ALCOHOL SOLUBLE PROTEIN GAS-LIQUID INTERFACE SELF-ASSEMBLED POROUS MEMBRANE AND PREPARATION METHOD THEREOF

Abstract

An alcohol soluble protein gas-liquid interface self-assembled porous membrane and a preparation method thereof are provided. The alcohol soluble protein gas-liquid interface self-assembled porous membrane is prepared from an alcohol soluble protein membrane storage solution by an anti-solvent method. The porous interface membrane is rapidly prepared by an alcohol soluble protein interface self-assembled one-step method and can be rapidly formed within 4 seconds, which greatly improves the preparation efficiency of the alcohol soluble protein membrane. The structure (size, pore diameter, micro porosity) of the alcohol soluble protein interface self-assembled porous membrane is precisely regulated and controlled by regulating and controlling process parameters, and a new preparation solution of an alcohol soluble protein base membrane, which is more efficient and has a modifiable structure compared with an alcohol soluble protein membrane prepared by a traditional solvent evaporation method, is developed.

Description

Claims

1. A method for preparing an alcohol soluble protein gas-liquid interface self-assembled porous membrane, comprising the following steps: S1 adding an alcohol soluble protein into a good solvent to be dissolved to obtain a membrane storage solution with an alcohol soluble protein concentration of 10 to 30 wt%;S2 regulating and controlling a temperature of an anti-solvent to be 25° C. to 40° C.; andS3 dropping the membrane storage solution in the S1 into the anti-solvent in the S2 to form a self-assembled porous membrane;wherein pH of the anti-solvent is 3.0 to 10.0, and an ionic strength thereof is 0 to 700 mM;wherein the good solvent comprises any one of 50% to 90% (v/v) of the aqueous ethanol solution, 60% to 90% (v/v) of the aqueous methanol solution, 60% to 100% (v/v) of the aqueous acetic acid solution and 70% to 90% (v/v) of the aqueous acetone solution;wherein the anti-solvent comprises any one of water, 0.1% to 50% (v/v) of the aqueous ethanol solution, 0.1% to 60% (v/v) of the aqueous methanol solution, 0.1% to 60% (v/v) of the aqueous acetic acid solution and 0.1% to 70% (v/v) of the aqueous acetone solution;titration of the anti-solvent in the S3 is single titration, and a volume ratio of the membrane storage solution to the anti-solvent during titration is 1:(500 to 10,000).

2. (canceled)

3. The method according to claim 1, wherein the alcohol soluble protein comprises any one of gliadin, zein, kafirin and hordein.

4-8. (canceled)

9. An alcohol soluble protein gas-liquid interface self-assembled porous membrane prepared by the method of claim 1.

10. A use of the alcohol soluble protein gas-liquid interface self-assembled porous membrane of claim 9 in preparation of an active substance controlled release product.

11. An alcohol soluble protein gas-liquid interface self-assembled porous membrane prepared by the method of claim 3.