The present invention relates generally to Photosynthetic organisms, such as Algae and micro-algae cultivation systems, and more particularly to photobioreactor (PBR) Algae cultivation systems.
Algae are a group of relatively simple, plant like organisms, most of which are capable of performing photosynthesis: They capture light and use its energy to convert CO2 into sugars and oxygen. In this way, they largely contribute to the global oxygen production (between 50 to 87 percent). There are 80,000 to 100,000 different algae species with widely varying characteristics. Algae size ranges from micrometers of unicellular micro-algae to macro-algae seaweeds of tens of meters. Globally, there is growing interest in algae as production organisms. Algae contain lipids (oil), proteins and carbohydrates (sugars), and especially marine algae have been used as food and fertilizers for centuries. Commercial farming of algae has a long history. Commercial large-scale cultures of the micro-algae species have started in the early 1960s. Nowadays, approximately 200 species of micro-algae are used worldwide. There is a well-established global market for algae-based food and feed products, but microalgae have other functions. More recently, algae are used for the production of ethanol (fermentation) or biodiesel (conversion), and research using GM algae for the production of pharmaceuticals is currently on-going.
Algae in general and single cell micro-algae in particular, represent an important layer of the ecological system, both by their prominent function in the food chain and in absorption of carbon dioxide from the atmosphere and oxygen release.
The Algae chemical composition is characterized in the abundance of poly-unsaturated fatty acids (PUFA), unique proteins and toxins, a variety of polysaccharides and pigments. Single cell Algae grow and multiply at a faster rate than other plants (20-30 times faster).
Currently, systems for commercial cultivation of algae can be divided into three types:
1. Open systems, such as raceways and ponds
2. Closed systems, such as photo-bio-reactors (PBR)
3. Semi-closed systems, such as poly-ethylene sleeves.
Until the year 1940, the growth of micro-Algae had been done only by scientists and in labs. During the end of the 1940's, experimentation had started with micro-Algae growth outside labs, as a branch of advanced agriculture, and as food for humans and feeding animals. An additional field of interest focuses on the benefit derived from their ability to exchange gasses and produce Oxygen, and lower the emission of Carbon Dioxide.
Open artificial systems for Algae growth, as opposed to natural water sources, are usually built out of asphalt, concrete, plastic sheets, rubber or foam, so that cleaning can be done efficiently. The system may be shaped as a pool, container or elliptical track that the Algae culture is swirled in by air blowing or by a mixing paddle.
The disadvantages of the open systems have led to the development of closed Photo Bio Reactors (PBR). In Europe of the 1970's, both closed and open systems were used to grow micro-Algae for the production of Methane. It was then found that cultivation in closed systems is essential in producing high-valued products. The designing of PBR's which achieve high production ability on the one hand, while reducing the production costs, on the other hand, is not trivial. There are many different types of PBR's.
For small-scale cultivation of micro-Algae, hybrid systems are used which can be described as semi-closed systems. These are containers for disposable use such as, sleeves, panels, baths or plastic sheets, and are exposed to the external surroundings. These systems are usually used with artificial lighting in a clean room, or exposed to sunlight but protected from the environment, such as in a greenhouse.
When cultivation methods in the existing Hat Panels Systems are examined, a number of obstacles are presented that need to be overcome in order to maximize the system operation for commercial use. Among the obstacles are:
1. Setting-up and operating expenses
2. System cleaning
3. Up-scaling ability
4. Dismantling and mobility
The growth system of the present invention, it being a closed-flat panels system, overcomes the above obstacles, by possessing the advantages of both closed and open systems.
Accordingly, it is a principal object of the present invention to overcome the limitations of prior art Algae growth systems by providing an Algae growth system which combines the advantages of closed systems with the simplicity and low costs of open systems, said system comprising:
a transparent bioreactor device comprising:
and a blowing means for providing carbon dioxide enriched air,
wherein said blowing means provides air flow through a medium in said bioreactor device, for developing a vortex in the medium containing the algae, thereby preventing sinking of algae and causing them to be uniformly exposed to light.
According to a preferred embodiment of the present invention, the photo bioreactor (PBR) device comprises a transparent aquarium with longitudinal flat side walls, a curved base, rounded end walls without corners and a lid that can be opened. The PBR device is filled with a cultivation medium and is vortexed by air flowing through a tube having holes, in the bottom of the aquarium. The aquarium is constructed in such a way that the internal side is smooth and there are no corners, edges, or rough surfaces that elevate friction and are prone to adherence of biofilm or contaminating aggregates, which are usually very difficult to clean and sterilize. By having a rounded and smooth construction, contamination is avoided.
The PBR device base is composed of a rigid material that is curved according to the varying PBR device width. The curved base results in a uniform water flow in the entire width of the device, which prevents algae from settling on the bottom and on the walls of the PBR device. Furthermore, it allows for a large width of the PBR device to remain without algae settling or sinking therein, and there is no need for a lot of air for appropriate movement of the algae. The rounded base further allows easy cleaning and draining.
A drain placed on the other side of the base allows for fast harvesting which provides smaller chances of the Algae to decompose or rot.
For the purpose of continuous growth, when only part of the Algae are harvested and the rest remain in the PBR device, and growth medium is added for continued growth, the drain is especially useful. During continuous growth, after a while the Algae begin to grow on the walls, then a wiper can be wiped against the walls and remove the Algae from them. This can be repeated frequently when the Algae begin growing on the walls again.
The device is modular which provides a significant advantage in commercial cultivation.
In accordance with a further embodiment of the present invention, there is provided a PBR device with an air tube placed externally to the PBR device. The external air tube has a laterally extending slit, resulting in a U-shaped tube. Slit air tube is attached on its opened end to the curved base. The curved base has holes created thereon, to allow the CO2 blown from within the air tube to enter the PBR device. This construction minimizes accumulation of dirt, and is easy to clean. In addition, there is no limit to what the length of the tube can be, and it eliminates problems existing with the internal air tube, such as the tube becoming lifted and having to glue the tube to the base, which causes contamination.
In accordance with yet a further embodiment of the present invention, there is provided a method for external temperature control by using a wet cloth blanket, or an equivalent, when the blanket is soaked in water at a desirable temperature, thus heat transfer is created between the wet blanket and the PBR device growth medium.
In accordance with an additional embodiment of the present invention, there is provided another method for external temperature control by using a double-jacket, which is double-walled with water or cooling liquid directed to flow therethrough at a desirable temperature. The double-jacket is wrapped around the PBR device, either on both sides or only on one side. The double jacket creates heat transfer between the cooling liquid in it and the liquid inside the PBR device. The double jacket may be transparent or opaque.
In accordance with further embodiments of the present invention, there are provided yet other methods for external temperature control by soaking the system in a pond of water, outer water sprinklers, an inner heat exchange coil flowing with temperature-controlled water, placed within the PBR device, and a plastic tent covering the entire PBR system having air conditioning to control the temperature. Inner heat exchange coil may be removed from the PBR device to be cleaned and sterilized.
In accordance with yet another further embodiment of the present invention, there is provided a sun tracking system on the PBR device, which causes the PBR devices to tilt towards the sun, thereby increasing the exposure to the sunlight.
By connecting several devices to one another into one growth unit, more benefits are provided, such as lowering the operational expenses.
The system is created by connecting several devices to one another at either end of the device via a hose positioned perpendicular to the bottom of the device. Alternatively, the devices may be connected to each other by placing a water-filled connector aquarium perpendicular to the devices. Each device is connected to the connector aquarium via openings in the connector aquarium, which creates a manifold on one side or both.
The device can work anywhere in the world, and can be used with natural or artificial light, such as, but not only, Fluorescence and LED.
Additional features and advantages will become apparent from the following drawings and description.
For a better understanding of the invention with regard to the embodiments thereof, reference is made to the accompanying drawings, in which like numerals designate corresponding elements or sections throughout, and in which:
It is a principal object of the present invention to provide a modular Flat Panel Photo-Bioreactor system for cultivating many different types of algae, which provides a significant advantage in commercial cultivation.
Referring now to
Longitudinal flat side walls 40a-b are made of rigid transparent plastic with a thickness in a range of 0.5-12 mm, a height in a range of 30-300 cm or more, width in a range of 3-40 cm and a length in a range of 0.5-1000 m. The walls may be constructed of any transparent material, such as glass or plastic.
PBR device 30 is closely surrounded by frame 50 which maintains PBR device 30 in its position, and further prevents walls 40 from becoming distorted due to the positive hydro-pressure created in PBR device 30. Walls 40 are plastic which has elasticity that causes walls 40 to be prone for distortion.
On end wall 44 there is formed a rounded closure laterally extending to base 42 without angles. Air tube 46 extends through one end wall 44 situated at a height of 0.5-5 cm from the bottom, running all along the length of PBR device 30. Tube 46, having a diameter of 16-60 mm, has holes in the size of 0.5-5 mm, 1-20 cm apart from each other, directed upwards in order to blow Carbon Dioxide-rich air into the medium for the growth of the Algae, and also for causing vortexing motion of the water to avoid algae sinking, flocculation and aggregation, and for providing uniform exposure of light to all the algae. Walls 44 also allow for harvesting algae within the device 30 and high quality cleaning and disinfecting the device 30.
For economizing purposes air tube 46 may blow air that is not enriched with CO2 into PBR device 30. In this case, CO2 is delivered into PBR device 30 via a separate tube inserted through a separate opening in PBR device 30, or by placing in PBR device 30 a ceramic stone, or other Carbon sources such as, but not only, bicarbonate, glycerol or sugar.
A lid 49 is placed on the top of PBR device 30 to provide a sterile environment by preventing contaminants from entering PBR device 30 by creating a positive hydro-pressure in the PBR device 30. Lid 49 is composed of rigid cleanable material which is transparent to allow light to pass through it. Lid 49 also minimizes the CO2 intake, allows for working with an open or closed device, contributes to temperature homeostasis according to weather, allows for an option for different types of cooling and allows easy cleaning and a possibility for automation. There is less medium evaporation, no medium spraying and rain cannot enter the device 30.
The PBR device 30 walls are corner-less and made of rigid materials so that the walls are smooth without creases, folds, or “dead” areas, a feature which provides a large surface-area that provides a large light exposure. Due to these features, there is substantially no Algae growth on the walls which are easily cleaned and sterilized.
Air tube 46 is connected to one end of a blower which is connected on its other end to a CO2 tank (shown in
Referring now to
Referring now to
Referring now to
Referring now to
Referring now to
Referring now to
Lid 49 may be constructed of any material such as rigid plastic, soft plastic (such as a bag), or any other suitable material, and can be either transparent or opaque. Lid 49 may be attached to PBR device 30 by hinges, or by simply placing lid 49 on PBR device 30, or by any other suitable configuration.
Referring now to
Referring now to
Referring now to
There is provided a water pump 60, which is a source of water for spray system 90 situated above the plurality of PBR devices 30 (not shown). Spray system 90 has multiple spray nozzles 92 positioned between each device 30, for the purpose of cooling the water in PBR devices 30. Spray system 90 may also function as the source of water for filling a double jacket, and moistening a blanket, both for the purpose of cooling the water in PBR device 30.
The same system 200 can be used for heating as well for heat mass transfer, by filling the system with hot/warm water instead of cold water.
Referring now to
Although particular embodiments of the invention have been described and illustrated herein, it is recognized that modifications and variations may readily occur to those skilled in the art and consequently, it is intended that the claims be interpreted to cover such modifications and equivalents.
Filing Document | Filing Date | Country | Kind |
---|---|---|---|
PCT/IL2014/050900 | 10/14/2014 | WO | 00 |
Number | Date | Country | |
---|---|---|---|
61890331 | Oct 2013 | US |