Patent Grant
11453900
The content of the following submission on ASCII text file is incorporated herein by reference in its entirety: a computer readable form (CRF) of the Sequence Listing (file name: 37847516001WOSEQLIST.txt, date recorded: Sep. 9, 2015, size: 185,381 bytes).
The invention provides compositions and methods for producing purified oligosaccharides, in particular fucosylated oligosaccharides that are typically found in human milk.
Human milk contains a diverse and abundant set of neutral and acidic oligosaccharides (Kunz, C., et al. (2000). Oligosaccharides in human milk: structural, functional, and metabolic aspects. Annu Rev Nutr 20, 699-722.; Bode, L., and Jantscher-Krenn, E. (2012). Structure-function relationships of human milk oligosaccharides. Adv Nutr 3, 383S-391S.). More than 130 different complex oligosaccharides have been identified in human milk, and their structural diversity and abundance is unique to humans. Although these molecules are likely not utilized by infants for nutrition, they nevertheless serve critical roles in the establishment of a healthy gut microbiome, in the prevention of disease, and in immune function (Gnoth, M. J., et al. (2000). Human milk oligosaccharides are minimally digested in vitro. J Nutr 130, 3014-020.; Newburg, D. S., and Walker, W. A. (2007). Protection of the neonate by the innate immune system of developing gut and of human milk. Pediatr Res 61, 2-8.; Bode, L., and Jantscher-Krenn, E. (2012). Structure-function relationships of human milk oligosaccharides. Adv Nutr 3, 383S-391S.; Rudloff, S., and Kunz, C. (2012). Milk oligosaccharides and metabolism in infants. Adv Nutr 3, 398S-405S.).
Human milk oligosaccharides (HMOS) include α(1,3) glycosylated oligosaccharides. For example, the human milk oligosaccharide (HMO) 3-fucosyllactose (3FL) is one of the most abundant fucosylated oligosaccharides present in human milk, and is thought to function with other HMOS to promote the growth of beneficial commensal bacteria in the infant gut. Additional α(1,3) fucosylated oligosaccharides include lactodifucotetraose (LDFT) and lacto-N-fucopentaose III (LNF III).
Prior to the invention described herein, the ability to produce human milk oligosaccharides (HMOS) inexpensively was problematic. For example, their production through chemical synthesis was limited by stereo-specificity issues, precursor availability, product impurities, and high overall cost. As an alternative to chemical synthesis, bacteria can be metabolically engineered to produce HMOS. A few glycosyltransferases derived from bacterial species have been identified and characterized in terms of their ability to catalyze the biosynthesis of HMOS in E. coli host strains. However, the high cost of reactants limits their utility for low-cost, large-scale production. Moreover, the previously available α(1,3) fucosyltransferases exhibit disadvantages including low yield and poor specificity for the location of α-fucose linkage formation. As a result, purity as well as yield of the desired α(1,3) fucosylated product is therefore compromised
As such, there exists a pressing need for new strategies to inexpensively manufacture large quantities of HMOS, in particular α(1,3) fucosylated oligosaccharides.
In a first aspect, the invention provides purified α(1,3) fucosyltransferase enzymes (also referred to herein as α(1,3) FTs) that utilize lactose and catalyze the transfer of an L-fucose sugar from a GDP-fucose donor substrate in an α1,3 linkage. Preferably, the acceptor substrate is an oligosaccharide. The α(1,3) fucosyltransferases identified and described herein are useful for expressing in host bacteria for the production of human milk oligosaccharides (HMOS). The α(1,3) fucosyltransferases are heterologous with respect to a host organism in which they are expressed produced. For example, the nucleic acid and/or amino acid sequences of the fucosyltransferases are different from those that naturally occur in the host bacteria. Thus, the host bacteria are genetically-altered; for example, they have been altered to include heterologous fucosyltransferase encoding DNA such as cDNA. Exemplary fucosylated oligosaccharides produced by the methods of the invention include 3-fucosyllactose (3FL), lactodifucotetraose (LDFT) and lacto-N-fucopentaose III (LNF III).
For example, the invention provides a composition for use in the production of a fucosylated oligosaccharide. The composition includes a bacterium expressing at least one α(1,3) fucosyltransferase enzyme, wherein the amino acid sequence of the one or more enzymes comprises at least 25% identity up to 100% identity to full length CafC (SEQ ID NO: 2), an isolated nucleic acid (e.g., a cDNA) encoding the enzyme or enzymes, or the purified recombinant enzyme itself or combination of enzymes. In some examples, the bacterium expresses two or more α(1,3) fucosyltransferase enzymes, wherein the amino acid sequence of one of the enzymes has at least 25% identity up to 100% identity to full length CafC (SEQ ID NO: 2), and the amino acid sequence of the one or more additional enzymes comprises at least 25% identity up to 100% identity to full length SEQ ID NOS: 2 (CafC), 17 (CafV), 9 (CafN), 7 (CafL), 10 (CafO), 12 (CafQ), 16 (CafU) or 53 (CafD). In the latter case, an advantage of increased (e.g., 10%, 25%, 50%, 75%, 2-fold, 3-fold or more greater) enzyme production or activity is observed with at least 2 copies of a α(1,3) fucosyltransferase enzyme-encoding sequences. For example, the α(1,3) fucosyltransferase enzyme-encoding sequences are different heterologous sequences. Furthermore, the two or more α(1,3) fucosyltransferase enzymes may be under control of the PL promoter and the bacterium may harbor the expression vector pG420.
The invention further provides methods for producing a fucosylated oligosaccharide in any of the bacteria disclosed herein, in such methods a bacterium may fermented in the presence of a nitrogen-rich nutritional additives comprising casamino acids or yeast extract. Additional examples of nitrogen-rich nutritional additives include protein hydrolysates of meat, casein, whey, gelatin, soybean, yeast or grain.
The α(1,3) fucosyltransferases of the invention comprise an amino acid sequence comprising at least 10% sequence identity and up to 100% sequence identity to CafC (SEQ ID NO: 2). Preferably, the α(1,3) fucosyltransferases of the invention comprise at least 50% sequence identity to CafC, more preferably less than 60%, 75%, 90%, 95%, and 99% sequence identity to CafC (SEQ ID NO:2). The α(1,3) fucosyltransferases of the invention retain the functional characteristic of catalyzing the formation of an α(1,3) linkage at the 3 position of glucose or GlcNAc. Preferably, the enzyme comprises the amino acid sequence of “FVDFWENFD” (SEQ ID NO: 57), “YHNCTKIFYSGENITPDFNICDYAIGFNFLSFGDRYIRIPFY” (SEQ ID NO:58), and “RKFCSFVVSNAKGAPERERFFQLLSEYKQVDSGGRYKNNVGGPVPDKTAFIKDYKF NIAFENSMCDGYTTEKIMEPMLVNSVPIYWG” (SEQ ID NO: 59), corresponding to the substrate binding and catalytic domains of CafC.
In a particularly preferred aspect, the α(1,3) fucosyltransferases of the invention comprise the amino acid sequence of SEQ ID NO: 2 (CafC), SEQ ID NO: 17 (CafV) and SEQ ID NO: 9 (CafN). Alternatively, the α(1,3) fucosyltransferases of the invention comprise SEQ ID NO: 7 (CafL), SEQ ID NO: 10 (CafO) and SEQ ID NO: 12 (CafQ).
In another particularly preferred aspect, the α(1,3) fucosyltransferase of the invention comprise the amino acid sequence of SEQ ID NO: 53 (CafD):
The amino acid sequence of the α(1,3) fucosyltransferase enzymes of the invention is at least 5%, at least 65, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70% at least 75%, at least 80%, at least 85%, at least 90%, at least 95% identical to the sequence of SEQ ID NO: 2, 9 or 17. Preferably the amino acid sequence is at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95% or 99% identical to the sequence of SEQ ID NO: 2 (CafC).
Alternatively, the α(1,3) fucosyltransferase comprises at least at least 15%, at least 20%, at least 25%, at least 30%, at least 355, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70% at least 75%, at least 80%, at least 85%, at least 90%, at least 95% sequence identity to any one of the novel α(1,3) fucosyltransferases disclosed herein, for example having the amino acid sequences listed in Table 1. The fucosylated oligosaccharides are preferably isolated and purified.
The α(1,3) fucosyltransferases of the invention include the amino acid sequences of the α(1,3) fucosyltransferases as well as fragments and variants thereof that exhibit α(1,3) fucosyltransferase activity.
In a second aspect, the invention provides a method for producing fucosylated oligosaccharides, in particular for producing α(1,3)-fucosylated oligosaccharides. The method comprises providing a bacterium that expresses at least one exogenous lactose-utilizing α(1,3) fucosyltransferase according to the invention and culturing the bacterium in the presence of lactose so as to produce one or more α(1,3)-fucosylated oligosaccharides. The method preferably further comprises retrieving or purifying the fucosylated oligosaccharide from said bacterium or from a culture supernatant of said bacterium.
In a related aspect, the invention provides methods for producing α(1,3)-fucosylated oligosaccharides utilizing a bacterial strain harboring an expression plasmid containing two different different α(1,3) fucosyltransferases in a “tandem” arrangement. These tandem (1,3) fucosyltransferases may be under the control of the PL promoter. An example expression vector comprising tandem (1,3) fucosyltransferases and a PL promoter is pG420. In a preferred embodiment, these tandem α(1,3) fucosyltransferases are CafC and CafN.
Furthermore, methods of the invention provide for eliminating added tryptophan in culture of strains producing high levels of α(1,3) fucosyltransferases and thereby repressing a PL promoter and minimizing cellular toxicity.
Optionally, the bacterium also expresses one or more exogenous lactose-utilizing α(1,2) fucosyltransferase enzymes and/or one or more exogenous lactose-utilizing α(1,4) fucosyltransferase enzymes. The combination of fucosyltransferases expressed in the production bacterium is dependent upon the desired fucosylated oligosaccharide product Examples of suitable α(1,2) fucosyltransferase enzymes include those described in U.S. Ser. No. 61/993,742, filed on May 15, 2014 (hereby incorporated by reference), but are not limited to Bacteroides vulgatus ATCC 8482 FutN (Genbank accession: YP_001300461.1), Parabacteroides johnsonii CL02T12C29 FutX (Genbank accession: WP 008155883.1), Lachnospiraceae bacterium 3_1_57FAA_CT1 FutQ (Genbank accession: WP 009251343.1), Prevotella melaninogenica ATCC 25845 FutO (Genbank accession: YP_003814512.1), Prevotella sp. CAG:891 FutW (Genbank accession: WP_022481266.1) and Bacteroides sp. CAG:63 FutZA (Genbank accession: WP_022161880.1). Examples of suitable α(1,4) fucosyltransferase enzymes include, but are not limited to H. pylori UA948 FucTa (which has a relaxed acceptor specificity and is able to generate both α(1,3)- and α(1,4)-fucosyl linkages). An example of an enzyme possessing only α(1,4) fucosyltransferase activity is given by the FucT III enzyme from Helicobacter pylori strain DMS6709 (e.g., GenBank Accession Number AY450598.1 (GI:40646733), incorporated herein by reference) (S. Rabbani, V. Miksa, B. Wipf, B. Ernst, Glycobiology 15, 1076-83 (2005). Alternatively, the α(1,3) fucosyltransferase also exhibits α(1,2) fucosyltransferase and/or α(1,4) fucosyltransferase activity.
In a third aspect, nucleic acid sequences encoding the α(1,3) fucosyltransferases are provided.
In a fourth aspect, the invention provides a nucleic acid construct, or vector, comprising an isolated nucleic acid encoding a lactose-accepting α(1,3) fucosyltransferase enzyme or variant, or fragment thereof, said nucleic acid being operably linked to one or more heterologous control sequences that direct production of the enzyme in a host bacteria production strain. The vector can further include one or more regulatory elements, e.g., a heterologous promoter. By “heterologous” is meant that the control sequence and protein-encoding sequence originate from different bacterial strains. The regulatory elements can be operably linked to a gene encoding a protein, a gene construct encoding a fusion protein gene, or a series of genes linked in an operon in order to express the fusion protein.
In a fifth aspect, the invention comprises an isolated recombinant cell, e.g., a bacterial cell containing an aforementioned nucleic acid molecule, construct or vector. The nucleic acid is optionally integrated into the genome of the host bacterium.
The fucosylated oligosaccharide produced by the engineered bacterium is preferably 3-fucosyllactose (3FL), lactodifucotetraose (LDFT) or lacto-N-fucopentaose III (LNF III). For example, for expression of 3FL, the bacterium is engineered to express an α(1,3) fucosyltransferase according to the invention. For example, to produce LDFT, the host bacterium is engineered to express an exogenous α(1,2) fucosyltransferase that also possesses α(1,3) fucosyltransferase activity, or an exogenous α(1,2) fucosyltransferase and an exogenous α(1,3) fucosyltransferase. For the production of LNF III, the host bacterium is preferably engineered to express an α(1,3) fucosyltransferase that is Helicobacter hepaticas ATCC 51449 CafD (SEQ ID NO: 53) (Genbank accession: AAP76669) or an α(1,3) fucosyltransferase which has 95%, 90%, 85%, 80%, 75%, 70%, 65%, 60%, 55% or 50% sequence identity with CafD and which retains the ability to catalyze the attachment of fucose to the GlcNAc moiety of lacto-N-neohexaose (LNnT).
Large quantities of 3-fucosyllactose (3FL), lactodifucotetraose (LDFT) or lacto-N-fucopentaose III (LNF III) are produced in bacterial hosts, e.g., an E. coli bacterium comprising an exogenous α(1,3) fucosyltransferase gene.
As described in detail below, E. coli (or other bacteria) is engineered to produce selected fucosylated oligosaccharides (including 3-fucosyllactose (3FL), lactodifucotetraose and lacto-N-fucopentaose III (LNF III)) in commercially viable levels. For example, yields are >5 grams/liter in a bacterial fermentation process. In other embodiments, the yields are greater than 10 grams/liter, greater than 15 grams/liter, greater than 20 grams/liter, greater than 25 grams/liter, greater than 30 grams/liter, greater than 35 grams/liter, greater than 40 grams/liter, greater than 45 grams/liter, greater than 50 grams/liter, greater than 55 grams/liter, greater than 60 grams/liter, greater than 65 grams/liter, greater than 70 grams/liter, or greater than 75 grams/liter of fucosylated oligosaccharide products, such as 3-fucosyllactose (3 FL), lactodifucotetraose and lacto-N-fucopentaose III (LNF III).
A suitable production host bacterial strain is one that is not the same bacterial strain as the source bacterial strain from which the fucosyltransferase-encoding nucleic acid sequence was identified. The host organism or cell used to express the lactose-accepting fucosyltransferase gene is typically the enterobacterium Escherichia coli K-12 (E. coli). E. coli K-12 is not considered a human or animal pathogen nor is it toxicogenic. E. coli K-12 is a standard production strain of bacteria and is noted for its safety due to its poor ability to colonize the colon and establish infections (see, e.g., epa.gov/oppt/biotech/pubs/fragra004.htm). However, a variety of bacterial species may be used in the oligosaccharide biosynthesis methods, e.g., Erwinia herbicola (Pantoea agglomerans), Citrobacter freundii, Pantoea citrea, Pectobacterium carotovorum, or Xanthomonas campestris. Bacteria of the genus Bacillus may also be used, including Bacillus subtilis, Bacillus licheniformis, Bacillus coagulans, Bacillus thermophilus, Bacillus laterosporus, Bacillus megaterium, Bacillus mycoides, Bacillus pumilus, Bacillus lentus, Bacillus cereus, and Bacillus circulans. Similarly, bacteria of the genera Lactobacillus and Lactococcus may be modified using the methods of this invention, including but not limited to Lactobacillus acidophilus, Lactobacillus salivarius, Lactobacillus plantarum, Lactobacillus helveticus, Lactobacillus delbrueckii, Lactobacillus rhamnosus, Lactobacillus bulgaricus, Lactobacillus crispatus, Lactobacillus gasseri, Lactobacillus casei, Lactobacillus reuteri, Lactobacillus jensenii, and Lactococcus lactis. Streptococcus thermophiles and Proprionibacterium freudenreichii are also suitable bacterial species for the invention described herein. Also included as part of this invention are strains, modified as described here, from the genera Enterococcus (e.g., Enterococcus faecium and Enterococcus thermophiles), Bifidobacterium (e.g., Bifidobacterium longum, Bifidobacterium infantis, and Bifidobacterium bifidum), Sporolactobacillus spp., Micromomospora spp., Micrococcus spp., Rhodococcus spp., and Pseudomonas (e.g., Pseudomonas fluorescens and Pseudomonas aeruginosa).
The bacterium utilized in the production methods described herein is preferably genetically engineered to increase the efficiency and yield of fucosylated oligosaccharide products. For example, the host production bacterium is characterized as having one, two, three or four of a reduced level of β-galactosidase activity, a defective colanic acid synthesis pathway, an inactivated ATP-dependent intracellular protease, an inactivated lacA. Preferably, the host production bacterium is characterized as having a reduced level of β-galactosidase activity, a defective colanic acid synthesis pathway, an inactivated ATP-dependent intracellular protease and an inactivated lacA.
A host bacterium suitable for the production systems described herein exhibits an enhanced or increased cytoplasmic or intracellular pool of lactose and/or GDP-fucose. For example, the bacterium is E. coli and endogenous E. coli metabolic pathways and genes are manipulated in ways that result in the generation of increased cytoplasmic concentrations of lactose and/or GDP-fucose, as compared to levels found in wild type E. coli. Preferably, the bacterium accumulates an increased intracellular lactose pool and an increased intracellular GDP-fucose pool. For example, the bacteria contain at least 10%, 20%, 50%, or 200%, 500%, 1000% or more of the levels of intracellular lactose and/or intracellular GDP-fucose compared to a corresponding wild type bacteria that lacks the genetic modifications described herein.
Increased intracellular concentration of lactose in the host bacterium compared to wild-type bacterium is achieved by manipulation of genes and pathways involved in lactose import, export and catabolism. In particular, described herein are methods of increasing intracellular lactose levels in E. coli genetically engineered to produce a human milk oligosaccharide by simultaneous deletion of the endogenous β-galactosidase gene (lacZ) and the lactose operon repressor gene (lacI). During construction of this deletion, the lacIq promoter is placed immediately upstream of (contiguous with) the lactose permease gene, lacY, i.e., the sequence of the lacIq promoter is directly upstream and adjacent to the start of the sequence encoding the lacY gene, such that the lacY gene is under transcriptional regulation by the lacIq promoter. The modified strain maintains its ability to transport lactose from the culture medium (via LacY), but is deleted for the wild-type chromosomal copy of the lacZ (encoding β-galactosidase) gene responsible for lactose catabolism. Thus, an intracellular lactose pool is created when the modified strain is cultured in the presence of exogenous lactose.
Another method for increasing the intracellular concentration of lactose in E. coli involves inactivation of the lacA gene. An inactivating mutation, null mutation, or deletion of lacA prevents the formation of intracellular acetyl-lactose, which not only removes this molecule as a contaminant from subsequent purifications, but also eliminates E. coli's ability to export excess lactose from its cytoplasm (Danchin A. Cells need safety valves. Bioessays 2009, July; 31(7):769-73.), thus greatly facilitating purposeful manipulations of the E. coli intracellular lactose pool.
In a further aspect, the invention also provides methods for increasing intracellular levels of GDP-fucose in a bacterium by manipulating the organism's endogenous colanic acid biosynthesis pathway. This increase is achieved through a number of genetic modifications of endogenous E. coli genes involved either directly in colanic acid precursor biosynthesis, or in overall control of the colanic acid synthetic regulon. Particularly preferred is inactivation of the genes or encoded polypeptides that act in the colanic acid synthesis pathway after the production of GDP-fucose (the donor substrate) and before the generation of colanic acid. Exemplary colanic acid synthesis genes include, but are not limited to: a wcaJ gene, (e.g., GenBank Accession Number (amino acid) BAA15900 (GI:1736749), incorporated herein by reference), a wcaA gene (e.g., GenBank Accession Number (amino acid) BAA15912.1 (GI:1736762), incorporated herein by reference), a wcaC gene (e.g., GenBank Accession Number (amino acid) BAE76574.1 (GI:85675203), incorporated herein by reference), a wcaE gene (e.g., GenBank Accession Number (amino acid) BAE76572.1 (GI:85675201), incorporated herein by reference), a wcaI gene (e.g., GenBank Accession Number (amino acid) BAA15906.1 (GI:1736756), incorporated herein by reference), a wcaL gene (e.g., GenBank Accession Number (amino acid) BAA15898.1 (GI:1736747), incorporated herein by reference), a wcaB gene (e.g., GenBank Accession Number (amino acid) BAA15911.1 (GI:1736761), incorporated herein by reference), a wcaF gene (e.g., GenBank Accession Number (amino acid) BAA15910.1 (GI:1736760), incorporated herein by reference), a wzxE gene (e.g., GenBank Accession Number (amino acid) BAE77506.1 (GI:85676256), incorporated herein by reference), a wzxC gene, (e.g., GenBank Accession Number (amino acid) BAA15899 (GI:1736748), incorporated herein by reference), a wcaD gene, (e.g., GenBank Accession Number (amino acid) BAE76573 (GI:85675202), incorporated herein by reference), a wza gene (e.g., GenBank Accession Number (amino acid) BAE76576 (GI:85675205), incorporated herein by reference), a wzb gene (e.g., GenBank Accession Number (amino acid) BAE76575 (GI:85675204), incorporated herein by reference), and a wzc gene (e.g., GenBank Accession Number (amino acid) BAA15913 (GI:1736763), incorporated herein by reference).
Preferably, the host bacterium, such as E. coli, comprises, or more preferably comprises in addition to the above-discussed genetic manipulations, inactivation of the wcaJ gene, which encoding the UDP-glucose lipid carrier transferase. The inactivation of the wcaJ gene can be by deletion of the gene, a null mutation, or inactivating mutation of the wcaJ gene, such that the activity of the encoded wcaJ is reduced or eliminated compared to wild-type E. coli. In a wcaJ null background, GDP-fucose accumulates in the E. coli cytoplasm.
Over-expression of a positive regulator protein, RcsA (e.g., GenBank Accession Number M58003 (GI:1103316), incorporated herein by reference), in the colanic acid synthesis pathway results in an increase in intracellular GDP-fucose levels. Over-expression of an additional positive regulator of colanic acid biosynthesis, namely RcsB (e.g., GenBank Accession Number E04821 (GI:2173017), incorporated herein by reference), is also utilized, either instead of or in addition to over-expression of RcsA, to increase intracellular GDP-fucose levels. Therefore, the host cell alternatively or additionally over-expresses RcsB and/or over-expresses RcsA.
Alternatively, colanic acid biosynthesis is increased following the introduction of a mutation into the E. coli lon gene (e.g., GenBank Accession Number L20572 (GI:304907), incorporated herein by reference). Lon is an adenosine-5′-triphosphate (ATP)-dependent intracellular protease that is responsible for degrading RcsA, mentioned above as a positive transcriptional regulator of colanic acid biosynthesis in E. coli. In a lon null background, RcsA is stabilized, RcsA levels increase, the genes responsible for GDP-fucose synthesis in E. coli are up-regulated, and intracellular GDP-fucose concentrations are enhanced. Mutations in lon suitable for use with the methods presented herein include null mutations or insertions that disrupt the expression or function of lon.
A functional lactose permease gene is preferably also present in the host bacterium. The lactose permease gene is an endogenous lactose permease gene or an exogenous lactose permease gene. For example, the lactose permease gene comprises an E. coli lacY gene (e.g., GenBank Accession Number V00295 (GI:41897), incorporated herein by reference). Many bacteria possess the inherent ability to transport lactose from the growth medium into the cell, by utilizing a transport protein that is either a homolog of the E. coli lactose permease (e.g., as found in Bacillus licheniformis), or a transporter that is a member of the ubiquitous PTS sugar transport family (e.g., as found in Lactobacillus casei and Lactobacillus rhamnosus). For bacteria lacking an inherent ability to transport extracellular lactose into the cell cytoplasm, this ability is conferred by an exogenous lactose transporter gene (e.g., E. coli lacY) provided on recombinant DNA constructs, and supplied either on a plasmid expression vector or as exogenous genes integrated into the host chromosome.
As described herein, the host bacterium preferably has a reduced level of β-galactosidase activity. When the bacterium is characterized by the deletion of the endogenous β-galactosidase gene, an exogenous β-galactosidase gene is introduced to the bacterium. For example, a plasmid expressing an exogenous β-galactosidase gene is introduced to the bacterium, or recombined or integrated into the host genome. For example, the exogenous β-galactosidase gene is inserted into a gene that is inactivated in the host bacterium, such as the lon gene.
The exogenous β-galactosidase gene is a functional β-galactosidase gene characterized by a reduced or low level of β-galactosidase activity compared to β-galactosidase activity in wild-type bacteria lacking any genetic manipulation. Exemplary β-galactosidase genes include E. coli lacZ and β-galactosidase genes from any of a number of other organisms (e.g., the lac4 gene of Kluyveromyces lactis (e.g., GenBank Accession Number M84410 (GI:173304), incorporated herein by reference) that catalyzes the hydrolysis of β-galactosides into monosaccharides. The level of β-galactosidase activity in wild-type E. coli bacteria is, for example, 1,000 units. Thus, the reduced β-galactosidase activity level encompassed by engineered host bacterium described herein includes less than 1,000 units, less than 900 units, less than 800 units, less than 700 units, less than 600 units, less than 500 units, less than 400 units, less than 300 units, less than 200 units, less than 100 units, or less than 50 units. Low, functional levels of β-galactosidase include β-galactosidase activity levels of between 0.05 and 1,000 units, e.g., between 0.05 and 750 units, between 0.05 and 500 units, between 0.05 and 400 units, between 0.05 and 300 units, between 0.05 and 200 units, between 0.05 and 100 units, between 0.05 and 50 units, between 0.05 and 10 units, between 0.05 and 5 units, between 0.05 and 4 units, between 0.05 and 3 units, or between 0.05 and 2 units of β-galactosidase activity. For unit definition and assays for determining β-galactosidase activity, see Miller J H, Laboratory CSH. Experiments in molecular genetics. Cold Spring Harbor Laboratory Cold Spring Harbor, N.Y.; 1972; (incorporated herein by reference). This low level of cytoplasmic β-galactosidase activity is not high enough to significantly diminish the intracellular lactose pool. The low level of β-galactosidase activity is very useful for the facile removal of undesired residual lactose at the end of fermentations. The art-recognized standard level of β-galactosidase activity in a wild-type bacterium is 1000 units. (See, Garcia et al., 2011, Biophysical J. 101:535-544). The art-recognized value for single copy wild type lac β-galactosidase activity is 1000 Miller units. By “low level” of β-galactosidase activity is meant less than 200 Miller units, i.e., less than 20% of wild type.
Optionally, the bacterium has, or additionally has, an inactivated thyA gene. Preferably, a mutation in a thyA gene in the host bacterium allows for the maintenance of plasmids that carry thyA as a selectable marker gene. Exemplary alternative selectable markers include antibiotic resistance genes such as BLA (beta-lactamase), or proBA genes (to complement a proAB host strain proline auxotropy) or purA (to complement a purA host strain adenine auxotrophy).
Most preferably, the host bacterium is an E. coli bacterium comprising the genotype ΔampC::PtrpBcI, Δ(lacI-lacZ)::FRT, PlacIqlacY+, ΔwcaJ::FRT, thyA::Tn10, Δlon:(npt3, lacZ+), and also expressing at least one of the exogenous α(1,3) fucosyltransferases described herein.
The bacterium comprising the above characteristics, most preferably the above characteristics in combination, is cultured in the presence of lactose. In some cases, the method further comprises culturing the bacterium in the presence of tryptophan and in the absence of thymidine.
In some cases, the culture medium is supplemented with a nitrogen-rich nutritional additive. High level expression (e.g. as driven from the induced PL promoter) of nearly all α(1,3) fucosyltransferases can be toxic to E. coli strains, resulting in poor viability and low 3-FL yields in fermentation runs. In some embodiments, supplementation of fermentation media with a nitrogen-rich additive such as casamino acids (CAA) or yeast extract (YE) protect against the toxic properties of α(1,3) fucosyltransferase activity, leading to significantly improved 3-FL production yields. In particular, CAA supplementation doubles the yield of 3FL obtained. In alternative embodiments, other such nitrogen-rich nutritional additives could include any protein hydrolysate (peptone) from a variety of sources, including but not limited to meat, casein, whey, gelatin, soybean, yeast and grains and/or extracts thereof. The fucosylated oligosaccharide is retrieved from the bacterium (i.e., a cell lysate) or from a culture supernatant of the bacterium. The fucosylated oligosaccharide is purified for use in therapeutic or nutritional products, or the bacteria are used directly in such products.
In another aspect, the invention provides a purified α(1,3) fucosylated oligosaccharide produced by the methods described herein. A “purified oligosaccharide”, e.g., 3-fucosyllactose (3FL), lactodifucotetraose (LDFT) or lacto-N-fucopentaose III (LNF III), is one that is at least 90%, 95%, 98%, 99%, or 100% (w/w) of the desired oligosaccharide by weight. Purity is assessed by any known method, e.g., thin layer chromatography or other chromatographic techniques known in the art. For example, an engineered bacterium, bacterial culture supernatant, or bacterial cell lysate according to the invention comprises 3-fucosyllactose (3FL), lactodifucotetraose (LDFT) or lacto-N-fucopentaose III (LNF III) produced by the methods described herein, and does not substantially comprise any other fucosylated oligosaccharides prior to purification of the fucosylated oligosaccharide products from the cell, culture supernatant, or lysate. As a general matter, the fucosylated oligosaccharide produced by the methods contains a negligible amount of 2′-FL in a 3-FL-containing cell, cell lysate or culture, or supernatant, e.g., less than 1% of the level of 3-FL or 0.5% of the level of 3-FL. Moreover, the fucosylated oligosaccharide produced by the methods described herein also have a minimal amount of contaminating lactose, which can often be co-purified with the fucosylated oligosaccharide product, such as 3-FL. This reduction in contaminating lactose results from the reduced level of β-galactosidase activity present in the engineered host bacterium. The fucosylated oligosaccharide is purified for use in therapeutic or nutritional products, or the bacterium is used directly in such products.
The invention includes a method of purifying a fucosylated oligosaccharide produced by the genetically engineered bacterium described above, which method comprises separating the desired fucosylated oligosaccharide (e.g., 3-FL) from contaminants in a bacterial cell lysate or bacterial cell culture supernatant of the bacterium.
The oligosaccharides are purified and used in a number of products for consumption by humans as well as animals, such as companion animals (dogs, cats) as well as livestock (bovine, equine, ovine, caprine, or porcine animals, as well as poultry). For example, a pharmaceutical composition comprises purified 3-fucosyllactose (3FL), lactodifucotetraose (LDFT) or lacto-N-fucopentaose III (LNF III) and a pharmaceutically-acceptable excipient that is suitable for oral administration.
In another aspect, the invention provides a method of producing a pharmaceutical composition comprising a purified human milk oligosaccharide (HMOS), said method comprising culturing the bacterium described above, purifying the HMOS produced by the bacterium, and combining the HMOS with an excipient or carrier to yield a dietary supplement for oral administration. These compositions are useful in methods of preventing or treating enteric and/or respiratory diseases in infants and adults. Accordingly, the compositions are administered to a subject suffering from or at risk of developing such a disease.
In yet another aspect, the invention also provides methods of identifying an α(1,3) fucosyltransferase gene capable of synthesizing fucosylated oligosaccharides in a host bacterium, e.g., 3-FL in E. coli. An exemplary method of identifying novel, lactose-utilizing α(1,3)fucosyltransferase enzyme comprises the following steps:
1) performing a computational search of sequence databases to define a broad group of simple sequence homologs of any single, known, lactose-utilizing α(1,3)fucosyltransferase;
2) using the list of search hits from step (1) to derive a search profile containing common sequence and/or structural motifs shared by the members of the list;
3) searching sequence databases, using the derived search profile based on the common sequence or structural motif from step (2) as query, and identifying additional candidate sequences, wherein a sequence homology to a reference lactose-utilizing α(1,3)fucosyltransferase is a predetermined percentage threshold;
4) compiling a list of candidate organisms of interest, said organisms being characterized as either expressing α(1,3)fucosyl-glycans in a naturally-occurring state, or whose natural habitat is known to include processes and interactions involving α(1,3)fucosyl-glycans;
5) selecting candidate sequences that are derived from candidate organisms of interest to generate a list of candidate lactose-utilizing enzymes;
6) expressing the candidate lactose-utilizing enzyme in a host organism; and
7) testing for lactose-utilizing α(1,3)fucosyltransferase activity, wherein detection of the desired fucosylated oligosaccharide product in said organism indicates that the candidate sequence comprises a novel lactose-utilizing α(1,3)fucosyltransferase. In another embodiment, the search profile is generated from a multiple sequence alignment of the amino acid sequences of more than one enzyme with known α(1,3)fucosyltransferase activity. The database search can then be designed to refine and iteratively search for novel α(1,3)fucosyltransferases with significant sequence similarity to the multiple sequence alignment query.
The predetermined percentage threshold in step (3) above is for example 50% or less, preferably less than 50%, more preferably 45% or less, more preferably 42% or less, or 40% or less. A particularly preferred percentage threshold is a sequence homology, or identity, of between 6 and 50%, more preferably between 6 and 42%.
In another aspect, the invention provides a method of treating, preventing, or reducing the risk of infection in a subject comprising administering to said subject a composition comprising a purified recombinant human milk oligosaccharide, wherein the HMOS binds to a pathogen and wherein the subject is infected with or at risk of infection with the pathogen. In one aspect, the infection is caused by a Norwalk-like virus or Campylobacter jejuni. The subject is preferably a mammal in need of such treatment. The mammal is, e.g., any mammal, e.g., a human, a primate, a mouse, a rat, a dog, a cat, a cow, a horse, or a pig. In a preferred embodiment, the mammal is a human. For example, the compositions are formulated into animal feed (e.g., pellets, kibble, mash) or animal food supplements for companion animals, e.g., dogs or cats, as well as livestock or animals grown for food consumption, e.g., cattle, sheep, pigs, chickens, and goats. Preferably, the purified HMOS is formulated into a powder (e.g., infant formula powder or adult nutritional supplement powder, each of which is mixed with a liquid such as water or juice prior to consumption) or in the form of tablets, capsules or pastes or is incorporated as a component in dairy products such as milk, cream, cheese, yogurt or kefir, or as a component in any beverage, or combined in a preparation containing live microbial cultures intended to serve as probiotics, or in prebiotic preparations to enhance the growth of beneficial microorganisms either in vitro or in vivo.
Polynucleotides, polypeptides, and oligosaccharides of the invention are purified and/or isolated. Purified defines a degree of sterility that is safe for administration to a human subject, e.g., lacking infectious or toxic agents. Specifically, as used herein, an “isolated” or “purified” nucleic acid molecule, polynucleotide, polypeptide, protein or oligosaccharide, is substantially free of other cellular material, or culture medium when produced by recombinant techniques, or chemical precursors or other chemicals when chemically synthesized. For example, purified HMOS compositions are at least 60% by weight (dry weight) the compound of interest. Preferably, the preparation is at least 75%, more preferably at least 90%, and most preferably at least 99%, by weight the compound of interest. Purity is measured by any appropriate standard method, for example, by column chromatography, thin layer chromatography, or high-performance liquid chromatography (HPLC) analysis. For example, a “purified protein” refers to a protein that has been separated from other proteins, lipids, and nucleic acids with which it is naturally associated. Preferably, the protein constitutes at least 10, 20, 50, 70, 80, 90, 95, 99-100% by dry weight of the purified preparation.
Similarly, by “substantially pure” is meant an oligosaccharide that has been separated from the components that naturally accompany it. Typically, the oligosaccharide is substantially pure when it is at least 60%, 70%, 80%, 90%, 95%, or even 99%, by weight, free from the proteins and naturally-occurring organic molecules with which it is naturally associated.
By “isolated nucleic acid” is meant a nucleic acid that is free of the genes which, in the naturally-occurring genome of the organism from which the DNA of the invention is derived, flank the gene. The term covers, for example: (a) a DNA which is part of a naturally occurring genomic DNA molecule, but is not flanked by both of the nucleic acid sequences that flank that part of the molecule in the genome of the organism in which it naturally occurs; (b) a nucleic acid incorporated into a vector or into the genomic DNA of a prokaryote or eukaryote in a manner, such that the resulting molecule is not identical to any naturally occurring vector or genomic DNA; (c) a separate molecule such as a cDNA, a genomic fragment, a fragment produced by polymerase chain reaction (PCR), or a restriction fragment; and (d) a recombinant nucleotide sequence that is part of a hybrid gene, i.e., a gene encoding a fusion protein. Isolated nucleic acid molecules according to the present invention further include molecules produced synthetically, as well as any nucleic acids that have been altered chemically and/or that have modified backbones.
A “heterologous promoter” is a promoter which is different from the promoter to which a gene or nucleic acid sequence is operably linked in nature.
The term “overexpress” or “overexpression” refers to a situation in which more factor is expressed by a genetically-altered cell than would be, under the same conditions, by a wild type cell. Similarly, if an unaltered cell does not express a factor that it is genetically altered to produce, the term “express” (as distinguished from “overexpress”) is used indicating the wild type cell did not express the factor at all prior to genetic manipulation.
As used herein, an “inactivated” or “inactivation of a” gene, encoded gene product (i.e., polypeptide), or pathway refers to reducing or eliminating the expression (i.e., transcription or translation), protein level (i.e., translation, rate of degradation), or enzymatic activity of the gene, gene product, or pathway. In the instance where a pathway is inactivated, preferably one enzyme or polypeptide in the pathway exhibits reduced or negligible activity. For example, the enzyme in the pathway is altered, deleted or mutated such that the product of the pathway is produced at low levels compared to a wild-type bacterium or an intact pathway. Alternatively, the product of the pathway is not produced. Inactivation of a gene is achieved by deletion or mutation of the gene or regulatory elements of the gene such that the gene is no longer transcribed or translated. Inactivation of a polypeptide can be achieved by deletion or mutation of the gene that encodes the gene product or mutation of the polypeptide to disrupt its activity. Inactivating mutations include additions, deletions or substitutions of one or more nucleotides or amino acids of a nucleic acid or amino acid sequence that results in the reduction or elimination of the expression or activity of the gene or polypeptide. In other embodiments, inactivation of a polypeptide is achieved through the addition of exogenous sequences (i.e., tags) to the N or C-terminus of the polypeptide such that the activity of the polypeptide is reduced or eliminated (i.e., by steric hindrance).
The terms “treating” and “treatment” as used herein refer to the administration of an agent or formulation to a clinically symptomatic individual afflicted with an adverse condition, disorder, or disease, so as to effect a reduction in severity and/or frequency of symptoms, eliminate the symptoms and/or their underlying cause, and/or facilitate improvement or remediation of damage. The terms “preventing” and “prevention” refer to the administration of an agent or composition to a clinically asymptomatic individual who is susceptible to a particular adverse condition, disorder, or disease, and thus relates to the prevention of the occurrence of symptoms and/or their underlying cause.
By the terms “effective amount” and “therapeutically effective amount” of a formulation or formulation component is meant a nontoxic but sufficient amount of the formulation or component to provide the desired effect.
The transitional term “comprising,” which is synonymous with “including,” “containing,” or “characterized by,” is inclusive or open-ended and does not exclude additional, unrecited elements or method steps. By contrast, the transitional phrase “consisting of” excludes any element, step, or ingredient not specified in the claim. The transitional phrase “consisting essentially of” limits the scope of a claim to the specified materials or steps “and those that do not materially affect the basic and novel characteristic(s)” of the claimed invention.
Other features and advantages of the invention will be apparent from the following description of the preferred embodiments thereof, and from the claims. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention, suitable methods and materials are described below.
All published foreign patents and patent applications cited herein are incorporated herein by reference. Genbank and NCBI submissions indicated by accession number cited herein are incorporated herein by reference. All other published references, documents, manuscripts and scientific literature cited herein are incorporated herein by reference. In the case of conflict, the present specification, including definitions, will control. In addition, the materials, methods, and examples are illustrative only and not intended to be limiting.
While some studies suggest that human milk glycans could be used as antimicrobial anti-adhesion agents, the difficulty and expense of producing adequate quantities of these agents of a quality suitable for human consumption has limited their full-scale testing and perceived utility. What has been needed is a suitable method for producing the appropriate glycans in sufficient quantities at reasonable cost. Prior to the invention described herein, there were attempts to use several distinct synthetic approaches for glycan synthesis. Some chemical approaches can synthesize oligosaccharides (Flowers, H. M. Methods Enzymol 50, 93-121 (1978); Seeberger, P. H. Chem Commun (Camb) 1115-1121 (2003)), but reactants for these methods are expensive and potentially toxic (Koeller, K. M. & Wong, C. H. Chem Rev 100, 4465-4494 (2000)).
Enzymes expressed from engineered organisms (Albermann, C., Piepersberg, W. & Wehmeier, U. F. Carbohydr Res 334, 97-103 (2001); Bettler, E., Samain, E., Chazalet, V., Bosso, C., et al. Glycoconj J 16, 205-212 (1999); Johnson, K. F. Glycoconj J 16, 141-146 (1999); Palcic, M. M. Curr Opin Biotechnol 10, 616-624 (1999); Wymer, N. & Toone, E. J. Curr Opin Chem Biol 4, 110-119 (2000)) provide a precise and efficient synthesis (Palcic, M. M. Curr Opin Biotechnol 10, 616-624 (1999)); Crout, D. H. & Vic, G. Curr Opin Chem Biol 2, 98-111 (1998)), but the high cost of the reactants, especially the sugar nucleotides, limits their utility for low-cost, large-scale production. Microbes have been genetically engineered to express the glycosyltransferases needed to synthesize oligosaccharides from the bacteria's innate pool of nucleotide sugars (Endo, T., Koizumi, S., Tabata, K., Kakita, S. & Ozaki, A. Carbohydr Res 330, 439-443 (2001); Endo, T., Koizumi, S., Tabata, K. & Ozaki, A. Appl Microbiol Biotechnol 53, 257-261 (2000); Endo, T. & Koizumi, S. Curr Opin Struct Biol 10, 536-541 (2000); Endo, T., Koizumi, S., Tabata, K., Kakita, S. & Ozaki, A. Carbohydr Res 316, 179-183 (1999); Koizumi, S., Endo, T., Tabata, K. & Ozaki, A. Nat Biotechnol 16, 847-850 (1998)).
One strategy for efficient, industrial-scale synthesis of HMOS is the metabolic engineering of bacteria. This approach involves the construction of microbial strains overexpressing heterologous glycosyltransferases, membrane transporters for the import of precursor sugars into the bacterial cytosol, and possessing enhanced pools of regenerating nucleotide sugars for use as biosynthetic precursors (Dumon, C., Samain, E., and Priem, B. (2004). Biotechnol Prog 20, 412-19; Ruffing, A., and Chen, R. R. (2006). Microb Cell Fact 5, 25). A key aspect of this approach is the heterologous glycosyltransferase selected for overexpression in the microbial host. The choice of glycosyltransferase can significantly affect the final yield of the desired synthesized oligosaccharide, given that enzymes can vary greatly in terms of kinetics, substrate specificity, affinity for donor and acceptor molecules, stability and solubility. A few glycosyltransferases derived from different bacterial species have been identified and characterized in terms of their ability to catalyze the biosynthesis of HMOS in E. coli host strains (Dumon, C., et al. (2006). Chembiochem 7, 359-365; Dumon, C., Samain, E., and Priem, B. (2004). Biotechnol Prog 20, 412-19; Li, M., Liu, X. W., Shao, J., Shen, J., Jia, Q., Yi, W., Song, J. K., Woodward, R., Chow, C. S., and Wang, P. G. (2008). Biochemistry 47, 378-387). The identification of additional glycosyltransferases with faster kinetics, greater affinity for nucleotide sugar donors and/or acceptor molecules, or greater stability within the bacterial host significantly improves the yields of therapeutically useful HMOS. Prior to the invention described herein, chemical syntheses of HMOS were possible, but were limited by stereo-specificity issues, precursor availability, product impurities, and high overall cost (Flowers, H. M. Methods Enzymol 50, 93-121 (1978); Seeberger, P. H. Chem Commun (Camb) 1115-1121 (2003); Koeller, K. M. & Wong, C. H. Chem Rev 100, 4465-4494 (2000)). The invention overcomes the shortcomings of these previous attempts by providing new strategies to inexpensively manufacture large quantities of human milk oligosaccharides (HMOS) for use as dietary supplements.
Prior to the invention described herein, there was a growing need to identify and characterize additional glycosyltransferases that are useful for the synthesis of HMOS in metabolically engineered bacterial hosts.
Advantages provided by the invention include efficient expression of the enzyme, improved stability and/or solubility of the fucosylated oligosaccharide product β-FL, LDFT and LNF III,) and reduced toxicity to the host organism. The invention features novel α(1,3) FTs suitable for expression in production strains for increased efficacy and yield of fucosylated HMOS compared to α(1,3) FTs currently utilized in the field.
Human Milk Glycans
Human milk contains a diverse and abundant set of neutral and acidic oligosaccharides (Kunz, C., Rudloff, S., Baier, W., Klein, N., and Strobel, S. (2000). Annu Rev Nutr 20, 699-722; Bode, L. (2006). J Nutr 136, 2127-130). More than 130 different complex oligosaccharides have been identified in human milk, and their structural diversity and abundance is unique to humans. Although these molecules may not be utilized directly by infants for nutrition, they nevertheless serve critical roles in the establishment of a healthy gut microbiome (Marcobal, A., Barboza, M., Froehlich, J. W., Block, D. E., et al. J Agric Food Chem 58, 5334-5340 (2010)), in the prevention of disease (Newburg, D. S., Ruiz-Palacios, G. M. & Morrow, A. L. Annu Rev Nutr 25, 37-58 (2005)), and in immune function (Newburg, D. S. & Walker, W. A. Pediatr Res 61, 2-8 (2007)). Despite millions of years of exposure to human milk oligosaccharides (HMOS), pathogens have yet to develop ways to circumvent the ability of HMOS to prevent adhesion to target cells and to inhibit infection. The ability to utilize HMOS as pathogen adherence inhibitors promises to address the current crisis of burgeoning antibiotic resistance. Human milk oligosaccharides produced by biosynthesis represent the lead compounds of a novel class of therapeutics against some of the most intractable scourges of society.
Role of Human Milk Glycans in Infectious Disease
Human milk glycans, which comprise both unbound oligosaccharides and their glycoconjugates, play a significant role in the protection and development of the infant gastrointestinal (GI) tract. Neutral fucosylated oligosaccharides, including α(1,3) fucosylated oligosaccharides, protect infants against several important pathogens. Milk oligosaccharides found in various mammals differ greatly, and the composition in humans is unique (Hamosh M., 2001 Pediatr Clin North Am, 48:69-86; Newburg D. S., 2001 Adv Exp Med Biol, 501:3-10). Moreover, glycan levels in human milk change throughout lactation and also vary widely among individuals (Morrow A. L. et al., 2004 J Pediatr, 145:297-303; Chaturvedi P et al., 2001 Glycobiology, 11:365-372). Approximately 200 distinct human milk oligosaccharides have been identified and combinations of simple epitopes are responsible for this diversity (Newburg D. S., 1999 Curr Med Chem, 6:117-127; Ninonuevo M. et al., 2006 J Agric Food Chem, 54:7471-74801).
Human milk oligosaccharides are composed of 5 monosaccharides: D-glucose (Glc), D-galactose (Gal), N-acetylglucosamine (GlcNAc), L-fucose (Fuc), and sialic acid (N-acetyl neuraminic acid, Neu5Ac, NANA). Human milk oligosaccharides are usually divided into two groups according to their chemical structures: neutral compounds containing Glc, Gal, GlcNAc, and Fuc, linked to a lactose (Galβ1-4Glc) core, and acidic compounds including the same sugars, and often the same core structures, plus NANA (Charlwood J. et al., 1999 Anal Biochem, 273:261-277; Martin-Sosa et al., 2003 J Dairy Sci, 86:52-59; Parkkinen J. and Finne J., 1987 Methods Enzymol, 138:289-300; Shen Z. et al., 2001 J Chromatogr A, 921:315-321).
Approximately 70-80% of oligosaccharides in human milk are fucosylated, and their synthetic pathways are believed to proceed as shown in
Human Milk Glycans Inhibit Binding of Enteropathogens to their Receptors
Human milk glycans have structural homology to cell receptors for enteropathogens and function as receptor decoys.
For example, 3-fucosyllactose (3FL) is one of the most abundant fucosylated oligosaccharides present in human milk and is thought to function with other HMOS to promote the growth of beneficial commensal bacteria in the infant gut, such as Bifidobacterium spp (Marcobal, A., et al. (2010). Consumption of human milk oligosaccharides by gut-related microbes. J Agric Food Chem 58, 5334-340.; Asakuma, S., et al. (2011). Physiology of the consumption of human milk oligosaccharides by infant-gut associated bifidobacteria. J Biol Chem; Sela, D. A., et al. (2012). Bifidobacterium longum subsp. infantis ATCC 15697 α-fucosidases are active on fucosylated human milk oligosaccharides. Appl Environ Microbiol 78, 795-803.; Garrido, D., et al. (2012). A molecular basis for bifidobacterial enrichment in the infant gastrointestinal tract. Adv Nutr 3, 415S-421S.). Indeed, it has been shown that 3FL can be utilized for growth by several different Bifidobacterium spp. In vitro when provided as the sole sugar source (Yu, Z. T., et al. (2012). The Principal Fucosylated Oligosaccharides of Human Milk Exhibit Prebiotic Properties on Cultured Infant Microbiota. Glycobiology). Furthermore, it has been demonstrated that 3FL was consumed in the context of an in vitro infant fecal microbiota culture system, providing further evidence that 3FL is a substrate for beneficial commensal microbes in the infant gut (Yu, Z. T., et al. (2012). The Principal Fucosylated Oligosaccharides of Human Milk Exhibit Prebiotic Properties on Cultured Infant Microbiota. Glycobiology). In addition, several bacterial and viral pathogens target host cell molecules with structural similarity to 3FL for cell-surface binding in the process of initiating infection. Several studies have shown that 3FL can prevent the binding of pathogens to their target molecules or host cells via a competition mechanism, suggesting that 3FL will also be useful as an anti-infective molecule (Huang et al, 2003; Coppa et al 2006; Chessa et al, 2008). Structurally, 3FL consists of a fucose molecule α1,3 linked to the glucose portion of lactose (Galβ1-4(Fucα1-3)Glc) (
LDFT is a di-fucosylated HMOS and has the structure Fucα1,2Galβ1,4(Fucα1,3)Glc. LDFT is one of the most abundant HMOS found in human milk (Newburg et al., 2000; Warren et al., 2001). LDFT has been shown to be utilized as a sugar source for growth in vitro by beneficial, commensal bacteria of the infant gut (i.e. Bifidobacteria spp.) and will therefore have utility as an important prebiotic, or “Bifidogenic” factor (Asakuma, S., et al. (2011). Physiology of the consumption of human milk oligosaccharides by infant-gut associated bifidobacteria. J Biol Chem; Yu, Z. T., et al. (2012). The Principal Fucosylated Oligosaccharides of Human Milk Exhibit Prebiotic Properties on Cultured Infant Microbiota. Glycobiology; Blank, D., et al. (2012). Human milk oligosaccharides and Lewis blood group: individual high-throughput sample profiling to enhance conclusions from functional studies. Adv Nutr 3, 440S-49S.). Furthermore, LDFT is structurally highly similar to the histo-blood group antigen Lewis Y (Ley). Many bacterial and viral pathogens target molecules on the surface of host cells with structural similarity to the Lewis Y epitope for binding in the process of initiating infection, such as at the lining of the gut. Orally administered LDFT could serve as a structural mimic of host cell receptors and therefore prevent the binding of pathogens to the intestinal epithelium via a competition mechanism (Ruiz-Palacios, G. M., et al. (2003). Campylobacter jejuni binds intestinal H(O) antigen (Fuc alpha 1, 2Gal beta 1, 4GlcNAc), and fucosyloligosaccharides of human milk inhibit its binding and infection. J Biol Chem 278, 14112-120.; Morrow, A. L., et al. (2004). Human milk oligosaccharide blood group epitopes and innate immune protection against campylobacter and calicivirus diarrhea in breastfed infants. Adv Exp Med Biol 554, 443-46.; Sharon, N. (2006). Carbohydrates as future anti-adhesion drugs for infectious diseases. Biochim Biophys Acta 1760, 527-537.; Bode, L., and Jantscher-Krenn, E. (2012). Structure-function relationships of human milk oligosaccharides. Adv Nutr 3, 383S-391S.).
LNF III has the structure Galβ1-4(Fucα1,3)GlcNacβ1-3Galβ1-4Glc, and contains the Lex antigen structure. LNF III is likely to serve as a prebiotic factor for the growth of commensal microbes in the infant gut, and also may prevent the binding of microbial pathogens to the intestinal epithelia via receptor mimicry.
Several pathogens utilize sialylated glycans as their host receptors, such as influenza (Couceiro, J. N., Paulson, J. C. & Baum, L. G. Virus Res 29, 155-165 (1993)), parainfluenza (Amonsen, M., Smith, D. F., Cummings, R. D. & Air, G. M. J Virol 81, 8341-8345 (2007), and rotoviruses (Kuhlenschmidt, T. B., Hanafin, W. P., Gelberg, H. B. & Kuhlenschmidt, M. S. Adv Exp Med Biol 473, 309-317 (1999)). The sialyl-Lewis X epitope is used by Helicobacter pylori (Mandavi, J., Sondén, B., Hurtig, M., Olfat, F. O., et al. Science 297, 573-578 (2002)), Pseudomonas aeruginosa (Scharfman, A., Delmotte, P., Beau, J., Lamblin, G., et al. Glycoconj J 17, 735-740 (2000)), and some strains of noroviruses (Rydell, G. E., Nilsson, J., Rodriguez-Diaz, J., Ruvoën-Clouet, N., et al. Glycobiology 19, 309-320 (2009)).
Identification of Novel α(1,3) Fucosyltransferases
The present invention provides novel α(1,3) fucosyltransferase enzymes (α(1,3) FTs). The α(1,3) FTs of the invention provide advantages over known α(1,3) fucosyltransferase enzymes, such advantages including improved yield, improved specificity, and reduced toxicity to host cells.
Not all α(1,3)fucosyltransferases can utilize lactose as an acceptor substrate. An acceptor substrate includes, for example, a carbohydrate, an oligosaccharide, a protein or glycoprotein, a lipid or glycolipid, e.g., N-acetylglucosamine, N-acetyllactosamine, galactose, fucose, sialic acid, glucose, lactose, or any combination thereof. A preferred alpha (1,3) fucosyltransferase utilizes GDP-fucose as a donor, and lactose is the acceptor for that donor.
A method of identifying novel α(1,2)fucosyltransferase enzymes capable of utilizing lactose as an acceptor was previously carried out (as described in PCT/US2013/051777, hereby incorporated by reference in its entirety) using the following steps: 1) performing a computational search of sequence databases to define a broad group of simple sequence homologs of any known, lactose-utilizing α(1,2)fucosyltransferase (e.g. in this case Helicobacter pylori 26695 FutC); 2) using the list of homologs from step 1 to derive a search profile containing common sequence and/or structural motifs shared by the members of the broad group, e.g. by using computer programs such as MEME (Multiple Em for Motif Elicitation. http://meme.sdsc.edu/meme/cgi-bin/meme.cgi (accessed Aug. 5, 2014)) or PSI-BLAST (Position-Specific Iterated BLAST) (Blast. http://ncbi.nlm.nih.gov/blast (accessed Aug. 4, 2014); with additional information at openstax CNX. http://cnx.org/content/m11040/latest/(accessed Aug. 5, 2014)); 3) searching sequence databases (e.g., using computer programs such as PSI-BLAST, or MAST (Motif Alignment Search Tool. http://meme.sdsc.edu/meme/cgi-bin/mast.cgi (accessed Aug. 5, 2014)); using this derived search profile as query, and identifying “candidate sequences” whose simple sequence homology to the original lactose-accepting α(1,2)fucosyltransferase is 50% or less; 4) scanning the scientific literature and developing a list of “candidate organisms” known to express α(1,2)fucosyl-glycans, or whose natural habitat is known to include processes and interactions involving α(1,2)fucosyl-glycans; 5) selecting only those “candidate sequences” that are derived from “candidate organisms” to generate a list of “candidate lactose-utilizing enzymes”; and 6) expressing each “candidate lactose-utilizing enzyme” and testing for lactose-utilizing α(1,2)fucosyltransferase activity.
The percentage sequence identity threshold in step (3) above is 50% or less, such as less than 50%. Preferably, the % sequence identity threshold is 45% or less, more preferably 42% or less. A preferred % sequence identity threshold is 6%-42%. The threshold was set to select candidate sequences which are more distantly-related to the query α(1,2)fucosyltransferase (e.g. in this case Helicobacter pylori 26695 FutC), and to exclude more closely related candidate sequences.
Example α(1,2) fucosyltransferases include but are not limited to: Helicobacter pylori FutC (GenBank Accession AAD29869.1; Helicobacter mustelae 12198 FutL (GenBank Accession YP_003517185.1); Bacteroides vulgatus ATCC 8482 FutN (GenBank Accession YP_001300461.1); Escherichia coli UMEA 3065-1 WbgL (GenBank Accession WP_021554465.1); Escherichia coli WbsJ (GenBank Accession AA037698.1); Prevotella melaninogenica ATCC 25845 FutO (GenBank Accession YP_003814512.1); Clostridium bolteae 90A9 FutP (GenBank Accession WP_002570768.1); Lachnospiraceae bacterium 3_1_57FAA_CT1 FutQ (GenBank Accession WP_009251343.1); Methanosphaerula palustris E1-9c FutR (GenBank Accession YP_002467213.1); Tannerella sp. CAG:118 FutS (GenbBank WP_021929367.1); Bacteroides caccae ATCC 43185 FutU (GenBank Accession WP_005675707.1); Butyrivibrio sp. AE2015 FutV (GenBank Accesion WP_022772718.1); Prevotella sp. CAG:891 FutW (GenBank Accession WP_022481266.1); Parabacteroides johnsonii CL02T12C29 FutX (GenBank Accession WP_008155883.1); Salmonella enterica subsp. enterica serovar Poona str. ATCC BAA-1673 FutZ (GenBank Accession WP_023214330.1); and Bacteroides sp. CAG:633 (GenBank Accesion WP_022161880.1).
The MEME suite of sequence analysis tools (MEME. http://meme.sdsc.edu/meme/cgi-bin/meme.cgi (accessed Aug. 5, 2014)) is optionally used as an alternative to PSI-BLAST. Sequence motifs are discovered using the program “MEME”. These motifs can then be used to search sequence databases using the program “MAST”. The BLAST and PSI-BLAST search algorithms are other well-known alternatives.
An α(1,3) FT from H. pylori strain 26695 termed FutA has been utilized by others to catalyze the synthesis of 3FL in metabolically engineered E. coli (Dumon, C. et al. (2006). Production of Lewis x tetrasaccharides by metabolically engineered Escherichia coli. Chembiochem 7, 359-365.; Dumon, C. et al. (2004). Assessment of the two Helicobacter pylori alpha-1,3-fucosyltransferase ortholog genes for the large-scale synthesis of LewisX human milk oligosaccharides by metabolically engineered Escherichia coli. Biotechnol Prog 20, 412-19.), however the overall yield of 3FL obtained using this enzyme is low. Moreover FutA is promiscuous in its specificity, i.e. the enzyme will not only form an α-fucose linkage at the 3-position of glucose at the reducing end of sugar acceptors, but additionally will form α-fucose linkages at the 3-position of internal N-acetyl-glucosamine (GlcNAc) moieties. Thus FutA cannot be utilized effectively for the production of lacto-N-fucopentaose III (LNF-III, Lewis X) using lacto-N-neotetraose (LNnT) as the acceptor sugar. In addition FutA also catalyzes, at a low level, the promiscuous insertion of an α-fucose linkage at the 2-position of the galactose moiety of lactose. This latter activity, although it may sometime compromise the purity of a desired product in a particular biosynthesis, can also sometimes be advantageous, leading to the production of useful oligosaccharides as side products. The compositions and methods described herein overcomes these problems by providing novel α(1,3) fucosyltransferases, which generate higher 3-fucosyllactose yields, enable the production of LNF-III, and/or possess properties leading to either enhanced or reduced levels of oligosaccharide side products. The novel α(1,3) fucosyltransferases of the present invention therefore provide advantages over known α(1,3) fucosyltransferases, including FutA.
FutA: SEQ ID NO: 54
Identification of Alternative α(1,3) Fucosyltransferases
To identify novel α(1,3)fucosyltransferases, two sequential database screens were performed. An outline of these two sequential screens is shown in
First, the sequence of a single known lactose-accepting α(1,3)fucosyltransferase (i.e. H. pylori strain 26695 FutA) was used to search public databases to find simple homologs that might represent additional lactose-accepting α(1,3)fucosyltransferases. The amino acid sequence of FutA was used as a query in the search algorithm PSI-BLAST (Position Specific Iterated Basic Local Alignment Search Tool) in order to identify novel α(1,3) FTs. The PSI-BLAST program, using a given query protein sequence, generates a list of closely related proteins sequences based on a homology search of a database. These protein homolog hits are then used by the program to generate a profile reflecting their sequence similarities to the original query. The profile is then used by the algorithm to identify an expanded group of homolog proteins, and the process is iterated several times until the number of additional new candidates obtained after each iteration decreases. (Altschul et al., 1990, J. Mol. Bio. 215:403-410; Altschul et al., 1997, Nucleic Acids Res. 25:3389-3402).
The FutA amino acid sequence was used as a query for 3 iterations of the PSI-BLAST search algorithm. This approach yielded a group of 500 candidates with similarity to FutA, many of which were highly related to FutA (shared amino acid identity in the range of 50-90%) as well as a group that was more distantly related (shared amino acid identity less than 50%). Of note, FutA produces sub-optimal yields of 3FL when used in a metabolically engineered E. coli production strain. In addition, production of FutA appears to be moderately toxic in certain E. coli production strains, including the preferred strain for use herein. Therefore, candidates for further analysis were targeted from the more distantly related group identified via the PSI-BLAST search (shared amino acid identity to FutA of less than 50%) (Table 1). This group of candidates was similar to FutA, but primarily within the catalytic domain region of the respective proteins (Martin, S. L., et al. (1997). Lewis X biosynthesis in Helicobacter pylori. Molecular cloning of an alpha(1,3)-fucosyltransferase gene. J Biol Chem 272, 21349-356.; Breton, C., et al. (1998). Conserved structural features in eukaryotic and prokaryotic fucosyltransferases. Glycobiology 8, 87-94.; Rasko, A. (2000). Cloning and Characterization of the alpha (1,3/4) Fucosyltransferase of Helicobacter pylori. Journal of Biological Chemistry 275, 4988-994.). It is preferred that the α(1,3) fucosyltransferase of the invention, sharing 50% or less, preferably 45% or less, more preferably 42% or less overall sequence identity with FutA, at the same time possess a higher level of localized sequence identity to FutA within the catalytic domain (i.e. the regions covered by the thick black bars in
These more distantly related (less than 50% sequence identity to FutA) predicted α(1,3) fucoysyl transferases (FTs) were further screened to identify predicted α(1,3) FTs from bacterial species that incorporate fucose into the 0-antigen of their lipopolysaccharide (LPS) or into the polysaccharide subunits that compose the cell surface capsule. Predicted α(1,3) FTs from these types of organisms are more likely to utilize fucose as a substrate, given the presence of fucose in their surface carbohydrate structures. Predicted α(1,3) FTs from known enteric bacterial species, either commensals or pathogens, were also identified. Such organisms sometimes display carbohydrate structures on their cell-surface that contain fucose and mimic various 3-fucosyl containing Lewis antigen structures found in higher organisms (Coyne, M. J., et al. (2005). Human symbionts use a host-like pathway for surface fucosylation. Science 307, 1778-781.; Appelmelk, B. J., et al. (1998). Phase variation in Helicobacter pylori lipopolysaccharide. Infect Immun 66, 70-76.; Ma, B., et al. (2006). Fucosylation in prokaryotes and eukaryotes. Glycobiology 16, 158R-184R.). Again, candidate α(1,3) FTs from these types of organisms are believed to be more likely to utilize fucose as a substrate and also to catalyze the linkage of fucose to useful acceptor oligosaccharides.
11 predicted α(1,3) FTs with homology to FutA ranging from 6-42% at the amino acid level were identified from PSI-BLAST. All of these candidates are found in bacteria that are known to interact with the gastrointestinal system of higher organisms. In addition, 3 of these candidates are found in bacteria that have been shown to incorporate fucose into their cell surface glycans. For ease of description, the genes encoding these proteins were named cafA-K for candidate alpha (1,3) fucosyltransferase. The caf genes were cloned by standard molecular biological techniques into an expression plasmid.
This plasmid utilizes the strong leftwards promoter of bacteriophage λ (termed PL) to direct expression of the candidate genes (Sanger, F., 1982, J. Mol. Bio. 162:729-773). The promoter is controllable, e.g., a trp-cI construct is stably integrated the into the E. coli host's genome (at the ampC locus), and control is implemented by adding tryptophan to the growth media. Gradual induction of protein expression is accomplished using a temperature sensitive cI repressor. Another similar control strategy (temperature independent expression system) has been described (Mieschendahl et al., 1986, Bio/Technology 4:802-808). The plasmid also carries the E. coli rcsA gene to up-regulate GDP-fucose synthesis, a critical precursor for the synthesis of fucosyl-linked oligosaccharides. In addition, the plasmid carries a β-lactamase (bla) gene for maintaining the plasmid in host strains by ampicillin selection (for convenience in the laboratory) and a native thyA (thymidylate synthase) gene as an alternative means of selection in thyA− hosts. Alternative selectable markers include the proBA genes to complement proline auxotrophy (Stein et al., (1984), J Bacteriol 158:2, 696-700 (1984) or purA to complement adenine auxotrophy (S. A. Wolfe, J. M. Smith, J Biol Chem 263, 19147-53 (1988)). To act as plasmid selectable markers each of these genes are first inactivated in the host cell chromosome, then wild type copies of the genes are provided on the plasmid. Alternatively a drug resistance gene may be used on the plasmid, e.g. beta-lactamase (this gene is already on the expression plasmid described above, thereby permitting selection with ampicillin). Ampicillin selection is well known in the art and described in standard manuals such as Maniatis et al., (1982) Molecular cloning, a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring, N.Y.
The expression constructs were transformed into a host strain useful for the production of fucosylated oligosaccharides and the ability to direct the production of 3FL using lactose as an acceptor sugar was assessed. Candidate α(1,3) FTs CafC (SEQ ID NO: 2), CafF (SEQ ID NO: 1), CafA (SEQ ID NO: 4) and CafB (SEQ ID NO: 5) were found to be lactose-utilizing α(1,3) fucosyltransferases. (See Table 1 and
H. pylori 26695
B. fragilis NCTC
B. fragilis NCTC
B. nordii
H. hepaticus ATCC
H. hepaticus ATCC
A. muciniphilia
H. bilis ATCC
H. cinaedi ATCC
H. bizzozeronii
R. bellii RML369-C
V. cholerae MO45
1In combination with the α(1,2) fucosyltransferase WbgL (accession no. ADN43847.1)
2In combination with the β(1,3) N-acetylglucosaminyltransferase LgtA (N. meningitidis MC58, accession no. NP_274923.1) and the β(1,4) galactosyltransferase HP0826 (H. pylori 26695, accession no. NP_207619.1)
The second database screen to identify additional novel α(1,3)fucosyltransferases was then performed. A multiple sequence alignment was generated using the two strongest previously identified lactose-utilizing α(1,3)fucosyltransferase protein sequences from the first screen: i.e. CafC and CafF. The sequence alignment and percentage of sequence identity of these two sequences is shown in Table 2 below.
Akker-
mansia
mucini-
phila
Bacter-
oides
nordii
Akker-
mansia
mucini-
phila
Bacter-
oides
nordii
Akker-
mansia
mucini-
phila
Bacter-
oides
nordii
Akker-
mansia
mucini-
phila
Bacter-
oides
nordii
Akker-
mansia
mucini-
phila
Bacter-
oides
nordii
A second iterative PSI-BLAST screen was then performed, this time using the FASTA-formatted CafC and CafF multiple sequence alignment as the query, with the NCBI PSI-BLAST program run on a local copy of NCBI BLAST+ version 2.2.29. An initial position-specific scoring matrix file (.pssm) was generated by PSI-BLAST, which the program then used to adjust the score of iterative homology search runs. The process is iterated to generate an even larger group of candidates, and the results of each run were used to further refine the matrix.
This PSI-BLAST search resulted in an initial 2586 hits. There were 996 hits with greater than 25% sequence identity to CafF. 87 hits were of greater than 250 amino acids in length. Additional analysis of the hits was performed, including comparing the sequences by BLAST to the existing inventory of known α(1,3) fucosyltransferases, (i.e. FutA, CafC, CafF, CafA and CafB), and manual annotation of hit sequences to identify those hits originating from bacteria that naturally exist in the gastrointestinal tract, as well as to remove eukaryotic and “pylori” sequences and duplicates. An annotated list of the novel α(1,3) fucosyltransferases identified by this screen (and subsequent filtering) are listed in Table 5. Table 5 provides the bacterial species from which the candidate enzyme is found, the GenBank Accession Number, GI Identification Number, amino acid sequence, and % sequence identity to CafF.
Akkermansia
muciniphila;
Akkermansia
muciniphila
muciniphila
Bacteroides
nordii;
Bacteroides
nordii
nordii]
Bacteroides
fragilis;
Bacteroides
fragilis
fragilis NCTC
Bacteroides
fragilis;
Bacteroides
fragilis
fragilis]
Helicobacter
cinaedi;
Helicobacter
cinaedi
cinaedi]
Butyrivibrio
fibrisolvens
fibrisolvens]
Butyrivibrio
Parabac-
teroides
goldsteinii;
Parabac-
teroides
teroides
goldsteinii]
goldsteinii
Tannerella
Lachno-
spiraceae
bacterium
spiraceae
bacterium
Methano-
brevibacter
ruminantium;
Methano-
brevibacter
brevibacter
ruminantium
ruminantium
Bacteroides
salyersiae;
Bacteroides
salyersiae
salyersiae]
Lachno-
spiraceae
bacterium
spiraceae
bacterium
Parabac-
teroides;
Parabac-
teroides
bacteroides]
goldsteinii
Parabac-
teroides
Clostridium
bolteae;
Clostridium
bolteae 90A9;
bolteae]
Clostridium
bolteae 90133;
Clostridium
bolteae 90138
Helicobacter
canis NCTC
canis NCTC
Helicobacter
canis NCTC
canis NCTC
Akkermansia
Gillisia
limnaea;
Gillisia
limnaea
limnaea]
Loktanella
vestfoldensis
vestfoldensis]
Azospirillum
brasilense;
Azospirillum
brasilense
brasilense
Lachno-
spiraceae
bacterium
spiraceae
bacterium
Butyrivibrio
Anaeromyxo-
bacter
dehalogenans;
Anaeromyxo-
bacter
dehalogenans
bacter
dehalogenans
Azospirillum
lipoferum;
Azospirillum
Algoriphagus
machipon-
gonensis]
Helicobacter
canis NCTC
canis NCTC
Anaeromyxo-
bacter
dehalogenans;
Anaeromyxo-
bacter
dehalogenans
bacter
dehalogenans
Coralio-
margarita
akajimensis;
Coralio-
margarita
margarita
akajimensis
akajimensis
Helicobacter
fennelliae
fennelliae
Prevotella
Flavobacterium
bacterium
Flavobacterium
bacterium
Butyrivibrio
Helicobacter
pylori;
Helicobacter
pylori
pylori]
Verrucomicrobia
bacterium
microbia
bacterium
Clostridium
citroniae;
Clostridium
citroniae
citroniae]
Helicobacter
bilis;
Helicobacter
bilis
bilis]
Verrucomicrobia
bacterium
microbia
bacterium
Moumouvirus
goulette
goulette]
Of the identified hits, 12 novel α(1,3) fucosyltransferases were further analyzed for their functional capacity: Butyrivibrio fibrisolvens CafK, Butyrivibri sp. CafL, Parabacteroides goldsteinii CafM, Tannerella sp. CafN, Lachnospiracae bacterium CafO, Methanobrevibacter ruminantium CafP, Bacteroides salyersiae CafQ, Lachnospiraceae bacterium CafR, Parabacteroides goldsteinii CafS, Clostridium bolteae CafT, Helicobacter canis CafU and Helicobacter canis CafV.
The sequence identity between the 12 novel α(1,3) fucosyltransferases identified in this second screen, the previously identified lactose-utilizing α(1,3) fucosyltransferases from the first screen, and FutA is shown in Tables 2 and 3 below.
Bacteroides nordii CafC
Akkermansia muciniphila CafF
Helicobacter pylori FutA
Tannerella sp. CafN
Bacteroides fragilis CafA
Bacteroides salyersiae CafQ
Parabacteroides goldsteinii CafS
Clostridium bolteae CafT
Methanobrevibacter ruminantium CafP
Lachnospiraceae bacterium CafO
Lachnospiraceae bacterium CafR
Butyrivibrio sp. CafL
Helicobacter canis CafU
Helicobacter canis CafV
Butyrivibrio fibrisolvens CafK
Parabacteroides goldsteinii CafM
Bacteroides fragilis CafB
Helicobacter hepaticus CafD
Bacteroides nordii CafC
Akkermansia muciniphila CafF
Helicobacter pylori FutA
Tannerella sp. CafN
Bacteroides fragilis CafA
Bacteroides salyersiae CafQ
Parabacteroides goldsteinii CafS
Clostridium bolteae CafT
Methanobrevibacter ruminantium CafP
Lachnospiraceae bacterium CafO
Lachnospiraceae bacterium CafR
Butyrivibrio sp. CafL
Helicobacter canis CafU
Helicobacter canis CafV
Butyrivibrio fibrisolvens CafK
Parabacteroides goldsteinii CafM
Bacteroides fragilis CafB
Helicobacter hepaticus CafD
Bacteroides nordii CafC
Akkermansia muciniphila
Helicobacter pylori FutA
Tannerells sp. CafN
Bacteroides fragilis
Bacteroides salyersiae
Parabacteroides
goldsteinii CafS
Clostridium bolteae
Methanobrevibacter
ruminantium CafP
Lachnospiraceae
bacterium CafO
Lachnospiraceae
bacterium CafR
Butyrivibrio sp. CafL
Heliobacter canis CafU
Heliobacter canis CafV
Butyrivibrio
fibrisolvens CafK
Parabacteroides
goldsteinii CafM
Bacteroides fragilis
Helicobacter hepaticus
Bacteroides nordii CafC
Akkermansia muciniphila
Heliobacter pylori FutA
Tannerella sp. CafN
Bacteroides fragilis
Bacteroides salyersiae
Parabacteroides
goldsteinii CafS
Clostridium boltreae
Methanobrevibacter
ruminantium CafP
Lachnospiraceae
bacterium CafO
Lachnospiraceae
bacterium CafR
Butyrivibrio sp. CafL
Helicobacter canis CafU
Helicobacter canis CafV
Butyrivibrio
fibrisolvens CafK
Parabacteroides
goldsteinii CafM
Bacteroides fragilis
Helicobacter hepaticus
Bacteroides nordii CafC
Akkermansia muciniphila
Helicobacter pylori FutA
Tannerella sp. CafN
Bacteroides fragilis
Bacteroides salyersiae
Parabacteroides
goldsteinii CafS
Clostridium bolteae CafT
Methanobrevibacter
ruminantium CafP
Lachnospiraceae
bacterium CafO
Lachnospiraceae
bacterium CafR
Butyrivibrio sp. CafL
Helicobacter canis CafU
Helicobacter canis CafV
Butyrivibrio
fibrosolvens CafK
Parabacteroides
goldsteinii CafM
Bacteroides fragilis
Helicobacter hepaticus
Bacteroides nordii CafC
Akkermansia muciniphila
Helicobacter pylori FutA
Tannerella sp. CafN
Bacteroides fragilis
Bacteroides salyersiae
Parabacteroides
goldsteinii CafS
Clostridium bolteae CafT
Methanobrevibacter
ruminantium CafP
Lachnospirceae
bacterium CafO
Lachnospirceae
bacterium CafR
Butyrivibrio sp. CafL
Helicobacter canis CafU
Helicobacter canis CafV
Butyrivibrio
fibrosolvens CafK
Parabacteroides
goldsteinii CafM
Bacteroides fragilis
Helicobacter hepaticus
Bacteroides nordii CafC (SEQ ID NO: 2)
Akkermansia muciniphila CafF (SEQ ID NO: 1)
Helicobacter pylori FutA (SEQ ID NO: 54)
Tannerrella sp. CafN (SEQ ID NO: 9)
Bacteroides fragilis CafA (SEQ ID NO: 3)
Bacteroides Salyersiae CafQ (SEQ ID NO: 12)
Parabacteroides golsteinii CafS (SEQ ID NO: 14)
Clostridium bolteae CafT (SEQ ID NO: 15)
Methanobrevibacter ruminantium CafP (SEQ ID NO: 11)
Lachnospiraceae bacterium CAfO (SEQ ID NO: 10)
Lachnospiraceae bacterium CafR (SEQ ID NO: 13)
Butyrivibrio sp. CafL (SEQ ID NO: 7)
Helicobacter Canis CafU (SEQ ID NO: 16)
Helicobacter Canis CafV (SEQ ID NO: 17)
Butyrivibrio fibrosolvens CafK (SEQ ID NO: 6)
Parabacteroides goldsteinii CafM (SEQ ID NO: 8)
Bacteroides fragilis CafB (SEQ ID NO: 4)
Helicobacter hepaticus CafD (SEQ ID NO: 53)
Based on the amino acid sequences of the identified α(1,3) fucosyltransferases (i.e., in Table 5), synthetic genes are designed and constructed by the skilled artisan using standard methods known in the art. For example, the synthetic genes include a ribosomal binding site, are codon-optimized for expression in a host bacterial production strain (i.e., E. coli), and have common 6-cutter restriction sites or sites recognized by endogenous restriction enzymes present in the host strain (i.e., EcoK restriction sites) removed to ease cloning and expression in the E. coli host strain. In a preferred embodiment, the synthetic genes are constructed with the following configuration: EcoRI site—T7g10 RBS—α(1,3) FT synthetic gene—XhoI site.
The nucleic acid sequences of sample synthetic genes for the 12 identified α(1,3) fucosyltransferases are shown in Table 6. Start and stop codons are underlined and bolded
AG
TAGCTCGAGTGACTGACTG
In any of the methods described herein, the α(1,3) fucosyltransferase genes or gene products may be variants or functional fragments thereof. A variant of any of genes or gene products disclosed herein may have 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% sequence identity to the nucleic acid or amino acid sequences described herein.
Variants as disclosed herein also include homolog, orthologs, or paralogs of the genes or gene products described herein that retain the same biological function as the genes or gene products specified herein. These variants can be used interchangeably with the genes recited in these methods. Such variants may demonstrate a percentage of homology or identity, for example, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% identity conserved domains important for biological function, preferably in a functional domain, e.g. catalytic domain.
The term “% identity,” in the context of two or more nucleic acid or polypeptide sequences, refer to two or more sequences or subsequences that are the same or have a specified percentage of amino acid residues or nucleotides that are the same, when compared and aligned for maximum correspondence, as measured using one of the following sequence comparison algorithms or by visual inspection. For example, % identity is relative to the entire length of the coding regions of the sequences being compared, or the length of a particular fragment or functional domain thereof.
For sequence comparison, typically one sequence acts as a reference sequence, to which test sequences are compared. When using a sequence comparison algorithm, test and reference sequences are input into a computer, subsequence coordinates are designated, if necessary, and sequence algorithm program parameters are designated. The sequence comparison algorithm then calculates the percent sequence identity for the test sequence(s) relative to the reference sequence, based on the designated program parameters.
Percent identity is determined using search algorithms such as BLAST and PSI-BLAST (Altschul et al., 1990, J Mol Biol 215:3, 403-410; Altschul et al., 1997, Nucleic Acids Res 25:17, 3389-402). For the PSI-BLAST search, the following exemplary parameters are employed: (1) Expect threshold was 10; (2) Gap cost was Existence:11 and Extension:1;
(3) The Matrix employed was BLOSUM62; (4) The filter for low complexity regions was “on”.
The three dimensional structure of the lactose-utilizing α(1,3) fucosyltransferase Helicobacter pylori FutA (FucT) is described in H. Y. Sun, S. W. Lin, T. P. Ko, J. F. Pan, et al., J Biol Chem 282, 9973-82 (2007). Here the amino acid residues essential for the substrate binding and the catalytic mechanism of the enzyme are discussed—in particular the sequences lying between FutA residues 31-42 (substrate binding), 85-129 (active site region 1) and 180-266 (active site region 2), with specific amino acid residues E96, R196, E250 and K251 are involved in catalysis.
Changes are introduced by mutation into the nucleic acid sequence or amino acid sequence of any of the genes or gene products described herein, leading to changes in the amino acid sequence of the encoded protein or enzyme, without altering the functional ability of the protein or enzyme. For example, nucleotide substitutions leading to amino acid substitutions at “non-essential” amino acid residues can be made in the sequence of any of sequences expressly disclosed herein. A “non-essential” amino acid residue is a residue at a position in the sequence that can be altered from the wild-type sequence of the polypeptide without altering the biological activity, whereas an “essential” amino acid residue is a residue at a position that is required for biological activity. For example, amino acid residues that are conserved among members of a family of proteins are not likely to be amenable to mutation. Other amino acid residues, however, (e.g., those that are poorly conserved among members of the protein family) may not be as essential for activity and thus are more likely to be amenable to alteration. Thus, another aspect of the invention pertains to nucleic acid molecules encoding the proteins or enzymes disclosed herein that contain changes in amino acid residues relative to the amino acid sequences disclosed herein that are not essential for activity (i.e., fucosyltransferase activity). Preferably, at least 0.1% of the activity of the reference enzyme is retained. In some embodiments, low α1,3 fucosyltransferase activity enzymes may be used in the production of large quantities of 3FL. For example, CafC is expressed very well in E. coli, leading to the easy generation of a vast excess of α1,3 fucosyltransferase enzymatic activity over that required for the production of large amounts of 3FL. Thus even variants of CafC enzyme with a relatively low level (e.g., 0.1, 1, 10%) of activity relative to the wildtype CafC enzyme, may produce useful levels of the product, 3FL.
An isolated nucleic acid molecule encoding a protein essentially retaining the functional capability compared to any of the genes described herein can be created by introducing one or more nucleotide substitutions, additions or deletions into the corresponding nucleotide sequence, such that one or more amino acid substitutions, additions or deletions are introduced into the encoded protein.
Mutations are introduced into a nucleic acid sequence by standard techniques such that the encoded amino acid sequence is altered, such as site-directed mutagenesis and PCR-mediated mutagenesis. Preferably, conservative amino acid substitutions are made at one or more predicted non-essential amino acid residues. A “conservative amino acid substitution” is one in which the amino acid residue is replaced with an amino acid residue having a similar side chain. Families of amino acid residues having similar side chains have been defined in the art. Certain amino acids have side chains with more than one classifiable characteristic. These families include amino acids with basic side chains (e.g., lysine, arginine, histidine), acidic side chains (e.g., aspartic acid, glutamic acid), uncharged polar side chains (e.g., glycine, asparagine, glutamine, serine, threonine, tyrosine, tryptophan, cysteine), nonpolar side chains (e.g., alanine, valine, leucine, isoleucine, proline, phenylalanine, methionine, tyrosine, tryptophan), beta-branched side chains (e.g., threonine, valine, isoleucine) and aromatic side chains (e.g., tyrosine, phenylalanine, tryptophan, histidine). Thus, a predicted nonessential amino acid residue in a given polypeptide is replaced with another amino acid residue from the same side chain family. Alternatively, in another embodiment, mutations can be introduced randomly along all or part of a given coding sequence, such as by saturation mutagenesis, and the resultant mutants can be screened for given polypeptide biological activity to identify mutants that retain activity. Conversely, the invention also provides for variants with mutations that enhance or increase the endogenous biological activity. Following mutagenesis of the nucleic acid sequence, the encoded protein can be expressed by any recombinant technology known in the art and the activity of the protein can be determined. An increase, decrease, or elimination of a given biological activity of the variants disclosed herein can be readily measured by the ordinary person skilled in the art, i.e., by measuring the capability for mediating oligosaccharide modification, synthesis, or degradation (via detection of the products).
The present invention includes functional fragments of the genes or gene products described herein, e.g., catalytic domain portions of the enzyme shown in
For example, fragments of any of the proteins or enzymes disclosed herein or encoded by any of the genes disclosed herein can be 10 to 20 amino acids, 10 to 30 amino acids, 10 to 40 amino acids, 10 to 50 amino acids, 10 to 60 amino acids, 10 to 70 amino acids, 10 to 80 amino acids, 10 to 90 amino acids, 10 to 100 amino acids, 50 to 100 amino acids, 75 to 125 amino acids, 100 to 150 amino acids, 150 to 200 amino acids, 200 to 250 amino acids, 250 to 300 amino acids, 300 to 350 amino acids, 350 to 400 amino acids, 400 to 450 amino acids, or 450 to 500 amino acids. The fragments encompassed in the present invention comprise fragments that retain functional fragments. As such, the fragments preferably retain the catalytic domains that are required or are important for functional activity. Fragments can be determined or generated by using the sequence information herein, and the fragments can be tested for functional activity using standard methods known in the art. For example, the encoded protein can be expressed by any recombinant technology known in the art and the activity of the protein can be determined. The biological function of said fragment can be measured by measuring ability to synthesize or modify a substrate oligosaccharide, or conversely, to catabolize an oligosaccharide substrate.
Within the context of the invention, “functionally equivalent”, as used herein, refers to a gene or the resulting encoded protein variant or fragment thereof capable of exhibiting a substantially similar activity as the wild-type fucosyltransferase. Specifically, the fucosyltransferase activity refers to the ability to transfer a fucose sugar to an acceptor substrate via an alpha-(1,3)-linkage. As used herein, “substantially similar activity” refers to an activity level within 5%, 10%, 20%, 30%, 40%, or 50% of the wild-type fucosyltransferase.
To test for lactose-utilizing fucosyltransferase activity, the production of α(1,3) fucosylated oligosaccharides is evaluated in a host organism that expresses a candidate enzyme synthetic gene and which contains both cytoplasmic GDP-fucose and lactose pools. The production of fucosylated oligosaccharides indicates that the candidate enzyme-encoding sequence functions as a lactose-utilizing α(1,3)fucosyltransferase.
The invention also provides nucleic acid constructs (i.e., a plasmid or vector) carrying the nucleic acid sequence of a novel α(1,3) fucosyltransferases for the expression of the novel α(1,3) fucosyltransferases in host bacterium.
The invention also provides methods for producing fucosylated oligosaccharides by expressing the novel α(1,3) fucosyltransferases in suitable host production bacterium, as further described herein.
Engineering of E. coli to Produce α(1,3) Fucosylated Human Milk Oligosaccharide
Described herein is a gene screening approach, which was used to validate the novel α(1,3) fucosyltransferases (α(1,3) FTs) for the synthesis of fucosyl-linked oligosaccharides in metabolically engineered E. coli. Of particular interest are α(1,3) FTs that are capable of the synthesis of the HMOS 3-fucosyllactose (3-FL), lactodifucotetraose (LDFT), or lacto-N-fucopentaose III (LNF III). Of most interest are α(1,3) FTs that catalyze the synthesis of 3-FL. Preferably, the α(1,3) fucosyl-linked oligosaccharides are expressed in metabolically engineered E. coli.
In particular, therefore, the invention provides α(1,3) FTs that are capable of the synthesis of the HMO (human milk oligosaccharide) 3-fucosyllactose (3FL). As explained above, 3FL is one of the most abundant fucosylated oligosaccharide present in human milk, and is thought to function with other HMOS to promote the growth of beneficial commensal bacteria in the infant gut.
Production Host Strains
A suitable production host strain is one that is not the same bacterial strain as the source bacterial strain from which the fucosyltransferase-encoding nucleic acid sequence was identified.
E. coli K-12 is a well-studied bacterium which has been the subject of extensive research in microbial physiology and genetics and commercially exploited for a variety of industrial uses. The natural habitat of the parent species, E. coli, is the large bowel of mammals. E. coli K-12 has a history of safe use, and its derivatives are used in a large number of industrial applications, including the production of chemicals and drugs for human administration and consumption. E. coli K-12 was originally isolated from a convalescent diphtheria patient in 1922. Because it lacks virulence characteristics, grows readily on common laboratory media, and has been used extensively for microbial physiology and genetics research, it has become the standard bacteriological strain used in microbiological research, teaching, and production of products for industry and medicine. E. coli K-12 is now considered an enfeebled organism as a result of being maintained in the laboratory environment for over 70 years. As a result, K-12 strains are unable to colonize the intestines of humans and other animals under normal conditions. Additional information on this well-known strain is available at http://epa.gov/oppt/biotech/pubs/fra/fra004.htm. In addition to E. coli K-12, other bacterial strains are used as production host strains, e.g., a variety of bacterial species may be used in the oligosaccharide biosynthesis methods, e.g., Erwinia herbicola (Pantoea agglomerans), Citrobacter freundii, Pantoea citrea, Pectobacterium carotovorum, or Xanthomonas campestris. Bacteria of the genus Bacillus may also be used, including Bacillus subtilis, Bacillus licheniformis, Bacillus coagulans, Bacillus thermophilus, Bacillus laterosporus, Bacillus megaterium, Bacillus mycoides, Bacillus pumilus, Bacillus lentus, Bacillus cereus, and Bacillus circulans. Similarly, bacteria of the genera Lactobacillus and Lactococcus may be modified using the methods of this invention, including but not limited to Lactobacillus acidophilus, Lactobacillus salivarius, Lactobacillus plantarum, Lactobacillus helveticus, Lactobacillus delbrueckii, Lactobacillus rhamnosus, Lactobacillus bulgaricus, Lactobacillus crispatus, Lactobacillus gasseri, Lactobacillus casei, Lactobacillus reuteri, Lactobacillus jensenii, and Lactococcus lactis. Streptococcus thermophiles and Proprionibacterium freudenreichii are also suitable bacterial species for the invention described herein. Also included as part of this invention are strains, modified as described here, from the genera Enterococcus (e.g., Enterococcus faecium and Enterococcus thermophiles), Bifidobacterium (e.g., Bifidobacterium longum, Bifidobacterium infantis, and Bifidobacterium bifidum), Sporolactobacillus spp., Micromomospora spp., Micrococcus spp., Rhodococcus spp., and Pseudomonas (e.g., Pseudomonas fluorescens and Pseudomonas aeruginosa).
Suitable host strains are amenable to genetic manipulation, e.g., they maintain expression constructs, accumulate precursors of the desired end product, e.g., they maintain pools of lactose and GDP-fucose, and accumulate end product, e.g., 3FL. Such strains grow well on defined minimal media that contains simple salts and generally a single carbon source.
Biosynthesis of 3FL requires the generation of an enhanced cellular pool of both lactose and GDP-fucose (
In the Examples provided herein, the wild-type Escherichia coli K-12 prototrophic strain W3110 was selected as the parent background host to test the ability of the candidates to catalyze 3FL production (Bachmann, 1972). The particular W3110 derivative employed was one that previously had been modified by the introduction (at the ampC locus) of a tryptophan-inducible PtrpB CI+ repressor cassette, generating an E. coli strain known as GI724 (LaVallie et al., 2000). Other features of GI724 include lacIq and lacPL8 promoter mutations. E. coli strain GI724 affords economical production of recombinant proteins from the phage X PL promoter following induction with low levels of exogenous tryptophan (LaVallie et al., 1993; Mieschendahl et al., 1986). Additional genetic alterations (described below) were made to this strain to promote the biosynthesis of 3FL. This was achieved in strain GI724 through several manipulations of the chromosome using λ Red recombineering (Court et al., 2002) and generalized P1 phage transduction.
First: the ability of the E. coli host strain to accumulate intracellular lactose was engineered by simultaneous deletion of the endogenous β-galactosidase gene (lacZ) and the lactose operon repressor gene (lacI). During construction of this deletion the lacIq promoter was placed immediately upstream of the lactose permease gene, lacY. The strain thus modified maintains its ability to transport lactose from the culture medium (via LacY), but is deleted for the wild-type copy of the lacZ (β-galactosidase) gene responsible for lactose catabolism. An intracellular lactose pool is therefore created when the modified strain is cultured in the presence of exogenous lactose. In addition, the lacA gene was deleted in order to eliminate the production of acetyl-lactose from the enhanced pool of intracellular lactose.
Second: the ability of the host E. coli strain to synthesize colanic acid, an extracellular capsular polysaccharide, was eliminated by the deletion of the wcaJ gene, encoding the UDP-glucose lipid carrier transferase (Stevenson et al., 1996). In a wcaJ null background GDP-fucose accumulates in the E. coli cytoplasm (Dumon, C., et al. (2001). In vivo fucosylation of lacto-N-neotetraose and lacto-N-neohexaose by heterologous expression of Helicobacter pylori alpha-1,3 fucosyltransferase in engineered Escherichia coli. Glycoconj J 18, 465-474.)
The sequence of the chromosomal region of E. coli bearing the ΔwcaJ::FRT mutation is set forth below (SEQ ID NO: 55):
Third: The magnitude of the cytoplasmic GDP-fucose pool was enhanced by the introduction of a null mutation into the lon gene. Lon is an ATP-dependent intracellular protease that has been shown to be responsible for degrading RcsA, a positive transcriptional regulator of colanic acid biosynthesis in E. coli (Gottesman and Stout, 1991). In a lon null background RcsA is stabilized, RcsA levels increase, the genes responsible for GDP-fucose synthesis are up-regulated, and intracellular GDP-fucose concentrations are enhanced. The lon gene was almost entirely deleted in our production strain (E638) and replaced by an inserted functional, wild-type, but promoter-less E. coli lacZ+ gene (Δlon::(kan, lacZ+). λ Red recombineering was used to perform the construction.
Genomic DNA sequence surrounding the lacZ+ insertion into the lon region in the E. coli strain is set forth below (SEQ ID NO: 56):
The inserted lacZ+ cassette not only knocks out lon, but also converts the lacZ− host back to both a lacZ+ genotype and phenotype. The modified strain produces a minimal (albeit still readily detectable) level of β-galactosidase activity (1-2 units), which has very little impact on lactose consumption during production runs, but which is useful in removing residual lactose at the end of runs, and as an easily scorable phenotypic marker for moving the lon mutation into other lacZ− E. coli strains by P1 transduction.
Fourth: A thyA (thymidylate synthase) mutation was introduced into the strain by P1 transduction. In the absence of exogenous thymidine, thyA strains are unable to make DNA and die. The defect can be complemented in trans by supplying a wild-type thyA gene on a multicopy plasmid (Belfort et al., 1983). This complementation is used here as a means of plasmid maintenance.
An additional modification that is useful for increasing the cytoplasmic pool of free lactose (and hence the final yield of 3-FL) is the incorporation of a lacA mutation. LacA is a lactose acetyltransferase that is only active when high levels of lactose accumulate in the E. coli cytoplasm. High intracellular osmolarity (e.g., caused by a high intracellular lactose pool) can inhibit bacterial growth, and E. coli has evolved a mechanism for protecting itself from high intra cellular osmolarity caused by lactose by “tagging” excess intracellular lactose with an acetyl group using LacA, and then actively expelling the acetyl-lactose from the cell (Danchin, A. Bioessays 31, 769-773 (2009)). Production of acetyl-lactose in E. coli engineered to produce 3-FL or other human milk oligosaccharides is therefore undesirable: it reduces overall yield. Moreover, acetyl-lactose is a side product that complicates oligosaccharide purification schemes. The incorporation of a lacA mutation resolves these problems. Sub-optimal production of fucosylated oligosaccharides occurs in strains lacking either or both of the mutations in the colanic acid pathway and the lon protease. Diversion of lactose into a side product (acetyl-lactose) occurs in strains that do not contain the lacA mutation. A schematic of the lacA deletion and corresponding genomic sequence is provided above.
The strain used in the Examples to test the different α(1,3) FT candidates incorporates all the above genetic modifications and has the following genotype:
ΔampC::PtrpB cI, Δ(lacI-lacZ)::FRT, PlacIqlacY+, ΔlacA, ΔwcaJ::FRT, thyA::Tn10, Δlon::(npt3, lacZ+)
The strains engineered as described above to produce the desired fucosylated oligosaccharide(s) are grown in a minimal media. An exemplary minimal medium used in a bioreactor, minimal “FERM” medium, is detailed below.
Ferm (10 liters): Minimal medium comprising:
40 g (NH4)2HPO4
100 g KH2PO4
10 g MgSO4.7H2O
40 g NaOH
1× Trace elements:
1.3 g NTA (nitrilotriacetic acid)
5 g FeSO4.7H2O
0.09 g MnCl2.4H2O
0.09 g ZnSO4.7H2O
0.01 g CoCl2.6H2O
0.01 g CuCl2.2H2O
0.02 g H3BO3
0.01 g Na2MoO4.2H2O (pH 6.8)
Water to 10 liters
DF204 antifoam (0.1 ml/L)
150 g glycerol (initial batch growth), followed by fed batch mode with a 90% glycerol-1% MgSO4-1× trace elements feed, at various rates for various times.
Bacteria comprising the characteristics described herein are cultured in the presence of lactose, and a fucosylated oligosaccharide is retrieved, either from the bacterium itself or from a culture supernatant of the bacterium. The fucosylated oligosaccharide is purified for use in therapeutic or nutritional products, or the bacteria are used directly in such products.
Post-Fermentation Purification
Fucosylated oligosaccharides produced by metabolically engineered E. coli cells are purified from culture broth post-fermentation. An exemplary procedure comprises five steps. (1) Clarification: Fermentation broth is harvested and cells removed by sedimentation in a preparative centrifuge at 6000×g for 30 min. Each bioreactor run yields about 5-7 L of partially clarified supernatant. (2) Product capture on coarse carbon: A column packed with coarse carbon (Calgon 12×40 TR) of ˜1000 ml volume (dimension 5 cm diameter×60 cm length) is equilibrated with 1 column volume (CV) of water and loaded with clarified culture supernatant at a flow rate of 40 ml/min. This column has a total capacity of about 120 g of sugar. Following loading and sugar capture, the column is washed with 1.5 CV of water, then eluted with 2.5 CV of 50% ethanol or 25% isopropanol (lower concentrations of ethanol at this step (25-30%) may be sufficient for product elution.) This solvent elution step releases about 95% of the total bound sugars on the column and a small portion of the color bodies. In this first step capture of the maximal amount of sugar is the primary objective. Resolution of contaminants is not an objective. (3) Evaporation: A volume of 2.5 L of ethanol or isopropanol eluate from the capture column is rotary-evaporated at 56 C.° and a sugar syrup in water is generated. Alternative methods that could be used for this step include lyophilization or spray-drying. (4) Flash chromatography on fine carbon and ion exchange media: A column (GE Healthcare HiScale50/40, 5×40 cm, max pressure 20 bar) connected to a Biotage Isolera One FLASH Chromatography System is packed with 750 ml of a Darco Activated Carbon G60 (100-mesh): Celite 535 (coarse) 1:1 mixture (both column packings were obtained from Sigma). The column is equilibrated with 5 CV of water and loaded with sugar from step 3 (10-50 g, depending on the ratio of 3-FL to contaminating lactose), using either a celite loading cartridge or direct injection. The column is connected to an evaporative light scattering (ELSD) detector to detect peaks of eluting sugars during the chromatography. A four-step gradient of isopropanol, ethanol or methanol is run in order to separate 3-FL from monosaccharides (if present), lactose and color bodies. Fractions corresponding to sugar peaks are collected automatically in 120-ml bottles, pooled and directed to step 5. In certain purification runs from longer-than-normal fermentations, passage of the 3-FL-containing fraction through anion-exchange and cation exchange columns can remove excess protein/DNA/caramel body contaminants. Resins tested successfully for this purpose include Dowex 22.
The gene screening approach described herein was successfully utilized to identify new α(1,3) FTs for the efficient biosynthesis of 3FL and other α(1,3) fucosylated oligosaccharides in metabolically engineered E. coli host strains. The results of the screen are summarized in Tables 1 and 4.
A directed screening approach was used to identify and characterize alternative bacterial α(1,3) FTs with different and desirable properties, (e.g. possessing higher specific activity, higher expression level, lower cellular toxicity, higher protease stability and/or different acceptor substrate specificity) that are useful for the large scale production of α(1,3)-linked fucosylated oligosaccharides. Specifically, the enzymes CafC, CafL, CafN, CafO, CafQ, CafU and CafV have utility for the production of 3FL and LDFT, two HMOS that are abundant in human milk that possess important and useful therapeutic properties. In addition, CafD is capable of promoting synthesis of LNF III, an HMOS that possesses the bona fide Lex epitope that is likely to possess therapeutic properties similar to that of 3FL and LDFT. The Lex epitope is involved in a myriad of biological recognition processes, and the ability to produce molecules containing this epitope on large-scale is useful as a tool to elucidate their modes of action (McEver et al., 1995; McEver and Cummings, 1997).
The strain used to test the different α(1,3) FT candidates incorporates all the above genetic modifications and has the following genotype:
ΔampC::PtrpBcI, Δ(lacI-lacZ)::FRT, PlacIqlacY+, ΔlacA, ΔwcaJ::FRT, thyA::Tn10, Δlon::(npt3, lacZ+)
The E. coli strains harboring the different α(1,3) FT candidate expression plasmids were analyzed in small-scale experiments. Strains were grown in selective media (lacking thymidine) to early exponential phase. Lactose was then added to a final concentration of 1%, and tryptophan (200 μM) was added to induce expression of each candidate α(1,3) FT from the PL promoter. At the end of the induction period (˜20 h) equivalent OD 600 units of each strain were harvested. Lysates were prepared and analyzed for the presence of 3FL by thin layer chromatography (TLC). As shown in
In a related aspect of the invention, the bacterial production strain may harbor an expression plasmid containing two or more different α(1,3) fucosyltransferases in a “tandem” or “stringed” arrangement under control of a promoter, e.g., a fortuitous promoter. A relatively low level of constitutive expression of 2 different α(1,3) fucosyltransferases was found to yield a net increase of enzyme activity without a drawback of undesirable or unacceptable cell toxicity has been observed with high, e.g., inducible/induced, expression of a single heterologous α(1,3) fucosyltransferase. An exemplary promoter comprises the PL promoter (e.g. pG420 shown in
CafA, C and F were tested for utilization in combination with an α(1,2) fucosyltransferase produced in the same strain to catalyze the synthesis of Lactodifucotetraose (LDFT) (
The E. coli strains harboring the different LDFT expression plasmids were analyzed in small-scale experiments. Strains were grown in selective media (lacking thymidine) to early exponential phase. Lactose was then added to a final concentration of 1%, and tryptophan (200 μM) was added to induce expression of the α(1,2) and α(1,3) FTs from the PL promoter. At the end of the induction period (˜20 h) equivalent OD 600 units of each strain were harvested. Cell lysates were prepared and analyzed for the presence of intracellular LDFT by thin layer chromatography (TLC). As shown in
The majority of the α(1,3) FT candidates tested from the first database screen (CafD, E, G, H, I, J, K) were unable to utilize lactose as a donor substrate and could not promote the synthesis of 3FL, despite the fact that most of these enzymes were well-expressed in E. coli (Table 1). One explanation for this observation is that some bacterial and higher eukaryotic α(1,3) FTs prefer N-acetylglucosamine (GlcNAc) rather than glucose (Glc) as an acceptor for the attachment of fucose (Breton, C., et al. (1998). Conserved structural features in eukaryotic and prokaryotic fucosyltransferases. Glycobiology 8, 87-94.; Ma, B., et al. (2003). C-terminal amino acids of Helicobacter pylori alpha 1,3/4 fucosyltransferases determine type I and type II transfer. J Biol Chem 278, 21893-1900.; Ma, B., et al. (2006). Fucosylation in prokaryotes and eukaryotes. Glycobiology 16, 158R-184R.). Therefore, studies were carried out to determine whether CafD, E, G, H, I, J or K catalyze the attachment of fucose to a GlcNAc moiety present within the HMOS LNnT (Lacto-N-neotetraose) to generate a fucosylated oligosaccharide found in human milk termed LNF III (Lacto-N-fucopentaose) (
LgtA catalyzes the attachment of GlcNAc to the galactose in lactose to produce Lacto-N-triose (LNT2), a precursor of many HMOS that has the structure GlcNAcβ1-3Galβ1-4Glc. JHP0765 (a β(1,4) galactosyltransferase) can then utilize LNT2 as an acceptor to generate LNnT, an abundant HMOS of human milk. LNnT has the structure Galβ1-4GlcNacβ1-3Galβ1-4Glc and is an important Bifidogenic prebiotic factor in human milk (Marcobal, A., et al. (2010). Consumption of human milk oligosaccharides by gut-related microbes. J Agric Food Chem 58, 5334-340.; Garrido, D., et al. (2012). A molecular basis for bifidobacterial enrichment in the infant gastrointestinal tract. Adv Nutr 3, 415S-421S.; Sela, D. A., et al. (2012). Bifidobacterium longum subsp. infantis ATCC 15697 α-fucosidases are active on fucosylated human milk oligosaccharides. Appl Environ Microbiol 78, 795-803.). Attachment of fucose in an α1,3 linkage to the GlcNAc in LNnT generates LNF III, another HMOS found in human milk.
Derivatives of plasmid pG222 harboring each α(1,3) FT candidate were transformed into the E. coli production strain using standard techniques. The E. coli strains harboring the different LNF III expression plasmids were then analyzed in small-scale experiments. Strains were grown in selective media (lacking thymidine) to early exponential phase. Lactose was then added to a final concentration of 1%, and tryptophan (200 μM) was added to induce expression of the glycosyltransferases. At the end of the induction period (˜20 h) equivalent OD 600 units of each strain were harvested. Cell lysates were prepared and analyzed for the presence of intracellular LNF III by thin layer chromatography (TLC). As shown in
Bacterial strains were constructed that harbor an expression plasmid containing two different α(1,3) fucosyltransferases in a “tandem” arrangement or in a string (three or more genes) configuration under control of the PL promoter.
Cellular toxicity and consequent lowered product yields were observed in 3-FL bioreactor runs such strains expressing high levels of α(1,3) fucosyltransferases driven by the fully-induced PL promoter. However, by keeping the PL promoter repressed (e.g. by eliminating the addition of tryptophan to the culture and relying on the low-level of constitutive transcription that originates from the promoter region) and by constructing a tandem arrangement of the α(1,3) fucosyltransferases CafC and CafN downstream of the promoter, the culture maintains good viability for the duration of the run and 3-FL yields are significantly improved.
High level expression (e.g. as driven from the induced PL promoter) of nearly all α(1,3) fucosyltransferases tested to date can be toxic to E. coli production strains, resulting in poor viability and low 3-FL yields in fermentation runs. One explanation is that many α(1,3) fucosyltransferases may possess an off-target activity in which an endogenous E. coli molecule essential for cell viability is inappropriately fucosylated rendering it non-functional and/or toxic. Of note, some α(1,3) fucosyltransferases have been shown to use N-acetylglucosamine as an acceptor. Therefore, the identity of the secondary endogenous E. coli target may be a molecule containing N-acetylglucosamine, such as the lipid II precursor for cell wall peptidoglycan. Thus, cells producing high levels of α(1,3) fucosyltransferase activity displayed aberrant cell envelope morphology (swelling, membrane blebbing), suggesting a defect in cell wall/membrane structure or biogenesis. Interestingly, supplementation of fermentation media with a nitrogen-rich additive such as casamino acids (CAA) or yeast extract (YE) protected against the toxic properties of α(1,3) fucosyltransferase activity, leading to significantly improved 3-FL production yields. In particular, CAA supplementation increased, e.g., doubled, the yield of 3FL obtained in fermentation runs. This yield-boosting activity is associated with any rich nutritional additive containing amino acids, peptides, minerals, vitamins, and other micronutrients. In addition to CAA and YE, such additives may include any protein hydrolysate (e.g., peptone) from a variety of sources, including but not limited to meat, casein, whey, gelatin, soybean, yeast and grains.
While the invention has been described in conjunction with the detailed description thereof, the foregoing description is intended to illustrate and not limit the scope of the invention, which is defined by the scope of the appended claims. Other aspects, advantages, and modifications are within the scope of the following claims.
The patent and scientific literature referred to herein establishes the knowledge that is available to those with skill in the art. All United States patents and published or unpublished United States patent applications cited herein are incorporated by reference. All published foreign patents and patent applications cited herein are hereby incorporated by reference. Genbank and NCBI submissions indicated by accession number cited herein are hereby incorporated by reference. All other published references, documents, manuscripts and scientific literature cited herein are hereby incorporated by reference.
While this invention has been particularly shown and described with references to preferred embodiments thereof, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the scope of the invention encompassed by the appended claims.
This application is a national stage application filed under 35 U.S.C. § 371, of International Patent Application No. PCT/US2015/049257, filed Sep. 9, 2015, which claims the benefit of and priority to U.S. Provisional Application No. 62/047,851 filed on Sep. 9, 2014, the contents of all of which are incorporated herein by reference in its entirety.
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| National Center for Biotechnology Information “Predicted Glycosyl Transferas ([Escherichia coli Str. K-12 Substr. W3110)”, GenBank Accession No. BAA15898.1, Oct. 7, 2016, 13 pages. |
| National Center for Biotechnology Information “Colanic Acid Exporter [Escherichia coli str. K-12 Substr. W3110)”, GenBank Accession No. BAA15899.1, Oct. 7, 2016, 13 pages. |
| National Center for Biotechnology Information “Predicted UDP-Glucoselipid Carrier Transferase (Escherichia coli str. K-12 substr W3110)”, GenBank Accession No. BAA15900.1, Nov. 20, 2008, 13 pages. |
| National Center for Biotechnology Information “Predicted Glycosyl Transferase (Escherichia coli str. K-12 substr. W3110]”, GenBank Accession No. BAA15906.1, Oct. 7, 2016, 12 pages. |
| National Center for Biotechnology Information “Predicted Acyl Transferase (Escherichia coli str. K-12 substr. W3110)”, GenBank Accession No. BAA15910.1, Nov. 20, 2008, 13 pages. |
| National Center for Biotechnology Information “Predicted Acyl Transferase (Escherichia coli str. K-12 substr. W3110)”, GenBank Accession No. BAA15911.1, Oct. 7, 2016, 13 pages. |
| National Center for Biotechnology Information “Predicted Glycosyl Transferase (Escherichia coli str. K-12 substr. W3110]”, GenBank Accession No. BAA15912.1, Oct. 7, 2016, 13 pages. |
| National Center for Biotechnology Information “Proiein-Tyrosine kinase (Escherichia coli str. K-12 substr. W3110)”, GenBank Accession No. BAA15913.1, Oct. 7, 2016, 13 pages. |
| National Center for Biotechnology Information “wbfL [Vibrio cholerae]”, GenBank Accession No. BAA33600.1, Oct. 16, 1999, 1 page. |
| National Center for Biotechnology Information “Predicted Glycosyl Transferase (Escherichia coli str. K-12 substr. W3110)”, GenBank Accession No. BAE76572.1, Oct. 7, 2016, 13 pages. |
| National Center for Biotechnology Information “Predicted Colanic Acid Polymerase (Escherichia coli str. K-12 substr. W3110]”, GenBank Accession No. BAE76573.1, Oct. 7, 2016, 13 pages. |
| National Center for Biotechnology Information “Predicted Glycosyl Transferase (Escherichia coli str. K-12 substr. W3110]”, GenBank Accession No. BAE76574.1, Oct. 7, 2016, 13 pages. |
| National Center for Biotechnology Information “Protein-Tyrosine Phosphatase (Escherichia coli str. K-12 substr. W3110]”, GenBank Accession No. BAE76575.1, Oct. 7, 2016, 13 pages. |
| National Center for Biotechnology Information “Lipoprotein Required for Capsular Polysaccharide Translocation through the Outer Membrane (Escherichia coli str. K-12 substr. W3110)”, GenBank Accession No. BAE76576.1, Oct. 7, 2016, 13 pages. |
| National Center for Biotechnology Information “O-antigen Translocase (Escherichia coli str. K-12 substr. W3110)”, GenBank Accession No. BAE77506.1, Oct. 7, 2016, 13 pages. |
| National Center for Biotechnology Information “Bacteroides reticulotermitis JCM 10512 DNA, contig: contig00004, whole genome shotgun sequence”, GenBank Accession No. BAIV01000004.1, Sep. 15, 2015, 84 pages. |
| National Center for Biotechnology Information “putative LPS biosynthesis related glycosyltransferase [Bacteroides fragilis NCTC 9343]”, GenBank Accession No. CAH09151.1, Feb. 6, 2015, 2 pages. |
| National Center for Biotechnology Information “putative fucosyltransferase [Bacteroides fragilis NCTC 9343]”, GenBank Accession No. CAH09495.1, Feb. 6, 2015, 2 pages. |
| National Center for Biotechnology Information “DNA Sequence 01 rcsB Gene which is Regulator Gene of Capsule Polysaccharide Systhesis Gene (CPS Gene)” GenBank Accession No. E04821.1, Nov. 4, 2005, 2 pages. |
| National Center for Biotechnology Information “hypothetical protein HMPREF2087_01375, partial [Helicobacter canis NCTC 12740]”, GenBank Accession No. ETD25547.1, Dec. 10, 2013, 2 pages. |
| National Center for Biotechnology Information “hypothetical protein HMPREF2087_01719 [Helicobacter canis NCTC 12740]”, GenBank Accession No. ETD25885.1, Dec. 10, 2013, 2 pages. |
| National Center for Biotechnology Information “hypothetical protein HMPREF2087_01720 [Helicobacter canis NCTC 12740]”, GenBank Accession No. ETD25886.1, Dec. 10, 2013, 2 pages. |
| National Center for Biotechnology Information “alpha (1,3)-Fucosyltransferase [Helicobacter fennelliae MRY12-0050]”, GenBank Accession No. GAD18300.1, Sep. 15, 2015, 2 pages. |
| National Center for Biotechnology Information “E. coli ATP-dependent Protease La (Ion) Gene, Complete CDS”, GenBank Accession No. L20572.1, Mar. 17, 1994, 2 pages. |
| National Center for Biotechnology Information “Escherichia coli Capsular Polysaccharide Regulator (rcsA) Gene, Complete CDS”, GenBank Accession No. M58003.1, Dec. 6, 1995, 2 pages. |
| National Center for Biotechnology Information “Kluyveromyces Lactis Beta-D-Galactosidase (LAC4) Gene, Complete CDS”, GenBank Accession No. M84410.1, Apr. 27, 1993, 2 pages. |
| National Center for Biotechnology Information “fucosyltransferase [Helicobacter pylori 26695]”, GenBank Accession No. NP_207177.1, Aug. 2, 2016, 2 pages. |
| National Center for Biotechnology Information “Glycosyltransferase [Helicobacter Pylori 26695]”, GenBank Accession No. NP_207619.1, Aug. 2, 2016, 2 pages. |
| National Center for Biotechnology Information “Lacto-N-Neotetraose Biosynthesis Glycosyl Transferase Lgtb [Neisseria Meningitidis MC58]”, GenBank Accession No. NP_274922.1, Aug. 3, 2016, 2 pages. |
| National Center for Biotechnology Information “Lacto-N-Neotetraose Biosynthesis Glycosyl Transferase [Neisseria Meningitidis MC58]”, GenBank Accession No. NP_274923.1, Aug. 3, 2016, 2 pages. |
| National Center for Biotechnology Information “E. coli lacY Gene (Codes for Lactose Permease)”, GenBank Accession No. V00295.1, Jul. 26, 2016, 3 pages. |
| National Center for Biotechnology Information “Multispecies: hypothetical protein [Clostridiales]”, GenBank Accession No. WP_002570751.1, Sep. 4, 2018, 1 page. |
| National Center for Biotechnology Information “Alpha1,2fucosyltransferase [[Clostridium] Bolteae]”, GenBank Accession No. WP_002570768.1, Dec. 9, 2016, 1 page. |
| National Center for Biotechnology Information “alpha-1,3-fucosyl transferase [Helicobacter cinaedi]”, GenBank Accession No. WP_002956732.1, May 26, 2013, 1 page. |
| National Center for Biotechnology Information “hypothetical protein [Helicobacter bilis]”, GenBank Accession No. WP_004086382.1, May 13, 2013, 1 page. |
| National Center for Biotechnology Information “alpha-1,2-fucosyltransferase [Bacteroides caccae]”, GenBank Accession No. WP_005675707.1, Dec. 9, 2016, 1 page. |
| National Center for Biotechnology Information “hypothetical protein [Bacteroides salyersiae]”, GenBank Accession No. WP_005934126.1, Jun. 4, 2013, 1 page. |
| National Center for Biotechnology Information “putative LPS biosynthesis related glycosyltransferase [Gillisia limnaea]”, GenBank Accession No. WP_006987752.1, Jun. 4, 2013, 1 page. |
| National Center for Biotechnology Information “glycosyl transferase [Bacteroides nordii]”, GenBank Accession No. WP_007483358.1, Dec. 7, 2015, 1 page. |
| National Center for Biotechnology Information “hypothetical protein [Parabacteroides goldsteinii]”, GenBank Accession No. WP_007657871.1, Jun. 5, 2013, 1 page. |
| National Center for Biotechnology Information “hypothetical protein [[Clostridium] citroniae]”, GenBank Accession No. WP_007869439.1, Jun. 5, 2013, 1 page. |
| National Center for Biotechnology Information “Alpha-1,2-Fucosyltransferase [Parabacteroides Johnsonii]”, GenBank Accession No. WP_008155883.1, Dec. 9, 2016, 1 page. |
| National Center for Biotechnology Information “alpha (1,3)-fucosyltransferase [Algoriphagus machipongonensis]”, GenBank Accession No. WP_008200114.1, Jun. 5, 2013, 1 page. |
| National Center for Biotechnology Information “Multispecies: Alpha-1,2-Fucosyltransferase [Clostridiales]”, GenBank Accession No. WP_009251343.1, Apr. 19, 2017, 1 page. |
| National Center for Biotechnology Information “Multispecies: hypothetical protein [Parabacteroides]”, GenBank Accession No. WP_010803436.1, May 13, 2013, 1 page. |
| National Center for Biotechnology Information “hypothetical protein, partial [Flavobacterium sp. ACAM 123]”, GenBank Accession No. WP_016989022.1, Jun. 27, 2013, 1 page. |
| National Center for Biotechnology Information “hypothetical protein [Flavobacterium sp. ACAM 123]”, GenBank Accession No. WP_016991062.1, Jun. 27, 2013, 1 page. |
| National Center for Biotechnology Information “hypothetical protein [Yoonia vestfoldensis]”, GenBank Accession No. WP_019955165.1, Jun. 30, 2013, 1 page. |
| National Center for Biotechnology Information “hypothetical protein [Verrucomicrobia bacterium SCGC AAA300-K03]”, GenBank Accession No. WP_019977713.1, Jun. 30, 2013, 1 page. |
| National Center for Biotechnology Information “hypothetical protein [Verrucomicrobia bacterium SCGC AAA300-N18]”, GenBank Accession No. WP_020152244.1, Jun. 30, 2013, 1 page. |
| National Center for Biotechnology Information “fucosyltransferase, partial [Helicobacter pylori]”, GenBank Accession No. WP_020982055.1, Sep. 3, 2013, 1 page. |
| National Center for Biotechnology Information “hypothetical protein [Helicobacter rappini]”, GenBank Accession No. WP_020995419.1, Mar. 2, 2018, 1 page. |
| National Center for Biotechnology Information “Multispecies: Alpha1,2fucosyltransferase [Escherichia]”, GenBank Accession No. WP_021554465.1, Mar. 9, 2018, 1 page. |
| National Center for Biotechnology Information “Alpha1,2fucosyltransferase [Tannerella Sp. CAG:118]”, GenBank Accession No. WP_021929367.1, Dec. 9, 2016, 1 page. |
| National Center for Biotechnology Information “hypothetical protein [Tannerella sp. CAG:118]”, GenBank Accession No. WP_021930564.1, Mar. 25, 2015, 1 page. |
| National Center for Biotechnology Information “Alpha1,2fucosyltransferase [Bacteroides Sp. Cag:633]”, GenBank Accession No. WP_022161880.1, Dec. 9, 2016, 1 page. |
| National Center for Biotechnology Information “hypothetical protein [Akkermansia sp. CAG:344]”, GenBank Accession No. WP_022396409.1, Jan. 22, 2015, 1 page. |
| National Center for Biotechnology Information “hypothetical protein [Prevotella sp. CAG:873]”, GenBank Accession No. WP_022453039.1, Mar. 25, 2015, 1 page. |
| National Center for Biotechnology Information “Alpha1,2fucosyltransferase [Prevotella Sp. Cag:891]”, GenBank Accession No. WP_022481266.1, Dec. 9, 2016, 1 page. |
| National Center for Biotechnology Information “glycosyl transferase [Butyrivibrio fibrisolvens]”, GenBank Accession No. WP_022753767.1, Dec. 8, 2015, 1 page. |
| National Center for Biotechnology Information “glycosyl transferase [Butyrivibrio sp. NC2007]”, GenBank Accession No. WP_022768256.1, Dec. 8, 2015, 1 page. |
| National Center for Biotechnology Information “Alpha1,2fucosyltransferase [Butyrivibrio Sp. Ae2015]”, GenBank Accession No. WP_022772718.1, Oct. 22, 2013, 1 page. |
| National Center for Biotechnology Information “glycosyl transferase [Butyrivibrio sp. AE2015]”, GenBank Accession No. WP_022772782.1, Dec. 8, 2015, 1 page. |
| National Center for Biotechnology Information “hypothetical protein [Butyrivibrio sp. AE3009]”, GenBank Accession No. WP_022777675.1, Oct. 22, 2013, 1 page. |
| National Center for Biotechnology Information “hypothetical protein [Lachnospiraceae bacterium NK4A136]”, GenBank Accession No. WP_022781173.1, Oct. 22, 2013, 1 page. |
| National Center for Biotechnology Information “glycosyl transferase [Lachnospiraceae bacterium NK4A136]”, GenBank Accession No. WP_022781636.1, Dec. 8, 2015, 1 page. |
| National Center for Biotechnology Information “glycosyl transferase [Lachnospiraceae bacterium NK4A179]”, GenBank Accession No. WP_022783468.1, Dec. 8, 2015, 1 page. |
| National Center for Biotechnology Information “Alpha1,2fucosyltransferase [Salmonella enterica]”, GenBank Accession No. WP_023214330.1, Oct. 30, 2013, 1 page. |
| National Center for Biotechnology Information “putative LPS biosynthesis related glycosyltransferase [Bacteroides fragilis NCTC 9343]”, GenBank Accession No. YP_213065.1, Dec. 17, 2014, 2 pages. |
| National Center for Biotechnology Information “putative fucosyltransferase [Bacteroides fragilis NCTC 9343]”, GenBank Accession No. YP_213404.1, Dec. 17, 2014, 2 pages. |
| National Center for Biotechnology Information “LPS biosynthesis related glycosyltransferase [Anaeromyxobacter dehalogenans 2CP-C]”, GenBank Accession No. YP_466262.1, Dec. 17, 2014, 2 pages. |
| National Center for Biotechnology Information “Alpha-(1,3)-fucosyltransferase [Rickettsia bellii RML369-C]”, GenBank Accession No. YP_537673.1, Dec. 17, 2014, 1 page. |
| National Center for Biotechnology Information “Glycosyl Transferase Family Protein [Bacteroides Vulgatus ATCC 8482]”, GenBank Accession No. YP_001300461.1, Jan. 26, 2012, 1 page. |
| National Center for Biotechnology Information “hypothetical protein Amuc_0760 [Akkermansia muciniphila ATCC BAA-835]”, GenBank Accession No. YP_001877377.1, Dec. 17, 2014, 2 pages. |
| National Center for Biotechnology Information “Lipopolysaccharide Biosynthesis Protein [Helicobacter Pylori P12]”, GenBank Accession No. YP_002301261.1, Dec. 17, 2014, 1 page. |
| National Center for Biotechnology Information “Glycosyl Transferase Family Protein [Methanosphaerula Palustris E19c]”, GenBank Accession No. YP_002467213.1, Dec. 16, 2014, 1 page. |
| National Center for Biotechnology Information “LPS biosynthesis glycosyltransferase [Anaeromyxobacter dehalogenans 2CP-1]”, GenBank Accession No. YP_002493655.1, Dec. 17, 2014, 2 pages. |
| National Center for Biotechnology Information “glycosyl transferase [Methanobrevibacter ruminantium M1]”, GenBank Accession No. YP_003424269.1, Dec. 17, 2014, 1 page. |
| National Center for Biotechnology Information “alpha-(1,3)-fucosyltransferase [Azospirillum sp. B510]”, GenBank Accession No. YP_003447891.1, Dec. 17, 2014, 2 pages. |
| National Center for Biotechnology Information “Alpha1,2fucosyltransferase [Helicobacter Mustelae 12198]”, GenBank Accession No. YP_003517185.1, Dec. 17, 2014, 1 page. |
| National Center for Biotechnology Information “alpha-1,3-fucosyltransferase [Coraliomargarita akajimensis DSM 45221]”, GenBank Accession No. YP_003549734.1, Dec. 17, 2014, 2 pages. |
| National Center for Biotechnology Information “Glycosyltransferase Family 11 [Prevotella Melaninogenica ATCC 25845]”, GenBank Accession No. YP_003814512.1, Dec. 17, 2014, 1 page. |
| National Center for Biotechnology Information “alpha (1,3)-fucosyltransferase [Helicobacter bizzozeronii CIII-1]”, GenBank Accession No. YP_004607881.1, Dec. 17, 2014, 1 page. |
| National Center for Biotechnology Information “putative glycosyltransferase (plasmid) [Azospirillum brasilense Sp245]”, GenBank Accession No. YP_004985483.1, Dec. 17, 2014, 2 pages. |
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| Number | Date | Country | |
|---|---|---|---|
| 20170306373 A1 | Oct 2017 | US |
| Number | Date | Country | |
|---|---|---|---|
| 62047851 | Sep 2014 | US |
