch is used in a concentration of from 1 to 20% (weight/volume).
- 6. A composition as claimed in claim 5, wherein the concentration is from 2 to 15% (weight/volume).
- 7. A composition as claimed in claim 6, wherein the concentration is from 5 to 10% (weight/volume).
- 8. A composition as claimed in claim 7, wherein the concentration is 7.5% (weight/volume).
- 9. A method of deswelling corneas comprising contacting the corneas with the composition of claim 1.
Priority Claims (1)
Number |
Date |
Country |
Kind |
197 02 210 |
Jan 1997 |
DEX |
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RELATED APPLICATION
This application is a continuation of International Application Number PCT/EP98/00324, filed Jan. 22, 1998, which in turn claims priorty from erman Patent Application No. 197 02 210.3, filed Jan. 23,1997, both of which are incorporated herein by reference.
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Apr 1994 |
|
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DEX |
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DEX |
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Entry |
Walkenbach et al., Current Eye Research, vol. 10, 12, pp 1129-1136. The effects of UW solution and its components on corneal thickness during and after storage, 1991. |
Continuations (1)
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Number |
Date |
Country |
Parent |
PCTEP9800324 |
Jan 1998 |
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