Aggrecanase molecules

FIELD OF INVENTION

[0002] The present invention relates to the discovery of nucleotide sequences encoding novel aggrecanase molecules, the aggrecanase proteins and processes for producing them. The invention further relates to the development of inhibitors of, as well as antibodies to, the aggrecanase enzymes. These inhibitors and antibodies may be useful for the treatment of various aggrecanase-associated conditions including osteoarthritis.

BACKGROUND OF THE INVENTION

[0003] Aggrecan is a major extracellular component of articular cartilage. It is a proteoglycan responsible for providing cartilage with its mechanical properties of compressibility and elasticity. The loss of aggrecan has been implicated in the degradation of articular cartilage in arthritic diseases. Osteoarthritis is a debilitating disease which affects at least 30 million Americans (MacLean et al., J Rheumatol 25:2213-8 (1998)). Osteoarthritis can severely reduce quality of life due to degradation of articular cartilage and the resulting chronic pain. An early and important characteristic of the osteoarthritic process is loss of aggrecan from the HENDERSON extracellular matrix (Brandt and Mankin, “Pathogenesis of Osteoarthritis,” Textbook of Rheumatology, W B Saunders Company, Philadelphia, Pa., at 1355-1373 (1993)). The large, sugar-containing portion of aggrecan is thereby lost from the extra-cellular matrix, resulting in deficiencies in the biomechanical characteristics of the cartilage.

[0004] A proteolytic activity termed “aggrecanase” is thought to be responsible for the cleavage of aggrecan thereby having a role in cartilage degradation associated with osteoarthritis and inflammatory joint disease. Work has been conducted to identify the enzyme responsible for the degradation of aggrecan in human osteoarthritic cartilage. Two enzymatic cleavage sites have been identified within the interglobular domain of aggrecan. One (Asn34-Phe342) is observed to be cleaved by several known metalloproteases. Flannery et al., J Biol Chem, 267:1008-14 (1992); Fosang et al., Biochemical J., 304:347-351 (1994). The aggrecan fragment found in human synovial fluid, and generated by IL-1 induced cartilage aggrecan cleavage is at the Glu373-Ala374 bond (Sandy, et al., J Clin Invest, 69:1512-1516 (1992); Lohmander et al., Arthritis Rheum 36:1214-1222 (1993); Sandy et al., J Biol Chem., 266:8683-8685 (1991)), indicating that none of the known enzymes are responsible for aggrecan cleavage in vivo.

[0005] Recently, identification of two enzymes, aggrecanase-1 (ADAMTS 4) and aggrecanase-2 (ADAMTS-11) within the “Disintegrin-like and Metalloprotease with Thrombospondin type 1 motif” (ADAM-TS) family have been identified which are synthesized by IL-1 stimulated cartilage and cleave aggrecan at the appropriate site (Tortorella, et al., Science, 284:1664-6 (1999); Abbaszade et al., J Biol Chem, 274: 23443-23450 (1999)). It is possible that these enzymes could be synthesized by osteoarthritic human articular cartilage. It is also contemplated that there are other, related enzymes in the ADAM-TS family which are capable of cleaving aggrecan at the Glu373-Ala374 bond and could contribute to aggrecan cleavage in osteoarthritis. There is a need to identify other aggrecanase enzymes and determine ways to block their activity.

SUMMARY OF THE INVENTION

[0006] The present invention is directed to the identification of aggrecanase protein molecules capable of cleaving aggrecan, the nucleotide sequences which encode the aggrecanase enzymes, and processes for the production of aggrecanases. These enzymes are contemplated to be characterized as having proteolytic aggrecanase activity. The invention further includes compositions comprising these enzymes.

[0007] The invention also includes antibodies to these enzymes, in one embodiment, for example, antibodies that block aggrecanase activity. In addition, the invention includes methods for developing inhibitors of aggrecanase which block the enzyme's proteolytic activity. These inhibitors and antibodies may be used in various assays and therapies for treatment of conditions characterized by the degradation of articular cartilage.

[0008] The invention provides an isolated DNA molecule comprising a DNA sequence chosen from: the sequence of SEQ ID NO: 1 from nucleotide #1-#4080; SEQ ID NO: 3 from nucleotide #1-#3691; and naturally occurring human allelic sequences and equivalent degenerative codon sequences.

[0009] The invention also comprises a purified aggrecanase protein comprising an amino acid sequence chosen from: the amino acid sequence set forth in SEQ ID NO: 5 from amino acid #1-#1360; SEQ ID NO: 4 from amino acid #1-#1223; and homologous aggrecanase proteins consisting of addition, substitution, and deletion mutants of the sequences.

[0010] The invention also provides a method of producing a purified aggrecanase protein.

[0011] The human aggrecanase protein or a fragment thereof may be produced by the steps of culturing a host cell transformed with a DNA molecule according to the invention, and recovering and purifying from the culture medium a protein comprising the amino acid sequence set forth in SEQ ID NOS: 4 or 5.

[0012] The invention also provides an antibody that binds to a purified aggrecanase protein of the invention. It also provides a method for developing inhibitors of aggrecanase comprising the use of an aggrecanase protein chosen from SEQ ID NOS: 4, 5, and a fragment thereof.

[0013] Additionally, it provides a pharmaceutical composition for inhibiting the proteolytic activity of aggrecanase, wherein the composition comprises at least one antibody according to the invention and at least one pharmaceutical carrier. It also provides a method for inhibiting aggrecanase in a mammal comprising administering to said mammal an effective amount of the pharmaceutical composition and allowing the composition to inhibit aggrecanase activity.

BRIEF DESCRIPTION OF THE SEQUENCES

[0014] This table summarizes information on the sequence listings provided in this application

1SEQUENCESDESCRIPTION1Genscan predicted EST-162a.a. seq. of Genscan predicted EST-163full-length confirmed EST164a.a. seq. of SEQ ID NO. 35a.a. seq. of Genscan 2 nucleotidesequence6synthetic EST-16 Genscan predictedsequence7sa. seq. - zinc binding signature region8nucleotide primer9nucleotide primer10nucleotide primer11nucleotide primer12nucleotide primer13nucleotide primer14nucleotide primer15nucleotide primer16nucleotide primer17nucleotide primer18synthetic oligonucleotide19synthetic oligonucleotide20synthetic oligonucleotide21synthetic EST-16 predicted nucleotidessequencea.a. = amino acid

DETAILED DESCRIPTION OF THE INVENTION

[0015] I. Novel Aggrecanase Proteins

[0016] In one embodiment, the nucleotide sequence of an aggrecanase molecule of the present invention is chosen from SEQ ID NO: 1 from nucleotides #1-#4080, and SEQ ID NO: 3 from nucleotides #1-#3691. The invention further includes equivalent degenerative codon sequences of the sequences set forth in SEQ ID NOS: 1 and 3 as well as fragments thereof which exhibit aggrecanase activity. The full length sequence of the aggrecanase of the present invention may be obtained using the sequences of SEQ ID NOS: 1 or 3 to design probes for screening for the full sequence using standard techniques.

[0017] The amino acid sequence of the isolated aggrecanase-like molecule is set forth in SEQ ID NO: 5, as amino acids #1-#1360, and SEQ ID NO: 4, as amino acids #1-#1223. The invention further includes fragments of the amino acid sequences which encode molecules exhibiting aggrecanase activity.

[0018] The invention includes methods for obtaining the full length aggrecanase molecule, the DNA sequence obtained by this method and the protein encoded thereby. The method for isolation of the full length sequence involves utilizing the aggrecanase sequence set forth in SEQ ID NO: 1 or SEQ ID NO: 3 to design probes for screening, or otherwise screen, using standard procedures known to those skilled in the art.

[0019] The aggrecanase protein or a fragment thereof may be produced by culturing a cell transformed with the DNA sequence of SEQ ID NO: 1 comprising nucleotide #1-#4080 or SEQ ID NO: 3 comprising nucleotide #1-#3691 and recovering and purifying from the culture medium a protein characterized by the amino acid sequence set forth in SEQ ID NOS: 4 or 5. The purified expressed protein is substantially free from other proteinaceous materials with which it is co-produced, as well as from other contaminants. For production in mammalian cells, the DNA sequence further comprises a DNA sequence encoding a suitable propeptide 5′ to and linked in frame to the nucleotide sequence encoding the aggrecanase enzyme.

[0020] The human aggrecanase proteins produced by the method discussed above are characterized by having the ability to cleave aggrecan and having an amino acid sequence chosen from SEQ ID NOS: 4 or 5 variants of the amino acid sequence of SEQ ID NOS: 4 or 5 including naturally occurring allelic variants, and other variants in which the proteins retain the ability to cleave aggrecan characteristic of aggrecanase proteins. Preferred proteins include a protein which is at least about 80% homologous, and more preferably at least about 90% homologous, to the amino acid sequence shown in SEQ ID NOS: 4 or 5. Finally, allelic or other variations of the sequences of SEQ ID NOS: 4 or 5 whether such amino acid changes are induced by mutagenesis, chemical alteration, or by alteration of DNA sequence used to produce the protein, where the peptide sequence still has aggrecanase activity, are also included in the present invention. The present invention also includes fragments of the amino acid sequence of SEQ ID NOS: 4 or 5 which retain the activity of aggrecanase protein.

[0021] II. Identification of Homologous Aggrecanase Proteins and DNA Encoding Them

[0022] It is expected that additional human sequences and other species have DNA sequences homologous to human aggrecanase enzyme. The invention, therefore, includes methods for obtaining the DNA sequences encoding other aggrecanase proteins, the DNA sequences obtained by those methods, and the protein encoded by those DNA sequences. This method entails utilizing the nucleotide sequence of the invention or portions thereof to design probes to screen libraries for the corresponding gene from other species or coding sequences or fragments thereof from using standard techniques. Thus, the present invention may include DNA sequences from other species, which are homologous to the human aggrecanase protein and can be obtained using the human sequence. The present invention may also include functional fragments of the aggrecanase protein, and DNA sequences encoding such functional fragments, as well as functional fragments of other related proteins. The ability of such a fragment to function is determinable by assay of the protein in the biological assays described for the assay of the aggrecanase protein.

[0023] For example, SEQ ID NO. 3 was used in a query against the Genbank databases in a BLASTN 2.2.2 search. Several sequences were identified as similar to SEQ ID NO. 3, differing only by splicing, truncation, or incomplete sequence. These sequences are disclosed in the following patents: WO 02/24927, WO 02/16564, WO 01/83782, WO 01/98468, WO 02/10216, WO 01/59133, and WO 200175067-A2. Some accession numbers with homology to EST-16 include BF906533, AJ403134, BF906535, BF823025, and BF906528. It is believed that these sequences are all part of the same family of ADAMTS. The cloning of ADAMTS17 has been described in Cal, S., et al., Gene, 283 (1-2):49-62 (2002). See also, Colige et al., Cloning and Characterization of ADAMTS-14, A Novel ADAMTS Displaying High Homology with ADAMTS-2 and ADAMTS-3, J. Biol. Chem., 277(8):5756-5766 (2002).

[0024] Some examples of homologous, non-human sequences include a mouse sequence (sp:P97857), a rat sequence (sp:Q9WUQ1), Arabidopsis thaliana (NP 193602.1), Caenorhabditis elegans (NP 501792.1), and Drosophila melanogaster (NP 524645.1). It is expected that these sequences, from non-human species, are homologous to human aggrecanase enzymes.

[0025] The aggrecanase proteins provided herein also include factors encoded by the sequences similar to those of SEQ ID NO: I or SEQ ID NO: 3, but into which modifications or deletions are naturally provided (e.g., allelic variations in the nucleotide sequence which may result in amino acid changes in the protein) or deliberately engineered. For example, synthetic proteins may wholly or partially duplicate continuous sequences of the amino acid residues of SEQ ID NOS: 4 or 5. These sequences, by virtue of sharing primary, secondary, or tertiary structural and conformational characteristics with aggrecanase proteins may possess biological properties in common therewith. It is known, for example that numerous conservative amino acid substitutions are possible without significantly modifying the structure and conformation of a protein, thus maintaining the biological properties as well. For example, it is recognized that conservative amino acid substitutions may be made among amino acids with basic side chains, such as lysine (Lys or K), arginine (Arg or R) and histidine (His or H); amino acids with acidic side chains, such as aspartic acid (Asp or D) and glutamic acid (Glu or E); amino acids with uncharged polar side chains, such as asparagine (Asn or N), glutamine (Gln or Q), serine (Ser or S), threonine (Thr or T), and tyrosine (Tyr or Y); and amino acids with nonpolar side chains, such as alanine (Ala or A), glycine (Gly or G), valine (Val or V), leucine (Leu or L), isoleucine (Ile or I), proline (Pro or P), phenylalanine (Phe or F), methionine (Met or M), tryptophan (Trp or W) and cysteine (Cys or C). Thus, these modifications and deletions of the native aggrecanase may be employed as biologically active substitutes for naturally-occurring aggrecanase and in the development of inhibitors or other proteins in therapeutic processes. It can be readily determined whether a given variant of aggrecanase maintains the biological activity of aggrecanase by subjecting both aggrecanase and the variant of aggrecanase, as well as inhibitors thereof, to the assays described in the examples.

[0026] Other specific mutations of the sequences of aggrecanase proteins described herein involve modifications of glycosylation sites. These modifications may involve O-linked or N-linked glycosylation sites. For instance, the absence of glycosylation or only partial glycosylation results from amino acid substitution or deletion at asparagine-linked glycosylation recognition sites. The asparagine-linked glycosylation recognition sites comprise tripeptide sequences which are specifically recognized by appropriate cellular glycosylation enzymes. These tripeptide sequences are either asparagine-X-threonine or asparagine-X-serine, where X is usually any amino acid. A variety of amino acid substitutions or deletions at one or both of the first or third amino acid positions of a glycosylation recognition site (and/or amino acid deletion at the second position) results in non-glycosylation at the modified tripeptide sequence. Additionally, bacterial expression of aggrecanase-related protein will also result in production of a non-glycosylated protein, even if the glycosylation sites are left unmodified.

[0027] III. Novel Aggrecanase Nucleotide Sequences

[0028] Still a further aspect of the invention are DNA sequences coding for expression of an aggrecanase protein having aggrecanase proteolytic activity or other disclosed activities of aggrecanase. Such sequences include the sequence of nucleotide in a 5′ to 3′ direction set forth in SEQ ID NO: 1 or SEQ ID NO: 3 and DNA sequences which, but for the degeneracy of the genetic code, are identical to the DNA sequence of SEQ ID NO: 1 or SEQ ID NO: 3, and encode an aggrecanase protein.

[0029] Further included in the present invention are DNA sequences which hybridize under stringent conditions with the DNA sequence of SEQ ID NO: 1 or SEQ ID NO: 3 and encode a protein having the ability to cleave aggrecan. Preferred DNA sequences include those which hybridize under stringent conditions (see, T. Maniatis et al., Molecular Cloning (A Laboratory Manual), Cold Spring Harbor Laboratory at 387-389 (1982). Such stringent conditions comprise, for example, 0.1×SSC, 0.1% SDS, at 65° C.

[0030] Similarly, DNA sequences which code for aggrecanase proteins coded for by the sequences of SEQ ID NO: 1 or SEQ ID NO: 3, or aggrecanase proteins which comprise the amino acid sequences of SEQ ID NO: 4 or 5, but which differ in codon sequence due to the degeneracies of the genetic code or allelic variations (naturally-occurring base changes in the species population which may or may not result in an amino acid change) also encode the novel factors described herein. Variations in the DNA sequences of SEQ ID NO: 1 or SEQ ID NO: 3 which are caused by point mutations or by induced modifications (including insertion, HENDERSON deletion, and substitution) to enhance the activity, half-life or production of the proteins encoded are also encompassed in the invention.

[0031] The DNA sequences of the present invention are useful, for example, as probes for the detection of mRNA encoding aggrecanase in a given cell population. Thus, the present invention includes methods of detecting or diagnosing genetic disorders involving the aggrecanase, or disorders involving cellular, organ or tissue disorders in which aggrecanase is irregularly transcribed or expressed. Antisense DNA sequences may also be useful for preparing vectors for gene therapy applications. Antisense DNA sequences are also useful for in vivo methods, such as to introduce the antisense DNA into the cell, to study the interaction of the antisense DNA with the native sequences, and to test the capacity of a promoter operatively linked to the antisense DNA in a vector by studying the interaction of antisense DNA in the cell as a measure of how much antisense DNA was produced.

[0032] A further aspect of the invention includes vectors comprising a DNA sequence as described above in operative association with an expression control sequence therefor. These vectors may be employed in a novel process for producing an aggrecanase protein of the invention in which a cell line transformed with a DNA sequence encoding an aggrecanase protein in operative association with an expression control sequence therefor, is cultured in a suitable culture medium and an aggrecanase protein is recovered and purified therefrom. This process may employ a number of known cells both prokaryotic and eukaryotic as host cells for expression of the polypeptide. The vectors may be used in gene therapy applications. In such use, the vectors may be transfected into the cells of a patient ex vivo, and the cells may be reintroduced into a patient. Alternatively, the vectors may be introduced into a patient in vivo through targeted transfection.

[0033] The construction of vectors may involve modification of the aggrecanase-related DNA sequences. For instance, aggrecanase cDNA can be modified by removing the non-coding nucleotide on the 5′ and 3′ ends of the coding region. The deleted non-coding nucleotide may or may not be replaced by other sequences known to be beneficial for expression. These vectors are transformed into appropriate host cells for expression of aggrecanase-related proteins. Additionally, the sequence of SEQ ID NO: 1, SEQ ID NO: 3 or other sequences encoding aggrecanase-related proteins can be manipulated to express a mature aggrecanase-related protein by deleting aggrecanase encoding propeptide sequences and replacing them with sequences encoding the complete propeptides of other aggrecanase proteins.

[0034] One skilled in the art can manipulate the sequences of SEQ ID NO: 1 or SEQ ID NO: 3 by eliminating or replacing the mammalian regulatory sequences flanking the coding sequence with bacterial sequences to create bacterial vectors for intracellular or extracellular expression by bacterial cells. For example, the coding sequences could be further manipulated (e.g., ligated to other known linkers or modified by deleting non-coding sequences therefrom or altering nucleotide therein by other known techniques). The modified aggrecanase-related coding sequence could then be inserted into a known bacterial vector using procedures such as described in Taniguchi et al., Proc. Natl Acad. Sci. USA, 77:5230-5233 (1980). This exemplary bacterial vector could then be transformed into bacterial host cells and an aggrecanase-related protein expressed thereby. For a strategy for producing extracellular expression of aggrecanase-related proteins in bacterial cells, see, e.g. European patent application EPA 177,343.

[0035] Similar manipulations can be performed for the construction of an insect vector (See, e.g., procedures described in published European patent application 155,476) for expression in insect cells. A yeast vector could also be constructed employing yeast regulatory sequences for intracellular or extracellular expression of the factors of the present invention by yeast cells. (See, e.g., procedures described in published PCT application WO86/00639 and European patent application EPA 123,289).

[0036] A method for producing high levels of an aggrecanase-related protein of the invention in mammalian, bacterial, yeast or insect host cell systems may involve the construction of cells containing multiple copies of the heterologous aggrecanase-related gene. The heterologous gene is linked to an amplifiable marker, e.g., the dihydrofolate reductase (DHFR) gene for which cells containing increased gene copies can be selected for propagation in increasing concentrations of methotrexate (MTX) according to the procedures of Kaufman and Sharp, J. Mol. Biol., 159:601-629 (1982). This approach can be employed with a number of different cell types.

[0037] For example, a plasmid containing a DNA sequence for an aggrecanase-related protein of the invention in operative association with other plasmid sequences enabling expression thereof and the DHFR expression plasmid pAdA26SV(A)3 (Kaufman and Sharp, Mol. Cell. Biol., 2:1304 (1982)) can be co-introduced into DHFR-deficient CHO cells, DUKX-BII, by various methods including calcium phosphate coprecipitation and transfection, electroporation or protoplast fusion. DHFR expressing transformants are selected for growth in alpha media with dialyzed fetal calf serum, and subsequently selected for amplification by growth in increasing concentrations of MTX (e.g. sequential steps in 0.02, 0.2, 1.0 and 5 uM MTX) as described in Kaufman et al., Mol Cell Biol., 5:1750 (1983). Transformants are cloned, and biologically active aggrecanase expression is monitored by the assays described above. Aggrecanase protein expression should increase with increasing levels of MTX resistance. Aggrecanase proteins are characterized using standard techniques known in the art such as pulse labeling with 35S methionine or cysteine and polyacrylamide gel electrophoresis. Similar procedures can be followed to produce other related aggrecanase-related proteins.

[0038] IV. Production of Aggrecanase Proteins

[0039] Another aspect of the present invention provides a novel method for producing novel aggrecanase proteins. The method of the present invention involves culturing a suitable cell line, which has been transformed with a DNA sequence encoding an aggrecanase protein of the invention, under the control of known regulatory sequences. The transformed host cells are cultured and the aggrecanase proteins recovered and purified from the culture medium. The purified proteins are substantially free from other proteins with which they are co-produced as well as from other contaminants. The recovered purified protein is contemplated to exhibit proteolytic aggrecanase activity cleaving aggrecan. Thus, the proteins of the invention may be further characterized by the ability to demonstrate aggrecan proteolytic activity in an assay which determines the presence of an aggrecan-degrading molecule. These assays or the development thereof is within the knowledge of one skilled in the art. Such assays may involve contacting an aggrecan molecule and monitoring the production of aggrecan fragments (see for example, Hughes et al., Biochem J, 305:799-804(1995); Mercuri et al., J. Bio Chem., 274:32387-32395 (1999)).

[0040] Suitable cells or cell lines may be mammalian cells, such as Chinese hamster ovary cells (CHO). The selection of suitable mammalian host cells and methods for transformation, culture, amplification, screening, product production and purification are known in the art. (See, e.g., Gething and Sambrook, Nature, 293:620-625 (1981), Kaufman et al., Mol. Cell. Biol., 5(7):1750-1759 (1985); Howley et al., U.S. Pat. No. 4,419,446.) Another suitable mammalian cell line, which is described in the accompanying examples, is the monkey COS-1 cell line. The mammalian cell CV-1 may also be suitable.

[0041] Bacterial cells may also be suitable hosts. For example, the various strains of E. coil (e.g., HB101, MC1061) are well-known as host cells in the field of biotechnology. Various strains of B. subtilis, Pseudomonas, other bacilli and the like may also be employed in this method. For expression of the protein in bacterial cells, DNA encoding the propeptide of aggrecanase is generally not necessary.

[0042] Many strains of yeast cells known to those skilled in the art may also be available as host cells for expression of the polypeptides of the present invention. Additionally, where desired, insect cells may be utilized as host cells in the method of the present invention. See, e.g. Miller et al., Genetic Engineering, 8:277-298 (Plenum Press 1986).

[0043] Another aspect of the present invention provides vectors for use in the method of expression of these novel aggrecanase polypeptides. Preferably the vectors contain the full novel DNA sequences described above which encode the novel factors of the invention. Additionally, the vectors contain appropriate expression control sequences permitting expression of the aggrecanase protein sequences. Alternatively, vectors incorporating modified sequences as described above are also embodiments of the present invention. Additionally, the sequence of SEQ ID NO: 1, SEQ ID NO: 3 or other sequences encoding aggrecanase proteins could be manipulated to express composite aggrecanase proteins. Thus, the present invention includes chimeric DNA molecules encoding an aggrecanase protein comprising a fragment from SEQ ID NO: 1 or SEQ ID NO: 3 linked in correct reading frame to a DNA sequence encoding another aggrecanase protein.

[0044] The vectors may be employed in the method of transforming cell lines and contain selected regulatory sequences in operative association with the DNA coding sequences of the invention which are capable of directing the replication and expression thereof in selected host cells. Regulatory sequences for such vectors are known to those skilled in the art and may be selected depending upon the host cells. Such selection is routine and does not form part of the present invention.

[0045] V. Generation of Antibodies

[0046] The purified proteins of the present inventions may be used to generate antibodies, either monoclonal or polyclonal, to aggrecanase and/or other aggrecanase-related proteins, using methods that are known in the art of antibody production. Thus, the present invention also includes antibodies to aggrecanase or other related proteins. The antibodies include both those that block aggrecanase activity and those that do not. The antibodies may be useful for detection and/or purification of aggrecanase or related proteins, or for inhibiting or preventing the effects of aggrecanase. The aggrecanase of the invention or portions thereof may be utilized to prepare antibodies that specifically bind to aggrecanase.

[0047] The term “antibody” as used herein, refers to an immunoglobulin or a part thereof, and encompasses any protein comprising an antigen binding site regardless of the source, method of production, and characteristics. The term includes but is not limited to polyclonal, monoclonal, monospecific, polyspecific, non-specific, humanized, single-chain, chimeric, synthetic, recombinant, hybrid, mutated, and DCR-grafted antibodies. It also includes, unless otherwise stated, antibody fragments such as Fab, F(ab′)2, Fv, scFv, Fd, dAb, and other antibody fragments which retain the antigen binding function.

[0048] Antibodies can be made, for example, via traditional hybridoma techniques (Kohler and Milstein, Nature 256:495-499 (1975)), recombinant DNA methods (U.S. Pat. No. 4,816,567), or phage display techniques using antibody libraries (Clackson et al., Nature 352:624-628 (1991); Marks et al., J. Mol. Biol., 222:581-597 (1991)). For various other antibody production techniques, see Antibodies: A Laboratory Manual, eds. Harlow et al., Cold Spring Harbor Laboratory (1988).

[0049] An antibody “specifically” binds to at least one novel aggrecanase molecule of the present invention when the antibody will not show any significant binding to molecules other than at least one novel aggrecanase molecule. The term is also applicable where, e.g., an antigen binding domain is specific for a particular epitope, which is carried by a number of antigens, in which case the specific binding member (the antibody) carrying the antigen binding domain will be able to bind to the various antigens carrying the epitope. In this fashion it is possible that an antibody of the invention will bind to multiple novel aggrecanase proteins. Typically, the binding is considered specific when the affinity constant Ka is higher than 108 M−1. An antibody is said to “specifically bind” or “specifically react” to an antigen if, under appropriately selected conditions, such binding is not substantially inhibited, while at the same time non-specific binding is inhibited. Such conditions are well known in the art, and a skilled artisan using routine techniques can select appropriate conditions. The conditions are usually defined in terms of concentration of antibodies, ionic strength of the solution, temperature, time allowed for binding, concentration of non-related molecules (e.g., serum albumin, milk casein), etc.

[0050] Proteins are known to have certain biochemical properties including sections which are hydrophobic and sections which are hydrophilic. The hydrophobic sections would most likely be located in the interior of the structure of the protein while the hydrophilic sections would most likely be located in the exterior of the structure of the protein. It is believed that the hydrophilic regions of a protein would then correspond to antigenic regions on the protein. Knowing the location of the antigenic regions would enable one of ordinary skill in the art to then generate antibodies specific to that region. The hydrophobicity of SEQ ID NO. 3 was determined using GCG PepPlot. The results indicated that the n-terminus was hydrophobic presumably because of a signal sequence.

[0051] VI. Development of Inhibitors

[0052] Various conditions such as osteoarthritis are known to be characterized by degradation of aggrecan. Therefore, an aggrecanase protein of the present invention which cleaves aggrecan may be useful for the development of inhibitors of aggrecanase. The invention therefore provides compositions comprising an aggrecanase inhibitor. The inhibitors may be developed using the aggrecanase in screening assays involving a mixture of aggrecan substrate with the inhibitor followed by exposure to aggrecan. Inhibitors can be screened using high throughput processes, such as by screening a library of inhibitors. Inhibitors can also be made using three-dimensional structural analysis and/or computer aided drug design. The compositions may be used in the treatment of osteoarthritis and other conditions exhibiting degradation of aggrecan.

[0053] VII. Administration

[0054] Another aspect of the invention therefore provides pharmaceutical compositions containing a therapeutically effective amount of aggrecanase antibodies and/or inhibitors, in a pharmaceutically acceptable vehicle. Aggrecanase-mediated degradation of aggrecan in cartilage has been implicated in osteoarthritis and other inflammatory diseases. Therefore, these compositions of the invention may be used in the treatment of diseases characterized by the degradation of aggrecan and/or an up regulation of aggrecanase. The compositions may be used in the treatment of these conditions or in the prevention thereof.

[0055] The invention includes methods for treating patients suffering from conditions characterized by a degradation of aggrecan or preventing such conditions. These methods, according to the invention, entail administering to a patient needing such treatment, an effective amount of a composition comprising an aggrecanase antibody or inhibitor which inhibits the proteolytic activity of aggrecanase enzymes.

[0056] The antibodies and inhibitors of the present invention are useful to prevent, diagnose, or treat various medical disorders in humans or animals. In one embodiment, the antibodies can be used to inhibit or reduce one or more activities associated with the aggrecanase protein, relative to an aggrecanase protein not bound by the same antibody. Most preferably, the antibodies and inhibitors inhibit or reduce one or more of the activities of aggrecanase relative to the aggrecanase that is not bound by an antibody. In certain embodiments, the activity of aggrecanase, when bound by one or more of the presently disclosed antibodies, is inhibited at least 50%, preferably at least 60, 62, 64, 66, 68, 70, 72, 72, 76, 78, 80, 82, 84, 86, or 88%, more preferably at least 90, 91, 92, 93, or 94%, and even more preferably at least 95% to 100% relative to an aggrecanase protein that is not bound by one or more of the presently disclosed antibodies.

[0057] Generally, the compositions are administered so that antibodies/their binding fragments are given at a dose between 1 μg/kg and 20 mg/kg, 1 μg/kg and 10 mg/kg, 1 μg/kg and 1 mg/kg, 10 μg/kg and 1 mg/kg, 10 μg/kg and 100 μg/kg, 100 μg and 1 mg/kg, and 500 μg/kg and 1 mg/kg. Preferably, the antibodies are given as a bolus dose, to maximize the circulating levels of antibodies for the greatest length of time after the dose. Continuous infusion may also be used after the bolus does.

[0058] In another embodiment and for administration of inhibitors, such as proteins and small molecules, an effective amount of the inhibitor is a dosage which is useful to reduce the activity of aggrecanase to achieve a desired biological outcome. Generally, appropriate therapeutic dosages for administering an inhibitor may range from 5 mg to 100 mg, from 15 mg to 85 mg, from 30 mg to 70 mg, or from 40 mg to 60 mg. Inhibitors can be administered in one dose, or at intervals such as once daily, once weekly, and once monthly. Dosage schedules can be adjusted depending on the affinity for the inhibitor to the aggrecanase target, the half-life of the inhibitor, and the severity of the patient's condition. Generally, inhibitors are administered as a bolus dose, to maximize the circulating levels of inhibitor. Continuous infusions may also be used after the bolus dose.

[0059] Toxicity and therapeutic efficacy of such compounds can be determined by standard pharmaceutical procedures in cell cultures or experimental animals, e.g., for determining the LD50 (the dose lethal to 50% of the population) and the ED50 (the dose therapeutically effective in 50% of the population). The dose ratio between toxic and therapeutic effects is the therapeutic index and it can be expressed as the ratio LD50/ED50. Antibodies and inhibitors, which exhibit large therapeutic indices, are preferred.

[0060] The data obtained from the cell culture assays and animal studies can be used in formulating a range of dosage for use in humans. The dosage of such compounds lies preferably within a range of circulating concentrations that include the ED50 with little or no toxicity. The dosage may vary within this range depending upon the dosage form employed and the route of administration utilized. For any antibody and inhibitor used in the present invention, the therapeutically effective dose can be estimated initially from cell culture assays. A dose may be formulated in animal models to achieve a circulating plasma concentration range that includes the IC50 (i.e., the concentration of the test antibody which achieves a half-maximal inhibition of symptoms) as determined in cell culture. Levels in plasma may be measured, for example, by high performance liquid chromatography. The effects of any particular dosage can be monitored by a suitable bioassay. Examples of suitable bioassays include DNA replication assays, transcription-based assays, GDF protein/receptor binding assays, creatine kinase assays, assays based on the differentiation of pre-adipocytes, assays based on glucose uptake in adipocytes, and immunological assays.

[0061] The therapeutic methods of the invention include administering the aggrecanase inhibitor compositions topically, systemically, or locally as an implant or device. The dosage regimen will be determined by the attending physician considering various factors which modify the action of the aggrecanase protein, the site of pathology, the severity of disease, the patient's age, sex, and diet, the severity of any inflammation, time of administration and other clinical factors. Generally, systemic or injectable administration will be initiated at a dose which is minimally effective, and the dose will be increased over a preselected time course until a positive effect is observed. Subsequently, incremental increases in dosage will be made limiting such incremental increases to such levels that produce a corresponding increase in effect, while taking into account any adverse affects that may appear. The addition of other known factors, to the final composition, may also effect the dosage.

[0062] Progress can be monitored by periodic assessment of disease progression. The progress can be monitored, for example, by x-rays, MRI or other imaging modalities, synovial fluid analysis, and/or clinical examination. p VIII. Assays and Methods of Detection

[0063] The inhibitors and antibodies of the invention can be used in assays and methods of detection to determine the presence or absence of, or quantify aggrecanase in a sample. The inhibitors and antibodies of the present invention may be used to detect aggrecanase proteins, in vivo or in vitro. By correlating the presence or level of these proteins with a medical condition, one of skill in the art can diagnose the associated medical condition or determine its severity. The medical conditions that may be diagnosed by the presently disclosed inhibitors and antibodies are set forth above.

[0064] Such detection methods for use with antibodies are well known in the art and include ELISA, radioimmunoassay, immunoblot, western blot, immunofluorescence, immuno-precipitation, and other comparable techniques. The antibodies may further be provided in a diagnostic kit that incorporates one or more of these techniques to detect a protein (e.g., an aggrecanase protein). Such a kit may contain other components, packaging, instructions, or other material to aid the detection of the protein and use of the kit. When protein inhibitors are used in such assays, protein-protein interaction assays can be used.

[0065] Where the antibodies and inhibitors are intended for diagnostic purposes, it may be desirable to modify them, for example, with a ligand group (such as biotin) or a detectable marker group (such as a fluorescent group, a radioisotope or an enzyme). If desired, the antibodies (whether polyclonal or monoclonal) may be labeled using conventional techniques. Suitable labels include fluorophores, chromophores, radioactive atoms, electron-dense reagents, enzymes, and ligands having specific binding partners. Enzymes are typically detected by their activity. For example, horseradish peroxidase can be detected by its ability to convert tetramethylbenzidine (TMB) to a blue pigment, quantifiable with a spectrophotometer. Other suitable binding partners include biotin and avidin or streptavidin, IgG and protein A, and the numerous receptor-ligand couples known in the art.

[0066] The following non-limiting examples illustrate practice of the present invention in isolating and characterizing human aggrecanase and other aggrecanase-related proteins, obtaining the human proteins and expressing the proteins via recombinant techniques.

EXAMPLES

Example 1

Isolation of DNA

[0067] Potential novel aggrecanase family members were identified using a database screening approach. Aggrecanase-1 (Science, 284:1664-1666 (1999)) has at least six domains: signal, propeptide, catalytic domain, disintegrin, tsp and c-terminal. The catalytic domain contains a zinc binding signature region, TAAHELGHVKF (SEQ ID NO: 7) and a “MET turn” which are responsible for protease activity. Substitutions within the zinc binding region in the number of the positions still allow protease activity, but the histidine (H) and glutamic acid (E) residues must be present. The thrombospondin domain of Aggrecanase-1 is also a critical domain for substrate recognition and cleavage. It is these two domains that determine our classification of a novel aggrecanase family member. The protein sequence of the Aggrecanase-1 DNA sequence was used to query against the GenBank ESTs focusing on human ESTs using TBLASTN. The resulting sequence Genbank EST AJ403134 was the starting point in the effort to identify a full length sequence for potential family members. The nucleotide sequence of the aggrecanase of the present invention is comprised of one EST, EST-16, that contains homology over the catalytic domain and zinc binding motif of Aggrecanase-1. The structure of the entire gene was reconstructed using public DNA sequences and a Genscan prediction of the gene found in the Celera Genome Browser (Rockville, Md., USA).

[0068] The human aggrecanase gene was isolated using a PCR strategy with tissue sources determined by probing a Clontech Human Multiple Tissue Expression Array (MTE) (Palo Alto, Calif., USA). The probe for the MTE was generated using PCR primers that amplified the 3′ end of the EST-16 Genscan predicted sequence, SEQ ID NO: 1 from bp 2977-4080. Primer sequences were as follows: 5′ primer sequence—CTGGAGGAGATGGACACCTATGAGTG (SEQ ID NO: 8) and 3′ primer sequence—AAATGGGCGCGGCCGCTTATCTCAGGTCTTCTCCAGGTTGCCCTTTG (SEQ ID NO: 9) (this primer incorporated a stop codon (TAA), a Not 1 sequence (GCGGCCGC) and an 8 bp tail (GCCCATTT) onto the end of the EST-16 Genscan predicted sequence). A Wyeth human uterus oligo dT-primed cDNA library (FL60) was used as substrate with the PCR primer set to amplify the appropriate 1103 bp EST-16 fragment (SEQ ID NO: 1, bp 2977-4080). The Advantage-GC 2 PCR Kit from Clontech was used for the PCR reactions. Reaction conditions were those recommended in the user manual; with the following exceptions: the amount of GC Melt used was 5 μl per 50 μl reaction; the amount of non-linearized library used was 1 ng/μl reaction; and the amount of each oligo used was 0.1 pmol/μl reaction. Cycling conditions were as follows: 94° C. for 1 min, one cycle; followed by 40 cycles consisting of 95° C. for 15 sec/68° C. for 3 min. The PCR fragment representing the 3′ end of EST-16 was radiolabelled using the Ready-To-Go DNA Labelling Beads (-dCTP) from Amersham Pharmacia Biotech (Piscataway, N.J., USA) per the manufacturer's instructions. The radiolabelled fragment was purified away from primers and unincorporated radionucleotides using a Nick column from Amersham Pharmacia Biotech per the manufacturer's instructions and then used to probe the MTE. Manufacturer's conditions for hybridization of the MTE using a radiolabelled cDNA probe were followed. EST-16 was found to be expressed in a leukemia K-562 cell line, in an adenocarcinoma SW480 cell line and in fetal lung tissue. Marathon-Ready cDNAs corresponding to these cell lines or tissue were purchased from Clontech: human colorectal adenocarcinoma SW480 cell line, ATCC#CCL228; human fetal lung pooled from 9 male/female Caucasian fetuses, ages 20-25 weeks; and human leukemia, chronic myelogenous K-562 cell line, ATCC#CCL243. PCR primers to the 5′ and internal portions of the EST-16 Genscan predicted sequence were designed. Three overlapping pieces of EST-16 were amplified using the following primer sets. The first PCR primer set amplified from bp 1-1227 of the EST-16 Genscan predicted sequence, SEQ ID NO: 1; 5′ primer sequence—AAATGGGCGMTTCCCACCATGGCTCCACTCCGCGCGCTGCTGTCCTA (SEQ ID NO: 10) (this primer incorporated an 8 bp tail (AAATGGGC), an EcoR1 sequence (GAATTC) and a Kozak sequence (CCACC) upstream of the initiator Met (ATG)) and the 3′ primer sequence—GTAGCTGCCTGGCTTGGCATGCCG (SEQ ID NO: 11). The second primer set amplified from bp 1175-3068 of the EST-16 Genscan predicted sequence, SEQ ID NO: 1; 5′ primer sequence—ACCAGCTGGGCGACACAGAGCGGMGC (SEQ ID NO: 12) and the 3′ primer sequence—CCGTATTTGGTGMCTGGATCCCTCC (SEQ ID NO: 13). The third PCR primer set amplified from bp 2977-4080 of the EST-16 Genscan predicted sequence; 5′ primer sequence—CTGGAGGAGATGGACACCTATGAGTG (SEQ ID NO: 8) and 3′ primer sequence—AAATGGGCGCGGCCGCTTATCTCAGGTCTTCTCCAGGTTGCCCTTTG (SEQ ID NO: 9) (this primer incorporated a stop codon (TAA), a Not1 sequence (GCGGCCGC), and an 8 bp tail (GCCCATTT) onto the end of the EST-16 Genscan predicted sequence). The Advantage-GC 2 PCR Kit from Clontech was used to amplify the EST-16 gene products. Reaction conditions were those recommended in the user manual; with the following exceptions: the amount of GC Melt used was 5 μl per 50 μl reaction; the amount of Marathon cDNA used was 0.01 ng/μl of reaction; and the amount of each oligo used was 0.1 pmol/μl. Cycling conditions were as follows: 94° C. for 1 min, one cycle; followed by 40 cycles consisting of 95° C. for 15 sec/68° C. for 3 min. Each of the primer pairs were used in PCR amplification reactions containing each of the 3 Marathon cDNAs. PCR products resulting from the amplifications were ligated into the pT-Adv vector using the AdvanTAge PCR Cloning Kit, per manufacturer's instructions. Ligated products were transformed into ElectroMAX DH10B cells from Life Technologies (Carlsbad, Calif., USA). Cloned PCR fragments of EST-16 were sequenced to determine fidelity. PCR products with the correct sequence were moved from the pT-Adv vector into the Cos expression vector pED6-dpc2 and a full-length version of EST-16 was constructed as set forth in SEQ ID NO: 3. The full-length sequence for EST-16 was the consensus sequence derived from the EST-16 Genscan sequence and the PCR products generated for EST-16 from each of the 3 unique Clontech Marathon cDNAs. The 5′ and 3′ ends of EST-16 sequence were confirmed by RACE PCR using the Clontech Marathon cDNA Amplification Kit. The same Marathon cDNA sources used to PCR the EST-16 gene were the substrates for the RACE reactions. 3′ RACE primers used were; GSP1—CTACAAGTACGTCATCCATGAGGACC (SEQ ID NO: 14) and GSP2—CTGGAGGAGATGGACACCTATGAGTG (SEQ ID NO: 8). Two sets of 5′ RACE primers were used; the first set=GSP1—AATGAAGAAGTCGGTGCTGTCTGTGC (SEQ ID NO: 15) and GSP2—AGTGACACCTCCAGTGTACACACACT (SEQ ID NO: 16). The second set=GSP1—AATGAAGAAGTCGGTGCTGTCTGTGC (SEQ ID NO: 15) and GSP2—GAAAATCCTCCTGCCACTCCACTGAG (SEQ ID NO: 17). The Advantage-GC2 PCR Kit from Clontech was used to set up nested RACE reactions following instructions in the user manual for the Marathon cDNA Amplification Kit; the amount of GC Melt used was 5 μl per 50 μl reaction, and the amount the GSP oligos used was 0.1 pmol/μl. In all cases GSP1 primers were used for the first round of PCR and GSP2 primers were used for the nested reactions. Information from the 5′ RACE confirmed the EST-16 Genscan predicted initiator Met. Information from the 3′ RACE revealed an error in the sequence used for the 3′ end of EST-16. The sequence was repaired using synthetic oligos from bp 3585 to the end of the full-length EST-16 (bp 3683). The synthetic oligos inserted a Not1 sequence following the naturally occuring stop codon (TGA). This synthetic sequence reflected the stop predicted by a revised copy of the Genscan predicted sequence and confirmed by RACE PCR.

[0069] The nucleotide sequence is set forth in SEQ ID NO: 1 from nucleotide #1 to #4080. The full length confirmed sequence is set forth in SEQ ID NO: 3. The amino acid sequence is set forth in SEQ ID NO: 5 from amino acid #1-#1360. EST-16 sequence as set forth in SEQ ID NO: 3 has been deposited in the American Type Culture Collection 10801 University Blvd. Manassas, Va. 20110-2209 USA on May 30, 2001 as ATCC No. PTA 3410.

[0070] The aggrecanase nucleotide sequence of the invention can be used to design probes for further screening for full length clones containing the isolated sequence.

Example 2

Expression of Aggrecanase

[0071] In order to produce murine, human or other mammalian aggrecanase-related proteins, the DNA encoding it is transferred into an appropriate expression vector and introduced into mammalian cells or other preferred eukaryotic or prokaryotic hosts including insect host cell culture systems by conventional genetic engineering techniques. Expression system for biologically active recombinant human aggrecanase are contemplated to be stably transformed mammalian cells, insect, yeast or bacterial cells. Expression of aggrecan may be found in larynx carcinoma, kidney tumor, and ovary tissue.

[0072] One skilled in the art can construct mammalian expression vectors by employing a sequence comprising SEQ ID NOS: 1, 3, or other DNA sequences encoding aggrecanase-related proteins or other modified sequences and known vectors, such as pCD (Okayama et al., Mol. Cell Biol., 2:161-170 (1982)), pJL3, pJL4 (Gough et al., EMBO J., 4:645-653 (1985)) and pMT2 CXM.

[0073] The mammalian expression vector pMT2 CXM is a derivative of p91023(b) (Wong et al., Science, 228:810-815 (1985)) differing from the latter in that it contains the ampicillin resistance gene in place of the tetracycline resistance gene and further contains a Xhol site for insertion of cDNA clones. The functional elements of pMT2 CXM have been described (Kaufman, Proc. Natl. Acad. Sci. USA, 82:689-693 (1985)) and include the adenovirus VA genes, the SV40 origin of replication including the 72 bp enhancer, the adenovirus major late promoter including a 5′ splice site and the majority of the adenovirus tripartite leader sequence present on adenovirus late mRNAs, a 3′ splice acceptor site, a DHFR insert, the SV40 early polyadenylation site (SV40), and pBR322 sequences needed for propagation in E. coli.

[0074] Plasmid pMT2 CXM is obtained by EcoR1 digestion of pMT2-VWF, which has been deposited with the American Type Culture Collection (ATCC), Rockville, Md. (USA) under accession number ATCC 67122. EcoR1 digestion excises the cDNA insert present in pMT2-VWF, yielding pMT2 in linear form which can be ligated and used to transform E. coli HB 101 or DH-5 to ampicillin resistance. Plasmid pMT2 DNA can be prepared by conventional methods. pMT2 CXM is then constructed using loopout/in mutagenesis (Morinaga, et al., Biotechnology, 84: 636 (1984)). This removes bases 1075 to 1145 relative to the Hind III site near the SV40 origin of replication and enhancer sequences of pMT2. In addition it inserts the following sequence: 5′ PO-CATGGGCAGCTCGAG-3′ (SEQ ID NO: 18) at nucleotide 1145. This sequence contains the recognition site for the restriction endonuclease Xho I. A derivative of pMT2CXM, termed pMT23, contains recognition sites for the restriction endonucleases Pstl, Eco RI, Sall and Xhol. Plasmid pMT2 CXM and pMT23 DNA may be prepared by conventional methods.

[0075] pEMC2β031 derived from pMT21 may also be suitable in practice of the invention. pMT21 is derived from pMT2 which is derived from pMT2-VWF. As described above EcoRI digestion excises the cDNA insert present in pMT-VWF, yielding pMT2 in linear form which can be ligated and used to transform E. Coli HB 101 or DH-5 to ampicillin resistance. Plasmid pMT2 DNA can be prepared by conventional methods.

[0076] pMT21 is derived from pMT2 through the following two modifications. First, 76 bp of the 5′ untranslated region of the DHFR cDNA including a stretch of 19 G residues from G/C tailing for cDNA cloning is deleted. In this process, a Xhol site is inserted to obtain the following sequence immediately upstream from DHFR: 5′—

2(SEQ ID NO:19)CTGCAGGCGAGCCTGAATTCCTCGAGCCATCATG-3′PstI Eco RI XhoI

[0077] Second, a unique Clal site is introduced by digestion with EcoRV and Xbal, treatment with Klenow fragment of DNA polymerase 1, and ligation to a Clal linker (CATCGATG). This deletes a 250 bp segment from the adenovirus associated RNA (VAI) region but does not interfere with VAI RNA gene expression or function. pMT21 is digested with EcoRI and Xhol, and used to derive the vector pEMC2B1.

[0078] A portion of the EMCV leader is obtained from pMT2-ECAT1 (S. K. Jung, et al., J. Virol 63:1651-1660 (1989)) by digestion with Eco RI and Pstl, resulting in a 2752 bp fragment. This fragment is digested with Taql yielding an Eco RI-Taql fragment of 508 bp which is purified by electrophoresis on low melting agarose gel. A 68 bp adapter and its complementary strand are synthesized with a 5′ Taql protruding end and a 3′ Xhol protruding end which has the following sequence:

3(SEQ ID NO:20)5′-CGAGGTTAAAAAACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTT TaqITCCTTTGAAAAACACGATTGC-3′ XhoI

[0079] This sequence matches the EMC virus leader sequence from nucleotide 763 to 827. It also changes the ATG at position 10 within the EMC virus leader to an ATT and is followed by a Xhol site. A three way ligation of the pMT21 Eco RI-Xhol fragment, the EMC virus EcoRI-Taql fragment, and the 68 bp oligonucleotide adapter Taql-Xhol adapter resulting in the vector pEMC2β1.

[0080] This vector contains the SV40 origin of replication and enhancer, the adenovirus major late promoter, a cDNA copy of the majority of the adenovirus tripartite leader sequence, a small hybrid intervening sequence, an SV40 polyadenylation signal and the adenovirus VA I gene, DHFR and β-lactamase markers and an EMC sequence, in appropriate relationships to direct the high level expression of the desired cDNA in mammalian cells.

Example 3

Biological Activity of Expressed Aggrecanase

[0081] To measure the biological activity of the expressed aggrecanase-related proteins obtained in Example 2 above, the proteins are recovered from the cell culture and purified by isolating the aggrecanase-related proteins from other proteinaceous materials with which they are co-produced as well as from other contaminants. Purification is carried out using standard techniques known to those skilled in the art. The purified protein is assayed in accordance with the following assays:

[0082] Assays specifically to determine if the protein is an enzyme capable of cleaving aggrecan at the aggrecanase cleavage site:

[0083] 1. Flourescent peptide assay: Expressed protein is incubated with a synthetic peptide which encompasses amino acids at the aggrecanase cleavage site of aggrecan. One side of the synthetic peptide has a flourophore and the other a quencher. Cleavage of the peptide separates the flourophore and quencher and elicits flourescence. From this assay it can be determined that the expressed protein can cleave aggrecan at the aggrecanase site, and relative flourescence tells the relative activity of the expressed protein.

[0084] 2. Neoepitope western: Expressed protein is incubated with intact aggrecan. After several biochemical manipulations of the resulting sample (dialysis, chondroitinase treatment, lyophilization and reconstitution) the sample is run on an SDS PAGE gel. The gel is incubated with an antibody that only recognizes a site on aggrecan exposed after aggrecanase cleavage. The gel is transferred to nitrocellulose and developed with a secondary antibody to result in bands running at a molecular weight consistent with aggrecanase generated cleavage products of aggrecan. This assay, called a western assay, shows whether the expressed protein cleaved native aggrecan at the aggrecanase cleavage site, and also provides the molecular weight of the cleavage products. Relative density of the bands can give some idea of relative aggrecanase activity.

[0085] Assay to determine if an expressed protein can cleave aggrecan anywhere in the protein (not specific to the aggrecanase site):

[0086] 3. Aggrecan ELISA: Expressed protein is incubated with intact aggrecan which had been previously adhered to plastic wells. The wells are washed and then incubated with an antibody that detects aggrecan. The wells are developed with a secondary antibody. If there is the original amount of aggrecan remaining in the well, the antibody will densely stain the well. If aggrecan was digested off the plate by the expressed protein, the antibody will demonstrate reduced staining due to reduced aggrecan concentration. This assay tells whether an expressed protein is capable of cleaving aggrecan (anywhere in the protein, not only at the aggrecanase site) and can determine relative aggrecan cleaving.

[0087] Protein analysis of the purified proteins is conducted using standard techniques such as SDS-PAGE acrylamide (Laemmli, Nature 227:680 (1970)) stained with silver (Oakley, et al., Anal Biochem. 105:361 (1980)) and by immunoblot (Towbin, et al., Proc. Natl. Acad. Sci. USA 76:4350 (1979)). Using the above described assays, expressed aggrecanase-related proteins are evaluated for their activity and useful aggrecanase-related molecules are identified.

Example 4

Preparation of Antibodies

[0088] An antibody against a novel aggrecanase molecule is prepared. To develop an antibody capable of inhibiting aggrecanase activity, a group of mice are immunized every two weeks with a novel aggrecanase protein mixed in Freunds complete adjuvant for the first two immunizations, and incomplete Freunds adjuvant thereafter. Throughout the immunization period, blood is sampled and tested for the presence of circulating antibodies. At week 9, an animal with circulating antibodies is selected, immunized for three consecutive days, and sacrificed. The spleen is removed and homogenized into cells. The spleen cells are fused to a myeloma fusion partner (line P3-x63-Ag8.653) using 50% PEG 1500 by an established procedure (Oi & Herzenberg, Selected Methods in Cellular Immunology, W. J. Freeman Co., San Francisco, Calif., at 351 (1980)). The fused cells are plated into 96-well microtiter plates at a density of 2×105 cells/well. After 24 hours, the cells are subjected to HAT selection (Littlefield, Science, 145: 709 (1964)) effectively killing any unfused and unproductively fused myeloma cells.

[0089] Successfully fused hybridoma cells secreting anti-aggrecanase antibodies are identified by solid and solution phase ELISAs. Novel aggrecanase protein is prepared from CHO cells as described above and coated on polystyrene (for solid phase assays) or biotinylated (for a solution based assay). Neutralizing assays are also employed where aggrecan is coated on a polystyrene plate and biotin aggrecanase activity is inhibited by the addition of hybridoma supernatant. Results identify hybridomas expressing aggrecanase antibodies. These positive clones are cultured and expanded for further study. These cultures remain stable when expanded and cell lines are cloned by limiting dilution and cryopreserved.

[0090] From these cell cultures, a panel of antibodies is developed that specifically recognize aggrecanase proteins. Isotype of the antibodies is determined using a mouse immunoglobulin isotyping kit (Zymed™ Laboratories, Inc., San Francisco, Calif.).

Example 5

Method of Detecting Level of Aggrecanase

[0091] The anti-aggrecanase antibody prepared according to Example 4 can be used to detect the level of aggrecanase in a sample The antibody can be used in an ELISA, for example, to identify the presence or absence, or quantify the amount of, aggrecanase in a sample. The antibody is labeled with a fluorescent tag. In general, the level of aggrecanase in a sample can be determined using any of the assays disclosed in Example 3.

Example 6

Method of Treating a Patient

[0092] The antibody developed according to Example 4 can be administered to patients suffering from a disease or disorder related to the loss of aggrecan, or excess aggrecanase activity. Patients take the composition one time or at intervals, such as once daily, and the symptoms and signs of their disease or disorder improve. For example, loss of aggrecan would decrease or cease and degradation of articular cartilage would decrease or cease. Symptoms of osteoarthritis would be reduced or eliminated. This shows that the composition of the invention is useful for the treatment of diseases or disorders related to the loss of aggrecan, or excess aggrecanase activity. The antibodies can also be used with patients susceptible to osteoarthritis, such as those who have a family history or markers of the disease, but have not yet begun to suffer its effects.

4Patient'sRoute ofPredictedConditionAdministrationDosageFrequencyResultsOsteoarthritisSubcutaneous500 μg/kgDailyDecrease insymptoms″″ 1 mg/kgWeekly″″Intramuscular500 μg/kgDaily″″″ 1 mg/kgWeekly″″Intravenous500 μg/kgDaily″″″ 1 mg/kgWeekly″Family History ofSubcutaneous500 μg/kgDailyPreventionOsteoarthritisof condition″Intramuscular500 μg/kgDaily″″Intravenous500 μg/kgDaily″

[0093] The foregoing descriptions detail presently preferred embodiments of the present invention. Numerous modifications and variations in practice thereof are expected to occur to those skilled in the art upon consideration of these descriptions. Those modifications and variations are believed to be encompassed within the claims appended hereto. All of the documents cited in this application are incorporated by reference in their entirety. Additionally, all sequences cited in databases and all references disclosed are incorporated by reference in their entirety.

	Number	Date	Country
	60308386	Jul 2001	US
	60315887	Aug 2001	US

Aggrecanase molecules

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