Patent Application
20240018061
The present invention relates to an agricultural composition for improving silicon uptake and distribution in plants, to a method for preparing the same, to a formulation comprising the agricultural composition, to a method of supplying silicon to plants and to the use of the agricultural composition as a fertiliser.
Plants need a range of nutrients for healthy growth. These include macronutrients such as nitrogen, phosphorus, potassium, carbon and water, secondary nutrients such as calcium, magnesium, sodium, chloride and sulphur, as well as micronutrients, which include copper, cobalt, iron, manganese, boron, molybdenum, zinc, silicon and nickel.
Soils typically contain more than 50% silicon with the majority being in the form of oxides and clay colloids, meaning most of the silicon in soil is not plant available. Of the silicon that is available, typically as H4SiO4 (Silicic acid) in soil solution, plants must compete with bacteria and diatoms for access to the pool of soluble silicon.
Plants also vary is their ability to take up silicon. “Strong accumulators” are plants which take up silicon at a higher level than present in the transpiration stream e.g., rice and sugar cane. “Intermediate accumulators” are those which take up silicon at the same level present in soil solution, e.g., wheat barley and most monocots, whereas “weak accumulators” are plants which take up silicon at lower levels than are present in soil solution. Most dicots are weak accumulators.
Silicon is known to form crystal layers called ‘opals’ made of amorphous silica in the cuticle. Opals confer physical strength protecting against pest and disease attack and preventing water loss. Silicon is also known to be responsible for triggering a range of metabolic functions which allows plants to cope and survive in response to abiotic stressors such as drought, lodging, extreme temperatures (low and high), UV light and increased salinity. However, silicon can only trigger metabolic activity when in soluble form, and once converted to opals, it becomes permanently inactive.
Silicon-based fertilisers are known, but are limited by the plant's ability to take up and transport silicon. This is because silicon can only move through the plant with water via xylem (it's not phloem mobile) and because plants vary dramatically in their ability to express influx and efflux proteins which are required to load and unload silicon from xylem. As a consequence, silicon is poorly distributed through the plant and tends to be converted into opals in the immediate areas where it is applied. This leads to uneven plant growth and strength and reduced quantities of metabolically active silicon. It also means that such fertilisers need to be applied more frequently and at higher application rates in order to obtain a growth response, thereby increasing costs.
In light of the above it is an object of embodiments of the present to provide a composition which increases the uptake of silicon by plants, particularly by weak accumulators.
It is another object of embodiments of the present invention to provide a composition which improves the transport and distribution of silicon within plants.
It is also an object of embodiments of the present invention to provide a composition which allows silicon to remain metabolically active for longer.
It is a further object of embodiment the present invention to provide a composition which improves a plant's strength and resistance to biotic and abiotic stressors.
It is still a further object of embodiments of the present invention to provide a composition which improves silicon uptake and distribution in plants at reduced cost.
According to a first aspect of the invention there is provided an agricultural composition which comprises (i) a water-soluble source of silicon and (ii) a silicon transport stimulant comprising an aryl substituted urea.
By administering the agricultural composition to plants significant improvements in silicon uptake and distribution can be obtained. The agricultural composition also enables silicon in treated plants to remain metabolically active for longer, stimulating growth and the production of protective metabolites. This in turn has led to observable improvements in plant growth, plant growth rate, plant strength, plant resistance to biotic and abiotic stressors and post-harvest storage. Moreover, due the improved uptake and distribution, the agricultural composition can be applied less frequently and at reduced application rates compared to conventional silicon-based fertilisers.
Advantageously, the application of the agricultural composition to plants enables improvements in plant growth to be obtained relative to treatments where a soluble source of silicon and a silicon transport stimulant comprising an aryl substituted urea are applied separately and in subsequent application steps. This has been attributed to the synergistic effect that is obtained when the soluble source of silicon and the silicon transport stimulant are applied concurrently in the same composition. In this respect, neither the silicon nor the silicon transport stimulant behaved the same alone as in combination.
The water-soluble source of silicon may comprise a water-soluble salt of silicon and/or silicic acid. In particular, the water-soluble salt of silicon may comprise potassium silicate and/or sodium silicate.
The water-soluble source of silicon may be present in the composition in an amount from 2 to 95% w/w of the composition. In some embodiments the water-soluble source of silicon may be present in the composition in an amount from 25 to 95 w/w %. In other embodiments it may be present in an amount from 50 to 95 w/w % or from 70 to 90 w/w %. Suitably, it may be present in an amount from 75 to 85% w/w %, e.g., around 80 w/w %.
The silicon transport stimulant may comprise a phenyl substituted urea. In some embodiments the silicon transport stimulant may comprise an unsymmetrically and/or a symmetrically substituted diphenyl urea or a derivative thereof.
The silicon transport stimulant may comprise diphenyl urea (DPU), N-(2-Chloro-4-pyridyl)-N′-phenylurea 2-nitro DPU (NDPU), mono- or di-methyl DPU, mono- or di-ethyl DPU or a combination thereof. Treating plants with a composition which contains one or more of the above silicon transport stimulants enables significant improvements in plant growth, speed of growth and resistance to biotic and abiotic stressors to be obtained.
The silicon transport stimulant may be present within the composition at a concentration of at least 10 ppm. In some embodiments the silicon transport stimulant may be present at a concentration of 10 to 2000 ppm, 10 to 1500 ppm, 10 to 1000 ppm, 10 to 750 ppm, 10 to 500 ppm or 10 to 250 ppm. Suitably, the silicon transport stimulant may be present at concentration of 20 to 200 ppm, 50 to 200 ppm or 100 to 200 ppm. Improved silicon uptake and transport can be obtained even at low silicon transport stimulant concentrations meaning improvements in plant growth and resistance can be obtained at relatively low cost.
If the composition comprises two or more silicon transport stimulants, e.g., DPU and CPPU, then the amount of each silicon transport stimulant may be substantially the same. For example, the silicon transport stimulants may be provided in a 1:1 ratio, meaning a composition containing 100 ppm of silicon transport stimulants would comprise 50 ppm of a first silicon transport stimulant such as DPU and 50 ppm of a second silicon transport stimulant such as CPPU. The silicon transport stimulants may be provided in a ratio between 1:10 and 10:1. In some embodiments the silicon transport stimulants may be provided in a ratio between 1:8 and 8:1. In other embodiments, the silicon transport stimulants may be provided in a ratio between 1:6 and 6:1. Suitably, the silicon transport stimulants may be provided in a ratio between 1:4 and 4:1. In particular, the silicon transport stimulants may be provided in a ratio between 1:2 and 2:1.
The composition may comprise one or more of the following agriculturally acceptable components: water, additional nutrient material, weak acids, metabolic stimulating agents, colouring agents, carriers or excipients, emulsifiers, thickeners, suspension agents, dispersion agents and wetting agents.
When additional nutrient materials are present in the composition, they are may be in the form of a water-soluble salt. The water-soluble salt of a nutrient mineral may be a water-soluble salt of another secondary nutrient, such as calcium, magnesium, sodium, chloride and sulphur, or a micronutrient, in particular, copper, cobalt, iron, manganese, boron, molybdenum, zinc, and nickel. Specific examples of water-soluble nutrient salts include nitrates, sulphates and chlorides. In particular, zinc nitrate, iron sulphate, zinc sulphate, magnesium sulphate, manganese sulphate, iron nitrate or manganese nitrate.
Suitable emulsifiers for use in the composition may include any known agriculturally acceptable emulsifiers. In particular, the emulsifier may comprise a surfactant such as: alkylaryl sulphonates, ethoxylated alcohols, polyalkoxylated butyl ethers, calcium alkyl benzene sulphonates, polyalkylene glycol ethers and butyl polyalkylene oxide block copolymers as are known in the art. Nonyl phenol emulsifiers such as Triton N57™ are particular examples of emulsifiers, which may be used in the composition, as are polyoxyethylene sorbitan esters such as polyoxyethylene sorbitan monolaurate (sold by ICI under the trade name “Tweet™”). In some embodiments, natural organic emulsifiers may be used in the composition, particularly for organic farming applications. Coconut oils such as coconut diethanolamide is an example of such a compound. Palm oil products such as lauryl stearate may also be used.
Thickeners which may be present in the composition include gums, for example xanthan gum, or lignosulphonate complexes, as are known in the art.
Suitable suspension agents which may be included in the composition include hydrophilic colloids (such as polysaccharides, polyvinylpyrrolidone or sodium carboxymethylcellulose) and swelling clays (such as bentonite or attapulgite).
Suitable wetting agents for use in the agricultural composition include cationic, anionic, amphoteric or non-ionic surfactants.
The composition may comprise a weak acid. A “weak acid” refers to a weak organic acid such as acetic acid, citric acid, humic acid, fulvic acid or propanoic acid. The presence of these acids improves the uptake of nutrients, and particularly nitrogen and secondary or micronutrients, by plants.
The composition may additionally comprise urea, i.e., in addition to the aryl substituted urea. The composition may comprise 5 to 15 w/w % urea. Suitably, the composition may comprise 8 to 12 w/w % urea. Advantageously, the addition of urea improves the mixability and stability of the composition.
The agricultural composition is suitable for use on most crops, but in particular can be used for the treatment of greenhouse crops, vegetables, herbs, crops and floriculture crops. The composition is particularly suitable for treating crops that are known to be intermediate and weak accumulators which typically contain 1-3% Si and 0.1-0.5% Si respectively.
The agricultural composition may exhibit insecticidal and/or acaricidal activity. For example, the agricultural composition may be effective against thrips, whitefly, aphids, spider mites, mealybugs, scale insects, psylla and mites. However, the agricultural composition does not exhibit any herbicidal activity which would either kill or inhibit the growth of plants. In contrast, the agricultural composition has the opposite effect and promotes plant growth.
According to a second aspect of the invention there is provided a formulation for administration to plants or to an environment of plants, the formulation comprising the composition according to the first aspect of the invention and a medium in which the composition is dispersed or dissolved.
The medium may comprise water or a water-miscible liquid. The water miscible liquid may comprise n-propanol, methanol, ethanol or isopropyl alcohol.
The composition according to the first aspect of the invention and the formulation may be used either alone or in conjunction with other agrochemicals such as fertilisers, fungicides, insecticides or acaricides. Preferably the composition and formulation are not used in conjunction with herbicides since this will prevent the growth promoting properties of the composition and formulation from being realised.
According to a third aspect of the invention there is provided a method for supplying silicon to plants, the method comprising the step of applying the composition according to the first aspect of the invention or the formulation according to the second aspect of the invention to the plants or to an environment thereof. The method according to the third aspect of the invention may, as appropriate, include any or all features described in relation to the first and second aspects of the invention.
By applying the composition according to the first aspect of the invention to plants, the water-soluble source of silicon and the silicon transport stimulant are supplied together in a single step. This has enabled improvements in plant growth to be obtained as well as reducing the frequency and cost of applying agricultural compositions to plants.
Since application of the composition enables improvements in silicon uptake and distribution within plants to be obtained, it can be applied less frequently and at reduced application rates. For example, the composition may be applied at intervals of at least four weeks, e.g., every 4-6 weeks, whereas known products with similar silicon contents need to applied every 1-2 weeks. The composition may be applied at an application rate of 0.5-4 L/Ha. In some embodiments the application rate may be 0.5-2.5 L/Ha. Suitably, the application rate may be 0.5-1.5 L/Ha, e.g., around 1 L/Ha which is much lower than the application rates (5L/Ha) for known products with similar silicon contents. The agricultural composition may be applied to plants, in particular crop plants, in any conventional manner, e.g. by soil or foliar application. They may be applied to root systems, stems, seeds, grains, tubers, flowers, fruit, etc. as required. Examples of means of application include spraying, e.g., by means of an electrostatic or other conventional sprayer, or drip irrigation methods or fertigation systems, which involve application directly to the soil, so as to allow calcium uptake through the roots.
According to a fourth aspect of the invention there is provided a method for enhancing the uptake of silicon by plants, the method comprising the step of applying the composition according to the first aspect of the invention or the formulation according to the second aspect of the invention to the plants or to an environment thereof. The method according to the fourth aspect of the invention may, as appropriate, include any or all features described in relation to the first and second aspects of the invention.
According to a fifth aspect of the invention there is provided a method for reducing physical damage by pests, the method comprising the step of applying the composition according to the first aspect of the invention or the formulation according to the second aspect of the invention to the plants or to an environment thereof. The method according to the fifth aspect of the invention may, as appropriate, include any or all features described in relation to the first and second aspects of the invention.
According to a sixth aspect of the invention there is provided a method for improving shelf life of a harvested crop by enhancing silicon distribution to the harvested parts of a plant, the method comprising the step of applying the composition according to the first aspect of the invention or the formulation according to the second aspect of the invention to the plants or to an environment thereof. The method according to the sixth aspect of the invention may, as appropriate, include any or all features described in relation to the first and second aspects of the invention.
According to a seventh aspect of the invention there is provided a method for preventing or alleviating disease or infection in plants by improving distribution and efficacy of applied silicon, the method comprising the step of applying the composition according to the first aspect of the invention or the formulation according to the second aspect of the invention to the plants or to an environment thereof. The method according to the seventh aspect of the invention may, as appropriate, include any or all features described in relation to the first and second aspects of the invention.
According to an eighth aspect of the invention there is provided a method for improving plant growth during conditions of abiotic stress, the method comprising the step of applying the composition according to the first aspect of the invention or the formulation according to the second aspect of the invention to the plants or to an environment thereof. The method according to the eighth aspect of the invention may, as appropriate, include any or all features described in relation to the first and second aspects of the invention.
According to a ninth aspect of the invention there is provided a method for improving plant growth rate, the method comprising applying the composition according to first aspect of the invention or the formulation according to the second aspect of the invention to the plant or to an environment thereof. The method according to the ninth aspect of the invention may, as appropriate, include any or all features described in relation to the first and second aspects of the invention.
According to a tenth aspect of the invention there is provided a method for reducing the volume of silicon needed for a plant growth response, the method comprising applying the composition according to the first aspect of the invention or the formulation according to the second aspect of the invention to the plant or to an environment thereof. The method according to the tenth aspect of the invention may, as appropriate, include any or all features described in relation to the first and second aspects of the invention.
The methods according to the third to tenth aspects of the invention may comprise the step of applying the composition according to the first aspect of the invention or the formulation according to the second aspect of the invention to the plants or an environment thereof at intervals which are greater than two weeks.
The methods according to the third to tenth aspects of the invention may comprise the steps of applying a silicon fertiliser and the composition according to the first aspect of the invention or the formulation according to the second aspect of the invention to the plants or an environment thereof.
By applying the composition or formulation and a silicon-based fertiliser to plants, the silicon-based fertiliser can be applied less frequently and at reduced application rates which enables treatments for improving silicon uptake and distribution to become more cost-effective.
According to an eleventh aspect of the invention there is provided a method for preparing the agricultural composition, the method comprising mixing a water-soluble source of silicon and a silicon transport stimulant comprising an aryl substituted urea in a solvent. The method according to the eleventh aspect of the invention may, as appropriate, include any or all features described in relation to the first aspect of the invention.
In some embodiments the solvent comprises water.
In some embodiments the mixture comprising the water-soluble source of silicon and the silicon transport stimulant may be heated. Suitably the mixture may be heated to a temperature of at least 40° C.
In some embodiments the mixture may be stirred. Suitably the mixture may be stirred at 100-200 rpm.
According to an eleventh aspect of the invention there is provided a use of the composition according to the first aspect of the invention or of the formulation according to the second aspect of the invention as a fertiliser for administration to crops. The use according to the eleventh aspect of the invention may, as appropriate, include any or all features described in relation to the first to tenth aspects of the invention.
According to a twelfth aspect of the invention there is provided the use of an aryl substituted urea as a silicon transport stimulant. The use according to the twelfth aspect of the invention may, as appropriate, include any or all features described in relation to the first to eleventh aspects of the invention.
In some embodiments a phenyl substituted urea may be used as a silicon transport stimulant. Suitably, one or more of DPU, CPPU, NDPU, mono- or di-methyl DPU and mono- or di-ethyl DPU may be used as a silicon transport stimulant.
In order that the invention may be more clearly understood one or more embodiments thereof will now be described, by way of example only, with reference to the accompanying drawings, of which:
The following composition was prepared as described:
Analysis: Silicon (SiO2) 21% w/w, 50 ppm Diphenylurea
The composition was prepared by adding water to a vessel, ensuring that the temperature of the water is at least 40° C. This is then stirred with a mixer to achieve a reasonable vortex (approx 100-200 rpm), upon which the colour is added and the N,N diphenyl urea is added and mixed. Thereafter, Potassium Silicate is added to the vessel, and again, mixing was continued until it had dissolved. Thereafter to the vessel Urea is added and mixed until dissolved. The solution mixed for 30 minutes before packaging.
Using a similar procedure to that described in Example 1, the following composition was prepared:
Analysis: Si 21% w/w, 50 ppm CPPU
The composition was prepared by adding water to a vessel, ensuring that the temperature of the water is at least 40° C. This is then stirred with a mixer to achieve a reasonable vortex (approx 100-200 rpm), upon which the colour is added and the N-(2-Chloro-4-pyridal)-N′-phenylurea is added and mixed. Thereafter, Potassium Silicate is added to the vessel, and again, mixing was continued until it had dissolved. Thereafter to the vessel Urea is added and mixed until dissolved. The solution mixed for 30 minutes before packaging.
Using a similar procedure to that described in Example 1, the following composition was prepared:
Analysis: Silicon (SiO2) 21% w/w, 100 ppm Diphenylurea
Using a similar procedure to that described in Example 2, the following composition was prepared:
Analysis: Silicon (SiO2) 21% w/w, 100 ppm CPPU
Using a similar procedure to that described in Example 1, the following composition was prepared:
Analysis: Silicon (SiO2) 21% w/w, 200 ppm Diphenylurea
Using a similar procedure to that described in Example 2, the following composition was prepared:
Analysis: Silicon (SiO2) 21% w/w, 200 ppm CPPU
Experiments were conducted to evaluate what (if any) difference the application of a formulation based on the present invention made to the growth and speed of development of plants when compared with a formulation blank using standard silicon at the same levels.
Two formulations were used: Formulation 1 (inventive), and the same formulation without a silicon transport stimulant (N,N Diphenylurea) incorporated (formulation blank), and a water only treatment (control). The formulations are shown below:
This study used an experimental design of 6 treatments: 1) Control, 2) Formulation 1 (inventive), 3) Formulation 2 (formulation blank), 4) salt stressed, 5) formulation 1 (inventive) and salt stressed, 6) formulation 2 (formulation blank) and salt stressed. All treatments had 10 replicates.
Seeds of Pak Choi (Brassica Chinensis) were germinated in seed trays and transplanted out after 7 days into 1 litre pots containing 700 grams of Levington M3 growing medium. Plants were grown under lighted and heated greenhouse conditions, for a further three weeks before treatment commenced.
Formulation 1 and formulation 2 treatments were applied at a dose of 0.5 ml per plant daily.
Plants were watered daily, with 50 ml of water in the first two weeks and 100 ml in later growth stages. Control watered plants were watered with fresh water. Salt Stressed plants were watered with 100 mmol salt solution, 5.84 grams ltr-1.
Plants were treated for 1 week, at 1 week the oldest, alive, leaf was harvested and sent to NRM requesting composite values of Ca and Na within the plant tissues.
Plants were treated for a further 2 weeks and then fully harvested (6 weeks after initial seeds were planted), and fresh leaf weight recorded. The tissues were dried out for a further week and dry weight recorded.
Measurements were taken at harvest after four weeks and results are summarised below:
Formulation 2 (a standard potassium silicate) did not significantly increase crop growth (fresh weight) either under normal or saline water regimes, however formulation 1 (inventive) significantly improved growth in crops grown under normal water and also under salinity stress. Salinity significantly reduced growth of untreated plants compared to control, formulation 2 did not significantly improve growth under salinity, however formulation 1 allowed normal growth (not significantly different to control) under saline growing conditions. The experiment demonstrates that the presence of a silicon transport stimulant such as N, N Diphenylurea improves the performance of potassium silicates both for improving plant growth and reducing damage to crop growth from saline water.
Under normal water both formulation 1 and formulation 2 increased growth rate (% head size attained) over the tested period (6 weeks). However formulation 1 gave significantly faster growth as well as a larger plant.
It was demonstrated that addition of a silicon transport stimulant (50 ppm DPU) to a standard potassium silicate stimulated significant growth in pak choi plants, and prevented a decrease in growth under saline growing conditions that the standard silicon did not achieve.
When saline water is introduced pak choi sodium content was significantly increased. Application of a conventional silicon fertilizer (formulation 2) increased sodium uptake in both control and salt conditions. When a silcon transport stimulant was added (treatment 1) significantly less sodium was taken up by plants under both control and saline water than in the formulation blank.
This coincided with better growth and speed of growth over untreated and standard silicon treated plants.
An experiment was set up to evaluate what (if any) difference the application of a formulation containing potassium silicate and a silicon transport stimulant made to the growth and speed of development of onions (Allium fistulosum var Ishikura) when compared with a formulation blank using standard silicon at the same levels, and the silicon transport stimulant alone.
Three formulations were used: Formulation 1(inventive), and the same formulation without a silicon transport stimulant (N,N Diphenylurea) incorporated (formulation blank), a silicon transport stimulant (STS) only treatment and a water only treatment (control). The formulations are shown below:
This study used an experimental design of 4 treatments: 1) Control, 2) Formulation 1 (inventive), 3) Formulation 2 (silicon only), 4) Formulation 3 (STS only). All treatments had 4 replicates.
Seeds of spring onion (Allium fistulosum var ishikura) were germinated in seed trays and transplanted out after 7 days into 5 litre pots containing of Levington M3 growing medium. Plants were grown under lighted and heated greenhouse conditions, for a further three weeks before treatment commenced.
Formulation 1, formulation 2, and formulation 3 treatments were foliar applied as a 1% spray solution to 1 weeks, 2 weeks and 3 weeks and 4 weeks after transplanting.
Plants were watered daily with an equal amount of water per pot as needed. Plants were grown as bunches (same as commercial practice) for 11 weeks then fully harvested and measured for height, diameter, bulb weight and total fresh weight per pot. Speed of growth (stem extension of largest leaf) was monitored throughout the growing period.
Neither silicon or the STS treatments significantly increased stem dimeter, bulb diameter or total fresh weight, whilst the inventive formula increased all tested growth parameters.
The speed of growth tested over a nine day period following application was measured, with no significant increase in growth rate measured with either silicon or STS alone but a significant increase in growth rate using the inventive formula.
These results demonstrate a clear synergy with growth being achieved with a combination of silicon and an STS that is not possible using a standard silicon source.
An experiment was set up to evaluate what (if any) difference the application of a formulation based on the present invention made to the growth and speed of development, flowering and photosynthesis of Pot Marigold (Calendula officinalis var calypso) when compared with a formulation blank using standard silicon at the same levels, and the silicon transport stimulant alone.
Three formulations were used: Formulation 1(inventive), and the same formulation without a silicon transport stimulant (N,N Diphenylurea) incorporated (formulation blank), a silicon transport stimulant (STS) only treatment and a water only treatment (control). The formulations are shown below:
This study used an experimental design of 4 treatments: 1) Control, 2) Formulation 1 (inventive), 3) Formulation 2 (silicon only), 4) Formulation 3 (STS only). All treatments had 7 replicates.
Seeds of Pot Marigold (Calendula officinalis var calypso) were germinated in seed trays and transplanted out after 7 days into 2 litre pots containing peat substitute compost. Plants were grown under lighted and heated greenhouse conditions.
Formulation 1, formulation 2, and formulation 3 treatments were foliar applied as a 1% spray solution to at the three leaf stage, and again after 6 weeks.
Plants were watered daily as needed and were grown for twelve weeks following first treatment before being harvested and measured. Measurements were taken of chlorophyll fluorescence, a key stress indicator, 1 and 6 weeks after first treatment to assess the length of any effect on photosynthesis. Measurements of height, flower and bud number were measured periodically, and final cumulative flower and bud production was recorded. During the study whitefly were present in the glasshouse, and damage to the plant leaves was assessed at harvest.
Standard silicon treatment did not signifcanty effect chlorophyll fluorescence, a key indicator of photosynthesis. The inventive formula significantly increased photosynthesis for a 6 week period following application. The number of flower buds present on the plants was measured 6 weeks after each application. The inventive formula had significantly higher flower bud numbers at both 6 and 12 weeks, whereas neither the silicon nor the STS treatments were different from the control.
After 12 weeks (six weeks after second application) the experiment ended. Final plant height was measured with the inventive formula being the only treatment significantly different to the untreated control, with taller plants. Total flowers produced during the 12 week period studied were recorded, again the inventive formula treatment produced significantly more flowers than than all other treatments.
During the study whitefly were present in the glasshouse, with the control, STS and silicon only treatments characteristic damage to leaves from feeding occurred, whereas plants treated with the inventive formula had no damage (see photograph).
As best shown in
This experiment demonstrates that the use of STS combined with silicon produces improvements in flower production, plant height, photsynthesis and reduced insect damage (whitefly). Neither silicon alone nor the STS treatments had any significant effect alone.
The experiment gives clear evidence of improved metabolic activity and transport of the silicon by use of a combination of an STS and silicon. This is evidenced by effects 6 weeks after application in new plant parts that were not present at the time of application.
The inventive formulation showed protection from pest damage in leaves that had grown after application, which would not be expected with conventional silicon applications and was not observed in the silicon olny or STS only treatments. This is clear evidence of continued mobility, and better transport.
Metabolic activity (photosynthesis) was increased 6 weeks after application in leaves that had grown after application. Silicon is not capable of being translocated 6 weeks after application as it quickly gets made immobile as polymers in the leaf rendering it metabolically inactive and immobile. The inventive formula was able to produce higher photosythesis in new leaves 6 weeks after application, whereas the other formulations had no effect.
These results demonstrate a clear synergy with growth being achieved with a combination of silicon and an STS that is not possible using a standard silicon source.
The inventive formulation produced more flowers, more buds, taller plants, higher photosynthesis and improved insect resistance when compared to conventional silicon application.
An experiment was set up to evaluate what (if any) difference the application of a formulation based on the present invention made to the growth and speed of development of basil (Ocimum x africanum) when compared with a formulation blank using standard silicon at the same levels.
Two formulations were used: Formulation 1(Inventive), and the same formulation without a silicon transport stimulant (N-(2-Chloro-4-pyridal)-N′-phenylurea) incorporated (formulation blank). The formulations are shown below:
Formulation 1 (Inventive) Formulation 2 (Silicon Only)
This study used an experimental design of 3 treatments: 1) Silicon only (Formulation 2), 2) Inventive (Formulation 1), 3) half inventive (Formulation 1 at half rate). All treatments had 10 replicates.
Seeds of lemon basil (Ocimum x africanum) were germinated in vermiculite pots and grown suspended in water in a hydroponic growing system lit by LED lighting. Each treatment was added to the water at 0.1 mm per plant per week for three weeks. Plants were grown for 4 weeks and then harvested and measured.
At harvest total fresh weight of shoots, plant height, and branching were measured to assess the effect of treatments on growth and architecture of the plants.
Plants treated with the silicon only formulation blank (T1) had significantly lower height, shoot weight, primary internodes and lateral branches than the inventive formula at the same rate. At half the level applied (T3) the inventive formula significantly increased height, fresh weight and lateral branching when compared to the silicon only treatment.
The presence of an STS in the formulation allows silicon to be more effective at stimulating plant growth even when applied at half the level allowing lower application rates of silicon to be effective.
Thye use of STS in silicon formulations significantly increases plant height, branching and total shoot biomass in plants.
Basil is a low silicon accumulator, and accumulates silicon at a lower rate than is available in water. This experiment demonstrates that use of STS stimulates better uptake of silicon in plants, leading to increased plant growth.
In low silicon accumulation plants (all dicots) the amount of silicon available is not a limiting factor growth, rather it is the inability of the plant to take it up in roots and transport it to shoots via xyllem. By adding a silicon transport stimulant (STS) significant growth improvements can be obtained even when supplying half the level of silicon. This clearly demonstrates that use of an STS in silicon improves the plants ability to take up silicon and use it for growth.
An experiment was set up to evaluate what (if any) difference the application of a formulation based on the present invention made to the growth and speed of development of parsley (Petroselinum crispum var VPA30) when compared with a formulation blank using standard silicon at the same levels, and the silicon transport stimulant alone.
Three formulations were used: Formulation 1(inventive), and the same formulation without a silicon transport stimulant (N,N Diphenylurea) incorporated (formulation blank), a silicon transport stimulant (STS) only treatment and a water only treatment (control). The formulations are shown below:
Formulation 1 (Inventive) Formulation 2 (Silicon Only)
Formulation 3 (STS Only)
This study used an experimental design of 4 treatments: 1) Control, 2) Formulation 1 (inventive), 3) Formulation 2 (silicon only), 4) Formulation 3 (STS only). All treatments had 11 replicates.
Seeds of parsley (Petroselinum crispum var VPA30) were germinated in seed trays and transplanted out (when the first true leaf formed) into 2 litre pots containing of peat free compost. Plants were grown under lighted and heated greenhouse conditions, for a further four weeks before treatment commenced.
Formulation 1, formulation 2, and formulation 3 treatments were soil applied at an equivalent rate to 1 L/Ha at week 0 and grown for 14 days.
Plants were watered daily with an equal amount of water per pot as needed. Shoot number, diameter and growth rate were assessed and at harvest samples were retained and kept in refrigerated storage for 14 days then assessed for quality (visual score 1=rotted, 2=yellowed, 3=light green, 4=medium green, 5=dark green) to assess impact on post-harvest shelf life.
Shoot number 3, 7 and 14 days after treatment.
The inventive formulation was the only treatment to significantly increase shoot number over control 3 days after treatment (faster acting). The inventive treatment significantly increased shoot number over the standard silicon treatment at 3, 7 and 14 days.
Growth Rate (48 h Increase in Shoot Number and Length) 3 Days after Treatment.
3 days after treatment the silicon only treatment did not significantly affect the rate of new shoot production (48 h shoot number increase), whilst the STS alone treatment significantly reduced the rate of new shoot production. The inventive formula (silicon combined with STS) gave a large and significant increase in the rate of new shoot production.
3 days after treatment the speed of shoot extension of the youngest emerging shoot was not significantly increased with the silicon only treatment and was significantly reduced when treating using STS alone. The inventive formula (silicon plus STS) showed a large and significant increase in speed of shoot growth.
Growth Rate (4 Day Increase in Shoot Number and Length) 7 Days after Treatment.
7 days after treatment the silicon only treatment had significantly increased the rate of shoot production relative to the control (4 day shoot number increase), but the inventive formula (silicon plus STS) gave a large and significant increase over both the control and silicon only treatment in terms of shoot production. The STS only treatment had no significant effect over the control on shoot production.
7 days after treatment neither the silicon only treatment nor the STS only treatment had any significant effect on the speed of shoot extension (4 day increase in emerging shoot length over that of the untreated control. The inventive formula gave a significant increase in the speed of shoot extension relative to the control, the silicon only treatment and the STS only treatments.
Leaf colour was used as a shelf life indicator (visual score 1=rotted, 2=yellowed, 3=light green, 4=medium green, 5=dark green). 4 is the threshold at which the parsley would saleable, <4 it would be discarded as not fit for use.
7 days post harvest, parsley that had been treated with silicon only and the inventive formula were significantly greener than the parsley which was subjected to the control and STS only treatments. By 14 days after treatment the parsley which has been treated with the inventive was significantly greener than the parsley that has been treated with the silicon only formula, and the only one to achieve the top score. At 20 days post harvest, parsley which had been treated with the control, silicon only and STS only formulas were all below the saleable threshold of 4, but the inventive formula was significantly better and above the threshold.
Parsley is a dicotyledon, and is known to be a poor transporter of silicon. This experiment applied silicon to the soil at a low rate and the experiment clearly shows significantly faster response both in terms of new shoot production and speed of shoot growth. After 3 days the inventive formula was significantly improving growth whereas silicon alone had no significant effect. This is a clear indication that the plant was better able to take up silicon when the STS was incorporated.
It took 7 days for silicon alone to have any significant impact on growth, but at all stages the inventive formula gave stronger growth in terms of shoot number, growth rate and height. This is indicative of continued improved uptake, transport and distribution over the course of the experiment.
The inventive formula allows small levels of silicon to be biologically active via better uptake, transport and distribution through the plant. It demonstrates that lack of mobility rather than dose rate of silicon is the limiting factor in growth rate, not the amount of silicon added to the soil.
The post harvest storage measurements show that the inventive step allows silicon to be used to prolong shelf life in plants, with the inventive formula extending storage life longer than silicon alone. This is a clear indication of improved uptake and more even distribution through the plant.
The one or more embodiments are described above by way of example only. Many variations are possible without departing from the scope of protection afforded by the appended claims.
| Number | Date | Country | Kind |
|---|---|---|---|
| 2102806.3 | Feb 2021 | GB | national |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/GB2022/050503 | 2/24/2022 | WO |
