Patent Application
20080287492
This invention relates to alkylpyridyl quinoline derivatives, pharmaceutical compositions comprising them, and the use of such compounds in the treatment of central nervous system and peripheral diseases or disorders. This invention also relates to the use of such compounds in combination with one or more other CNS agents to potentiate the effects of the other CNS agents. The compounds of this invention are also useful as probes for the localization of cell surface receptors.
Tachykinin receptors are the targets of a family of structurally related peptides which include substance P(SP), neurokinin A (NKA) and neurokinin B (NKB), collectively “tachykinins.” Tachykinins are synthesized in the central nervous system (CNS), and peripheral tissues, where they exert a variety of biological activities. Three tachykinin receptors are known which are named neurokinin-1 (NK-1), neurokinin-2 (NK-2) and neurokinin-3 (NK-3) receptors. NK-1 and NK-2 receptors are expressed in a wide variety of peripheral tissues and NK-1 receptors are also expressed in the CNS whereas NK-3 receptors are primarily expressed in the CNS.
The neurokinin receptors mediate a variety of tachykinin-stimulated biological effects that include: transmission of excitatory neuronal signals in the CNS and periphery (e.g. pain signals), modulation of smooth muscle contractile activity, modulation of immune and inflammatory responses, induction of hypotensive effects via dilation of the peripheral vasculature, and stimulation of endocrine and exocrine gland secretions.
In the CNS, activation of NK-3 receptors has been shown to modulate dopamine, acetylcholine and serotonin release, suggesting a therapeutic utility for NK-3 ligands for the treatment of a variety of disorders including anxiety, depression, schizophrenia and obesity. Studies in primate brain have shown the presence of NK-3 mRNA in a variety of regions relevant to these disorders. Studies in rats have shown NK-3 receptors to be located on MCH-containing neurons in the lateral hypothalamus and zona incerta, again suggesting a therapeutic utility for NK-3 ligands for obesity.
Non-peptide ligands have been developed for each of the tachykinin receptors, however known non-peptide NK-3 receptor antagonists suffer from a number of problems such as species selectivity which limits the potential to evaluate these compounds in many appropriate disease models. New non-peptide NK-3 receptor ligands are therefore desirable for use as therapeutic agents and as tools to investigate the biological consequences of NK-3 receptor modulation.
Disclosed are compounds, particularly quinoline derivatives with affinity for NK-3 receptors (NK-3r). These compounds have potential for the treatment of a broad array of diseases, disorders and conditions including but not limited to depression, anxiety, schizophrenia, cognitive disorders, psychoses, obesity, inflammatory diseases including irritable bowel syndrome and inflammatory bowel disorder, emesis, pre-eclampsia, chronic obstructive pulmonary disease, disorders associated with excessive gonadotrophins and/or androgens including dysmenorrhea, benign prostatic hyperplasia, prostatic cancer, and testicular cancer in which modulation of the activity of NK-3 receptors is beneficial.
Ligands for NK-3 receptors disclosed and stereoisomers, enantiomers, in vivo—hydrolysable precursors and pharmaceutically-acceptable salts thereof are compounds of Formula I,
wherein:
R1 is selected from H, C1-4alkyl-, C3-6cycloalkyl- and C1-4alkylOC(O)—;
A is phenyl or C3-7cycloalkyl-;
R2 at each occurrence is independently selected from H, —OH, —NH2, —CN, halogen, C1-6alkyl-, C3-7cycloalkyl-, C1-6alkoxy- and C1-6alkoxyC1-6alkyl-;
n is 1, 2 or 3;
R3 at each occurrence is independently selected from H, —OH, —NH2, —NO2, —CN, halogen, C1-6alkyl-, C1-6alkoxy- and C1-6alkoxyC1-6alkyl-;
m is 1, 2 or 3;
R4 is —(CH2)p-Ar1, wherein p is selected from 1, 2, 3, 4, 5 or 6 and Ar1 is pyridyl;
R5 at each occurrence is independently selected from H, —OH, —CN, halogen, —R6, —OR6, —NR6R7, —SR6, —SOR6 and —SO2R6;
q is 1, 2 or 3;
wherein:
R6 and R7 at each occurrence are independently selected from H, a C1-6 straight or branched alkyl group, a C2-6 straight or branched alkenyl or alkynyl group and a C3-7-carbocyclic group having zero, one or two double- or triple-bonds, wherein said groups are either unsubstituted or substituted with one or more moieties selected from —OH, ═O, —NH2, —CN, halogen, aryl and C1-3alkoxy-;
and,
when R1, R2 or R3 is an alkyl, cycloalkyl, alkoxy or alkoxyalkyl moiety, said moieties are unsubstituted or have 1, 2, 3, 4 or 5 substituents independently selected at each occurrence from —OH, —NH2, —CN, phenyl and halogen.
Also disclosed are pharmaceutical compositions and formulations containing the compounds, methods of using them to treat diseases and conditions either alone or in combination with other therapeutically-active compounds or substances, processes and intermediates used to prepare them, uses of them as medicaments, uses of them in the manufacture of medicaments and uses of them for diagnostic and analytic purposes. In particular are disclosed compounds, compositions containing them, and methods using them for treating or preventing conditions and disorders associated with a wide range of diseases or disorders in which NK-3 receptors are considered to have a role.
Compounds of the invention are compounds of Formula I.
wherein:
R1 is selected from H, C1-4alkyl-, C3-6cycloalkyl- and C1-4alkylOC(O)—;
A is phenyl or C3-7cycloalkyl-;
R2 at each occurrence is independently selected from H, —OH, —NH2, —CN, halogen, C1-6alkyl-, C3-7cycloalkyl-, C1-6alkoxy- and C1-6alkoxyC1-6alkyl-;
n is 1, 2 or 3;
R3 at each occurrence is independently selected from H, —OH, —NH2, —NO2, —CN, halogen, C1-6alkyl-, C1-6alkoxy- and C1-6alkoxyC1-6alkyl-;
m is 1, 2 or 3;
R4 is —(CH2)p-Ar1, wherein p is selected from 1, 2, 3, 4, 5 or 6 and Ar1 is pyridyl;
R5 at each occurrence is independently selected from H, —OH, —CN, halogen, —R6, —OR6, —NR6R7, —SR6, —SOR6 and —SO2R6;
q is 1, 2 or 3;
wherein:
R6 and R7 at each occurrence are independently selected from H, a C1-6 straight or branched alkyl group, a C2-6 straight or branched alkenyl or alkynyl group and a C3-7carbocyclic group having zero, one or two double- or triple-bonds, wherein said groups are either unsubstituted or substituted with one or more moieties selected from —OH, ═O, —NH2, —CN, halogen, aryl and C1-3alkoxy-;
and,
when R1, R2 or R3 is an alkyl, cycloalkyl, alkoxy or alkoxyalkyl moiety, said moieties are unsubstituted or have 1, 2, 3, 4 or 5 substituents independently selected at each occurrence from —OH, —NH2, —CN, phenyl and halogen;
stereoisomers, enantiomers, in vivo-hydrolysable precursors and pharmaceutically-acceptable salts thereof.
Particular compounds are those wherein:
A is phenyl;
R1 is selected from C1-4alkyl-, C3-6cycloalkyl- and C1-4alkylOC(O)—;
R2 is selected from H, halogen and unsubstituted C1-6alkoxy-;
R3 is H or halogen;
n and m are both 1, and
when R1 is an alkyl or cycloalkyl moiety, said moiety is unsubstituted or has 1, 2, 3, 4 or 5 substituents independently selected at each occurrence from —OH, —NH2, —CN and halogen;
stereoisomers, enantiomers, in vivo-hydrolysable precursors and pharmaceutically-acceptable salts thereof.
Other particular compounds are those wherein:
A is phenyl;
R1 is selected from C1-4alkyl- and C3-6cycloalkyl-;
R2 is selected from H, halogen and unsubstituted C1-6alkoxy-;
R3 is H or halogen;
n and m are both 1;
R4 is selected from pyrid-4-yl, pyrid-3-yl and pyrid-2-yl, and
R5 is H;
stereoisomers, enantiomers, in vivo-hydrolysable precursors and pharmaceutically-acceptable salts thereof.
Still other particular compounds are those wherein:
A is phenyl;
R1 is ethyl or cyclopropyl;
R2 is selected from H, F and —OCH3;
R3 is H or F;
n, m, p and q are each 1;
R4 is selected from pyrid-4-yl, pyrid-3-yl and pyrid-2-yl, and
R5 at each occurrence is independently selected from H, —OH and halogen;
stereoisomers, enantiomers, in vivo-hydrolysable precursors and pharmaceutically-acceptable salts thereof.
Yet other particular compounds of the invention are those in accord with Formula II:
wherein R1, A, R2, n, R3, m, R4, R5 and q are as defined for Formula I,
stereoisomers, in vivo-hydrolysable precursors and pharmaceutically-acceptable salts thereof.
Particular compounds are selected from:
stereoisomers, enantiomers, in vivo-hydrolysable precursors and pharmaceutically-acceptable salts thereof.
Compounds of the present invention have the advantage that they may be more soluble, be more easily absorbed and more efficacious in vivo, produce fewer side effects, be less toxic, be more potent, more selective, be longer acting, be less metabolized and/or have a better pharmacokinetic profile than, or have other useful pharmacological or physicochemical properties over known compounds. Using assays for functional activity described herein, compounds of the invention will be found to have IC50's of less than about 1 μM for NK-3 receptors and many compounds will be found to have IC50's of less than about 100 nM for such receptors.
As used herein, unless otherwise indicated, C1-6alkyl includes but is not limited to methyl, ethyl, n-propyl, n-butyl, i-propyl, i-butyl, t-butyl, s-butyl moieties, whether alone or part of another group and alkyl groups may be straight-chained or branched.
As used herein, unless otherwise indicated, C1-6alkoxy includes but is not limited to —O-methyl, —O-ethyl, —O-n-propyl, —O-n-butyl, —O-i-propyl, —O-i-butyl, —O-t-butyl, —O-s-butyl moieties, whether alone or part of another group and alkoxy groups may be straight-chained or branched.
As used herein C3-6cycloalkyl groups include but are not limited to the cyclic alkyl moieties cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl.
As used herein, unless otherwise indicated, C2-6alkenyl includes but is not limited to 1-propenyl, 2-propenyl, 1-butenyl, 2-butenyl and 3-butenyl.
As used herein, unless otherwise indicated, C2-6alkynyl includes but is not limited to ethynyl, 1-propynyl, 2-propynyl, 1-butynyl, 2-butynyl and 3-butynyl.
As used herein, unless otherwise indicated, halo or halogen refers to fluorine, chlorine, bromine, or iodine;
As used herein, aryl includes to phenyl and naphthyl;
As used herein, aromatic or non-aromatic heterocyclic rings include but are not limited to N- or C-linked furyl, imidazolyl, oxazolyl, pyrrolidinyl, thiazolyl, thiophenyl, pyrrolyl, morpholinyl, piperidinyl, piperazinyl, pyrazinyl, pyridyl, pyrimidinyl, indanyl, indolyl, quinolinyl, isoquinolinyl, quinazolinyl, quinoxalinyl, benzo[b]thiophenyl, benzoxazolyl, or benzthiazolyl;
DCM refers to dichloromethane;
EtOAc refers to ethyl acetate;
EDC refers to 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide;
EDTA refers to ethylenediaminetetraacetic acid;
HEPES refers to 4-(2-hydroxyethyl)-1-piperazine ethane sulfonic acid, monosodium salt, and
TEA refers to triethylamine.
In processes described herein, where necessary, hydroxy, amino, or other reactive groups may be protected using a protecting group as described in the standard text “Protecting groups in Organic Synthesis”, 3rd Edition (1999) by Greene and Wuts.
Unless otherwise stated, reactions are conducted under an inert atmosphere, preferably under a nitrogen atmosphere and are usually conducted at a pressure of about one to about three atmospheres, preferably at ambient pressure (about one atmosphere).
The compounds of the invention and intermediates may be isolated from their reaction mixtures by standard techniques.
Acid addition salts of the compounds of Formula I which may be mentioned include salts of mineral acids, for example the hydrochloride and hydrobromide salts; and salts formed with organic acids such as formate, acetate, maleate, benzoate, tartrate, and fumarate salts.
Acid addition salts of compounds of Formula I may be formed by reacting the free base or a salt, enantiomer or protected derivative thereof, with one or more equivalents of the appropriate acid. The reaction may be carried out in a solvent or medium in which the salt is insoluble or in a solvent in which the salt is soluble, e.g., water, dioxane, ethanol, tetrahydrofuran or diethyl ether, or a mixture of solvents, which may be removed in vacuum or by freeze drying. The reaction may be a metathetical process or it may be carried out on an ion exchange resin.
Certain compounds of Formula I may exist in tautomeric or enantiomeric forms, all of which are included within the scope of the invention. The various optical isomers may be isolated by separation of a racemic mixture of the compounds using conventional techniques, e.g. fractional crystallization, or chiral HPLC. Alternatively the individual enantiomers may be made by reaction of the appropriate optically active starting materials under reaction conditions which will not cause racemization.
Compounds of Formula 1 may be prepared by a general method as follows, reacting a pyridinyl-alkanol with an oxidizing agent such as Jones reagent to afford a carboxyalkylpyridine; reacting said carboxyalkylpyridine with an amine such as N,O-dimethylhydroxylamine hydrochloride in the presence of a suitable coupling agent system such as dicyclohexylcarbodiimide/hydroxybenztriazole to afford N-methoxy-N-methyl-pyridylalkylamide; reacting said N-methoxy-N-methyl-pyridylalkylamide with a Grignard reagent such as a phenylmagnesium bromide to afford a phenyl-pyridyl-alkanone; reacting said phenyl-pyridyl-alkanone with an isatin in the presence of potassium hydroxide in ethanol at elevated temperature of about 80-100° C. to afford a phenyl-pyridinyl-alkyl-quinoline-4-carboxylic acid; reacting said phenyl-pyridinyl-alkyl-quinoline-4-carboxylic acid with an appropriate amine in the presence of dicyclohexylcarbodiimide and hydroxybenztriazole, or other suitable dehydrating agent systems, to afford a compound of Formula I.
An exemplary process, to form a particular compound of Formula I is shown in Scheme 1:
Thus, as illustrated in Scheme 1, reaction of 3-pyridin-4-yl-propan-1-ol with an oxidizing agent such as CrO3 (Jones reagent) will afford 3-pyridin-4-yl-propionic acid, which can be reacted with N,O-dimethylhydroxylamine hydrochloride in the presence of a suitable coupling agent system such as dicyclohexylcarbodiimide/hydroxybenztriazole to afford N-methoxy-N-methyl-3-pyridin-4-yl-propionamide. This material can be reacted with a Grignard reagent such as phenylmagnesium bromide to afford 1-phenyl-3-pyridin-4-yl-propan-1-one. Reaction of this material with isatin in the presence of potassium hydroxide in ethanol at elevated temperature of about 80-100° C. will afford 2-phenyl-3-pyridin-4-yl-methyl-quinoline-4-carboxylic acid. The resulting material can be reacted with 1-phenyl-propylamine in the presence of dicyclohexylcarbodiimide and hydroxybenztriazole, or other suitable dehydrating agent systems, to afford 2-phenyl-3-pyridin-4-yl-methyl-quinoline-4-carboxylic acid (1-phenyl-propyl)-amide.
Generally, compounds of Formula I may be prepared by
coupling an acid of the structure
to an amine of the structure
by reaction in the presence of dicyclohexylcarbodiimide and hydroxybenztriazole to form a compound of Formula I.
In a further aspect the invention relates to compounds described herein wherein one or more of the atoms is a radioisotope of the same element. In a particular form of this aspect of the invention the compound is labeled with tritium. Such radio-labeled compounds are synthesized either by incorporating radio-labeled starting materials or, in the case of tritium, exchange of hydrogen for tritium by known methods. Known methods include (1) electrophilic halogenation, followed by reduction of the halogen in the presence of a tritium source, for example, by hydrogenation with tritium gas in the presence of a palladium catalyst, or (2) exchange of hydrogen for tritium performed in the presence of tritium gas and a suitable organometallic (e.g. palladium) catalyst.
Compounds of the invention labeled with tritium are useful for the discovery of novel medicinal compounds which bind to and modulate the activity, by agonism, partial agonism, or antagonism, of an NK-3 receptor. Such tritium-labeled compounds may be used in assays that measure the displacement of such compounds to assess the binding of ligands that bind to NK-3 receptors.
In a further aspect the invention relates to compounds described herein additionally comprising one or more atoms of a radioisotope. In a particular form of this aspect of the invention the compound comprises a radioactive halogen. Such radio-labeled compounds are synthesized by incorporating radio-labeled starting materials by known methods. Particular embodiments of this aspect of the invention are those in which the radioisotope is selected from 18F, 123I, 125I, 131I, 75Br, 76Br, 77Br or 82Br. A most particular embodiment of this aspect of the invention is that in which the radioisotope is 18F. Such compounds comprising one or more atoms of a radioisotope are useful as positron emission tomography (PET) ligands and for other uses and techniques to determine the location of NK3 receptors.
In another aspect the invention relates to compounds in accord with Formula I described herein and the use of such compounds in therapy and in compositions useful for therapy.
In another aspect the invention encompasses the use of compounds described herein for the therapy of diseases mediated through the action of NK-3 receptors. Such an aspect encompasses methods of treatment or prophylaxis of diseases or conditions in which modulation of the NK-3 receptor is beneficial which methods comprise administering a therapeutically-effective amount of an antagonistic compound of the invention to a subject suffering from said disease or condition.
One embodiment of this aspect of the invention is a method of treatment or prophylaxis of disorders, wherein the disorder is depression, anxiety, schizophrenia, cognitive disorders, psychoses, obesity, inflammatory diseases including irritable bowel syndrome and inflammatory bowel disorder, emesis, pre-eclampsia, chronic obstructive pulmonary disease, disorders associated with excessive gonadotrophins and/or androgens including dysmenorrhea, benign prostatic hyperplasia, prostatic cancer, or testicular cancer comprising administering a pharmacologically effective amount of a compound of Formula I to a patient in need thereof.
A further aspect of the invention is the use of a compound according to the invention, an enantiomer thereof or a pharmaceutically-acceptable salt thereof, for the treatment or prophylaxis of a disease or condition in which modulation of the NK-3 receptor is beneficial. Particular diseases and conditions that may be treated are depression, anxiety, schizophrenia, cognitive disorders, psychoses, obesity, inflammatory diseases including irritable bowel syndrome and inflammatory bowel disorder, emesis, pre-eclampsia, chronic obstructive pulmonary disease, disorders associated with excessive gonadotrophins and/or androgens including dysmenorrhea, benign prostatic hyperplasia, prostatic cancer, and testicular cancer. More particular embodiments encompass uses of a compound for treatment or prophylaxis of anxiety, depression, schizophrenia and obesity. A further aspect of the invention is the use of a compound according to the invention, an enantiomer thereof or a pharmaceutically-acceptable salt thereof, in the manufacture of a medicament for the treatment or prophylaxis of the diseases or conditions mentioned herein.
A particular embodiment of this aspect of the invention is the use of a compound of the invention in the manufacture of a medicament for treatment or prophylaxis of depression, anxiety, schizophrenia, cognitive disorders, psychoses, obesity, inflammatory diseases including irritable bowel syndrome and inflammatory bowel disorder, emesis, pre-eclampsia, chronic obstructive pulmonary disease, disorders associated with excessive gonadotrophins and/or androgens including dysmenorrhea, benign prostatic hyperplasia, prostatic cancer, and testicular cancer.
Compounds of the invention, enantiomers thereof, and pharmaceutically-acceptable salts thereof, may be used on their own or in the form of appropriate medicinal preparations for enteral or parenteral administration. According to a further aspect of the invention, there is provided a pharmaceutical composition including preferably less than 80% and more preferably less than 50% by weight of a compound of the invention in admixture with an inert pharmaceutically-acceptable diluent, lubricant or carrier.
Examples of diluents, lubricants and carriers are
There is also provided a process for the preparation of such a pharmaceutical composition which process comprises mixing or compounding the ingredients together and forming the mixed ingredients into tablets or suppositories, encapsulating the ingredients in capsules or dissolving the ingredients to form injectable solutions.
Pharmaceutically-acceptable derivatives include solvates and salts. For example, the compounds of the invention may form acid addition salts with acids, such as conventional pharmaceutically-acceptable acids including maleic, hydrochloric, hydrobromic, phosphoric, acetic, fumaric, salicylic, citric, lactic, mandelic, tartaric and methanesulfonic acids.
Acid addition salts of the compounds of Formula I which may be mentioned include salts of mineral acids, for example the hydrochloride and hydrobromide salts; and salts formed with organic acids such as formate, acetate, maleate, benzoate, tartrate, and fumarate salts. Acid addition salts of compounds of Formula I may be formed by reacting the free base or a salt, enantiomer or protected derivative thereof, with one or more equivalents of the appropriate acid. The reaction may be carried out in a solvent or medium in which the salt is insoluble or in a solvent in which the salt is soluble, e.g., water, dioxane, ethanol, tetrahydrofuran or diethyl ether, or a mixture of solvents, which may be removed in vacuum or by freeze drying. The reaction may be a metathetical process or it may be carried out on an ion exchange resin.
For the uses, methods, medicaments and compositions mentioned herein the amount of compound used and the dosage administered will, of course, vary with the compound employed, the mode of administration and the treatment desired. However, in general, satisfactory results are obtained when the compounds of the invention are administered at a daily dosage of about 0.1 mg to about 20 mg/kg of animal body weight. Such doses may be given in divided doses 1 to 4 times a day or in sustained release form. For man, the total daily dose is in the range of from 5 mg to 1,400 mg, more preferably from 10 mg to 100 mg, and unit dosage forms suitable for oral administration comprise from 2 mg to 1,400 mg of the compound admixed with a solid or liquid pharmaceutical carriers, lubricants and diluents.
Some compounds of the invention may exist in tautomeric, enantiomeric, stereoisomeric or geometric isomeric forms, all of which are included within the scope of the invention. The various optical isomers may be isolated by separation of a racemic mixture of the compounds using conventional techniques, e.g. fractional crystallization, or chiral HPLC. Alternatively the individual enantiomers may be made by reaction of the appropriate optically active starting materials under reaction conditions which will not cause racemization.
Exemplary compounds of the invention may be prepared by processes analogous to that described in Scheme 1. Those of skill in the art will readily appreciate that many suitable amines and acid chlorides and carboxylic acids may be used to form compounds within the scope of the subject matter described herein as Formula I.
The exemplary compounds and processes describe the invention by way of illustration and example for clarity of understanding. However to those skilled in the art, upon contemplation of the teaching of compounds, processes and methods of this invention, modifications and changes will be apparent that may be made thereto without departing from the spirit or scope of the invention.
The compound of Example 37 was prepared in accord with the following scheme:
To a mixture of 3-pyridin-4-ylpropionic acid (3.91 g, 25.8 mmol) and N-hydroxybenzotriazole (3.49 g, 25.8 mmol) in N,N-dimethylformamide (75 mL) was added N,N′-dicyclohexylcarbodiimide (5.33 g, 25.8 mmol). After agitating the mixture for 4 h, N,O-dimethylhydroxylamine hydrochloride (3.78 g, 38.8 mmol) and triethylamine (9 mL, 64.6 mmol) were added and the resulting slurry mixed overnight using a wrist shaker. The mixture was quenched by addition of water (10 mL) and concentrated under reduced pressure with heating at 70° C. to remove most of the N,N-dimethylformamide. The resulting slurry was partitioned between methylene chloride and water, which had been acidified with 1 M HCl. The aqueous layer was basified by addition of 1 M NaOH and extracted with methylene chloride. The organic layer was dried (MgSO4), filtered, and concentrated to an oil which was purified by flash silica chromatography using a gradient elution with 0.5% to 10% methanol in methylene chloride to afford the product as an oil (3.27 g, 17 mmol). 1H NMR (300 MHz, CDCl3) δ 8.50 (d, J=5.7 Hz, 2H), 7.16 (d, J=5.8 Hz, 2H), 3.64 (s, 3H), 3.18 (s, 3H), 2.97 (t, J=7.6 Hz, 2H), 2.76 (t, J=7.5 Hz, 3H).
To a cooled (−78° C.) solution of N-methoxy-N-methyl-3-pyridin-4-ylpropionamide in tetrahydrofuran (150 mL) was added a solution of phenyl magnesium bromide (3 M, 9.5 mL) over 5 min. The solution was warmed to 4° C. and stirred for 1 h. After this time, the solution was cooled to −78° C. and a second portion of phenyl magnesium bromide (3 M, 9.5 mL) was added. The solution was warmed to and stirred at 4° C. and stirred for an additional 1 h. To this was added saturated ammonium chloride (30 mL), warmed to room temperature, and the pH was adjusted to 8.8. The mixture was extracted with diethyl ether, dried (MgSO4), filtered, and concentrated to an orange oil which was purified by flash silica chromatography using a gradient elution of 0.5% to 5% methanol in methylene chloride to afford the product as a yellow foamy solid (1.58 g, 7.5 mmol). 1H NMR (300 MHz, CDCl3) δ 8.51 (d, J=4.7 Hz, 2H), 7.95 (d, J=8.6 Hz, 2H), 7.58 (t, J=7.4 Hz, 1H), 7.47 (t, J=6.7 Hz, 2H), 7.19 (d, J=5.8 Hz, 2H), 3.33 (t, J=7.5 Hz, 2H), 3.08 (t, J=7.4 Hz, 2H). LRMS m/z 212.1.
A mixture of 1-phenyl-3-pyridin-4-ylpropan-1-one (150 mg, 0.71 mmol), isatin (105 mg, 0.71 mmol) and KOH (120 mg, 2.1 mmol) in ethanol (3 mL) was heated in a sealed tube at 100° C. for 1.5 h. The mixture was concentrated under reduced pressure and diluted with water, then the pH was adjusted to 3.0. From the resulting mixture, the supernatant was purified by reverse phase HPLC with gradient elution of water and acetonitrile (containing 0.1% TFA) to afford the product as the trifluoroacetate salt as a yellow sticky powder (60 mg, 0.18 mmol). 1H NMR (300 MHz, DMSO) δ 8.58 (d, J=5.6 Hz, 2H), 8.11 (d, J=8.1 Hz, 1H), 7.97-7.86 (m, 2H), 7.77 (t, J=7.6 Hz, 1H), 7.35 (s, 7H), 4.44 (s, 2H). LRMS m/z 341.1.
To prepare the title compound (1) a solution of 2-phenyl-3-(pyridin-4-ylmethyl)quinoline-4-carboxylic acid trifluoroacetate salt was dissolved in 1 M HCl, concentrated under reduced pressure, re-dissolved in a mixture of water and acetonitrile and lyophilized to afford 2-phenyl-3-pyridin-4-ylmethyl-quinoline-4-carboxylic acid hydrochloride salt. A solution of this material (100 mg, 0.29 mmol) and thionyl chloride (26 μL, 0.35 mmol) in tetrahydrofuran (3 mL) was stirred for 1 h. After this time triethylamine (123 μL, 0.88 mmol) and (s)-1-phenyl-propylamine (79 mg, 0.59 mmol) were added and the mixture heated in a sealed tube at 70° C. for 3 h. The mixture was concentrated and purified by reverse phase HPLC with gradient elution of water and acetonitrile (containing 0.1% trifluoroacetic acid) to afford the product as the bis-trifluoroacetate salt. 1H NMR (300 MHz, DMSO, mixture of atropisomers; no peak integrations are reported) δ 9.42 (d, J=8.3 Hz), 9.17 (d, J=11.8 Hz), 8.89 (d, J=8.9 Hz), 8.47 (d, J=6.1 Hz), 8.39 (d, J=6.1 Hz), 8.23 (s), 8.11-7.99 (m), 7.99-7.76 (m), 7.50-6.89 (m), 5.05-4.96 (m), 4.36 (s), 1.82-1.68 (m), 1.86-1.65 (m), 1.27-1.15 (m), 1.01-0.95 (m), 0.81-0.73 (m). HRMS m/z 458.2201, calcd for C31H27N3O 458.2232.
Generally, NK-3r binding activity may be assessed using assays performed as described in Krause et al (Proc. Natl. Acad. Sci. USA 94: 310-315, 1997). NK-3r complementary DNA is cloned from human hypothalamic RNA using standard procedures. The receptor cDNA is inserted into a suitable expression vector transfected into a Chinese hamster ovary cell line, and a stably-expressing clonal cell line may be isolated, characterized and used for experiments.
Cells may be grown in tissue culture medium by techniques known to those of skill in the art and recovered by low speed centrifugation. Cell pellets may be homogenized, total cellular membranes isolated by high speed centrifugation and resuspended in buffered saline. Generally, receptor binding assays may be performed by incubating suitable amounts of purified membrane preparations with 125I-methylPhe7-neurokinin B, in the presence or absence of test compounds. Membrane proteins may be harvested by rapid filtration and radioactivity may be quantitated in a β-plate scintillation counter. Nonspecific binding may be distinguished from specific binding by use of suitable controls and the affinity of compounds for the expressed receptor may be determined by using different concentrations of compounds.
Preparation of Membranes from CHO Cells Transfected with Cloned NK-3 Receptors:
A human NK-3 receptor gene was cloned using methods similar to those described for other human NK receptors (Aharony et al., Mol. Pharmacol. 45:9-19, 1994; Caccese et al., Neuropeptides 33, 239-243, 1999). The DNA sequence of the cloned NK-3 receptor differed from the published sequence (Buell et al., FEBS Letts. 299, 90-95, 1992; Huang et al., Biochem. Biophys. Res. Commun. 184, 966-972, 1992) having a silent single T>C base change at nucleotide 1320 of the coding sequence. Since the change is silent, the cloned gene provides a primary amino acid sequence for the encoded NK-3 receptor protein identical to the published sequence. The receptor cDNA was used to transfect CHO-K1 cells using standard methods and a clone stably-expressing the receptor was isolated and characterized. Plasma membranes from these cells were prepared as published (Aharony et al., 1994).
Cells were harvested and centrifuged to remove medium. The pelleted cells were homogenized (Brinkman Polytron, three 15 sec bursts on ice) in a buffer consisting of 50 mM Tris-HCl (pH 7.4), 120 mM NaCl, 5 mM KCl, 10 mM EDTA and protease inhibitors (0.1 mg/ml soybean trypsin inhibitor, and 1 mM iodoacetamide). The homogenate was centrifuged at 1000×g for 10 min at 4° C. to remove cell debris. Pellets were washed once with homogenizing buffer. Supernatants were combined and centrifuged at 40,000×g for 20 min at 4° C. The membrane-containing pellet was homogenized with a Polytron as before. The suspension was centrifuged at 40,000×g for 20 min at 4° C. and resuspended in buffer (20 mM HEPES, pH 7.4 containing 3 mM MgCl2, 30 mM KCl, and 100 μM thiorphan) and the protein concentration determined. The membrane suspension was then diluted to 3 mg/ml with buffer containing 0.02% BSA, and flash frozen. Samples were stored at −80° C. until used.
A receptor binding assay method with [125I]-MePhe7-NKB was modified from that described by Aharony et al., J. Pharmacol. Exper. Ther., 274:1216-1221, 1995.
Competition experiments were carried out in 0.2 mL assay buffer (50 mM Tris-HCl, 4 mM MnCl2, 10 M thiorphan, pH 7.4) containing membranes (2 μg protein/reaction), tested competitors, and [125I]-MePhe7NKB (0.2 nM). Unlabeled homologue ligand (0.5 μM) was used to define nonspecific binding. Incubations were carried out at 25° C. for 90 min. Receptor-bound ligand was isolated by vacuum filtration in a Packard Harvester onto GF/C plates presoaked in 0.5% BSA. Plates were washed with 0.02 M Tris, pH 7.4. Computation of equilibrium binding constants (KD and Ki), receptor density (Bmax), and statistical analysis was carried out as published previously (Aharony et al., 1995) using GraphPad Prism or IDBS XLfit software.
Generally, NK-3 functional activity may be assessed by using calcium mobilization assays in stable NK-3r-expressing cell lines. Calcium mobilization induced by the methylPhe7-neurokinin B agonist may be monitored using a FLIPR (Molecular Devices) instrument in the manner described by the manufacturer. Agonists may be added to the cells and fluorescence responses continuously recorded for up to 5 min. The actions of antagonists may be assessed by preincubating cells prior to administration of the methylPhe7-neurokinin B agonist. The action of agonists may be assessed by observing their intrinsic activity in such a system.
NK-3 receptor expressing CHO cells were maintained in growth media (Ham's F12 medium, 10% FBS, 2 mM L-glutamine, and 50 mg/mL Hygromycin B). One day prior to the assay cells were dispensed into 384-well plates in Ultraculture media (Cambrex Bio Science) with 2 mM L-glutamine to achieve 70-90% confluency. To quantify NK-3 receptor-induced calcium mobilization, cells were first washed with assay buffer consisting of Hanks Balanced Salt Solution, 15 mM HEPES, and 2.5 mM probenecid, pH 7.4. The cells were then loaded with Fluo4/AM dye (4.4 μM) in assay buffer. Cells were incubated for one hour and then washed with assay buffer, exposed to 0.02-300 nM senktide and the fluorescence response recorded using a FLIPR instrument (Molecular Devices Corporation). To quantify antagonism of the agonist response, cells were preincubated with varying concentrations of test compound for 2-20 min and then exposed to 2 nM senktide, a concentration that alone elicits about an 70% maximal calcium response. The resulting data was analyzed using XLfit software (IDBS manufacturer) to determine EC50 and IC50 values.
| Filing Document | Filing Date | Country | Kind | 371c Date |
|---|---|---|---|---|
| PCT/SE06/00935 | 8/9/2006 | WO | 00 | 2/7/2008 |
| Number | Date | Country | |
|---|---|---|---|
| 60707383 | Aug 2005 | US |
