Claims
- 1. A biologically pure culture of a microorganism producing a highly alkaline protease, wherein said microorganism is a microorganism transformed to contain inexpressible form a highly alkaline protease gene isolated from Bacillus pumilus DSM 5777, said protease having either set of the following properties:
- (1) Action: degradation of proteins and peptides;
- (2) pH optimum: approximately between pH values of 10.5 to 11.0;
- (3) pH stability: upon incubation at 4.degree. C. for 24 hours at pH values between 9.7 to 10.8, the enzyme proves to be completely stable;
- (4) temperature optimum: approximately 60 .degree. C.;
- (5) temperature stability: upon incubation of the protease at temperatures of up to 45.degree. C. for 15 minutes, the activity of the protease is substantially 100%; after 15 minutes' incubation at 50.degree. C., the residual activity of the protease is at least 90% or
- (1) Action: degradation of proteins and peptides,
- (2) pH optimum: approximately between pH values of 8.5 to 9.0;
- (3) pH stability: upon incubation at 4.degree. C. for 24 hours between pH values of 5.5 to 10.5, the enzyme proves to be completely stable;
- (4) temperature optimum: approximately 50.degree. C.;
- (5) temperature stability: upon incubation of the protease at temperatures of up to 40.degree. C. for 15 minutes, the activity of the protease is substantially 100%; after 15 minutes' incubation at 45.degree. C., the residual activity of the protease is at least 95%.
- 2. A microorganism according to claim 1, wherein said microorganism transformed to contain in expressible form a highly alkaline protease gene isolated from Bacillus pumilus DSM 5777 is selected from the group consisting of Bacillus subtilis, Bacillus alcalophilus, Bacillus licheniformis, and Bacillus amyloliquefaciens.
- 3. A microorganism according to claim 2, wherein said microorganism contains the genetic information for said protease in an expression vector.
- 4. A microorganism according to claim 3, wherein said expression vector is a plasmid isolated from a microorganism selected from the group consisting of DSM 6879 and DSM 6880.
- 5. A microorganism according to claim 1, wherein said microorganism is selected from the group consisting of microorganism DSM 6879 and microorganism DSM 6880.
Priority Claims (1)
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Date |
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42 18 448.7 |
Jun 1992 |
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Parent Case Info
This application is a division of application Ser. No. 08/067,086, filed May 26, 1993, now U.S. Pat. No. 5,346,822.
Foreign Referenced Citations (1)
Number |
Date |
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4111321 |
Oct 1991 |
DEX |
Non-Patent Literature Citations (3)
Entry |
Anonymous Research Disclosure (England) #20750, pp. 285-287, 1981. |
Fabian, Folia Microbiologica 15:169-175 (1970). |
Qiu et al., Acta Microbiologica Sinica 30:445-449 (1990). |
Divisions (1)
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Number |
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Parent |
67086 |
May 1993 |
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