ALPHA-AMYLASE VARIANTS AND POLYNUCLEOTIDES ENCODING SAME

Description

REFERENCE TO A SEQUENCE LISTING

This application contains a Sequence Listing in computer readable form, which is incorporated herein by reference.

BACKGROUND OF THE INVENTION
Field of the Invention

The present invention relates to alpha-amylase variants, polynucleotides encoding the variants, methods of producing the variants, and methods of using the variants.

Description of the Related Art

Alpha-amylases have for many years been used in laundry where is it well-known that alpha-amylases have a beneficial effect in removal of starch containing, or starch-based, stains.

WO95/26397 discloses alkaline Bacillus amylases having good wash performance measured at temperatures in the range of 30-60° C.

WO00/60060 and WO00/60058 discloses further bacterial alpha-amylases having good wash performance.

In recent years there has been a desire to reduce the temperature of the laundry in order to reduce the energy consumption. Lowering the temperature in laundry often means that the performance of the detergent composition and the enzyme is reduced and a lower wash performance is therefore obtained at low temperature. It is therefore desired to find new alpha-amylases having good wash performance at low temperature. Accordingly, it is an object of the present invention to provide alpha-amylases which have good wash performance at low temperature, such as at 15° C. Thus, the present invention provides such further improved alpha-amylse variants with improved properties compared to its parent.

SUMMARY OF THE INVENTION

The present invention relates to an alpha-amylase variant of a parent polypeptide having alpha-amylase activity, wherein the variant is a fusion polypeptide which has an improved wash performance at low temperature, and wherein said variant has alpha-amylase activity.

The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of producing the variants.

The present invention also relates to methods of improving wash performance of a parent alpha-amylase.

Conventions for Designation of Variants

For purposes of the present invention, the mature polypeptide disclosed in SEQ ID NO: 7 is used to determine the corresponding amino acid residue in another alpha-amylase. Alternatively, the mature polypeptide disclosed in SEQ ID NO: 13 and 14 may be used to determine the corresponding amino acid residue in another alpha-amylase. The amino acid sequence of another alpha-amylase is aligned with the mature polypeptide disclosed in SEQ ID NO: 7, and based on the alignment, the amino acid position number corresponding to any amino acid residue in the mature polypeptide disclosed in any of SEQ ID NOs: 13 and 14 is determined using the Needleman-Wunsch algorithm (Needleman and Wunsch, 1970, J. Mol. Biol. 48: 443-453) as implemented in the Needle program of the EMBOSS package (EMBOSS:

The European Molecular Biology Open Software Suite, Rice et al., 2000, Trends Genet. 16: 276-277), preferably version 5.0.0 or later. The parameters used are gap open penalty of 10, gap extension penalty of 0.5, and the EBLOSUM62 (EMBOSS version of BLOSUM62) substitution matrix.

Identification of the corresponding amino acid residue in another alpha-amylase can be determined by an alignment of multiple polypeptide sequences using several computer programs including, but not limited to, MUSCLE (multiple sequence comparison by log-expectation; version 3.5 or later; Edgar, 2004, Nucleic Acids Research 32: 1792-1797), MAFFT (version 6.857 or later; Katoh and Kuma, 2002, Nucleic Acids Research 30: 3059-3066; Katoh et al., 2005, Nucleic Acids Research 33: 511-518; Katoh and Toh, 2007, Bioinformatics 23: 372-374; Katoh et al., 2009, Methods in Molecular Biology 537:_39-64; Katoh and Toh, 2010, Bioinformatics 26:_1899-1900), and EMBOSS EMMA employing ClustalW (1.83 or later; Thompson et al., 1994, Nucleic Acids Research 22: 4673-4680), using their respective default parameters.

When the other enzyme has diverged from the mature polypeptide of SEQ ID NO: 7 such that traditional sequence-based comparison fails to detect their relationship (Lindahl and Elofsson, 2000, J. Mol. Biol. 295: 613-615), other pairwise sequence comparison algorithms can be used. Greater sensitivity in sequence-based searching can be attained using search programs that utilize probabilistic representations of polypeptide families (profiles) to search databases. For example, the PSI-BLAST program generates profiles through an iterative database search process and is capable of detecting remote homologs (Atschul et al., 1997, Nucleic Acids Res. 25: 3389-3402). Even greater sensitivity can be achieved if the family or superfamily for the polypeptide has one or more representatives in the protein structure databases. Programs such as GenTHREADER (Jones, 1999, J. Mol. Biol. 287: 797-815; McGuffin and Jones, 2003, Bioinformatics 19: 874-881) utilize information from a variety of sources (PSI-BLAST, secondary structure prediction, structural alignment profiles, and solvation potentials) as input to a neural network that predicts the structural fold for a query sequence. Similarly, the method of Gough et al., 2000, J. Mol. Biol. 313: 903-919, can be used to align a sequence of unknown structure with the superfamily models present in the SCOP database. These alignments can in turn be used to generate homology models for the polypeptide, and such models can be assessed for accuracy using a variety of tools developed for that purpose.

For proteins of known structure, several tools and resources are available for retrieving and generating structural alignments. For example the SCOP superfamilies of proteins have been structurally aligned, and those alignments are accessible and downloadable. Two or more protein structures can be aligned using a variety of algorithms such as the distance alignment matrix (Holm and Sander, 1998, Proteins 33: 88-96) or combinatorial extension (Shindyalov and Bourne, 1998, Protein Engineering 11: 739-747), and implementation of these algorithms can additionally be utilized to query structure databases with a structure of interest in order to discover possible structural homologs (e.g., Holm and Park, 2000, Bioinformatics 16: 566-567).

In describing the variants of the present invention, the nomenclature described below is adapted for ease of reference. The accepted IUPAC single letter or three letter amino acid abbreviation is employed.

Substitutions: For an amino acid substitution, the following nomenclature is used: Original amino acid, position, substituted amino acid. Accordingly, the substitution of threonine at position 226 with alanine is designated as “Thr226Ala” or “T226A”. Multiple mutations are separated by addition marks (“+”), e.g., “Gly205Arg+Ser411Phe” or “G205R+S411F”, representing substitutions at positions 205 and 411 of glycine (G) with arginine (R) and serine

(S) with phenylalanine (F), respectively.

Deletions: For an amino acid deletion, the following nomenclature is used: Original amino acid, position, *. Accordingly, the deletion of glycine at position 195 is designated as “Gly195*” or “G195*”. Multiple deletions are separated by addition marks (“+”), e.g., “Gly195*+Ser411*” or “G195*+S411*”.

Multiple modifications: Variants comprising multiple modifications are separated by addition marks (“+”), e.g., “Arg170Tyr+Gly195Glu” or “R170Y+G195E” representing a substitution of arginine and glycine at positions 170 and 195 with tyrosine and glutamic acid, respectively.

Different modifications: Where different modifications can be introduced at a position, the different alterations are separated by a comma, e.g., “Arg170Tyr,Glu” represents a substitution of arginine at position 170 with tyrosine or glutamic acid. Thus, “Tyr167Gly,Ala+Arg170Gly,Ala” designates the following variants:

“Tyr167Gly+Arg170Gly”, “Tyr167Gly+Arg170Ala”, “Tyr167Ala+Arg170Gly”, and “Tyr167Ala+Arg170Ala”.

DETAILED DESCRIPTION OF THE INVENTION

The present invention relates to a set of sequences. For ease, these are listed below;

SEQ ID NO: 1 is the nucleotide sequence of an alpha-amylase (AAI10)

SEQ ID NO: 2 is the nucleotide sequence of an alpha-amylase originating from Alicyclobacillus sp.

SEQ ID NO: 3 is the nucleotide sequence of an alpha-amylase originating from Bacillus amyloliquefaciens.

SEQ ID NO: 4 is the amino acid sequence of AAI10 including the signal peptide.

SEQ ID NO: 5 is the amino acid sequence of Alicyclobacillus sp. including the signal peptide.

SEQ ID NO: 6 is the amino acid sequence of Bacillus amyloliquefaciens including the signal peptide.

SEQ ID NO: 7 is the amino acid sequence of the mature AAI10

SEQ ID NO: 8 is the amino acid sequence of the mature Alicyclobacillus sp.

SEQ ID NO: 9 is the amino acid sequence of the mature Bacillus amyloliquefaciens

SEQ ID NO: 10 is the amino acid sequence corresponding the A and B domains of AAI10.

SEQ ID NO: 11 is the amino acid sequence corresponding to the C domain of Alicyclobacillus sp.

SEQ ID NO: 12 is the amino acid sequence corresponding to the C domain of Bacillus amyloliquefaciens

SEQ ID NO: 13 is the amino acid sequence of the fusion polypeptide consisting of the A and B domain of AAI10 and the C domain of Alicyclobacillus sp.

SEQ ID NO: 14 is the amino acid sequence of the fusion polypeptide consisting of the A and the B domains of AAI10 and the C domain of Bacillus amyloliquefaciens

SEQ ID NO: 15 is the amino acid sequence of the mature AAI10 alpha-amylase comprising a double deletion at positions corresponding to positions 182 and 183 of SEQ ID NO. 7

Variants of the Invention

In one aspect, the present invention relates to variants of a parent polypeptide having alpha-amylase activity. Thus, in particular aspect, the present invention relates to variant of a parent polypeptide having alpha-amylase activity, wherein said variant is a fusion polypeptide which has an improved wash performance at low temperature, and wherein said variant has alpha-amylase activity.

It has been shown that variants according to the present invention have improved performance, such as wash performance, at a low temperature, in particular at 15° C., in liquid detergent compositions.

The term “variant” as used herein, refers to a polypeptide having alpha-amylase activity comprising an alteration, i.e., a substitution, insertion, and/or deletion, at one or more (e.g., several) positions. A substitution means replacement of the amino acid occupying a position with a different amino acid; a deletion means removal of the amino acid occupying a position; and an insertion means adding an amino acid adjacent to and immediately following the amino acid occupying a position, all as defined above. The variants of the present invention have at least 20%, e.g., at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, or at least 100% of the alpha-amylase activity of the polypeptide of SEQ ID NOs: 7, 13, 14, 15, or the mature polypeptide of SEQ ID NO: 4. The variants of the present invention have at least 65%, such as at least 70%, such as at least 75%, such as at least 80%, such as at least 85%, such at least 90%, such as at least 95%, such as at least 97%, such as at least 99%, but less than 100% sequence identity to any one of SEQ ID NOs: 4, 7, 13, 14, or 15

The term “parent polypeptide” as used herein, refers to a polypeptide to which an alteration is made to produce enzyme variants. The polypeptide having any of SEQ ID NOs: 4, 7, 13, 14, and 15 may e.g. be a parent for the variants of the present invention. Any polypeptide having at least 65%, such as at least 70%, such as at least 75%, such as at least 80%, such as at least 85%, such at least 90%, such as at least 95%, such as at least 97%, such as at least 99%, sequence identity to any one of SEQ ID NOs: 4, 7, 13, 14, or 15 may also be a parent polypeptide for the variants of the present invention.

The parent polypeptide may be a fusion polypeptide or cleavable fusion polypeptide. Such fusion polypeptide may consist of a subsquence of one parent polypeptide and a subsequence of second parent polypeptide. In particular, a fusion polypeptide may consist of an A and B domain of one species of alpha-amylase and a C domain of another species of alpha-amylase, and thereby providing a parent polypeptide to generate a variant of the present invention. Techniques for producing fusion polypeptides are known in the art, and include ligating the coding sequences encoding the polypeptides so that they are in frame and that expression of the fusion polypeptide is under control of the same promoter(s) and terminator. Fusion polypeptides may also be constructed using intein technology in which fusion polypeptides are created post-translationally (Cooper et al., 1993, EMBO J. 12: 2575-2583; Dawson et al., 1994, Science 266: 776-779).

A fusion polypeptide may further comprise a cleavage site between the two polypeptides. Upon secretion of the fusion protein, the site is cleaved releasing the two polypeptides. Examples of cleavage sites include, but are not limited to, the sites disclosed in Martin et al., 2003, J. Ind. Microbiol. Biotechnol. 3: 568-576; Svetina et al., 2000, J. Biotechnol. 76: 245-251; Rasmussen-Wilson et al., 1997, Appl. Environ. Microbiol. 63: 3488-3493; Ward et al., 1995, Biotechnology 13: 498-503; and Contreras et al., 1991, Biotechnology 9: 378-381; Eaton et al., 1986, Biochemistry 25: 505-512; Collins-Racie et al., 1995, Biotechnology 13: 982-987; Carter et al., 1989, Proteins: Structure, Function, and Genetics 6: 240-248; and Stevens, 2003, Drug Discovery World 4: 35-48.

The parent polypeptide may be obtained from microorganisms of any genus. For purposes of the present invention, the term “obtained from” as used herein in connection with a given source shall mean that the parent polypeptide encoded by a polynucleotide is produced by the source or by a strain in which the polynucleotide from the source has been inserted.

In some aspects of the present invention, the parent polypeptide is Bacillus sp. alpha-amylase, e.g., the alpha-amylase of SEQ ID NOs: 4, the mature polypeptide thereof, i.e. SEQ ID NO: 7. In particular aspects, the parent polypeptide is a fusion protein, and originates from both a Bacillus sp and a Alicyclobacillus sp., e.g. the alpha-amylase of SEQ ID NO: 13. In other aspects, the parent polypeptide is a fusion protein and originates from two different Bacillus sps., e.g. resulting in the alpha-amylase of SEQ ID NO: 14.

It will be understood that for the aforementioned species, the invention encompasses both the perfect and imperfect states, and other taxonomic equivalents, e.g., anamorphs, regardless of the species name by which they are known. Those skilled in the art will readily recognize the identity of appropriate equivalents.

Strains of these species are readily accessible to the public in a number of culture collections, such as the American Type Culture Collection (ATCC), Deutsche Sammlung von Mikroorganismen and Zellkulturen GmbH (DSMZ), Centraalbureau Voor Schimmelcultures (CBS), and Agricultural Research Service Patent Culture Collection, Northern Regional Research Center (NRRL).

The parent polypeptide may be identified and obtained from other sources including microorganisms isolated from nature (e.g., soil, composts, water, etc.) or DNA samples obtained directly from natural materials (e.g., soil, composts, water, etc.) using the above-mentioned probes. Techniques for isolating microorganisms and DNA directly from natural habitats are well known in the art. A polynucleotide encoding a parent polypeptide may then be obtained by similarly screening a genomic DNA or cDNA library of another microorganism or mixed DNA sample. Once a polynucleotide encoding a parent polypeptide has been detected with the probe(s), the polynucleotide can be isolated or cloned by utilizing techniques that are known to those of ordinary skill in the art (see, e.g., Sambrook et al., 1989, supra).

In one embodiment, the parent polypeptide comprises or consists of the amino acid sequence set forth in SEQ ID NO: 7, 13, or 15. In another embodiment, the parent polypeptide comprises or consists of the mature polypeptide of SEQ ID NO: 4. In particular, the parent polypeptide may comprise or consist of amino acids 1 to 485 of SEQ ID NO: 4.

In another embodiment, the parent polypeptide is a fragment of the mature polypeptide of SEQ ID NO: 4 containing at least 350, such as at least 390 amino acid residues, e.g., at least 395 and at least 397 amino acid residues of SEQ ID NO: 4.

In another embodiment, the parent polypeptide is an allelic variant of the mature polypeptide of SEQ ID NO: 4.

The term “sequence identity” as used herein, refers to the relatedness between two amino acid sequences or between two nucleotide sequences. For purposes of the present invention, the sequence identity between two amino acid sequences is determined using the Needleman-Wunsch algorithm (Needleman and Wunsch, 1970, J. Mol. Biol. 48: 443-453) as implemented in the Needle program of the EMBOSS package (EMBOSS: The European Molecular Biology Open Software Suite, Rice et al., 2000, Trends Genet. 16: 276-277), preferably version 5.0.0 or later. The parameters used are gap open penalty of 10, gap extension penalty of 0.5, and the EBLOSUM62 (EMBOSS version of BLOSUM62) substitution matrix. The output of Needle labeled “longest identity” (obtained using the -nobrief option) is used as the percent identity and is calculated as follows:

(Identical Residues×100)/(Length of Alignment−Total Number of Gaps in Alignment)

For purposes of the present invention, the sequence identity between two deoxyribonucleotide sequences is determined using the Needleman-Wunsch algorithm (Needleman and Wunsch, 1970, supra) as implemented in the Needle program of the EMBOSS package (EMBOSS: The European Molecular Biology Open Software Suite, Rice et al., 2000, supra), preferably version 5.0.0 or later. The parameters used are gap open penalty of 10, gap extension penalty of 0.5, and the EDNAFULL (EMBOSS version of NCBI NUC4.4) substitution matrix. The output of Needle labeled “longest identity” (obtained using the -nobrief option) is used as the percent identity and is calculated as follows:

(Identical Deoxyribonucleotides×100)/(Length of Alignment−Total Number of Gaps in Alignment)

The term “alpha-amylase activity” as used herein, refers to the activity of alpha-1,4-glucan-4-glucanohydrolases, E.C. 3.2.1.1, which constitute a group of enzymes, catalyzing hydrolysis of starch and other linear and branched 1,4-glucosidic oligo- and polysaccharides. Thus, the term “alpha-amylase” as used herein, refers to an enzyme that has alpha-amylase activity (Enzyme Class; EC 3.2.1.1) that hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen, yielding glucose and maltose. The terms “alpha-amylase” and “amylase” may be used interchangeably and constitute the same meaning and purpose within the scope of the present invention. For purposes of the present invention, alpha-amylase activity is determined according to the procedure described in the Examples. In one embodiment, the variants of the present invention have at least 20%, e.g., at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, or at least 100% of the alpha-amylase activity of the polypeptide of SEQ ID NOs: 7, 13, 14, or 15; or the mature polypeptide of SEQ ID NO: 4.

The term “fusion polypeptide” as used herein, refers to a polypeptide which comprises amino acid sequences originating from more than one, such as two, three, or four, species. Such a fusion polypeptide may have been generated by molecular techniques well-known to the skilled person. A fusion polypeptide of the present invention has alpha-amylase activity. In particular, a fusion polypeptide of the present invention, comprises e.g. an A and B domain of one alpha-amylase and a C domain from another alpha-amylase.

The term “wash performance” as used herein, refers to an enzyme's ability to remove starch or starch-containing stains present on the object to be cleaned during e.g. laundry or hard surface cleaning, such as dish wash. The term “wash performance” includes cleaning in general e.g. hard surface cleaning as in dish wash, but also wash performance on textiles such as laundry, and also industrial and institutional cleaning. The wash performance may be quantified by calculating the so-called Intensity value, and results may be displayed as “Improvement Factor” (IF). Wash performance may be determined as in described in the Examples herein.

The term “Intensity value” as used herein, refers to the wash performance measured as the brightness expressed as the intensity of the light reflected from the sample when illuminated with white light. When the sample is stained the intensity of the reflected light is lower, than that of a clean sample. Therefore the intensity of the reflected light can be used to measure wash performance, where a higher intensity value correlates with higher wash performance.

Color measurements are made with a professional flatbed scanner (Kodak iQsmart, Kodak) used to capture an image of the washed textile.

To extract a value for the light intensity from the scanned images, 24-bit pixel values from the image are converted into values for red, green and blue (RGB). The intensity value (Int) is calculated by adding the RGB values together as vectors and then taking the length of the resulting vector:

Int=√{square root over (r²+g²+b²)}

The term “improved wash performance” as used herein, refers to an improvement of the wash performance of an alpha-amylase of the present invention relative to the wash performance of the parent polypeptide. Improved wash performance may be measured by comparing of the so-called Intensity value and calculating the Improvement Factor (IF). The improved wash performance is determined according to the section “Wash performance of alpha-amylases using Automatic Mechanical Stress Assay” and using model detergent J at 15° C., 30° C. or 40° C. Other model detergents may be used, such as Model detergent A.

Thus, in one embodiment, the improved wash performance is determined by a method comprising the steps of;

- a) washing a fabric stained with starch with an alpha-amylase variant and a parent polypeptide sample added, respectively, to a model detergent composition, such as Model A or Model J;
- b) measuring the intensity of light reflected from the sample when illuminated with white light; and
- c) optionally, calculating the improvement factor (IF) as the ratio of delta intensity of the alpha-amylase sample over the delta intensity of the parent polypeptide sample.

In one embodiment, the improved wash performance is determined by a method comprising the steps of;

- a) washing a fabric stained with starch with an alpha-amylase variant and a parent polypeptide sample added, respectively, to a model detergent composition, such as Model A or Model J for 20 minutes at 15° C., 30° C., and 40° C.;
- b) measuring the intensity of light reflected from the sample when illuminated with white light; and
- c) optionally, calculating the improvement factor (IF) as the ration of delta intensity of the alpha-amylase sample over the delta intensity of the parent polypeptide sample.

The term “low temperature” as used herein, refers to is a temperature of 5-40° C., such as 5-35° C., preferably 5-30° C., more preferably 5-25° C., more preferably 5-20° C., most preferably 5-15° C., and in particular 5-10° C. In a preferred embodiment, “Low temperature” is a temperature of 10-35° C., preferably 10-30° C., more preferably 10-25° C., most preferably 10-20° C., and in particular 10-15° C. Most preferred, low temperature means 15° C.

Thus, in one embodiment, the low temperature is 5° C. to 40° C., preferably 10° C. to 30°, event more preferred 15° C. to 20° C.

In one embodiment, the improved wash performance has an Improvement Factor (IF) of ≥1 when compared to said parent polypeptide having alpha-amylase activity.

The term “Improvement Factor” as used herein, refers to a quantitative way of calculating the improvement of a particular property of a variant according to the present invention. Determination of the Improvement Factor may be according to the following formula:

$\frac{Intensity value of variant - Intensity value of blank}{Intensity value of parent - Intensity value of blank}$

Other formulas may be used to determine the Improvement Factor. The skilled person knows the presently presented formula as well as alternative ways of calculating the Improvement Factor.

According to the present invention, a value of 1.0 corresponds to the performance observed for the parent polypeptide. A value above 1.0 indicates an improvement of performance of the variant tested compared to the parent polypeptide. Accordingly, any value of ≥1.0 is indicative for improvement of property, such as performance, of the variant compared to the parent polypeptide.

According to the present invention, a variant showing improvement of property under at least one condition tested, is considered a variant having improved property as compared to the parent polypeptide.

In one embodiment, said parent polypeptide comprises an A and a B domain comprising the amino acid sequence of SEQ ID NO: 10, or an A and a B domain having at least 80%, such as 85%, such as 90%, such as 95%, such as 97% sequence identity to SEQ ID NO: 10, and a C domain of a low temperature alpha-amylase.

The terms “A domain”, “B domain” and “C domain” as used herein, refers to three distinct domains A, B and C, all part of the alpha-amylase structure, see, e.g., Machius et al., 1995, J. Mol. Biol. 246: 545-559. Thus, an alpha-amylase, such as a parent polypeptide and a variant according to the invention, may comprise both an A, B, and C domain. The term “domain” means a region of a polypeptide that in itself forms a distinct and independent substructure of the whole molecule. Alpha-amylases consist of a beta/alpha-8 barrel harboring the active site residues, which is denoted the A domain, a rather long loop between the beta-sheet 3 and alpha-helix 3, which is denoted the B domain (together; “A and B domain”), and a C-domain and in some cases also a carbohydrate binding domain (e.g., WO 2005/001064; Machius et al., supra).

The domains of an alpha-amylase may be determined by structure analysis such as using crystallographically techniques. An alternative method for determining the domains of an alpha-amylase is by sequence alignment of the amino acid sequence of the alpha-amylase with another alpha-amylase for which the domains have been determined. The sequence that aligns with, e.g., the C-domain sequence in the alpha-amylase for which the C-domain has been determined can be considered the C domain for the given alpha-amylase.

The term “A and B domain” as used herein, refers to two domains of an alpha-amylase taken as one unit, whereas the C domain is another unit of the alpha-amylases. Thus, the amimo acid sequence of the “A and B domain” is understood as one sequence or one part of a sequence of an alpha-amylase comprising an “A and B domain” and other domains (such as the C domain). Thus, the term “the A and B domain domain has at least 65% sequence identity to SEQ ID NO: 10” means that the amino acid sequence that form the A and B domain has at least 65% sequence identity to SEQ ID NO: 10. As used herein, the “A and B domain” of a parent polypeptide corresponds to amino acids 1 to 399 of SEQ ID NO: 7, or to amino acids 1 to 397 of SEQ ID NO: 15. The A and B domain of a parent polypeptide may have the amino acid sequence of SEQ ID NO: 10.

The term “C domain” as used herein, refers to a domain of an alpha-amylase as one unit. The “C domain” of an alpha-amylase corresponds to amino acids 400 to 485 of SEQ ID NO: 7, or to amino acid 398 to 483 of SEQ ID NO: 15. Thus, the C domain of an alpha amylase may be found by alignment of said alpha-amylase with the polypeptide of SEQ ID NO: 7 or 15. The part of said alpha-amylase that aligns with amino acids 400 to 485 of SEQ ID NO: 7 or to amino acids 398 to 483 of SEQ ID NO: 15 is according to the present invention “the C domain” of the alpha-amylase. The C domain of a parent polypeptide may have the amino acid sequence of SEQ ID NOs: 11 or 12.

The term “C domain of a low temperature alpha-amylase” as used herein, refers to the C domain of an alpha-amylase showing particular properties at low temperatures. Non-limiting examples of such low temperature alpha-amylases are those originating from Alicyclobacillus sp. as well as from Bacillus amyloliquefaciens (BAN).

In one embodiment, the parent polypeptide comprises a C domain comprising the amino acid sequence of SEQ ID NOs: 11 or 12, or a C domain having at 80%, such as 85%, such as 90%, such as 95%, such as 97%, sequence identity to SEQ ID NO: 11 or 12.

In one embodiment, the parent polypeptide comprises the amino acid sequence of SEQ ID NOs: 13 or 14, or a sequence having at least 80%, such as 85%, such as 90%, such as 95%, such as 97% sequence identity to SEQ ID NOs: 13 or 14.

As stated elsewhere herein, the parent polypeptide may be any polypeptide having alpha-amylase activity and at least 65% sequence identity to any one of the amino acid sequences as set forth in SEQ ID NOs: 13 and 14. In certain embodiments, the parent polypeptide comprises the amino acid sequences as set forth in SEQ ID NOs: 10, 11, and 12.

In one embodiment, the parent polypeptide a sequence identity to the amino acid sequence set forth in SEQ ID NOs: 13 or 14 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%, and which parent polypeptide has alpha-amylase activity. In one embodiment, the amino acid sequence of the parent polypeptide differs by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the amino acid sequences set forth in SEQ ID NOs: 7, 13, and 14.

The present invention provides variants of such parent polypeptides.

The variants according to the present invention may advantageously comprise alterations in specific positions of the A and B domain. Thus, in a particular embodiment, the variant comprises amino acid alterations in amino acid positions corresponding the amino acid sequence set forth in SEQ ID NO: 10, or a sequence having at least 65% sequence identity to the amino acid sequence set forth in SEQ ID NO: 10.

In particular, the present invention relates to an embodiment, the A and B domains comprise a modification in one or more of the following positions corresponding to positions; H1, T5, G7, Q11, N16, V17, Q32, A37, T40, P45, W48, G50, T51, N54, V56, A60, K72, R87, Q98, M105, G109, F113, R116, Q118, Q125, G133, T134, W140, G142, G149, T165, W167, Q169, R171, Q172, L173, A174, G184, A186, E190, T193, N195, G196, A204, V206, P211, I214, V215, L217, L219, A225, L228, L235, V238, K242, S244, M246, M248, L250, G255, Q256, N260, A263, V264, A265, Y267, K269, N270, G273, S280, W284, T285, M286, F289, V291, N295, Q299, S304, R320, H321, S323, H324, V326, F328, T334, D337, Q345, G346, G348, T355, S376, D377, D379, Y382, S383, Q385, K391, K393, and Q395 of SEQ ID NOs: 7 or 10.

Such variants have shown to have improved wash performance in at least one assay wherein the temperature under which the variants have been tested was a low temperature, such as 15° C., 30° and 40° C. Specific conditions may be seen in the Examples herein.

The term “modification” as used herein, refers to both substitutions and deletions of amino acid within the amino acid sequence of a polypeptide. The terms “alteration” and “modification” may be used interchangeably herein. This should not be understood as any limitation and thus, the terms constitute the same meaning and purpose unless explicitly stated otherwise.

The term “corresponding to” as used herein, refers to way of determining the specific amino acid of a sequence wherein reference is made to a specific amino acid sequence. E.g. for the purposes of the present invention, when references are made to specific amino acid positions, the skilled person would be able to align another amino acid sequence to said amino acid sequence that reference has been made to, in order to determine which specific amino acid may be of interest in said another amino acid sequence. Alignment of another amino acid sequence with e.g. the sequence as set forth in SEQ ID NO: 4, 7, or any other sequence listed herein, has been described elsewhere herein. Alternative alignment methods may be used, and are well-known for the skilled person.

In a particular embodiment, the variant comprises an alteration in two, three, four, five, six, seven, eight, nine, ten, eleven, twelf, thirteen, fourteen, fifiteen, or sixteen positions corresponding to the following positions; H1, T5, G7, Q11, N16, V17, Q32, A37, T40, P45, W48, G50, T51, N54, V56, A60, K72, R87, Q98, M105, G109, F113, R116, Q118, Q125, G133, T134, W140, G142, G149, T165, W167, Q169, R171, Q172, L173, A174, G184, A186, E190, T193, N195, G196, A204, V206, P211, I214, V215, L217, L219, A225, L228, L235, V238, K242, S244, M246, M248, L250, G255, Q256, N260, A263, V264, A265, Y267, K269, N270, G273, S280, W284, T285, M286, F289, V291, Y295, Q299, S304, R320, H321, S323, H324, V326, F328, T334, D337, Q345, G346, G348, T355, S376, D377, D379, Y382, S383, Q385, K391, K393, and Q395 of SEQ ID NOs: 7 or 10.

In a particular embodiment, the variant comprises an alteration in the following positions;

H1 + T5
T40 + L217
N54 + T285
Q125 + G255
G184 + P211
M248 + Q299

H1 + G7
T40 + L219
N54 + M286
Q125 + Q256
G184 + I214
M248 + S304

H1 + Q11
T40 + L228
N54 + F289
Q125 + A263
G184 + V215
M248 + R320

H1 + N16
T40 + L235
N54 + V291
Q125 + V264
G184 + L217
M248 + H321

H1 + V17
T40 + V238
N54 + Q299
Q125 + A265
G184 + L219
M248 + S323

H1 + Q32
T40 + M246
N54 + S304
Q125 + Y267
G184 + L228
M248 + H324

H1 + A37
T40 + M248
N54 + R320
Q125 + N270
G184 + L235
M248 + F328

H1 + T40
T40 + L250
N54 + H321
Q125 + G273
G184 + V238
M248 + T334

H1 + P45
T40 + G255
N54 + S323
Q125 + S280
G184 + M246
M248 + D337

H1 + W48
T40 + Q256
N54 + H324
Q125 + T285
G184 + M248
M248 + Q345

H1 + G50
T40 + A263
N54 + F328
Q125 + M286
G184 + L250
M248 + G346

H1 + T51
T40 + V264
N54 + T334
Q125 + F289
G184 + G255
M248 + G348

H1 + N54
T40 + A265
N54 + D337
Q125 + V291
G184 + Q256
M248 + T355

H1 + V56
T40 + Y267
N54 + Q345
Q125 + Q299
G184 + A263
M248 + S376

H1 + A60
T40 + N270
N54 + G346
Q125 + S304
G184 + V264
M248 + D377

H1 + K72
T40 + G273
N54 + G348
Q125 + R320
G184 + A265
M248 + D379

H1 + R87
T40 + S280
N54 + T355
Q125 + H321
G184 + Y267
M248 + Y382

H1 + Q98
T40 + T285
N54 + S376
Q125 + S323
G184 + N270
M248 + S383

H1 + M105
T40 + M286
N54 + D377
Q125 + H324
G184 + G273
M248 + Q385

H1 + G109
T40 + F289
N54 + D379
Q125 + F328
G184 + S280
M248 + K391

H1 + F113
T40 + V291
N54 + Y382
Q125 + T334
G184 + T285
M248 + K393

H1 + R116
T40 + Q299
N54 + S383
Q125 + D337
G184 + M286
M248 + Q395

H1 + Q118
T40 + S304
N54 + Q385
Q125 + Q345
G184 + F289
L250 + G255

H1 + Q125
T40 + R320
N54 + K391
Q125 + G346
G184 + V291
L250 + Q256

H1 + G133
T40 + H321
N54 + K393
Q125 + G348
G184 + Q299
L250 + A263

H1 + T134
T40 + S323
N54 + Q395
Q125 + T355
G184 + S304
L250 + V264

H1 + W140
T40 + H324
V56 + A60
Q125 + S376
G184 + R320
L250 + A265

H1 + G142
T40 + F328
V56 + K72
Q125 + D377
G184 + H321
L250 + Y267

H1 + G149
T40 + T334
V56 + R87
Q125 + D379
G184 + S323
L250 + N270

H1 + T165
T40 + D337
V56 + Q98
Q125 + Y382
G184 + H324
L250 + G273

H1 + W167
T40 + Q345
V56 + M105
Q125 + S383
G184 + F328
L250 + S280

H1 + R171
T40 + G346
V56 + G109
Q125 + Q385
G184 + T334
L250 + T285

H1 + Q172
T40 + G348
V56 + F113
Q125 + K391
G184 + D337
L250 + M286

H1 + L173
T40 + T355
V56 + R116
Q125 + K393
G184 + Q345
L250 + F289

H1 + A174
T40 + S376
V56 + Q118
Q125 + Q395
G184 + G346
L250 + V291

H1 + G184
T40 + D377
V56 + Q125
G133 + T134
G184 + G348
L250 + Q299

H1 + T193
T40 + D379
V56 + G133
G133 + W140
G184 + T355
L250 + S304

H1 + N195
T40 + Y382
V56 + T134
G133 + G142
G184 + S376
L250 + R320

H1 + G196
T40 + S383
V56 + W140
G133 + G149
G184 + D377
L250 + H321

H1 + A204
T40 + Q385
V56 + G142
G133 + T165
G184 + D379
L250 + S323

H1 + V206
T40 + K391
V56 + G149
G133 + W167
G184 + Y382
L250 + H324

H1 + P211
T40 + K393
V56 + T165
G133 + R171
G184 + S383
L250 + F328

H1 + I214
T40 + Q395
V56 + W167
G133 + Q172
G184 + Q385
L250 + T334

H1 + V215
P45 + W48
V56 + R171
G133 + L173
G184 + K391
L250 + D337

H1 + L217
P45 + G50
V56 + Q172
G133 + A174
G184 + K393
L250 + Q345

H1 + L219
P45 + T51
V56 + L173
G133 + G184
G184 + Q395
L250 + G346

H1 + L228
P45 + N54
V56 + A174
G133 + T193
T193 + N195
L250 + G348

H1 + L235
P45 + V56
V56 + G184
G133 + N195
T193 + G196
L250 + T355

H1 + V238
P45 + A60
V56 + T193
G133 + G196
T193 + A204
L250 + S376

H1 + M246
P45 + K72
V56 + N195
G133 + A204
T193 + V206
L250 + D377

H1 + M248
P45 + R87
V56 + G196
G133 + V206
T193 + P211
L250 + D379

H1 + L250
P45 + Q98
V56 + A204
G133 + P211
T193 + I214
L250 + Y382

H1 + G255
P45 + M105
V56 + V206
G133 + I214
T193 + V215
L250 + S383

H1 + Q256
P45 + G109
V56 + P211
G133 + V215
T193 + L217
L250 + Q385

H1 + A263
P45 + F113
V56 + I214
G133 + L217
T193 + L219
L250 + K391

H1 + V264
P45 + R116
V56 + V215
G133 + L219
T193 + L228
L250 + K393

H1 + A265
P45 + Q118
V56 + L217
G133 + L228
T193 + L235
L250 + Q395

H1 + Y267
P45 + Q125
V56 + L219
G133 + L235
T193 + V238
G255 + Q256

H1 + N270
P45 + G133
V56 + L228
G133 + V238
T193 + M246
G255 + A263

H1 + G273
P45 + T134
V56 + L235
G133 + M246
T193 + M248
G255 + V264

H1 + S280
P45 + W140
V56 + V238
G133 + M248
T193 + L250
G255 + A265

H1 + T285
P45 + G142
V56 + M246
G133 + L250
T193 + G255
G255 + Y267

H1 + M286
P45 + G149
V56 + M248
G133 + G255
T193 + Q256
G255 + N270

H1 + F289
P45 + T165
V56 + L250
G133 + Q256
T193 + A263
G255 + G273

H1 + V291
P45 + W167
V56 + G255
G133 + A263
T193 + V264
G255 + S280

H1 + Q299
P45 + R171
V56 + Q256
G133 + V264
T193 + A265
G255 + T285

H1 + S304
P45 + Q172
V56 + A263
G133 + A265
T193 + Y267
G255 + M286

H1 + R320
P45 + L173
V56 + V264
G133 + Y267
T193 + N270
G255 + F289

H1 + H321
P45 + A174
V56 + A265
G133 + N270
T193 + G273
G255 + V291

H1 + S323
P45 + G184
V56 + Y267
G133 + G273
T193 + S280
G255 + Q299

H1 + H324
P45 + T193
V56 + N270
G133 + S280
T193 + T285
G255 + S304

H1 + F328
P45 + N195
V56 + G273
G133 + T285
T193 + M286
G255 + R320

H1 + T334
P45 + G196
V56 + S280
G133 + M286
T193 + F289
G255 + H321

H1 + D337
P45 + A204
V56 + T285
G133 + F289
T193 + V291
G255 + S323

H1 + Q345
P45 + V206
V56 + M286
G133 + V291
T193 + Q299
G255 + H324

H1 + G346
P45 + P211
V56 + F289
G133 + Q299
T193 + S304
G255 + F328

H1 + G348
P45 + I214
V56 + V291
G133 + S304
T193 + R320
G255 + T334

H1 + T355
P45 + V215
V56 + Q299
G133 + R320
T193 + H321
G255 + D337

H1 + S376
P45 + L217
V56 + S304
G133 + H321
T193 + S323
G255 + Q345

H1 + D377
P45 + L219
V56 + R320
G133 + S323
T193 + H324
G255 + G346

H1 + D379
P45 + L228
V56 + H321
G133 + H324
T193 + F328
G255 + G348

H1 + Y382
P45 + L235
V56 + S323
G133 + F328
T193 + T334
G255 + T355

H1 + S383
P45 + V238
V56 + H324
G133 + T334
T193 + D337
G255 + S376

H1 + Q385
P45 + M246
V56 + F328
G133 + D337
T193 + Q345
G255 + D377

H1 + K391
P45 + M248
V56 + T334
G133 + Q345
T193 + G346
G255 + D379

H1 + K393
P45 + L250
V56 + D337
G133 + G346
T193 + G348
G255 + Y382

H1 + Q395
P45 + G255
V56 + Q345
G133 + G348
T193 + T355
G255 + S383

T5 + G7
P45 + Q256
V56 + G346
G133 + T355
T193 + S376
G255 + Q385

T5 + Q11
P45 + A263
V56 + G348
G133 + S376
T193 + D377
G255 + K391

T5 + N16
P45 + V264
V56 + T355
G133 + D377
T193 + D379
G255 + K393

T5 + V17
P45 + A265
V56 + S376
G133 + D379
T193 + Y382
G255 + Q395

T5 + Q32
P45 + Y267
V56 + D377
G133 + Y382
T193 + S383
Q256 + A263

T5 + A37
P45 + N270
V56 + D379
G133 + S383
T193 + Q385
Q256 + V264

T5 + T40
P45 + G273
V56 + Y382
G133 + Q385
T193 + K391
Q256 + A265

T5 + P45
P45 + S280
V56 + S383
G133 + K391
T193 + K393
Q256 + Y267

T5 + W48
P45 + T285
V56 + Q385
G133 + K393
T193 + Q395
Q256 + N270

T5 + G50
P45 + M286
V56 + K391
G133 + Q395
N195 + G196
Q256 + G273

T5 + T51
P45 + F289
V56 + K393
T134 + W140
N195 + A204
Q256 + S280

T5 + N54
P45 + V291
V56 + Q395
T134 + G142
N195 + V206
Q256 + T285

T5 + V56
P45 + Q299
A60 + K72
T134 + G149
N195 + P211
Q256 + M286

T5 + A60
P45 + S304
A60 + R87
T134 + T165
N195 + I214
Q256 + F289

T5 + K72
P45 + R320
A60 + Q98
T134 + W167
N195 + V215
Q256 + V291

T5 + R87
P45 + H321
A60 + M105
T134 + R171
N195 + L217
Q256 + Q299

T5 + Q98
P45 + S323
A60 + G109
T134 + Q172
N195 + L219
Q256 + S304

T5 + M105
P45 + H324
A60 + F113
T134 + L173
N195 + L228
Q256 + R320

T5 + G109
P45 + F328
A60 + R116
T134 + A174
N195 + L235
Q256 + H321

T5 + F113
P45 + T334
A60 + Q118
T134 + G184
N195 + V238
Q256 + S323

T5 + R116
P45 + D337
A60 + Q125
T134 + T193
N195 + M246
Q256 + H324

T5 + Q118
P45 + Q345
A60 + G133
T134 + N195
N195 + M248
Q256 + F328

T5 + Q125
P45 + G346
A60 + T134
T134 + G196
N195 + L250
Q256 + T334

T5 + G133
P45 + G348
A60 + W140
T134 + A204
N195 + G255
Q256 + D337

T5 + T134
P45 + T355
A60 + G142
T134 + V206
N195 + Q256
Q256 + Q345

T5 + W140
P45 + S376
A60 + G149
T134 + P211
N195 + A263
Q256 + G346

T5 + G142
P45 + D377
A60 + T165
T134 + I214
N195 + V264
Q256 + G348

T5 + G149
P45 + D379
A60 + W167
T134 + V215
N195 + A265
Q256 + T355

T5 + T165
P45 + Y382
A60 + R171
T134 + L217
N195 + Y267
Q256 + S376

T5 + W167
P45 + S383
A60 + Q172
T134 + L219
N195 + N270
Q256 + D377

T5 + R171
P45 + Q385
A60 + L173
T134 + L228
N195 + G273
Q256 + D379

T5 + Q172
P45 + K391
A60 + A174
T134 + L235
N195 + S280
Q256 + Y382

T5 + L173
P45 + K393
A60 + G184
T134 + V238
N195 + T285
Q256 + S383

T5 + A174
P45 + Q395
A60 + T193
T134 + M246
N195 + M286
Q256 + Q385

T5 + G184
W48 + G50
A60 + N195
T134 + M248
N195 + F289
Q256 + K391

T5 + T193
W48 + T51
A60 + G196
T134 + L250
N195 + V291
Q256 + K393

T5 + N195
W48 + N54
A60 + A204
T134 + G255
N195 + Q299
Q256 + Q395

T5 + G196
W48 + V56
A60 + V206
T134 + Q256
N195 + S304
A263 + V264

T5 + A204
W48 + A60
A60 + P211
T134 + A263
N195 + R320
A263 + A265

T5 + V206
W48 + K72
A60 + I214
T134 + V264
N195 + H321
A263 + Y267

T5 + P211
W48 + R87
A60 + V215
T134 + A265
N195 + S323
A263 + N270

T5 + I214
W48 + Q98
A60 + L217
T134 + Y267
N195 + H324
A263 + G273

T5 + V215
W48 + M105
A60 + L219
T134 + N270
N195 + F328
A263 + S280

T5 + L217
W48 + G109
A60 + L228
T134 + G273
N195 + T334
A263 + T285

T5 + L219
W48 + F113
A60 + L235
T134 + S280
N195 + D337
A263 + M286

T5 + L228
W48 + R116
A60 + V238
T134 + T285
N195 + Q345
A263 + F289

T5 + L235
W48 + Q118
A60 + M246
T134 + M286
N195 + G346
A263 + V291

T5 + V238
W48 + Q125
A60 + M248
T134 + F289
N195 + G348
A263 + Q299

T5 + M246
W48 + G133
A60 + L250
T134 + V291
N195 + T355
A263 + S304

T5 + M248
W48 + T134
A60 + G255
T134 + Q299
N195 + S376
A263 + R320

T5 + L250
W48 + W140
A60 + Q256
T134 + S304
N195 + D377
A263 + H321

T5 + G255
W48 + G142
A60 + A263
T134 + R320
N195 + D379
A263 + S323

T5 + Q256
W48 + G149
A60 + V264
T134 + H321
N195 + Y382
A263 + H324

T5 + A263
W48 + T165
A60 + A265
T134 + S323
N195 + S383
A263 + F328

T5 + V264
W48 + W167
A60 + Y267
T134 + H324
N195 + Q385
A263 + T334

T5 + A265
W48 + R171
A60 + N270
T134 + F328
N195 + K391
A263 + D337

T5 + Y267
W48 + Q172
A60 + G273
T134 + T334
N195 + K393
A263 + Q345

T5 + N270
W48 + L173
A60 + S280
T134 + D337
N195 + Q395
A263 + G346

T5 + G273
W48 + A174
A60 + T285
T134 + Q345
G196 + A204
A263 + G348

T5 + S280
W48 + G184
A60 + M286
T134 + G346
G196 + V206
A263 + T355

T5 + T285
W48 + T193
A60 + F289
T134 + G348
G196 + P211
A263 + S376

T5 + M286
W48 + N195
A60 + V291
T134 + T355
G196 + I214
A263 + D377

T5 + F289
W48 + G196
A60 + Q299
T134 + S376
G196 + V215
A263 + D379

T5 + V291
W48 + A204
A60 + S304
T134 + D377
G196 + L217
A263 + Y382

T5 + Q299
W48 + V206
A60 + R320
T134 + D379
G196 + L219
A263 + S383

T5 + S304
W48 + P211
A60 + H321
T134 + Y382
G196 + L228
A263 + Q385

T5 + R320
W48 + I214
A60 + S323
T134 + S383
G196 + L235
A263 + K391

T5 + H321
W48 + V215
A60 + H324
T134 + Q385
G196 + V238
A263 + K393

T5 + S323
W48 + L217
A60 + F328
T134 + K391
G196 + M246
A263 + Q395

T5 + H324
W48 + L219
A60 + T334
T134 + K393
G196 + M248
V264 + A265

T5 + F328
W48 + L228
A60 + D337
T134 + Q395
G196 + L250
V264 + Y267

T5 + T334
W48 + L235
A60 + Q345
W140 + G142
G196 + G255
V264 + N270

T5 + D337
W48 + V238
A60 + G346
W140 + G149
G196 + Q256
V264 + G273

T5 + Q345
W48 + M246
A60 + G348
W140 + T165
G196 + A263
V264 + S280

T5 + G346
W48 + M248
A60 + T355
W140 + W167
G196 + V264
V264 + T285

T5 + G348
W48 + L250
A60 + S376
W140 + R171
G196 + A265
V264 + M286

T5 + T355
W48 + G255
A60 + D377
W140 + Q172
G196 + Y267
V264 + F289

T5 + S376
W48 + Q256
A60 + D379
W140 + L173
G196 + N270
V264 + V291

T5 + D377
W48 + A263
A60 + Y382
W140 + A174
G196 + G273
V264 + Q299

T5 + D379
W48 + V264
A60 + S383
W140 + G184
G196 + S280
V264 + S304

T5 + Y382
W48 + A265
A60 + Q385
W140 + T193
G196 + T285
V264 + R320

T5 + S383
W48 + Y267
A60 + K391
W140 + N195
G196 + M286
V264 + H321

T5 + Q385
W48 + N270
A60 + K393
W140 + G196
G196 + F289
V264 + S323

T5 + K391
W48 + G273
A60 + Q395
W140 + A204
G196 + V291
V264 + H324

T5 + K393
W48 + S280
K72 + R87
W140 + V206
G196 + Q299
V264 + F328

T5 + Q395
W48 + T285
K72 + Q98
W140 + P211
G196 + S304
V264 + T334

G7 + Q11
W48 + M286
K72 + M105
W140 + I214
G196 + R320
V264 + D337

G7 + N16
W48 + F289
K72 + G109
W140 + V215
G196 + H321
V264 + Q345

G7 + V17
W48 + V291
K72 + F113
W140 + L217
G196 + S323
V264 + G346

G7 + Q32
W48 + Q299
K72 + R116
W140 + L219
G196 + H324
V264 + G348

G7 + A37
W48 + S304
K72 + Q118
W140 + L228
G196 + F328
V264 + T355

G7 + T40
W48 + R320
K72 + Q125
W140 + L235
G196 + T334
V264 + S376

G7 + P45
W48 + H321
K72 + G133
W140 + V238
G196 + D337
V264 + D377

G7 + W48
W48 + S323
K72 + T134
W140 + M246
G196 + Q345
V264 + D379

G7 + G50
W48 + H324
K72 + W140
W140 + M248
G196 + G346
V264 + Y382

G7 + T51
W48 + F328
K72 + G142
W140 + L250
G196 + G348
V264 + S383

G7 + N54
W48 + T334
K72 + G149
W140 + G255
G196 + T355
V264 + Q385

G7 + V56
W48 + D337
K72 + T165
W140 + Q256
G196 + S376
V264 + K391

G7 + A60
W48 + Q345
K72 + W167
W140 + A263
G196 + D377
V264 + K393

G7 + K72
W48 + G346
K72 + R171
W140 + V264
G196 + D379
V264 + Q395

G7 + R87
W48 + G348
K72 + Q172
W140 + A265
G196 + Y382
A265 + Y267

G7 + Q98
W48 + T355
K72 + L173
W140 + Y267
G196 + S383
A265 + N270

G7 + M105
W48 + S376
K72 + A174
W140 + N270
G196 + Q385
A265 + G273

G7 + G109
W48 + D377
K72 + G184
W140 + G273
G196 + K391
A265 + S280

G7 + F113
W48 + D379
K72 + T193
W140 + S280
G196 + K393
A265 + T285

G7 + R116
W48 + Y382
K72 + N195
W140 + T285
G196 + Q395
A265 + M286

G7 + Q118
W48 + S383
K72 + G196
W140 + M286
A204 + V206
A265 + F289

G7 + Q125
W48 + Q385
K72 + A204
W140 + F289
A204 + P211
A265 + V291

G7 + G133
W48 + K391
K72 + V206
W140 + V291
A204 + I214
A265 + Q299

G7 + T134
W48 + K393
K72 + P211
W140 + Q299
A204 + V215
A265 + S304

G7 + W140
W48 + Q395
K72 + I214
W140 + S304
A204 + L217
A265 + R320

G7 + G142
G50 + T51
K72 + V215
W140 + R320
A204 + L219
A265 + H321

G7 + G149
G50 + N54
K72 + L217
W140 + H321
A204 + L228
A265 + S323

G7 + T165
G50 + V56
K72 + L219
W140 + S323
A204 + L235
A265 + H324

G7 + W167
G50 + A60
K72 + L228
W140 + H324
A204 + V238
A265 + F328

G7 + R171
G50 + K72
K72 + L235
W140 + F328
A204 + M246
A265 + T334

G7 + Q172
G50 + R87
K72 + V238
W140 + T334
A204 + M248
A265 + D337

G7 + L173
G50 + Q98
K72 + M246
W140 + D337
A204 + L250
A265 + Q345

G7 + A174
G50 + M105
K72 + M248
W140 + Q345
A204 + G255
A265 + G346

G7 + G184
G50 + G109
K72 + L250
W140 + G346
A204 + Q256
A265 + G348

G7 + T193
G50 + F113
K72 + G255
W140 + G348
A204 + A263
A265 + T355

G7 + N195
G50 + R116
K72 + Q256
W140 + T355
A204 + V264
A265 + S376

G7 + G196
G50 + Q118
K72 + A263
W140 + S376
A204 + A265
A265 + D377

G7 + A204
G50 + Q125
K72 + V264
W140 + D377
A204 + Y267
A265 + D379

G7 + V206
G50 + G133
K72 + A265
W140 + D379
A204 + N270
A265 + Y382

G7 + P211
G50 + T134
K72 + Y267
W140 + Y382
A204 + G273
A265 + S383

G7 + I214
G50 + W140
K72 + N270
W140 + S383
A204 + S280
A265 + Q385

G7 + V215
G50 + G142
K72 + G273
W140 + Q385
A204 + T285
A265 + K391

G7 + L217
G50 + G149
K72 + S280
W140 + K391
A204 + M286
A265 + K393

G7 + L219
G50 + T165
K72 + T285
W140 + K393
A204 + F289
A265 + Q395

G7 + L228
G50 + W167
K72 + M286
W140 + Q395
A204 + V291
Y267 + N270

G7 + L235
G50 + R171
K72 + F289
G142 + G149
A204 + Q299
Y267 + G273

G7 + V238
G50 + Q172
K72 + V291
G142 + T165
A204 + S304
Y267 + S280

G7 + M246
G50 + L173
K72 + Q299
G142 + W167
A204 + R320
Y267 + T285

G7 + M248
G50 + A174
K72 + S304
G142 + R171
A204 + H321
Y267 + M286

G7 + L250
G50 + G184
K72 + R320
G142 + Q172
A204 + S323
Y267 + F289

G7 + G255
G50 + T193
K72 + H321
G142 + L173
A204 + H324
Y267 + V291

G7 + Q256
G50 + N195
K72 + S323
G142 + A174
A204 + F328
Y267 + Q299

G7 + A263
G50 + G196
K72 + H324
G142 + G184
A204 + T334
Y267 + S304

G7 + V264
G50 + A204
K72 + F328
G142 + T193
A204 + D337
Y267 + R320

G7 + A265
G50 + V206
K72 + T334
G142 + N195
A204 + Q345
Y267 + H321

G7 + Y267
G50 + P211
K72 + D337
G142 + G196
A204 + G346
Y267 + S323

G7 + N270
G50 + I214
K72 + Q345
G142 + A204
A204 + G348
Y267 + H324

G7 + G273
G50 + V215
K72 + G346
G142 + V206
A204 + T355
Y267 + F328

G7 + S280
G50 + L217
K72 + G348
G142 + P211
A204 + S376
Y267 + T334

G7 + T285
G50 + L219
K72 + T355
G142 + I214
A204 + D377
Y267 + D337

G7 + M286
G50 + L228
K72 + S376
G142 + V215
A204 + D379
Y267 + Q345

G7 + F289
G50 + L235
K72 + D377
G142 + L217
A204 + Y382
Y267 + G346

G7 + V291
G50 + V238
K72 + D379
G142 + L219
A204 + S383
Y267 + G348

G7 + Q299
G50 + M246
K72 + Y382
G142 + L228
A204 + Q385
Y267 + T355

G7 + S304
G50 + M248
K72 + S383
G142 + L235
A204 + K391
Y267 + S376

G7 + R320
G50 + L250
K72 + Q385
G142 + V238
A204 + K393
Y267 + D377

G7 + H321
G50 + G255
K72 + K391
G142 + M246
A204 + Q395
Y267 + D379

G7 + S323
G50 + Q256
K72 + K393
G142 + M248
V206 + P211
Y267 + Y382

G7 + H324
G50 + A263
K72 + Q395
G142 + L250
V206 + I214
Y267 + S383

G7 + F328
G50 + V264
R87 + Q98
G142 + G255
V206 + V215
Y267 + Q385

G7 + T334
G50 + A265
R87 + M105
G142 + Q256
V206 + L217
Y267 + K391

G7 + D337
G50 + Y267
R87 + G109
G142 + A263
V206 + L219
Y267 + K393

G7 + Q345
G50 + N270
R87 + F113
G142 + V264
V206 + L228
Y267 + Q395

G7 + G346
G50 + G273
R87 + R116
G142 + A265
V206 + L235
N270 + G273

G7 + G348
G50 + S280
R87 + Q118
G142 + Y267
V206 + V238
N270 + S280

G7 + T355
G50 + T285
R87 + Q125
G142 + N270
V206 + M246
N270 + T285

G7 + S376
G50 + M286
R87 + G133
G142 + G273
V206 + M248
N270 + M286

G7 + D377
G50 + F289
R87 + T134
G142 + S280
V206 + L250
N270 + F289

G7 + D379
G50 + V291
R87 + W140
G142 + T285
V206 + G255
N270 + V291

G7 + Y382
G50 + Q299
R87 + G142
G142 + M286
V206 + Q256
N270 + Q299

G7 + S383
G50 + S304
R87 + G149
G142 + F289
V206 + A263
N270 + S304

G7 + Q385
G50 + R320
R87 + T165
G142 + V291
V206 + V264
N270 + R320

G7 + K391
G50 + H321
R87 + W167
G142 + Q299
V206 + A265
N270 + H321

G7 + K393
G50 + S323
R87 + R171
G142 + S304
V206 + Y267
N270 + S323

G7 + Q395
G50 + H324
R87 + Q172
G142 + R320
V206 + N270
N270 + H324

Q11 + N16
G50 + F328
R87 + L173
G142 + H321
V206 + G273
N270 + F328

Q11 + V17
G50 + T334
R87 + A174
G142 + S323
V206 + S280
N270 + T334

Q11 + Q32
G50 + D337
R87 + G184
G142 + H324
V206 + T285
N270 + D337

Q11 + A37
G50 + Q345
R87 + T193
G142 + F328
V206 + M286
N270 + Q345

Q11 + T40
G50 + G346
R87 + N195
G142 + T334
V206 + F289
N270 + G346

Q11 + P45
G50 + G348
R87 + G196
G142 + D337
V206 + V291
N270 + G348

Q11 + W48
G50 + T355
R87 + A204
G142 + Q345
V206 + Q299
N270 + T355

Q11 + G50
G50 + S376
R87 + V206
G142 + G346
V206 + S304
N270 + S376

Q11 + T51
G50 + D377
R87 + P211
G142 + G348
V206 + R320
N270 + D377

Q11 + N54
G50 + D379
R87 + I214
G142 + T355
V206 + H321
N270 + D379

Q11 + V56
G50 + Y382
R87 + V215
G142 + S376
V206 + S323
N270 + Y382

Q11 + A60
G50 + S383
R87 + L217
G142 + D377
V206 + H324
N270 + S383

Q11 + K72
G50 + Q385
R87 + L219
G142 + D379
V206 + F328
N270 + Q385

Q11 + R87
G50 + K391
R87 + L228
G142 + Y382
V206 + T334
N270 + K391

Q11 + Q98
G50 + K393
R87 + L235
G142 + S383
V206 + D337
N270 + K393

Q11 + M105
G50 + Q395
R87 + V238
G142 + Q385
V206 + Q345
N270 + Q395

Q11 + G109
T51 + N54
R87 + M246
G142 + K391
V206 + G346
G273 + S280

Q11 + F113
T51 + V56
R87 + M248
G142 + K393
V206 + G348
G273 + T285

Q11 + R116
T51 + A60
R87 + L250
G142 + Q395
V206 + T355
G273 + M286

Q11 + Q118
T51 + K72
R87 + G255
G149 + T165
V206 + S376
G273 + F289

Q11 + Q125
T51 + R87
R87 + Q256
G149 + W167
V206 + D377
G273 + V291

Q11 + G133
T51 + Q98
R87 + A263
G149 + R171
V206 + D379
G273 + Q299

Q11 + T134
T51 + M105
R87 + V264
G149 + Q172
V206 + Y382
G273 + S304

Q11 + W140
T51 + G109
R87 + A265
G149 + L173
V206 + S383
G273 + R320

Q11 + G142
T51 + F113
R87 + Y267
G149 + A174
V206 + Q385
G273 + H321

Q11 + G149
T51 + R116
R87 + N270
G149 + G184
V206 + K391
G273 + S323

Q11 + T165
T51 + Q118
R87 + G273
G149 + T193
V206 + K393
G273 + H324

Q11 + W167
T51 + Q125
R87 + S280
G149 + N195
V206 + Q395
G273 + F328

Q11 + R171
T51 + G133
R87 + T285
G149 + G196
P211 + I214
G273 + T334

Q11 + Q172
T51 + T134
R87 + M286
G149 + A204
P211 + V215
G273 + D337

Q11 + L173
T51 + W140
R87 + F289
G149 + V206
P211 + L217
G273 + Q345

Q11 + A174
T51 + G142
R87 + V291
G149 + P211
P211 + L219
G273 + G346

Q11 + G184
T51 + G149
R87 + Q299
G149 + I214
P211 + L228
G273 + G348

Q11 + T193
T51 + T165
R87 + S304
G149 + V215
P211 + L235
G273 + T355

Q11 + N195
T51 + W167
R87 + R320
G149 + L217
P211 + V238
G273 + S376

Q11 + G196
T51 + R171
R87 + H321
G149 + L219
P211 + M246
G273 + D377

Q11 + A204
T51 + Q172
R87 + S323
G149 + L228
P211 + M248
G273 + D379

Q11 + V206
T51 + L173
R87 + H324
G149 + L235
P211 + L250
G273 + Y382

Q11 + P211
T51 + A174
R87 + F328
G149 + V238
P211 + G255
G273 + S383

Q11 + I214
T51 + G184
R87 + T334
G149 + M246
P211 + Q256
G273 + Q385

Q11 + V215
T51 + T193
R87 + D337
G149 + M248
P211 + A263
G273 + K391

Q11 + L217
T51 + N195
R87 + Q345
G149 + L250
P211 + V264
G273 + K393

Q11 + L219
T51 + G196
R87 + G346
G149 + G255
P211 + A265
G273 + Q395

Q11 + L228
T51 + A204
R87 + G348
G149 + Q256
P211 + Y267
S280 + T285

Q11 + L235
T51 + V206
R87 + T355
G149 + A263
P211 + N270
S280 + M286

Q11 + V238
T51 + P211
R87 + S376
G149 + V264
P211 + G273
S280 + F289

Q11 + M246
T51 + I214
R87 + D377
G149 + A265
P211 + S280
S280 + V291

Q11 + M248
T51 + V215
R87 + D379
G149 + Y267
P211 + T285
S280 + Q299

Q11 + L250
T51 + L217
R87 + Y382
G149 + N270
P211 + M286
S280 + S304

Q11 + G255
T51 + L219
R87 + S383
G149 + G273
P211 + F289
S280 + R320

Q11 + Q256
T51 + L228
R87 + Q385
G149 + S280
P211 + V291
S280 + H321

Q11 + A263
T51 + L235
R87 + K391
G149 + T285
P211 + Q299
S280 + S323

Q11 + V264
T51 + V238
R87 + K393
G149 + M286
P211 + S304
S280 + H324

Q11 + A265
T51 + M246
R87 + Q395
G149 + F289
P211 + R320
S280 + F328

Q11 + Y267
T51 + M248
Q98 + M105
G149 + V291
P211 + H321
S280 + T334

Q11 + N270
T51 + L250
Q98 + G109
G149 + Q299
P211 + S323
S280 + D337

Q11 + G273
T51 + G255
Q98 + F113
G149 + S304
P211 + H324
S280 + Q345

Q11 + S280
T51 + Q256
Q98 + R116
G149 + R320
P211 + F328
S280 + G346

Q11 + T285
T51 + A263
Q98 + Q118
G149 + H321
P211 + T334
S280 + G348

Q11 + M286
T51 + V264
Q98 + Q125
G149 + S323
P211 + D337
S280 + T355

Q11 + F289
T51 + A265
Q98 + G133
G149 + H324
P211 + Q345
S280 + S376

Q11 + V291
T51 + Y267
Q98 + T134
G149 + F328
P211 + G346
S280 + D377

Q11 + Q299
T51 + N270
Q98 + W140
G149 + T334
P211 + G348
S280 + D379

Q11 + S304
T51 + G273
Q98 + G142
G149 + D337
P211 + T355
S280 + Y382

Q11 + R320
T51 + S280
Q98 + G149
G149 + Q345
P211 + S376
S280 + S383

Q11 + H321
T51 + T285
Q98 + T165
G149 + G346
P211 + D377
S280 + Q385

Q11 + S323
T51 + M286
Q98 + W167
G149 + G348
P211 + D379
S280 + K391

Q11 + H324
T51 + F289
Q98 + R171
G149 + T355
P211 + Y382
S280 + K393

Q11 + F328
T51 + V291
Q98 + Q172
G149 + S376
P211 + S383
S280 + Q395

Q11 + T334
T51 + Q299
Q98 + L173
G149 + D377
P211 + Q385
T285 + M286

Q11 + D337
T51 + S304
Q98 + A174
G149 + D379
P211 + K391
T285 + F289

Q11 + Q345
T51 + R320
Q98 + G184
G149 + Y382
P211 + K393
T285 + V291

Q11 + G346
T51 + H321
Q98 + T193
G149 + S383
P211 + Q395
T285 + Q299

Q11 + G348
T51 + S323
Q98 + N195
G149 + Q385
I214 + V215
T285 + S304

Q11 + T355
T51 + H324
Q98 + G196
G149 + K391
I214 + L217
T285 + R320

Q11 + S376
T51 + F328
Q98 + A204
G149 + K393
I214 + L219
T285 + H321

Q11 + D377
T51 + T334
Q98 + V206
G149 + Q395
I214 + L228
T285 + S323

Q11 + D379
T51 + D337
Q98 + P211
T165 + W167
I214 + L235
T285 + H324

Q11 + Y382
T51 + Q345
Q98 + I214
T165 + R171
I214 + V238
T285 + F328

Q11 + S383
T51 + G346
Q98 + V215
T165 + Q172
I214 + M246
T285 + T334

Q11 + Q385
T51 + G348
Q98 + L217
T165 + L173
I214 + M248
T285 + D337

Q11 + K391
T51 + T355
Q98 + L219
T165 + A174
I214 + L250
T285 + Q345

Q11 + K393
T51 + S376
Q98 + L228
T165 + G184
I214 + G255
T285 + G346

Q11 + Q395
T51 + D377
Q98 + L235
T165 + T193
I214 + Q256
T285 + G348

N16 + V17
T51 + D379
Q98 + V238
T165 + N195
I214 + A263
T285 + T355

N16 + Q32
T51 + Y382
Q98 + M246
T165 + G196
I214 + V264
T285 + S376

N16 + A37
T51 + S383
Q98 + M248
T165 + A204
I214 + A265
T285 + D377

N16 + T40
T51 + Q385
Q98 + L250
T165 + V206
I214 + Y267
T285 + D379

N16 + P45
T51 + K391
Q98 + G255
T165 + P211
I214 + N270
T285 + Y382

N16 + W48
T51 + K393
Q98 + Q256
T165 + I214
I214 + G273
T285 + S383

N16 + G50
T51 + Q395
Q98 + A263
T165 + V215
I214 + S280
T285 + Q385

N16 + T51
N54 + V56
Q98 + V264
T165 + L217
I214 + T285
T285 + K391

N16 + N54
N54 + A60
Q98 + A265
T165 + L219
I214 + M286
T285 + K393

N16 + V56
N54 + K72
Q98 + Y267
T165 + L228
I214 + F289
T285 + Q395

N16 + A60
N54 + R87
Q98 + N270
T165 + L235
I214 + V291
M286 + F289

N16 + K72
N54 + Q98
Q98 + G273
T165 + V238
I214 + Q299
M286 + V291

N16 + R87
N54 + M105
Q98 + S280
T165 + M246
I214 + S304
M286 + Q299

N16 + Q98
N54 + G109
Q98 + T285
T165 + M248
I214 + R320
M286 + S304

N16 + M105
N54 + F113
Q98 + M286
T165 + L250
I214 + H321
M286 + R320

N16 + G109
N54 + R116
Q98 + F289
T165 + G255
I214 + S323
M286 + H321

N16 + F113
N54 + Q118
Q98 + V291
T165 + Q256
I214 + H324
M286 + S323

N16 + R116
N54 + Q125
Q98 + Q299
T165 + A263
I214 + F328
M286 + H324

N16 + Q118
N54 + G133
Q98 + S304
T165 + V264
I214 + T334
M286 + F328

N16 + Q125
N54 + T134
Q98 + R320
T165 + A265
I214 + D337
M286 + T334

N16 + G133
N54 + W140
Q98 + H321
T165 + Y267
I214 + Q345
M286 + D337

N16 + T134
N54 + G142
Q98 + S323
T165 + N270
I214 + G346
M286 + Q345

N16 + W140
N54 + G149
Q98 + H324
T165 + G273
I214 + G348
M286 + G346

N16 + G142
N54 + T165
Q98 + F328
T165 + S280
I214 + T355
M286 + G348

N16 + G149
N54 + W167
Q98 + T334
T165 + T285
I214 + S376
M286 + T355

N16 + T165
N54 + R171
Q98 + D337
T165 + M286
I214 + D377
M286 + S376

N16 + W167
N54 + Q172
Q98 + Q345
T165 + F289
I214 + D379
M286 + D377

N16 + R171
N54 + L173
Q98 + G346
T165 + V291
I214 + Y382
M286 + D379

N16 + Q172
N54 + A174
Q98 + G348
T165 + Q299
I214 + S383
M286 + Y382

N16 + L173
N54 + G184
Q98 + T355
T165 + S304
I214 + Q385
M286 + S383

N16 + A174
N54 + T193
Q98 + S376
T165 + R320
I214 + K391
M286 + Q385

N16 + G184
N54 + N195
Q98 + D377
T165 + H321
I214 + K393
M286 + K391

N16 + T193
N54 + G196
Q98 + D379
T165 + S323
I214 + Q395
M286 + K393

N16 + N195
N54 + A204
Q98 + Y382
T165 + H324
V215 + L217
M286 + Q395

N16 + G196
N54 + V206
Q98 + S383
T165 + F328
V215 + L219
F289 + V291

N16 + A204
N54 + P211
Q98 + Q385
T165 + T334
V215 + L228
F289 + Q299

N16 + V206
N54 + I214
Q98 + K391
T165 + D337
V215 + L235
F289 + S304

N16 + P211
N54 + V215
Q98 + K393
T165 + Q345
V215 + V238
F289 + R320

N16 + I214
N54 + L217
Q98 + Q395
T165 + G346
V215 + M246
F289 + H321

N16 + V215
N54 + L219
M105 + G109
T165 + G348
V215 + M248
F289 + S323

N16 + L217
N54 + L228
M105 + F113
T165 + T355
V215 + L250
F289 + H324

N16 + L219
N54 + L235
M105 + R116
T165 + S376
V215 + G255
F289 + F328

N16 + L228
N54 + V238
M105 + Q118
T165 + D377
V215 + Q256
F289 + T334

N16 + L235
N54 + M246
M105 + Q125
T165 + D379
V215 + A263
F289 + D337

N16 + V238
N54 + M248
M105 + G133
T165 + Y382
V215 + V264
F289 + Q345

N16 + M246
N54 + L250
M105 + T134
T165 + S383
V215 + A265
F289 + G346

N16 + M248
N54 + G255
M105 + W140
T165 + Q385
V215 + Y267
F289 + G348

N16 + L250
N54 + Q256
M105 + G142
T165 + K391
V215 + N270
F289 + T355

N16 + G255
N54 + A263
M105 + G149
T165 + K393
V215 + G273
F289 + S376

N16 + Q256
N54 + V264
M105 + T165
T165 + Q395
V215 + S280
F289 + D377

N16 + A263
N54 + A265
M105 + W167
W167 + R171
V215 + T285
F289 + D379

N16 + V264
N54 + Y267
M105 + R171
W167 + Q172
V215 + M286
F289 + Y382

N16 + A265
N54 + N270
M105 + Q172
W167 + L173
V215 + F289
F289 + S383

N16 + Y267
N54 + G273
M105 + L173
W167 + A174
V215 + V291
F289 + Q385

N16 + N270
N54 + S280
M105 + A174
W167 + G184
V215 + Q299
F289 + K391

N16 + G273
Q118 + V215
M105 + G184
W167 + T193
V215 + S304
F289 + K393

N16 + S280
Q118 + L217
M105 + T193
W167 + N195
V215 + R320
F289 + Q395

N16 + T285
Q118 + L219
M105 + N195
W167 + G196
V215 + H321
V291 + Q299

N16 + M286
Q118 + L228
M105 + G196
W167 + A204
V215 + S323
V291 + S304

N16 + F289
Q118 + L235
M105 + A204
W167 + V206
V215 + H324
V291 + R320

N16 + V291
Q118 + V238
M105 + V206
W167 + P211
V215 + F328
V291 + H321

N16 + Q299
Q118 + M246
M105 + P211
W167 + I214
V215 + T334
V291 + S323

N16 + S304
Q118 + M248
M105 + I214
W167 + V215
V215 + D337
V291 + H324

N16 + R320
Q118 + L250
M105 + V215
W167 + L217
V215 + Q345
V291 + F328

N16 + H321
Q118 + G255
M105 + L217
W167 + L219
V215 + G346
V291 + T334

N16 + S323
Q118 + Q256
M105 + L219
W167 + L228
V215 + G348
V291 + D337

N16 + H324
Q118 + A263
M105 + L228
W167 + L235
V215 + T355
V291 + Q345

N16 + F328
Q118 + V264
M105 + L235
W167 + V238
V215 + S376
V291 + G346

N16 + T334
Q118 + A265
M105 + V238
W167 + M246
V215 + D377
V291 + G348

N16 + D337
Q118 + Y267
M105 + M246
W167 + M248
V215 + D379
V291 + T355

N16 + Q345
Q118 + N270
M105 + M248
W167 + L250
V215 + Y382
V291 + S376

N16 + G346
Q118 + G273
M105 + L250
W167 + G255
V215 + S383
V291 + D377

N16 + G348
Q118 + S280
M105 + G255
W167 + Q256
V215 + Q385
V291 + D379

N16 + T355
Q118 + T285
M105 + Q256
W167 + A263
V215 + K391
V291 + Y382

N16 + S376
Q118 + M286
M105 + A263
W167 + V264
V215 + K393
V291 + S383

N16 + D377
Q118 + F289
M105 + V264
W167 + A265
V215 + Q395
V291 + Q385

N16 + D379
Q118 + V291
M105 + A265
W167 + Y267
L217 + L219
V291 + K391

N16 + Y382
Q118 + Q299
M105 + Y267
W167 + N270
L217 + L228
V291 + K393

N16 + S383
Q118 + S304
M105 + N270
W167 + G273
L217 + L235
V291 + Q395

N16 + Q385
Q118 + R320
M105 + G273
W167 + S280
L217 + V238
Q299 + S304

N16 + K391
Q118 + H321
M105 + S280
W167 + T285
L217 + M246
Q299 + R320

N16 + K393
Q118 + S323
M105 + T285
W167 + M286
L217 + M248
Q299 + H321

N16 + Q395
Q118 + H324
M105 + M286
W167 + F289
L217 + L250
Q299 + S323

V17 + Q32
Q118 + F328
M105 + F289
W167 + V291
L217 + G255
Q299 + H324

V17 + A37
Q118 + T334
M105 + V291
W167 + Q299
L217 + Q256
Q299 + F328

V17 + T40
Q118 + D337
M105 + Q299
W167 + S304
L217 + A263
Q299 + T334

V17 + P45
Q118 + Q345
M105 + S304
W167 + R320
L217 + V264
Q299 + D337

V17 + W48
Q118 + G346
M105 + R320
W167 + H321
L217 + A265
Q299 + Q345

V17 + G50
Q118 + G348
M105 + H321
W167 + S323
L217 + Y267
Q299 + G346

V17 + T51
Q118 + T355
M105 + S323
W167 + H324
L217 + N270
Q299 + G348

V17 + N54
Q118 + S376
M105 + H324
W167 + F328
L217 + G273
Q299 + T355

V17 + V56
Q118 + D377
M105 + F328
W167 + T334
L217 + S280
Q299 + S376

V17 + A60
Q118 + D379
M105 + T334
W167 + D337
L217 + T285
Q299 + D377

V17 + K72
Q118 + Y382
M105 + D337
W167 + Q345
L217 + M286
Q299 + D379

V17 + R87
Q118 + S383
M105 + Q345
W167 + G346
L217 + F289
Q299 + Y382

V17 + Q98
Q118 + Q385
M105 + G346
W167 + G348
L217 + V291
Q299 + S383

V17 + M105
Q118 + K391
M105 + G348
W167 + T355
L217 + Q299
Q299 + Q385

V17 + G109
Q118 + K393
M105 + T355
W167 + S376
L217 + S304
Q299 + K391

V17 + F113
Q118 + Q395
M105 + S376
W167 + D377
L217 + R320
Q299 + K393

V17 + R116
Q125 + G133
M105 + D377
W167 + D379
L217 + H321
Q299 + Q395

V17 + Q118
Q125 + T134
M105 + D379
W167 + Y382
L217 + S323
S304 + R320

V17 + Q125
Q125 + W140
M105 + Y382
W167 + S383
L217 + H324
S304 + H321

V17 + G133
Q125 + G142
M105 + S383
W167 + Q385
L217 + F328
S304 + S323

V17 + T134
Q125 + G149
M105 + Q385
W167 + K391
L217 + T334
S304 + H324

V17 + W140
Q125 + T165
M105 + K391
W167 + K393
L217 + D337
S304 + F328

V17 + G142
Q125 + W167
M105 + K393
W167 + Q395
L217 + Q345
S304 + T334

V17 + G149
Q125 + R171
M105 + Q395
R171 + Q172
L217 + G346
S304 + D337

V17 + T165
Q125 + Q172
G109 + F113
R171 + L173
L217 + G348
S304 + Q345

V17 + W167
Q125 + L173
G109 + R116
R171 + A174
L217 + T355
S304 + G346

V17 + R171
Q125 + A174
G109 + Q118
R171 + G184
L217 + S376
S304 + G348

V17 + Q172
Q125 + G184
G109 + Q125
R171 + T193
L217 + D377
S304 + T355

V17 + L173
Q125 + T193
G109 + G133
R171 + N195
L217 + D379
S304 + S376

V17 + A174
Q125 + N195
G109 + T134
R171 + G196
L217 + Y382
S304 + D377

V17 + G184
Q125 + G196
G109 + W140
R171 + A204
L217 + S383
S304 + D379

V17 + T193
Q125 + A204
G109 + G142
R171 + V206
L217 + Q385
S304 + Y382

V17 + N195
Q125 + V206
G109 + G149
R171 + P211
L217 + K391
S304 + S383

V17 + G196
Q125 + P211
G109 + T165
R171 + I214
L217 + K393
S304 + Q385

V17 + A204
Q125 + I214
G109 + W167
R171 + V215
L217 + Q395
S304 + K391

V17 + V206
Q125 + V215
G109 + R171
R171 + L217
L219 + L228
S304 + K393

V17 + P211
Q125 + L217
G109 + Q172
R171 + L219
L219 + L235
S304 + Q395

V17 + I214
Q125 + L219
G109 + L173
R171 + L228
L219 + V238
R320 + H321

V17 + V215
Q125 + L228
G109 + A174
R171 + L235
L219 + M246
R320 + S323

V17 + L217
Q125 + L235
G109 + G184
R171 + V238
L219 + M248
R320 + H324

V17 + L219
Q125 + V238
G109 + T193
R171 + M246
L219 + L250
R320 + F328

V17 + L228
Q125 + M246
G109 + N195
R171 + M248
L219 + G255
R320 + T334

V17 + L235
Q125 + M248
G109 + G196
R171 + L250
L219 + Q256
R320 + D337

V17 + V238
Q125 + L250
G109 + A204
R171 + G255
L219 + A263
R320 + Q345

V17 + M246
A37 + Q395
G109 + V206
R171 + Q256
L219 + V264
R320 + G346

V17 + M248
T40 + P45
G109 + P211
R171 + A263
L219 + A265
R320 + G348

V17 + L250
T40 + W48
G109 + I214
R171 + V264
L219 + Y267
R320 + T355

V17 + G255
T40 + G50
G109 + V215
R171 + A265
L219 + N270
R320 + S376

V17 + Q256
T40 + T51
G109 + L217
R171 + Y267
L219 + G273
R320 + D377

V17 + A263
T40 + N54
G109 + L219
R171 + N270
L219 + S280
R320 + D379

V17 + V264
T40 + V56
G109 + L228
R171 + G273
L219 + T285
R320 + Y382

V17 + A265
T40 + A60
G109 + L235
R171 + S280
L219 + M286
R320 + S383

V17 + Y267
T40 + K72
G109 + V238
R171 + T285
L219 + F289
R320 + Q385

V17 + N270
T40 + R87
G109 + M246
R171 + M286
L219 + V291
R320 + K391

V17 + G273
T40 + Q98
G109 + M248
R171 + F289
L219 + Q299
R320 + K393

V17 + S280
T40 + M105
G109 + L250
R171 + V291
L219 + S304
R320 + Q395

V17 + T285
T40 + G109
G109 + G255
R171 + Q299
L219 + R320
H321 + S323

V17 + M286
T40 + F113
G109 + Q256
R171 + S304
L219 + H321
H321 + H324

V17 + F289
T40 + R116
G109 + A263
R171 + R320
L219 + S323
H321 + F328

V17 + V291
T40 + Q118
G109 + V264
R171 + H321
L219 + H324
H321 + T334

V17 + Q299
T40 + Q125
G109 + A265
R171 + S323
L219 + F328
H321 + D337

V17 + S304
T40 + G133
G109 + Y267
R171 + H324
L219 + T334
H321 + Q345

V17 + R320
T40 + T134
G109 + N270
R171 + F328
L219 + D337
H321 + G346

V17 + H321
T40 + W140
G109 + G273
R171 + T334
L219 + Q345
H321 + G348

V17 + S323
T40 + G142
G109 + S280
R171 + D337
L219 + G346
H321 + T355

V17 + H324
T40 + G149
G109 + T285
R171 + Q345
L219 + G348
H321 + S376

V17 + F328
T40 + T165
G109 + M286
R171 + G346
L219 + T355
H321 + D377

V17 + T334
T40 + W167
G109 + F289
R171 + G348
L219 + S376
H321 + D379

V17 + D337
T40 + R171
G109 + V291
R171 + T355
L219 + D377
H321 + Y382

V17 + Q345
T40 + Q172
G109 + Q299
R171 + S376
L219 + D379
H321 + S383

V17 + G346
T40 + L173
G109 + S304
R171 + D377
L219 + Y382
H321 + Q385

V17 + G348
T40 + A174
G109 + R320
R171 + D379
L219 + S383
H321 + K391

V17 + T355
T40 + G184
G109 + H321
R171 + Y382
L219 + Q385
H321 + K393

V17 + S376
T40 + T193
G109 + S323
R171 + S383
L219 + K391
H321 + Q395

V17 + D377
T40 + N195
G109 + H324
R171 + Q385
L219 + K393
S323 + H324

V17 + D379
T40 + G196
G109 + F328
R171 + K391
L219 + Q395
S323 + F328

V17 + Y382
T40 + A204
G109 + T334
R171 + K393
L228 + L235
S323 + T334

V17 + S383
T40 + V206
G109 + D337
R171 + Q395
L228 + V238
S323 + D337

V17 + Q385
T40 + P211
G109 + Q345
Q172 + L173
L228 + M246
S323 + Q345

V17 + K391
T40 + I214
G109 + G346
Q172 + A174
L228 + M248
S323 + G346

V17 + K393
T40 + V215
G109 + G348
Q172 + G184
L228 + L250
S323 + G348

V17 + Q395
H1 + Q169
G109 + T355
Q172 + T193
L228 + G255
S323 + T355

Q32 + A37
H1 + A186
G109 + S376
Q172 + N195
L228 + Q256
S323 + S376

Q32 + T40
H1 + E190
G109 + D377
Q172 + G196
L228 + A263
S323 + D377

Q32 + P45
H1 + A225
G109 + D379
Q172 + A204
L228 + V264
S323 + D379

Q32 + W48
H1 + K242
G109 + Y382
Q172 + V206
L228 + A265
S323 + Y382

Q32 + G50
H1 + S244
G109 + S383
Q172 + P211
L228 + Y267
S323 + S383

Q32 + T51
H1 + N260
G109 + Q385
Q172 + I214
L228 + N270
S323 + Q385

Q32 + N54
H1 + K269
G109 + K391
Q172 + V215
L228 + G273
S323 + K391

Q32 + V56
H1 + W284
G109 + K393
Q172 + L217
L228 + S280
S323 + K393

Q32 + A60
H1 + Y295
G109 + Q395
Q172 + L219
L228 + T285
S323 + Q395

Q32 + K72
H1 + V326
F113 + R116
Q172 + L228
L228 + M286
H324 + F328

Q32 + R87
T5 + Q169
F113 + Q118
Q172 + L235
L228 + F289
H324 + T334

Q32 + Q98
T5 + A186
F113 + Q125
Q172 + V238
L228 + V291
H324 + D337

Q32 + M105
T5 + E190
F113 + G133
Q172 + M246
L228 + Q299
H324 + Q345

Q32 + G109
T5 + A225
F113 + T134
Q172 + M248
L228 + S304
H324 + G346

Q32 + F113
T5 + K242
F113 + W140
Q172 + L250
L228 + R320
H324 + G348

Q32 + R116
T5 + S244
F113 + G142
Q172 + G255
L228 + H321
H324 + T355

Q32 + Q118
T5 + N260
F113 + G149
Q172 + Q256
L228 + S323
H324 + S376

Q32 + Q125
T5 + K269
F113 + T165
Q172 + A263
L228 + H324
H324 + D377

Q32 + G133
T5 + W284
F113 + W167
Q172 + V264
L228 + F328
H324 + D379

Q32 + T134
T5 + Y295
F113 + R171
Q172 + A265
L228 + T334
H324 + Y382

Q32 + W140
T5 + V326
F113 + Q172
Q172 + Y267
L228 + D337
H324 + S383

Q32 + G142
G7 + Q169
F113 + L173
Q172 + N270
L228 + Q345
H324 + Q385

Q32 + G149
G7 + A186
F113 + A174
Q172 + G273
L228 + G346
H324 + K391

Q32 + T165
G7 + E190
F113 + G184
Q172 + S280
L228 + G348
H324 + K393

Q32 + W167
G7 + A225
F113 + T193
Q172 + T285
L228 + T355
H324 + Q395

Q32 + R171
G7 + K242
F113 + N195
Q172 + M286
L228 + S376
F328 + T334

Q32 + Q172
G7 + S244
F113 + G196
Q172 + F289
L228 + D377
F328 + D337

Q32 + L173
G7 + N260
F113 + A204
Q172 + V291
L228 + D379
F328 + Q345

Q32 + A174
G7 + K269
F113 + V206
Q172 + Q299
L228 + Y382
F328 + G346

Q32 + G184
G7 + W284
F113 + P211
Q172 + S304
L228 + S383
F328 + G348

Q32 + T193
G7 + Y295
F113 + I214
Q172 + R320
L228 + Q385
F328 + T355

Q32 + N195
G7 + V326
F113 + V215
Q172 + H321
L228 + K391
F328 + S376

Q32 + G196
Q11 + Q169
F113 + L217
Q172 + S323
L228 + K393
F328 + D377

Q32 + A204
Q11 + A186
F113 + L219
Q172 + H324
L228 + Q395
F328 + D379

Q32 + V206
Q11 + E190
F113 + L228
Q172 + F328
L235 + V238
F328 + Y382

Q32 + P211
Q11 + A225
F113 + L235
Q172 + T334
L235 + M246
F328 + S383

Q32 + I214
Q11 + K242
F113 + V238
Q172 + D337
L235 + M248
F328 + Q385

Q32 + V215
Q11 + S244
F113 + M246
Q172 + Q345
L235 + L250
F328 + K391

Q32 + L217
Q11 + N260
F113 + M248
Q172 + G346
L235 + G255
F328 + K393

Q32 + L219
Q11 + K269
F113 + L250
Q172 + G348
L235 + Q256
F328 + Q395

Q32 + L228
Q11 + W284
F113 + G255
Q172 + T355
L235 + A263
T334 + D337

Q32 + L235
Q11 + Y295
F113 + Q256
Q172 + S376
L235 + V264
T334 + Q345

Q32 + V238
Q11 + V326
F113 + A263
Q172 + D377
L235 + A265
T334 + G346

Q32 + M246
N16 + Q169
F113 + V264
Q172 + D379
L235 + Y267
T334 + G348

Q32 + M248
N16 + A186
F113 + A265
Q172 + Y382
L235 + N270
T334 + T355

Q32 + L250
N16 + E190
F113 + Y267
Q172 + S383
L235 + G273
T334 + S376

Q32 + G255
N16 + A225
F113 + N270
Q172 + Q385
L235 + S280
T334 + D377

Q32 + Q256
N16 + K242
F113 + G273
Q172 + K391
L235 + T285
T334 + D379

Q32 + A263
N16 + S244
F113 + S280
Q172 + K393
L235 + M286
T334 + Y382

Q32 + V264
N16 + N260
F113 + T285
Q172 + Q395
L235 + F289
T334 + S383

Q32 + A265
N16 + K269
F113 + M286
L173 + A174
L235 + V291
T334 + Q385

Q32 + Y267
N16 + W284
F113 + F289
L173 + G184
L235 + Q299
T334 + K391

Q32 + N270
N16 + Y295
F113 + V291
L173 + T193
L235 + S304
T334 + K393

Q32 + G273
N16 + V326
F113 + Q299
L173 + N195
L235 + R320
T334 + Q395

Q32 + S280
V17 + Q169
F113 + S304
L173 + G196
L235 + H321
D337 + Q345

Q32 + T285
V17 + A186
F113 + R320
L173 + A204
L235 + S323
D337 + G346

Q32 + M286
V17 + E190
F113 + H321
L173 + V206
L235 + H324
D337 + G348

Q32 + F289
V17 + A225
F113 + S323
L173 + P211
L235 + F328
D337 + T355

Q32 + V291
V17 + K242
F113 + H324
L173 + I214
L235 + T334
D337 + S376

Q32 + Q299
V17 + S244
F113 + F328
L173 + V215
L235 + D337
D337 + D377

Q32 + S304
V17 + N260
F113 + T334
L173 + L217
L235 + Q345
D337 + D379

Q32 + R320
V17 + K269
F113 + D337
L173 + L219
L235 + G346
D337 + Y382

Q32 + H321
V17 + W284
F113 + Q345
L173 + L228
L235 + G348
D337 + S383

Q32 + S323
V17 + Y295
F113 + G346
L173 + L235
L235 + T355
D337 + Q385

Q32 + H324
V17 + V326
F113 + G348
L173 + V238
L235 + S376
D337 + K391

Q32 + F328
Q32 + Q169
F113 + T355
L173 + M246
L235 + D377
D337 + K393

Q32 + T334
Q32 + A186
F113 + S376
L173 + M248
L235 + D379
D337 + Q395

Q32 + D337
Q32 + E190
F113 + D377
L173 + L250
L235 + Y382
Q345 + G346

Q32 + Q345
Q32 + A225
F113 + D379
L173 + G255
L235 + S383
Q345 + G348

Q32 + G346
Q32 + K242
F113 + Y382
L173 + Q256
L235 + Q385
Q345 + T355

Q32 + G348
Q32 + S244
F113 + S383
L173 + A263
L235 + K391
Q345 + S376

Q32 + T355
Q32 + N260
F113 + Q385
L173 + V264
L235 + K393
Q345 + D377

Q32 + S376
Q32 + K269
F113 + K391
L173 + A265
L235 + Q395
Q345 + D379

Q32 + D377
Q32 + W284
F113 + K393
L173 + Y267
V238 + M246
Q345 + Y382

Q32 + D379
Q32 + Y295
F113 + Q395
L173 + N270
V238 + M248
Q345 + S383

Q32 + Y382
Q32 + V326
R116 + Q118
L173 + G273
V238 + L250
Q345 + Q385

Q32 + S383
A37 + Q169
R116 + Q125
L173 + S280
V238 + G255
Q345 + K391

Q32 + Q385
A37 + A186
R116 + G133
L173 + T285
V238 + Q256
Q345 + K393

Q32 + K391
A37 + E190
R116 + T134
L173 + M286
V238 + A263
Q345 + Q395

Q32 + K393
A37 + A225
R116 + W140
L173 + F289
V238 + V264
G346 + G348

Q32 + Q395
A37 + K242
R116 + G142
L173 + V291
V238 + A265
G346 + T355

A37 + T40
A37 + S244
R116 + G149
L173 + Q299
V238 + Y267
G346 + S376

A37 + P45
A37 + N260
R116 + T165
L173 + S304
V238 + N270
G346 + D377

A37 + W48
A37 + K269
R116 + W167
L173 + R320
V238 + G273
G346 + D379

A37 + G50
A37 + W284
R116 + R171
L173 + H321
V238 + S280
G346 + Y382

A37 + T51
A37 + Y295
R116 + Q172
L173 + S323
V238 + T285
G346 + S383

A37 + N54
A37 + V326
R116 + L173
L173 + H324
V238 + M286
G346 + Q385

A37 + V56
T40 + Q169
R116 + A174
L173 + F328
V238 + F289
G346 + K391

A37 + A60
T40 + A186
R116 + G184
L173 + T334
V238 + V291
G346 + K393

A37 + K72
T40 + E190
R116 + T193
L173 + D337
V238 + Q299
G346 + Q395

A37 + R87
T40 + A225
R116 + N195
L173 + Q345
V238 + S304
G348 + T355

A37 + Q98
T40 + K242
R116 + G196
L173 + G346
V238 + R320
G348 + S376

A37 + M105
T40 + S244
R116 + A204
L173 + G348
V238 + H321
G348 + D377

A37 + G109
T40 + N260
R116 + V206
L173 + T355
V238 + S323
G348 + D379

A37 + F113
T40 + K269
R116 + P211
L173 + S376
V238 + H324
G348 + Y382

A37 + R116
T40 + W284
R116 + I214
L173 + D377
V238 + F328
G348 + S383

A37 + Q118
T40 + Y295
R116 + V215
L173 + D379
V238 + T334
G348 + Q385

A37 + Q125
T40 + V326
R116 + L217
L173 + Y382
V238 + D337
G348 + K391

A37 + G133
P45 + Q169
R116 + L219
L173 + S383
V238 + Q345
G348 + K393

A37 + T134
P45 + A186
R116 + L228
L173 + Q385
V238 + G346
G348 + Q395

A37 + W140
P45 + E190
R116 + L235
L173 + K391
V238 + G348
T355 + S376

A37 + G142
P45 + A225
R116 + V238
L173 + K393
V238 + T355
T355 + D377

A37 + G149
P45 + K242
R116 + M246
L173 + Q395
V238 + S376
T355 + D379

A37 + T165
P45 + S244
R116 + M248
A174 + G184
V238 + D377
T355 + Y382

A37 + W167
P45 + N260
R116 + L250
A174 + T193
V238 + D379
T355 + S383

A37 + R171
P45 + K269
R116 + G255
A174 + N195
V238 + Y382
T355 + Q385

A37 + Q172
P45 + W284
R116 + Q256
A174 + G196
V238 + S383
T355 + K391

A37 + L173
P45 + Y295
R116 + A263
A174 + A204
V238 + Q385
T355 + K393

A37 + A174
P45 + V326
R116 + V264
A174 + V206
V238 + K391
T355 + Q395

A37 + G184
W48 + Q169
R116 + A265
A174 + P211
V238 + K393
S376 + D377

A37 + T193
W48 + A186
R116 + Y267
A174 + I214
V238 + Q395
S376 + D379

A37 + N195
W48 + E190
R116 + N270
A174 + V215
M246 + M248
S376 + Y382

A37 + G196
W48 + A225
R116 + G273
A174 + L217
M246 + L250
S376 + S383

A37 + A204
W48 + K242
R116 + S280
A174 + L219
M246 + G255
S376 + Q385

A37 + V206
W48 + S244
R116 + T285
A174 + L228
M246 + Q256
S376 + K391

A37 + P211
W48 + N260
R116 + M286
A174 + L235
M246 + A263
S376 + K393

A37 + I214
W48 + K269
R116 + F289
A174 + V238
M246 + V264
S376 + Q395

A37 + V215
W48 + W284
R116 + V291
A174 + M246
M246 + A265
D377 + D379

A37 + L217
W48 + Y295
R116 + Q299
A174 + M248
M246 + Y267
D377 + Y382

A37 + L219
W48 + V326
R116 + S304
A174 + L250
M246 + N270
D377 + S383

A37 + L228
G50 + Q169
R116 + R320
A174 + G255
M246 + G273
D377 + Q385

A37 + L235
G50 + A186
R116 + H321
A174 + Q256
M246 + S280
D377 + K391

A37 + V238
G50 + E190
R116 + S323
A174 + A263
M246 + T285
D377 + K393

A37 + M246
G50 + A225
R116 + H324
A174 + V264
M246 + M286
D377 + Q395

A37 + M248
G50 + K242
R116 + F328
A174 + A265
M246 + F289
D379 + Y382

A37 + L250
G50 + S244
R116 + T334
A174 + Y267
M246 + V291
D379 + S383

A37 + G255
G50 + N260
R116 + D337
A174 + N270
M246 + Q299
D379 + Q385

A37 + Q256
G50 + K269
R116 + Q345
A174 + G273
M246 + S304
D379 + K391

A37 + A263
G50 + W284
R116 + G346
A174 + S280
M246 + R320
D379 + K393

A37 + V264
G50 + Y295
R116 + G348
A174 + T285
M246 + H321
D379 + Q395

A37 + A265
G50 + V326
R116 + T355
A174 + M286
M246 + S323
Y382 + S383

A37 + Y267
T51 + Q169
R116 + S376
A174 + F289
M246 + H324
Y382 + Q385

A37 + N270
T51 + A186
R116 + D377
A174 + V291
M246 + F328
Y382 + K391

A37 + G273
T51 + E190
R116 + D379
A174 + Q299
M246 + T334
Y382 + K393

A37 + S280
T51 + A225
R116 + Y382
A174 + S304
M246 + D337
Y382 + Q395

A37 + T285
T51 + K242
R116 + S383
A174 + R320
M246 + Q345
S383 + Q385

A37 + M286
T51 + S244
R116 + Q385
A174 + H321
M246 + G346
S383 + K391

A37 + F289
T51 + N260
R116 + K391
A174 + S323
M246 + G348
S383 + K393

A37 + V291
T51 + K269
R116 + K393
A174 + H324
M246 + T355
S383 + Q395

A37 + Q299
T51 + W284
R116 + Q395
A174 + F328
M246 + S376
Q385 + K391

A37 + S304
T51 + Y295
Q118 + Q125
A174 + T334
M246 + D377
Q385 + K393

A37 + R320
T51 + V326
Q118 + G133
A174 + D337
M246 + D379
Q385 + Q395

A37 + H321
N54 + Q169
Q118 + T134
A174 + Q345
M246 + Y382
K391 + K393

A37 + S323
N54 + A186
Q118 + W140
A174 + G346
M246 + S383
K391 + Q395

A37 + H324
N54 + E190
Q118 + G142
A174 + G348
M246 + Q385
K393 + Q395

A37 + F328
N54 + A225
Q118 + G149
A174 + T355
M246 + K391
M248 + F289

A37 + T334
N54 + K242
Q118 + T165
A174 + S376
M246 + K393
M248 + V291

A37 + D337
N54 + S244
Q118 + W167
A174 + D377
M246 + Q395
G109 + Q169

A37 + Q345
N54 + N260
Q118 + R171
A174 + D379
M248 + L250
G109 + A186

A37 + G346
N54 + K269
Q118 + Q172
A174 + Y382
M248 + G255
G109 + E190

A37 + G348
N54 + W284
Q118 + L173
A174 + S383
M248 + Q256
G109 + A225

A37 + T355
N54 + Y295
Q118 + A174
A174 + Q385
M248 + A263
G109 + K242

A37 + S376
N54 + V326
Q118 + G184
A174 + K391
M248 + V264
G109 + S244

A37 + D377
V56 + Q169
Q118 + T193
A174 + K393
M248 + A265
G109 + N260

A37 + D379
V56 + A186
Q118 + N195
A174 + Q395
M248 + Y267
G109 + K269

A37 + Y382
V56 + E190
Q118 + G196
G184 + T193
M248 + N270
G109 + W284

A37 + S383
V56 + A225
Q118 + A204
G184 + N195
M248 + G273
G109 + Y295

A37 + Q385
V56 + K242
Q118 + V206
G184 + G196
M248 + S280
G109 + V326

A37 + K391
V56 + S244
Q118 + P211
G184 + A204
M248 + T285
F113 + Q169

A37 + K393
V56 + N260
Q118 + I214
G184 + V206
M248 + M286
F113 + A186

A60 + Q169
V56 + K269
G133 + Q169
W167 + Q169
R171 + A186
F113 + A225

A60 + A186
V56 + W284
G133 + A186
W167 + A186
R171 + E190
F113 + E190

A60 + E190
V56 + Y295
G133 + E190
W167 + E190
R171 + A225
F113 + K242

A60 + A225
V56 + V326
G133 + A225
W167 + A225
R171 + K242
F113 + S244

A60 + K242
G184 + A186
G133 + K242
W167 + K242
R171 + S244
F113 + N260

A60 + S244
G184 + E190
G133 + S244
W167 + S244
R171 + N260
F113 + K269

A60 + N260
G184 + A225
G133 + N260
W167 + N260
R171 + K269
F113 + W284

A60 + K269
G184 + K242
G133 + K269
W167 + K269
R171 + W284
F113 + Y295

A60 + W284
G184 + S244
G133 + W284
W167 + W284
R171 + Y295
F113 + V326

A60 + Y295
G184 + N260
G133 + Y295
W167 + Y295
R171 + V326
R116 + Q169

A60 + V326
G184 + K269
G133 + V326
W167 + V326
Q172 + A186
R116 + A186

K72 + Q169
G184 + W284
T134 + Q169
Q169 + A186
Q172 + E190
R116 + E190

K72 + A186
G184 + Y295
T134 + A186
Q169 + E190
Q172 + A225
R116 + A225

K72 + E190
G184 + V326
T134 + E190
Q169 + L173
Q172 + K242
R116 + K242

K72 + A225
A186 + E190
T134 + A225
Q169 + A174
Q172 + S244
R116 + S244

K72 + K242
A186 + T193
T134 + K242
Q169 + G184
Q172 + N260
R116 + N260

K72 + S244
A186 + N195
T134 + S244
Q169 + T193
Q172 + K269
R116 + K269

K72 + N260
A186 + G196
T134 + N260
Q169 + N195
Q172 + W284
R116 + W284

K72 + K269
A186 + A204
T134 + K269
Q169 + G196
Q172 + Y295
R116 + Y295

K72 + W284
A186 + V206
T134 + W284
Q169 + A204
Q172 + V326
R116 + V326

K72 + Y295
A186 + P211
T134 + Y295
Q169 + V206
L173 + A186
Q118 + Q169

K72 + V326
A186 + I214
T134 + V326
Q169 + P211
L173 + E190
Q118 + A186

R87 + Q169
A186 + V215
W140 + Q169
Q169 + I214
L173 + A225
Q118 + A225

R87 + A186
A186 + L217
W140 + A186
Q169 + V215
L173 + K242
Q118 + K242

R87 + E190
A186 + L219
W140 + E190
Q169 + L217
L173 + S244
Q118 + S244

R87 + A225
A186 + A225
W140 + A225
Q169 + L219
L173 + N260
Q118 + N260

R87 + K242
A186 + L228
W140 + K242
Q169 + A225
L173 + K269
Q118 + K269

R87 + S244
A186 + L235
W140 + S244
Q169 + L228
L173 + W284
Q118 + W284

R87 + N260
A186 + V238
W140 + N260
Q169 + L235
L173 + Y295
Q118 + Y295

R87 + K269
A186 + K242
W140 + K269
Q169 + V238
L173 + V326
Q118 + V326

R87 + W284
A186 + S244
W140 + W284
Q169 + K242
A174 + A186
Q125 + Q169

R87 + Y295
A186 + M246
W140 + Y295
Q169 + S244
A174 + E190
Q125 + A186

R87 + V326
A186 + M248
W140 + V326
Q169 + M246
A174 + A225
Q125 + E190

Q98 + Q169
A186 + L250
G142 + Q169
Q169 + M248
A174 + K242
Q125 + A225

Q98 + A186
A186 + G255
G142 + A186
Q169 + L250
A174 + S244
Q125 + K242

Q98 + E190
A186 + Q256
G142 + E190
Q169 + G255
A174 + N260
Q125 + S244

Q98 + A225
A186 + N260
G142 + A225
Q169 + Q256
A174 + K269
Q125 + N260

Q98 + K242
A186 + A263
G142 + K242
Q169 + N260
A174 + W284
Q125 + K269

Q98 + S244
A186 + V264
G142 + S244
Q169 + A263
A174 + Y295
Q125 + W284

Q98 + N260
A186 + A265
G142 + N260
Q169 + V264
A174 + V326
Q125 + Y295

Q98 + K269
A186 + Y267
G142 + K269
Q169 + A265
T193 + A225
Q125 + V326

Q98 + W284
A186 + K269
G142 + W284
Q169 + Y267
T193 + K242
V215 + A225

Q98 + Y295
A186 + N270
G142 + Y295
Q169 + K269
T193 + S244
V215 + K242

Q98 + V326
A186 + G273
G142 + V326
Q169 + N270
T193 + N260
V215 + S244

M105 + Q169
A186 + S280
G149 + Q169
Q169 + G273
T193 + K269
V215 + N260

M105 + A186
A186 + W284
G149 + A186
Q169 + S280
T193 + W284
V215 + K269

M105 + E190
A186 + T285
G149 + E190
Q169 + W284
T193 + Y295
V215 + W284

M105 + A225
A186 + M286
G149 + A225
Q169 + T285
T193 + V326
V215 + Y295

M105 + K242
A186 + F289
G149 + K242
Q169 + M286
N195 + A225
V215 + V326

M105 + S244
A186 + V291
G149 + S244
Q169 + F289
N195 + K242
L217 + A225

M105 + N260
A186 + Y295
G149 + N260
Q169 + V291
N195 + S244
L217 + K242

M105 + K269
A186 + Q299
G149 + K269
Q169 + Y295
N195 + N260
L217 + S244

M105 + W284
A186 + S304
G149 + W284
Q169 + Q299
N195 + K269
L217 + N260

M105 + Y295
A186 + R320
G149 + Y295
Q169 + S304
N195 + W284
L217 + K269

M105 + V326
A186 + H321
G149 + V326
Q169 + R320
N195 + Y295
L217 + W284

A225 + L228
A186 + S323
T165 + Q169
Q169 + H321
N195 + V326
L217 + Y295

A225 + L235
A186 + H324
T165 + A186
Q169 + S323
G196 + A225
L217 + V326

A225 + V238
A186 + V326
T165 + E190
Q169 + H324
G196 + K242
L219 + A225

A225 + K242
A186 + F328
T165 + A225
Q169 + V326
G196 + K242
L219 + K242

A225 + S244
A186 + T334
T165 + K242
Q169 + F328
G196 + S244
L219 + S244

A225 + M246
A186 + D337
T165 + S244
Q169 + T334
G196 + N260
L219 + N260

A225 + M248
A186 + Q345
T165 + N260
Q169 + D337
G196 + K269
L219 + K269

A225 + L250
A186 + G346
T165 + K269
Q169 + Q345
G196 + W284
L219 + W284

A225 + G255
A186 + G348
T165 + W284
Q169 + G346
G196 + Y295
L219 + Y295

A225 + Q256
A186 + T355
T165 + Y295
Q169 + G348
G196 + V326
L219 + V326

A225 + N260
A186 + S376
T165 + V326
Q169 + T355
A204 + A225
M246 + N260

A225 + A263
A186 + D377
L228 + K242
Q169 + S376
A204 + K242
M246 + K269

A225 + V264
A186 + D379
L228 + S244
Q169 + D377
A204 + S244
M246 + W284

A225 + A265
A186 + Y382
L228 + N260
Q169 + D379
A204 + N260
M246 + Y295

A225 + Y267
A186 + S383
L228 + K269
Q169 + Y382
A204 + K269
M246 + V326

A225 + K269
A186 + Q385
L228 + W284
Q169 + S383
A204 + W284
M248 + N260

A225 + N270
A186 + K391
L228 + Y295
Q169 + Q385
A204 + Y295
M248 + K269

A225 + G273
A186 + K393
L228 + V326
Q169 + K391
A204 + V326
M248 + W284

A225 + S280
A186 + Q395
L235 + K242
Q169 + K393
V206 + A225
M248 + Y295

A225 + W284
E190 + T193
L235 + S244
Q169 + Q395
A206 + K242
M248 + V326

A225 + T285
E190 + N195
L235 + N260
Q256 + N260
A206 + S244
L250 + N260

A225 + M286
E190 + G196
L235 + K269
G256 + K269
A206 + N260
L250 + K269

A225 + F289
E190 + A204
L235 + W284
Q256 + W284
A206 + K269
L250 + W284

A225 + V291
E190 + V206
L235 + Y295
Q256 + Y295
A206 + W284
L250 + Y295

A225 + Y295
E190 + P211
L235 + V326
Q256 + V326
A206 + Y295
L250 + V326

A225 + Q299
E190 + I214
V238 + K242
N260 + A263
A206 + V326
G255 + N260

A225 + S304
E190 + V215
V238 + S244
N260 + V264
P211 + A225
G255 + K269

A225 + R320
E190 + L217
V238 + N260
N260 + A265
P211 + K242
G255 + W284

A225 + H321
E190 + L219
V238 + K269
N260 + Y267
P211 + S244
G255 + Y295

A225 + S323
E190 + A225
V238 + W284
N260 + K269
P211 + N206
G255 + V326

A225 + H324
E190 + L228
V238 + Y295
N260 + N270
P211 + K269
N270 + W284

A225 + V326
E190 + L235
V238 + V326
N260 + G273
P211 + W284
N270 + Y295

A225 + F328
E190 + V238
K242 + S244
N260 + S280
P211 + Y295
N270 + V326

A225 + T334
E190 + K242
K242 + M246
N260 + W284
P211 + V326
G273 + W284

A225 + D337
E190 + S244
K242 + M248
N260 + T285
I214 + A225
G273 + Y295

A225 + Q345
E190 + M246
K242 + L250
N260 + M286
I214 + K242
G273 + V326

A225 + G346
E190 + M248
K242 + G255
N260 + F289
I214 + S244
S280 + W284

A225 + G348
E190 + L250
K242 + Q256
N260 + V291
I214 + N260
S280 + Y295

A225 + T355
E190 + G255
K242 + N260
N260 + Y295
I214 + K269
S280 + V326

A225 + S376
E190 + Q256
K242 + A263
N260 + Q299
I214 + W284
W284 + T285

A225 + D377
E190 + N260
K242 + V264
N260 + S304
I214 + Y295
W284 + M286

A225 + D379
E190 + A263
K242 + A265
N260 + R320
I214 + V326
W284 + F289

A225 + Y382
E190 + V264
K242 + Y267
N260 + H321
K269 + N270
W284 + V291

A225 + S383
E190 + A265
K242 + K269
N260 + S323
K269 + G273
W284 + Y295

A225 + Q385
E190 + Y267
K242 + N270
N260 + H324
K269 + S280
W284 + Q299

A225 + K391
E190 + K269
K242 + G273
N260 + V326
K269 + W284
W284 + S304

A225 + K393
E190 + N270
K242 + S280
N260 + F328
K269 + T285
W284 + R320

A225 + Q395
E190 + G273
K242 + W284
N260 + T334
K269 + M286
W284 + H321

A263 + K269
E190 + S280
K242 + T285
N260 + D337
K269 + F289
W284 + S323

A263 + W284
E190 + W284
K242 + M286
N260 + Q345
K269 + V291
W284 + H324

A263 + Y295
E190 + T285
K242 + F289
N260 + G346
K269 + Y295
W284 + V326

A263 + V326
E190 + M286
K242 + V291
N260 + G348
K269 + Q299
W284 + F328

V264 + K269
E190 + F289
K242 + Y295
N260 + T355
K269 + S304
W284 + T334

V264 + W284
E190 + V291
K242 + Q299
N260 + S376
K269 + R320
W284 + D337

V264 + Y295
E190 + Y295
K242 + S304
N260 + D377
K269 + H321
W284 + Q345

V264 + V326
E190 + Q299
K242 + R320
N260 + D379
K269 + S323
W284 + G346

A265 + K269
E190 + S304
K242 + H321
N260 + Y382
K269 + H324
W284 + G348

A265 + W284
E190 + R320
K242 + S323
N260 + S383
K269 + V326
W284 + T355

A265 + Y295
E190 + H321
K242 + H324
N260 + Q385
K269 + F328
W284 + S376

A265 + V326
E190 + S323
K242 + V326
N260 + K391
K269 + T334
W284 + D377

A265 + K269
E190 + H324
K242 + F328
N260 + K393
K269 + D337
W284 + D379

A265 + W284
E190 + V326
K242 + T334
N260 + Q395
K269 + Q345
W284 + Y382

A265 + Y295
E190 + F328
K242 + D337
Y295 + S376
K269 + G346
W284 + S383

A265 + V326
E190 + T334
K242 + Q345
V326 + S376
K269 + G348
W284 + Q385

Y295 + D377
E190 + D337
K242 + G346
V326 + D377
K269 + T355
W284 + K391

Y295 + D379
E190 + Q345
K242 + G348
V326 + D379
K269 + S376
W284 + K393

Y295 + Y382
E190 + G346
K242 + T355
V326 + Y382
K269 + D377
W284 + Q395

Y295 + S383
E190 + G348
K242 + S376
V326 + S383
K269 + D379
T285 + Y295

Y295 + Q385
E190 + T355
K242 + D377
V326 + Q385
K269 + Y382
T285 + V326

Y295 + K391
E190 + S376
K242 + D379
V326 + K391
K269 + S383
M286 + Y295

Y295 + K393
E190 + D377
K242 + Y382
V326 + K393
K269 + Q385
M286 + V326

Y295 + Q395
E190 + D379
K242 + S383
V326 + Q395
K269 + K391
F289 + Y295

Q299 + V326
E190 + Y382
K242 + Q385
S244 + K393
K269 + K393
F289 + V326

S304 + V326
E190 + S383
K242 + K391
S244 + Q395
K269 + Q395
V291 + Y295

R320 + V326
E190 + Q385
K242 + K393
S244 + Y267
S244 + R320
V294 + V326

H321 + V326
E190 + K391
K242 + Q395
S244 + K269
S244 + H321
Y295 + Q299

S323 + V326
E190 + K393
S244 + M246
S244 + N270
S244 + S323
Y295 + S304

H324 + V326
E190 + Q395
S244 + M248
S244 + G273
S244 + H324
Y295 + R320

V326 + F328
S244 + F289
S244 + L250
S244 + S280
S244 + V326
Y295 + H321

V326 + T334
S244 + V291
S244 + G255
S244 + W284
S244 + F328
Y295 + S323

V326 + D337
S244 + Y295
S244 + Q256
S244 + T285
S244 + T334
Y295 + H324

V326 + Q345
S244 + Q299
S244 + N260
S244 + S376
S244 + D337
Y295 + V326

V326 + G346
S244 + S304
S244 + A263
S244 + D377
S244 + Q345
Y295 + F328

V326 + G348
S244 + K391
S244 + V264
S244 + D379
S244 + G346
Y295 + T334

V326 + T355
S244 + M286
S244 + A265
S244 + Y382
S244 + G348
Y295 + F328

Y295 + G348
Y295 + Q345

S244 + S383
S244 + T355
Y295 + T334

Y295 + T355
Y295 + G346

S244 + Q385

Y295 + D337

In one particular embodiment, the modifications in one or more positions correspond to the following; H1*, T5K, G7K, G7A, Q11H, N16S, N16H, V17L, Q32S, A37H, A37M, A37V, A37S, A37Y, A37R, A37L, T40D, T40G, T40K, P45A, W48G, W48Y, W48F, G50A, T51K, T51E, T51G, T51A, T51S, T51G, T51D, N54S, V56T, A60V,K72R, K72H, K72S, K72Q, K72E, K72N, K72A, K72M, R87S, Q98S, Q98A, M105F, M105I, M105V, M105L, G109A, G109S, F113W, F113S, F113N, F113Y, F113R, F113L, F113Q, R116Q, R116V, R116K, R116W, R116L, R116A, R116H, R116M, R116E, R116S, R116I, R116G, Q118N, Q118K, Q118G, Q118S, Q118F, Q118R, Q125P, Q125K, Q125A, Q125T, N125D, G133S, F133Q, T134E, W140Y, G142T, G149Q, T165S, T165G, T165V, W167I, W167G, W167F, W167R, W167S, W167H, W167L, W167M, W167Y, Q169E, R171H, Q172G, Q172R, Q172N, Q172D, Q172Y, Q172M, Q172S, Q172T, Q172K, Q172H, Q172E, L173V, L173G, L173H, L173A, L173I, L173P, L173T, L173F, L173M, A174S, A174D, A174P, A174M, A174T, A174H, A174K, A174G, A174Q, A174N, A174V, A174L, G184T, A186D, A186N, A186E, A186Q, A186H, E190P, T193K, N195F, G196R, A204T, A204V, A204S, A204G, V206L, V206S, V206Y, P211D, I214G, I214H, I214S, I214T, I214L, I214E, I214W, V215T, L217T, L217Q, L219V, L219H, A225V, L228I, L235V, L235A, V238A, V238T, V238G, K242Q, S244Q, M246L, M246A, M246I, M246F, M246V, M246S, M248T, L250I, L250V, L250T, L250A, L250F, L250M, G255N, G255A, G255A, Q256A, N260G, A263G, V264I, V264T, A265G, Y2671, Y267M, Y267H, Y267L, K269Q, N270G, N270T, G273R, S280W, S280L, S280T, S280A, S280K, S280Q, W284H, T285L, T285Q, M286F, M286L, A288L, A288V, F289I, F289L, V291G, V291A, V291T, Y295N, Y295F, Q299V, S304N, S304R, R320A, R320V, H321Y, S323N, H324L, V326L, F328L, F328M, F328V, F328I, T334S, D337H, Q345D, G346T, G346D, G346P, G348S, G348P, T355L, T355F, S376H, S376T, S376V, D377H, D377S, D377A, D377Q, D379S, D379G, D379R, D379A, Y382M, Y382I, Y382L, Y382F, S383G, S383A, Q385L, K391A, K391Y, K391V, K391M, K391E, K391D, K391R, K391H, K391W, K391I, K391Q, K391L, K393Y, K393R, K393Q, K393S, and Q395P, wherein said positions correspond to positions of SEQ ID NOs: 7 or 10.

The inventors of the present invention have identified that these specific alterations within the A and B domain (as defined by SEQ ID NO: 10) of the amino acid sequence as set forth in SEQ ID NOs: 7 and 10, are particularly relevant for improving the performance of a variant alpha-amylase having at least 65% sequence identity to the parent polypeptide.

In one embodiment, the variant comprises an alteration in two, three, four, five, six, seven, eight, nine, ten, eleven, twelf, thirteen, fourteen, fifteen, sixteen, or seventeen positions corresponding to the following positions; H1*, T5K, G7K, G7A, Q11H, N16S, N16H, V17L, Q32S, A37H, A37M, A37V, A37S, A37Y, A37R, A37L, T40D, T40G, T40K, P45A, W48G, W48Y, W48F, G50A, T51K, T51E, T51G, T51A, T51S, T51G, T51D, N54S, V56T, A60V,K72R, K72H, K72S, K72Q, K72E, K72N, K72A, K72M, R87S, Q98S, Q98A, M105F, M105I, M105V, M105L, G109A, G109S, F113W, F113S, F113N, F113Y, F113R, F113L, F113Q, R116Q, R116V, R116K, R116W, R116L, R116A, R116H, R116M, R116E, R116S, R116I, R116G, Q118N, Q118K, Q118G, Q118S, Q118F, Q118R, Q125P, Q125K, Q125A, Q125T, N125D, G133S, F133Q, T134E, W140Y, G142T, G149Q, T165S, T165G, T165V, W167I, W167G, W167F, W167R, W167S, W167H, W167L, W167M, W167Y, Q169E, R171H, Q172G, Q172R, Q172N, Q172D, Q172Y, Q172M, Q172S, Q172T, Q172K, Q172H, Q172E, L173V, L173G, L173H, L173A, L173I, L173P, L173T, L173F, L173M, A174S, A174D, A174P, A174M, A174T, A174H, A174K, A174G, A174Q, A174N, A174V, A174L, G184T, A186D, A186N, A186E, A186Q, A186H, E190P, T193K, N195F, G196R, A204T, A204V, A204S, A204G, V206L, V206S, V206Y, P211D, I214G, I214H, I214S, I214T, I214L, I214E, I214W, V215T, L217T, L217Q, L219V, L219H, A225V, L228I, L235V, L235A, V238A, V238T, V238G, K242Q, S244Q, M246L, M246A, M246I, M246F, M246V, M246S, M248T, L250I, L250V, L250T, L250A, L250F, L250M, G255N, G255A, G255S, Q256A, N260G, A263G, V264I, V264T, A265G, Y267I, Y267M, Y267H, Y267L, K269Q, N270G, N270T, G273R, S280W, S280L, S280T, S280A, S280K, S280Q, W284H, T285L, T285Q, M286F, M286L, A288L, A288V, F289I, F289L, V291G, V291A, V291T, Y295N, Y295F, Q299V, S304N, S304R, R320A, R320V, H321Y, S323N, H324L, V326L, F328L, F328M, F328V, F328I, T334S, D337H, Q345D, G346T, G346D, G346P, G348S, G348P, T355L, T355F, S376H, S376T, S376V, D377H, D377S, D377A, D377Q, D379S, D379G, D379R, D379A, Y382M, Y382I, Y382L, Y382F, S383G, S383A, Q385L, K391A, K391Y, K391V, K391M, K391E, K391D, K391R, K391H, K391W, K391I, K391Q, K391L, K393Y, K393R, K393Q, K393S, and Q395P, wherein said positions correspond to positions of SEQ ID NOs: 7 or 10.

The C domain of a variant according to the present invention may also comprise alterations. Thus, in one embodiment, the variant comprises an alteration in one or more amino acid positions corresponding to the amino acid positions of the amino acid sequence set forth in SEQ ID NOs: 11 or 12, or an amino acid sequence having at least 65% sequence identity to an amino acid sequence as set forth in SEQ ID NOs: 11 or 12.

In one particular embodiment, the C domain comprises an alteration in one or more of the following positions corresponding to positions; A420, G423, T444, A445, Q449, T459, P473, C474, G476, G477, and K484 of SEQ ID NO: 13 or I405, A421, A422, A428, R439 G448, W467, D476, and G477 of SEQ ID NO: 14.

In one embodiment, the variant comprises an alteration in two, three, four, five, six, seven, or eight positions corresponding to the following positions; A420, G423, T444, A445, Q449, T459, P473, C474, G476, G477, and K484 of SEQ ID NO: 13 or I405, A421, A422, A428, R439 G448, W467, D476, and G477 of SEQ ID NO: 14.

The inventors of the present invention have identified that specific amino acid substitutions within the C domain of parent polypeptide have an improved effect on performance of the variants. Thus, in a particular embodiment, the alteration in one or more positions correspond to the following T400P, H402R, A420Q, A420S, A420K, A420L, G423H, T444Q, T444S, T444D, T444Y, T444H, T444V, T444R, T444A, A445Q, Q449T, T459N, P473R, P473A, P473G, P473T, P473K, C474V, G476K, G477A, G477Q, K484A, K484G, K484P, K484E, K484Q, and K484S, wherein said positions correspond to positions of SEQ ID NO: 13; or I405L, A421H, A422P, A428T, G448D, D476K, D476G, D476N, D476Y, G477S, G477A, G477T, and G477Q, wherein said positions correspond to positions of SEQ ID NO: 14.

In a particular embodiment, the variant comprises a modification in the following positions;

H1 + T5
T40 + A174
R87 + N270
W140 + K393
V206 + V215
Y267 + C474

H1 + G7
T40 + G184
R87 + G273
W140 + Q395
V206 + L217
Y267 + G476

H1 + Q11
T40 + T193
R87 + S280
W140 + A420
V206 + L219
Y267 + G477

H1 + N16
T40 + N195
R87 + T285
W140 + G423
V206 + L235
Y267 + K484

H1 + V17
T40 + A204
R87 + M286
W140 + T444
V206 + V238
N270 + G273

H1 + Q32
T40 + V206
R87 + F289
W140 + A445
V206 + M246
N270 + S280

H1 + A37
T40 + P211
R87 + V291
W140 + Q449
V206 + M248
N270 + T285

H1 + T40
T40 + I214
R87 + Q299
W140 + T459
V206 + L250
N270 + M286

H1 + P45
T40 + V215
R87 + R320
W140 + P473
V206 + G255
N270 + F289

H1 + W48
T40 + L217
R87 + H321
W140 + C474
V206 + Q256
N270 + V291

H1 + G50
T40 + L219
R87 + S323
W140 + G476
V206 + A263
N270 + Q299

H1 + T51
T40 + L235
R87 + H324
W140 + G477
V206 + V264
N270 + R320

H1 + N54
T40 + V238
R87 + F328
W140 + K484
V206 + Y267
N270 + H321

H1 + V56
T40 + M246
R87 + T334
G142 + G149
V206 + N270
N270 + S323

H1 + K72
T40 + M248
R87 + D337
G142 + T165
V206 + G273
N270 + H324

H1 + R87
T40 + L250
R87 + Q345
G142 + W167
V206 + S280
N270 + F328

H1 + Q98
T40 + G255
R87 + G346
G142 + R171
V206 + T285
N270 + T334

H1 + M105
T40 + Q256
R87 + G348
G142 + Q172
V206 + M286
N270 + D337

H1 + G109
T40 + A263
R87 + T355
G142 + L173
V206 + F289
N270 + Q345

H1 + F113
T40 + V264
R87 + S376
G142 + A174
V206 + V291
N270 + G346

H1 + R116
T40 + Y267
R87 + D377
G142 + G184
V206 + Q299
N270 + G348

H1 + Q118
T40 + N270
R87 + D379
G142 + T193
V206 + R320
N270 + T355

H1 + Q125
T40 + G273
R87 + Y382
G142 + N195
V206 + H321
N270 + S376

H1 + G133
T40 + S280
R87 + S383
G142 + A204
V206 + S323
N270 + D377

H1 + T134
T40 + T285
R87 + Q385
G142 + V206
V206 + H324
N270 + D379

H1 + W140
T40 + M286
R87 + K391
G142 + P211
V206 + F328
N270 + Y382

H1 + G142
T40 + F289
R87 + K393
G142 + I214
V206 + T334
N270 + S383

H1 + G149
T40 + V291
R87 + Q395
G142 + V215
V206 + D337
N270 + Q385

H1 + T165
T40 + Q299
R87 + A420
G142 + L217
V206 + Q345
N270 + K391

H1 + W167
T40 + R320
R87 + G423
G142 + L219
V206 + G346
N270 + K393

H1 + R171
T40 + H321
R87 + T444
G142 + L235
V206 + G348
N270 + Q395

H1 + Q172
T40 + S323
R87 + A445
G142 + V238
V206 + T355
N270 + A420

H1 + L173
T40 + H324
R87 + Q449
G142 + M246
V206 + S376
N270 + G423

H1 + A174
T40 + F328
R87 + T459
G142 + M248
V206 + D377
N270 + T444

H1 + G184
T40 + T334
R87 + P473
G142 + L250
V206 + D379
N270 + A445

H1 + T193
T40 + D337
R87 + C474
G142 + G255
V206 + Y382
N270 + Q449

H1 + N195
T40 + Q345
R87 + G476
G142 + Q256
V206 + S383
N270 + T459

H1 + A204
T40 + G346
R87 + G477
G142 + A263
V206 + Q385
N270 + P473

H1 + V206
T40 + G348
R87 + K484
G142 + V264
V206 + K391
N270 + C474

H1 + P211
T40 + T355
Q98 + M105
G142 + Y267
V206 + K393
N270 + G476

H1 + I214
T40 + S376
Q98 + G109
G142 + N270
V206 + Q395
N270 + G477

H1 + V215
T40 + D377
Q98 + F113
G142 + G273
V206 + A420
N270 + K484

H1 + L217
T40 + D379
Q98 + R116
G142 + S280
V206 + G423
G273 + S280

H1 + L219
T40 + Y382
Q98 + Q118
G142 + T285
V206 + T444
G273 + T285

H1 + L235
T40 + S383
Q98 + Q125
G142 + M286
V206 + A445
G273 + M286

H1 + V238
T40 + Q385
Q98 + G133
G142 + F289
V206 + Q449
G273 + F289

H1 + M246
T40 + K391
Q98 + T134
G142 + V291
V206 + T459
G273 + V291

H1 + M248
T40 + K393
Q98 + W140
G142 + Q299
V206 + P473
G273 + Q299

H1 + L250
T40 + Q395
Q98 + G142
G142 + R320
V206 + C474
G273 + R320

H1 + G255
T40 + A420
Q98 + G149
G142 + H321
V206 + G476
G273 + H321

H1 + Q256
T40 + G423
Q98 + T165
G142 + S323
V206 + G477
G273 + S323

H1 + A263
T40 + T444
Q98 + W167
G142 + H324
V206 + K484
G273 + H324

H1 + V264
T40 + A445
Q98 + R171
G142 + F328
P211 + I214
G273 + F328

H1 + Y267
T40 + Q449
Q98 + Q172
G142 + T334
P211 + V215
G273 + T334

H1 + N270
T40 + T459
Q98 + L173
G142 + D337
P211 + L217
G273 + D337

H1 + G273
T40 + P473
Q98 + A174
G142 + Q345
P211 + L219
G273 + Q345

H1 + S280
T40 + C474
Q98 + G184
G142 + G346
P211 + L235
G273 + G346

H1 + T285
T40 + G476
Q98 + T193
G142 + G348
P211 + V238
G273 + G348

H1 + M286
T40 + G477
Q98 + N195
G142 + T355
P211 + M246
G273 + T355

H1 + F289
T40 + K484
Q98 + A204
G142 + S376
P211 + M248
G273 + S376

H1 + V291
P45 + W48
Q98 + V206
G142 + D377
P211 + L250
G273 + D377

H1 + Q299
P45 + G50
Q98 + P211
G142 + D379
P211 + G255
G273 + D379

H1 + R320
P45 + T51
Q98 + I214
G142 + Y382
P211 + Q256
G273 + Y382

H1 + H321
P45 + N54
Q98 + V215
G142 + S383
P211 + A263
G273 + S383

H1 + S323
P45 + V56
Q98 + L217
G142 + Q385
P211 + V264
G273 + Q385

H1 + H324
P45 + K72
Q98 + L219
G142 + K391
P211 + Y267
G273 + K391

H1 + F328
P45 + R87
Q98 + L235
G142 + K393
P211 + N270
G273 + K393

H1 + T334
P45 + Q98
Q98 + V238
G142 + Q395
P211 + G273
G273 + Q395

H1 + D337
P45 + M105
Q98 + M246
G142 + A420
P211 + S280
G273 + A420

H1 + Q345
P45 + G109
Q98 + M248
G142 + G423
P211 + T285
G273 + G423

H1 + G346
P45 + F113
Q98 + L250
G142 + T444
P211 + M286
G273 + T444

H1 + G348
P45 + R116
Q98 + G255
G142 + A445
P211 + F289
G273 + A445

H1 + T355
P45 + Q118
Q98 + Q256
G142 + Q449
P211 + V291
G273 + Q449

H1 + S376
P45 + Q125
Q98 + A263
G142 + T459
P211 + Q299
G273 + T459

H1 + D377
P45 + G133
Q98 + V264
G142 + P473
P211 + R320
G273 + P473

H1 + D379
P45 + T134
Q98 + Y267
G142 + C474
P211 + H321
G273 + C474

H1 + Y382
P45 + W140
Q98 + N270
G142 + G476
P211 + S323
G273 + G476

H1 + S383
P45 + G142
Q98 + G273
G142 + G477
P211 + H324
G273 + G477

H1 + Q385
P45 + G149
Q98 + S280
G142 + K484
P211 + F328
G273 + K484

H1 + K391
P45 + T165
Q98 + T285
G149 + T165
P211 + T334
S280 + T285

H1 + K393
P45 + W167
Q98 + M286
G149 + W167
P211 + D337
S280 + M286

H1 + Q395
P45 + R171
Q98 + F289
G149 + R171
P211 + Q345
S280 + F289

H1 + A420
P45 + Q172
Q98 + V291
G149 + Q172
P211 + G346
S280 + V291

H1 + G423
P45 + L173
Q98 + Q299
G149 + L173
P211 + G348
S280 + Q299

H1 + T444
P45 + A174
Q98 + R320
G149 + A174
P211 + T355
S280 + R320

H1 + A445
P45 + G184
Q98 + H321
G149 + G184
P211 + S376
S280 + H321

H1 + Q449
P45 + T193
Q98 + S323
G149 + T193
P211 + D377
S280 + S323

H1 + T459
P45 + N195
Q98 + H324
G149 + N195
P211 + D379
S280 + H324

H1 + P473
P45 + A204
Q98 + F328
G149 + A204
P211 + Y382
S280 + F328

H1 + C474
P45 + V206
Q98 + T334
G149 + V206
P211 + S383
S280 + T334

H1 + G476
P45 + P211
Q98 + D337
G149 + P211
P211 + Q385
S280 + D337

H1 + G477
P45 + I214
Q98 + Q345
G149 + I214
P211 + K391
S280 + Q345

H1 + K484
P45 + V215
Q98 + G346
G149 + V215
P211 + K393
S280 + G346

T5 + G7
P45 + L217
Q98 + G348
G149 + L217
P211 + Q395
S280 + G348

T5 + Q11
P45 + L219
Q98 + T355
G149 + L219
P211 + A420
S280 + T355

T5 + N16
P45 + L235
Q98 + S376
G149 + L235
P211 + G423
S280 + S376

T5 + V17
P45 + V238
Q98 + D377
G149 + V238
P211 + T444
S280 + D377

T5 + Q32
P45 + M246
Q98 + D379
G149 + M246
P211 + A445
S280 + D379

T5 + A37
P45 + M248
Q98 + Y382
G149 + M248
P211 + Q449
S280 + Y382

T5 + T40
P45 + L250
Q98 + S383
G149 + L250
P211 + T459
S280 + S383

T5 + P45
P45 + G255
Q98 + Q385
G149 + G255
P211 + P473
S280 + Q385

T5 + W48
P45 + Q256
Q98 + K391
G149 + Q256
P211 + C474
S280 + K391

T5 + G50
P45 + A263
Q98 + K393
G149 + A263
P211 + G476
S280 + K393

T5 + T51
P45 + V264
Q98 + Q395
G149 + V264
P211 + G477
S280 + Q395

T5 + N54
P45 + Y267
Q98 + A420
G149 + Y267
P211 + K484
S280 + A420

T5 + V56
P45 + N270
Q98 + G423
G149 + N270
I214 + V215
S280 + G423

T5 + K72
P45 + G273
Q98 + T444
G149 + G273
I214 + L217
S280 + T444

T5 + R87
P45 + S280
Q98 + A445
G149 + S280
I214 + L219
S280 + A445

T5 + Q98
P45 + T285
Q98 + Q449
G149 + T285
I214 + L235
S280 + Q449

T5 + M105
P45 + M286
Q98 + T459
G149 + M286
I214 + V238
S280 + T459

T5 + G109
P45 + F289
Q98 + P473
G149 + F289
I214 + M246
S280 + P473

T5 + F113
P45 + V291
Q98 + C474
G149 + V291
I214 + M248
S280 + C474

T5 + R116
P45 + Q299
Q98 + G476
G149 + Q299
I214 + L250
S280 + G476

T5 + Q118
P45 + R320
Q98 + G477
G149 + R320
I214 + G255
S280 + G477

T5 + Q125
P45 + H321
Q98 + K484
G149 + H321
I214 + Q256
S280 + K484

T5 + G133
P45 + S323
M105 + G109
G149 + S323
I214 + A263
T285 + M286

T5 + T134
P45 + H324
M105 + F113
G149 + H324
I214 + V264
T285 + F289

T5 + W140
P45 + F328
M105 + R116
G149 + F328
I214 + Y267
T285 + V291

T5 + G142
P45 + T334
M105 + Q118
G149 + T334
I214 + N270
T285 + Q299

T5 + G149
P45 + D337
M105 + Q125
G149 + D337
I214 + G273
T285 + R320

T5 + T165
P45 + Q345
M105 + G133
G149 + Q345
I214 + S280
T285 + H321

T5 + W167
P45 + G346
M105 + T134
G149 + G346
I214 + T285
T285 + S323

T5 + R171
P45 + G348
M105 + W140
G149 + G348
I214 + M286
T285 + H324

T5 + Q172
P45 + T355
M105 + G142
G149 + T355
I214 + F289
T285 + F328

T5 + L173
P45 + S376
M105 + G149
G149 + S376
I214 + V291
T285 + T334

T5 + A174
P45 + D377
M105 + T165
G149 + D377
I214 + Q299
T285 + D337

T5 + G184
P45 + D379
M105 + W167
G149 + D379
I214 + R320
T285 + Q345

T5 + T193
P45 + Y382
M105 + R171
G149 + Y382
I214 + H321
T285 + G346

T5 + N195
P45 + S383
M105 + Q172
G149 + S383
I214 + S323
T285 + G348

T5 + A204
P45 + Q385
M105 + L173
G149 + Q385
I214 + H324
T285 + T355

T5 + V206
P45 + K391
M105 + A174
G149 + K391
I214 + F328
T285 + S376

T5 + P211
P45 + K393
M105 + G184
G149 + K393
I214 + T334
T285 + D377

T5 + I214
P45 + Q395
M105 + T193
G149 + Q395
I214 + D337
T285 + D379

T5 + V215
P45 + A420
M105 + N195
G149 + A420
I214 + Q345
T285 + Y382

T5 + L217
P45 + G423
M105 + A204
G149 + G423
I214 + G346
T285 + S383

T5 + L219
P45 + T444
M105 + V206
G149 + T444
I214 + G348
T285 + Q385

T5 + L235
P45 + A445
M105 + P211
G149 + A445
I214 + T355
T285 + K391

T5 + V238
P45 + Q449
M105 + I214
G149 + Q449
I214 + S376
T285 + K393

T5 + M246
P45 + T459
M105 + V215
G149 + T459
I214 + D377
T285 + Q395

T5 + M248
P45 + P473
M105 + L217
G149 + P473
I214 + D379
T285 + A420

T5 + L250
P45 + C474
M105 + L219
G149 + C474
I214 + Y382
T285 + G423

T5 + G255
P45 + G476
M105 + L235
G149 + G476
I214 + S383
T285 + T444

T5 + Q256
P45 + G477
M105 + V238
G149 + G477
I214 + Q385
T285 + A445

T5 + A263
P45 + K484
M105 + M246
G149 + K484
I214 + K391
T285 + Q449

T5 + V264
W48 + G50
M105 + M248
T165 + W167
I214 + K393
T285 + T459

T5 + Y267
W48 + T51
M105 + L250
T165 + R171
I214 + Q395
T285 + P473

T5 + N270
W48 + N54
M105 + G255
T165 + Q172
I214 + A420
T285 + C474

T5 + G273
W48 + V56
M105 + Q256
T165 + L173
I214 + G423
T285 + G476

T5 + S280
W48 + K72
M105 + A263
T165 + A174
I214 + T444
T285 + G477

T5 + T285
W48 + R87
M105 + V264
T165 + G184
I214 + A445
T285 + K484

T5 + M286
W48 + Q98
M105 + Y267
T165 + T193
I214 + Q449
M286 + F289

T5 + F289
W48 + M105
M105 + N270
T165 + N195
I214 + T459
M286 + V291

T5 + V291
W48 + G109
M105 + G273
T165 + A204
I214 + P473
M286 + Q299

T5 + Q299
W48 + F113
M105 + S280
T165 + V206
I214 + C474
M286 + R320

T5 + R320
W48 + R116
M105 + T285
T165 + P211
I214 + G476
M286 + H321

T5 + H321
W48 + Q118
M105 + M286
T165 + I214
I214 + G477
M286 + S323

T5 + S323
W48 + Q125
M105 + F289
T165 + V215
I214 + K484
M286 + H324

T5 + H324
W48 + G133
M105 + V291
T165 + L217
V215 + L217
M286 + F328

T5 + F328
W48 + T134
M105 + Q299
T165 + L219
V215 + L219
M286 + T334

T5 + T334
W48 + W140
M105 + R320
T165 + L235
V215 + L235
M286 + D337

T5 + D337
W48 + G142
M105 + H321
T165 + V238
V215 + V238
M286 + Q345

T5 + Q345
W48 + G149
M105 + S323
T165 + M246
V215 + M246
M286 + G346

T5 + G346
W48 + T165
M105 + H324
T165 + M248
V215 + M248
M286 + G348

T5 + G348
W48 + W167
M105 + F328
T165 + L250
V215 + L250
M286 + T355

T5 + T355
W48 + R171
M105 + T334
T165 + G255
V215 + G255
M286 + S376

T5 + S376
W48 + Q172
M105 + D337
T165 + Q256
V215 + Q256
M286 + D377

T5 + D377
W48 + L173
M105 + Q345
T165 + A263
V215 + A263
M286 + D379

T5 + D379
W48 + A174
M105 + G346
T165 + V264
V215 + V264
M286 + Y382

T5 + Y382
W48 + G184
M105 + G348
T165 + Y267
V215 + Y267
M286 + S383

T5 + S383
W48 + T193
M105 + T355
T165 + N270
V215 + N270
M286 + Q385

T5 + Q385
W48 + N195
M105 + S376
T165 + G273
V215 + G273
M286 + K391

T5 + K391
W48 + A204
M105 + D377
T165 + S280
V215 + S280
M286 + K393

T5 + K393
W48 + V206
M105 + D379
T165 + T285
V215 + T285
M286 + Q395

T5 + Q395
W48 + P211
M105 + Y382
T165 + M286
V215 + M286
M286 + A420

T5 + A420
W48 + I214
M105 + S383
T165 + F289
V215 + F289
M286 + G423

T5 + G423
W48 + V215
M105 + Q385
T165 + V291
V215 + V291
M286 + T444

T5 + T444
W48 + L217
M105 + K391
T165 + Q299
V215 + Q299
M286 + A445

T5 + A445
W48 + L219
M105 + K393
T165 + R320
V215 + R320
M286 + Q449

T5 + Q449
W48 + L235
M105 + Q395
T165 + H321
V215 + H321
M286 + T459

T5 + T459
W48 + V238
M105 + A420
T165 + S323
V215 + S323
M286 + P473

T5 + P473
W48 + M246
M105 + G423
T165 + H324
V215 + H324
M286 + C474

T5 + C474
W48 + M248
M105 + T444
T165 + F328
V215 + F328
M286 + G476

T5 + G476
W48 + L250
M105 + A445
T165 + T334
V215 + T334
M286 + G477

T5 + G477
W48 + G255
M105 + Q449
T165 + D337
V215 + D337
M286 + K484

T5 + K484
W48 + Q256
M105 + T459
T165 + Q345
V215 + Q345
F289 + V291

G7 + Q11
W48 + A263
M105 + P473
T165 + G346
V215 + G346
F289 + Q299

G7 + N16
W48 + V264
M105 + C474
T165 + G348
V215 + G348
F289 + R320

G7 + V17
W48 + Y267
M105 + G476
T165 + T355
V215 + T355
F289 + H321

G7 + Q32
W48 + N270
M105 + G477
T165 + S376
V215 + S376
F289 + S323

G7 + A37
W48 + G273
M105 + K484
T165 + D377
V215 + D377
F289 + H324

G7 + T40
W48 + S280
G109 + F113
T165 + D379
V215 + D379
F289 + F328

G7 + P45
W48 + T285
G109 + R116
T165 + Y382
V215 + Y382
F289 + T334

G7 + W48
W48 + M286
G109 + Q118
T165 + S383
V215 + S383
F289 + D337

G7 + G50
W48 + F289
G109 + Q125
T165 + Q385
V215 + Q385
F289 + Q345

G7 + T51
W48 + V291
G109 + G133
T165 + K391
V215 + K391
F289 + G346

G7 + N54
W48 + Q299
G109 + T134
T165 + K393
V215 + K393
F289 + G348

G7 + V56
W48 + R320
G109 + W140
T165 + Q395
V215 + Q395
F289 + T355

G7 + K72
W48 + H321
G109 + G142
T165 + A420
V215 + A420
F289 + S376

G7 + R87
W48 + S323
G109 + G149
T165 + G423
V215 + G423
F289 + D377

G7 + Q98
W48 + H324
G109 + T165
T165 + T444
V215 + T444
F289 + D379

G7 + M105
W48 + F328
G109 + W167
T165 + A445
V215 + A445
F289 + Y382

G7 + G109
W48 + T334
G109 + R171
T165 + Q449
V215 + Q449
F289 + S383

G7 + F113
W48 + D337
G109 + Q172
T165 + T459
V215 + T459
F289 + Q385

G7 + R116
W48 + Q345
G109 + L173
T165 + P473
V215 + P473
F289 + K391

G7 + Q118
W48 + G346
G109 + A174
T165 + C474
V215 + C474
F289 + K393

G7 + Q125
W48 + G348
G109 + G184
T165 + G476
V215 + G476
F289 + Q395

G7 + G133
W48 + T355
G109 + T193
T165 + G477
V215 + G477
F289 + A420

G7 + T134
W48 + S376
G109 + N195
T165 + K484
V215 + K484
F289 + G423

G7 + W140
W48 + D377
G109 + A204
W167 + R171
L217 + L219
F289 + T444

G7 + G142
W48 + D379
G109 + V206
W167 + Q172
L217 + L235
F289 + A445

G7 + G149
W48 + Y382
G109 + P211
W167 + L173
L217 + V238
F289 + Q449

G7 + T165
W48 + S383
G109 + I214
W167 + A174
L217 + M246
F289 + T459

G7 + W167
W48 + Q385
G109 + V215
W167 + G184
L217 + M248
F289 + P473

G7 + R171
W48 + K391
G109 + L217
W167 + T193
L217 + L250
F289 + C474

G7 + Q172
W48 + K393
G109 + L219
W167 + N195
L217 + G255
F289 + G476

G7 + L173
W48 + Q395
G109 + L235
W167 + A204
L217 + Q256
F289 + G477

G7 + A174
W48 + A420
G109 + V238
W167 + V206
L217 + A263
F289 + K484

G7 + G184
W48 + G423
G109 + M246
W167 + P211
L217 + V264
V291 + Q299

G7 + T193
W48 + T444
G109 + M248
W167 + I214
L217 + Y267
V291 + R320

G7 + N195
W48 + A445
G109 + L250
W167 + V215
L217 + N270
V291 + H321

G7 + A204
W48 + Q449
G109 + G255
W167 + L217
L217 + G273
V291 + S323

G7 + V206
W48 + T459
G109 + Q256
W167 + L219
L217 + S280
V291 + H324

G7 + P211
W48 + P473
G109 + A263
W167 + L235
L217 + T285
V291 + F328

G7 + I214
W48 + C474
G109 + V264
W167 + V238
L217 + M286
V291 + T334

G7 + V215
W48 + G476
G109 + Y267
W167 + M246
L217 + F289
V291 + D337

G7 + L217
W48 + G477
G109 + N270
W167 + M248
L217 + V291
V291 + Q345

G7 + L219
W48 + K484
G109 + G273
W167 + L250
L217 + Q299
V291 + G346

G7 + L235
G50 + T51
G109 + S280
W167 + G255
L217 + R320
V291 + G348

G7 + V238
G50 + N54
G109 + T285
W167 + Q256
L217 + H321
V291 + T355

G7 + M246
G50 + V56
G109 + M286
W167 + A263
L217 + S323
V291 + S376

G7 + M248
G50 + K72
G109 + F289
W167 + V264
L217 + H324
V291 + D377

G7 + L250
G50 + R87
G109 + V291
W167 + Y267
L217 + F328
V291 + D379

G7 + G255
G50 + Q98
G109 + Q299
W167 + N270
L217 + T334
V291 + Y382

G7 + Q256
G50 + M105
G109 + R320
W167 + G273
L217 + D337
V291 + S383

G7 + A263
G50 + G109
G109 + H321
W167 + S280
L217 + Q345
V291 + Q385

G7 + V264
G50 + F113
G109 + S323
W167 + T285
L217 + G346
V291 + K391

G7 + Y267
G50 + R116
G109 + H324
W167 + M286
L217 + G348
V291 + K393

G7 + N270
G50 + Q118
G109 + F328
W167 + F289
L217 + T355
V291 + Q395

G7 + G273
G50 + Q125
G109 + T334
W167 + V291
L217 + S376
V291 + A420

G7 + S280
G50 + G133
G109 + D337
W167 + Q299
L217 + D377
V291 + G423

G7 + T285
G50 + T134
G109 + Q345
W167 + R320
L217 + D379
V291 + T444

G7 + M286
G50 + W140
G109 + G346
W167 + H321
L217 + Y382
V291 + A445

G7 + F289
G50 + G142
G109 + G348
W167 + S323
L217 + S383
V291 + Q449

G7 + V291
G50 + G149
G109 + T355
W167 + H324
L217 + Q385
V291 + T459

G7 + Q299
G50 + T165
G109 + S376
W167 + F328
L217 + K391
V291 + P473

G7 + R320
G50 + W167
G109 + D377
W167 + T334
L217 + K393
V291 + C474

G7 + H321
G50 + R171
G109 + D379
W167 + D337
L217 + Q395
V291 + G476

G7 + S323
G50 + Q172
G109 + Y382
W167 + Q345
L217 + A420
V291 + G477

G7 + H324
G50 + L173
G109 + S383
W167 + G346
L217 + G423
V291 + K484

G7 + F328
G50 + A174
G109 + Q385
W167 + G348
L217 + T444
Q299 + R320

G7 + T334
G50 + G184
G109 + K391
W167 + T355
L217 + A445
Q299 + H321

G7 + D337
G50 + T193
G109 + K393
W167 + S376
L217 + Q449
Q299 + S323

G7 + Q345
G50 + N195
G109 + Q395
W167 + D377
L217 + T459
Q299 + H324

G7 + G346
G50 + A204
G109 + A420
W167 + D379
L217 + P473
Q299 + F328

G7 + G348
G50 + V206
G109 + G423
W167 + Y382
L217 + C474
Q299 + T334

G7 + T355
G50 + P211
G109 + T444
W167 + S383
L217 + G476
Q299 + D337

G7 + S376
G50 + I214
G109 + A445
W167 + Q385
L217 + G477
Q299 + Q345

G7 + D377
G50 + V215
G109 + Q449
W167 + K391
L217 + K484
Q299 + G346

G7 + D379
G50 + L217
G109 + T459
W167 + K393
L219 + L235
Q299 + G348

G7 + Y382
G50 + L219
G109 + P473
W167 + Q395
L219 + V238
Q299 + T355

G7 + S383
G50 + L235
G109 + C474
W167 + A420
L219 + M246
Q299 + S376

G7 + Q385
G50 + V238
G109 + G476
W167 + G423
L219 + M248
Q299 + D377

G7 + K391
G50 + M246
G109 + G477
W167 + T444
L219 + L250
Q299 + D379

G7 + K393
G50 + M248
G109 + K484
W167 + A445
L219 + G255
Q299 + Y382

G7 + Q395
G50 + L250
F113 + R116
W167 + Q449
L219 + Q256
Q299 + S383

G7 + A420
G50 + G255
F113 + Q118
W167 + T459
L219 + A263
Q299 + Q385

G7 + G423
G50 + Q256
F113 + Q125
W167 + P473
L219 + V264
Q299 + K391

G7 + T444
G50 + A263
F113 + G133
W167 + C474
L219 + Y267
Q299 + K393

G7 + A445
G50 + V264
F113 + T134
W167 + G476
L219 + N270
Q299 + Q395

G7 + Q449
G50 + Y267
F113 + W140
W167 + G477
L219 + G273
Q299 + A420

G7 + T459
G50 + N270
F113 + G142
W167 + K484
L219 + S280
Q299 + G423

G7 + P473
G50 + G273
F113 + G149
R171 + Q172
L219 + T285
Q299 + T444

G7 + C474
G50 + S280
F113 + T165
R171 + L173
L219 + M286
Q299 + A445

G7 + G476
G50 + T285
F113 + W167
R171 + A174
L219 + F289
Q299 + Q449

G7 + G477
G50 + M286
F113 + R171
R171 + G184
L219 + V291
Q299 + T459

G7 + K484
G50 + F289
F113 + Q172
R171 + T193
L219 + Q299
Q299 + P473

Q11 + N16
G50 + V291
F113 + L173
R171 + N195
L219 + R320
Q299 + C474

Q11 + V17
G50 + Q299
F113 + A174
R171 + A204
L219 + H321
Q299 + G476

Q11 + Q32
G50 + R320
F113 + G184
R171 + V206
L219 + S323
Q299 + G477

Q11 + A37
G50 + H321
F113 + T193
R171 + P211
L219 + H324
Q299 + K484

Q11 + T40
G50 + S323
F113 + N195
R171 + I214
L219 + F328
R320 + H321

Q11 + P45
G50 + H324
F113 + A204
R171 + V215
L219 + T334
R320 + S323

Q11 + W48
G50 + F328
F113 + V206
R171 + L217
L219 + D337
R320 + H324

Q11 + G50
G50 + T334
F113 + P211
R171 + L219
L219 + Q345
R320 + F328

Q11 + T51
G50 + D337
F113 + I214
R171 + L235
L219 + G346
R320 + T334

Q11 + N54
G50 + Q345
F113 + V215
R171 + V238
L219 + G348
R320 + D337

Q11 + V56
G50 + G346
F113 + L217
R171 + M246
L219 + T355
R320 + Q345

Q11 + K72
G50 + G348
F113 + L219
R171 + M248
L219 + S376
R320 + G346

Q11 + R87
G50 + T355
F113 + L235
R171 + L250
L219 + D377
R320 + G348

Q11 + Q98
G50 + S376
F113 + V238
R171 + G255
L219 + D379
R320 + T355

Q11 + M105
G50 + D377
F113 + M246
R171 + Q256
L219 + Y382
R320 + S376

Q11 + G109
G50 + D379
F113 + M248
R171 + A263
L219 + S383
R320 + D377

Q11 + F113
G50 + Y382
F113 + L250
R171 + V264
L219 + Q385
R320 + D379

Q11 + R116
G50 + S383
F113 + G255
R171 + Y267
L219 + K391
R320 + Y382

Q11 + Q118
G50 + Q385
F113 + Q256
R171 + N270
L219 + K393
R320 + S383

Q11 + Q125
G50 + K391
F113 + A263
R171 + G273
L219 + Q395
R320 + Q385

Q11 + G133
G50 + K393
F113 + V264
R171 + S280
L219 + A420
R320 + K391

Q11 + T134
G50 + Q395
F113 + Y267
R171 + T285
L219 + G423
R320 + K393

Q11 + W140
G50 + A420
F113 + N270
R171 + M286
L219 + T444
R320 + Q395

Q11 + G142
G50 + G423
F113 + G273
R171 + F289
L219 + A445
R320 + A420

Q11 + G149
G50 + T444
F113 + S280
R171 + V291
L219 + Q449
R320 + G423

Q11 + T165
G50 + A445
F113 + T285
R171 + Q299
L219 + T459
R320 + T444

Q11 + W167
G50 + Q449
F113 + M286
R171 + R320
L219 + P473
R320 + A445

Q11 + R171
G50 + T459
F113 + F289
R171 + H321
L219 + C474
R320 + Q449

Q11 + Q172
G50 + P473
F113 + V291
R171 + S323
L219 + G476
R320 + T459

Q11 + L173
G50 + C474
F113 + Q299
R171 + H324
L219 + G477
R320 + P473

Q11 + A174
G50 + G476
F113 + R320
R171 + F328
L219 + K484
R320 + C474

Q11 + G184
G50 + G477
F113 + H321
R171 + T334
L235 + V238
R320 + G476

Q11 + T193
G50 + K484
F113 + S323
R171 + D337
L235 + M246
R320 + G477

Q11 + N195
T51 + N54
F113 + H324
R171 + Q345
L235 + M248
R320 + K484

Q11 + A204
T51 + V56
F113 + F328
R171 + G346
L235 + L250
H321 + S323

Q11 + V206
T51 + K72
F113 + T334
R171 + G348
L235 + G255
H321 + H324

Q11 + P211
T51 + R87
F113 + D337
R171 + T355
L235 + Q256
H321 + F328

Q11 + I214
T51 + Q98
F113 + Q345
R171 + S376
L235 + A263
H321 + T334

Q11 + V215
T51 + M105
F113 + G346
R171 + D377
L235 + V264
H321 + D337

Q11 + L217
T51 + G109
F113 + G348
R171 + D379
L235 + Y267
H321 + Q345

Q11 + L219
T51 + F113
F113 + T355
R171 + Y382
L235 + N270
H321 + G346

Q11 + L235
T51 + R116
F113 + S376
R171 + S383
L235 + G273
H321 + G348

Q11 + V238
T51 + Q118
F113 + D377
R171 + Q385
L235 + S280
H321 + T355

Q11 + M246
T51 + Q125
F113 + D379
R171 + K391
L235 + T285
H321 + S376

Q11 + M248
T51 + G133
F113 + Y382
R171 + K393
L235 + M286
H321 + D377

Q11 + L250
T51 + T134
F113 + S383
R171 + Q395
L235 + F289
H321 + D379

Q11 + G255
T51 + W140
F113 + Q385
R171 + A420
L235 + V291
H321 + Y382

Q11 + Q256
T51 + G142
F113 + K391
R171 + G423
L235 + Q299
H321 + S383

Q11 + A263
T51 + G149
F113 + K393
R171 + T444
L235 + R320
H321 + Q385

Q11 + V264
T51 + T165
F113 + Q395
R171 + A445
L235 + H321
H321 + K391

Q11 + Y267
T51 + W167
F113 + A420
R171 + Q449
L235 + S323
H321 + K393

Q11 + N270
T51 + R171
F113 + G423
R171 + T459
L235 + H324
H321 + Q395

Q11 + G273
T51 + Q172
F113 + T444
R171 + P473
L235 + F328
H321 + A420

Q11 + S280
T51 + L173
F113 + A445
R171 + C474
L235 + T334
H321 + G423

Q11 + T285
T51 + A174
F113 + Q449
R171 + G476
L235 + D337
H321 + T444

Q11 + M286
T51 + G184
F113 + T459
R171 + G477
L235 + Q345
H321 + A445

Q11 + F289
T51 + T193
F113 + P473
R171 + K484
L235 + G346
H321 + Q449

Q11 + V291
T51 + N195
F113 + C474
Q172 + L173
L235 + G348
H321 + T459

Q11 + Q299
T51 + A204
F113 + G476
Q172 + A174
L235 + T355
H321 + P473

Q11 + R320
T51 + V206
F113 + G477
Q172 + G184
L235 + S376
H321 + C474

Q11 + H321
T51 + P211
F113 + K484
Q172 + T193
L235 + D377
H321 + G476

Q11 + S323
T51 + I214
R116 + Q118
Q172 + N195
L235 + D379
H321 + G477

Q11 + H324
T51 + V215
R116 + Q125
Q172 + A204
L235 + Y382
H321 + K484

Q11 + F328
T51 + L217
R116 + G133
Q172 + V206
L235 + S383
S323 + H324

Q11 + T334
T51 + L219
R116 + T134
Q172 + P211
L235 + Q385
S323 + F328

Q11 + D337
T51 + L235
R116 + W140
Q172 + I214
L235 + K391
S323 + T334

Q11 + Q345
T51 + V238
R116 + G142
Q172 + V215
L235 + K393
S323 + D337

Q11 + G346
T51 + M246
R116 + G149
Q172 + L217
L235 + Q395
S323 + Q345

Q11 + G348
T51 + M248
R116 + T165
Q172 + L219
L235 + A420
S323 + G346

Q11 + T355
T51 + L250
R116 + W167
Q172 + L235
L235 + G423
S323 + G348

Q11 + S376
T51 + G255
R116 + R171
Q172 + V238
L235 + T444
S323 + T355

Q11 + D377
T51 + Q256
R116 + Q172
Q172 + M246
L235 + A445
S323 + S376

Q11 + D379
T51 + A263
R116 + L173
Q172 + M248
L235 + Q449
S323 + D377

Q11 + Y382
T51 + V264
R116 + A174
Q172 + L250
L235 + T459
S323 + D379

Q11 + S383
T51 + Y267
R116 + G184
Q172 + G255
L235 + P473
S323 + Y382

Q11 + Q385
T51 + N270
R116 + T193
Q172 + Q256
L235 + C474
S323 + S383

Q11 + K391
T51 + G273
R116 + N195
Q172 + A263
L235 + G476
S323 + Q385

Q11 + K393
T51 + S280
R116 + A204
Q172 + V264
L235 + G477
S323 + K391

Q11 + Q395
T51 + T285
R116 + V206
Q172 + Y267
L235 + K484
S323 + K393

Q11 + A420
T51 + M286
R116 + P211
Q172 + N270
V238 + M246
S323 + Q395

Q11 + G423
T51 + F289
R116 + I214
Q172 + G273
V238 + M248
S323 + A420

Q11 + T444
T51 + V291
R116 + V215
Q172 + S280
V238 + L250
S323 + G423

Q11 + A445
T51 + Q299
R116 + L217
Q172 + T285
V238 + G255
S323 + T444

Q11 + Q449
T51 + R320
R116 + L219
Q172 + M286
V238 + Q256
S323 + A445

Q11 + T459
T51 + H321
R116 + L235
Q172 + F289
V238 + A263
S323 + Q449

Q11 + P473
T51 + S323
R116 + V238
Q172 + V291
V238 + V264
S323 + T459

Q11 + C474
T51 + H324
R116 + M246
Q172 + Q299
V238 + Y267
S323 + P473

Q11 + G476
T51 + F328
R116 + M248
Q172 + R320
V238 + N270
S323 + C474

Q11 + G477
T51 + T334
R116 + L250
Q172 + H321
V238 + G273
S323 + G476

Q11 + K484
T51 + D337
R116 + G255
Q172 + S323
V238 + S280
S323 + G477

N16 + V17
T51 + Q345
R116 + Q256
Q172 + H324
V238 + T285
S323 + K484

N16 + Q32
T51 + G346
R116 + A263
Q172 + F328
V238 + M286
H324 + F328

N16 + A37
T51 + G348
R116 + V264
Q172 + T334
V238 + F289
H324 + T334

N16 + T40
T51 + T355
R116 + Y267
Q172 + D337
V238 + V291
H324 + D337

N16 + P45
T51 + S376
R116 + N270
Q172 + Q345
V238 + Q299
H324 + Q345

N16 + W48
T51 + D377
R116 + G273
Q172 + G346
V238 + R320
H324 + G346

N16 + G50
T51 + D379
R116 + S280
Q172 + G348
V238 + H321
H324 + G348

N16 + T51
T51 + Y382
R116 + T285
Q172 + T355
V238 + S323
H324 + T355

N16 + N54
T51 + S383
R116 + M286
Q172 + S376
V238 + H324
H324 + S376

N16 + V56
T51 + Q385
R116 + F289
Q172 + D377
V238 + F328
H324 + D377

N16 + K72
T51 + K391
R116 + V291
Q172 + D379
V238 + T334
H324 + D379

N16 + R87
T51 + K393
R116 + Q299
Q172 + Y382
V238 + D337
H324 + Y382

N16 + Q98
T51 + Q395
R116 + R320
Q172 + S383
V238 + Q345
H324 + S383

N16 + M105
T51 + A420
R116 + H321
Q172 + Q385
V238 + G346
H324 + Q385

N16 + G109
T51 + G423
R116 + S323
Q172 + K391
V238 + G348
H324 + K391

N16 + F113
T51 + T444
R116 + H324
Q172 + K393
V238 + T355
H324 + K393

N16 + R116
T51 + A445
R116 + F328
Q172 + Q395
V238 + S376
H324 + Q395

N16 + Q118
T51 + Q449
R116 + T334
Q172 + A420
V238 + D377
H324 + A420

N16 + Q125
T51 + T459
R116 + D337
Q172 + G423
V238 + D379
H324 + G423

N16 + G133
T51 + P473
R116 + Q345
Q172 + T444
V238 + Y382
H324 + T444

N16 + T134
T51 + C474
R116 + G346
Q172 + A445
V238 + S383
H324 + A445

N16 + W140
T51 + G476
R116 + G348
Q172 + Q449
V238 + Q385
H324 + Q449

N16 + G142
T51 + G477
R116 + T355
Q172 + T459
V238 + K391
H324 + T459

N16 + G149
T51 + K484
R116 + S376
Q172 + P473
V238 + K393
H324 + P473

N16 + T165
N54 + V56
R116 + D377
Q172 + C474
V238 + Q395
H324 + C474

N16 + W167
N54 + K72
R116 + D379
Q172 + G476
V238 + A420
H324 + G476

N16 + R171
N54 + R87
R116 + Y382
Q172 + G477
V238 + G423
H324 + G477

N16 + Q172
N54 + Q98
R116 + S383
Q172 + K484
V238 + T444
H324 + K484

N16 + L173
N54 + M105
R116 + Q385
L173 + A174
V238 + A445
F328 + T334

N16 + A174
N54 + G109
R116 + K391
L173 + G184
V238 + Q449
F328 + D337

N16 + G184
N54 + F113
R116 + K393
L173 + T193
V238 + T459
F328 + Q345

N16 + T193
N54 + R116
R116 + Q395
L173 + N195
V238 + P473
F328 + G346

N16 + N195
N54 + Q118
R116 + A420
L173 + A204
V238 + C474
F328 + G348

N16 + A204
N54 + Q125
R116 + G423
L173 + V206
V238 + G476
F328 + T355

N16 + V206
N54 + G133
R116 + T444
L173 + P211
V238 + G477
F328 + S376

N16 + P211
N54 + T134
R116 + A445
L173 + I214
V238 + K484
F328 + D377

N16 + I214
N54 + W140
R116 + Q449
L173 + V215
M246 + M248
F328 + D379

N16 + V215
N54 + G142
R116 + T459
L173 + L217
M246 + L250
F328 + Y382

N16 + L217
N54 + G149
R116 + P473
L173 + L219
M246 + G255
F328 + S383

N16 + L219
N54 + T165
R116 + C474
L173 + L235
M246 + Q256
F328 + Q385

N16 + L235
N54 + W167
R116 + G476
L173 + V238
M246 + A263
F328 + K391

N16 + V238
N54 + R171
R116 + G477
L173 + M246
M246 + V264
F328 + K393

N16 + M246
N54 + Q172
R116 + K484
L173 + M248
M246 + Y267
F328 + Q395

N16 + M248
N54 + L173
Q118 + Q125
L173 + L250
M246 + N270
F328 + A420

N16 + L250
N54 + A174
Q118 + G133
L173 + G255
M246 + G273
F328 + G423

N16 + G255
N54 + G184
Q118 + T134
L173 + Q256
M246 + S280
F328 + T444

N16 + Q256
N54 + T193
Q118 + W140
L173 + A263
M246 + T285
F328 + A445

N16 + A263
N54 + N195
Q118 + G142
L173 + V264
M246 + M286
F328 + Q449

N16 + V264
N54 + A204
Q118 + G149
L173 + Y267
M246 + F289
F328 + T459

N16 + Y267
N54 + V206
Q118 + T165
L173 + N270
M246 + V291
F328 + P473

N16 + N270
N54 + P211
Q118 + W167
L173 + G273
M246 + Q299
F328 + C474

N16 + G273
N54 + I214
Q118 + R171
L173 + S280
M246 + R320
F328 + G476

N16 + S280
N54 + V215
Q118 + Q172
L173 + T285
M246 + H321
F328 + G477

N16 + T285
N54 + L217
Q118 + L173
L173 + M286
M246 + S323
F328 + K484

N16 + M286
N54 + L219
Q118 + A174
L173 + F289
M246 + H324
T334 + D337

N16 + F289
N54 + L235
Q118 + G184
L173 + V291
M246 + F328
T334 + Q345

N16 + V291
N54 + V238
Q118 + T193
L173 + Q299
M246 + T334
T334 + G346

N16 + Q299
N54 + M246
Q118 + N195
L173 + R320
M246 + D337
T334 + G348

N16 + R320
N54 + M248
Q118 + A204
L173 + H321
M246 + Q345
T334 + T355

N16 + H321
N54 + L250
Q118 + V206
L173 + S323
M246 + G346
T334 + S376

N16 + S323
N54 + G255
Q118 + P211
L173 + H324
M246 + G348
T334 + D377

N16 + H324
N54 + Q256
Q118 + I214
L173 + F328
M246 + T355
T334 + D379

N16 + F328
N54 + A263
Q118 + V215
L173 + T334
M246 + S376
T334 + Y382

N16 + T334
N54 + V264
Q118 + L217
L173 + D337
M246 + D377
T334 + S383

N16 + D337
N54 + Y267
Q118 + L219
L173 + Q345
M246 + D379
T334 + Q385

N16 + Q345
N54 + N270
Q118 + L235
L173 + G346
M246 + Y382
T334 + K391

N16 + G346
N54 + G273
Q118 + V238
L173 + G348
M246 + S383
T334 + K393

N16 + G348
N54 + S280
Q118 + M246
L173 + T355
M246 + Q385
T334 + Q395

N16 + T355
N54 + T285
Q118 + M248
L173 + S376
M246 + K391
T334 + A420

N16 + S376
N54 + M286
Q118 + L250
L173 + D377
M246 + K393
T334 + G423

N16 + D377
N54 + F289
Q118 + G255
L173 + D379
M246 + Q395
T334 + T444

N16 + D379
N54 + V291
Q118 + Q256
L173 + Y382
M246 + A420
T334 + A445

N16 + Y382
N54 + Q299
Q118 + A263
L173 + S383
M246 + G423
T334 + Q449

N16 + S383
N54 + R320
Q118 + V264
L173 + Q385
M246 + T444
T334 + T459

N16 + Q385
N54 + H321
Q118 + Y267
L173 + K391
M246 + A445
T334 + P473

N16 + K391
N54 + S323
Q118 + N270
L173 + K393
M246 + Q449
T334 + C474

N16 + K393
N54 + H324
Q118 + G273
L173 + Q395
M246 + T459
T334 + G476

N16 + Q395
N54 + F328
Q118 + S280
L173 + A420
M246 + P473
T334 + G477

N16 + A420
N54 + T334
Q118 + T285
L173 + G423
M246 + C474
T334 + K484

N16 + G423
N54 + D337
Q118 + M286
L173 + T444
M246 + G476
D337 + Q345

N16 + T444
N54 + Q345
Q118 + F289
L173 + A445
M246 + G477
D337 + G346

N16 + A445
N54 + G346
Q118 + V291
L173 + Q449
M246 + K484
D337 + G348

N16 + Q449
N54 + G348
Q118 + Q299
L173 + T459
M248 + L250
D337 + T355

N16 + T459
N54 + T355
Q118 + R320
L173 + P473
M248 + G255
D337 + S376

N16 + P473
N54 + S376
Q118 + H321
L173 + C474
M248 + Q256
D337 + D377

N16 + C474
N54 + D377
Q118 + S323
L173 + G476
M248 + A263
D337 + D379

N16 + G476
N54 + D379
Q118 + H324
L173 + G477
M248 + V264
D337 + Y382

N16 + G477
N54 + Y382
Q118 + F328
L173 + K484
M248 + Y267
D337 + S383

N16 + K484
N54 + S383
Q118 + T334
A174 + G184
M248 + N270
D337 + Q385

V17 + Q32
N54 + Q385
Q118 + D337
A174 + T193
M248 + G273
D337 + K391

V17 + A37
N54 + K391
Q118 + Q345
A174 + N195
M248 + S280
D337 + K393

V17 + T40
N54 + K393
Q118 + G346
A174 + A204
M248 + T285
D337 + Q395

V17 + P45
N54 + Q395
Q118 + G348
A174 + V206
M248 + M286
D337 + A420

V17 + W48
N54 + A420
Q118 + T355
A174 + P211
M248 + F289
D337 + G423

V17 + G50
N54 + G423
Q118 + S376
A174 + I214
M248 + V291
D337 + T444

V17 + T51
N54 + T444
Q118 + D377
A174 + V215
M248 + Q299
D337 + A445

V17 + N54
N54 + A445
Q118 + D379
A174 + L217
M248 + R320
D337 + Q449

V17 + V56
N54 + Q449
Q118 + Y382
A174 + L219
M248 + H321
D337 + T459

V17 + K72
N54 + T459
Q118 + S383
A174 + L235
M248 + S323
D337 + P473

V17 + R87
N54 + P473
Q118 + Q385
A174 + V238
M248 + H324
D337 + C474

V17 + Q98
N54 + C474
Q118 + K391
A174 + M246
M248 + F328
D337 + G476

V17 + M105
N54 + G476
Q118 + K393
A174 + M248
M248 + T334
D337 + G477

V17 + G109
N54 + G477
Q118 + Q395
A174 + L250
M248 + D337
D337 + K484

V17 + F113
N54 + K484
Q118 + A420
A174 + G255
M248 + Q345
Q345 + G346

V17 + R116
V56 + K72
Q118 + G423
A174 + Q256
M248 + G346
Q345 + G348

V17 + Q118
V56 + R87
Q118 + T444
A174 + A263
M248 + G348
Q345 + T355

V17 + Q125
V56 + Q98
Q118 + A445
A174 + V264
M248 + T355
Q345 + S376

V17 + G133
V56 + M105
Q118 + Q449
A174 + Y267
M248 + S376
Q345 + D377

V17 + T134
V56 + G109
Q118 + T459
A174 + N270
M248 + D377
Q345 + D379

V17 + W140
V56 + F113
Q118 + P473
A174 + G273
M248 + D379
Q345 + Y382

V17 + G142
V56 + R116
Q118 + C474
A174 + S280
M248 + Y382
Q345 + S383

V17 + G149
V56 + Q118
Q118 + G476
A174 + T285
M248 + S383
Q345 + Q385

V17 + T165
V56 + Q125
Q118 + G477
A174 + M286
M248 + Q385
Q345 + K391

V17 + W167
V56 + G133
Q118 + K484
A174 + F289
M248 + K391
Q345 + K393

V17 + R171
V56 + T134
Q125 + G133
A174 + V291
M248 + K393
Q345 + Q395

V17 + Q172
V56 + W140
Q125 + T134
A174 + Q299
M248 + Q395
Q345 + A420

V17 + L173
V56 + G142
Q125 + W140
A174 + R320
M248 + A420
Q345 + G423

V17 + A174
V56 + G149
Q125 + G142
A174 + H321
M248 + G423
Q345 + T444

V17 + G184
V56 + T165
Q125 + G149
A174 + S323
M248 + T444
Q345 + A445

V17 + T193
V56 + W167
Q125 + T165
A174 + H324
M248 + A445
Q345 + Q449

V17 + N195
V56 + R171
Q125 + W167
A174 + F328
M248 + Q449
Q345 + T459

V17 + A204
V56 + Q172
Q125 + R171
A174 + T334
M248 + T459
Q345 + P473

V17 + V206
V56 + L173
Q125 + Q172
A174 + D337
M248 + P473
Q345 + C474

V17 + P211
V56 + A174
Q125 + L173
A174 + Q345
M248 + C474
Q345 + G476

V17 + I214
V56 + G184
Q125 + A174
A174 + G346
M248 + G476
Q345 + G477

V17 + V215
V56 + T193
Q125 + G184
A174 + G348
M248 + G477
Q345 + K484

V17 + L217
V56 + N195
Q125 + T193
A174 + T355
M248 + K484
G346 + G348

V17 + L219
V56 + A204
Q125 + N195
A174 + S376
L250 + G255
G346 + T355

V17 + L235
V56 + V206
Q125 + A204
A174 + D377
L250 + Q256
G346 + S376

V17 + V238
V56 + P211
Q125 + V206
A174 + D379
L250 + A263
G346 + D377

V17 + M246
V56 + I214
Q125 + P211
A174 + Y382
L250 + V264
G346 + D379

V17 + M248
V56 + V215
Q125 + I214
A174 + S383
L250 + Y267
G346 + Y382

V17 + L250
V56 + L217
Q125 + V215
A174 + Q385
L250 + N270
G346 + S383

V17 + G255
V56 + L219
Q125 + L217
A174 + K391
L250 + G273
G346 + Q385

V17 + Q256
V56 + L235
Q125 + L219
A174 + K393
L250 + S280
G346 + K391

V17 + A263
V56 + V238
Q125 + L235
A174 + Q395
L250 + T285
G346 + K393

V17 + V264
V56 + M246
Q125 + V238
A174 + A420
L250 + M286
G346 + Q395

V17 + Y267
V56 + M248
Q125 + M246
A174 + G423
L250 + F289
G346 + A420

V17 + N270
V56 + L250
Q125 + M248
A174 + T444
L250 + V291
G346 + G423

V17 + G273
V56 + G255
Q125 + L250
A174 + A445
L250 + Q299
G346 + T444

V17 + S280
V56 + Q256
Q125 + G255
A174 + Q449
L250 + R320
G346 + A445

V17 + T285
V56 + A263
Q125 + Q256
A174 + T459
L250 + H321
G346 + Q449

V17 + M286
V56 + V264
Q125 + A263
A174 + P473
L250 + S323
G346 + T459

V17 + F289
V56 + Y267
Q125 + V264
A174 + C474
L250 + H324
G346 + P473

V17 + V291
V56 + N270
Q125 + Y267
A174 + G476
L250 + F328
G346 + C474

V17 + Q299
V56 + G273
Q125 + N270
A174 + G477
L250 + T334
G346 + G476

V17 + R320
V56 + S280
Q125 + G273
A174 + K484
L250 + D337
G346 + G477

V17 + H321
V56 + T285
Q125 + S280
G184 + T193
L250 + Q345
G346 + K484

V17 + S323
V56 + M286
Q125 + T285
G184 + N195
L250 + G346
G348 + T355

V17 + H324
V56 + F289
Q125 + M286
G184 + A204
L250 + G348
G348 + S376

V17 + F328
V56 + V291
Q125 + F289
G184 + V206
L250 + T355
G348 + D377

V17 + T334
V56 + Q299
Q125 + V291
G184 + P211
L250 + S376
G348 + D379

V17 + D337
V56 + R320
Q125 + Q299
G184 + I214
L250 + D377
G348 + Y382

V17 + Q345
V56 + H321
Q125 + R320
G184 + V215
L250 + D379
G348 + S383

V17 + G346
V56 + S323
Q125 + H321
G184 + L217
L250 + Y382
G348 + Q385

V17 + G348
V56 + H324
Q125 + S323
G184 + L219
L250 + S383
G348 + K391

V17 + T355
V56 + F328
Q125 + H324
G184 + L235
L250 + Q385
G348 + K393

V17 + S376
V56 + T334
Q125 + F328
G184 + V238
L250 + K391
G348 + Q395

V17 + D377
V56 + D337
Q125 + T334
G184 + M246
L250 + K393
G348 + A420

V17 + D379
V56 + Q345
Q125 + D337
G184 + M248
L250 + Q395
G348 + G423

V17 + Y382
V56 + G346
Q125 + Q345
G184 + L250
L250 + A420
G348 + T444

V17 + S383
V56 + G348
Q125 + G346
G184 + G255
L250 + G423
G348 + A445

V17 + Q385
V56 + T355
Q125 + G348
G184 + Q256
L250 + T444
G348 + Q449

V17 + K391
V56 + S376
Q125 + T355
G184 + A263
L250 + A445
G348 + T459

V17 + K393
V56 + D377
Q125 + S376
G184 + V264
L250 + Q449
G348 + P473

V17 + Q395
V56 + D379
Q125 + D377
G184 + Y267
L250 + T459
G348 + C474

V17 + A420
V56 + Y382
Q125 + D379
G184 + N270
L250 + P473
G348 + G476

V17 + G423
V56 + S383
Q125 + Y382
G184 + G273
L250 + C474
G348 + G477

V17 + T444
V56 + Q385
Q125 + S383
G184 + S280
L250 + G476
G348 + K484

V17 + A445
V56 + K391
Q125 + Q385
G184 + T285
L250 + G477
T355 + S376

V17 + Q449
V56 + K393
Q125 + K391
G184 + M286
L250 + K484
T355 + D377

V17 + T459
V56 + Q395
Q125 + K393
G184 + F289
G255 + Q256
T355 + D379

V17 + P473
V56 + A420
Q125 + Q395
G184 + V291
G255 + A263
T355 + Y382

V17 + C474
V56 + G423
Q125 + A420
G184 + Q299
G255 + V264
T355 + S383

V17 + G476
V56 + T444
Q125 + G423
G184 + R320
G255 + Y267
T355 + Q385

V17 + G477
V56 + A445
Q125 + T444
G184 + H321
G255 + N270
T355 + K391

V17 + K484
V56 + Q449
Q125 + A445
G184 + S323
G255 + G273
T355 + K393

Q32 + A37
V56 + T459
Q125 + Q449
G184 + H324
G255 + S280
T355 + Q395

Q32 + T40
V56 + P473
Q125 + T459
G184 + F328
G255 + T285
T355 + A420

Q32 + P45
V56 + C474
Q125 + P473
G184 + T334
G255 + M286
T355 + G423

Q32 + W48
V56 + G476
Q125 + C474
G184 + D337
G255 + F289
T355 + T444

Q32 + G50
V56 + G477
Q125 + G476
G184 + Q345
G255 + V291
T355 + A445

Q32 + T51
V56 + K484
Q125 + G477
G184 + G346
G255 + Q299
T355 + Q449

Q32 + N54
K72 + R87
Q125 + K484
G184 + G348
G255 + R320
T355 + T459

Q32 + V56
K72 + Q98
G133 + T134
G184 + T355
G255 + H321
T355 + P473

Q32 + K72
K72 + M105
G133 + W140
G184 + S376
G255 + S323
T355 + C474

Q32 + R87
K72 + G109
G133 + G142
G184 + D377
G255 + H324
T355 + G476

Q32 + Q98
K72 + F113
G133 + G149
G184 + D379
G255 + F328
T355 + G477

Q32 + M105
K72 + R116
G133 + T165
G184 + Y382
G255 + T334
T355 + K484

Q32 + G109
K72 + Q118
G133 + W167
G184 + S383
G255 + D337
S376 + D377

Q32 + F113
K72 + Q125
G133 + R171
G184 + Q385
G255 + Q345
S376 + D379

Q32 + R116
K72 + G133
G133 + Q172
G184 + K391
G255 + G346
S376 + Y382

Q32 + Q118
K72 + T134
G133 + L173
G184 + K393
G255 + G348
S376 + S383

Q32 + Q125
K72 + W140
G133 + A174
G184 + Q395
G255 + T355
S376 + Q385

Q32 + G133
K72 + G142
G133 + G184
G184 + A420
G255 + S376
S376 + K391

Q32 + T134
K72 + G149
G133 + T193
G184 + G423
G255 + D377
S376 + K393

Q32 + W140
K72 + T165
G133 + N195
G184 + T444
G255 + D379
S376 + Q395

Q32 + G142
K72 + W167
G133 + A204
G184 + A445
G255 + Y382
S376 + A420

Q32 + G149
K72 + R171
G133 + V206
G184 + Q449
G255 + S383
S376 + G423

Q32 + T165
K72 + Q172
G133 + P211
G184 + T459
G255 + Q385
S376 + T444

Q32 + W167
K72 + L173
G133 + I214
G184 + P473
G255 + K391
S376 + A445

Q32 + R171
K72 + A174
G133 + V215
G184 + C474
G255 + K393
S376 + Q449

Q32 + Q172
K72 + G184
G133 + L217
G184 + G476
G255 + Q395
S376 + T459

Q32 + L173
K72 + T193
G133 + L219
G184 + G477
G255 + A420
S376 + P473

Q32 + A174
K72 + N195
G133 + L235
G184 + K484
G255 + G423
S376 + C474

Q32 + G184
K72 + A204
G133 + V238
T193 + N195
G255 + T444
S376 + G476

Q32 + T193
K72 + V206
G133 + M246
T193 + A204
G255 + A445
S376 + G477

Q32 + N195
K72 + P211
G133 + M248
T193 + V206
G255 + Q449
S376 + K484

Q32 + A204
K72 + I214
G133 + L250
T193 + P211
G255 + T459
D377 + D379

Q32 + V206
K72 + V215
G133 + G255
T193 + I214
G255 + P473
D377 + Y382

Q32 + P211
K72 + L217
G133 + Q256
T193 + V215
G255 + C474
D377 + S383

Q32 + I214
K72 + L219
G133 + A263
T193 + L217
G255 + G476
D377 + Q385

Q32 + V215
K72 + L235
G133 + V264
T193 + L219
G255 + G477
D377 + K391

Q32 + L217
K72 + V238
G133 + Y267
T193 + L235
G255 + K484
D377 + K393

Q32 + L219
K72 + M246
G133 + N270
T193 + V238
Q256 + A263
D377 + Q395

Q32 + L235
K72 + M248
G133 + G273
T193 + M246
Q256 + V264
D377 + A420

Q32 + V238
K72 + L250
G133 + S280
T193 + M248
Q256 + Y267
D377 + G423

Q32 + M246
K72 + G255
G133 + T285
T193 + L250
Q256 + N270
D377 + T444

Q32 + M248
K72 + Q256
G133 + M286
T193 + G255
Q256 + G273
D377 + A445

Q32 + L250
K72 + A263
G133 + F289
T193 + Q256
Q256 + S280
D377 + Q449

Q32 + G255
K72 + V264
G133 + V291
T193 + A263
Q256 + T285
D377 + T459

Q32 + Q256
K72 + Y267
G133 + Q299
T193 + V264
Q256 + M286
D377 + P473

Q32 + A263
K72 + N270
G133 + R320
T193 + Y267
Q256 + F289
D377 + C474

Q32 + V264
K72 + G273
G133 + H321
T193 + N270
Q256 + V291
D377 + G476

Q32 + Y267
K72 + S280
G133 + S323
T193 + G273
Q256 + Q299
D377 + G477

Q32 + N270
K72 + T285
G133 + H324
T193 + S280
Q256 + R320
D377 + K484

Q32 + G273
K72 + M286
G133 + F328
T193 + T285
Q256 + H321
D379 + Y382

Q32 + S280
K72 + F289
G133 + T334
T193 + M286
Q256 + S323
D379 + S383

Q32 + T285
K72 + V291
G133 + D337
T193 + F289
Q256 + H324
D379 + Q385

Q32 + M286
K72 + Q299
G133 + Q345
T193 + V291
Q256 + F328
D379 + K391

Q32 + F289
K72 + R320
G133 + G346
T193 + Q299
Q256 + T334
D379 + K393

Q32 + V291
K72 + H321
G133 + G348
T193 + R320
Q256 + D337
D379 + Q395

Q32 + Q299
K72 + S323
G133 + T355
T193 + H321
Q256 + Q345
D379 + A420

Q32 + R320
K72 + H324
G133 + S376
T193 + S323
Q256 + G346
D379 + G423

Q32 + H321
K72 + F328
G133 + D377
T193 + H324
Q256 + G348
D379 + T444

Q32 + S323
K72 + T334
G133 + D379
T193 + F328
Q256 + T355
D379 + A445

Q32 + H324
K72 + D337
G133 + Y382
T193 + T334
Q256 + S376
D379 + Q449

Q32 + F328
K72 + Q345
G133 + S383
T193 + D337
Q256 + D377
D379 + T459

Q32 + T334
K72 + G346
G133 + Q385
T193 + Q345
Q256 + D379
D379 + P473

Q32 + D337
K72 + G348
G133 + K391
T193 + G346
Q256 + Y382
D379 + C474

Q32 + Q345
K72 + T355
G133 + K393
T193 + G348
Q256 + S383
D379 + G476

Q32 + G346
K72 + S376
G133 + Q395
T193 + T355
Q256 + Q385
D379 + G477

Q32 + G348
K72 + D377
G133 + A420
T193 + S376
Q256 + K391
D379 + K484

Q32 + T355
K72 + D379
G133 + G423
T193 + D377
Q256 + K393
Y382 + S383

Q32 + S376
K72 + Y382
G133 + T444
T193 + D379
Q256 + Q395
Y382 + Q385

Q32 + D377
K72 + S383
G133 + A445
T193 + Y382
Q256 + A420
Y382 + K391

Q32 + D379
K72 + Q385
G133 + Q449
T193 + S383
Q256 + G423
Y382 + K393

Q32 + Y382
K72 + K391
G133 + T459
T193 + Q385
Q256 + T444
Y382 + Q395

Q32 + S383
K72 + K393
G133 + P473
T193 + K391
Q256 + A445
Y382 + A420

Q32 + Q385
K72 + Q395
G133 + C474
T193 + K393
Q256 + Q449
Y382 + G423

Q32 + K391
K72 + A420
G133 + G476
T193 + Q395
Q256 + T459
Y382 + T444

Q32 + K393
K72 + G423
G133 + G477
T193 + A420
Q256 + P473
Y382 + A445

Q32 + Q395
K72 + T444
G133 + K484
T193 + G423
Q256 + C474
Y382 + Q449

Q32 + A420
K72 + A445
T134 + W140
T193 + T444
Q256 + G476
Y382 + T459

Q32 + G423
K72 + Q449
T134 + G142
T193 + A445
Q256 + G477
Y382 + P473

Q32 + T444
K72 + T459
T134 + G149
T193 + Q449
Q256 + K484
Y382 + C474

Q32 + A445
K72 + P473
T134 + T165
T193 + T459
A263 + V264
Y382 + G476

Q32 + Q449
K72 + C474
T134 + W167
T193 + P473
A263 + Y267
Y382 + G477

Q32 + T459
K72 + G476
T134 + R171
T193 + C474
A263 + N270
Y382 + K484

Q32 + P473
K72 + G477
T134 + Q172
T193 + G476
A263 + G273
S383 + Q385

Q32 + C474
K72 + K484
T134 + L173
T193 + G477
A263 + S280
S383 + K391

Q32 + G476
R87 + Q98
T134 + A174
T193 + K484
A263 + T285
S383 + K393

Q32 + G477
R87 + M105
T134 + G184
N195 + A204
A263 + M286
S383 + Q395

Q32 + K484
R87 + G109
T134 + T193
N195 + V206
A263 + F289
S383 + A420

A37 + T40
R87 + F113
T134 + N195
N195 + P211
A263 + V291
S383 + G423

A37 + P45
R87 + R116
T134 + A204
N195 + I214
A263 + Q299
S383 + T444

A37 + W48
R87 + Q118
T134 + V206
N195 + V215
A263 + R320
S383 + A445

A37 + G50
R87 + Q125
T134 + P211
N195 + L217
A263 + H321
S383 + Q449

A37 + T51
R87 + G133
T134 + I214
N195 + L219
A263 + S323
S383 + T459

A37 + N54
R87 + T134
T134 + V215
N195 + L235
A263 + H324
S383 + P473

A37 + V56
R87 + W140
T134 + L217
N195 + V238
A263 + F328
S383 + C474

A37 + K72
R87 + G142
T134 + L219
N195 + M246
A263 + T334
S383 + G476

A37 + R87
R87 + G149
T134 + L235
N195 + M248
A263 + D337
S383 + G477

A37 + Q98
R87 + T165
T134 + V238
N195 + L250
A263 + Q345
S383 + K484

A37 + M105
R87 + W167
T134 + M246
N195 + G255
A263 + G346
Q385 + K391

A37 + G109
R87 + R171
T134 + M248
N195 + Q256
A263 + G348
Q385 + K393

A37 + F113
R87 + Q172
T134 + L250
N195 + A263
A263 + T355
Q385 + Q395

A37 + R116
R87 + L173
T134 + G255
N195 + V264
A263 + S376
Q385 + A420

A37 + Q118
R87 + A174
T134 + Q256
N195 + Y267
A263 + D377
Q385 + G423

A37 + Q125
R87 + G184
T134 + A263
N195 + N270
A263 + D379
Q385 + T444

A37 + G133
R87 + T193
T134 + V264
N195 + G273
A263 + Y382
Q385 + A445

A37 + T134
R87 + N195
T134 + Y267
N195 + S280
A263 + S383
Q385 + Q449

A37 + W140
R87 + A204
T134 + N270
N195 + T285
A263 + Q385
Q385 + T459

A37 + G142
R87 + V206
T134 + G273
N195 + M286
A263 + K391
Q385 + P473

A37 + G149
R87 + P211
T134 + S280
N195 + F289
A263 + K393
Q385 + C474

A37 + T165
R87 + I214
T134 + T285
N195 + V291
A263 + Q395
Q385 + G476

A37 + W167
R87 + V215
T134 + M286
N195 + Q299
A263 + A420
Q385 + G477

A37 + R171
R87 + L217
T134 + F289
N195 + R320
A263 + G423
Q385 + K484

A37 + Q172
R87 + L219
T134 + V291
N195 + H321
A263 + T444
K391 + K393

A37 + L173
R87 + L235
T134 + Q299
N195 + S323
A263 + A445
K391 + Q395

A37 + A174
R87 + V238
T134 + R320
N195 + H324
A263 + Q449
K391 + A420

A37 + G184
R87 + M246
T134 + H321
N195 + F328
A263 + T459
K391 + G423

A37 + T193
R87 + M248
T134 + S323
N195 + T334
A263 + P473
K391 + T444

A37 + N195
R87 + L250
T134 + H324
N195 + D337
A263 + C474
K391 + A445

A37 + A204
R87 + G255
T134 + F328
N195 + Q345
A263 + G476
K391 + Q449

A37 + V206
R87 + Q256
T134 + T334
N195 + G346
A263 + G477
K391 + T459

A37 + P211
R87 + A263
T134 + D337
N195 + G348
A263 + K484
K391 + P473

A37 + I214
R87 + V264
T134 + Q345
N195 + T355
V264 + Y267
K391 + C474

A37 + V215
R87 + Y267
T134 + G346
N195 + S376
V264 + N270
K391 + G476

A37 + L217
H1 + Q169
T134 + G348
N195 + D377
V264 + G273
K391 + G477

A37 + L219
H1 + A186
T134 + T355
N195 + D379
V264 + S280
K391 + K484

A37 + L235
H1 + E190
T134 + S376
N195 + Y382
V264 + T285
K393 + Q395

A37 + V238
H1 + A225
T134 + D377
N195 + S383
V264 + M286
K393 + A420

A37 + M246
H1 + K242
T134 + D379
N195 + Q385
V264 + F289
K393 + G423

A37 + M248
H1 + S244
T134 + Y382
N195 + K391
V264 + V291
K393 + T444

A37 + L250
H1 + G258
T134 + S383
N195 + K393
V264 + Q299
K393 + A445

A37 + G255
H1 + N260
T134 + Q385
N195 + Q395
V264 + R320
K393 + Q449

A37 + Q256
H1 + K269
T134 + K391
N195 + A420
V264 + H321
K393 + T459

A37 + A263
H1 + W284
T134 + K393
N195 + G423
V264 + S323
K393 + P473

A37 + V264
H1 + Y295
T134 + Q395
N195 + T444
V264 + H324
K393 + C474

A37 + Y267
H1 + S304
T134 + A420
N195 + A445
V264 + F328
K393 + G476

A37 + N270
H1 + V326
T134 + G423
N195 + Q449
V264 + T334
K393 + G477

A37 + G273
T5 + Q169
T134 + T444
N195 + T459
V264 + D337
K393 + K484

A37 + S280
T5 + A186
T134 + A445
N195 + P473
V264 + Q345
Q395 + A420

A37 + T285
T5 + E190
T134 + Q449
N195 + C474
V264 + G346
Q395 + G423

A37 + M286
T5 + A225
T134 + T459
N195 + G476
V264 + G348
Q395 + T444

A37 + F289
T5 + K242
T134 + P473
N195 + G477
V264 + T355
Q395 + A445

A37 + V291
T5 + S244
T134 + C474
N195 + K484
V264 + S376
Q395 + Q449

A37 + Q299
T5 + G258
T134 + G476
A204 + V206
V264 + D377
Q395 + T459

A37 + R320
T5 + N260
T134 + G477
A204 + P211
V264 + D379
Q395 + P473

A37 + H321
T5 + K269
T134 + K484
A204 + I214
V264 + Y382
Q395 + C474

A37 + S323
T5 + W284
W140 + G142
A204 + V215
V264 + S383
Q395 + G476

A37 + H324
T5 + Y295
W140 + G149
A204 + L217
V264 + Q385
Q395 + G477

A37 + F328
T5 + S304
W140 + T165
A204 + L219
V264 + K391
Q395 + K484

A37 + T334
T5 + V326
W140 + W167
A204 + L235
V264 + K393
A420 + G423

A37 + D337
G7 + Q169
W140 + R171
A204 + V238
V264 + Q395
A420 + T444

A37 + Q345
G7 + A186
W140 + Q172
A204 + M246
V264 + A420
A420 + A445

A37 + G346
G7 + E190
W140 + L173
A204 + M248
V264 + G423
A420 + Q449

A37 + G348
G7 + A225
W140 + A174
A204 + L250
V264 + T444
A420 + T459

A37 + T355
G7 + K242
W140 + G184
A204 + G255
V264 + A445
A420 + P473

A37 + S376
G7 + S244
W140 + T193
A204 + Q256
V264 + Q449
A420 + C474

A37 + D377
G7 + G258
W140 + N195
A204 + A263
V264 + T459
A420 + G476

A37 + D379
G7 + N260
W140 + A204
A204 + V264
V264 + P473
A420 + G477

A37 + Y382
G7 + A265
W140 + V206
A204 + Y267
V264 + C474
A420 + K484

A37 + S383
G7 + K269
W140 + P211
A204 + N270
V264 + G476
G423 + T444

A37 + Q385
G7 + W284
W140 + I214
A204 + G273
V264 + G477
G423 + A445

A37 + K391
G7 + Y295
W140 + V215
A204 + S280
V264 + K484
G423 + Q449

A37 + K393
G7 + S304
W140 + L217
A204 + T285
Y267 + N270
G423 + T459

A37 + Q395
G7 + V326
W140 + L219
A204 + M286
Y267 + G273
G423 + P473

A37 + A420
Q11 + Q169
W140 + L235
A204 + F289
Y267 + S280
G423 + C474

A37 + G423
Q11 + A186
W140 + V238
A204 + V291
Y267 + T285
G423 + G476

A37 + T444
Q11 + E190
W140 + M246
A204 + Q299
Y267 + M286
G423 + G477

A37 + A445
Q11 + A225
W140 + M248
A204 + R320
Y267 + F289
G423 + K484

A37 + Q449
Q11 + K242
W140 + L250
A204 + H321
Y267 + V291
T444 + A445

A37 + T459
Q11 + S244
W140 + G255
A204 + S323
Y267 + Q299
T444 + Q449

A37 + P473
Q11 + G258
W140 + Q256
A204 + H324
Y267 + R320
T444 + T459

A37 + C474
Q11 + N260
W140 + A263
A204 + F328
Y267 + H321
T444 + P473

A37 + G476
Q11 + K269
W140 + V264
A204 + T334
Y267 + S323
T444 + C474

A37 + G477
Q11 + W284
W140 + Y267
A204 + D337
Y267 + H324
T444 + G476

A37 + K484
Q11 + Y295
W140 + N270
A204 + Q345
Y267 + F328
T444 + G477

T40 + P45
Q11 + S304
W140 + G273
A204 + G346
Y267 + T334
T444 + K484

T40 + W48
Q11 + V326
W140 + S280
A204 + G348
Y267 + D337
A445 + Q449

T40 + G50
N16 + Q169
W140 + T285
A204 + T355
Y267 + Q345
A445 + T459

T40 + T51
N16 + A186
W140 + M286
A204 + S376
Y267 + G346
A445 + P473

T40 + N54
N16 + E190
W140 + F289
A204 + D377
Y267 + G348
A445 + C474

T40 + V56
N16 + A225
W140 + V291
A204 + D379
Y267 + T355
A445 + G476

T40 + K72
N16 + K242
W140 + Q299
A204 + Y382
Y267 + S376
A445 + G477

T40 + R87
N16 + S244
W140 + R320
A204 + S383
Y267 + D377
A445 + K484

T40 + Q98
N16 + G258
W140 + H321
A204 + Q385
Y267 + D379
Q449 + T459

T40 + M105
N16 + N260
W140 + S323
A204 + K391
Y267 + Y382
Q449 + P473

T40 + G109
N16 + K269
W140 + H324
A204 + K393
Y267 + S383
Q449 + C474

T40 + F113
N16 + W284
W140 + F328
A204 + Q395
Y267 + Q385
Q449 + G476

T40 + R116
N16 + Y295
W140 + T334
A204 + A420
Y267 + K391
Q449 + G477

T40 + Q118
N16 + S304
W140 + D337
A204 + G423
Y267 + K393
Q449 + K484

T40 + Q125
N16 + V326
W140 + Q345
A204 + T444
Y267 + Q395
T459 + P473

T40 + G133
V17 + Q169
W140 + G346
A204 + A445
Y267 + A420
T459 + C474

T40 + T134
V17 + A186
W140 + G348
A204 + Q449
Y267 + G423
T459 + G476

T40 + W140
V17 + E190
W140 + T355
A204 + T459
Y267 + T444
T459 + G477

T40 + G142
V17 + A225
W140 + S376
A204 + P473
Y267 + A445
T459 + K484

T40 + G149
V17 + K242
W140 + D377
A204 + C474
Y267 + Q449
P473 + C474

T40 + T165
V17 + S244
W140 + D379
A204 + G476
Y267 + T459
P473 + G476

T40 + W167
V17 + G258
W140 + Y382
A204 + G477
Y267 + P473
P473 + G477

T40 + R171
V17 + N260
W140 + S383
A204 + K484
G109 + Q169
P473 + K484

T40 + Q172
V17 + K269
W140 + Q385
V206 + P211
G109 + A186
C474 + G476

T40 + L173
V17 + W284
W140 + K391
V206 + I214
G109 + E190
C474 + G477

T40 + Q169
V17 + Y295
N54 + Q169
K72 + Q169
G109 + A225
C474 + K484

T40 + A186
V17 + S304
N54 + A186
K72 + A186
G109 + K242
G476 + G477

T40 + E190
V17 + V326
N54 + E190
K72 + E190
G109 + S244
G476 + K484

T40 + A225
Q32 + Q169
N54 + A225
K72 + A225
G109 + G258
G477 + K484

T40 + K242
Q32 + A186
N54 + K242
K72 + K242
G109 + N260
G133 + Q169

T40 + S244
Q32 + E190
N54 + S244
K72 + S244
G109 + K269
G133 + A186

T40 + G258
Q32 + A225
N54 + G258
K72 + G258
G109 + W284
G133 + E190

T40 + N260
Q32 + K242
N54 + N260
K72 + N260
G109 + Y295
G133 + A225

T40 + K269
Q32 + S244
N54 + K269
K72 + K269
G109 + S304
G133 + K242

T40 + W284
Q32 + G258
N54 + W284
K72 + W284
G109 + V326
G133 + S244

T40 + Y295
Q32 + N260
N54 + Y295
K72 + Y295
F113 + Q169
G133 + G258

T40 + S304
Q32 + K269
N54 + S304
K72 + S304
F113 + A186
G133 + N260

T40 + V326
Q32 + W284
N54 + V326
K72 + V326
F113 + E190
G133 + K269

P45 + Q169
Q32 + Y295
V56 + Q169
R87 + Q169
F113 + A225
G133 + W284

P45 + A186
Q32 + S304
V56 + A186
R87 + A186
F113 + K242
G133 + Y295

P45 + E190
Q32 + V326
V56 + E190
R87 + E190
F113 + S244
G133 + S304

P45 + A225
A37 + Q169
V56 + A225
R87 + A225
F113 + G258
G133 + V326

P45 + K242
A37 + A186
V56 + K242
R87 + K242
F113 + N260
T134 + Q169

P45 + S244
A37 + E190
V56 + S244
R87 + S244
F113 + K269
T134 + A186

P45 + G258
A37 + A225
V56 + G258
R87 + G258
F113 + W284
T134 + E190

P45 + N260
A37 + K242
V56 + N260
R87 + N260
F113 + Y295
T134 + A225

P45 + K269
A37 + S244
V56 + K269
R87 + K269
F113 + S304
T134 + K242

P45 + W284
A37 + G258
V56 + W284
R87 + W284
F113 + V326
T134 + S244

P45 + Y295
A37 + N260
V56 + Y295
R87 + Y295
R116 + Q169
T134 + G258

P45 + S304
A37 + K269
V56 + S304
R87 + S304
R116 + A186
T134 + N260

P45 + V326
A37 + W284
V56 + V326
R87 + V326
R116 + E190
T134 + K269

W48 + Q169
A37 + Y295
Q169 + R171
Q98 + Q169
R116 + A225
T134 + W284

W48 + A186
A37 + S304
Q169 + Q172
Q98 + A186
R116 + K242
T134 + Y295

W48 + E190
A37 + V326
Q169 + L173
Q98 + E190
R116 + S244
T134 + S304

W48 + A225
G142 + Q169
Q169 + A174
Q98 + A225
R116 + G258
T134 + V326

W48 + K242
G142 + A186
Q169 + G184
Q98 + K242
R116 + N260
W140 + Q169

W48 + S244
G142 + E190
Q169 + A186
Q98 + S244
R116 + K269
W140 + A186

W48 + G258
G142 + A225
Q169 + E190
Q98 + G258
R116 + W284
W140 + E190

W48 + N260
G142 + K242
Q169 + T193
Q98 + N260
R116 + Y295
W140 + A225

W48 + K269
G142 + S244
Q169 + N195
Q98 + K269
R116 + S304
W140 + K242

W48 + W284
G142 + G258
Q169 + A204
Q98 + W284
R116 + V326
W140 + S244

W48 + Y295
G142 + N260
Q169 + V206
Q98 + Y295
Q118 + Q169
W140 + G258

W48 + S304
G142 + K269
Q169 + P211
Q98 + S304
Q118 + A186
W140 + N260

W48 + V326
G142 + W284
Q169 + I214
Q98 + V326
Q118 + E190
W140 + K269

G50 + Q169
G142 + Y295
Q169 + V215
M105 + Q169
Q118 + A225
W140 + W284

G50 + A186
G142 + S304
Q169 + L217
M105 + A186
Q118 + K242
W140 + Y295

G50 + E190
G142 + V326
Q169 + L219
M105 + E190
Q118 + S244
W140 + S304

G50 + A225
G149 + Q169
Q169 + A225
M105 + A225
Q118 + G258
W140 + V326

G50 + K242
G149 + A186
Q169 + L235
M105 + K242
Q118 + N260
E190 + L235

G50 + S244
G149 + E190
Q169 + V238
M105 + S244
Q118 + K269
A225 + V238

G50 + G258
G149 + A225
Q169 + K242
M105 + G258
Q118 + W284
A225 + K242

G50 + N260
G149 + K242
Q169 + S244
M105 + N260
Q118 + Y295
A225 + S244

G50 + K269
G149 + S244
Q169 + M246
M105 + K269
Q118 + S304
A225 + M246

G50 + W284
G149 + G258
Q169 + M248
M105 + W284
Q118 + V326
A225 + M248

G50 + Y295
G149 + N260
Q169 + L250
M105 + Y295
Q125 + Q169
A225 + L250

G50 + S304
G149 + K269
Q169 + G255
M105 + S304
Q125 + A186
A225 + G255

G50 + V326
G149 + W284
Q169 + Q256
M105 + V326
Q125 + E190
A225 + Q256

T51 + Q169
G149 + Y295
Q169 + G258
R171 + A186
Q125 + A225
A225 + G258

T51 + A186
G149 + S304
Q169 + N260
R171 + E190
Q125 + K242
A225 + N260

T51 + E190
G149 + V326
Q169 + A263
R171 + A225
Q125 + S244
A225 + A263

T51 + A225
T165 + Q169
Q169 + V264
R171 + K242
Q125 + G258
A225 + V264

T51 + K242
T165 + A186
Q169 + Y267
R171 + S244
Q125 + N260
A225 + Y267

T51 + S244
T165 + E190
Q169 + K269
R171 + G258
Q125 + K269
A225 + K269

T51 + G258
T165 + A225
Q169 + N270
R171 + N260
Q125 + W284
A225 + N270

T51 + N260
T165 + K242
Q169 + G273
R171 + K269
Q125 + Y295
A225 + G273

T51 + K269
T165 + S244
Q169 + S280
R171 + W284
Q125 + S304
A225 + S280

T51 + W284
T165 + G258
Q169 + W284
R171 + Y295
Q125 + V326
A225 + W284

T51 + Y295
T165 + N260
Q169 + T285
R171 + S304
G184 + A186
A225 + T285

T51 + S304
T165 + K269
Q169 + M286
R171 + V326
G184 + E190
A225 + M286

T51 + V326
T165 + W284
Q169 + F289
Q172 + A186
G184 + A225
A225 + F289

A186 + E190
T165 + Y295
Q169 + V291
Q172 + E190
G184 + K242
A225 + V291

A186 + T193
T165 + S304
Q169 + Y295
Q172 + A225
G184 + S244
A225 + Y295

A186 + N195
T165 + V326
Q169 + Q299
Q172 + K242
G184 + G258
A225 + Q299

A186 + A204
W167 + Q169
Q169 + S304
Q172 + S244
G184 + N260
A225 + S304

A186 + V206
W167 + A186
Q169 + R320
Q172 + G258
G184 + K269
A225 + R320

A186 + P211
W167 + E190
Q169 + H321
Q172 + N260
G184 + W284
A225 + H321

A186 + I214
W167 + A225
Q169 + S323
Q172 + K269
G184 + Y295
A225 + S323

A186 + V215
W167 + K242
Q169 + H324
Q172 + W284
G184 + S304
A225 + H324

A186 + L217
W167 + S244
Q169 + V326
Q172 + Y295
G184 + V326
A225 + V326

A186 + L219
W167 + G258
Q169 + F328
Q172 + S304
L235 + K242
A225 + F328

A186 + A225
W167 + N260
Q169 + T334
Q172 + V326
L235 + S244
A225 + T334

A186 + L235
W167 + K269
Q169 + D337
L173 + A186
L235 + G258
A225 + D337

A186 + V238
W167 + W284
Q169 + Q345
L173 + E190
L235 + N260
A225 + Q345

A186 + K242
W167 + Y295
Q169 + G346
L173 + A225
L235 + K269
A225 + G346

A186 + S244
W167 + S304
Q169 + G348
L173 + K242
L235 + W284
A225 + G348

A186 + M246
W167 + V326
Q169 + T355
L173 + S244
L235 + Y295
A225 + T355

A186 + M248
E190 + T193
Q169 + S376
L173 + G258
L235 + S304
A225 + S376

A186 + L250
E190 + N195
Q169 + D377
L173 + N260
L235 + V326
A225 + D377

A186 + G255
E190 + A204
Q169 + D379
L173 + K269
V238 + K242
A225 + D379

A186 + Q256
E190 + V206
Q169 + Y382
L173 + W284
V238 + S244
A225 + Y382

A186 + G258
E190 + P211
Q169 + S383
L173 + Y295
V238 + G258
A225 + S383

A186 + N260
E190 + I214
Q169 + Q385
L173 + S304
V238 + N260
A225 + Q385

A186 + A263
E190 + V215
Q169 + K391
L173 + V326
V238 + K269
A225 + K391

A186 + V264
E190 + L217
Q169 + K393
A174 + A186
V238 + W284
A225 + K393

A186 + Y267
E190 + L219
Q169 + Q395
A174 + E190
V238 + Y295
A225 + Q395

A186 + K269
E190 + A225
Q169 + A420
A174 + A225
V238 + S304
A225 + A420

A186 + N270
E190 + L235
Q169 + G423
A174 + K242
V238 + V326
A225 + G423

A186 + G273
E190 + V238
Q169 + T444
A174 + S244
K242 + S244
A225 + T444

A186 + S280
E190 + K242
Q169 + A445
A174 + G258
K242 + M246
A225 + A445

A186 + W284
E190 + S244
Q169 + Q449
A174 + N260
K242 + M248
A225 + Q449

A186 + T285
E190 + M246
Q169 + T459
A174 + K269
K242 + L250
A225 + T459

A186 + M286
E190 + M248
Q169 + P473
A174 + W284
K242 + G255
A225 + P473

A186 + F289
E190 + L250
Q169 + C474
A174 + Y295
K242 + Q256
A225 + C474

A186 + V291
E190 + G255
Q169 + G476
A174 + S304
K242 + G258
A225 + G476

A186 + Y295
E190 + Q256
Q169 + G477
A174 + V326
K242 + N260
A225 + G477

A186 + Q299
E190 + G258
Q169 + K484
L217 + A225
K242 + A263
A225 + K484

A186 + S304
E190 + N260
T193 + A225
L217 + K242
K242 + V264
S244 + M248

A186 + R320
E190 + A263
T193 + K242
L217 + S244
K242 + Y267
S244 + L250

A186 + H321
E190 + V264
T193 + S244
L217 + G258
K242 + K269
S244 + G255

A186 + S323
E190 + Y267
T193 + G258
L217 + N260
K242 + N270
S244 + Q256

A186 + H324
E190 + K269
T193 + N260
L217 + K269
K242 + G273
S244 + G258

A186 + V326
E190 + N270
T193 + K269
L217 + W284
K242 + S280
S244 + N260

A186 + F328
E190 + G273
T193 + W284
L217 + Y295
K242 + W284
S244 + A263

A186 + T334
E190 + S280
T193 + Y295
L217 + S304
K242 + T285
S244 + V264

A186 + D337
E190 + W284
T193 + S304
L217 + V326
K242 + M286
S244 + Y267

A186 + Q345
E190 + T285
T193 + V326
L219 + A225
K242 + F289
S244 + K269

A186 + G346
E190 + M286
N195 + A225
L219 + K242
K242 + V291
S244 + N270

A186 + G348
E190 + F289
N195 + K242
L219 + S244
K242 + Y295
S244 + G273

A186 + T355
E190 + V291
N195 + S244
L219 + G258
K242 + Q299
S244 + S280

A186 + S376
E190 + Y295
N195 + G258
L219 + N260
K242 + S304
S244 + W284

A186 + D377
E190 + Q299
N195 + N260
L219 + K269
K242 + R320
S244 + T285

A186 + D379
E190 + S304
N195 + K269
L219 + W284
K242 + H321
S244 + M286

A186 + Y382
E190 + R320
N195 + W284
L219 + Y295
K242 + S323
S244 + F289

A186 + S383
E190 + H321
N195 + Y295
L219 + S304
K242 + H324
S244 + V291

A186 + Q385
E190 + S323
N195 + S304
L219 + V326
K242 + V326
S244 + Y295

A186 + K391
E190 + H324
N195 + V326
M246 + G258
K242 + F328
S244 + Q299

A186 + K393
E190 + V326
A204 + A225
M246 + N260
K242 + T334
S244 + S304

A186 + Q395
E190 + F328
A204 + K242
M246 + K269
K242 + D337
S244 + R320

A186 + A420
E190 + T334
A204 + S244
M246 + W284
K242 + Q345
S244 + H321

A186 + G423
E190 + D337
A204 + G258
M246 + Y295
K242 + G346
S244 + S323

A186 + T444
E190 + Q345
A204 + N260
M246 + S304
K242 + G348
S244 + H324

A186 + A445
E190 + G346
A204 + K269
M246 + V326
K242 + T355
S244 + V326

A186 + Q449
E190 + G348
A204 + W284
M248 + G258
K242 + S376
S244 + F328

A186 + T459
E190 + T355
A204 + Y295
M248 + N260
K242 + D377
S244 + T334

A186 + P473
E190 + S376
A204 + S304
M248 + K269
K242 + D379
S244 + D337

A186 + C474
E190 + D377
A204 + V326
M248 + W284
K242 + Y382
S244 + Q345

A186 + G476
E190 + D379
V206 + A225
M248 + Y295
K242 + S383
S244 + G346

A186 + G477
E190 + Y382
V206 + K242
M248 + S304
K242 + Q385
S244 + G348

A186 + K484
E190 + S383
V206 + S244
M248 + V326
K242 + K391
S244 + T355

I214 + A225
E190 + Q385
V206 + G258
L250 + G258
K242 + K393
S244 + S376

I214 + K242
E190 + K391
V206 + N260
L250 + N260
K242 + Q395
S244 + D377

I214 + S244
E190 + K393
V206 + K269
L250 + K269
K242 + A420
S244 + D379

I214 + G258
E190 + Q395
V206 + W284
L250 + W284
K242 + G423
S244 + Y382

I214 + N260
E190 + A420
V206 + Y295
L250 + Y295
K242 + T444
S244 + S383

I214 + K269
E190 + G423
V206 + S304
L250 + S304
K242 + A445
S244 + Q385

I214 + W284
E190 + T444
V206 + V326
L250 + V326
K242 + Q449
S244 + K391

I214 + Y295
E190 + A445
P211 + A225
G255 + G258
K242 + T459
S244 + K393

I214 + S304
E190 + Q449
P211 + K242
G255 + N260
K242 + P473
S244 + Q395

I214 + V326
E190 + T459
P211 + S244
G255 + K269
K242 + C474
S244 + A420

V215 + A225
E190 + P473
P211 + G258
G255 + W284
K242 + G476
S244 + G423

V215 + K242
E190 + C474
P211 + N260
G255 + Y295
K242 + G477
S244 + T444

V215 + S244
E190 + G476
P211 + K269
G255 + S304
K242 + K484
S244 + A445

V215 + G258
E190 + G477
P211 + W284
G255 + V326
N260 + A263
S244 + Q449

V215 + N260
E190 + K484
P211 + Y295
Q256 + G258
N260 + V264
S244 + T459

V215 + K269
G258 + N260
P211 + S304
Q256 + N260
N260 + Y267
S244 + P473

V215 + W284
G258 + A263
P211 + V326
Q256 + K269
N260 + K269
S244 + C474

V215 + Y295
G258 + V264
A236 + K269
Q256 + W284
N260 + N270
S244 + G476

V215 + S304
G258 + Y267
A236 + W284
Q256 + Y295
N260 + G273
S244 + G477

V215 + V326
G258 + K269
A236 + Y295
Q256 + S304
N260 + S280
S244 + K484

N270 + W284
G258 + N270
A236 + S304
Q256 + V326
N260 + W284
K269 + N270

N270 + Y295
G258 + G273
A236 + V326
W284 + T285
N260 + T285
K269 + G273

N270 + S304
G258 + S280
V264 + K269
W284 + M286
N260 + M286
K269 + S280

N270 + V326
G258 + W284
V264 + W284
W284 + F289
N260 + F289
K269 + W284

G273 + W284
G258 + T285
V264 + Y295
W284 + V291
N260 + V291
K269 + T285

G273 + Y295
G258 + M286
V264 + S304
W284 + Y295
N260 + Y295
K269 + M286

G273 + S304
G258 + F289
V267 + V326
W284 + Q299
N260 + Q299
K269 + F289

G273 + V326
G258 + V291
Y267 + K269
W284 + S304
N260 + S304
K269 + V291

S280 + W284
G258 + Y295
Y267 + W284
W284 + R320
N260 + R320
K269 + Y295

S280 + Y295
G258 + Q299
Y267 + Y295
W284 + H321
N260 + H321
K269 + Q299

S280 + S304
G258 + S304
Y267 + S304
W284 + S323
N260 + S323
K269 + S304

S280 + V326
G258 + R320
Y267 + V326
W284 + H324
N260 + H324
K269 + R320

T285 + Y295
G258 + H321
A288 + F289
W284 + V326
N260 + V326
K269 + H321

T285 + S304
G258 + S323
A288 + V291
W284 + F328
N260 + F328
K269 + S323

T285 + V326
G258 + H324
A288 + Y295
W284 + T334
N260 + T334
K269 + H324

M286 + Y295
G258 + V326
A288 + Q299
W284 + D337
N260 + D337
K269 + V326

M286 + S304
G258 + F328
A288 + S304
W284 + Q345
N260 + Q345
K269 + F328

M286 + V326
G258 + T334
A288 + R320
W284 + G346
N260 + G346
K269 + T334

F289 + Y295
G258 + D337
A288 + H321
W284 + G348
N260 + G348
K269 + D337

F289 + S304
G258 + Q345
A288 + S323
W284 + T355
N260 + T355
K269 + Q345

F289 + V326
G258 + G346
A288 + H324
W284 + S376
N260 + S376
K269 + G346

V291 + Y295
G258 + G348
A288 + V326
W284 + D377
N260 + D377
K269 + G348

V291 + S304
G258 + T355
A288 + F328
W284 + D379
N260 + D379
K269 + T355

V291 + V326
G258 + S376
A288 + T334
W284 + Y382
N260 + Y382
K269 + S376

Y295 + Q299
G258 + D377
A288 + D337
W284 + S383
N260 + S383
K269 + D377

Y295 + S304
G258 + D379
A288 + Q345
W284 + Q385
N260 + Q385
K269 + D379

Y295 + R320
G258 + Y382
A288 + G346
W284 + K391
N260 + K391
K269 + Y382

Y295 + H321
G258 + S383
A288 + G348
W284 + K393
N260 + K393
K269 + S383

Y295 + S323
G258 + Q385
A288 + T355
W284 + Q395
N260 + Q395
K269 + Q385

Y295 + H324
G258 + K391
A288 + S376
W284 + A420
N260 + A420
K269 + K391

Y295 + V326
G258 + K393
A288 + D377
W284 + G423
N260 + G423
K269 + K393

Y295 + F328
G258 + Q395
A288 + D379
W284 + T444
N260 + T444
K269 + Q395

Y295 + T334
G258 + A420
A288 + Y382
W284 + A445
N260 + A445
K269 + A420

Y295 + D337
G258 + G423
A288 + S383
W284 + Q449
N260 + Q449
K269 + G423

Y295 + Q345
G258 + T444
A288 + Q385
W284 + T459
N260 + T459
K269 + T444

Y295 + G346
G258 + A445
A288 + K391
W284 + P473
N260 + P473
K269 + A445

Y295 + G348
G258 + Q449
A288 + K393
W284 + C474
N260 + C474
K269 + Q449

Y295 + T355
G258 + T459
A288 + Q395
W284 + G476
N260 + G476
K269 + T459

Y295 + S376
G258 + P473
A288 + A420
W284 + G477
N260 + G477
K269 + P473

Y295 + D377
G258 + C474
A288 + G423
W284 + K484
N260 + K484
K269 + C474

Y295 + D379
G258 + G476
A288 + T444
S304 + V326
S304 + K393
K269 + G476

Y295 + Y382
G258 + G477
A288 + A445
S304 + Y295
S304 + Q395
K269 + G477

Y295 + S383
G258 + K484
A288 + Q449
S304 + S304
S304 + A420
K269 + K484

Y295 + Q385
Q299 + S304
A288 + T459
S304 + V326
S304 + G423
S304 + T355

Y295 + K391
Q299 + V326
A288 + P473
S304 + Q299
S304 + T444
S304 + S376

Y295 + K393
R320 + V326
A288 + C474
S304 + S304
S304 + A445
S304 + D377

Y295 + Q395
H321 + V326
A288 + G476
S304 + R320
S304 + Q449
S304 + D379

Y295 + A420
S323 + V326
A288 + G477
S304 + H321
S304 + T459
S304 + Y382

Y295 + G423
H324 + V326
A288 + K484
S304 + S323
S304 + P473
S304 + S383

Y295 + T444
Y295 + P473
S304 + Q345
S304 + H324
S304 + C474
S304 + Q385

Y295 + A445
Y295 + C474
S304 + G346
S304 + V326
S304 + G476
S304 + K391

Y295 + Q449
Y295 + G476
S304 + G348
S304 + F328
S304 + G477
S304 + D337

Y295 + T459
Y295 + G477
Y295 + K484
S304 + T334
S304 + K484

wherein numbering is according to SEQ ID NO: 13; or

H1 + A37
N54 + F113
K72 + G109
R116 + A174
T165 + A174
N195 + V206

H1 + T40
N54 + R116
K72 + F113
R116 + G184
T165 + G184
N195 + L228

H1 + N54
N54 + N125
K72 + R116
R116 + N195
T165 + N195
N195 + G255

H1 + V56
N54 + F133
K72 + N125
R116 + G196
T165 + G196
N195 + A265

H1 + A60
N54 + T134
K72 + F133
R116 + A204
T165 + A204
N195 + S280

H1 + K72
N54 + T165
K72 + T134
R116 + V206
T165 + V206
N195 + S304

H1 + G109
N54 + Q172
K72 + T165
R116 + L228
T165 + L228
N195 + R320

H1 + F113
N54 + L173
K72 + Q172
R116 + G255
T165 + G255
N195 + H321

H1 + R116
N54 + A174
K72 + L173
R116 + A265
T165 + A265
N195 + S323

H1 + N125
N54 + G184
K72 + A174
R116 + S280
T165 + S280
N195 + K391

H1 + F133
N54 + N195
K72 + G184
R116 + S304
T165 + S304
G196 + A204

H1 + T134
N54 + G196
K72 + N195
R116 + R320
T165 + R320
G196 + V206

H1 + T165
N54 + A204
K72 + G196
R116 + H321
T165 + H321
G196 + L228

H1 + Q172
N54 + V206
K72 + A204
R116 + S323
T165 + S323
G196 + G255

H1 + L173
N54 + L228
K72 + V206
R116 + K391
T165 + K391
G196 + A265

H1 + A174
N54 + G255
K72 + L228
N125 + F133
Q172 + L173
G196 + S280

H1 + G184
N54 + A265
K72 + G255
N125 + T134
Q172 + A174
G196 + S304

H1 + N195
N54 + S280
K72 + A265
N125 + T165
Q172 + G184
G196 + R320

H1 + G196
N54 + S304
K72 + S280
N125 + Q172
Q172 + N195
G196 + H321

H1 + A204
N54 + R320
K72 + S304
N125 + L173
Q172 + G196
G196 + S323

H1 + V206
N54 + H321
K72 + R320
N125 + A174
Q172 + A204
G196 + K391

H1 + L228
N54 + S323
K72 + H321
N125 + G184
Q172 + V206
A204 + V206

H1 + G255
N54 + K391
K72 + S323
N125 + N195
Q172 + L228
A204 + L228

H1 + A265
V56 + A60
K72 + K391
N125 + G196
Q172 + G255
A204 + G255

H1 + S280
V56 + K72
G109 + F113
N125 + A204
Q172 + A265
A204 + A265

H1 + S304
V56 + G109
G109 + R116
N125 + V206
Q172 + S280
A204 + S280

H1 + R320
V56 + F113
G109 + N125
N125 + L228
Q172 + S304
A204 + S304

H1 + H321
V56 + R116
G109 + F133
N125 + G255
Q172 + R320
A204 + R320

H1 + S323
V56 + N125
G109 + T134
N125 + A265
Q172 + H321
A204 + H321

H1 + K391
V56 + F133
G109 + T165
N125 + S280
Q172 + S323
A204 + S323

A37 + T40
V56 + T134
G109 + Q172
N125 + S304
Q172 + K391
A204 + K391

A37 + N54
V56 + T165
G109 + L173
N125 + R320
L173 + A174
V206 + L228

A37 + V56
V56 + Q172
G109 + A174
N125 + H321
L173 + G184
V206 + G255

A37 + A60
V56 + L173
G109 + G184
N125 + S323
L173 + N195
V206 + A265

A37 + K72
V56 + A174
G109 + N195
N125 + K391
L173 + G196
V206 + S280

A37 + G109
V56 + G184
G109 + G196
F133 + T134
L173 + A204
V206 + S304

A37 + F113
V56 + N195
G109 + A204
F133 + T165
L173 + V206
V206 + R320

A37 + R116
V56 + G196
G109 + V206
F133 + Q172
L173 + L228
V206 + H321

A37 + N125
V56 + A204
G109 + L228
F133 + L173
L173 + G255
V206 + S323

A37 + F133
V56 + V206
G109 + G255
F133 + A174
L173 + A265
V206 + K391

A37 + T134
V56 + L228
G109 + A265
F133 + G184
L173 + S280
L228 + G255

A37 + T165
V56 + G255
G109 + S280
F133 + N195
L173 + S304
L228 + A265

A37 + Q172
V56 + A265
G109 + S304
F133 + G196
L173 + R320
L228 + S280

A37 + L173
V56 + S280
G109 + R320
F133 + A204
L173 + H321
L228 + S304

A37 + A174
V56 + S304
G109 + H321
F133 + V206
L173 + S323
L228 + R320

A37 + G184
V56 + R320
G109 + S323
F133 + L228
L173 + K391
L228 + H321

A37 + N195
V56 + H321
G109 + K391
F133 + G255
A174 + G184
L228 + S323

A37 + G196
V56 + S323
F113 + R116
F133 + A265
A174 + N195
L228 + K391

A37 + A204
V56 + K391
F113 + N125
F133 + S280
A174 + G196
G255 + A265

A37 + V206
A60 + K72
F113 + F133
F133 + S304
A174 + A204
G255 + S280

A37 + L228
A60 + G109
F113 + T134
F133 + R320
A174 + V206
G255 + S304

A37 + G255
A60 + F113
F113 + T165
F133 + H321
A174 + L228
G255 + R320

A37 + A265
A60 + R116
F113 + Q172
F133 + S323
A174 + G255
G255 + H321

A37 + S280
A60 + N125
F113 + L173
F133 + K391
A174 + A265
G255 + S323

A37 + S304
A60 + F133
F113 + A174
T134 + T165
A174 + S280
G255 + K391

A37 + R320
A60 + T134
F113 + G184
T134 + Q172
A174 + S304
A265 + S280

A37 + H321
A60 + T165
F113 + N195
T134 + L173
A174 + R320
A265 + S304

A37 + S323
A60 + Q172
F113 + G196
T134 + A174
A174 + H321
A265 + R320

A37 + K391
A60 + L173
F113 + A204
T134 + G184
A174 + S323
A265 + H321

T40 + N54
A60 + A174
F113 + V206
T134 + N195
A174 + K391
A265 + S323

T40 + V56
A60 + G184
F113 + L228
T134 + G196
G184 + N195
A265 + K391

T40 + A60
A60 + N195
F113 + G255
T134 + A204
G184 + G196
S280 + S304

T40 + K72
A60 + G196
F113 + A265
T134 + V206
G184 + A204
S280 + R320

T40 + G109
A60 + A204
F113 + S280
T134 + L228
G184 + V206
S280 + H321

T40 + F113
A60 + V206
F113 + S304
T134 + G255
G184 + L228
S280 + S323

T40 + R116
A60 + L228
F113 + R320
T134 + A265
G184 + G255
S280 + K391

T40 + N125
A60 + G255
F113 + H321
T134 + S280
G184 + A265
S304 + R320

T40 + F133
A60 + A265
F113 + S323
T134 + S304
G184 + S280
S304 + H321

T40 + T134
A60 + S280
F113 + K391
T134 + R320
G184 + S304
S304 + S323

T40 + T165
A60 + S304
R116 + N125
T134 + H321
G184 + R320
S304 + K391

T40 + Q172
A60 + R320
R116 + F133
T134 + S323
G184 + H321
R320 + H321

T40 + L173
A60 + H321
R116 + T134
T134 + K391
G184 + S323
R320 + S323

T40 + A174
A60 + S323
R116 + T165
T165 + Q172
G184 + K391
R320 + K391

T40 + G184
A60 + K391
R116 + Q172
T165 + L173
N195 + G196
H321 + S323

T40 + N195
T40 + S323
R116 + L173
H1 + Q169
N195 + A204
H321 + K391

T40 + G196
T40 + K391
T40 + A265
H1 + A186
S244 + G255
S323 + K391

T40 + A204
N54 + V56
T40 + S280
H1 + E190
S244 + G258
A265 + K269

T40 + V206
N54 + A60
T40 + S304
H1 + A225
S244 + N260
A265 + W284

T40 + L228
N54 + K72
T40 + R320
H1 + K242
S244 + A265
A265 + Y295

T40 + G255
N54 + G109
T40 + H321
H1 + S244
S244 + K269
A265 + S304

T40 + Q169
R116 + Q169
A204 + A225
H1 + G258
S244 + S280
A265 + V326

T40 + A186
R116 + A186
A204 + K242
H1 + N260
S244 + W284
K269 + S280

T40 + E190
R116 + E190
A204 + S244
H1 + K269
S244 + Y295
K269 + W284

T40 + A225
R116 + A225
A204 + G258
H1 + W284
S244 + S304
K269 + Y295

T40 + K242
R116 + K242
A204 + N260
H1 + Y295
S244 + R320
K269 + S304

T40 + S244
R116 + S244
A204 + K269
H1 + S304
S244 + H321
K269 + R320

T40 + G258
R116 + G258
A204 + W284
H1 + V326
S244 + S323
K269 + H321

T40 + N260
R116 + N260
A204 + Y295
A37 + Q169
S244 + V326
K269 + S323

T40 + K269
R116 + K269
A204 + S304
A37 + A186
S244 + K391
K269 + V326

T40 + W284
R116 + W284
V206 + A225
A37 + E190
S244 + I405
K269 + K391

T40 + Y295
R116 + Y295
V206 + K242
A37 + A225
S244 + A421
K269 + I405

T40 + S304
R116 + S304
V206 + S244
A37 + K242
S244 + A422
K269 + A421

T40 + V326
N125 + Q169
V206 + G258
A37 + S244
S244 + A428
K269 + A422

N54 + Q169
N125 + A186
V206 + N260
A37 + G258
S244 + G448
K269 + A428

N54 + A186
N125 + E190
V206 + K269
A37 + N260
S244 + D476
K269 + G448

N54 + E190
N125 + A225
V206 + W284
A37 + K269
S244 + G477
K269 + D476

N54 + A225
N125 + K242
V206 + Y295
A37 + W284
G255 + G258
K269 + G477

N54 + K242
N125 + S244
V206 + S304
A37 + Y295
G255 + N260
S280 + W284

N54 + S244
N125 + G258
V206 + V326
A37 + S304
G255 + K269
S280 + Y295

N54 + G258
N125 + N260
A225 + L228
A37 + V326
G255 + W284
S280 + S304

N54 + N260
N125 + K269
A225 + K242
Q172 + A186
G255 + Y295
S280 + V326

N54 + K269
N125 + W284
A225 + S244
Q172 + E190
G255 + S304
W284 + Y295

N54 + W284
N125 + Y295
A225 + G255
Q172 + A225
G255 + V326
W284 + S304

N54 + Y295
N125 + S304
A225 + G258
Q172 + K242
G258 + N260
W284 + R320

N54 + S304
F133 + Q169
A225 + N260
Q172 + S244
G258 + A265
W284 + H321

N54 + V326
F133 + A186
A225 + A265
Q172 + G258
G258 + K269
W284 + S323

V56 + Q169
F133 + E190
A225 + K269
Q172 + N260
G258 + S280
W284 + V326

V56 + A186
F133 + A225
A225 + S280
Q172 + K269
G258 + W284
W284 + K391

V56 + E190
F133 + K242
A225 + W284
Q172 + W284
G258 + Y295
W284 + I405

V56 + A225
F133 + S244
A225 + Y295
Q172 + Y295
G258 + S304
W284 + A421

V56 + K242
F133 + G258
A225 + S304
Q172 + S304
G258 + R320
W284 + A422

V56 + S244
F133 + N260
A225 + R320
L173 + A186
G258 + H321
W284 + A428

V56 + G258
F133 + K269
A225 + H321
L173 + E190
G258 + S323
W284 + G448

V56 + N260
F133 + W284
A225 + S323
L173 + A225
G258 + V326
W284 + D476

V56 + K269
F133 + Y295
A225 + V326
L173 + K242
G258 + K391
W284 + G477

V56 + W284
F133 + S304
A225 + K391
L173 + S244
G258 + I405
Y295 + S304

V56 + Y295
T134 + Q169
A225 + I405
L173 + G258
G258 + A421
Y295 + R320

V56 + S304
T134 + A186
A225 + A421
L173 + N260
G258 + A422
Y295 + H321

V56 + V326
T134 + E190
A225 + A422
L173 + K269
G258 + A428
Y295 + S323

A60 + Q169
T134 + A225
A225 + A428
L173 + W284
G258 + G448
Y295 + V326

A60 + A186
T134 + K242
A225 + G448
L173 + Y295
G258 + D476
Y295 + K391

A60 + E190
T134 + S244
A225 + D476
L173 + S304
G258 + G477
Y295 + I405

A60 + A225
T134 + G258
A225 + G477
A174 + A186
N260 + A265
Y295 + A421

A60 + K242
T134 + N260
L228 + K242
A174 + E190
N260 + K269
Y295 + A422

A60 + S244
T134 + K269
L228 + S244
A174 + A225
N260 + S280
Y295 + A428

A60 + G258
T134 + W284
L228 + G258
A174 + K242
N260 + W284
Y295 + G448

A60 + N260
T134 + Y295
L228 + N260
A174 + S244
N260 + Y295
Y295 + D476

A60 + K269
T134 + S304
L228 + K269
A174 + G258
N260 + S304
Y295 + G477

A60 + W284
T134 + V326
L228 + W284
A174 + N260
N260 + R320
S304 + R320

A60 + Y295
T165 + Q169
L228 + Y295
A174 + K269
N260 + H321
S304 + H321

A60 + S304
T165 + A186
L228 + S304
A174 + W284
N260 + S323
S304 + S323

A60 + V326
T165 + E190
L228 + V326
A174 + Y295
N260 + V326
S304 + V326

K72 + Q169
T165 + N195
K242 + S244
A174 + S304
N260 + K391
S304 + K391

K72 + A186
T165 + A225
K242 + G255
G184 + A186
N260 + I405
S304 + I405

K72 + E190
T165 + K242
K242 + G258
G184 + E190
N260 + A421
S304 + A421

K72 + A225
T165 + S244
K242 + N260
G184 + A225
N260 + A422
S304 + A422

K72 + K242
T165 + G258
K242 + A265
G184 + K242
N260 + A428
S304 + A428

K72 + S244
T165 + N260
K242 + K269
G184 + S244
N260 + G448
S304 + G448

K72 + G258
T165 + K269
K242 + S280
G184 + G258
N260 + D476
S304 + D476

K72 + N260
T165 + W284
K242 + W284
G184 + N260
N260 + G477
S304 + G477

K72 + K269
T165 + Y295
K242 + Y295
G184 + K269
N195 + A225
R320 + H321

K72 + W284
T165 + S304
K242 + S304
G184 + W284
N195 + K242
R320 + V326

K72 + Y295
Q169 + Q172
K242 + R320
G184 + Y295
N195 + S244
H321 + V326

K72 + S304
Q169 + L173
K242 + H321
G184 + S304
N195 + G258
S323 + V326

G109 + Q169
Q169 + A174
K242 + S323
A186 + E190
N195 + N260
V326 + K391

G109 + A186
Q169 + G184
K242 + V326
A186 + N195
N195 + K269
V326 + I405

G109 + E190
Q169 + A186
K242 + K391
A186 + G196
N195 + W284
V326 + A421

G109 + A225
Q169 + E190
K242 + I405
A186 + A204
N195 + Y295
V326 + A422

G109 + K242
Q169 + N195
K242 + A421
A186 + V206
N195 + S304
V326 + A428

G109 + S244
Q169 + G196
K242 + A422
A186 + A225
N195 + V326
V326 + G448

G109 + G258
Q169 + A204
K242 + A428
A186 + L228
G196 + A225
V326 + D476

G109 + N260
Q169 + V206
K242 + G448
A186 + K242
G196 + K242
V326 + G477

G109 + K269
Q169 + A225
K242 + D476
A186 + S244
G196 + S244
E190 + Y295

G109 + W284
Q169 + L228
K242 + G477
A186 + G255
G196 + G258
E190 + S304

G109 + Y295
Q169 + K242
E190 + A204
A186 + G258
G196 + N260
E190 + R320

G109 + S304
Q169 + S244
E190 + V206
A186 + N260
G196 + K269
E190 + H321

G109 + V326
Q169 + G255
E190 + A225
A186 + A265
G196 + W284
E190 + S323

F113 + Q169
Q169 + G258
E190 + L228
A186 + K269
G196 + Y295
E190 + V326

F113 + A186
Q169 + N260
E190 + K242
A186 + S280
G196 + S304
E190 + K391

F113 + E190
Q169 + A265
E190 + S244
A186 + W284
G196 + V326
E190 + I405

F113 + A225
Q169 + K269
E190 + G255
A186 + Y295
A186 + G448
E190 + A421

F113 + K242
Q169 + S280
E190 + G258
A186 + S304
A186 + D476
E190 + A422

F113 + S244
Q169 + W284
E190 + N260
A186 + R320
A186 + G477
E190 + A428

F113 + G258
Q169 + Y295
E190 + A265
A186 + H321
E190 + N195
E190 + G448

F113 + N260
Q169 + S304
E190 + K269
A186 + S323
E190 + G196
E190 + D476

F113 + K269
Q169 + R320
E190 + S280
A186 + V326
Q169 + A422
E190 + G477

F113 + W284
Q169 + H321
E190 + W284
A186 + K391
Q169 + A428
A186 + A422

F113 + Y295
Q169 + S323
Q169 + I405
A186 + I405
Q169 + G448
A186 + A428

F113 + S304
Q169 + V326
Q169 + A421
A186 + A421
Q169 + D476

F113 + V326
Q169 + K391

Q169 + G477

wherein numbering is according to SEQ ID NO: 14.

Stability of a parent polypeptide may be improved by introducing a pairwise deletion within the B domain of parent polypeptide. Thus, in one embodiment, the said variant comprises

a) a deletion and/or a substitution at two or more positions corresponding to positions R181, G182, D183, and G184 of the amino acid sequence as set forth in SEQ ID NOs: 7 or 10, and

b) an alteration at one or more positions corresponding to positions H1, T5, G7, Q11, N16, V17, Q32, A37, T40, P45, W48, G50, T51, N54, V56, K72, R87, Q98, M105, G109, F113, R116, Q118, Q125, G133, T134, W140, G142, G149, T165, W167, Q169, R171, Q172, L173, A174, G184, A186, E190, T193, N195, A204, V206, P211, I214, V215, L217, L219, A225, L235, V238, K242, S244, M246, M248, L250, G255, Q256, N260, A263, V264, Y267, K269, N270, G273, S280, W284, T285, M286, F289, V291, Y295, Q299, R320, H321, S323, H324, V326, F328, T334, D337, Q345, G346, G348, T355, S376, D377, D379, Y382, S383, Q385, K391, K393, Q395, A420, G423, T444, A445, Q449, T459, P473, C474, G476, G477, and K484 of SEQ ID NO: 13; or H1, A37, T40, N54, V56, A60, K72, G109, F113, R116, N125, F133, T134, T165, Q172, L173, A174, G184, N195, G196, A204, V206, L228, G255, A265, S280, S304, R320, H321, S323, K391, I405, A421, A422, A428, R439, G448, W467, D476, and G477 of SEQ ID NO: 14.

The term “pairwise deletion” as used herein, refers to a deletion of an amino acid in two positions. Said two positions may be adjacent to one another but may also be separated by one, two, three, four, or five amino acids.

In a particular embodiment, the deletion a) is selected from the group consting of R181+G182, R181+D183, R181+G184, G182+D183, G182+G184, or D183+G184.

In an embodiment, variant comprises one or more of the following alterations; H1*, T5K, G7K, G7A, Q11H, N16S, N16H, V17L, Q32S, A37H, A37M, A37V, A37S, A37Y, A37R, A37L, T40G, T40K, P45A, W48G, W48Y, W48F, G50A, T51K, T51E, T51G, T51A, T51S, T51G, T51D, N54S, V56T, K72R, K72H, K72S, K72Q, K72E, K72N, K72A, K72M, R87S, Q98S, Q98A, M105F, M105I, M105V, M105L, G109A, G109S, F113W, F113S, F113N, F113Y, F113R, F113L, F113Q, R116Q, R116V, R116K, R116W, R116L, R116A, R116H, R116M, R116E, R116S, R116I, R116G, Q118N, Q118K, Q118G, Q118S, Q118F, Q118R, Q125P, Q125K, Q125A, Q125T, G133S, T134E, W140Y, G142T, G149Q, T165S, T165G, T165V, W167I, W167G, W167F, W167R, W167S, W167H, W167L, W167M, W167Y, Q169E, R171H, Q172G, Q172R, Q172N, Q172D, Q172Y, Q172M, Q172S, Q172T, Q172K, Q172H, Q172E, L173V, L173G, L173H, L173A, L173I, L173P, L173T, L173F, L173M, A174S, A174D, A174P, A174M, A174T, A174H, A174K, A174G, A174Q, A174N, A174V, A174L, G184T, A186D, A186N, A186E, A186Q, A186H, E190P, T193K, N195F, A204T, A204V, A204S, A204G, V206L, V206S, V206Y, P211D, I214G, I214H, I214S, I214T, I214L, I214E, I214W, V215T, L217T, L217Q, L219V, L219H, A225V, L235V, L235A, V238A, V238T, V238G, K242Q, S244Q, M246L, M246A, M246I, M246F, M246V, M246S, M248T, L250I, L250V, L250T, L250A, L250F, L250M, G255N, G255A, G255S, Q256A, N260G, A263G, V264I, V264T, Y2671, Y267M, Y267H, Y267L, K269Q, N270G, N270T, G273R, S280W, S280L, S280T, S280A, 5280K, S280Q, W284H, T285L, T285Q, M286F, M286L, A288L, A288V, F289I, F289L, V291G, V291A, V291T, Y295F, Y295N, Q299V, S304R, S304N, R320A, R320V, H321Y, S323N, H324L, V326L, F328L, F328M, F328V, F328I, T334S, D337H, Q345D, G346T, G346D, G346P, G348S, G348P, T355L, T355F, S376H, S376T, S376V, D377H, D377S, D377A, D377Q, D379S, D379G, D379R, D379A, Y382M, Y382I, Y382L, Y382F, S383G, S383A, Q385L, K391A, K391Y, K391V, K391M, K391E, K391D, K391R, K391H, K391W, K391I, K391Q, K391L, K393Y, K393R, K393Q, K393S, Q395P, T400P, H402R, A420Q, A420S, A420K, A420L, G423H, T444Q, T444S, T444D, T444Y, T444H, T444V, T444R, T444A, A445Q, Q449T, T459N, P473R, P473A, P473G, P473T, P473K, C474V, G476K, G477A, G477Q, K484A, K484G, K484P, K484E, K484Q, and K484S of SEQ ID NO: 13; or H1*, G7A, A37H, T40D, W48Y, W48F, N54S, V56T, A60V, K72R, Q98A, G109A, F113Q, R116H, R116V, R116Q, N125D, F133Q, T134E, T165G, Q169E, Q172D, Q172G, Q172N, L173V, A174S, G184T, A186D, A186N, A186E, A186Q, A186H, E190P, N195F, G196R, A204G, V206L, V206Y, A225V, L2281, K242Q, S244Q, G255A, G255S, N260G, A265G, K269Q, N270T, S280Q, W284H, A288L, A288V, F289L, Y295F, Y295N, S304N, S304R, S304N, R320A, H321Y, S323N, V326L, K391A, I405L, A421H, A422P, A428T, G448D, D476K, D476G, D476N, D476Y, G477S, G477A, G477T, and G477Q of SEQ ID NO: 14.

In a particular embodiment, the variant has an IF of >1 when compared to said parent polypeptide, and wherein said variant is selected from G182*+D183*+N195F H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+R87S+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+T40G+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+T51K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+K72H+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+A37H+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+A37M+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+A37V+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+A37S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+A37Y+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+A37R+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+F113W+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+F113S+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+F113N+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+F113Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+F113R+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+Q118N+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+R116V+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+R116K+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+R116W+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+R116L+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+G142T+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+Q125P+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+Q125K+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S381G+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246T+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V213T+K391A+G476K H1*+A37L+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+Q118K+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+R116A+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+R116H+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+Q125A+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377S+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377G+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377R+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377A+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214G+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S381A+K391A+G476K H1*+N54S+V56T+G109A+W167I+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+W167G+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346S+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346P+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214H+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214S+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214T+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420Q+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420S+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420K+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420L+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391Y+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391R+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473G+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473T+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+Q395P+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444S+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444D+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444Y+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280W+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280L+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280T+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280A+K391A+G476K 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H1*+N54S+V56T+G109A+A174F+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+R116G+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+A174T+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K H1*+N54S+V56T+K72R+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K H1*+T51G+N54S+V56T+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+T51A+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+T51S+N54S+V56T+G109A+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K

H1*+T51A+N54S+V56T+G109A+Q172S+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+T51E+N54S+V56T+G109A+Q172T+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+T51A+N54S+V56T+G109A+Q172A+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+T51S+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+T51G+N54S+V56T+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+T51A+N54S+V56T+G109A+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+T51E+N54S+V56T+G109A+Q172L+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+T51E+N54S+V56T+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+T51D+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+T51A+N54S+V56T+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+K72R+G109A+Q172S+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+K72H+G109A+Q172T+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+K72R+G109A+Q172R+A174S+K179E+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+K72R+G109A+Q172M+A174S+G182*+D183*+N195F+V206L+Y295N+K391A+G476K H1*+N54S+V56T+K72R+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+K72Q+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+K72R+G109A+Q172T+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+K72M+G109A+Q172S+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+K72Q+G109A+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+K72S+G109A+A174H+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+K72Q+G109A+A174D+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+K72R+G109A+A174D+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+K72Q+G109A+A174K+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+A174G+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+K72R+G109A+A174Q+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+K72Q+G109A+A174G+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+K72S+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+K72R+G109A+A174H+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391S+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444A+G476K H1*+N54S+V56T+G109A+F113L+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+G50A+N54S+V56T+G109A+A174E+G182*+D183*+N195F+A204T+V206L+S323N+K391A+G476K H1*+N54S+V56T+K72R+G109A+Q118R+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K H1*+N54S+V56T+M105F+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K H1*+N54S+V56T+K72R+G109A+Q118R+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A225F+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246A+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y3821+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S376H+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214E+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214W+K391A+G476K H1*+N54S+V56T+K72R+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204T+V206L+D377H+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204S+V206L+D377H+K391A+G476K H1*+N54S+V56T+G109A+T165S+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T334S+D377H+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A263G+D377H+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M286F+D377H+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y2671+D377H+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L235V+D377H+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L235A+D377H+K391A+G476K H1*+N54S+V56T+M105L+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K H1*+N54S+V56T+M105I+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K H1*+N54S+V56T+M105V+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246I+D377H+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250I+D377H+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250V+D377H+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391Y+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391V+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391M+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391E+G476K H1*+N54S+V56T+G109A+T165G+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K H1*+N54R+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54L+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109S+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+A174V+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246F+D377H+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246V+D377H+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246S+D377H+K391A+G476K H1*+N54S+V56T+G109A+A174R+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+A174L+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206S+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391D+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391R+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391H+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391W+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K3911+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250T+D377H+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250A+D377H+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250F+D377H+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250M+D377H+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391Q+G476K H1*+N54S+V56T+G109A+Q172H+L173A+A174P+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+Q172E+L173A+A174R+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+K72Q+G109A+W167R+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+G184T+N195F+V206L+D377H+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+G255A+D377H+K391A+G476K H1*+N54S+V56T+G109A+Q118R+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S376T+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S376V+K391A+G476K H1*+N54S+V56T+G109A+Q172E+L173I+A174N+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+Q172D+L173A+A174T+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267M+D377H+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267H+D377H+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238A+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238T+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238G +K391A+G476K H1*+N54S+V56T+M105V+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V264T+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V2641+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L235V+K391A+G476K H1*+N54S+V56T+G109A+W167H+Q172N+L173A+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+W167F+Q172E+L173P+A174K+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+Q172N+L173T+A174S+G182*+D183*+N195F+V206L+K391A+G476K 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N54S+V56T+G109A+A174S+G182*+D183*+N195F+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+K391A H1*+N54S+V56T+G109A+F113Q+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K H1*+N54S+V56T+K72R+G109A+F113Q+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K H1*+N54S+V56T+K72R+G109A+F113Q+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+P473R+G476K H1*+N54S+V56T+K72R+G109A+R116Q+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K H1*+A37V+N54S+V56T+G109A+R116A+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+A37V+N54S+V56T+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+M246F+K391A+G476K H1*+V56A+G109A+A174S+G182*+D183*+N195F+V206L+G476K H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+K391A H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+G476K G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K N54S+V56T+G109A+G182*+D183*+N195F+V206L+K391T+G476K H1*+N54S+V56T+K72R+G109A+F113Q+R116H+A174S+G182*+D183*+G184T+N195F+V206L+K391A+T444Q+G476K H1*+N54S+V56T+G109S+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K H1*+N54S+V56T+G109A+F113Q+R116Q+Q172N+A174S+G182*+D183*+N195F+V206L+A265G+K391A+P473R+G476K H1*+N54S+V56T+G109A+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K H1*+N54S+V56T+G109A+R116A+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K H1*+N54S+V56T+G109A+R116H+Q172R+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K H1*+A37V+N54S+V56T+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+A37H+N54S+V56T+G109A+R116H+T165G+A174S+G182*+D183*+N195F+V206L+M246F+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+K391A+G476K H1*+G109A+G182*+D183*+N195F+V206L+K391A+G476K G109A+G182*+D183*+N195F+V206L+G476K H1*+G109A+G182*+D183*+N195F+K391A H1*+N54S+V56T+G109A+G133S+A174S+G182*+D183*+N195F+K391A+G476H H1*+N54S+V56T+G109A+R116A+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K

H1*+A37V+N54S+V56T+G109A+R116H+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+P45A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K H1*+N54S+V56T+G109A+P124A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+P364A+K391A+C474V+G476K H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K H1*+G50A+T51A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V264F+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y382L+K391A+G476K H1*+G7K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255A+Q256A+K391A+G476K H1*+N54S+V56T+G109A+W167Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+W167H+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+T165V+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+A174Q+G182*+D183*+N195F+V206L+K391A+G476K H1*+N16H+V17L+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y382F+K391A+G476K H1*+Q32S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Q385L+K391A+G476K H1*+N54S+V56T+R87S+G109A+R171H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116H+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+P473R+G476K, H1*+N54S+V56T+G109A+Q169E+Q172G+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+K391A+P473R+G476K, H1*+N54S+V56T+G109A+R116W+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206F+K391A+G476K, H1*+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N260G+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+Y295N+K391A+G476K, H1*+N54S+V56T+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+A186N+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+A288V+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+W48F+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+V291A+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+R320A+S323N+K391A+G476K, H1*+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116H+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N270T+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+N260G+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+A186D+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+A225V+K391A+G476K, H1*+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G255S+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K269Q+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255S+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, G182*+D183*+N195F, H1*+N54S+V56T+G109A+Q169E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+W284H+K391A+G476K, H1*+N54S+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+S304R+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246V+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+N270G+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+Y295F+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174T+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W140Y+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+F328L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G476K, F113Q+R116H+D183*+G182*+N195F, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A288L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N270G+K391A+G476K, Q169E+Q172K+A174*+D183*+G182*+N195F, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K269Q+K391A+G476K, H1*+W48F+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, F113Q+R116Q+D183*+G182*+N195F, N54S+V56T+D183*+G182*+N195F, H1*+N54S+V56T+G109A+A174T+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116W+A174S+G182*+D183*+N195F+V206L+K391A+G476K, Q169E+Q172K+G182*+D183*+N195F, G182*+D183*+A186D, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238A+K391A+G476K, G182*+D183*+A186N, H1*+N54S+V56T+G109A+A174S+V206L+G182*+D183*+K391A+G476K, H1*+N54S+V56T+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206F+K391A+G476K, H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+A186N+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F289L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y295F+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S244Q+K391A+G476K, G182*+D183*+F328L, G182*+D183*+N195F+A288V, G182*+D183*+A186N+N195F, G182*+D183*+E190P+N195F, W140Y+G182*+D183*+N195F, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A225V+K391A+G476K, G182*+D183*+A186D+N195F, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+W48Y+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, G182*+D183*+N195F+V238A, G182*+D183*+Y295N, Q172G+G182*+D183*, F113Q+G182*+D183*+N195F, H1*+N54S+V56T+Q98A+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, G182*+D183*+N195F+Y295F, G182*+D183*+A186E, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206Y+K391A+G476K, G182*+D183*+N195F+W284H, G182*+D183*+A186Q+N195F, K72R+G182*+D183*, D183*+G182*+N195F+V326L, D183*+G182*+A186E+N195F, Q169E+G182*+D183*+N195F, D183*+G182*+N195F+K242Q, G7A+G182*+D183*+N195F, D183*+G182*+N195F+N260G, D183*+G182*+R321A+S323N, D183*+G182*+W284H, H1*+D183*+G182*, D183*+G182*+A186H+N195F, D183*+G182*+F289V, D183*+G182*+K391A, D183*+G182*+S304R, W167F+G182*+D183*+N195F, F113Q+R116H+D183*+G182*, D183*+G182*+N270G, D183*+G182*+S244Q, H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, D183*+G182*+N195F+Y295N, W48F+D183*+G182*, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, D183*+G182*+A186Q, W140Y+D183*+G182*, D183*+G182*+K242Q, D183*+G182*+V206L, D183*+G182*+N195F+V206F, Q169E+D183*+G182*, D183*+G182*+N195F+R320A, G182*+D183*+G184T+N195F, D183*+G182*+G184T, N54S+V56T+D183*+G182*, D183*+G182*+P473R, W48Y+D183*+G182*, D183*+G182*+R323A, G7A+D183*+G182*, G109A+D183*+G182*, W167F+D183*+G182*, H1*+N54S+V56T+G109A+Q169E+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, A174S+D183*+G182*, R116Q+D183*+G182*, D183*+G182*+N195F+V291A, Q172K+D183*+G182*, N54S+D183*+G182*, D183*+G182*+N195F+S323N, D183*+G182*+N195F+M246V, W48Y+D183*+G182*+N195F, D183*+G182*+N195F+S304R,

V56T+D183*+G182*,wherein numbering is according to SEQ ID NO: 13; and N54S+G182*+D183*+N195F, G109A+G182*+D183*+N195F, G182*+D183*+N195F+K391A, G182*+D183*+N195F+G476K, H1*+G182*+D183*+N195F, N54S+G182*+D183*+N195F, V56T+G182*+D183*+N195F, G182*+D183*+N195F+I405L, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T+D476K, A174S+G182*+D183*+N195F, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+A174S+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476G, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476K+G477S, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476K, H1*+N54S+V56T+K72R+G109A+W167F+A174S+G182*+D183*+N195F+V206L+I405L+A421+A422P+A428T, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S304N+I405L+A421H+A422P+A428T+D476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476K+G477A, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476G+G477T, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+G477Q, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+G477A, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476K+G477Q, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476N, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476Y, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206I+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+A204G+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+A204G+V206L+G255A+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+R116H+T165G+Q172G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+Q172N+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+G196R+V206L+G255A+I405L+A421H+A422P+A428T+N475S, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+T40D+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+N125D+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+A60V+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+R116V+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+R116Q+G133Q+T165G+Q172G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+R116Q+Q172G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T+G448D, H1*+N54S+V56T+G109A+F113Q+R116H+Q172N+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+T134E+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+G255A+A265G+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+R116Q+W167F+Q172R+L173V+A174S+G182*+D183*+N195F+V206L+A265G+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+F113Q+T134E+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+G255A+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+G184T+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+G255A+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+R116Q+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+F113Q+F116H+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+N195F+A204G+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+A265G+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+A37H+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+A174S+M105F+G182*+D183*+N195F+V206L+L2281+R320A+S323N+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+F113Q+T134E+A174S+G182*+D183*+N195F+V206L+G255A+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+R116Q+V120L+A174S+G182*+D183*+G184T+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+R116Q+Q172N+A174S+G182*+D183*+N195F+V206L+A265G+I405L+A421H+A422P+A428T, H1*+A37H+N54S+V56T+A60V+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, wherein numbering is according to SEQ ID NO:14.

As can be seen from the data obtained in Examples 3 and 4, all tested variants have an Improvement Factor (IF) of at least 1.0, i.e. at least on par with a parent polypeptide having SEQ ID NO: 15, when tested in either (i) a detergent Model A at 15° C. and an enzyme concentration of 0.05 mg/L, (ii) a detergent Model A at 15° C. and an enzyme concentration of 0.2 mg/L, (iii) a detergent Model J at 15° C. and an enzyme concentration of 0.05 mg/L, or (iv) a detergent Model J at 15° C. and an enzyme concentration of 0.2 mg/L. Thus, the variants of the present invention have even in different model detergent compositions, at a low temperature as well as in a low concentrations, a wash performance at least that of the parent polypeptide having SEQ ID NO: 15. Specifications of the model detergents and specific test conditions for the AMSA, can be seen in Examples 3 and 4.

In another embodiment, the variant is selected from the group consisting of; G182*+D183*+N195F+V206F+N260P+L3121+L313I+R320A+T355L+L389I+T400P, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+G50A+T51A+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G476K, H1*+N54S+V56T+M105F+G109A+A174S+G182*+D183*+N195F+V206L+R320A+S323N+K391A+G476K, H1*+T40K+N54S+V56T+G109A+G149H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N16H+V17L+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+Q22N+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+Q32S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+H28N+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N29S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+H28N+N29S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+R176K+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+R176K+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+N175G+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+Y295N+Q299T+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K269S+A274K+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L2351+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L235V+V238A+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Q365S+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+H402R+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320S+H321N+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q125S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L235V+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A265G+K391A+G476K, H1*+T51Q+N54S+V56T+G109A+A174S+G182*+D183*+N195F+Y203F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N175Q+G182*+D183*+N195F+V206L+K391A+G476K, H1*+H28N+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+G50A+T51A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+G7K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+M202V+V206L+K391A+G476K, H1*+N54S+V56T+M105F+G109A+A174S+G182*+D183*+N195F+V206L+M246F+K391A+G476K, H1*+N16H+V17L+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+T51G+N54S+V56T+G109A+A174S+G182*+D183*+N195F+Y203G+V206L+K391A+G476K, H1*+T51G+N54S+V56T+G109A+G110N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q125S+A174S+N175G+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A265G+V291A+F328L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246F+A265G+V291A+F328L+Q365S+K391A+G476K, H1*+N16Y+N54S+V56T+G109A+R116H+A174S+G182*+D183*+N195F+V206L+G337E+D377H+K391A+G476K, H1*+19L+N54S+V56T+G109A+Q125S+V131T+A174*+G182*+D183*+V206L+Y267F+F289H+Q361E+K391A+G476K, H1*+N54S+V56T+G109A+R116H+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+P473R+G476K, H1*+N54S+V56T+G109A+Q169E+Q172G+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+K391A+P473R+G476K, H1*+N54S+V56T+G109A+R116W+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206F+K391A+G476K, H1*+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N260G+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+Y295N+K391A+G476K, H1*+N54S+V56T+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+A186N+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+A288V+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+W48F+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+V291A+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+R320A+S323N+K391A+G476K, H1*+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116H+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N270T+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+N260G+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+A186D+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+A225V+K391A+G476K, H1*+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G255S+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K269Q+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255S+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, G182*+D183*+N195F, H1*+N54S+V56T+G109A+Q169E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+W284H+K391A+G476K, H1*+N54S+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+S304R+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246V+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+N270G+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+Y295F+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174T+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W140Y+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+F328L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G476K, F113Q+R116H+D183*+G182*+N195F, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A288L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N270G+K391A+G476K, Q169E+Q172K+A174*+D183*+G182*+N195F, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K269Q+K391A+G476K, H1*+W48F+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, F113Q+R116Q+D183*+G182*+N195F, N54S+V56T+D183*+G182*+N195F, H1*+N54S+V56T+G109A+A174T+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116W+A174S+G182*+D183*+N195F+V206L+K391A+G476K, Q169E+Q172K+G182*+D183*+N195F, G182*+D183*+A186D, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238A+K391A+G476K, G182*+D183*+A186N, H1*+N54S+V56T+G109A+A174S+V206L+G182*+D183*+K391A+G476K, H1*+N54S+V56T+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206F+K391A+G476K, H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+A186N+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F289L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y295F+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S244Q+K391A+G476K, G182*+D183*+F328L, G182*+D183*+N195F+A288V, G182*+D183*+A186N+N195F, G182*+D183*+E190P+N195F, W140Y+G182*+D183*+N195F, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A225V+K391A+G476K, G182*+D183*+A186D+N195F, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+W48Y+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, G182*+D183*+N195F+V238A, G182*+D183*+Y295N, Q172G+G182*+D183*, F113Q+G182*+D183*+N195F, H1*+N54S+V56T+Q98A+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, G182*+D183*+N195F+Y295F, G182*+D183*+A186E, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206Y+K391A+G476K, G182*+D183*+N195F+W284H, G182*+D183*+A186Q+N195F, K72R+G182*+D183*, D183*+G182*+N195F+V326L, D183*+G182*+A186E+N195F, Q169E+G182*+D183*+N195F, D183*+G182*+N195F+K242Q, G7A+G182*+D183*+N195F, D183*+G182*+N195F+N260G, D183*+G182*+R321A+S323N, D183*+G182*+W284H, H1*+D183*+G182*, D183*+G182*+A186H+N195F, D183*+G182*+F289V, D183*+G182*+K391A, D183*+G182*+S304R, W167F+G182*+D183*+N195F, F113Q+R116H+D183*+G182*, D183*+G182*+N270G, D183*+G182*+S244Q, H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, D183*+G182*+N195F+Y295N, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, D183*+G182*+A186Q, D183*+G182*+N195F+V206F, D183*+G182*+N195F+R320A, G182*+D183*+G184T+N195F, N54S+V56T+D183*+G182*, W48Y+D183*+G182*, A174S+D183*+G182*, G7A+D183*+G182*, N54S+D183*+G182*, D183*+G182*+N195F+M246V, W48Y+D183*+G182*+N195F, D183*+G182*+N195F+S304R,

wherein numbering is according to SEQ ID NO: 13, and wherein the IF is determined by use of Model A detergent.

In one embodiment, the IF is determined by use of Model A detergent at a temperature of 15° C. In a particular embodiment, the variants have an IF of >1 as compared to the parent polypeptide, wherein the IF is determined by use of Model A detergent at a temperature of 15° C. and a concentration of 0.05 mg/L. It can be seen from the Tables in the Examples herein disclosed which such variants may be.

In another embodiment, the variant has an IF of at least 1.0 when compared to the parent polypeptide when tested in Model J detergent, and wherein the variant is selected from the group consisting of: G182*+D183*+N195F, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+R87S+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T40G+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72H+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37H+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37M+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37Y+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37R+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113W+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q118N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116W+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116L+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+G142T+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q125P+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q125K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S381G+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246T+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V213T+K391A+G476K, H1*+A37L+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q118K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q125A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377S+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377G+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377R+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377A+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214G+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S381A+K391A+G476K, H1*+N54S+V56T+G109A+W1671+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W167G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346S+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346P+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214S+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214T+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420Q+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420S+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420K+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420L+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391Y+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391R+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473G+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473T+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+Q395P+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444S+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444D+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444Y+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280W+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280T+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280A+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255N+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T285L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T285Q+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+Q449T+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473K+G476K, H1*+N54S+V56T+K72S+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T40K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346T+K391A+G476K, H1*+G50A+N54S+V56T+G109A++Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484A, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484G, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484P, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484E, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484Q, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484S, H1*K+G50A+N54S+V56T+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+L173V+A174S+G182*+D183*+N195F+A204T+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W140Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+Q98S+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+H324L+K391A+G476K, H1*+N54S+V56T+G109A+G149Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346T+K391A+G476K+G477A, H1*+N54S+V56T+G109AG255A++A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+G50A+N54S+V56T+G109A+F113L+R116L+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+G477Q, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Q345D+K391A+G476K+G477Q, H1*+G50A+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V2641+K391A+G423H+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320V+K391A+G476Y, H1*+T5K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L217V+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L217H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+P211D+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391Q+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444H+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444R+G476K, H1*+G50A+N54S+V56T+G109A+L173V+A174S+G182*+D183*+N195F+A204V+V206L+K391A+G476K, H1*+G50A+N54S+V56T+G109A+F113L+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*°G7K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K, H1*+N54S+V56T+G109A+Q118G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280K+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N270G+K391A+G476K, H1*+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+G346T+K391A+G476K+G477A, H1*+N54S+V56T+G109A+Q118S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G337H+Q385L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y382M+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+K72R+M105F+G109A+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+M105F+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+M105F+G109A+Q118F+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+S323N+D377H+K391A+G476K, H1*+T51E+N54S+V56T+G109A+A174H+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+L173A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Q299V+K391A+G476K, H1*+N54S+V56T+G109A+L173G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T355L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T355F+K391A+G476K, H1*+G50A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204T+V206L+K391A+G476K, H1*+G50A+N54S+V56T+G109A+A174E+G182*+D183*+N195F+A204T+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+R320A+S323N+K391A+G476K, H1*+N54S+V56T+M105I+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246L+K391A+G476K, H1*+T51G+N54S+V56T+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51A+N54S+V56T+G109A+A174G+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174D+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51S+N54S+V56T+G109A+A174P+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51G+N54S+V56T+G109A+A174M+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+W167H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+Q11H+N54S+V56T+G109A+Q118R+T134E+A174S+G184T+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+G109A+Q118R+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+Q172D+A174Q+G182*+D183*+N195F+V206L+K391A+G476K+G477A, H1*+N54S+V56T+K72A+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W167S+G182*+D183*+N195F+A174S+V206L+K391A+G476K, H1*+N54S+V56T+K72H+G109A+W167S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+W167T+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72E+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+W167L+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+W167M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+W167H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K, H1*+N54S+V56T+G109A+Q118R+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184A+T193K+N195F+V206L+K391A+G476K, H1*+N54S+V56T+M105F+G109A+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+R320A+S323N+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291G+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+F328L+D377H+K391A+G476K, H1*+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238A+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238T+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238G+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F328M+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F328L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F328V+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F328I+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291T+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+D377H+K391A+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+G109A+L173H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q125T+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N16S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72N+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72N+G109A+W167L+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72A+G109A+W167Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+G184T+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q118R+A174S+G182*+D183*+G184T+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q118R+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+R320A+S323N+K391A+G476K, H1*+N54S+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377R+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L217T+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377S+K391A+G476K, H1*+N54S+V56T+G109A+R116E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L217Q+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346D+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377A+K391A+G476K, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346P+K391A+G476K, H1*+N54S+V56T+G109A+R116S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377Q+K391A+G476K, H1*+N54S+V56T+G109A+R116I+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174F+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174T+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+T51G+N54S+V56T+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51A+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51S+N54S+V56T+G109A+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51A+N54S+V56T+G109A+Q172S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51E+N54S+V56T+G109A+Q172T+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51A+N54S+V56T+G109A+Q172A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51S+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51G+N54S+V56T+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51A+N54S+V56T+G109A+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51E+N54S+V56T+G109A+Q172L+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51E+N54S+V56T+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51D+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51A+N54S+V56T+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q172S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72H+G109A+Q172T+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q172R+A174S+K179E+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q172M+A174S+G182*+D183*+N195F+V206L+Y295N+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q172T+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72M+G109A+Q172S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72S+G109A+A174H+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+A174D+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174D+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+A174K+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174G+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174Q+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+A174G+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72S+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174H+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391S+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444A+G476K, H1*+N54S+V56T+G109A+F113L+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+G50A+N54S+V56T+G109A+A174E+G182*+D183*+N195F+A204T+V206L+S323N+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q118R+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+M105F+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q118R+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A225F+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246A+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y3821+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S376H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214E+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214W+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204T+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204S+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+T165S+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T334S+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A263G+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M286F+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y2671+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L235V+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L235A+D377H+K391A+G476K, H1*+N54S+V56T+M105L+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+M105I+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+M105V+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M2461+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L2501+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250V+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391Y+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391M+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391E+G476K, H1*+N54S+V56T+G109A+T165G+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54R+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54L+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174V+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246F+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246V+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246S+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174R+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174L+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206S+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391D+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391R+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391H+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391W+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K3911+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250T+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250A+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250F+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250M+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391Q+G476K, H1*+N54S+V56T+G109A+Q172H+L173A+A174P+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172E+L173A+A174R+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+W167R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+G184T+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+G255A+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S376T+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S376V+K391A+G476K, H1*+N54S+V56T+G109A+Q172E+L1731+A174N+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172D+L173A+A174T+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267M+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267H+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238A+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238T+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238G +K391A+G476K, H1*+N54S+V56T+M105V+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V264T+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V264I+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L235V+K391A+G476K, H1*+N54S+V56T+G109A+W167H+Q172N+L173A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W167F+Q172E+L173P+A174K+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172N+L173T+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172K+L173V+A174T+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172E+L173F+A174R+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172E+L173T+A174R+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172N+L173V+A174R+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+L173V+A174R+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172E+L173M+A174H+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172E+L173F+A174P+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W167Y+Q172E+L173V+A174H+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+L173A+A174T+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+F328L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246S+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250V+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250F+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250M+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204S+V206L+K391A+G476K, H1*+N54S+V56T+G109A+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T334S+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A263G+M286F+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A263G+M286L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y2671+K391A+G476K, H1*+T51A+N54S+V56T+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391L+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109S+F113Q+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+A204G+V206L+G255A+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+W167F+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+G184T+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+P473G+G476K, H1*+N54S+V56T+G109A+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+M105F+G109A+A174S+G182*+D183*+N195F+V206L+R320A+S323N+K391A+C474V+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+G255A+K391A+C474V+G476K, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+C474V+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A265G+K391A+T444Q+G476K+G477A, H1*+A37V+N54S+V56T+G109A+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G346T+G477A+G476K, H1*+N54S+V56T+G109A+R116Q+Q172D+A174S+G182*+D183*+N195F+V206L+G346T+K391A+T444Q+G477A+G476K, H1*+N54S+V56T+G109A+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+G346T+K391A+T444Q+G477A+G476K, H1*+N54S+V56T+G109S+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109S+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109S+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+V2641+K391A+C474V+G476K, H1*+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280Q+H321Y+K391A+C474V+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+C474V+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109A+F113Q+R116Q+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+R116H+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+T165G+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116Q+T165G+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+F2891+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116Q+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116H+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+T165G+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+Q395P+G476K, H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+A265G+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+K72R+G109A+T134E+T165G+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+A265G+K391A+P473R+G476K, H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51V+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280Q+H321Y+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109A+R116Q+W167F+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+G133Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+Q395P+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+K72R+G109A+R116H+W167F+Q172R+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+G184T+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109S+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109S+R116Q+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109S+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+G109S+F113Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+R116H+T134E+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+K72R+G109A+R116H+T134E+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+G255A+K391A+Q395P+T444Q+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+T134E+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+G255A+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+T134E+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+G255A+K391A+Q395P+A445Q+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116H+T134E+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+G255A+K391A+Q395P+G476K, H1*+N54S+V56T+K72R+G109A+T134E+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+G255A+A265G+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+K72R+G109A+G133Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172N+L173V+A174S+G182*+D183*+G184T+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+A37H+N54S+V56T+G109A+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37H+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+T459N+G476K, H1*+N54S+V56T+G109A+F113Q+Q172R+L173V+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+A60V+G109A+R116H+Q172R+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37H+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37H+N54S+V56T+A60V+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37H+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116A+W167F+Q172R+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+R116A+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+R116A+T165G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109A+G182*+D183*+N195F+K391A+G476K, H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L, N54S+V56T+G109A+G182*+D183*+N195F, N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+G476K, H1*+N54S+V56T+A174S+G182*+D183*+N195F+V206L+G273R+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L, H1*+W48G+G109A+G182*+D183*+N195F+V206L+K391A+G476K, N54S+V56T+G109A+A174S+G182*+D183*+N195F+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+K391A, H1*+N54S+V56T+G109A+F113Q+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+R116Q+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+G109A+R116A+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+M246F+K391A+G476K, H1*+V56A+G109A+A174S+G182*+D183*+N195F+V206L+G476K, H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+K391A, H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+G476K, G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, N54S+V56T+G109A+G182*+D183*+N195F+V206L+K391T+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116H+A174S+G182*+D183*+G184T+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109S+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+F113Q+R116Q+Q172N+A174S+G182*+D183*+N195F+V206L+A265G+K391A+P473R+G476K, H1*+N54S+V56T+G109A+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+R116A+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109A+R116H+Q172R+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K, H1*+A37V+N54S+V56T+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37H+N54S+V56T+G109A+R116H+T165G+A174S+G182*+D183*+N195F+V206L+M246F+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+K391A+G476K, H1*+G109A+G182*+D183*+N195F+V206L+K391A+G476K, G109A+G182*+D183*+N195F+V206L+G476K, H1*+G109A+G182*+D183*+N195F+K391A, H1*+N54S+V56T+G109A+G133S+A174S+G182*+D183*+N195F+K391A+G476H, H1*+N54S+V56T+G109A+R116A+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+G109A+R116H+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+P45A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+P124A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+P364A+K391A+C474V+G476K, H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+G50A+T51A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V264F+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y382L+K391A+G476K, H1*+G7K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255A+Q256A+K391A+G476K, H1*+N54S+V56T+G109A+W167Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W167H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+T165V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174Q+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N16H+V17L+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y382F+K391A+G476K, H1*+Q32S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Q385L+K391A+G476K, H1*+N54S+V56T+R87S+G109A+R171H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, N54S+G182*+D183*+N195F, G109A+G182*+D183*+N195F, A174S+G182*+D183*+N195F, G182*+D183*+N195F+K391A, G182*+D183*+N195F+G476K, H1*+N54S+V56T+G109A+R116H+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+P473R+G476K, H1*+N54S+V56T+G109A+Q169E+Q172G+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+K391A+P473R+G476K, H1*+N54S+V56T+G109A+R116W+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206F+K391A+G476K, H1*+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N260G+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+Y295N+K391A+G476K, H1*+N54S+V56T+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+A186N+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+A288V+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+W48F+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+V291A+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+R320A+S323N+K391A+G476K, H1*+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116H+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N270T+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+N260G+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+A186D+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+A225V+K391A+G476K, H1*+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G255S+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K269Q+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255S+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, G182*+D183*+N195F, H1*+N54S+V56T+G109A+Q169E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+W284H+K391A+G476K, H1*+N54S+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+S304R+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246V+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+N270G+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+Y295F+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174T+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W140Y+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+F328L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G476K, F113Q+R116H+D183*+G182*+N195F, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A288L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N270G+K391A+G476K, Q169E+Q172K+A174*+D183*+G182*+N195F, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K269Q+K391A+G476K, H1*+W48F+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, F113Q+R116Q+D183*+G182*+N195F, N54S+V56T+D183*+G182*+N195F, H1*+N54S+V56T+G109A+A174T+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116W+A174S+G182*+D183*+N195F+V206L+K391A+G476K, Q169E+Q172K+G182*+D183*+N195F, G182*+D183*+A186D, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238A+K391A+G476K, G182*+D183*+A186N, H1*+N54S+V56T+G109A+A174S+V206L+G182*+D183*+K391A+G476K, H1*+N54S+V56T+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206F+K391A+G476K, H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+A186N+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F289L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y295F+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S244Q+K391A+G476K, G182*+D183*+F328L, G182*+D183*+N195F+A288V, G182*+D183*+A186N+N195F, G182*+D183*+E190P+N195F, W140Y+G182*+D183*+N195F, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A225V+K391A+G476K, G182*+D183*+A186D+N195F, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+W48Y+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, G182*+D183*+N195F+V238A, G182*+D183*+Y295N, Q172G+G182*+D183*, F113Q+G182*+D183*+N195F, H1*+N54S+V56T+Q98A+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, G182*+D183*+N195F+Y295F, G182*+D183*+A186E, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206Y+K391A+G476K, G182*+D183*+N195F+W284H, G182*+D183*+A186Q+N195F, K72R+G182*+D183*, D183*+G182*+N195F+V326L, D183*+G182*+A186E+N195F, Q169E+G182*+D183*+N195F, D183*+G182*+N195F+K242Q, G7A+G182*+D183*+N195F, D183*+G182*+N195F+N260G, D183*+G182*+R321A+S323N, D183*+G182*+W284H, H1*+D183*+G182*, D183*+G182*+A186H+N195F, D183*+G182*+F289V, D183*+G182*+K391A, D183*+G182*+S304R, W167F+G182*+D183*+N195F, F113Q+R116H+D183*+G182*, D183*+G182*+N270G, D183*+G182*+S244Q, H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, D183*+G182*+N195F+Y295N, W48F+D183*+G182*, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, D183*+G182*+A186Q, W140Y+D183*+G182*, D183*+G182*+K242Q, D183*+G182*+V206L, D183*+G182*+N195F+V206F, Q169E+D183*+G182*, D183*+G182*+N195F+R320A, G182*+D183*+G184T+N195F, D183*+G182*+G184T, D183*+G182*+P473R, W48Y+D183*+G182*, D183*+G182*+R323A, G7A+D183*+G182*, H1*+N54S+V56T+G109A+Q169E+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, A174S+D183*+G182*, D183*+G182*+N195F+S323N, D183*+G182*+N195F+M246V, W48Y+D183*+G182*+N195F, V56T+D183*+G182*, wherein numbering is according to SEQ ID NO: 13, G182*+D183*+N195F+A428T, G182*+D183*+N195F, H1*+G182*+D183*+N195F, N54S+G182*+D183*+N195F, V56T+G182*+D183*+N195F, G182*+D183*+N195F+I405L, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T+D476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+A174S+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476G, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476K+G477S, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476K, H1*+N54S+V56T+K72R+G109A+W167F+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280Q+H321Y+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S304N+I405L+A421H+A422P+A428T+D476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476K+G477A, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476G+G477T, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+G477Q, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+G477A, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476K+G477Q, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476N, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476Y, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V2061+1405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+A204G+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+A204G+V206L+G255A+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+R116H+T165G+Q172G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+Q172N+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+G196R+V206L+G255A+I405L+A421H+A422P+A428T+N475S, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+T40D+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+N125D+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+A60V+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+R116V+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+R116Q+G133Q+T165G+Q172G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+R116Q+Q172G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T+G448D, H1*+N54S+V56T+G109A+F113Q+R116H+Q172N+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+T134E+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+G255A+A265G+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+R116Q+W167F+Q172R+L173V+A174S+G182*+D183*+N195F+V206L+A265G+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+F113Q+T134E+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+G255A+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+G184T+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+G255A+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+R116Q+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+N195F+A204G+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+A265G+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+A37H+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+A174S+M105F+G182*+D183*+N195F+V206L+L2281+R320A+S323N+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+F113Q+T134E+A174S+G182*+D183*+N195F+V206L+G255A+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+R116Q+V120L+A174S+G182*+D183*+G184T+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+R116Q+Q172N+A174S+G182*+D183*+N195F+V206L+A265G+I405L+A421H+A422P+A428T, H1*+A37H+N54S+V56T+A60V+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, wherein numbering is according to SEQ ID NO: 14.

In one embodiment, the IF is determined by use of Model J detergent at a temperature of 15° C. In a particular embodiment, the variants have an IF of >1 as compared to the parent polypeptide, wherein the IF is determined by use of Model J detergent at a temperature of 15° C. and a concentration of 0.05mg/L. It can be seen from the Tables in the Examples herein disclosed which such variants may be.

In another embodiment, the variant has an IF of at least >1.5 when compared to said parent polypeptide, and wherein said variant is selected from H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+R87S+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T40G+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72H+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37H+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37M+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37Y+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37R+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113W+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q118N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116W+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116L+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+G142T+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q125P+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q125K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S381G+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246T+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V213T+K391A+G476K, H1*+A37L+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q118K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q125A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377S+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377G+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377R+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377A+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214G+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S381A+K391A+G476K, H1*+N54S+V56T+G109A+W1671+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W167G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346S+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346P+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214S+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214T+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420Q+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420S+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420K+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420L+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391Y+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391R+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473G+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473T+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+Q395P+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444S+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444D+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444Y+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280W+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280T+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280A+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255N+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T285L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T285Q+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+Q449T+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473K+G476K, H1*+N54S+V56T+K72S+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T40K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346T+K391A+G476K, H1*+G50A+N54S+V56T+G109A++Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484A, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484G, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484P, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484E, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484Q, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484S, H1*K+G50A+N54S+V56T+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+L173V+A174S+G182*+D183*+N195F+A204T+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W140Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+Q98S+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+H324L+K391A+G476K, H1*+N54S+V56T+G109A+G149Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346T+K391A+G476K+G477A, H1*+N54S+V56T+G109AG255A++A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+G50A+N54S+V56T+G109A+F113L+R116L+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+G477Q, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Q345D+K391A+G476K+G477Q, H1*+G50A+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V264I+K391A+G423H+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320V+K391A+G476Y, H1*+T5K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L217V+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L217H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+P211D+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391Q+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444H+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444R+G476K, H1*+G50A+N54S+V56T+G109A+L173V+A174S+G182*+D183*+N195F+A204V+V206L+K391A+G476K, H1*+G50A+N54S+V56T+G109A+F113L+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+G7K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K, H1*+N54S+V56T+G109A+Q118G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280K+K391A+G476K, H1*+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+G346T+K391A+G476K+G477A, H1*+N54S+V56T+G109A+Q118S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G337H+Q385L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y382M+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+K72R+M105F+G109A+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+M105F+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+M105F+G109A+Q118F+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+S323N+D377H+K391A+G476K, H1*+T51E+N54S+V56T+G109A+A174H+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+L173A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Q299V+K391A+G476K, H1*+N54S+V56T+G109A+L173G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T355L+K391A+G476K, H1*+G50A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204T+V206L+K391A+G476K, H1*+G50A+N54S+V56T+G109A+A174E+G182*+D183*+N195F+A204T+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+R320A+S323N+K391A+G476K, H1*+N54S+V56T+M105I+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246L+K391A+G476K, H1*+T51G+N54S+V56T+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174D+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51S+N54S+V56T+G109A+A174P+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72A+G109A+W167S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+W167H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+Q11H+N54S+V56T+G109A+Q118R+T134E+A174S+G184T+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+G109A+Q118R+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+Q172D+A174Q+G182*+D183*+N195F+V206L+K391A+G476K+G477A, H1*+N54S+V56T+K72R+G109A+W167L+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+W167M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K, H1*+N54S+V56T+M105F+G109A+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+R320A+S323N+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291G+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+F328L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238A+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238T+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238G+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F328M+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F328L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F328V+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F3281+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291T+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+D377H+K391A+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+G109A+L173H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N16S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72N+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72N+G109A+W167L+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72A+G109A+W167Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+G184T+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q118R+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+R320A+S323N+K391A+G476K, H1*+N54S+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L217T+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377S+K391A+G476K, H1*+N54S+V56T+G109A+R116E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L217Q+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377A+K391A+G476K, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346P+K391A+G476K, H1*+N54S+V56T+G109A+R116S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377Q+K391A+G476K, H1*+N54S+V56T+G109A+R1161+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174F+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174T+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+T51A+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51S+N54S+V56T+G109A+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51A+N54S+V56T+G109A+Q172S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51E+N54S+V56T+G109A+Q172T+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51A+N54S+V56T+G109A+Q172A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51S+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51A+N54S+V56T+G109A+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51E+N54S+V56T+G109A+Q172L+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51E+N54S+V56T+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51D+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51A+N54S+V56T+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q172S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72H+G109A+Q172T+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q172R+A174S+K179E+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q172M+A174S+G182*+D183*+N195F+V206L+Y295N+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q172T+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72M+G109A+Q172S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72S+G109A+A174H+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+A174D+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174D+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+A174K+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174G+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174Q+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+A174G+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72S+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174H+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391S+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444A+G476K, H1*+N54S+V56T+G109A+F113L+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+G50A+N54S+V56T+G109A+A174E+G182*+D183*+N195F+A204T+V206L+S323N+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q118R+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+M105F+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q118R+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A225F+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246A+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y3821+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S376H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214E+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214W+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204T+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204S+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+T165S+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T334S+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A263G+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M286F+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y2671+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L235V+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L235A+D377H+K391A+G476K, H1*+N54S+V56T+M105L+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+M105I+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+M105V+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M2461+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L2501+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250V+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391Y+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391M+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391E+G476K, H1*+N54S+V56T+G109A+T165G+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54L+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174V+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246F+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246V+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246S+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174R+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174L+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206S+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391D+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391R+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391H+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391W+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K3911+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250T+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250A+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250F+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250M+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391Q-FG476K, H1*+N54S+V56T+G109A+Q172H+L173A+A174P+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+W167R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+G184T+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+G255A+D377H+K391A+G476K, H1*+N54S+V56T+G109A+Q118R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S376T+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S376V+K391A+G476K, H1*+N54S+V56T+G109A+Q172E+L1731+A174N+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172D+L173A+A174T+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267M+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267H+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238A+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238T+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238G +K391A+G476K, H1*+N54S+V56T+M105V+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V264T+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V2641+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L235V+K391A+G476K, H1*+N54S+V56T+G109A+W167H+Q172N+L173A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W167F+Q172E+L173P+A174K+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172N+L173T+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172K+L173V+A174T+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172E+L173F+A174R+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172E+L173T+A174R+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172N+L173V+A174R+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+L173V+A174R+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172E+L173M+A174H+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172E+L173F+A174P+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W167Y+Q172E+L173V+A174H+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+L173A+A174T+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+F328L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246S+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250V+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250F -FK391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250M+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204S+V206L+K391A+G476K, H1*+N54S+V56T+G109A+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T334S+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A263G+M286L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267L+K391A+G476K, H1*+T51A+N54S+V56T+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391L+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109S+F113Q+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+A204G+V206L+G255A+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+W167F+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+G184T+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+P473G+G476K, H1*+N54S+V56T+G109A+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+M105F+G109A+A174S+G182*+D183*+N195F+V206L+R320A+S323N+K391A+C474V+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+G255A+K391A+C474V+G476K, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+C474V+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A265G+K391A+T444Q+G476K+G477A, H1*+A37V+N54S+V56T+G109A+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G346T+G477A+G476K, H1*+N54S+V56T+G109A+R116Q+Q172D+A174S+G182*+D183*+N195F+V206L+G346T+K391A+T444Q+G477A+G476K, H1*+N54S+V56T+G109A+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+G346T+K391A+T444Q+G477A+G476K, H1*+N54S+V56T+G109S+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109S+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109S+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+V264I+K391A+C474V+G476K, H1*+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280Q+H321Y+K391A+C474V+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+C474V+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109A+F113Q+R116Q+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+R116H+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+T165G+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116Q+T165G+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+F289I+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116Q+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116H+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+T165G+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+Q395P+G476K, H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+A265G+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+K72R+G109A+T134E+T165G+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+A265G+K391A+P473R+G476K, H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51V+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280Q+H321Y+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109A+R116Q+W167F+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+G133Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+Q395P+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+K72R+G109A+R116H+W167F+Q172R+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+G184T+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109S+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109S+R116Q+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109S+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+G109S+F113Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+R116H+T134E+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+K72R+G109A+R116H+T134E+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+G255A+K391A+Q395P+T444Q+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+T134E+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+G255A+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+T134E+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+G255A+K391A+Q395P+A445Q+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116H+T134E+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+G255A+K391A+Q395P+G476K, H1*+N54S+V56T+K72R+G109A+T134E+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+G255A+A265G+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+K72R+G109A+G133Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172N+L173V+A174S+G182*+D183*+G184T+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+A37H+N54S+V56T+G109A+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37H+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+T459 N+G476K, H1*+N54S+V56T+G109A+F113Q+Q172R+L173V+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+A60V+G109A+R116H+Q172R+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37H+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37H+N54S+V56T+A60V+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37H+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116A+W167F+Q172R+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+R116A+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+R116A+T165G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109A+G182*+D183*+N195F+K391A+G476K, H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L, N54S+V56T+G109A+G182*+D183*+N195F, N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+G476K, H1*+N54S+V56T+A174S+G182*+D183*+N195F+V206L+G273R+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L, H1*+W48G+G109A+G182*+D183*+N195F+V206L+K391A+G476K, N54S+V56T+G109A+A174S+G182*+D183*+N195F+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+R116Q+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+G109A+R116A+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+M246F+K391A+G476K, H1*+V56A+G109A+A174S+G182*+D183*+N195F+V206L+G476K, H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+K391A, H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+G476K, G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, N54S+V56T+G109A+G182*+D183*+N195F+V206L+K391T+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116H+A174S+G182*+D183*+G184T+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109S+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+F113Q+R116Q+Q172N+A174S+G182*+D183*+N195F+V206L+A265G+K391A+P473R+G476K, H1*+N54S+V56T+G109A+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+R116A+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109A+R116H+Q172R+A174S+G182*+D183*+N195F+A204G-FV206L+K391A+P473R+G476K, H1*+A37V+N54S+V56T+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37H+N54S+V56T+G109A+R116H+T165G+A174S+G182*+D183*+N195F+V206L+M246F+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+K391A+G476K, H1*+G109A+G182*+D183*+N195F+V206L+K391A+G476K, G109A+G182*+D183*+N195F+V206L+G476K, H1*+G109A+G182*+D183*+N195F+K391A, H1*+N54S+V56T+G109A+G133S+A174S+G182*+D183*+N195F+K391A+G476H, H1*+N54S+V56T+G109A+R116A+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+G109A+R116H+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+P45A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+P124A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+P364A+K391A+C474V+G476K, H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+G50A+T51A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V264F+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y382L+K391A+G476K, H1*+G7K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255A+Q256A+K391A+G476K, H1*+N54S+V56T+G109A+W167Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W167H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+T165V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174Q+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N16H+V17L+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y382F+K391A+G476K, H1*+Q32S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Q385L+K391A+G476K, H1*+N54S+V56T+R87S+G109A+R171H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, G109A+G182*+D183*+N195F, G182*+D183*+N195F+K391A, H1*+N54S+V56T+G109A+R116H+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+P473R+G476K, H1*+N54S+V56T+G109A+Q169E+Q172G+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+K391A+P473R+G476K, H1*+N54S+V56T+G109A+R116W+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206F+K391A+G476K, H1*+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N260G+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+Y295N+K391A+G476K, H1*+N54S+V56T+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+A186N+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+A288V+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+W48F+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+V291A+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+R320A+S323N+K391A+G476K, H1*+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116H+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N270T+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+N260G+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+A186D+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+A225V+K391A+G476K, H1*+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G255S+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K269Q+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255S+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, G182*+D183*+N195F, H1*+N54S+V56T+G109A+Q169E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+W284H+K391A+G476K, H1*+N54S+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+S304R+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246V+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+N270G+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+Y295F+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174T+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W140Y+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+F328L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G476K, F113Q+R116H+D183*+G182*+N195F, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A288L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N270G+K391A+G476K, Q169E+Q172K+A174*+D183*+G182*+N195F, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K269Q+K391A+G476K, H1*+W48F+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, F113Q+R116Q+D183*+G182*+N195F, N54S+V56T+D183*+G182*+N195F, H1*+N54S+V56T+G109A+A174T+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116W+A174S+G182*+D183*+N195F+V206L+K391A+G476K, Q169E+Q172K+G182*+D183*+N195F, G182*+D183*+A186D, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238A+K391A+G476K, G182*+D183*+A186N, H1*+N54S+V56T+G109A+A174S+V206L+G182*+D183*+K391A+G476K, H1*+N54S+V56T+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206F+K391A+G476K, H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+A186N+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F289L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y295F+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+K391A+G476K, G182*+D183*+A186N+N195F, G182*+D183*+A186D+N195F, G182*+D183*+N195F+W284H, D183*+G182*+N195F+V326L, wherein numbering is according to SEQ ID NO: 13, and H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476G+G477T, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+G477Q, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+G477A, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476N, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476Y, H1*+N54S+V56T+G109A+A1745+G182*+D183*+N195F+V2061+1405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+T40D+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+N125D+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+A60V+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+R116H+Q172N+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+T134E+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+G255A+A265G+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+R116Q+W167F+Q172R+L173V+A174S+G182*+D183*+N195F+V206L+A265G+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+F113Q+T134E+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+G255A+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+G184T+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+G255A+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+R116Q+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+F113Q+F116H+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+N195F+A204G+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+A265G+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+A37H+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+F113Q+T134E+A174S+G182*+D183*+N195F+V206L+G255A+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+R116Q+V120L+A174S+G182*+D183*+G184T+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+R116Q+Q172N+A174S+G182*+D183*+N195F+V206L+A265G+I405L+A421H+A422P+A428T, H1*+A37H+N54S+V56T+A60V+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, wherein numbering is according to SEQ ID NO: 14, and wherein the IF is determined by use of Model A detergent.

In one embodiment, the IF is determined by use of Model A detergent at a temperature of 15° C. In a particular embodiment, the variants have an IF of >1.5 as compared to the parent golvaeatide, wherein the IF is determined by use of Model A detergent at a temperature of 15° C. and a concentration of 0.05 mg/L. It can be seen from the Tables in the Examples herein disclosed which such variants may be.

In another embodiment, the variant has an IF of at least 1.5 when compared to the parent polypeptide and when determined by use of Model J detergent, wherein the variant is selected from the group consisting of: G182*+D183*+N195F, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+R87S+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T40G+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72H+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37H+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37M+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37Y+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37R+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113W+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q118N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116L+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+G142T+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q125P+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q125K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S381G+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246T+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V213T+K391A+G476K, H1*+A37L+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q118K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q125A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377S+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377G+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377R+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377A+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214G+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S381A+K391A+G476K, H1*+N54S+V56T+G109A+W1671+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W167G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346S+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346P+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214S+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214T+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420Q+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420S+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420K+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420L+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391Y+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391R+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473G+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473T+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+Q395P+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444S+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444D+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444Y+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280W+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280T+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280A+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255N+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T285L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T285Q+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+Q449T+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473K+G476K, H1*+N54S+V56T+K72S+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T40K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346T+K391A+G476K, H1*+G50A+N54S+V56T+G109A++Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484A, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484G, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484P, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484E, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484Q, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484S, H1*K+G50A+N54S+V56T+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+L173V+A174S+G182*+D183*+N195F+A204T+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W140Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+Q98S+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+H324L+K391A+G476K, H1*+N54S+V56T+G109A+G149Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346T+K391A+G476K+G477A, H1*+N54S+V56T+G109AG255A++A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+G50A+N54S+V56T+G109A+F113L+R116L+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+G477Q, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Q345D+K391A+G476K+G477Q, H1*+G50A+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V2641+K391A+G423H+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320V+K391A+G476Y, H1*+T5K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L217V+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L217H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+P211D+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391Q+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444H+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444R+G476K, H1*+G50A+N54S+V56T+G109A+L173V+A174S+G182*+D183*+N195F+A204V+V206L+K391A+G476K, H1*+G50A+N54S+V56T+G109A+F113L+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+G7K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K, H1*+N54S+V56T+G109A+Q118G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280K+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N270G+K391A+G476K, H1*+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+G346T+K391A+G476K+G477A, H1*+N54S+V56T+G109A+Q118S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G337H+Q385L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y382M+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+K72R+M105F+G109A+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+M105F+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+M105F+G109A+Q118F+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+S323N+D377H+K391A+G476K, H1*+T51E+N54S+V56T+G109A+A174H+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+L173A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Q299V+K391A+G476K, H1*+N54S+V56T+G109A+L173G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T355L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T355F+K391A+G476K, H1*+G50A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204T+V206L+K391A+G476K, H1*-FG50A+N54S+V56T+G109A+A174E+G182*+D183*+N195F+A204T+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+R320A+S323N+K391A+G476K, H1*+N54S+V56T+M105I+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246L+K391A+G476K, H1*+T51G+N54S+V56T+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51A+N54S+V56T+G109A+A174G+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174D+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51G+N54S+V56T+G109A+A174M+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+W167H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+Q11H+N54S+V56T+G109A+Q118R+T134E+A174S+G184T+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+G109A+Q118R+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+Q172D+A174Q+G182*+D183*+N195F+V206L+K391A+G476K+G477A, H1*+N54S+V56T+K72A+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W167S+G182*+D183*+N195F+A174S+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+W167L+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+W167M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+W167H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K, H1*+N54S+V56T+G109A+Q118R+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K, H1*+N54S+V56T+M105F+G109A+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+R320A+S323N+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291G+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+F328L+D377H+K391A+G476K, H1*+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238A+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238T+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238G+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F328M+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F328L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F328V+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F3281+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291T+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+D377H+K391A+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+G109A+L173H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q125T+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N16S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72N+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72N+G109A+W167L+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72A+G109A+W167Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+G184T+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q118R+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+R320A+S323N+K391A+G476K, H1*+N54S+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377R+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L217T+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377S+K391A+G476K, H1*+N54S+V56T+G109A+R116E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L217Q+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346D+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377A+K391A+G476K, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346P+K391A+G476K, H1*+N54S+V56T+G109A+R116S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377Q+K391A+G476K, H1*+N54S+V56T+G109A+R1161+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174F+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174T+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+T51G+N54S+V56T+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51A+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51S+N54S+V56T+G109A+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51A+N54S+V56T+G109A+Q172S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51E+N54S+V56T+G109A+Q172T+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51A+N54S+V56T+G109A+Q172A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51S+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51G+N54S+V56T+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51A+N54S+V56T+G109A+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51E+N54S+V56T+G109A+Q172L+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51E+N54S+V56T+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51D+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51A+N54S+V56T+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q172S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72H+G109A+Q172T+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q172M+A174S+G182*+D183*+N195F+V206L+Y295N+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q172T+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72M+G109A+Q172S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72S+G109A+A174H+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+A174D+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174D+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+A174K+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174G+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174Q+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+A174G+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72S+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174H+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391S+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444A+G476K, H1*+N54S+V56T+G109A+F113L+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+G50A+N54S+V56T+G109A+A174E+G182*+D183*+N195F+A204T+V206L+S323N+K391A+G476K, H1*+N54S+V56T+M105F+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A225F+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246A+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y3821+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S376H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214E+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214W+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204T+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204S+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+T165S+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T334S+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A263G+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M286F+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y2671+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L235V+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L235A+D377H+K391A+G476K, H1*+N54S+V56T+M105L+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+M105I+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+M105V+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M2461+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L2501+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250V+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391Y+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391M+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391E+G476K, H1*+N54S+V56T+G109A+T165G+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174V+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246F+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246V+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246S+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174R+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174L+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206S+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391D+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391R+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391H+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391W+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391I+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250T+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250A+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250F+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250M+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391Q+G476K, H1*+N54S+V56T+G109A+Q172H+L173A+A174P+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172E+L173A+A174R+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+W167R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+G184T+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+G255A+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S376T+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S376V+K391A+G476K, H1*+N54S+V56T+G109A+Q172E+L173I+A174N+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172D+L173A+A174T+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267M+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267H+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238A+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238T+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238G +K391A+G476K, H1*+N54S+V56T+M105V+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V264T+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V2641+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L235V+K391A+G476K, H1*+N54S+V56T+G109A+W167H+Q172N+L173A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W167F+Q172E+L173P+A174K+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172N+L173T+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172K+L173V+A174T+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172E+L173F+A174R+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172E+L173T+A174R+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172N+L173V+A174R+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+L173V+A174R+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172E+L173M+A174H+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172E+L173F+A174P+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W167Y+Q172E+L173V+A174H+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+L173A+A174T+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+F328L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246S+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250V+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250F+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250M+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204S+V206L+K391A+G476K, H1*+N54S+V56T+G109A+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T334S+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A263G+M286L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y2671+K391A+G476K, H1*+T51A+N54S+V56T+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391L+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109S+F113Q+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+A204G+V206L+G255A+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+W167F+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+G184T+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+P473G+G476K, H1*+N54S+V56T+G109A+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+M105F+G109A+A174S+G182*+D183*+N195F+V206L+R320A+S323N+K391A+C474V+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+G255A+K391A+C474V+G476K, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+C474V+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A265G+K391A+T444Q+G476K+G477A, H1*+A37V+N54S+V56T+G109A+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G346T+G477A+G476K, H1*+N54S+V56T+G109A+R116Q+Q172D+A174S+G182*+D183*+N195F+V206L+G346T+K391A+T444Q+G477A+G476K, H1*+N54S+V56T+G109A+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+G346T+K391A+T444Q+G477A+G476K, H1*+N54S+V56T+G109S+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109S+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109S+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+V2641+K391A+C474V+G476K, H1*+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280Q+H321Y+K391A+C474V+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+C474V+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109A+F113Q+R116Q+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+R116H+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+T165G+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116Q+T165G+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+F2891+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116Q+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116H+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+T165G+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+Q395P+G476K, H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+A265G+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+K72R+G109A+T134E+T165G+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+A265G+K391A+P473R+G476K, H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51V+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280Q+H321Y+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109A+R116Q+W167F+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+G133Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+Q395P+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+K72R+G109A+R116H+W167F+Q172R+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+G184T+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109S+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109S+R116Q+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109S+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+G109S+F113Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+R116H+T134E+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+K72R+G109A+R116H+T134E+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+G255A+K391A+Q395P+T444Q+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+T134E+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+G255A+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+T134E+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+G255A+K391A+Q395P+A445Q+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116H+T134E+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+G255A+K391A+Q395P+G476K, H1*+N54S+V56T+K72R+G109A+T134E+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+G255A+A265G+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+K72R+G109A+G133Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172N+L173V+A174S+G182*+D183*+G184T+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+A37H+N54S+V56T+G109A+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37H+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+T459N+G476K, H1*+N54S+V56T+G109A+F113Q+Q172R+L173V+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+A60V+G109A+R116H+Q172R+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37H+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37H+N54S+V56T+A60V+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37H+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116A+W167F+Q172R+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+R116A+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+R116A+T165G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109A+G182*+D183*+N195F+K391A+G476K, H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L, N54S+V56T+G109A+G182*+D183*+N195F, N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+G476K, H1*+N54S+V56T+A174S+G182*+D183*+N195F+V206L+G273R+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L, H1*+W48G+G109A+G182*+D183*+N195F+V206L+K391A+G476K, N54S+V56T+G109A+A174S+G182*+D183*+N195F+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+K391A, H1*+N54S+V56T+G109A+F113Q+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+R116Q+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+G109A+R116A+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+M246F+K391A+G476K, H1*+V56A+G109A+A174S+G182*+D183*+N195F+V206L+G476K, H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+K391A, H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+G476K, G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, N54S+V56T+G109A+G182*+D183*+N195F+V206L+K391T+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116H+A174S+G182*+D183*+G184T+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109S+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+F113Q+R116Q+Q172N+A174S+G182*+D183*+N195F+V206L+A265G+K391A+P473R+G476K, H1*+N54S+V56T+G109A+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+R116A+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109A+R116H+Q172R+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K, H1*+A37V+N54S+V56T+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37H+N54S+V56T+G109A+R116H+T165G+A174S+G182*+D183*+N195F+V206L+M246F+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+K391A+G476K, H1*+G109A+G182*+D183*+N195F+V206L+K391A+G476K, G109A+G182*+D183*+N195F+V206L+G476K, H1*+G109A+G182*+D183*+N195F+K391A, H1*+N54S+V56T+G109A+G133S+A174S+G182*+D183*+N195F+K391A+G476H, H1*+N54S+V56T+G109A+R116A+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+G109A+R116H+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+P45A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+P124A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+P364A+K391A+C474V+G476K, H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+G50A+T51A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V264F+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y382L+K391A+G476K, H1*+G7K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255A+Q256A+K391A+G476K, H1*+N54S+V56T+G109A+W167Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W167H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+T165V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174Q+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N16H+V17L+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y382F+K391A+G476K, H1*+Q32S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Q385L+K391A+G476K, H1*+N54S+V56T+R87S+G109A+R171H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, N54S+G182*+D183*+N195F, G109A+G182*+D183*+N195F, G182*+D183*+N195F+G476K, H1*+N54S+V56T+G109A+R116H+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+P473R+G476K, H1*+N54S+V56T+G109A+Q169E+Q172G+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+K391A+P473R+G476K, H1*+N54S+V56T+G109A+R116W+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206F+K391A+G476K, H1*+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N260G+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+Y295N+K391A+G476K, H1*+N54S+V56T+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+A186N+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+A288V+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+W48F+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+V291A+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+R320A+S323N+K391A+G476K, H1*+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116H+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N270T+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+N260G+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+A186D+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+A225V+K391A+G476K, H1*+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G255S+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K269Q+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255S+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, G182*+D183*+N195F, H1*+N54S+V56T+G109A+Q169E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+W284H+K391A+G476K, H1*+N54S+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+S304R+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246V+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+N270G+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+Y295F+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174T+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W140Y+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+F328L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G476K, F113Q+R116H+D183*+G182*+N195F, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A288L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N270G+K391A+G476K, Q169E+Q172K+A174*+D183*+G182*+N195F, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K269Q+K391A+G476K, H1*+W48F+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, F113Q+R116Q+D183*+G182*+N195F, N54S+V56T+D183*+G182*+N195F, H1*+N54S+V56T+G109A+A174T+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116W+A174S+G182*+D183*+N195F+V206L+K391A+G476K, Q169E+Q172K+G182*+D183*+N195F, G182*+D183*+A186D, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238A+K391A+G476K, G182*+D183*+A186N, H1*+N54S+V56T+G109A+A174S+V206L+G182*+D183*+K391A+G476K, H1*+N54S+V56T+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206F+K391A+G476K, H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+A186N+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F289L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y295F+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S244Q+K391A+G476K, G182*+D183*+F328L, G182*+D183*+N195F+A288V, G182*+D183*+A186N+N195F, G182*+D183*+E190P+N195F, W140Y+G182*+D183*+N195F, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A225V+K391A+G476K, G182*+D183*+A186D+N195F, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+W48Y+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, G182*+D183*+N195F+V238A, G182*+D183*+Y295N, Q172G+G182*+D183*, F113Q+G182*+D183*+N195F, H1*+N54S+V56T+Q98A+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, G182*+D183*+N195F+Y295F, G182*+D183*+A186E, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206Y+K391A+G476K, G182*+D183*+N195F+W284H, G182*+D183*+A186Q+N195F, K72R+G182*+D183*, D183*+G182*+N195F+V326L, D183*+G182*+A186E+N195F, Q169E+G182*+D183*+N195F, D183*+G182*+N195F+K242Q, G7A+G182*+D183*+N195F, D183*+G182*+N195F+N260G, D183*+G182*+R321A+S323N, D183*+G182*+W284H, D183*+G182*+A186H+N195F, D183*+G182*+K391A, wherein numbering is according to SEQ ID NO: 13, V56T+G182*+D183*+N195F, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+A174S+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476K+G477S, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476G+G477T, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+G477Q, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+G477A, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476K+G477Q, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476N, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476Y, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V2061+1405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+A204G+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+A204G+V206L+G255A+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+R116H+T165G+Q172G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+Q172N+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+G196R+V206L+G255A+I405L+A421H+A422P+A428T+N475S, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+T40D+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+N125D+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+A60V+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+R116V+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+R116Q+G133Q+T165G+Q172G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+R116Q+Q172G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T+G448D, H1*+N54S+V56T+G109A+F113Q+R116H+Q172N+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+T134E+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+G255A+A265G+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+R116Q+W167F+Q172R+L173V+A174S+G182*+D183*+N195F+V206L+A265G+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+F113Q+T134E+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+G255A+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+G184T+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+G255A+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+N195F+A204G+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+A37H+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+A174S+M105F+G182*+D183*+N195F+V206L+L2281+R320A+S323N+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+F113Q+T134E+A174S+G182*+D183*+N195F+V206L+G255A+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+R116Q+V120L+A174S+G182*+D183*+G184T+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+R116Q+Q172N+A174S+G182*+D183*+N195F+V206L+A265G+I405L+A421H+A422P+A428T, H1*+A37H+N54S+V56T+A60V+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, wherein numbering is according to SEQ ID NO: 14.

In one embodiment, the IF is determined by use of Model J detergent at a temperature of 15° C. In a particular embodiment, the variants have an IF of >1.5 as compared to the parent polypeptide, wherein the IF is determined by use of Model J detergent at a temperature of 15° C. and a concentration of 0.05 mg/L. It can be seen from the Tables in the Examples herein disclosed which such variants may be.

Some variants according to the invention have an even further improved wash performance indicated by an IF of at least 2.0 when compared the a parent polypeptide having an amino acid sequence set forth in SEQ ID NO: 15, and wherein the IF is determined by use of Model A detergent at a temperature of 15° C.

Thus, in one embodiment, the variant has an IF of at least >2.0 when compared to said parent polypeptide, and wherein said variant is selected from H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+R87S+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T40G+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72H+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37H+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37M+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37Y+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37R+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113W+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q125P+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S381G+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246T+K391A+G476K, H1*+A37L+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q125A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377S+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377A+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S381A+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346P+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214S+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420Q+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420S+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420K+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420L+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391Y+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473G+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444D+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444Y+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280W+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280L+K391A+G476K, H1*+N54S+V56T+K72S+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T40K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346T+K391A+G476K, H1*+G50A+N54S+V56T+G109A++Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484S, H1*K+G50A+N54S+V56T+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+L173V+A174S+G182*+D183*+N195F+A204T+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W140Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346T+K391A+G476K+G477A, H1*+N54S+V56T+G109AG255A++A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+G50A+N54S+V56T+G109A+F113L+R116L+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320V+K391A+G476Y, H1*+N54S+V56T+G109A+R116M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L217V+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L217H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+P211D+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391Q+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444H+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444R+G476K, H1*+G50A+N54S+V56T+G109A+L173V+A174S+G182*+D183*+N195F+A204V+V206L+K391A+G476K, H1*+G7K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K, H1*+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+G346T+K391A+G476K+G477A, H1*+N54S+V56T+G109A+Q118S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y382M+K391A+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+K72R+M105F+G109A+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+M105F+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+S323N+D377H+K391A+G476K, H1*+T51E+N54S+V56T+G109A+A174H+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+L173A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Q299V+K391A+G476K, H1*+N54S+V56T+G109A+L173G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T355L+K391A+G476K, H1*+G50A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204T+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+R320A+S323N+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246L+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+W167H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+G109A+Q118R+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+Q172D+A174Q+G182*+D183*+N195F+V206L+K391A+G476K+G477A, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238T+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+D377H+K391A+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+G109A+L173H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72N+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72N+G109A+W167L+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+G184T+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q118R+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K,H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377S+K391A+G476K, H1*+N54S+V56T+G109A+R116E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+R116S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R1161+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51A+N54S+V56T+G109A+Q172S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51E+N54S+V56T+G109A+Q172T+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51S+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51E+N54S+V56T+G109A+Q172L+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51E+N54S+V56T+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q172S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72H+G109A+Q172T+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q172T+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72M+G109A+Q172S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72S+G109A+A174H+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+A174D+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174D+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+A174K+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174G+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174Q+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+A174G+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72S+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174H+G182*+D183*+N195F+V206L+K391A+G476K, H1*+G50A+N54S+V56T+G109A+A174E+G182*+D183*+N195F+A204T+V206L+S323N+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y3821+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214W+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204S+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T334S+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A263G+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M286F+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L235V+D377H+K391A+G476K, H1*+N54S+V56T+M105L+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+M105I+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+M105V+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M2461+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L2501+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250V+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391Y+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391M+G476K, H1*+N54S+V56T+G109A+T165G+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174V+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246F+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246V+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246S+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391R+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391H+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391W+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K3911+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250T+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250A+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250F+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250M+D377H+K391A+G476K, H1*+N54S+V56T+G109A+Q172H+L173A+A174P+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+G255A+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S376T+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S376V+K391A+G476K, H1*+N54S+V56T+G109A+Q172E+L1731+A174N+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172D+L173A+A174T+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267M+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238G +K391A+G476K, H1*+N54S+V56T+M105V+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V264T+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V2641+K391A+G476K, H1*+N54S+V56T+G109A+W167H+Q172N+L173A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172N+L173T+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172E+L173M+A174H+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W167Y+Q172E+L173V+A174H+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+L173A+A174T+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+F328L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250V+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250F+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250M+K391A+G476K, H1*+N54S+V56T+G109A+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51A+N54S+V56T+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391L+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109S+F113Q+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+A204G+V206L+G255A+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+W167F+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+G184T+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+P473G+G476K, H1*+N54S+V56T+G109A+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+M105F+G109A+A174S+G182*+D183*+N195F+V206L+R320A+S323N+K391A+C474V+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+G255A+K391A+C474V+G476K, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+C474V+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A265G+K391A+T444Q+G476K+G477A, H1*+A37V+N54S+V56T+G109A+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G346T+G477A+G476K, H1*+N54S+V56T+G109A+R116Q+Q172D+A174S+G182*+D183*+N195F+V206L+G346T+K391A+T444Q+G477A+G476K, H1*+N54S+V56T+G109A+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+G346T+K391A+T444Q+G477A+G476K, H1*+N54S+V56T+G109S+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109S+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+V2641+K391A+C474V+G476K, H1*+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280Q+H321Y+K391A+C474V+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+C474V+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109A+F113Q+R116Q+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+R116H+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+T165G+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116Q+T165G+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+F2891+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116Q+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116H+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+A265G+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+K72R+G109A+T134E+T165G+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+A265G+K391A+P473R+G476K, H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51V+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280Q+H321Y+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109A+R116Q+W167F+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+G133Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+Q395P+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+K72R+G109A+R116H+W167F+Q172R+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+G184T+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109S+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109S+R116Q+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109S+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+G109S+F113Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+R116H+T134E+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+K72R+G109A+R116H+T134E+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+G255A+K391A+Q395P+T444Q+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+T134E+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+G255A+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116H+T134E+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+G255A+K391A+Q395P+G476K, H1*+N54S+V56T+K72R+G109A+T134E+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+G255A+A265G+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+K72R+G109A+G133Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172N+L173V+A174S+G182*+D183*+G184T+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+A37H+N54S+V56T+G109A+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+T459N+G476K, H1*+N54S+V56T+G109A+F113Q+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+A60V+G109A+R116H+Q172R+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37H+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37H+N54S+V56T+A60V+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37H+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116A+W167F+Q172R+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+R116A+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+R116A+T165G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109A+G182*+D183*+N195F+K391A+G476K, H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L, N54S+V56T+G109A+G182*+D183*+N195F, N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+G476K, H1*+N54S+V56T+A174S+G182*+D183*+N195F+V206L+G273R+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L, N54S+V56T+G109A+A174S+G182*+D183*+N195F+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+R116Q+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+G109A+R116A+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+M246F+K391A+G476K, H1*+V56A+G109A+A174S+G182*+D183*+N195F+V206L+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116H+A174S+G182*+D183*+G184T+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109S+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+F113Q+R116Q+Q172N+A174S+G182*+D183*+N195F+V206L+A265G+K391A+P473R+G476K, H1*+N54S+V56T+G109A+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+R116A+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109A+R116H+Q172R+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K, H1*+A37V+N54S+V56T+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37H+N54S+V56T+G109A+R116H+T165G+A174S+G182*+D183*+N195F+V206L+M246F+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+K391A+G476K, H1*+N54S+V56T+G109A+G133S+A174S+G182*+D183*+N195F+K391A+G476H, H1*+N54S+V56T+G109A+R116A+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+G109A+R116H+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+P45A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+P364A+K391A+C474V+G476K, H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+G50A+T51A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V264F+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y382L+K391A+G476K, H1*+G7K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255A+Q256A+K391A+G476K, H1*+N54S+V56T+G109A+W167Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W167H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+T165V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174Q+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N16H+V17L+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y382F+K391A+G476K, H1*+Q32S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Q385L+K391A+G476K, H1*+N54S+V56T+R87S+G109A+R171H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116H+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+P473R+G476K, H1*+N54S+V56T+G109A+Q169E+Q172G+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+K391A+P473R+G476K, H1*+N54S+V56T+G109A+R116W+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206F+K391A+G476K, H1*+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N260G+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+Y295N+K391A+G476K, H1*+N54S+V56T+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+A186N+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+A288V+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+W48F+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+V291A+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+R320A+S323N+K391A+G476K, H1*+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116H+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N270T+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+N260G+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+A186D+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+A225V+K391A+G476K, H1*+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G255S+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K269Q+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255S+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, whereing numbering is according to SEQ ID NO: 13, and H1*+T40D+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T H1*+N54S+V56T+G109A+F113Q+N125D+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T H1*+N54S+V56T+G109A+F113Q+R116H+Q172N+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T H1*+N54S+V56T+K72R+G109A+T134E+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+G255A+A265G+I405L+A421H+A422P+A428T H1*+N54S+V56T+K72R+G109A+R116Q+W167F+Q172R+L173V+A174S+G182*+D183*+N195F+V206L+A265G+I405L+A421H+A422P+A428T H1*+N54S+V56T+K72R+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T H1*+N54S+V56T+K72R+G109A+F113Q+T134E+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+G255A+I405L+A421H+A422P+A428T H1*+N54S+V56T+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+N195F+A204G+V206L+I405L+A421H+A422P+A428T H1*+N54S+V56T+K72R+G109A+R116Q+V120L+A174S+G182*+D183*+G184T+N195F+V206L+I405L+A421H+A422P+A428T H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T H1*+N54S+V56T+G109A+F113Q+R116Q+Q172N+A174S+G182*+D183*+N195F+V206L+A265G+I405L+A421H+A422P+A428T H1*+A37H+N54S+V56T+A60V+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, wherein numbering is according to SEQ ID NO: 14, and wherein the IF is determined by use of Model A detergent.

In one embodiment, the variant has an IF of at least 2.0 when compared to the parent polypeptide, wherein the IF is determined by use of Model A detergent at a temperature of 15° C. and a concentration of 0.05 mg enzyme/L.

In one embodiment, the variant has an IF of at least 2.0 when compared to the parent polypeptide and when determined by use of Model J detergent, and wherein the variant is selected from the group consisting of: H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+R87S+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T40G+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72H+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37H+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37M+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37Y+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37R+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113W+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q118N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q125P+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q125K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S381G+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246T+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V213T+K391A+G476K, H1*+A37L+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q125A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377S+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377G+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377R+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377A+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214G+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S381A+K391A+G476K, H1*+N54S+V56T+G109A+W1671+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W167G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346S+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346P+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214S+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214T+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420S+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420K+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420L+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391Y+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391R+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473G+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473T+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+Q395P+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444S+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444D+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444Y+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280W+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280T+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280A+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255N+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T285L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T285Q+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473K+G476K, H1*+N54S+V56T+K72S+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T40K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346T+K391A+G476K, H1*+G50A+N54S+V56T+G109A++Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484A, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484G, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484P, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484E, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484Q, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484S, H1*K+G50A+N54S+V56T+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+L173V+A174S+G182*+D183*+N195F+A204T+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W140Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+Q98S+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+H324L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346T+K391A+G476K+G477A, H1*+N54S+V56T+G109AG255A++A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+G50A+N54S+V56T+G109A+F113L+R116L+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+G477Q, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Q345D+K391A+G476K+G477Q, H1*+G50A+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V2641+K391A+G423H+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320V+K391A+G476Y, H1*+N54S+V56T+G109A+R116M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L217V+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L217H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+P211D+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391Q+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444H+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444V+G476K, H1*+G50A+N54S+V56T+G109A+L173V+A174S+G182*+D183*+N195F+A204V+V206L+K391A+G476K, H1*+G50A+N54S+V56T+G109A+F113L+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+G7K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K H1*+N54S+V56T+G109A+Q118G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+G346T+K391A+G476K+G477A, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y382M+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+K72R+M105F+G109A+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+M105F+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+S323N+D377H+K391A+G476K, H1*+T51E+N54S+V56T+G109A+A174H+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+L173A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Q299V+K391A+G476K, H1*+N54S+V56T+G109A+L173G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T355L+K391A+G476K, H1*+G50A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204T+V206L+K391A+G476K, H1*+G50A+N54S+V56T+G109A+A174E+G182*+D183*+N195F+A204T+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+R320A+S323N+K391A+G476K, H1*+T51G+N54S+V56T+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+Q11H+N54S+V56T+G109A+Q118R+T134E+A174S+G184T+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+G109A+Q118R+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+Q172D+A174Q+G182*+D183*+N195F+V206L+K391A+G476K+G477A, H1*+N54S+V56T+K72R+G109A+W167L+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K, H1*+N54S+V56T+M105F+G109A+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+R320A+S323N+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291G+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+F328L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238A+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238T+D377H+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238G+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F328M+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F3281+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291T+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+D377H+K391A+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+G109A+L173H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q125T+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N16S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72N+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+G184T+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q118R+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+R320A+S323N+K391A+G476K, H1*+N54S+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L217T+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377S+K391A+G476K, H1*+N54S+V56T+G109A+R116E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377A+K391A+G476K, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346P+K391A+G476K, H1*+N54S+V56T+G109A+R116S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377Q+K391A+G476K, H1*+N54S+V56T+G109A+R1161+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+T51A+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51S+N54S+V56T+G109A+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*-+T51A+N54S+V56T+G109A+Q172S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51E+N54S+V56T+G109A+Q172T+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51A+N54S+V56T+G109A+Q172A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51S+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51A+N54S+V56T+G109A+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51E+N54S+V56T+G109A+Q172L+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q172S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72H+G109A+Q172T+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q172M+A174S+G182*+D183*+N195F+V206L+Y295N+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q172T+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72M+G109A+Q172S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72S+G109A+A174H+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+A174D+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174D+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+A174K+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174G+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174Q+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+A174G+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72S+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174H+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444A+G476K, H1*+N54S+V56T+G109A+F113L+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+M105F+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S376H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214E+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214W+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204T+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204S+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+T165S+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T334S+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A263G+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M286F+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L235V+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L235A+D377H+K391A+G476K, H1*+N54S+V56T+M105L+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+M105I+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+M105V+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M2461+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L2501+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250V+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391Y+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391M+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391E+G476K, H1*+N54S+V56T+G109A+T165G+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174V+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246F+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174L+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206S+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391D+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391R+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391H+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391W+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K3911+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250T+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250A+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250F+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250M+D377H+K391A+G476K, H1*+N54S+V56T+G109A+Q172H+L173A+A174P+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+G255A+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S376T+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S376V+K391A+G476K, H1*+N54S+V56T+G109A+Q172E+L1731+A174N+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172D+L173A+A174T+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267M+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267H+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238A+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238G +K391A+G476K, H1*+N54S+V56T+M105V+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V264T+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V2641+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L235V+K391A+G476K, H1*+N54S+V56T+G109A+W167H+Q172N+L173A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W167F+Q172E+L173P+A174K+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172N+L173T+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172E+L173F+A174R+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172E+L173T+A174R+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172N+L173V+A174R+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172E+L173M+A174H+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172E+L173F+A174P+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W167Y+Q172E+L173V+A174H+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+L173A+A174T+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+F328L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246S+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250V+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250F+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250M+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T334S+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A263G+M286L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391L+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109S+F113Q+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+A204G+V206L+G255A+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+W167F+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+G184T+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+P473G+G476K, H1*+N54S+V56T+G109A+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+M105F+G109A+A174S+G182*+D183*+N195F+V206L+R320A+S323N+K391A+C474V+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+G255A+K391A+C474V+G476K, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+C474V+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A265G+K391A+T444Q+G476K+G477A, H1*+A37V+N54S+V56T+G109A+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G346T+G477A+G476K, H1*+N54S+V56T+G109A+R116Q+Q172D+A174S+G182*+D183*+N195F+V206L+G346T+K391A+T444Q+G477A+G476K, H1*+N54S+V56T+G109A+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+G346T+K391A+T444Q+G477A+G476K, H1*+N54S+V56T+G109S+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109S+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109S+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+V2641+K391A+C474V+G476K, H1*+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280Q+H321Y+K391A+C474V+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+C474V+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109A+F113Q+R116Q+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+R116H+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+T165G+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116Q+T165G+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+F2891+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116Q+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116H+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+T165G+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+Q395P+G476K, H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+A265G+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+K72R+G109A+T134E+T165G+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+A265G+K391A+P473R+G476K, H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51V+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280Q+H321Y+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109A+R116Q+W167F+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+G133Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+Q395P+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+K72R+G109A+R116H+W167F+Q172R+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+G184T+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109S+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109S+R116Q+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109S+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+G109S+F113Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+R116H+T134E+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+K72R+G109A+R116H+T134E+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+G255A+K391A+Q395P+T444Q+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+T134E+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+G255A+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+T134E+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+G255A+K391A+Q395P+A445Q+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116H+T134E+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+G255A+K391A+Q395P+G476K, H1*+N54S+V56T+K72R+G109A+T134E+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+G255A+A265G+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+K72R+G109A+G133Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172N+L173V+A174S+G182*+D183*+G184T+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+A37H+N54S+V56T+G109A+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37H+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+T459N+G476K, H1*+N54S+V56T+G109A+F113Q+Q172R+L173V+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+A60V+G109A+R116H+Q172R+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37H+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37H+N54S+V56T+A60V+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37H+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116A+W167F+Q172R+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+R116A+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+R116A+T165G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109A+G182*+D183*+N195F+K391A+G476K, H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L, N54S+V56T+G109A+G182*+D183*+N195F, N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+G476K, H1*+N54S+V56T+A174S+G182*+D183*+N195F+V206L+G273R+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L, H1*+W48G+G109A+G182*+D183*+N195F+V206L+K391A+G476K, N54S+V56T+G109A+A174S+G182*+D183*+N195F+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+K391A, H1*+N54S+V56T+G109A+F113Q+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+R116Q+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+G109A+R116A+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+M246F+K391A+G476K, H1*+V56A+G109A+A174S+G182*+D183*+N195F+V206L+G476K, H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+K391A, H1*+N54S+V56T+K72R+G109A+F113Q+R116H+A174S+G182*+D183*+G184T+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109S+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+F113Q+R116Q+Q172N+A174S+G182*+D183*+N195F+V206L+A265G+K391A+P473R+G476K, H1*+N54S+V56T+G109A+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+R116A+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109A+R116H+Q172R+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K, H1*+A37V+N54S+V56T+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37H+N54S+V56T+G109A+R116H+T165G+A174S+G182*+D183*+N195F+V206L+M246F+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+K391A+G476K, G109A+G182*+D183*+N195F+V206L+G476K, H1*+N54S+V56T+G109A+G133S+A174S+G182*+D183*+N195F+K391A+G476H, H1*+N54S+V56T+G109A+R116A+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+G109A+R116H+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+P45A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+P124A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+P364A+K391A+C474V+G476K, H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+G50A+T51A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V264F+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y382L+K391A+G476K, H1*+G7K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255A+Q256A+K391A+G476K, H1*+N54S+V56T+G109A+W167Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W167H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+T165V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174Q+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N16H+V17L+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y382F+K391A+G476K, H1*+Q32S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Q385L+K391A+G476K, H1*+N54S+V56T+R87S+G109A+R171H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116H+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+P473R+G476K, H1*+N54S+V56T+G109A+Q169E+Q172G+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+K391A+P473R+G476K, H1*+N54S+V56T+G109A+R116W+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206F+K391A+G476K, H1*+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N260G+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+Y295N+K391A+G476K, H1*+N54S+V56T+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+A186N+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+A288V+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+W48F+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+V291A+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+R320A+S323N+K391A+G476K, H1*+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116H+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N270T+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+N260G+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+A186D+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+A225V+K391A+G476K, H1*+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G255S+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K269Q+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255S+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, G182*+D183*+N195F, H1*+N54S+V56T+G109A+Q169E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+W284H+K391A+G476K, H1*+N54S+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+S304R+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246V+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+N270G+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+Y295F+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174T+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W140Y+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+F328L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G476K, F113Q+R116H+D183*+G182*+N195F, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A288L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N270G-FK391A+G476K, Q169E+Q172K+A174*+D183*+G182*+N195F, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K269Q+K391A+G476K, H1*+W48F+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, F113Q+R116Q+D183*+G182*+N195F, N54S+V56T+D183*+G182*+N195F, H1*+N54S+V56T+G109A+A174T+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116W+A174S+G182*+D183*+N195F+V206L+K391A+G476K, Q169E+Q172K+G182*+D183*+N195F, G182*+D183*+A186D, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238A+K391A+G476K, G182*+D183*+A186N, wherein numbering is according to SEQ ID NO: 13, H1*+N54S+V56T+G109A+A1745+G182*+D183*+N195F+V2061+1405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+A204G+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+A204G+V206L+G255A+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+R116H+T165G+Q172G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+G196R+V206L+G255A+I405L+A421H+A422P+A428T+N475S, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+N125D+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+R116V+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+R116Q+G133Q+T165G+Q172G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+R116Q+Q172G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T+G448D, H1*+N54S+V56T+G109A+F113Q+R116H+Q172N+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+T134E+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+G255A+A265G+I405L+A421 H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+R116Q+W167F+Q172R+L173V+A174S+G182*+D183*+N195F+V206L+A265G+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+F113Q+T134E+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+G255A+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+G255A+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+R116Q+V120L+A174S+G182*+D183*+G184T+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+R116Q+Q172N+A174S+G182*+D183*+N195F+V206L+A265G+I405L+A421H+A422P+A428T, H1*+A37H+N54S+V56T+A60V+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, wherein numbering is according to SEQ ID NO: 14.

In one embodiment, the variant has an IF of at least 2.0 when compared to the parent polypeptide, wherein the IF is determined by use of Model J detergent at a temperature of 15° C. and an enzymes concentration of 0.05 mg/L.

Other variants according to the invention have an IF of at least 3.0, indicating that at least these variants have a significantly improved wash performance when compared the a parent polypeptide having an amino acid sequence set forth in SEQ ID NO: 15.

In a particular embodiment, the variant is selected from a group consisting of: H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+R116Q+W167F+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116Q+Q172N+A174S+G182*+D183*+N195F+V206L+A265G+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+G133Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+R116H+T134E+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, and H1*+N54S+V56T+K72R+G109A+R116H+T134E+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+G255A+K391A+Q395P+T444Q+P473R+G476K, wherein numbering is according to SEQ ID NO: 13.

In another embodiment, the variant has an IF of at least 3.0 when compared to the parent polypeptide and determined by use of Model J detergent, and wherein the variant is selected from the group consisting of: H1*+T51K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q125A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444D+G476K, H1*+N54S+V56T+K72S+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+G50A+N54S+V56T+G109A+L173V+A174S+G182*+D183*+N195F+A204V+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+G346T+K391A+G476K+G477A, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+Q172D+A174Q+G182*+D183*+N195F+V206L+K391A+G476K+G477A, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+G184T+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238G +K391A+G476K, H1*+N54S+V56T+G109A+W167F+Q172E+L173P+A174K+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250M+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109S+F113Q+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+A204G+V206L+G255A+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+W167F+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K, H1*+N54S+V56T+M105F+G109A+A174S+G182*+D183*+N195F+V206L+R320A+S323N+K391A+C474V+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+G255A+K391A+C474V+G476K, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+C474V+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+R116Q+Q172D+A174S+G182*+D183*+N195F+V206L+G346T+K391A+T444Q+G477A+G476K, H1*+N54S+V56T+G109S+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109S+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+V2641+K391A+C474V+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+C474V+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109A+F113Q+R116Q+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+T165G+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116Q+T165G+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+T134E+T165G+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116Q+W167F+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+G133Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+Q395P+K391A+G476K, H1*+A37V+N54S+V56T+K72R+G109A+R116H+W167F+Q172R+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+G184T+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109S+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109S+R116Q+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109S+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+G109S+F113Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+R116H+T134E+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+G255A+K391A+Q395P+T444Q+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+T134E+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+G255A+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+K72R+G109A+T134E+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+G255A+A265G+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+K72R+G109A+G133Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+A60V+G109A+R116H+Q172R+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37H+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37H+N54S+V56T+A60V+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37H+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116A+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+R116Q+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+G109A+R116A+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+M246F+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116H+A174S+G182*+D183*+G184T+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109S+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+F113Q+R116Q+Q172N+A174S+G182*+D183*+N195F+V206L+A265G+K391A+P473R+G476K, H1*+A37V+N54S+V56T+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37H+N54S+V56T+G109A+R116H+T165G+A174S+G182*+D183*+N195F+V206L+M246F+K391A+G476K, H1*+N54S+V56T+G109A+R116A+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+P45A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+G50A+T51A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V264F+K391A+G476K, H1*+G7K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255A+Q256A+K391A+G476K, H1*+N54S+V56T+G109A+W167Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N16H+V17L+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y382F+K391A+G476K, H1*+Q32S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Q385L+K391A+G476K, H1*+N54S+V56T+R87S+G109A+R171H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116H+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+P473R+G476K, H1*+N54S+V56T+G109A+Q169E+Q172G+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+K391A+P473R+G476K, H1*+N54S+V56T+G109A+R116W+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206F+K391A+G476K, H1*+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N260G+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+Y295N+K391A+G476K, H1*+N54S+V56T+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+A186N+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+A288V+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+W48F+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+V291A+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+R320A+S323N+K391A+G476K, H1*+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116H+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N270T+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+N260G+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, wherein numbering is according to SEQ ID NO: 13, H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+R116Q+Q172N+A174S+G182*+D183*+N195F+V206L+A265G+I405L+A421H+A422P+A428T, wherein numbering is according to SEQ ID NO: 14.

The variants according to the present invention may further comprise one or more additional alterations than those described above at one or more (e.g., several) other positions.

The amino acid changes may be of a minor nature, that is conservative amino acid substitutions or insertions that do not significantly affect the folding and/or activity of the protein; small deletions, typically of 1-30 amino acids; small amino- or carboxyl-terminal extensions, such as an amino-terminal methionine residue; a small linker peptide of up to 20-25 residues; or a small extension that facilitates purification by changing net charge or another function, such as a poly-histidine tract, an antigenic epitope or a binding domain.

Examples of conservative substitutions are within the groups of basic amino acids (arginine, lysine and histidine), acidic amino acids (glutamic acid and aspartic acid), polar amino acids (glutamine and asparagine), hydrophobic amino acids (leucine, isoleucine and valine), aromatic amino acids (phenylalanine, tryptophan and tyrosine), and small amino acids (glycine, alanine, serine, threonine and methionine). Amino acid substitutions that do not generally alter specific activity are known in the art and are described, for example, by H. Neurath and R. L. Hill, 1979, In, The Proteins, Academic Press, New York. Common substitutions are Ala/Ser, Val/Ile, Asp/Glu, Thr/Ser, Ala/Gly, Ala/Thr, Ser/Asn, AlaNal, Ser/Gly, Tyr/Phe, Ala/Pro, Lys/Arg, Asp/Asn, Leu/Ile, Leu/Val, Ala/Glu, and Asp/Gly.

Alternatively, the amino acid changes are of such a nature that the physico-chemical properties of the polypeptides are altered. For example, amino acid changes may improve the thermal stability of the polypeptide, alter the substrate specificity, change the pH optimum, and the like.

Essential amino acids in a polypeptide may be identified according to procedures known in the art, such as site-directed mutagenesis or alanine-scanning mutagenesis (Cunningham and Wells, 1989, Science 244: 1081-1085). In the latter technique, single alanine mutations are introduced at every residue in the molecule, and the resultant mutant molecules are tested for alpha-amylase activity to identify amino acid residues that are critical to the activity of the molecule. See also, Hilton et al., 1996, J. Biol. Chem. 271: 4699-4708. The active site of the enzyme or other biological interaction can also be determined by physical analysis of structure, as determined by such techniques as nuclear magnetic resonance, crystallography, electron diffraction, or photoaffinity labeling, in conjunction with mutation of putative contact site amino acids. See, for example, de Vos et al., 1992, Science 255: 306-312; Smith et al., 1992, J. Mol. Biol. 224: 899-904; Wlodaver et al., 1992, FEBS Lett. 309: 59-64. The identity of essential amino acids can also be inferred from an alignment with a related polypeptide.

The variants according to the present invention may consist of 400 to 485 amino acids, e.g., 410 to 485, 425 to 485, and 440 to 485 amino acids.

Polynucleotides

The present invention also relates to polynucleotides encoding a variant of the present invention. Thus, in particular, the present invention relates to a polynucleotide endocing a variant comprising an alteration in one or more positions corresponding to the H1, T5, G7, Q11, N16, V17, Q32, A37, T40, P45, W48, G50, T51, N54, V56, K72, R87, Q98, M105, G109, F113, R116, Q118, Q125, G133, T134, W140, G142, G149, T165, W167, Q169, R171, Q172, L173, A174, G184, A186, E190, T193, N195, A204, V206, P211, 1214, V215, L217, L219, A225, L235, V238, K242, S244, M246, M248, L250, G255, Q256, N260, A263, V264, Y267, K269, N270, G273, S280, W284, T285, M286, F289, V291, Y295, Q299, R320, H321, S323, H324, V326, F328, T334, D337, Q345, G346, G348, T355, S376, D377, D379, Y382, S383, Q385, K391, K393, Q395, A420, G423, T444, A445, Q449, T459, P473, C474, G476, G477, and K484 of SEQ ID NO: 13; or H1, A37, T40, N54, V56, A60, K72, G109, F113, R116, N125, F133, T134, T165, Q169, Q172, L173, A174, G184, A186, E190, N195, G196, A204, V206, A225, L228, K242, S244, G255, N260, A265, K269, S280, W284, S304, R320, H321, S323, V326, K391, I405, A421, A422, A428, G448, D476, and G477 of SEQ ID NO: 14.

The term “polynucleotides encoding” as used herein, refers to a polynucleotide that encodes a mature polypeptide having alpha-amylase having alpha-amylase activity. In one aspect, the polypeptide coding sequence is the nucleotide sequence set forth in SEQ ID NO: 1, 2, or 3.

In one embodiment, the polynucleotide encoding a variant according to the present invention as at least 60%, such as at least 65%, such as at least 70%, such as at least 75%, such as at least 80%, such as at least 85%, such as at least 90%, such as at least 95%, such as at least 96%, such as at least 97%, such as at least 98%, such as at least 99% but less than 100% sequence identity to the polynucleotide of SEQ ID NOs: 1, 2 and 3.

Nucleic Acid Constructs

The present invention also relates to nucleic acid constructs comprising a polynucleotide encoding a variant of the present invention operably linked to one or more control sequences that direct the expression of the coding sequence in a suitable host cell under conditions compatible with the control sequences. Thus, in particular, the present invention relates to a nucleic acid construct comprising a polynucleotide encoding a variant comprising an alteration in one or more positions corresponding to the positions H1, T5, G7, Q11, N16, V17, Q32, A37, T40, P45, W48, G50, T51, N54, V56, K72, R87, Q98, M105, G109, F113, R116, Q118, Q125, G133, T134, W140, G142, G149, T165, W167, Q169, R171, Q172, L173, A174, G184, A186, E190, T193, N195, A204, V206, P211, 1214, V215, L217, L219, A225, L235, V238, K242, S244, M246, M248, L250, G255, Q256, N260, A263, V264, Y267, K269, N270, G273, S280, W284, T285, M286, F289, V291, Y295, Q299, R320, H321, S323, H324, V326, F328, T334, D337, Q345, G346, G348, T355, S376, D377, D379, Y382, S383, Q385, K391, K393, Q395, A420, G423, T444, A445, Q449, T459, P473, C474, G476, G477, and K484 of SEQ ID NO: 13; or H1, A37, T40, N54, V56, A60, K72, G109, F113, R116, N125, F133, T134, T165, Q169, Q172, L173, A174, G184, A186, N195, G196, A204, V206, A225, L228, K242, S244, G255, N260, A265, K269, S280, W284, Y295, S304, R320, H321, S323, V326, K391, 1405, A421, A422, A428, R439, G448, W467, D476, and G477 of SEQ ID NO: 14, wherein the polynucleotide is operately linked to one or more control sequences.

The term “nucleic acid construct” as used herein, refers to a nucleic acid molecule, either single- or double-stranded, which is isolated from a naturally occurring gene or is modified to contain segments of nucleic acids in a manner that would not otherwise exist in nature or which is synthetic, which comprises one or more control sequences.

The term “operably linked” as used herein, refers to a configuration in which a control sequence is placed at an appropriate position relative to the coding sequence of a polynucleotide such that the control sequence directs expression of the coding sequence.

The polynucleotide may be manipulated in a variety of ways to provide for expression of a variant. Manipulation of the polynucleotide prior to its insertion into a vector may be desirable or necessary depending on the expression vector. The techniques for modifying polynucleotides utilizing recombinant DNA methods are well known in the art.

The control sequence may be a promoter, a polynucleotide which is recognized by a host cell for expression of the polynucleotide. The promoter comprises transcriptional control sequences that mediate the expression of the variant. The promoter may be any polynucleotide that shows transcriptional activity in the host cell including mutant, truncated, and hybrid promoters, and may be obtained from genes encoding extracellular or intracellular polypeptides either homologous or heterologous to the host cell.

Expression Vectors

The present invention also relates to recombinant expression vectors comprising a polynucleotide encoding a variant of the present invention, a promoter, and transcriptional and translational stop signals. Thus, the present invention relates to an expression vector, optionally recombinant, comprising a polynucleotide encoding a variant comprising an alteration in one or more positions corresponding to the positions H1, T5, G7, Q11, N16, V17, Q32, A37, T40, P45, W48, G50, T51, N54, V56, K72, R87, Q98, M105, G109, F113, R116, Q118, Q125, G133, T134, W140, G142, G149, T165, W167, Q169, R171, Q172, L173, A174, G184, A186, E190, T193, N195, A204, V206, P211, 1214, V215, L217, L219, A225, L235, V238, K242, S244, M246, M248, L250, G255, Q256, N260, A263, V264, Y267, K269, N270, G273, S280, W284, T285, M286, F289, V291, Y295, Q299, R320, H321, S323, H324, V326, F328, T334, D337, Q345, G346, G348, T355, S376, D377, D379, Y382, S383, Q385, K391, K393, Q395, A420, G423, T444, A445, Q449, T459, P473, C474, G476, G477, and K484 of SEQ ID NO: 13; or H1, A37, T40, N54, V56, A60, K72, G109, F113, R116, N125, F133, T134, T165, Q169, Q172, L173, A174, G184, A186, N195, G196, A204, V206, A225, L228, K242, S244, G255, N260, A265, K269, S280, W284, Y295, S304, R320, H321, S323, V326, K391, 1405, A421, A422, A428, R439, G448, W467, D476, and G477 of SEQ ID NO: 14, a promotor, and transcriptional and translational stop signals.

The term “expression vector” as used herein, refers to a linear or circular DNA molecule that comprises a polynucleotide encoding a variant and is operably linked to control sequences that provide for its expression.

The various nucleotide and control sequences may be joined together to produce a recombinant expression vector that may include one or more convenient restriction sites to allow for insertion or substitution of the polynucleotide encoding the variant at such sites. Alternatively, the polynucleotide may be expressed by inserting the polynucleotide or a nucleic acid construct comprising the polynucleotide into an appropriate vector for expression. In creating the expression vector, the coding sequence is located in the vector so that the coding sequence is operably linked with the appropriate control sequences for expression.

The recombinant expression vector may be any vector (e.g., a plasmid or virus) that can be conveniently subjected to recombinant DNA procedures and can bring about expression of the polynucleotide. The choice of the vector will typically depend on the compatibility of the vector with the host cell into which the vector is to be introduced. The vector may be a linear or closed circular plasmid.

The vector may be an autonomously replicating vector, i.e., a vector that exists as an extrachromosomal entity, the replication of which is independent of chromosomal replication, e.g., a plasmid, an extrachromosomal element, a minichromosome, or an artificial chromosome. The vector may contain any means for assuring self-replication. Alternatively, the vector may be one that, when introduced into the host cell, is integrated into the genome and replicated together with the chromosome(s) into which it has been integrated. Furthermore, a single vector or plasmid or two or more vectors or plasmids that together contain the total DNA to be introduced into the genome of the host cell, or a transposon, may be used.

The vector preferably contains one or more selectable markers that permit easy selection of transformed, transfected, transduced, or the like cells. A selectable marker is a gene the product of which provides for biocide or viral resistance, resistance to heavy metals, prototrophy to auxotrophs, and the like.

The skilled person would know which expression vector is the most suitable for specific expression systems. Thus, the present invention is not limited to any specific expression vector, but any expression vector comprising the polynucleotide encoding a variant according to the invention is considered part of the present invention.

Host Cells

The present invention also relates to recombinant host cells, comprising a polynucleotide encoding a variant of the present invention operably linked to one or more control sequences that direct the production of a variant of the present invention. Thus, the present invention relates to a host cell, optionally a recombinant host cell, comprising a polynecleotide encoding a variant comprising an alteration in one or more positions corresponding to the positions H1, T5, G7, Q11, N16, V17, Q32, A37, T40, P45, W48, G50, T51, N54, V56, K72, R87, Q98, M105, G109, F113, R116, Q118, Q125, G133, T134, W140, G142, G149, T165, W167, Q169, R171, Q172, L173, A174, G184, A186, E190, T193, N195, A204, V206, P211, 1214, V215, L217, L219, A225, L235, V238, K242, S244, M246, M248, L250, G255, Q256, N260, A263, V264, Y267, K269, N270, G273, S280, W284, T285, M286, F289, V291, Y295, Q299, R320, H321, S323, H324, V326, F328, T334, D337, Q345, G346, G348, T355, S376, D377, D379, Y382, S383, Q385, K391, K393, Q395, A420, G423, T444, A445, Q449, T459, P473, C474, G476, G477, and K484 of SEQ ID NO: 13; or H1, A37, T40, N54, V56, A60, K72, G109, F113, R116, N125, F133, T134, T165, Q169, Q172, L173, A174, G184, A186, E190, N195, G196, A204, V206, A225, L228, K242, S244, G255, N260, A265, K269, S280, W284, Y295, S304, R320, H321, S323, V326, K391, 1405, A421, A422, A428, R439, G448, W467, D476, and G477 of SEQ ID NO: 14, operably linked to one or more control sequences that direct the production of the variant.

The term “host cell” as used herein, refers to any cell type that is susceptible to transformation, transfection, transduction, or the like with a nucleic acid construct or expression vector comprising a polynucleotide of the present invention. The term “host cell” encompasses any progeny of a parent cell that is not identical to the parent cell due to mutations that occur during replication.

A construct or vector comprising a polynucleotide is introduced into a host cell so that the construct or vector is maintained as a chromosomal intearant or as a self-replicating extra-chromosomal vector as described earlier. The choice of a host cell will to a large extent depend upon the gene encoding the variant and its source.

The host cell may be any cell useful in the recombinant production of a variant, e.g., a prokaryote or a eukaryote.

The prokaryotic host cell may be any Gram-positive or Gram-negative bacterium. Gram-positive bacteria include, but are not limited to, Bacillus, Clostridium, Enterococcus, Geobacillus, Lactobacillus, Lactococcus, Oceanobacillus, Staphylococcus, Streptococcus, and Streptomyces. Gram-negative bacteria include, but are not limited to, Campylobacter, E. coli, Flavobacterium, Fusobacterium, Helicobacter, Ilyobacter, Neisseria, Pseudomonas, Salmonella, and Ureaplasma.

Methods According to the Invention

The present invention also relates to methods of producing a variant, comprising: (a) cultivating a host cell of the present invention under conditions suitable for expression of the variant; and (b) recovering the variant. Thus, the present invention relates to a method of producing a variant comprising an alteration in one or more positions corresponding to the positions H1, T5, G7, Q11, N16, V17, Q32, A37, T40, P45, W48, G50, T51, N54, V56, K72, R87, Q98, M105, G109, F113, R116, Q118, Q125, G133, T134, W140, G142, G149, T165, W167, Q169, R171, Q172, L173, A174, G184, A186, E190, T193, N195, A204, V206, P211, I214, V215, L217, L219, A225, L235, V238, K242, S244, M246, M248, L250, G255, Q256, N260, A263, V264, Y267, K269, N270, G273, S280, W284, T285, M286, F289, V291, Y295, Q299, R320, H321, S323, H324, V326, F328, T334, D337, Q345, G346, G348, T355, S376, D377, D379, Y382, S383, Q385, K391, K393, Q395, A420, G423, T444, A445, Q449, T459, P473, C474, G476, G477, and K484 of SEQ ID NO: 13; or H1, A37, T40, N54, V56, A60, K72, G109, F113, R116, N125, F133, T134, T165, Q169, Q172, L173, A174, G184, A186, E190, N195, G196, A204, V206, A225, L228, K242, S244, G255, N260, A265, K269, S280, W284, Y295, S304, R320, H321, S323, V326, K391, 1405, A421, A422, A428, R439, G448, W467, D476, and G477 of SEQ ID NO: 14, wherein the method comprises the steps of a) cultivating a host cell according to the invention under conditions suitable for expression of the variant, and b) recovering the variant.

The host cells are cultivated in a nutrient medium suitable for production of the variant using methods known in the art. For example, the cell may be cultivated by shake flask cultivation, or small-scale or large-scale fermentation (including continuous, batch, fed-batch, or solid state fermentations) in laboratory or industrial fermentors performed in a suitable medium and under conditions allowing the variant to be expressed and/or isolated. The cultivation takes place in a suitable nutrient medium comprising carbon and nitrogen sources and inorganic salts, using procedures known in the art. Suitable media are available from commercial suppliers or may be prepared according to published compositions (e.g., in catalogues of the American Type Culture Collection). If the variant is secreted into the nutrient medium, the variant can be recovered directly from the medium. If the variant is not secreted, it can be recovered from cell lysates.

The variant may be detected using methods known in the art that are specific for the polypeptides having alpha amylase activity. These detection methods include, but are not limited to, use of specific antibodies, formation of an enzyme product, or disappearance of an enzyme substrate. For example, an enzyme assay may be used to determine the activity of the variant.

The variant may be recovered using methods known in the art. For example, the variant may be recovered from the nutrient medium by conventional procedures including, but not limited to, collection, centrifugation, filtration, extraction, spray-drying, evaporation, or precipitation.

The variant may be purified by a variety of procedures known in the art including, but not limited to, chromatography (e.g., ion exchange, affinity, hydrophobic, chromatofocusing, and size exclusion), electrophoretic procedures (e.g., preparative isoelectric focusing), differential solubility (e.g., ammonium sulfate precipitation), SDS-PAGE, or extraction (see, e.g., Protein Purification, Janson and Ryden, editors, VCH Publishers, New York, 1989) to obtain substantially pure variants.

In an alternative aspect, the variant is not recovered, but rather a host cell of the present invention expressing the variant is used as a source of the variant.

Furthermore, the present invention relates to methods for obtaining a variant, comprising introducing into a parent alpha-amylase having at least 80% sequence identity to the polypeptide of SEQ ID NO: 4

(a) a substitution and/or deletion of two or more positions in the parent alpha-amylase said positions corresponding to positions 181, 182, 183, and 184 of the mature polypetide of SEQ ID NO: 4; and

(b) an alteration at one or more positions corresponding to positions H1, T5, G7, Q11, N16, V17, Q32, A37, T40, P45, W48, G50, T51, N54, V56, K72, R87, Q98, M105, G109, F113, R116, Q118, Q125, G133, T134, W140, G142, G149, T165, W167, Q169, R171, Q172, L173, A174, G184, A186, E190, T193, N195, A204, V206, P211,1214, V215, L217, L219, A225, L235, V238, K242, S244, M246, M248, L250, G255, Q256, N260, A263, V264, Y267, K269, N270, G273, S280, W284, T285, M286, F289, V291, Y295, Q299, R320, H321, S323, H324, V326, F328, T334, D337, Q345, G346, G348, T355, S376, D377, D379, Y382, S383, Q385, K391, K393, Q395, A420, G423, T444, A445, Q449, T459, P473, C474, G476, G477, and K484 of SEQ ID NO: 13; or H1, A37, T40, N54, V56, A60, K72, G109, F113, R116, N125, F133, T134, T165, Q169, Q172, L173, A174, G184, A186, E190, N195, G196, A204, V206, A225, L228, K242, S244, G255, N260, A265, K269, S280, W284, Y295, S304, R320, H321, S323, V326, K391, I405, A421, A422, A428, R439, G448, W467, D476, and G477 of SEQ ID NO: 14,

wherein the resulting variant has at least 80%, such as at least 85%, such as at least 90%, such as at least 95%, such as at least 97%, but less than 100% sequence identity with the mature polypeptide of SEQ ID NO: 4, wherein said variant has alpha-amylase activity;

The variants may be prepared using any mutagenesis procedure known in the art, such as site-directed mutagenesis, synthetic gene construction, semi-synthetic gene construction, random mutagenesis, shuffling, etc.

Site-directed mutagenesis is a technique in which one or more (e.g., several) mutations are introduced at one or more defined sites in a polynucleotide encoding the parent.

Site-directed mutagenesis can be accomplished in vitro by PCR involving the use of oligonucleotide primers containing the desired mutation. Site-directed mutagenesis can also be performed in vitro by cassette mutagenesis involving the cleavage by a restriction enzyme at a site in the plasmid comprising a polynucleotide encoding the parent polypeptide and subsequent ligation of an oligonucleotide containing the mutation in the polynucleotide. Usually the restriction enzyme that digests the plasmid and the oligonucleotide is the same, permitting sticky ends of the plasmid and the insert to ligate to one another. See, e.g., Scherer and Davis, 1979, Proc. Natl. Acad. Sci. USA 76: 4949-4955; and Barton et al., 1990, Nucleic Acids Res. 18: 7349-4966.

Site-directed mutagenesis can also be accomplished in vivo by methods known in the art. See, e.g., U.S. Patent Application Publication No. 2004/0171154; Storici et al., 2001, Nature Biotechnol. 19: 773-776; Kren et al., 1998, Nat. Med. 4: 285-290; and Calissano and Macino, 1996, Fungal Genet. Newslett. 43: 15-16.

Any site-directed mutagenesis procedure may be used in the present invention. There are many commercial kits available that can be used to prepare variants. The skilled person in the art is well-aware of such commercial kits and how to use them.

Synthetic gene construction entails in vitro synthesis of a designed polynucleotide molecule to encode a polypeptide of interest. Gene synthesis may be performed utilizing a number of techniques, such as the multiplex microchip-based technology described by Tian et al. (2004, Nature 432: 1050-1054) and similar technologies wherein oligonucleotides are synthesized and assembled upon photo-programmable microfluidic chips.

Single or multiple amino acid substitutions, deletions, and/or insertions may be made and tested using known methods of mutagenesis, recombination, and/or shuffling, followed by a relevant screening procedure, such as those disclosed by Reidhaar-Olson and Sauer, 1988, Science 241: 53-57; Bowie and Sauer, 1989, Proc. Natl. Acad. Sci. USA 86: 2152-2156; WO 95/17413; or WO 95/22625. Other methods that may be used include error-prone PCR, phage display (e.g., Lowman et al., 1991, Biochemistry 30: 10832-10837; U.S. Pat. No. 5,223,409; WO 92/06204) and region-directed mutagenesis (Derbyshire et al., 1986, Gene 46: 145; Ner et al., 1988, DNA 7: 127).

Mutagenesis/shuffling methods may be combined with high-throughput, automated screening methods to detect activity of cloned, mutagenized polypeptides expressed by host cells (Ness et al., 1999, Nature Biotechnology 17: 893-896). Mutagenized DNA molecules that encode active polypeptides may be recovered from the host cells and rapidly sequenced using standard methods in the art. These methods allow the rapid determination of the importance of individual amino acid residues in a polypeptide.

Semi-synthetic gene construction is accomplished by combining aspects of synthetic gene construction, and/or site-directed mutagenesis, and/or random mutagenesis, and/or shuffling. Semi-synthetic construction is typified by a process utilizing polynucleotide fragments that are synthesized, in combination with PCR techniques. Defined regions of genes may thus be synthesized de novo, while other regions may be amplified using site-specific mutagenic primers, while yet other regions may be subjected to error-prone PCR or non-error prone PCR amplification. Polynucleotide subsequences may then be shuffled.

The present invention also relates to a method of improving wash performance of a parent polypeptide having the amino acid sequence of SEQ ID NO: 13 or 14, or having at least 80% sequence identity thereto, said method comprising the steps of:

a) a substitution and/or deletion of two, three or four positions in the parent alpha-amylase said positions corresponding to positions 181, G182, D183, and G184 of the mature polypetide of SEQ ID NO: 4; and

b) an alteration at one or more positions corresponding to positions H1, T5, G7, Q11, N16, V17, Q32, A37, T40, P45, W48, G50, T51, N54, V56, K72, R87, Q98, M105, G109, F113, R116, Q118, Q125, G133, T134, W140, G142, G149, T165, W167, Q169, R171, Q172, L173, A174, G184, A186, E190, T193, N195, A204, V206, P211, 1214, V215, L217, L219, A225, L235, V238, K242, S244, M246, M248, L250, G255, Q256, N260, A263, V264, Y267, K269, N270, G273, S280, W284, T285, M286, F289, V291, Y295, Q299, R320, H321, S323, H324, V326, F328, T334, D337, Q345, G346, G348, T355, S376, D377, D379, Y382, S383, Q385, K391, K393, Q395, A420, G423, T444, A445, Q449, T459, P473, C474, G476, G477, and K484 of SEQ ID NO: 13; or H1, A37, T40, N54, V56, A60, K72, G109, F113, R116, N125, F133, T134, T165, Q169, Q172, L173, A174, G184, A186, E190, N195, G196, A204, V206, A225, L228, K242, S244, G255, N260, A265, K269, S280, W284, Y295, S304, R320, H321, S323, V326, K391, I405, A421, A422, A428, R439, G448, W467, D476, and G477 of SEQ ID NO: 14,

wherein the variant has at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 98%, at least 99%, but less than 100% sequence identity with the polypeptide of SEQ ID NO: 4 or 7, and wherein the variant has alpha-amylase activity and improved wash performance compared to the parent polypeptide.

In one embodiment, the variant has at least 50%, such as at least 60%, or at least 70%, or at least 80%, or at least 90%, or at least 100% of the activity of the parent polypeptide having the amino acid sequence of SEQ ID NO: 7.

In one embodiment, the activity is determined according to a Phadebas assay.

The alpha-amylase activity may be determined by a method using the Phadebas substrate (from for example Magle Life Sciences, Lund, Sweden). A Phadebas tablet includes interlinked starch polymers that are in the form of globular microspheres that are insoluble in water. A blue dye is covalently bound to these microspheres. The interlinked starch polymers in the microsphere are degraded at a speed that is proportional to the alpha-amylase activity. When the alpha-amylase degrades the starch polymers, the released blue dye is water soluble and concentration of dye can be determined by measuring absorbance at 620 nm. The concentration of blue is proportional to the alpha-amylase activity in the sample.

The variant sample to be analyzed is diluted in activity buffer with the desired pH. Two substrate tablets are suspended in 5 mL activity buffer and mixed on magnetic stirrer. During mixing of substrate transfer 150 μl to microtiter plate (MTP) or PCR-MTP. Add 30 μl diluted amylase sample to 150 μl substrate and mix. Incubate for 15 minutes at 37° C. The reaction is stopped by adding 30 μl M NaOH and mix. Centrifuge MTP for 5 minutes at 4000×g. Transfer 100 μl to new MTP and measure absorbance at 620 nm.

The alpha-amylase sample should be diluted so that the absorbance at 620 nm is between 0 and 2.2, and is within the linear range of the activity assay.

Thus, in one embodiment, the activity is determined by by a method comprising the steps of;

- a) incubating an alpha-amylase variant according to the invention with a dyed amylose substrate for 15 minute at 37° C.; and
- b) measuring the absorption at OD 620 nm.

In a further embodiment, the activity is determined by a method comprising the steps of;

- a) incubating an alpha-amylase variant according to the invention with a dyed amylose substrate for 15 minute at 37° C.; and
- b) centrifuging the sample;
- c) transferring the supernatant to reader plate, and measuring the absorption at OD 620 nm.

Fermentation Broth Formulations or Cell Compositions

The present invention also relates to a fermentation broth formulation or a cell composition comprising a polypeptide of the present invention. Thus, in one embodiment, the fermentation broth formulation or the cell composition comprises a polynucleotide encoding a variant comprising an alteration in one or more positions corresponding to the positions H1, T5, G7, Q11, N16, V17, Q32, A37, T40, P45, W48, G50, T51, N54, V56, K72, R87, Q98, M105, G109, F113, R116, Q118, Q125, G133, T134, W140, G142, G149, T165, W167, Q169, R171, Q172, L173, A174, G184, A186, E190, T193, N195, A204, V206, P211, 1214, V215, L217, L219, A225, L235, V238, K242, S244, M246, M248, L250, G255, Q256, N260, A263, V264, Y267, K269, N270, G273, S280, W284, T285, M286, F289, V291, Y295, Q299, R320, H321, S323, H324, V326, F328, T334, D337, Q345, G346, G348, T355, S376, D377, D379, Y382, S383, Q385, K391, K393, Q395, A420, G423, T444, A445, Q449, T459, P473, C474, G476, G477, and K484 of SEQ ID NO: 13; or H1, A37, T40, N54, V56, A60, K72, G109, F113, R116, N125, F133, T134, T165, Q169, Q172, L173, A174, G184, A186, E190, N195, G196, A204, V206, A225, L228, K242, S244, G255, N260, A265, K269, S280, W284, Y295, S304, R320, H321, S323, V326, K391, 1405, A421, A422, A428, R439, G448, W467, D476, and G477 of SEQ ID NO: 14, a nucleic acid construct encoding a variant comprising an alteration in one or more positions corresponding to the positions H1, T5, G7, Q11, N16, V17, Q32, A37, T40, P45, W48, G50, T51, N54, V56, K72, R87, Q98, M105, G109, F113, R116, Q118, Q125, G133, T134, W140, G142, G149, T165, W167, Q169, R171, Q172, L173, A174, G184, A186, E190, T193, N195, A204, V206, P211, I214, V215, L217, L219, A225, L235, V238, K242, S244, M246, M248, L250, G255, Q256, N260, A263, V264, Y267, K269, N270, G273, S280, W284, T285, M286, F289, V291, Y295, Q299, R320, H321, S323, H324, V326, F328, T334, D337, Q345, G346, G348, T355, S376, D377, D379, Y382, S383, Q385, K391, K393, Q395, A420, G423, T444, A445, Q449, T459, P473, C474, G476, G477, and K484 of SEQ ID NO: 13; or H1, A37, T40, N54, V56, A60, K72, G109, F113, R116, N125, F133, T134, T165, Q169, Q172, L173, A174, G184, A186, E190, N195, G196, A204, V206, A225, L228, K242, S244, G255, N260, A265, K269, S280, W284, Y295, S304, R320, H321, S323, V326, K391, 1405, A421, A422, A428, R439, G448, W467, D476, and G477 of SEQ ID NO: 14, or an expression vector encoding a variant comprising an alteration in one or more positions corresponding to the positions H1, T5, G7, Q11, N16, V17, Q32, A37, T40, P45, W48, G50, T51, N54, V56, K72, R87, Q98, M105, G109, F113, R116, Q118, Q125, G133, T134, W140, G142, G149, T165, W167, Q269, R171, Q172, L173, A174, G184, A186, E190, T193, N195, A204, V206, P211, I214, V215, L217, L219, A225, L235, V238, K242, S244, M246, M248, L250, G255, Q256, N260, A263, V264, Y267, K269, N270, G273, S280, W284, T285, M286, F289, V291, Y295, Q299, R320, H321, S323, H324, V326, F328, T334, D337, Q345, G346, G348, T355, S376, D377, D379, Y382, S383, Q385, K391, K393, Q395, A420, G423, T444, A445, Q449, T459, P473, C474, G476, G477, and K484 of SEQ ID NO: 13; or H1, A37, T40, N54, V56, A60, K72, G109, F113, R116, N125, F133, T134, T165, Q169, Q172, L173, A174, G184, A186, E190, N195, G196, A204, V206, A225, L228, K242, S244, G255, N260, A265, K269, S280, W284, Y295, S304, R320, H321, S323, V326, K391, I405, A421, A422, A428, R439, G448, W467, D476, and G477 of SEQ ID NO: 14. The fermentation broth product may further comprise additional ingredients used in the fermentation process, such as, for example, cells (including, the host cells containing the gene encoding the polypeptide of the present invention which are used to produce the polypeptide of interest), cell debris, biomass, fermentation media and/or fermentation products. In some embodiments, the composition is a cell-killed whole broth containing organic acid(s), killed cells and/or cell debris, and culture medium.

The term “fermentation broth” as used herein refers to a preparation produced by cellular fermentation that undergoes no or minimal recovery and/or purification. For example, fermentation broths are produced when microbial cultures are grown to saturation, incubated under carbon-limiting conditions to allow protein synthesis (e.g., expression of enzymes by host cells) and secretion into cell culture medium. The fermentation broth can contain unfractionated or fractionated contents of the fermentation materials derived at the end of the fermentation. Typically, the fermentation broth is unfractionated and comprises the spent culture medium and cell debris present after the microbial cells (e.g., filamentous fungal cells) are removed, e.g., by centrifugation. In some embodiments, the fermentation broth contains spent cell culture medium, extracellular enzymes, and viable and/or nonviable microbial cells.

In one embodiment, the fermentation broth formulation and cell compositions comprise a first organic acid component comprising at least one 1-5 carbon organic acid and/or a salt thereof and a second organic acid component comprising at least one 6 or more carbon organic acid and/or a salt thereof. In a particular embodiment, the first organic acid component is acetic acid, formic acid, propionic acid, a salt thereof, or a mixture of two or more of the foregoing and the second organic acid component is benzoic acid, cyclohexanecarboxylic acid, 4-methylvaleric acid, phenylacetic acid, a salt thereof, or a mixture of two or more of the foregoing.

In one embodiment, the composition contains an organic acid(s), and optionally further contains killed cells and/or cell debris. In one embodiment, the killed cells and/or cell debris are removed from a cell-killed whole broth to provide a composition that is free of these components.

The fermentation broth formulations or cell compositions may further comprise a preservative and/or anti-microbial (e.g., bacteriostatic) agent, including, but not limited to, sorbitol, sodium chloride, potassium sorbate, and others known in the art.

The cell-killed whole broth or composition may comprise the unfractionated contents of the fermentation materials derived at the end of the fermentation. Typically, the cell-killed whole broth or composition comprises the spent culture medium and cell debris present after the microbial cells (e.g., filamentous fungal cells) are grown to saturation, incubated under carbon-limiting conditions to allow protein synthesis. In some embodiments, the cell-killed whole broth or composition comprises the spent cell culture medium, extracellular enzymes, and killed filamentous fungal cells. In some embodiments, the microbial cells present in the cell-killed whole broth or composition may be permeabilized and/or lysed using methods known in the art.

A whole broth or cell composition as described herein is typically a liquid, but may comprise insoluble components, such as killed cells, cell debris, culture media components, and/or insoluble enzyme(s). In some embodiments, insoluble components may be removed to provide a clarified liquid composition.

The whole broth formulations and cell compositions of the present invention may be produced by a method described in WO 90/15861 or WO 2010/096673.

Enzyme Compositions

The present invention also relates to compositions comprising a variant according to the invention. Thus, the invention relates to a composition comprising a variant comprising an alteration in one or more positions corresponding to the positions H1, T5, G7, Q11, N16, V17, Q32, A37, T40, P45, W48, G50, T51, N54, V56, K72, R87, Q98, M105, G109, F113, R116, Q118, Q125, G133, T134, W140, G142, G149, T165, W167, Q169, R171, Q172, L173, A174, G184, A186, E190, T193, N195, A204, V206, P211, I214, V215, L217, L219, A225, L235, V238, K242, S244, M246, M248, L250, G255, Q256, N260, A263, V264, Y267, K269, N270, G273, S280, W284, T285, M286, F289, V291, Y295, Q299, R320, H321, S323, H324, V326, F328, T334, D337, Q345, G346, G348, T355, S376, D377, D379, Y382, S383, Q385, K391, K393, Q395, A420, G423, T444, A445, Q449, T459, P473, C474, G476, G477, and K484 of SEQ ID NO: 13; or H1, A37, T40, N54, V56, A60, K72, G109, F113, R116, N125, F133, T134, T165, Q169, Q172, L173, A174, G184, A186, E190, N195, G196, A204, V206, A225, L228, K242, S244, G255, N260, A265, K269, S280, W284, Y295, S304, R320, H321, S323, V326, K391, I405, A421, A422, A428, R439, G448, W467, D476, and G477 of SEQ ID NO: 14.

In one embodiment, the composition comprises a variant comprising

a) a deletion and/or a substitution at two or more positions corresponding to positions R181, G182, D183, and G184 of the amino acid sequence as set forth in SEQ ID NOs: 7 or 10, and

b) an alteration at one or more positions corresponding to positions H1, T5, G7, Q11, N16, V17, Q32, A37, T40, P45, W48, G50, T51, N54, V56, K72,R87, Q98, M105, G109, F113, R116, Q118, Q125, G133, T134, W140, G142, G149, T165, W167, Q169, R171, Q172, T173, A174, A186, E190, T193, N195, A204, V206, P211, V213, 1214, L217, A225, L235, V238, K242, S244, M246, L250, G255, Q256, N260, A263, V264, Y267, K269, N270, G273, S280, W284, T285, M286, F289, V291, Y295, Q299, R320, H321, S323, H324, V326, F328, T334, G337, Q345, G346, T355, S376, D377, S381, Y382, Q385, K391, K393, Q395, A420, G423, T444, A445, Q449, T459, P473, C474, G476, G477, and K484 of SEQ ID NO: 13; or H1, A37, T40, N54, V56, A60, K72, G109, F113, R116, N125, F133, T134, T165, Q169, Q172, L173, A174, G184, A186, E190, N195, G196, A204, V206, A225, L228, K242, S244, G255, N260, A265, K269, S280, W284, Y295, S304, R320, H321, S323, V326, K391, 1405, A421, A422, A428, R439, G448, W467, D476, and G477 of SEQ ID NO: 14. Preferably, the compositions are enriched in such a variant. The term “enriched” as used herein, refers to that the alpha-amylase activity of the composition has been increased, e.g., with an enrichment factor of at least 1.1.

The compositions may comprise a variant of the present invention as the major enzymatic component, e.g., a mono-component composition. In another embodiment, the composition further comprises at least one further active component.

The term “active component” as used herein, refers to any biological or non-biological molecule which in itself is active. For example, an active component is an enzyme.

Thus, in one embodiment, the further active component is an enzyme, such as a protease, lipase, cellulose, pectate lyase and mannanase. Thus, the compositions may comprise multiple enzymatic activities, such as one or more (e.g., several) enzymes selected from the group consisting of hydrolase, isomerase, ligase, lyase, oxidoreductase, or transferase, e.g., an alpha-galactosidase, alpha-glucosidase, aminopeptidase, amylase, beta-galactosidase, beta-glucosidase, beta-xylosidase, carbohydrase, carboxypeptidase, catalase, cellobiohydrolase, cellulase, chitinase, cutinase, cyclodextrin glycosyltransferase, deoxyribonuclease, endoglucanase, esterase, glucoamylase, invertase, laccase, lipase, mannosidase, mutanase, oxidase, pectinolytic enzyme, peroxidase, phytase, polyphenoloxidase, proteolytic enzyme, ribonuclease, transglutaminase, or xylanase.

In one embodiment, the composition is a liquid laundry or liquid dish wash composition, such as an Automatic Dish Wash (ADW) liquid detergent composition, or a powder laundry, such as a soap bar, or powder dish wash composition, such as an ADW unit dose detergent composition and such as a Hand Dish Wash (HDW) detergent composition.

The choice of additional components is within the skills of the skilled person in the art and includes conventional ingredients, including the exemplary non-limiting components set forth below. The choice of components may include, for fabric care, the consideration of the type of fabric to be cleaned, the type and/or degree of soiling, the temperature at which cleaning is to take place, and the formulation of the detergent product. Although components mentioned below are categorized by general header according to a particular functionality, this is not to be construed as a limitation, as a component may comprise additional functionalities as will be appreciated by the skilled artisan.

In one embodiment of the present invention, the variant of the present invention may be added to a detergent composition in an amount corresponding to 0.001-100 mg of protein, such as 0.01-100 mg of protein, preferably 0.005-50 mg of protein, more preferably 0.01-25 mg of protein, even more preferably 0.05-10 mg of protein, most preferably 0.05-5 mg of protein, and even most preferably 0.01-1 mg of protein per liter of wash liquor. The term “protein” in this context is contemplated to be understood to include a variant according to the present invention.

A composition for use in automatic dish wash (ADW), for example, may include 0.0001%-50%, such as 0.001%-20%, such as 0.01%-10%, such as 0.05-5% of enzyme protein by weight of the composition.

A composition for use in laundry granulation, for example, may include 0.0001%-50%, such as 0.001%-20%, such as 0.01%-10%, such as 0.05%-5% of enzyme protein by weight of the composition.

A composition for use in laundry liquid, for example, may include 0.0001%-10%, such as 0.001-7%, such as 0.1%-5% of enzyme protein by weight of the composition.

The variants of the invention as well as the further active components, such as additional enzymes, may be stabilized using conventional stabilizing agents, e.g., a polyol such as propylene glycol or glycerol, a sugar or sugar alcohol, lactic acid, boric acid, or a boric acid derivative, e.g., an aromatic borate ester, or a phenyl boronic acid derivative such as 4-formylphenyl boronic acid, and the composition may be formulated as described in, for example, WO092/19709 and WO92/19708.

In certain markets different wash conditions and, as such, different types of detergents are used. This is disclosed in e.g. EP 1 025 240. For example, In Asia (Japan) a low detergent concentration system is used, while the United States uses a medium detergent concentration system, and Europe uses a high detergent concentration system.

A low detergent concentration system includes detergents where less than about 800 ppm of detergent components are present in the wash water. Japanese detergents are typically considered low detergent concentration system as they have approximately 667 ppm of detergent components present in the wash water.

A medium detergent concentration includes detergents where between about 800 ppm and about 2000 ppm of detergent components are present in the wash water. North American detergents are generally considered to be medium detergent concentration systems as they have approximately 975 ppm of detergent components present in the wash water.

A high detergent concentration system includes detergents where greater than about 2000 ppm of detergent components are present in the wash water. European detergents are generally considered to be high detergent concentration systems as they have approximately 4500-5000 ppm of detergent components in the wash water.

Latin American detergents are generally high suds phosphate builder detergents and the range of detergents used in Latin America can fall in both the medium and high detergent concentrations as they range from 1500 ppm to 6000 ppm of detergent components in the wash water. Such detergent compositions are all embodiments of the invention.

A variant of the present invention may also be incorporated in the detergent formulations disclosed in WO97/07202, which is hereby incorporated by reference.

Examples are given herein of preferred uses of the compositions of the present invention. The dosage of the composition and other conditions under which the composition is used may be determined on the basis of methods known in the art.

In particular, a composition according to the present invention further comprises a chelator.

The term “chelator” as used herein, refers to chemicals which form molecules with certain metal ions, inactivating the ions so that they cannot react with other elements. Thus, a chelator may be defined as a binding agent that suppresses chemical activity by forming chelates. Chelation is the formation or presence of two or more separate bindings between a ligand and a single central atom. The ligand may be any organic compound, a silicate or a phosphate. In the present context the term “chelating agents” comprises chelants, chelating agent, chelating agents, complexing agents, or sequestering agents that forms water-soluble complexes with metal ions such as calcium and magnesium. The chelate effect describes the enhanced affinity of chelating ligands for a metal ion compared to the affinity of a collection of similar nonchelating ligands for the same metal. Chelating agents having binding capacity with metal ions, in particular calcium (Ca2+) ions, and has been used widely in detergents and compositions in general for wash, such as laundry or dish wash. Chelating agents have however shown themselves to inhibit enzymatic activity. The term chelating agent is used in the present application interchangeably with “complexing agent” or “chelating agent” or “chelant”.

Since most alpha-amylases are calcium sensitive the presence of chelating agents these may impair the enzyme activity. The calcium sensitivity of alpha-amylases can be determined by incubating a given alpha-amylase in the presence of a strong chelating agent and analyze the impact of this incubation on the activity of the alpha-amylase in question. A calcium sensitive alpha-amylase will lose a major part or all of its activity during the incubation. Chelating agent may be present in the composition in an amount from 0.0001 wt % to 20 wt %, preferably from 0.01 to 10 wt %, more preferably from 0.1 to 5 wt %.

Non-limiting examples of chelating agents are; EDTA, DTMPA, HEDP, and citrate. Thus, in one embodiment, the composition comprises a variant according to the invention and a chelating agent, such as EDTA, DTMPA, HEDP or citrate.

The term “EDTA” as used herein, refers to ethylene-diamine-tetra-acetic acid which falls under the definition of “strong chelating agents”.

The term “DTMPA” as used herein, refers to diethylenetriamine penta(methylene phosphonic acid). DTMPA can inhibit the scale formation of carbonate, sulfate and phosphate.

The term “HEDP” as used herein, refers to hydroxy-ethane diphosphonic acid, which falls under the definition of “strong chelating agents”.

The chelate effect or the chelating effect describes the enhanced affinity of chelating ligands for a metal ion compared to the affinity of a collection of similar nonchelating ligands for the same metal. However, the strength of this chelate effect can be determined by various types of assays or measure methods thereby differentiating or ranking the chelating agents according to their chelating effect (or strength).

In an assay the chelating agents may be characterized by their ability to reduce the concentration of free calcium ions (Ca2+) from 2.0 mM to 0.10 mM or less at pH 8.0, e.g. by using a test based on the method described by M. K. Nagarajan et al., JAOCS, Vol. 61, no. 9 (September 1984), pp. 1475-1478.

For reference, a chelator having the same ability to reduce the concentration of free calcium ions (Ca2+) from 2.0 mM to 0.10 mM at pH as EDTA at equal concentrations of the chelator are said to be strong chelators.

The composition of the present invention may be in any convenient form, e.g., a bar, a homogenous tablet, a tablet having two or more layers, a pouch having one or more compartments, a regular or compact powder, a granule, a paste, a gel, or a regular, compact or concentrated liquid. There are a number of detergent formulation forms such as layers (same or different phases), pouches, as well as forms for machine dosing unit.

Pouches can be configured as single or multicompartments. It can be of any form, shape and material which is suitable for hold the composition, e.g. without allowing the release of the composition from the pouch prior to water contact. The pouch is made from water soluble film which encloses an inner volume. Said inner volume can be divided into compartments of the pouch. Preferred films are polymeric materials preferably polymers which are formed into a film or sheet. Preferred polymers, copolymers or derivatives thereof are selected polyacrylates, and water soluble acrylate copolymers, methyl cellulose, carboxy methyl cellulose, sodium dextrin, ethyl cellulose, hydroxyethyl cellulose, hydroxypropyl methyl cellulose, malto dextrin, poly methacrylates, most preferably polyvinyl alcohol copolymers and, hydroxypropyl methyl cellulose (HPMC). Preferably the level of polymer in the film for example PVA is at least about 60%. Preferred average molecular weight will typically be about 20,000 to about 150,000. Films can also be of blend compositions comprising hydrolytically degradable and water soluble polymer blends such as polyactide and polyvinyl alcohol (known under the Trade reference M8630 as sold by Chris Craft In. Prod. Of Gary, Ind., US) plus plasticisers like glycerol, ethylene glycerol, Propylene glycol, sorbitol and mixtures thereof. The pouches can comprise a solid laundry cleaning composition or part components and/or a liquid cleaning composition or part components separated by the water soluble film. The compartment for liquid components can be different in composition than compartments containing solids. Ref: (US2009/0011970 A1).

Detergent ingredients may be separated physically from each other by compartments in water dissolvable pouches or in different layers of tablets. Thereby negative storage interaction between components may be avoided. Different dissolution profiles of each of the compartments can also give rise to delayed dissolution of selected components in the wash solution.

A liquid or gel detergent, which is not unit dosed, may be aqueous, typically containing at least 20% by weight and up to 95% water, such as up to about 70% water, up to about 65% water, up to about 55% water, up to about 45% water, up to about 35% water. Other types of liquids, including without limitation, alkanols, amines, diols, ethers and polyols may be included in an aqueous liquid or gel. An aqueous liquid or gel detergent may contain from 0-30% organic solvent. A liquid or gel detergent may be non-aqueous.

Another form of composition is in the form of a soap bar, such as a laundry soap bar, and may be used for hand washing laundry, fabrics and/or textiles. The term “soap bar” as used herein, refers to includes laundry bars, soap bars, combo bars, syndet bars and detergent bars. The types of bar usually differ in the type of surfactant they contain, and the term laundry soap bar includes those containing soaps from fatty acids and/or synthetic soaps. The laundry soap bar has a physical form which is solid and not a liquid, gel or a powder at room temperature. The term “solid” as used herein, refers to a physical form which does not significantly change over time, i.e. if a solid object (e.g. laundry soap bar) is placed inside a container, the solid object does not change to fill the container it is placed in. The bar is a solid typically in bar form but can be in other solid shapes such as round or oval.

The soap bar may also comprise complexing agents like EDTA and HEDP, perfumes and/or different type of fillers, surfactants e.g. anionic synthetic surfactants, builders, polymeric soil release agents, detergent chelators, stabilizing agents, fillers, dyes, colorants, dye transfer inhibitors, alkoxylated polycarbonates, suds suppressers, structurants, binders, leaching agents, bleaching activators, clay soil removal agents, anti-redeposition agents, polymeric dispersing agents, brighteners, fabric softeners, perfumes and/or other compounds known in the art.

The soap bar may be processed in conventional laundry soap bar making equipment such as but not limited to: mixers, plodders, e.g. a two stage vacuum plodder, extruders, cutters, logo-stampers, cooling tunnels and wrappers. The invention is not limited to preparing the soap bars by any single method. The premix of the invention may be added to the soap at different stages of the process. For example, the premix comprising a soap, an enzyme, optionally one or more additional enzymes, a protease inhibitor, and a salt of a monovalent cation and an organic anion may be prepared and the mixture may then plodded. The enzyme and optional additional enzymes may be added at the same time as an enzyme inhibitor, e.g. a protease inhibitor, for example in liquid form. Besides the mixing step and the plodding step, the process may further comprise the steps of milling, extruding, cutting, stamping, cooling and/or wrapping.

Uses

The present invention further relates to the use of a variant according to the present invention in a cleaning process such as laundry or hard surface cleaning including automated dish wash and industrial cleaning. The soils and stains that are important for cleaning are composed of many different substances, and a range of different enzymes, all with different substrate specificities, have been developed for use in detergents both in relation to laundry and hard surface cleaning, such as dishwashing. These enzymes are considered to provide an enzyme detergency benefit, since they specifically improve stain removal in the cleaning process that they are used in, compared to the same process without enzymes. Stain removing enzymes that are known in the art include enzymes such as proteases, amylases, lipases, cutinases, cellulases, endoglucanases, xyloglucanases, pectinases, pectin lyases, xanthanases, peroxidaes, haloperoxygenases, catalases and mannanases.

In one embodiment, the invention relates the use of variants of the present invention in detergent compositions, for use in cleaning hard-surfaces, such as dish wash, or in laundering or for stain removal. In another embodiment, the invention relates to the use of an alpha-amylase variant according to the invention in a cleaning process such as laundry or hard surface cleaning including, but not limited to, dish wash and industrial cleaning. Thus, in one embodiment, the invention relates to the use of a variant comprising an alteration in one or more positions corresponding to the positions H1, T5, G7, Q11, N16, V17, Q32, A37, T40, P45, W48, G50, T51, N54, V56, K72, R87, Q98, M105, G109, F113, R116, Q118, Q125, G133, T134, W140, G142, G149, T165, W167, Q169, R171, Q172, L173, A174, G184, A186, E190, T193, N195, A204, V206, P211, I214, V215, L217, L219, A225, L235, V238, K242, S244, M246, M248, L250, G255, Q256, N260, A263, V264, Y267, K269, N270, G273, S280, W284, T285, M286, F289, V291, Y295, Q299, R320, H321, S323, H324, V326, F328, T334, D337, Q345, G346, G348, T355, S376, D377, D379, Y382, S383, Q385, K391, K393, Q395, A420, G423, T444, A445, Q449, T459, P473, C474, G476, G477, and K484 of SEQ ID NO: 13; or H1, A37, T40, N54, V56, A60, K72, G109, F113, R116, N125, F133, T134, T165, Q169, Q172, L173, A174, G184, A186, E190, N195, G196, A204, V206, A225, L228, K242, S244, G255, N260, A265, K269, S280, W284, Y295, S304, R320, H321, S323, V326, K391, I405, A421, A422, A428, R439, G448, W467, D476, and G477 of SEQ ID NO: 14 in a cleaning process such as laundry or hard surface cleaning including dish wash and industrial cleaning.

In a particular embodiment, the invention relates to the use of a variant comprising a) a substitution and/or deletion of two, three or four positions in the parent alpha-amylase said positions corresponding to positions 181, G182, D183, and G184 of the mature polypetide of SEQ ID NO: 4; and

b) an alteration at one or more positions corresponding to positions positions H1, T5, G7, Q11, N16, V17, Q32, A37, T40, P45, W48, G50, T51, N54, V56, K72, R87, Q98, M105, G109, F113, R116, Q118, Q125, G133, T134, W140, G142, G149, T165, W167, Q169, R171, Q172, L173, A174, G184, A186, E190, T193, N195, A204, V206, P211, I214, V215, L217, L219, A225, L235, V238, K242, S244, M246, M248, L250, G255, Q256, N260, A263, V264, Y267, K269, N270, G273, S280, W284, T285, M286, F289, V291, Y295, Q299, R320, H321, S323, H324, V326, F328, T334, D337, Q345, G346, G348, T355, S376, D377, D379, Y382, S383, Q385, K391, K393, Q395, A420, G423, T444, A445, Q449, T459, P473, C474, G476, G477, and K484 of SEQ ID NO: 13; or H1, A37, T40, N54, V56, A60, K72, G109, F113, R116, N125, F133, T134, T165, Q169, Q172, L173, A174, G184, A186, E190, N195, G196, A204, V206, A225, L228, K242, S244, G255, N260, A265, K269, S280, W284, Y295, S304, R320, H321, S323, V326, K391, 1405, A421, A422, A428, R439, G448, W467, D476, and G477 of SEQ ID NO: 14, in a cleaning process such as laundry or hard surface cleaning including dish wash and industrial cleaning.

In one embodiment of the invention relates the use of a composition according to the invention comprising a variant of the present invention together with one or more surfactants and optionally one or more detergent components, selected from the list comprising of hydrotropes, builders and co-builders, bleaching systems, polymers, fabric hueing agents and adjunct materials, or any mixture thereof in detergent compositions and in detergent applications.

A further embodiment is the use of the composition according to the invention comprising a variant of the present invention together with one or more surfactants, and one or more additional enzymes selected from the group comprising of proteases, lipases, cutinases, cellulases, endoglucanases, xyloglucanases, pectinases, pectin lyases, xanthanases, peroxidaes, haloperoxygenases, catalases and mannanases, or any mixture thereof in detergent compositions and in detergent applications.

In another aspect, the invention relates to a laundering process which may be for household laundering as well as industrial laundering. Furthermore, the invention relates to a process for the laundering of textiles (e.g. fabrics, garments, cloths etc.) where the process comprises treating the textile with a washing solution containing a detergent composition and an alpha-amylase of the present invention. The laundering can for example be carried out using a household or an industrial washing machine or be carried out by hand using a detergent composition containing a glucoamylase of the invention.

In another aspect, the invention relates to a dish wash process which may be for household dish wash as well as industrial dish wash. The term “dish wash” as used herein, refers to both manual dish wash and automated dish wash. Furthermore, the invention relates to a process for the washing of hard surfaces (e.g. cutlery such as knives, forks, spoons; crockery such as plates, glasses, bowls; and pans) where the process comprises treating the hard surface with a washing solution containing a detergent composition and an alpha-amylase variant of the present invention. The hard surface washing can for example be carried out using a household or an industrial dishwasher or be carried out by hand using a detergent composition containing an alpha-amylase of the invention, optionally together with one or more further enzymes selected from the group comprising of proteases, amylases, lipases, cutinases, cellulases, endoglucanases, xyloglucanases, pectinases, pectin lyases, xanthanases, peroxidaes, haloperoxygenases, catalases, mannanases, or any mixture thereof.

In a further aspect, the invention relates to a method for removing a stain from a surface comprising contacting the surface with a composition comprising an alpha-amylase of the present invention together with one or more surfactants and optionally one or more detergent components selected from the list comprising of hydrotropes, builders and co-builders, bleaching systems, polymers, fabric hueing agents and adjunct materials, or any mixture thereof in detergent compositions and in detergent applications. A further aspect is a method for removing a stain from a surface comprising contacting the surface with a composition comprising an alpha-amylase variant of the present invention together with one or more surfactants, one or more additional enzymes selected from the group comprising of proteases, lipases, cutinases, cellulases, endoglucanases, xyloglucanases, pectinases, pectin lyases, xanthanases, peroxidaes, haloperoxygenases, catalases and mannanases, or any mixture thereof in detergent compositions and in detergent applications.

The present invention is further described by the following examples that should not be construed as limiting the scope of the invention.

EXAMPLES
Example 1a
Construction of the Parent Polypeptide as Set Forth in SEQ ID NO: 13

Construction of a hybrid between the A and B domain from a polypeptide having the amino acid sequence set forth SEQ ID NO: 10 (the first parent polypeptide) and the C domain from a polypeptide having the amino acid sequence set forth SEQ ID NO: 11 (the second parent polypeptide).

Based on 3D structural alignment of the two amylases, amino acid no 1 to amino acid no 397 of the first parent polypeptide are defined as domain A and B, and amino acid 398 to amino acid no. 485 of the second parent polypeptide are defined as the C domain. Synthetic DNA fragments coding for part of the A domain from the first parent polypeptide and the C domain from the second parent polypeptide were designed and purchased from an external vendor. In addition to combining fragments of the two different amylases, the following stabilizing substitutions were designed into the synthetic amylase gene: G182* and D183*.

Example 1b
Construction of the Parent Polypeptide as Set Forth in SEQ ID NO: 14

Based on 3D structural alignment of the two amylases, amino acid no 1 to amino acid no 397 of the first parent polypeptide are defined as domain A and B, and amino acid 398 to amino acid no. 485 of the third parent polypeptide are defined as the C domain. Synthetic DNA fragments coding for part of the A domain from the first parent polypeptide and the C domain from the second parent polypeptide were designed and purchased from an external vendor. In addition to combining fragments of the two different amylases, the following stabilizing substitutions were designed into the synthetic amylase gene: G182* and D183*.

The new gene consisting of a gene fragment encoding the A and B domain of the first parent polypeptide and the C domain of the second parent polypeptide coded by the synthetic gene fragments was constructed by triple SOE (splicing by overlap extension) PCR method. A DNA fragment was amplified from an expression clone of the first amylase. The fragments were finally assembled by triple SOE and the derived PCR fragment was transformed into a suitable B. subtilis host and the gene integrated into the B. subtilis chromosome by homologous recombination into the pectate lyase (pel) locus. The gene coding for chloramphenicol acetyltransferase was used as maker (as described in (Diderichsen et al., 1993, Plasmid 30: 312-315). Chloramphenicol resistant clones were analyzed by DNA sequencing to verify the correct DNA sequence of the construct. The resulting amylase consisting of the A and B domain from the first parent polypeptide and the C domain from the second parent polypeptide, and having the deletions of G182* and D183* is the polypeptide as set forth in SEQ ID NO: 14.

Example 2
Generation of Variants According to the Invention

The variants of the present invention have been generated by site-directed mutagenesis. Genomic DNA prepared from the organism containing amylase gene at the Pel locus was used as template for generating the site-directed mutants.

Mutagenic forward primer and PnMi4490 (CAATCCAAGAGAACCCTGATACGGATG—SEQ ID NO: 16) reverse primer was used to generate a ˜3.8 kb fragment. This fragment was used as a megaprimer along with PnMi4491 (CGGAACGCCTGGCTGACAACACG—SEQ ID NO: 17) forward primer to get 6 kb insertion cassette. To enable integration in the Pel locus by double cross-over upon transformation, along with the amylase and cat genes, the cassette contained upstream and downstream Pel sequences at the ends. Selection was done on LB Agar containing chloramphenicol and the mutation was confirmed by DNA sequencing of amylase gene.

A library of variants were generated where different combinations of alterations in the following positions were made; H1, T5, G7, Q11, N16, V17, Q32, A37, T40, P45, W48, G50, T51, N54, V56, A60, K72, R87, Q98, M105, G109, F113, R116, Q118, Q125, G133, T134, W140, G142, G149, T165, W167, Q169, R171, Q172, L173, A174, G184, A186, E190, T193, N195, G196, A204, V206, P211, V213, I214, L217, A225, L228, L235, V238, K242, S244, M246, L2502, G255, N260, A263, V264, A265, Y267, K269, N270, G273, S280, W284, T285, M286, F289, V291, Y295, Q299, S304, R320, H321, S323, H324, V326, F328, T334, G337, Q345, G346, T355, S376, D377, D381, Y382, Q385, K391, and Q395 of SEQ ID NOs: 7 or 10; A420, G423, T444, A445, Q449, T459, P473, C474, G476, G477, and K484 of SEQ ID NO: 13; and I405, A421, A422, A428, G448, D476, and G477 of SEQ ID NO: 14.

The generated variants are listed in the following examples.

Example 3
Wash Performance of Generated Variants Using Automatic Mechanical Stress Assay (AMSA)

In order to assess the wash performance of the variants of the present invention in a detergent base composition, washing experiments may be performed using Automatic Mechanical Stress Assay (AMSA). With the AMSA test the wash performance of a large quantity of small volume enzyme-detergent solutions can be examined. The AMSA plate has a number of slots for test solutions and a lid firmly squeezing the textile swatch to be washed against all the slot openings. During the washing time, the plate, test solutions, textile and lid were vigorously shaken to bring the test solution in contact with the textile and apply mechanical stress in a regular, periodic oscillating manner. For further description see WO 02/42740, especially the paragraph “Special method embodiments” at page 23-24.

General Wash Performance Description

A test solution comprising water (6° dH), 0.79 g/L detergent, e.g. Model detergent J as described below, and the enzyme of the invention at concentration of 0, 0.05 or 0.2 mg enzyme protein/L, is prepared. Fabrics stained with starch (CS-28 from Center For Test materials BV, P.O. Box 120, 3133 KT, Vlaardingen, The Netherlands) was added and washed for 20 minutes at 15° C. and 30° C., or alternatively 20 minutes at 15° C. and 40° C. as specified below. After thorough rinse under running tap water and drying in the dark, the light intensity values of the stained fabrics were subsequently measured as a measure for wash performance. The test with 0 mg enzyme protein/L was used as a blank and corresponded to the contribution from the detergent. Preferably mechanical action is applied during the wash step, e.g. in the form of shaking, rotating or stirring the wash solution with the fabrics. The AMSA wash performance experiments was conducted under the experimental conditions specified below:

TABLE A

Experimental condition

Detergent
Liquid Model detergent J (see Table B)

Detergent dosage
0.79 g/L

Test solution volume
160 micro L

pH
As is

Wash time
20 minutes

Temperature
15° C. or 30° C.

Water hardness
6° dH

Enzyme concentration
0.2 mg enzyme protein/L and 0.05 mg enzyme

in test
protein/L

Test material
CS-28 (Rice starch cotton)

TABLE B

Model detergent J

Content of compound
% active component

Compound
(% w/w)
(% w/w)

LAS
5.15
5.00

linear alkylbenzene

sulfonates

AS
5.00
4.50

alkylbenzene sulfonates

AEOS
14.18
10.00

alkyl ethoxy sulfate

Coco fatty acid
1.00
1.00

AEO
5.00
5.00

alkyl ethoxylate

MEA
0.30
0.30

monoethanolamine

MPG
3.00
3.00

monopropylene glycol

Ethanol
1.50
1.35

DTPA (as Na5 salt)
0.25
0.10

pentasodium

diethylenetriaminepenta-

acetic acid

Sodium citrate
4.00
4.00

Sodium formate
1.00
1.00

Sodium hydroxide
0.66
0.66

H₂O, ion exchanged
58.95
58.95

Water hardness was adjusted to 6° dH by addition of CaCl₂, MgCl₂, and NaHCO₃(Ca²⁺:Mg²⁺:HCO₃₋=2:1:4.5) to the test system. After washing the textiles were flushed in tap water and dried.

TABLE C

Experimental condition

Detergent
Liquid Model detergent A (see Table D)

Detergent dosage
3.33 g/L

Test solution volume
160 micro L

pH
As is

Wash time
20 minutes

Temperature
15° C. or 40° C.

Water hardness
15° dH

Enzyme concentration
0.2 mg enzyme protein/L, 0.05 mg enzyme

in test
protein/L

Test material
CS-28 (Rice starch cotton)

TABLE D

Model detergent A

Content of compound
% active component

Compound
(% w/w)
(% w/w)

LAS
12.00
11.60

(linear alkylbenzene

sulfonates)

AEOS
17.63
4.90

(alkyl ethoxy sulfate),

SLES

(sodium lauryl ether

sulfate)

Soy fatty acid
2.75
2.48

Coco fatty acid
2.75
2.80

AEO (alkyl ethoxylate)
11.00
11.00

Sodium hydroxide
1.75
1.80

Ethanol/Propan-2-ol
3.00
2.70/0.30

MPG
6.00
6.00

monopropylene glycol

Glycerol
1.71
1.70

TEA (triethanolamine)
3.33
3.30

Sodium formate
1.00
1.00

Sodium citrate
2.00
2.00

DTMPA
0.48
0.20

(diethylenetriaminepenta-

acetic acid)

PCA
0.46
0.18

polycarboxylic acid type

polymer

Phenoxy ethanol
0.50
0.50

H₂O, ion exchanged
33.64
33.64

Water hardness was adjusted to 15° dH by addition of CaCl₂, MgCl₂, and NaHCO₃(Ca²⁺:Mg²⁺:HCO₃₋=4:1:7.5) to the test system. After washing the textiles were flushed in tap water and dried.

The wash performance was measured as the brightness expressed as the intensity of the light reflected from the sample when illuminated with white light. When the sample was stained the intensity of the reflected light was lower, than that of a clean sample. Therefore the intensity of the reflected light can be used to measure wash performance.

Color measurements were made with a professional flatbed scanner (EPSON Expression 10000XL, EPSON) used to capture an image of the washed textile.

To extract a value for the light intensity from the scanned images, 48→24 Bit Color pixel values from the image were converted into values for red, green and blue (RGB). The intensity value (Int) is calculated by adding the RGB values together as vectors and then taking the length of the resulting vector:

Int=√{square root over (r²+g²+b²)}

The term “improved wash performance” of the present experiment is defined as displaying an alteration of the wash performance of a variant of the present invention relative to the wash performance of the polypeptide having an amino acid sequence as set forth in SEQ ID NO: 15, The alteration may e.g. be seen as increased stain removal. Improved wash performance was determined as described above. The wash performance was considered to be improved if the Improvement Factor (IF) is at least 1.0, preferably at least 1.05 in one or more of the conditions listed above; i.e. either in Model detergent A at 15° C. or 40° C., where the variant concentration was 0.05 or 0.2 mg/L or in Model detergent J at 15° C. or 30° C., where the variant concentration was 0.05 or 0.2 mg/L. The wash conditions were as described above in Tables A and C.

The terms “Delta intensity” or “Delta intensity value” as used herein refers to the result of an intensity measurement of a test material, e.g. a swatch CS-28 (Center For Testmaterials BV, P.O. Box 120, 3133 KT Vlaardingen, the Netherlands). The swatch was measured with a portion of the swatch, washed under identical conditions, as background. The delta intensity is the intensity value of the test material washed with amylase subtracting the intensity value of the test material washed without amylase.

The wash performance of the variants according to the invention obtained by AMSA are the following;

G182* + D183* + N195F
1.0
1.3
1.1
1.0
1.5
1.3
1.0
1.0

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.1
1.9
1.2
1.2
2.6
2.0
1.5
1.3

N195F + V206L + K391A + G476K

H1* + N54S + V56T + R87S + G109A + A174S + G182* +
2.8
2.2
1.3
1.1
2.6
2.4
1.9
1.5

D183* + N195F + V206L + K391A + G476K

H1* + T40G + N54S + V56T + G109A + A174S + G182* +
2.2
1.7
1.3
1.1
2.5
2.2
1.9
1.6

D183* + N195F + V206L + K391A + G476K

H1* + T51K + N54S + V56T + G109A + A174S + G182* +
2.7
2.1
1.3
1.1
3.2
2.3
2.0
1.5

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + K72R + G109A + A174S + G182* +
2.6
2.2
1.2
1.0
2.6
2.5
1.7
1.5

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + K72H + G109A + A174S + G182* +
2.5
2.4
1.4
1.1
2.6
2.3
1.7
1.6

D183* + N195F + V206L + K391A + G476K

H1* + A37H + N54S + V56T + G109A + A174S + G182* +
2.8
2.7
1.4
1.2
2.8
2.8
1.9
1.6

D183* + N195F + V206L + K391A + G476K

H1* + A37M + N54S + V56T + G109A + A174S + G182* +
2.0
2.3
1.4
1.2
2.7
2.5
2.1
1.6

D183* + N195F + V206L + K391A + G476K

H1* + A37V + N54S + V56T + G109A + A174S + G182* +
3.0
2.6
1.4
1.2
3.1
2.5
2.2
1.7

D183* + N195F + V206L + K391A + G476K

H1* + A37S + N54S + V56T + G109A + A174S + G182* +
2.9
2.5
1.2
1.2
2.9
2.3
1.5
1.3

D183* + N195F + V206L + K391A + G476K

H1* + A37Y + N54S + V56T + G109A + A174S + G182* +
2.6
2.0
1.3
1.2
2.5
2.1
1.5
1.3

D183* + N195F + V206L + K391A + G476K

H1* + A37R + N54S + V56T + G109A + A174S + G182* +
2.2
1.9
1.3
1.2
2.5
2.1
1.4
1.3

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + F113W + A174S + G182* +
2.8
2.2
1.3
1.2
2.7
2.1
1.5
1.2

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + F113S + A174S + G182* +
2.3
1.9
1.3
1.1
2.4
1.7
1.3
1.2

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + F113N + A174S + G182* +
2.0
1.9
1.2
1.2
2.3
2.1
1.4
1.2

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + F113Y + A174S + G182* +
1.9
2.1
1.2
1.2
3.1
2.3
1.5
1.3

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + F113R + A174S + G182* +
1.0
1.4
1.2
1.2
2.0
1.8
1.4
1.3

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + Q118N + A174S + G182* +
1.9
1.8
1.3
1.2
2.9
2.3
1.5
1. text missing or illegible when filed

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + R116Q + A174S + G182* +
2.6
2.0
1.2
1.2
3.5
2.6
1.7
1. text missing or illegible when filed

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + R116V + A174S + G182* +
2.1
2.0
1.1
1.1
2.7
2.3
1.6
1. text missing or illegible when filed

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + R116K + A174S + G182* +
2.2
2.1
1.2
1.1
2.4
2.2
1.4
1. text missing or illegible when filed

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + R116W + A174S + G182* +
1.8
2.0
0.7
0.9
1.1
1.9
1.0
1. text missing or illegible when filed

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + R116L + A174S + G182* +
1.8
1.8
1.2
1.1
1.9
2.0
1.3
1. text missing or illegible when filed

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + G142T + A174S + G182* +
1.6
1.7
1.2
1.1
1.9
2.0
1.3
1. text missing or illegible when filed

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + Q125P + A174S + G182* +
2.1
2.1
1.2
1.1
2.4
2.0
1.4
1.3

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + Q125K + A174S + G182* +
1.9
1.8
1.3
1.1
2.6
1.9
1.5
1.2

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.3
2.1
1.0
1.0
2.5
2.2
1.4
1. text missing or illegible when filed

N195F + V206L + S381G + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.0
1.9
1.1
1.0
2.6
1.8
1.5
1. text missing or illegible when filed

N195F + V206L + M246T + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.9
1.9
1.1
1.1
2.8
2.1
1.5
1. text missing or illegible when filed

N195F + V206L + V213T + K391A + G476K

H1* + A37L + N54S + V56T + G109A + A174S + G182* +
2.5
2.2
1.2
1.1
2.5
2.2
1.8
1. text missing or illegible when filed

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + Q118K + A174S + G182* +
1.8
2.0
1.2
1.1
1.5
1.6
1.4
1. text missing or illegible when filed

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + R116A + A174S + G182* +
2.7
2.3
1.2
1.1
2.4
2.1
1.6
1. text missing or illegible when filed

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + R116H + A174S + G182* +
2.9
2.4
1.3
1.2
2.4
2.3
1.9
1.5

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + Q125A + A174S + G182* +
2.1
2.0
1.1
1.2
3.1
2.1
1.5
1.2

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + Q172G + A174S + G182* +
2.6
2.1
1.4
1.2
2.7
2.2
2.1
1.5

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + Q172R + A174S + G182* +
2.6
2.2
1.4
1.2
2.7
2.4
2.0
1.5

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.4
1.7
1.3
1.2
2.8
1.8
1.7
1.3

N195F + V206L + D377S + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.7
1.7
1.2
1.1
2.3
1.5
1.7
1.3

N195F + V206L + D377G + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.9
1.5
1.1
1.1
2.6
1.6
1.4
1.2

N195F + V206L + D377R + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.0
1.9
1.2
1.0
2.8
1.9
1.5
1.2

N195F + V206L + D377A + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.6
1.6
1.3
1.1
2.9
1.7
1.5
1.1

N195F + V206L + I214G + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.3
1.6
1.4
1.2
2.8
1.9
1.6
1.4

N195F + V206L + S381A + K391A + G476K

H1* + N54S + V56T + G109A + W167I + A174S + G182* +
1.7
1.9
1.3
1.2
2.8
1.8
1.6
1.3

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + W167G + A174S + G182* +
1.5
1.8
1.2
1.1
2.5
1.8
1.5
1.3

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.7
1.8
1.3
1.2
2.8
2.0
1.6
1.3

N195F + V206L + G346S + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.1
1.9
1.3
1.1
2.9
2.1
1.6
1.3

N195F + V206L + G346P + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.2
1.7
1.3
1.1
2.2
2.1
1.7
1.3

N195F + V206L + I214H + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.3
1.8
1.3
1.1
2.9
2.0
1.5
1.3

N195F + V206L + I214S + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.9
1.7
1.3
1.2
2.4
1.7
1.6
1.3

N195F + V206L + I214T + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.0
1.7
1.2
1.1
1.9
1.7
1.3
1.1

N195F + V206L + K391A + A420Q + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.0
1.8
1.2
1.1
2.6
1.8
1.5
1. text missing or illegible when filed

N195F + V206L + K391A + A420S + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.2
1.9
1.3
1.2
2.3
1.8
1.7
1. text missing or illegible when filed

N195F + V206L + K391A + A420K + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.2
2.0
1.3
1.2
2.4
1.9
1.2
1. text missing or illegible when filed

N195F + V206L + K391A + A420L + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.0
1.9
1.3
1.2
2.9
1.9
1.1
1. text missing or illegible when filed

N195F + V206L + K391A + K391Y + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.9
1.9
1.3
1.2
2.5
1.7
1.4
1. text missing or illegible when filed

N195F + V206L + K391A + K391R + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.2
1.9
1.2
1.2
3.4
2.0
2.1
1. text missing or illegible when filed

N195F + V206L + K391A + P473R + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.5
1.8
1.5
1.1
3.4
1.9
1.9
1. text missing or illegible when filed

N195F + V206L + K391A + P473A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.5
1.9
1.3
1.2
2.8
1.9
1.5
1.3

N195F + V206L + K391A + P473G + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.9
1.7
1.3
1.2
2.5
1.9
1.5
1. text missing or illegible when filed

N195F + V206L + K391A + P473T + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.8
1.6
1.3
1.1
2.3
1.8
1.8
1. text missing or illegible when filed

N195F + V206L + K391A + Q395P + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.7
1.8
1.3
1.2
2.8
2.0
1.4
1. text missing or illegible when filed

N195F + V206L + K391A + T444Q + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.7
1.8
1.3
1.2
2.8
2.0
1.5
1. text missing or illegible when filed

N195F + V206L + K391A + T444S + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.3
1.8
1.3
1.3
3.0
2.3
1.7
1. text missing or illegible when filed

N195F + V206L + K391A + T444D + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.0
1.7
1.5
1.2
2.0
2.1
1.8
1. text missing or illegible when filed

N195F + V206L + K391A + T444Y + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.3
1.7
1.3
1.1
2.5
2.0
1.5
1.3

N195F + V206L + S280W + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.1
1.7
1.2
1.1
2.5
1.8
1.4
1.3

N195F + V206L + S280L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.7
1.6
1.1
1.1
2.4
1.6
1.3
1.2

N195F + V206L + S280T + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.9
1.4
1.2
1.0
2.3
1.8
1.3
1.1

N195F + V206L + S280A + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.9
1.7
1.2
1.2
2.6
2.1
1.5
1.4

N195F + V206L + G255N + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.9
1.8
1.3
1.2
2.3
2.2
1.5
1.3

N195F + V206L + T285L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.6
1.7
1.2
1.2
2.2
2.1
1.6
1.3

N195F + V206L + T285Q + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.8
1.6
1.1
1.1
1.8
1.6
1.2
1.2

N195F + V206L + K391A + Q449T + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.8
1.7
1.3
1.1
2.2
2.0
1.3
1.2

N195F + V206L + K391A + P473K + G476K

H1* + N54S + V56T + K72S + G109A + A174S + G182* +
2.5
2.3
1.2
1.1
3.7
2.2
1.5
1.4

D183* + N195F + V206L + K391A + G476K

H1* + T40K + N54S + V56T + G109A + A174S + G182* +
2.3
2.1
1.2
1.1
2.0
1.7
1.5
1.3

D183* + N195F + V206L + G346T + K391A + G476K

H1* + G50A + N54S + V56T + G109A ++ Q172N +
2.4
2.1
1.2
1.2
2.0
1.9
1.4
1.3

A174S + G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.9
1.7
1.3
1.0
2.4
1.6
1.5
1.3

N195F + V206L + K391A + G476K + K484A

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.8
1.8
1.2
1.0
2.2
1.8
1.4
1.2

N195F + V206L + K391A + G476K + K484G

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.8
1.7
1.1
1.0
2.4
2.0
1.4
1.3

N195F + V206L + K391A + G476K + K484P

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.9
1.4
1.2
1.1
2.5
1.7
1.3
1.2

N195F + V206L + K391A + G476K + K484E

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.7
1.5
1.1
1.0
2.0
1.3
1.3
1.2

N195F + V206L + K391A + G476K + K484Q

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.3
2.0
1.2
1.2
2.7
1.7
1.6
1.4

N195F + V206L + K391A + G476K + K484S

H1*K + G50A + N54S + V56T + G109A + W167F + A174S +
2.1
1.9
1.1
1.1
2.2
1.7
1.3
1. text missing or illegible when filed

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + L173V + A174S + G182* +
2.2
1.8
1.1
1.1
2.2
1.7
1.1
1. text missing or illegible when filed

D183* + N195F + A204T + V206L + K391A + G476K

H1* + N54S + V56T + G109A + W140Y + A174S + G182* +
2.2
1.9
1.2
1.1
2.5
1.9
1.3
1. text missing or illegible when filed

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + Q98S + G109A + A174S + G182* +
1.9
1.8
1.2
1.1
2.1
1.8
1.1
1. text missing or illegible when filed

D183* + N195F + V206L + R320A + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.9
1.7
1.1
1.1
2.1
1.5
1.2
1. text missing or illegible when filed

N195F + V206L + H324L + K391A + G476K

H1* + N54S + V56T + G109A + G149Q + A174S + G182* +
1.7
1.9
1.2
1.2
1.6
1.4
1.0
1. text missing or illegible when filed

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.1
2.0
1.2
1.2
2.3
1.7
1.2
1. text missing or illegible when filed

N195F + V206L + G346T + K391A + G476K + G477A

H1* + N54S + V56T + G109AG255A ++ A174S + G182* +
2.1
2.0
1.2
1.2
2.3
1.8
1.2
1.2

D183* + N195F + V206L + K391A + G476K

H1* + G50A + N54S + V56T + G109A + F113L + R116L +
2.3
2.2
1.2
1.1
2.6
2.2
1.3
1. text missing or illegible when filed

A174S + G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + W167F + A174S + G182* +
2.0
1.9
1.1
1.0
2.3
2.0
1.4
1. text missing or illegible when filed

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + L173V + A174S + G182* +
1.8
1.9
1.0
1.0
2.2
1.8
1.4
1. text missing or illegible when filed

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + K72R + G109A + W167F + A174S +
2.1
1.7
1.1
1.1
2.9
2.0
1.4
1. text missing or illegible when filed

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.8
1.4
1.0
1.1
2.4
1.9
1.4
1. text missing or illegible when filed

N195F + V206L + K391A + G476K + G477Q

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.6
1.5
1.0
1.0
2.2
1.7
1.3
1.2

N195F + V206L + Q345D + K391A + G476K + G477Q

H1* + G50A + N54S + V56T + G109A + Q172D + A174S +
1.9
1.9
0.9
1.1
2.1
1.9
1.6
1.3

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.9
1.9
0.7
1.0
2.2
2.0
1.4
1.3

N195F + V206L + V264I + K391A + G423H + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.1
2.0
0.8
1.0
2.1
2.1
1.5
1.3

N195F + V206L + R320V + K391A + G476Y

H1* + T5K + N54S + V56T + G109A + A174S + G182* +
1.7
1.8
0.9
1.0
1.8
1.7
1.4
1.2

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + R116M + A174S + G182* +
2.9
2.2
1.2
1.1
2.8
2.1
1.2
1.1

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + Q172Y + A174S + G182* +
2.1
1.8
1.1
1.1
1.9
1.8
1.4
1.1

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.0
1.7
1.1
1.1
1.9
1.7
1.3
1.2

N195F + V206L + I214L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.2
1.9
1.2
1.2
2.2
2.2
1.3
1.2

N195F + V206L + L217V + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.2
1.7
1.2
1.2
2.3
2.1
1.4
1.2

N195F + V206L + L217H + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.1
2.0
1.1
1.1
2.3
2.1
1.2
1.2

N195F + V206L + P211D + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.1
1.9
1.1
1.2
2.3
1.9
1.3
1.3

N195F + V206L + K391A + K391Q + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.0
1.9
1.1
1.1
2.3
1.9
1.4
1.2

N195F + V206L + K391A + T444H + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.2
1.7
1.1
1.0
2.3
1.8
1.2
1.2

N195F + V206L + K391A + T444V + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.0
1.6
1.2
1.1
1.9
1.8
1.3
1.2

N195F + V206L + K391A + T444R + G476K

H1* + G50A + N54S + V56T + G109A + L173V + A174S +
2.4
2.1
1.2
1.2
3.2
2.2
1.6
1.4

G182* + D183* + N195F + A204V + V206L + K391A + G476K

H1* + G50A + N54S + V56T + G109A + F113L + W167F +
1.9
1.7
1.0
1.1
2.1
1.8
1.2
1. text missing or illegible when filed

A174S + G182* + D183* + N195F + V206L + K391A + G476K

H1* + G7K + N54S + V56T + G109A + A174S + G182* +
2.0
1.8
1.1
1.1
2.7
2.1
1.6
1. text missing or illegible when filed

D183* + N195F + V206L + R320A + K391A + G476K

H1* + N54S + V56T + G109A + Q118G + A174S + G182* +
1.7
1.8
1.1
1.1
2.4
1.6
1.5
1. text missing or illegible when filed

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.6
1.6
1.0
1.1
1.6
1.6
1.3
1. text missing or illegible when filed

N195F + V206L + S280K + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.4
1.6
1.1
1.1
1.8
1.6
1.4
1. text missing or illegible when filed

N195F + V206L + N270G + K391A + G476K

H1* + N54S + V56T + G109A + Q172D + A174S + G182* +
2.1
1.9
1.2
1.1
3.1
2.3
1.6
1. text missing or illegible when filed

D183* + N195F + V206L + G346T + K391A + G476K + G477A

H1* + N54S + V56T + G109A + Q118S + A174S + G182* +
2.1
1.9
1.1
1.1
1.8
1.5
1.4
1.2

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.0
1.9
1.2
1.2
2.3
1.7
1.5
1. text missing or illegible when filed

N195F + V206L + R320A + K391A + G476K

H1* + N54S + V56T + G109A + T134E + A174S + G182* +
2.4
2.1
1.2
1.1
2.8
2.4
1.4
1. text missing or illegible when filed

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.7
1.8
1.0
1.0
1.8
1.8
1.2
1. text missing or illegible when filed

N195F + V206L + G337H + Q385L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.0
1.8
1.1
1.1
2.2
1.9
1.3
1. text missing or illegible when filed

N195F + V206L + Y382M + K391A + G476K

H1* + N54S + V56T + K72R + G109A + A174S + G182* +
1.9
1.9
1.1
1.1
2.7
2.2
1.4
1. text missing or illegible when filed

D183* + N195F + V206L + G255A + D377H + K391A + G476K

H1* + N54S + V56T + K72R + G109A + T134E + A174S +
2.4
2.0
1.2
1.1
3.0
2.3
1.4
1.3

G182* + D183* + N195F + V206L + G255A + K391A + G476K

H1* + N54S + V56T + K72R + M105F + G109A + A174S +
2.0
1.7
1.2
1.1
2.5
2.0
1.4
1.2

G182* + D183* + N195F + V206L + G255A + K391A + G476K

H1* + N54S + V56T + M105F + G109A + T134E + A174S +
2.0
1.9
1.0
1.0
2.3
2.1
1.2
1.1

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + M105F + G109A + Q118F + T134E +
1.9
1.7
1.1
1.1
1.7
1.9
1.3
1.2

A174S + G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.0
1.9
1.1
1.0
2.3
2.0
1.4
1.3

N195F + V206L + R320A + S323N + D377H + K391A + G476K

H1* + T51E + N54S + V56T + G109A + A174H + G182* +
2.3
1.9
1.2
1.1
2.6
2.2
1.6
1.4

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + L173A + A174S + G182* +
2.4
2.0
1.3
1.2
2.4
2.0
1.6
1.4

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.5
2.0
1.3
1.2
2.4
1.6
1.6
1.4

N195F + V206L + Q299V + K391A + G476K

H1* + N54S + V56T + G109A + L173G + A174S + G182* +
2.2
2.0
1.3
1.2
2.3
1.9
1.7
1.4

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.1
2.0
1.3
1.2
2.4
2.0
1.5
1.3

N195F + V206L + T355L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.3
1.7
1.0
1.0
1.9
1.6
1.2
1.1

N195F + V206L + T355F + K391A + G476K

H1* + G50A + N54S + V56T + G109A + A174S + G182* +
2.3
2.0
1.2
1.1
2.1
1.8
1.4
1.3

D183* + N195F + A204T + V206L + K391A + G476K

H1* + G50A + N54S + V56T + G109A + A174E + G182* +
1.8
1.6
1.1
1.1
2.1
1.8
1.2
1.2

D183* + N195F + A204T + V206L + K391A + G476K

H1* + N54S + V56T + K72R + G109A + A174S + G182* +
2.2
1.8
1.2
1.1
2.7
2.1
1.4
1. text missing or illegible when filed

D183* + N195F + V206L + R320A + S323N + K391A + G476K

H1* + N54S + V56T + M105I + G109A + A174S + G182* +
1.8
1.8
1.1
1.1
1.5
1.5
1.3
1. text missing or illegible when filed

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.0
1.8
1.1
1.0
1.8
1.6
1.3
1. text missing or illegible when filed

N195F + V206L + M246L + K391A + G476K

H1* + T51G + N54S + V56T + G109A + A174N + G182* +
1.6
1.5
1.0
1.0
2.1
1.7
1.3
1. text missing or illegible when filed

D183* + N195F + V206L + K391A + G476K

H1* + T51A + N54S + V56T + G109A + A174G + G182* +
1.4
1.5
0.9
0.9
1.6
1.5
1.3
1. text missing or illegible when filed

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174D + G182* + D183* +
1.9
1.5
1.0
1.0
1.5
1.5
1.1
1. text missing or illegible when filed

N195F + V206L + K391A + G476K

H1* + T51S + N54S + V56T + G109A + A174P + G182* +
1.7
1.7
0.9
1.0
1.1
1.2
0.8
0. text missing or illegible when filed

D183* + N195F + V206L + K391A + G476K

H1* + T51G + N54S + V56T + G109A + A174M + G182* +
1.3
1.5
1.0
1.0
1.6
1.5
0.9
1.1

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + K72A + W167R + A174S +
0.9
1.2
0.5
0.7
0.7
0.8
0.4
0. text missing or illegible when filed

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + K72A + G109A + W167S + A174S +
1.6
1.8
0.4
0.8
0.7
1.2
0.6
0. text missing or illegible when filed

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + K72R + G109A + W167R + A174S +
1.1
1.1
0.8
0.8
0.7
0.8
0.7
0. text missing or illegible when filed

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + K72Q + G109A + W167H + A174S +
2.4
2.2
0.9
1.0
1.8
1.7
1.0
1. text missing or illegible when filed

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + Q118R + T134E + A174S +
1.8
1.7
1.1
1.0
2.1
1.7
1.2
1. text missing or illegible when filed

G182* + D183* + N195F + V206L + D377H + K391A + G476K

H1* + Q11H + N54S + V56T + G109A + Q118R + T134E +
1.9
1.6
1.1
1.0
2.2
1.6
1.3
1.3

A174S + G184T + G182* + D183* + N195F + V206L +

D377H + K391A + G476K

H1* + N54S + V56T + K72R + G109A + T134E + A174S +
2.3
2.1
1.2
1.1
3.2
1.9
1.3
1.3

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + K72R + G109A + T134E + A174S +
2.2
2.0
1.1
1.0
3.8
1.9
1.3
1.2

G182* + D183* + N195F + V206L + D377H + K391A + G476K

H1* + N54S + V56T + K72R + G109A + T134E + A174S +
2.2
2.0
1.2
1.1
3.9
2.4
1.4
1.3

G182* + D183* + G184T + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + Q118R + T134E + A174S +
2.0
1.9
1.0
1.2
2.5
2.0
1.2
1.1

G182* + D183* + N195F + V206L + G255A + K391A + G476K

H1* + N54S + V56T + G109A + Q118R + A174S + G182* +
2.2
1.9
1.1
1.1
2.8
1.8
1.2
1.2

D183* + N195F + V206L + D377H + K391A + G476K

H1* + N54S + V56T + G109A + T134E + A174S + G182* +
2.1
2.1
1.0
1.1
3.4
2.3
1.3
1.3

D183* + G184T + N195F + V206L + D377H + K391A + G476K

H1* + N54S + V56T + G109A + Q172D + A174Q + G182* +
2.0
1.9
1.2
1.1
3.0
2.2
1.1
1.2

D183* + N195F + V206L + K391A + G476K + G477A

H1* + N54S + V56T + K72A + G109A + W167F + A174S +
1.1
1.4
0.6
0.8
1.5
1.3
1.0
0.8

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + W167S + G182* + D183* +
1.4
1.3
0.8
1.0
1.5
1.6
1.3
1.1

N195F + A174S + V206L + K391A + G476K

H1* + N54S + V56T + K72H + G109A + W167S + A174S +
1.4
1.4
0.5
1.0
1.0
1.4
0.8
1.1

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + K72R + G109A + W167T + A174S +
1.3
1.2
0.8
0.8
1.2
1.0
1.1
1.0

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + K72E + G109A + A174S + G182* +
1.3
1.4
0.8
0.9
1.4
1.3
0.9
0.9

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + K72R + G109A + W167L + A174S +
1.6
1.6
1.1
1.1
2.0
1.5
1.4
1. text missing or illegible when filed

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + K72R + G109A + W167M + A174S +
1.5
1.6
0.8
1.0
1.5
1.3
1.2
1. text missing or illegible when filed

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + K72R + G109A + W167H + A174S +
1.3
1.4
0.6
1.0
1.6
1.3
0.9
1. text missing or illegible when filed

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + K72R + G109A + A174S + G182* +
2.0
1.9
1.2
1.1
2.5
2.0
1.4
1. text missing or illegible when filed

D183* + G184T + N195F + V206L + G255A + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.2
1.9
1.1
1.1
2.3
1.9
1.5
1. text missing or illegible when filed

G184T + N195F + V206L + D377H + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.1
2.0
1.2
1.0
2.1
1.7
1.3
1. text missing or illegible when filed

N195F + V206L + G255A + D377H + K391A + G476K

H1* + N54S + V56T + G109A + Q118R + A174S + G182* +
1.2
1.5
1.0
1.0
1.8
1.2
1.0
1. text missing or illegible when filed

D183* + N195F + V206L + G255A + D377H + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.2
1.3
0.9
1.0
1.3
1.3
1.0
1. text missing or illegible when filed

G184A + T193K + N195F + V206L + K391A + G476K

H1* + N54S + V56T + M105F + G109A + A174S + G182* +
1.9
2.0
1.1
1.2
2.1
1.9
1.4
1. text missing or illegible when filed

D183* + G184T + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + T134E + A174S + G182* +
1.7
2.1
1.1
1.1
2.1
2.0
1.3
1. text missing or illegible when filed

D183* + N195F + V206L + R320A + S323N + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.7
1.8
1.2
1.1
2.1
1.9
1.4
1. text missing or illegible when filed

N195F + V206L + V291G + D377H + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.7
1.9
1.1
1.1
2.1
1.9
1.3
1.3

N195F + V206L + V291A + F328L + D377H + K391A + G476K

H1* + V56T + G109A + A174S + G182* + D183* + N195F +
1.3
1.8
1.1
1.1
1.5
1.5
1.3
1.2

V206L + D377H + K391A + G476K

H1* + N54S + V56T + G109A + T134E + A174S + G182* +
2.4
2.2
1.1
1.1
3.0
2.4
1.5
1.3

D183* + G184T + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.7
1.8
1.2
1.1
2.1
1.9
1.4
1.2

N195F + V206L + V238A + D377H + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.0
1.8
1.1
1.1
2.5
1.9
1.3
1.2

N195F + V206L + V238T + D377H + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.9
1.6
1.1
1.1
2.3
1.8
1.2
1.2

N195F + V206L + V238G + D377H + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.6
1.8
1.0
1.0
2.1
1.8
1.2
1.2

N195F + V206L + F328M + D377H + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.6
1.7
0.9
1.0
1.7
1.5
1.0
1.1

N195F + V206L + F328L + D377H + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.6
1.6
0.8
1.0
1.8
1.7
1.0
1.1

N195F + V206L + F328V + D377H + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.6
1.6
0.9
1.1
2.0
1.6
1.1
1.0

N195F + V206L + F328I + D377H + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.9
1.8
1.2
1.1
2.4
1.9
1.3
1.2

N195F + V206L + V291T + D377H + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.4
1.9
1.2
1.2
2.9
2.1
1.5
1.3

N195F + V206L + V291A + D377H + K391A + G476K

H1* + N54S + V56T + K72R + G109A + T134E + A174S +
2.1
1.9
1.0
1.1
2.1
2.0
1.4
1.2

G182* + D183* + G184T + N195F + V206L + G255A +

K391A + G476K

H1* + N54S + V56T + G109A + L173H + A174S + G182* +
2.0
1.8
1.2
1.1
2.6
2.0
1.4
1.2

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + Q125T + A174S + G182* +
1.4
1.7
0.9
1.0
2.0
1.8
1.2
1.0

D183* + N195F + V206L + K391A + G476K

H1* + N16S + N54S + V56T + G109A + A174S + G182* +
1.9
1.6
1.0
1.0
2.1
1.9
1.3
1.2

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + K72N + G109A + A174S + G182* +
2.1
1.6
1.0
1.0
2.1
2.2
1.3
1. text missing or illegible when filed

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + K72N + G109A + W167L + A174S +
2.2
1.9
0.9
1.2
1.9
2.1
1.2
1. text missing or illegible when filed

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + K72A + G109A + W167Y + A174S +
1.7
1.8
0.8
1.1
1.6
2.0
1.1
1. text missing or illegible when filed

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + Q118R + T134E + A174S +
2.0
1.9
1.0
1.1
2.0
2.0
1.2
1. text missing or illegible when filed

G182* + D183* + N195F + V206L + G255A + D377H +

K391A + G476K

H1* + N54S + V56T + K72R + G109A + A174S + G182* +
2.2
2.1
1.1
1.1
3.3
2.5
1.5
1. text missing or illegible when filed

D183* + N195F + G184T + V206L + K391A + G476K

H1* + N54S + V56T + K72R + G109A + Q118R + A174S +
0.9
1.3
0.8
0.9
1.3
1.5
1.0
1. text missing or illegible when filed

G182* + D183* + G184T + N195F + V206L + D377H +

K391A + G476K

H1* + N54S + V56T + K72R + G109A + Q118R + A174S +
2.4
2.1
1.2
1.2
2.8
2.2
1.5
1. text missing or illegible when filed

G182* + D183* + N195F + V206L + G255A + D377H +

K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.9
1.8
1.2
1.1
2.5
2.0
1.5
1. text missing or illegible when filed

G184T + N195F + V206L + R320A + S323N + K391A + G476K

H1* + N54S + G109A + A174S + G182* + D183* + N195F +
1.5
1.7
1.1
1.1
2.3
1.8
1.4
1. text missing or illegible when filed

V206L + D377H + K391A + G476K

H1* + N54S + V56T + A174S + G182* + D183* + N195F +
1.8
1.7
1.1
0.9
2.1
1.7
1.4
1. text missing or illegible when filed

V206L + D377H + K391A + G476K

H1* + N54S + V56T + G109A + G182* + D183* + N195F +
2.0
1.8
1.2
1.2
2.6
1.7
1.3
1.2

V206L + D377H + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.6
1.6
1.3
1.1
2.8
2.1
1.5
1.2

N195F + D377H + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.0
1.6
1.1
1.1
2.6
2.0
1.3
1.1

N195F + D377H + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.2
2.0
1.2
1.1
2.6
1.9
1.5
1.4

N195F + V206L + D377H + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.0
1.9
1.2
1.1
2.5
1.8
1.6
1.4

N195F + V206L + D377H + K391A

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.4
1.6
1.1
1.0
1.5
1.7
1.3
1.0

N195F + V206L + D377R + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.5
1.7
1.1
1.0
2.3
1.2
1.4
1.2

N195F + V206L + L217T + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.0
1.8
1.3
1.2
2.4
1.8
1.5
1.4

N195F + V206L + D377S + K391A + G476K

H1* + N54S + V56T + G109A + R116E + A174S + G182* +
2.0
1.9
1.2
1.1
2.3
2.0
1.5
1.3

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.5
1.7
1.0
1.1
1.7
1.4
1.2
1.2

N195F + V206L + L217Q + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.3
1.5
1.0
1.0
1.7
1.6
1.2
1.2

N195F + V206L + G346D + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.9
1.7
1.1
1.2
2.5
1.7
1.4
1.3

N195F + V206L + D377A + K391A + G476K

H1* + N54S + V56T + G109A + T134E + A174S + G182* +
2.2
2.1
1.2
1.1
2.3
2.1
1.1
1.2

D183* + N195F + V206L + D377H + K391A + G476K

H1* + N54S + V56T + G109A + Q118R + T134E + A174S +
1.9
1.7
1.3
1.2
2.0
1.7
1.2
1.2

G182* + D183* + G184T + N195F + V206L + K391A + G476K

H1* + N54S + V56T + K72R + G109A + Q118R + T134E +
1.9
1.7
1.1
1.1
2.1
1.9
1.0
1.1

A174S + G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.7
1.6
1.2
1.1
2.3
1.9
1.2
1.1

N195F + V206L + G346P + K391A + G476K

H1* + N54S + V56T + G109A + R116S + A174S + G182* +
2.0
1.8
1.2
1.1
2.1
1.9
1.1
1. text missing or illegible when filed

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.7
1.8
1.2
1.1
2.0
2.0
1.2
1. text missing or illegible when filed

N195F + V206L + D377Q + K391A + G476K

H1* + N54S + V56T + G109A + R116I + A174S + G182* +
2.2
1.8
1.2
1.1
2.1
2.0
1.3
1. text missing or illegible when filed

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174F + G182* + D183* +
1.7
1.5
1.1
1.0
1.5
1.6
1.3
1. text missing or illegible when filed

N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + R116G + A174S + G182* +
2.0
1.9
1.2
1.2
2.1
2.0
1.0
1. text missing or illegible when filed

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174T + G182* + D183* +
1.9
1.8
1.2
1.2
1.9
2.0
1.1
1. text missing or illegible when filed

N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + Q118R + T134E + A174S +
1.5
1.8
1.1
1.0
1.9
1.9
1.4
1. text missing or illegible when filed

G182* + D183* + G184T + N195F + V206L + G255A +

K391A + G476K

H1* + N54S + V56T + K72R + G109A + Q118R + T134E +
1.7
1.8
1.1
1.0
2.2
1.8
1.5
1. text missing or illegible when filed

A174S + G182* + D183* + N195F + V206L + G255A +

K391A + G476K

H1* + T51G + N54S + V56T + G109A + Q172R + A174S +
1.2
1.6
1.0
1.0
1.6
1.4
1.3
1. text missing or illegible when filed

G182* + D183* + N195F + V206L + K391A + G476K

H1* + T51A + N54S + V56T + G109A + Q172D + A174S +
1.5
1.7
1.0
1.0
2.3
1.9
1.4
1. text missing or illegible when filed

G182* + D183* + N195F + V206L + K391A + G476K

H1* + T51S + N54S + V56T + G109A + Q172M + A174S +
1.7
1.6
1.0
1.0
2.0
1.8
1.2
1. text missing or illegible when filed

G182* + D183* + N195F + V206L + K391A + G476K

H1* + T51A + N54S + V56T + G109A + Q172S + A174S +
2.4
1.8
1.3
1.1
2.8
2.1
1.7
1. text missing or illegible when filed

G182* + D183* + N195F + V206L + K391A + G476K

H1* + T51E + N54S + V56T + G109A + Q172T + A174S +
2.0
1.8
0.9
1.0
2.6
1.9
1.6
1.3

G182* + D183* + N195F + V206L + K391A + G476K

H1* + T51A + N54S + V56T + G109A + Q172A + A174S +
1.9
1.8
1.0
1.1
2.3
1.9
1.6
1.3

G182* + D183* + N195F + V206L + K391A + G476K

H1* + T51S + N54S + V56T + G109A + Q172N + A174S +
2.2
1.9
1.1
1.1
2.4
1.9
1.5
1.3

G182* + D183* + N195F + V206L + K391A + G476K

H1* + T51G + N54S + V56T + G109A + Q172K + A174S +
1.4
1.3
1.0
0.9
1.7
1.5
1.2
1.1

G182* + D183* + N195F + V206L + K391A + G476K

H1* + T51A + N54S + V56T + G109A + Q172G + A174S +
1.7
1.7
1.2
1.0
2.2
1.9
1.4
1.2

G182* + D183* + N195F + V206L + K391A + G476K

H1* + T51E + N54S + V56T + G109A + Q172L + A174S +
2.0
1.9
1.0
1.1
2.2
1.8
1.2
1.1

G182* + D183* + N195F + V206L + K391A + G476K

H1* + T51E + N54S + V56T + G109A + Q172R + A174S +
2.0
1.9
1.1
1.1
1.8
1.7
1.4
1.1

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + Q172K + A174S + G182* +
2.1
1.8
1.0
1.1
2.0
1.6
1.3
1.2

D183* + N195F + V206L + K391A + G476K

H1* + T51D + N54S + V56T + G109A + Q172N + A174S +
1.8
1.7
1.0
1.0
1.9
1.6
1.3
1.1

G182* + D183* + N195F + V206L + K391A + G476K

H1* + T51A + N54S + V56T + G109A + Q172K + A174S +
1.7
1.7
1.2
1.1
1.8
1.4
1.3
1.2

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + K72R + G109A + Q172S + A174S +
2.0
1.8
1.3
1.1
2.6
1.8
1.4
1.3

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + K72H + G109A + Q172T + A174S +
2.0
1.8
1.2
1.1
2.0
1.9
1.3
1.2

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + K72R + G109A + Q172R + A174S +
1.8
1.6
1.1
1.1
1.4
1.5
1.1
1.1

K179E + G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + K72R + G109A + Q172M + A174S +
1.9
1.8
1.2
1.1
2.1
1.7
1.3
1.2

G182* + D183* + N195F + V206L + Y295N + K391A + G476K

H1* + N54S + V56T + K72R + G109A + Q172R + A174S +
1.4
1.4
1.0
1.0
1.5
1.4
1.2
0.9

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + K72Q + G109A + Q172R + A174S +
2.0
2.0
1.0
1.0
2.6
2.1
1.3
1.2

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + K72R + G109A + Q172T + A174S +
2.1
2.1
1.3
1.1
2.3
2.0
1.4
1. text missing or illegible when filed

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + K72M + G109A + Q172S + A174S +
2.3
1.9
1.2
1.1
2.4
2.0
1.2
1. text missing or illegible when filed

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + K72Q + G109A + Q172M + A174S +
2.1
2.1
1.3
1.1
2.8
2.2
1.5
1. text missing or illegible when filed

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + Q172M + A174S + G182* +
1.9
2.0
1.2
1.1
2.4
1.7
1.4
1. text missing or illegible when filed

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + K72S + G109A + A174H + G182* +
2.3
2.0
1.2
1.1
2.2
2.1
1.4
1. text missing or illegible when filed

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + K72Q + G109A + A174D + G182* +
2.3
2.1
1.1
1.1
2.2
1.9
1.4
1. text missing or illegible when filed

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + K72R + G109A + A174D + G182* +
2.5
2.0
1.1
1.1
2.3
2.1
1.4
1. text missing or illegible when filed

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + K72Q + G109A + A174K + G182* +
2.2
1.9
1.2
1.1
2.6
2.0
1.5
1.3

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174G + G182* + D183* +
2.3
1.9
1.2
1.1
2.6
2.2
1.5
1. text missing or illegible when filed

N195F + V206L + K391A + G476K

H1* + N54S + V56T + K72R + G109A + A174Q + G182* +
2.5
2.1
1.2
1.2
2.3
2.1
1.5
1. text missing or illegible when filed

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + K72Q + G109A + A174G + G182* +
2.3
2.1
1.1
1.1
2.6
2.2
1.4
1. text missing or illegible when filed

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + K72S + G109A + A174N + G182* +
2.6
2.1
1.3
1.1
2.6
2.3
1.5
1. text missing or illegible when filed

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + K72R + G109A + A174H + G182* +
2.1
1.9
1.3
1.2
2.5
2.2
1.5
1. text missing or illegible when filed

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.7
1.7
1.1
1.1
1.9
1.6
1.4
1. text missing or illegible when filed

N195F + V206L + K391A + K391S + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.7
1.6
1.2
1.1
2.2
1.6
1.4
1.2

N195F + V206L + K391A + T444A + G476K

H1* + N54S + V56T + G109A + F113L + W167F + A174S +
1.7
1.6
1.0
1.0
2.1
1.7
1.2
1.2

G182* + D183* + N195F + V206L + K391A + G476K

H1* + G50A + N54S + V56T + G109A + A174E + G182* +
2.0
1.8
1.0
1.1
1.5
1.7
1.3
1.2

D183* + N195F + A204T + V206L + S323N + K391A + G476K

H1* + N54S + V56T + K72R + G109A + Q118R + A174S +
1.6
1.6
1.1
1.0
1.2
1.3
1.2
1.2

G182* + D183* + G184T + N195F + V206L + G255A +

K391A + G476K

H1* + N54S + V56T + M105F + G109A + A174S + G182* +
1.8
1.9
1.2
1.1
2.6
1.9
1.5
1.3

D183* + N195F + V206L + D377H + K391A + G476K

H1* + N54S + V56T + K72R + G109A + Q118R + A174S +
1.5
1.6
1.1
1.1
1.4
1.3
1.3
1.2

G182* + D183* + N195F + V206L + D377H + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.7
2.1
1.2
1.2
2.9
2.6
1.6
1.4

N195F + A204G + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.8
1.8
1.0
0.9
1.8
1.7
1.4
1.2

N195F + V206L + A225F + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.8
1.6
0.9
0.9
1.7
1.8
1.2
1.2

N195F + V206L + M246A + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.1
1.7
0.9
0.9
1.8
1.5
1.3
1.1

N195F + V206L + Y382I + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.8
1.7
1.1
0.9
2.1
2.0
1.3
1.1

N195F + V206L + S376H + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.7
1.5
1.1
0.9
2.1
2.1
1.2
1.3

N195F + V206L + I214E + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.2
1.9
1.1
1.1
2.4
2.0
1.4
1.3

N195F + V206L + I214W + K391A + G476K

H1* + N54S + V56T + K72R + G109A + Q172K + A174S +
1.7
1.4
0.9
1.0
2.1
1.7
1.2
1.1

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.9
1.9
1.1
1.1
2.7
2.1
1.5
1.3

N195F + A204T + V206L + D377H + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.1
1.6
1.2
1.1
2.2
1.8
1.3
1. text missing or illegible when filed

N195F + A204S + V206L + D377H + K391A + G476K

H1* + N54S + V56T + G109A + T165S + A174S + G182* +
1.9
1.8
1.1
1.1
2.3
2.0
1.4
1. text missing or illegible when filed

D183* + N195F + V206L + D377H + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.0
1.6
1.0
1.0
2.3
1.7
1.5
1. text missing or illegible when filed

N195F + V206L + T334S + D377H + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.0
1.6
1.0
1.0
2.1
1.6
1.4
1. text missing or illegible when filed

N195F + V206L + A263G + D377H + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.0
1.6
1.1
1.0
2.3
1.7
1.3
1. text missing or illegible when filed

N195F + V206L + M286F + D377H + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.9
1.6
1.0
1.0
1.8
1.7
1.3
1. text missing or illegible when filed

N195F + V206L + Y267I + D377H + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.1
1.8
1.1
1.0
2.5
2.1
1.5
1. text missing or illegible when filed

N195F + V206L + L235V + D377H + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.6
1.6
0.8
0.9
2.3
1.9
1.3
1.2

N195F + V206L + L235A + D377H + K391A + G476K

H1* + N54S + V56T + M105L + G109A + A174S + G182* +
2.1
1.9
1.1
1.1
2.9
2.1
1.5
1. text missing or illegible when filed

D183* + N195F + V206L + D377H + K391A + G476K

H1* + N54S + V56T + M105I + G109A + A174S + G182* +
2.2
2.0
1.1
1.1
2.3
2.0
1.6
1. text missing or illegible when filed

D183* + N195F + V206L + D377H + K391A + G476K

H1* + N54S + V56T + M105V + G109A + A174S + G182* +
2.3
2.1
1.2
1.1
2.6
2.1
1.5
1. text missing or illegible when filed

D183* + N195F + V206L + D377H + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.2
1.9
1.3
1.1
2.6
2.0
1.7
1. text missing or illegible when filed

N195F + V206L + M246I + D377H + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.3
2.0
1.3
1.1
2.3
1.9
1.6
1. text missing or illegible when filed

N195F + V206L + L250I + D377H + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.2
1.9
1.3
1.2
2.5
2.0
1.6
1. text missing or illegible when filed

N195F + V206L + L250V + D377H + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.2
1.9
1.2
1.1
2.1
1.9
1.6
1.4

N195F + V206L + K391Y + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.1
1.9
1.0
1.0
2.1
1.8
1.4
1.3

N195F + V206L + K391V + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.2
1.8
1.3
1.1
2.6
2.1
1.5
1.3

N195F + V206L + K391M + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.8
1.6
1.2
1.0
2.1
1.9
1.3
1.1

N195F + V206L + K391E + G476K

H1* + N54S + V56T + G109A + T165G + A174S + G182* +
2.3
2.0
1.3
1.1
2.9
2.0
1.7
1.3

D183* + N195F + V206L + D377H + K391A + G476K

H1* + N54R + V56T + G109A + A174S + G182* + D183* +
1.3
1.4
1.1
1.1
1.1
1.1
1.1
0.8

N195F + V206L + K391A + G476K

H1* + N54L + V56T + G109A + A174S + G182* + D183* +
1.6
1.4
1.1
1.0
1.3
1.6
1.3
1.2

N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109S + A174S + G182* + D183* +
2.4
2.4
1.4
1.2
2.3
2.0
1.4
1.3

N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174V + G182* + D183* +
2.2
2.2
1.3
1.3
2.3
2.0
1.5
1.4

N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.6
2.3
1.3
1.1
2.5
2.0
1.6
1.2

N195F + V206L + M246F + D377H + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.2
1.8
1.3
1.2
1.9
1.8
1.4
1.3

N195F + V206L + M246V + D377H + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.3
1.9
1.2
1.2
1.8
1.6
1.4
1.1

N195F + V206L + M246S + D377H + K391A + G476K

H1* + N54S + V56T + G109A + A174R + G182* + D183* +
1.7
1.6
1.2
1.1
1.7
1.6
1.4
1.2

N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174L + G182* + D183* +
1.9
1.7
1.2
1.2
2.2
1.7
1.3
1.2

N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.8
1.6
1.0
1.1
2.0
1.7
1.3
1.3

N195F + V206S + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.9
2.0
1.3
1.2
2.2
2.0
1.5
1.4

N195F + V206L + K391D + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.3
1.8
1.3
1.2
2.5
1.9
1.4
1.3

N195F + V206L + K391R + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.2
1.9
1.2
1.2
2.3
2.0
1.5
1.3

N195F + V206L + K391H + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.2
1.6
1.3
1.2
2.5
2.0
1.4
1. text missing or illegible when filed

N195F + V206L + K391W + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.1
1.8
1.2
1.2
2.0
1.8
1.3
1. text missing or illegible when filed

N195F + V206L + K391I + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.0
1.6
1.1
1.1
2.6
2.0
1.4
1. text missing or illegible when filed

N195F + V206L + L250T + D377H + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.1
1.8
1.0
1.0
2.6
2.0
1.5
1. text missing or illegible when filed

N195F + V206L + L250A + D377H + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.2
1.7
1.1
1.1
2.4
2.0
1.6
1. text missing or illegible when filed

N195F + V206L + L250F + D377H + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.2
1.7
1.1
1.1
2.0
1.9
1.4
1. text missing or illegible when filed

N195F + V206L + L250M + D377H + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.8
1.4
1.1
1.0
1.5
1.7
1.2
1. text missing or illegible when filed

N195F + V206L + K391Q + G476K

H1* + N54S + V56T + G109A + Q172H + L173A + A174P +
2.5
1.8
1.3
1.2
2.3
2.1
1.6
1.4

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + Q172E + L173A + A174R +
1.1
1.4
0.7
1.0
1.8
1.7
1.2
1. text missing or illegible when filed

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + K72Q + G109A + W167R + A174S +
1.9
1.7
1.2
1.1
1.6
1.6
1.0
1. text missing or illegible when filed

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.8
1.7
1.1
1.0
2.0
1.7
1.3
1. text missing or illegible when filed

G184T + N195F + V206L + G255A + K391A + G476K

H1* + N54S + V56T + K72R + G109A + A174S + G182* +
1.7
1.6
1.1
1.1
1.9
1.6
1.1
1. text missing or illegible when filed

D183* + G184T + N195F + V206L + D377H + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.4
2.1
1.3
1.2
2.3
2.0
1.4
1.2

G184T + N195F + V206L + G255A + D377H + K391A + G476K

H1* + N54S + V56T + G109A + Q118R + A174S + G182* +
1.6
1.5
1.1
1.2
0.7
1.1
0.8
1.0

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.2
2.0
1.3
1.2
2.3
1.8
1.3
1.3

N195F + V206L + S376T + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.0
1.8
1.2
1.2
2.1
1.8
1.4
1.3

N195F + V206L + S376V + K391A + G476K

H1* + N54S + V56T + G109A + Q172E + L173I + A174N +
2.3
1.9
1.1
1.1
2.1
1.9
1.1
1.2

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + Q172D + L173A + A174T +
2.3
2.0
1.1
1.1
2.2
2.0
1.1
1.2

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.1
2.0
1.2
1.2
2.6
2.0
1.5
1.3

N195F + V206L + Y267M + D377H + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.7
1.6
1.1
1.0
2.5
1.9
1.2
1.2

N195F + V206L + Y267H + D377H + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.6
1.5
1.1
1.0
2.3
1.7
1.2
1.1

N195F + V206L + V238A + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.5
1.3
1.1
0.9
1.7
1.5
1.2
1.1

N195F + V206L + V238T + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.3
1.7
1.2
1.1
3.0
2.1
1.4
1.3

N195F + V206L + V238G + K391A + G476K

H1* + N54S + V56T + M105V + G109A + A174S + G182* +
2.0
1.7
1.2
1.1
2.3
2.1
1.5
1.4

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.2
2.1
1.2
1.0
2.9
1.9
1.4
1.3

N195F + V206L + V264T + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.4
1.8
1.3
1.1
2.9
2.3
1.5
1.4

N195F + V206L + V264I + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.9
1.7
1.1
1.0
2.4
1.9
1.3
1.3

N195F + V206L + L235V + K391A + G476K

H1* + N54S + V56T + G109A + W167H + Q172N + L173A +
2.0
1.7
1.2
1.1
2.9
2.1
1.4
1.3

A174S + G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + W167F + Q172E + L173P +
1.7
1.9
1.2
1.1
3.3
2.2
1.5
1. text missing or illegible when filed

A174K + G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + Q172N + L173T + A174S +
2.0
1.7
1.2
1.1
2.8
2.0
1.4
1. text missing or illegible when filed

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + Q172K + L173V + A174T +
1.6
1.4
1.0
1.1
1.6
1.6
1.0
1. text missing or illegible when filed

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + Q172E + L173F + A174R +
1.9
1.6
1.1
1.0
2.0
1.9
1.3
1. text missing or illegible when filed

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + Q172E + L173T + A174R +
1.9
1.8
1.2
1.2
2.5
2.1
0.9
1. text missing or illegible when filed

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + Q172N + L173V + A174R +
1.9
1.7
1.3
1.2
2.2
1.8
0.9
1. text missing or illegible when filed

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + L173V + A174R + G182* +
1.7
1.8
1.3
1.2
1.7
1.6
0.8
1. text missing or illegible when filed

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + Q172E + L173M + A174H +
2.1
2.1
1.3
1.2
2.8
2.1
1.3
1.3

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + Q172E + L173F + A174P +
1.9
1.9
1.2
1.2
2.7
2.3
0.8
1. text missing or illegible when filed

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + W167Y + Q172E + L173V +
2.0
1.8
1.1
1.0
2.8
2.0
1.4
1. text missing or illegible when filed

A174H + G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + L173A + A174T + G182* +
2.0
1.8
1.0
1.0
2.4
1.8
1.4
1. text missing or illegible when filed

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.0
1.6
1.1
1.0
2.9
1.7
1.4
1. text missing or illegible when filed

N195F + V206L + V291A + F328L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.2
1.7
1.0
1.0
2.8
1.7
1.2
1. text missing or illegible when filed

N195F + V206L + M246L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.8
1.8
1.2
1.1
2.9
2.0
1.5
1.4

N195F + V206L + M246S + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.0
1.9
1.3
1.2
2.8
2.0
1.6
1.4

N195F + V206L + L250V + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.0
1.8
1.1
1.0
2.9
2.1
1.5
1.3

N195F + V206L + L250F + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.3
1.7
1.2
1.1
3.0
2.1
1.5
1.4

N195F + V206L + L250M + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.9
1.8
1.3
1.0
2.8
2.0
1.3
1.3

N195F + V206L + K391A + C474V + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.7
1.6
1.0
1.0
1.7
1.8
1.4
1.3

N195F + A204S + V206L + K391A + G476K

H1* + N54S + V56T + G109A + T165G + A174S + G182* +
2.3
1.9
1.2
1.1
2.9
2.2
1.3
1.3

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.9
1.7
1.0
1.0
2.6
2.0
1.4
1.3

N195F + V206L + T334S + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.1
1.3
1.0
0.9
1.4
1.3
1.1
1.1

N195F + V206L + A263G + M286F + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.7
1.5
1.1
1.0
2.2
1.9
1.3
1.1

N195F + V206L + A263G + M286L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.9
1.9
1.2
1.2
2.7
2.2
1.5
1.4

N195F + V206L + Y267H + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.9
1.8
1.0
1.1
2.4
2.2
1.5
1.4

N195F + V206L + Y267L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.2
1.5
0.7
0.9
1.7
1.9
1.3
1.3

N195F + V206L + Y267I + K391A + G476K

H1* + T51A + N54S + V56T + G109A + Q172R + A174S +
2.1
1.5
1.1
1.0
1.5
1.6
1.4
1.1

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.4
1.9
1.1
1.1
2.5
1.9
1.4
1.2

N195F + V206L + K391L + G476K

H1* + N54S + V56T + G109A + F113Q + R116H + Q172G +
2.6
2.1
1.1
1.0
3.1
2.1
1.4
1.2

A174S + G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + F113Q + Q172G + A174S +
1.9
1.8
0.9
0.9
2.7
1.9
1.2
1.1

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109S + F113Q + R116Q + A174S +
2.9
2.2
1.2
1.1
3.3
2.6
1.5
1. text missing or illegible when filed

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + Q172D + A174S + G182* +
2.7
2.2
1.2
1.1
3.1
2.1
1.5
1. text missing or illegible when filed

D183* + N195F + A204G + V206L + K391A + P473R + G476K

H1* + N54S + V56T + K72R + G109A + T134E + A174S +
2.8
2.3
1.2
1.1
3.4
2.4
1.7
1. text missing or illegible when filed

G182* + D183* + N195F + A204G + V206L + G255A +

K391A + P473R + G476K

H1* + N54S + V56T + K72R + G109A + W167F + A174S +
2.7
2.2
1.2
1.1
3.1
2.2
1.6
1. text missing or illegible when filed

G182* + D183* + N195F + A204G + V206L + K391A + G476K

H1* + N54S + V56T + K72R + G109A + A174S + G182* +
2.3
2.1
1.2
1.1
2.7
2.0
1.4
1. text missing or illegible when filed

D183* + G184T + N195F + A204G + V206L + K391A +

P473R + G476K

H1* + N54S + V56T + G109A + W167F + Q172N + A174S +
2.5
1.8
1.3
1.2
2.6
1.8
1.6
1. text missing or illegible when filed

G182* + D183* + N195F + V206L + K391A + P473R + G476K

H1* + N54S + V56T + G109A + W167F + Q172G + A174S +
2.4
2.0
1.3
1.2
2.8
2.0
1.6
1. text missing or illegible when filed

G182* + D183* + N195F + V206L + K391A + P473G + G476K

H1* + N54S + V56T + G109A + W167F + Q172G + A174S +
2.1
1.8
1.3
1.1
2.3
1.8
1.5
1.2

G182* + D183* + N195F + V206L + K391A + P473R + G476K

H1* + N54S + V56T + K72R + G109A + W167F + Q172R +
2.2
1.9
1.1
1.1
2.0
1.8
1.2
1.1

A174S + G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + K72R + G109A + R116H + W167F +
2.4
1.8
1.2
1.1
2.4
1.9
1.5
1.2

Q172R + A174S + G182* + D183* + N195F + V206L +

K391A + G476K

H1* + N54S + V56T + M105F + G109A + A174S + G182* +
2.6
2.2
1.3
1.3
3.4
2.4
1.5
1.4

D183* + N195F + V206L + R320A + S323N + K391A +

C474V + G476K

H1* + N54S + V56T + K72R + G109A + A174S + G182* +
2.4
2.1
1.4
1.3
3.2
2.2
1.7
1.3

D183* + N195F + V206L + G255A + K391A + C474V + G476K

H1* + N54S + V56T + G109A + T134E + A174S + G182* +
2.8
2.3
1.3
1.2
3.7
2.3
1.6
1.4

D183* + N195F + V206L + K391A + C474V + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.6
2.0
1.3
1.2
3.0
2.1
1.5
1.3

N195F + V206L + D377H + K391A + C474V + G476K

H1* + N54S + V56T + K72R + G109A + A174S + G182* +
2.8
2.1
1.3
1.2
3.3
2.4
1.5
1.4

D183* + N195F + V206L + K391A + C474V + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.1
1.9
1.0
1.1
2.6
2.0
1.5
1.3

N195F + V206L + A265G + K391A + T444Q + G476K + G477A

H1* + A37V + N54S + V56T + G109A + W167F + Q172G +
2.3
2.0
1.2
1.2
2.8
2.2
1.5
1.3

A174S + G182* + D183* + N195F + V206L + K391A +

G346T + G477A + G476K

H1* + N54S + V56T + G109A + R116Q + Q172D + A174S +
2.3
2.1
0.9
1.1
3.1
2.4
1.4
1.3

G182* + D183* + N195F + V206L + G346T + K391A +

T444Q + G477A + G476K

H1* + N54S + V56T + G109A + R116Q + W167F + Q172N +
2.2
2.2
1.1
1.2
2.7
2.4
1.3
1. text missing or illegible when filed

A174S + G182* + D183* + N195F + V206L + G346T +

K391A + T444Q + G477A + G476K

H1* + N54S + V56T + G109S + W167F + Q172R + A174S +
1.9
1.4
1.2
1.1
2.1
1.6
1.3
1. text missing or illegible when filed

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109S + R116Q + W167F + Q172N +
2.8
2.2
1.4
1.2
3.2
3.0
1.5
1. text missing or illegible when filed

A174S + G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109S + W167F + Q172G + A174S +
2.6
1.9
1.4
1.1
3.2
2.2
1.6
1. text missing or illegible when filed

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + F113Q + Q172G + A174S +
2.5
2.0
1.5
1.2
3.2
2.2
1.5
1.4

G182* + D183* + N195F + V206L + K391A + T444Q + G476K

H1* + N54S + V56T + G109A + F113Q + R116H + Q172G +
2.6
2.2
1.3
1.1
3.5
2.6
1.7
1. text missing or illegible when filed

A174S + G182* + D183* + N195F + V206L + K391A +

T444Q + G476K

H1* + N54S + V56T + G109A + F113Q + A174S + G182* +
2.5
2.0
1.3
1.1
3.1
2.6
1.6
1. text missing or illegible when filed

D183* + N195F + V206L + K391A + C474V + G476K

H1* + N54S + V56T + G109A + Q172N + A174S + G182* +
2.4
1.7
1.4
1.1
3.2
2.4
1.5
1. text missing or illegible when filed

D183* + N195F + V206L + V264I + K391A + C474V + G476K

H1* + N54S + V56T + G109A + Q172N + A174S + G182* +
2.5
1.7
1.3
1.1
2.0
2.1
1.3
1. text missing or illegible when filed

D183* + N195F + V206L + K391A + C474V + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.1
1.8
1.2
1.1
2.9
2.1
1.5
1.4

N195F + V206L + S280Q + H321Y + K391A + C474V + G476K

H1* + N54S + V56T + K72R + G109A + T134E + A174S +
2.1
1.8
1.2
1.0
3.3
2.3
1.6
1.4

G182* + D183* + N195F + V206L + G255A + K391A +

C474V + G476K

H1* + N54S + V56T + G109A + F113Q + R116H + Q172G +
2.4
2.0
1.2
1.1
3.3
2.3
1.7
1.3

A174S + G182* + D183* + N195F + V206L + K391A +

T444Q + P473R + G476K

H1* + N54S + V56T + G109A + F113Q + R116Q + Q172G +
2.8
2.1
1.3
1.1
3.1
2.3
1.5
1.3

A174S + G182* + D183* + N195F + V206L + K391A +

T444Q + G476K

H1* + N54S + V56T + G109A + R116H + W167F + Q172N +
2.4
2.0
1.2
1.1
2.8
2.2
1.5
1.3

A174S + G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + F113Q + R116H + T165G +
2.4
2.0
1.2
1.1
3.1
2.2
1.6
1.2

Q172G + A174S + G182* + D183* + N195F + V206L +

K391A + T444Q + G476K

H1* + N54S + V56T + G109A + F113Q + R116Q + T165G +
2.5
2.1
1.3
1.2
3.4
2.3
1.7
1.4

Q172G + A174S + G182* + D183* + N195F + V206L +

K391A + T444Q + G476K

H1* + N54S + V56T + G109A + F113Q + R116H + W167F +
2.3
1.8
1.2
1.1
2.6
2.0
1.4
1.3

Q172R + A174S + G182* + D183* + N195F + V206L +

F289I + K391A + G476K

H1* + N54S + V56T + G109A + F113Q + Q172N + A174S +
2.6
2.2
1.3
1.2
3.3
2.3
1.6
1.3

G182* + D183* + N195F + V206L + K391A + T444Q + G476K

H1* + N54S + V56T + G109A + F113Q + R116H + Q172N +
2.6
2.0
1.3
1.2
3.2
2.2
1.7
1. text missing or illegible when filed

A174S + G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + F113Q + R116Q + Q172M +
2.2
2.0
1.2
1.1
2.8
2.2
1.3
1. text missing or illegible when filed

A174S + G182* + D183* + N195F + V206L + K391A +

T444Q + G476K

H1* + N54S + V56T + G109A + F113Q + R116H + Q172M +
2.2
1.9
1.2
1.0
3.0
2.4
1.5
1. text missing or illegible when filed

A174S + G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + R116H + Q172M + A174S +
2.3
2.1
1.1
1.0
2.7
2.1
1.5
1. text missing or illegible when filed

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + F113Q + R116H + T165G +
1.9
1.7
1.2
1.1
2.6
1.8
1.5
1.3

Q172M + A174S + G182* + D183* + N195F + V206L +

K391A + Q395P + G476K

H1* + N54S + V56T + G109A + F113Q + A174S + G182* +
1.7
1.3
0.9
1.0
1.9
1.6
0.9
1. text missing or illegible when filed

D183* + N195F + V206L + K391A + T444Q + P473R + G476K

H1* + N54S + V56T + G109A + R116Q + A174S + G182* +
2.4
2.2
1.3
1.2
2.7
2.4
1.5
1. text missing or illegible when filed

D183* + N195F + V206L + A265G + K391A + T444Q +

P473R + G476K

H1* + N54S + V56T + G109A + R116Q + A174S + G182* +
2.4
2.1
1.3
1.1
2.9
2.3
1.3
1. text missing or illegible when filed

D183* + N195F + V206L + K391A + T444Q + P473R + G476K

H1* + N54S + V56T + K72R + G109A + T134E + T165G +
2.5
2.1
1.2
1.1
3.0
2.3
1.4
1.3

A174S + G182* + D183* + N195F + V206L + G255A +

K391A + G476K

H1* + N54S + V56T + G109A + F113Q + R116H + W167F +
2.1
2.0
1.2
1.2
2.9
2.3
1.5
1.3

Q172R + A174S + G182* + D183* + N195F + V206L +

K391A + G476K

H1* + N54S + V56T + G109A + R116Q + W167F + Q172R +
2.0
1.8
1.2
1.1
2.2
2.0
1.3
1.3

A174S + G182* + D183* + N195F + V206L + A265G +

K391A + P473R + G476K

H1* + N54S + V56T + G109A + F113Q + A174S + G182* +
2.1
1.9
1.0
1.0
2.6
2.0
1.4
1.2

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + Q172N + A174S + G182* +
2.0
1.7
1.2
1.1
2.5
1.9
1.5
1.1

D183* + N195F + V206L + K391A + G476K

H1* + T51V + N54S + V56T + G109A + Q172D + A174S +
2.4
1.9
1.2
1.1
2.6
1.9
1.6
1.2

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.3
1.9
1.2
1.1
2.5
1.8
1.5
1.2

N195F + V206L + S280Q + H321Y + K391A + G476K

H1* + N54S + V56T + G109A + F113Q + R116H + Q172N +
2.6
2.3
1.3
1.2
3.1
2.4
1.7
1.4

A174S + G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + F113Q + R116H + Q172G +
2.3
2.0
1.3
1.2
2.9
2.3
1.6
1.3

L173V + A174S + G182* + D183* + N195F + V206L +

K391A + T444Q + P473R + G476K

H1* + N54S + V56T + G109A + R116Q + W167F + Q172N +
2.6
2.2
1.3
1.1
3.3
2.5
1.8
1.3

L173V + A174S + G182* + D183* + N195F + V206L +

K391A + T444Q + G476K

H1* + N54S + V56T + G109A + G133Q + W167F + Q172N +
2.5
2.3
1.3
1.2
3.3
2.4
1.8
1.4

A174S + G182* + D183* + N195F + V206L + Q395P +

K391A + G476K

H1* + N54S + V56T + G109A + F113Q + R116H + W167F +
2.3
2.0
1.2
1.2
2.8
2.3
1.6
1. text missing or illegible when filed

Q172N + L173V + A174S + G182* + D183* + N195F +

V206L + K391A + G476K

H1* + A37V + N54S + V56T + K72R + G109A + R116H +
2.5
2.2
1.3
1.1
3.3
2.5
1.7
1. text missing or illegible when filed

W167F + Q172R + A174S + G182* + D183* + G184T +

N195F + V206L + K391A + P473R + G476K

H1* + N54S + V56T + K72R + G109A + F113Q + R116H +
2.2
2.2
1.2
1.1
3.1
2.4
1.7
1. text missing or illegible when filed

W167F + Q172G + L173V + A174S + G182* + D183* +

G184T + N195F + V206L + K391A + T444Q + P473R + G476K

H1* + N54S + V56T + G109S + R116Q + W167F + Q172R +
2.5
2.2
1.2
1.0
3.1
2.5
1.6
1. text missing or illegible when filed

A174S + G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109S + R116Q + W167F + Q172G +
2.5
2.1
1.2
1.1
3.0
2.3
1.6
1.2

A174S + G182* + D183* + N195F + V206L + K391A +

P473R + G476K

H1* + N54S + V56T + G109S + F113Q + W167F + Q172N +
2.7
2.4
1.4
1.3
3.1
2.5
1.7
1. text missing or illegible when filed

A174S + G182* + D183* + N195F + V206L + K391A + G476K

H1* + A37V + N54S + V56T + G109S + F113Q + W167F +
2.8
2.4
1.3
1.2
3.5
2.5
1.7
1. text missing or illegible when filed

Q172R + A174S + G182* + D183* + N195F + V206L +

K391A + G476K

H1* + N54S + V56T + K72R + G109A + R116H + T134E +
2.2
1.9
1.2
1.1
2.7
2.4
1.6
1. text missing or illegible when filed

W167F + Q172G + L173V + A174S + G182* + D183* +

N195F + V206L + G255A + K391A + G476K

H1* + N54S + V56T + K72R + G109A + R116H + T134E +
2.5
2.0
1.2
1.1
3.5
2.5
1.6
1.3

W167F + Q172G + L173V + A174S + G182* + D183* +

N195F + V206L + G255A + K391A + Q395P + T444Q +

P473R + G476K

H1* + N54S + V56T + K72R + G109A + F113Q + R116Q +
2.5
2.1
1.3
1.1
3.4
2.5
1.6
1.3

W167F + Q172G + A174S + G182* + D183* + G184T +

N195F + V206L + K391A + P473R + G476K

H1* + N54S + V56T + K72R + G109A + F113Q + R116Q +
2.3
2.0
1.1
1.1
3.1
2.6
1.5
1.3

T134E + W167F + Q172R + A174S + G182* + D183* +

N195F + V206L + G255A + K391A + T444Q + P473R + G476K

H1* + N54S + V56T + K72R + G109A + F113Q + T134E +
1.9
1.9
1.1
1.1
2.7
2.1
1.6
1.3

W167F + Q172R + A174S + G182* + D183* + N195F +

V206L + G255A + K391A + Q395P + A445Q + P473R + G476K

H1* + N54S + V56T + K72R + G109A + F113Q + R116H +
2.1
2.2
1.3
1.2
2.9
2.4
1.6
1.4

T134E + W167F + Q172R + A174S + G182* + D183* +

N195F + V206L + G255A + K391A + Q395P + G476K

H1* + N54S + V56T + K72R + G109A + T134E + W167F +
2.6
2.4
1.3
1.2
3.2
2.5
1.7
1.4

Q172N + A174S + G182* + D183* + N195F + V206L +

G255A + A265G + K391A + T444Q + P473R + G476K

H1* + N54S + V56T + K72R + G109A + G133Q + W167F +
2.4
2.2
1.3
1.2
3.2
2.4
1.6
1.4

Q172R + A174S + G182* + D183* + N195F + V206L +

K391A + G476K

H1* + N54S + V56T + K72R + G109A + F113Q + R116Q +
2.9
2.3
1.3
1.2
3.7
2.8
1.8
1.4

W167F + Q172G + A174S + G182* + D183* + N195F +

A204G + V206L + K391A + G476K

H1* + N54S + V56T + K72R + G109A + F113Q + R116Q +
2.0
2.0
1.3
1.2
2.7
2.1
1.5
1.3

W167F + Q172N + L173V + A174S + G182* + D183* +

G184T + N195F + V206L + K391A + T444Q + P473R + G476K

H1* + A37H + N54S + V56T + G109A + F113Q + W167F +
2.5
1.9
1.2
1.1
2.6
1.8
1.6
1. text missing or illegible when filed

Q172N + A174S + G182* + D183* + N195F + V206L +

K391A + G476K

H1* + A37H + N54S + V56T + G109A + F113Q + A174S +
1.9
1.7
1.3
1.2
2.8
2.0
1.3
1. text missing or illegible when filed

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + F113Q + W167F + Q172N +
2.3
1.6
1.3
1.2
2.8
1.8
1.5
1. text missing or illegible when filed

A174S + G182* + D183* + N195F + V206L + K391A +

T459N + G476K

H1* + N54S + V56T + G109A + F113Q + Q172R + L173V +
1.9
1.5
1.1
1.0
2.6
1.5
1.3
1. text missing or illegible when filed

A174S + G182* + D183* + N195F + V206L + K391A +

T444Q + G476K

H1* + N54S + V56T + G109A + F113Q + R116Q + A174S +
2.3
2.2
1.1
1.1
3.2
2.3
1.4
1.2

G182* + D183* + N195F + V206L + K391A + P473R + G476K

H1* + N54S + V56T + A60V + G109A + R116Q + W167F +
2.4
2.2
1.2
1.2
3.3
2.4
1.3
1. text missing or illegible when filed

Q172N + A174S + G182* + D183* + N195F + V206L +

K391A + G476K

H1* + N54S + V56T + A60V + G109A + R116H + Q172R +
2.7
2.0
1.4
1.2
3.2
2.5
1.3
1. text missing or illegible when filed

L173V + A174S + G182* + D183* + N195F + V206L +

K391A + G476K

H1* + A37V + N54S + V56T + A60V + G109A + R116Q +
2.5
1.9
1.1
1.2
2.9
2.1
1.1
1. text missing or illegible when filed

T165G + A174S + G182* + D183* + N195F + V206L +

K391A + G476K

H1* + A37H + N54S + V56T + A60V + G109A + R116Q +
2.7
2.2
1.2
1.2
3.6
2.5
1.7
1.4

T165G + A174S + G182* + D183* + N195F + V206L +

K391A + G476K

H1* + N54S + V56T + A60V + G109A + R116Q + W167F +
2.3
2.3
1.0
1.2
2.5
2.3
1.2
1.3

Q172G + L173V + A174S + G182* + D183* + N195F +

V206L + K391A + G476K

H1* + N54S + V56T + A60V + G109A + R116Q + A174S +
2.3
2.1
0.9
1.1
3.1
2.4
1.4
1.3

G182* + D183* + N195F + V206L + K391A + G476K

H1* + A37H + N54S + V56T + A60V + G109A + R116Q +
3.0
2.4
1.2
1.1
3.6
2.4
1.7
1.3

W167F + Q172R + A174S + G182* + D183* + N195F +

V206L + K391A + G476K

H1* + A37H + N54S + V56T + A60V + G109A + R116Q +
2.7
2.7
1.1
1.1
3.0
2.4
1.4
1.3

W167F + Q172N + A174S + G182* + D183* + N195F +

V206L + K391A + G476K

H1* + N54S + V56T + A60V + G109A + R116Q + T165G +
2.4
2.2
1.2
1.2
3.0
2.2
1.3
1.2

A174S + G182* + D183* + N195F + V206L + K391A +

T444Q + P473R + G476K

H1* + N54S + V56T + A60V + G109A + R116Q + A174S +
2.5
1.8
0.9
1.0
2.2
2.0
1.1
1.2

G182* + D183* + N195F + V206L + K391A + T444Q +

P473R + G476K

H1* + N54S + V56T + A60V + G109A + R116Q + W167F +
2.7
2.8
1.0
1.1
3.3
2.7
1.6
1.4

Q172N + L173V + A174S + G182* + D183* + N195F +

V206L + K391A + G476K

H1* + N54S + V56T + A60V + G109A + R116Q + W167F +
2.8
2.5
1.3
1.2
3.6
2.7
1.7
1.4

Q172R + A174S + G182* + D183* + N195F + V206L +

K391A + G476K

H1* + N54S + V56T + G109A + R116A + W167F + Q172R +
2.3
2.0
1.1
1.1
2.5
2.0
1.6
1. text missing or illegible when filed

A174S + G182* + D183* + N195F + A204G + V206L +

K391A + P473R + G476K

H1* + N54S + V56T + G109A + R116A + A174S + G182* +
2.8
2.5
1.3
1.2
3.4
2.3
1.6
1. text missing or illegible when filed

D183* + N195F + A204G + V206L + K391A + P473R +

G476K

H1* + N54S + V56T + G109A + R116A + T165G + A174S +
2.5
2.1
1.2
1.1
2.8
2.0
1.6
1. text missing or illegible when filed

G182* + D183* + N195F + V206L + K391A + T444Q +

P473R + G476K

H1* + N54S + V56T + G109A + G182* + D183* +
2.2
2.2
1.2
1.1
2.3
2.1
1.5
1. text missing or illegible when filed

N195F + K391A + G476K

H1* + N54S + V56T + G109A + G182* + D183* +
2.2
2.0
1.3
1.2
2.4
1.9
1.5
1. text missing or illegible when filed

N195F + V206L

N54S + V56T + G109A + G182* + D183* + N195F
2.2
2.0
1.3
1.2
2.3
2.0
1.5
1.4

N54S + V56T + G109A + A174S + G182* + D183* + N195F +
2.4
2.0
1.3
1.2
2.5
2.0
1.5
1.4

V206L + K391A + G476K

H1* + G109A + A174S + G182* + D183* + N195F + V206L +
1.9
1.9
1.3
1.2
2.4
2.0
1.5
1. text missing or illegible when filed

K391A + G476K

H1* + N54S + V56T + G109A + G182* + D183* + N195F +
2.2
1.9
1.2
1.1
2.5
1.7
1.3
1. text missing or illegible when filed

V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.0
1.9
1.2
1.1
2.2
1.8
1.5
1. text missing or illegible when filed

N195F + V206L + K391A

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.0
1.9
1.2
1.1
2.5
1.9
1.4
1. text missing or illegible when filed

N195F + G476K

H1* + N54S + V56T + A174S + G182* + D183* + N195F +
2.3
2.0
1.2
1.1
2.6
2.1
1.4
1. text missing or illegible when filed

V206L + G273R + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.4
2.1
1.3
1.1
2.7
2.1
1.6
1. text missing or illegible when filed

N195F + V206L

H1* + W48G + G109A + G182* + D183* + N195F + V206L +
1.7
1.6
1.2
1.1
2.2
1.7
1.3
1.2

K391A + G476K

N54S + V56T + G109A + A174S + G182* + D183* + N195F +
2.0
1.7
1.2
1.2
2.9
2.2
1.5
1.3

K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.4
1.5
1.1
1.1
2.3
1.8
1.3
1.3

N195F + K391A

H1* + N54S + V56T + G109A + F113Q + Q172M + A174S +
2.0
1.9
1.2
1.1
2.9
2.2
1.3
1.2

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + R116Q + A174S + G182* +
1.8
1.6
0.9
1.0
1.7
1.8
1.0
1.1

D183* + N195F + A204G + V206L + K391A + G476K

H1* + N54S + V56T + K72R + G109A + F113Q + T134E +
2.5
2.2
1.2
1.2
2.9
2.5
1.4
1.4

A174S + G182* + D183* + N195F + V206L + G255A +

K391A + G476K

H1* + N54S + V56T + K72R + G109A + F113Q + T134E +
2.7
2.3
1.3
1.2
3.3
2.5
1.7
1.4

A174S + G182* + D183* + N195F + V206L + G255A +

K391A + P473R + G476K

H1* + N54S + V56T + K72R + G109A + R116Q + A174S +
2.7
2.2
1.2
1.2
3.4
2.4
1.6
1.3

G182* + D183* + G184T + N195F + V206L + K391A +

G476K

H1* + A37V + N54S + V56T + G109A + R116A + T165G +
2.4
2.2
1.2
1.2
3.1
2.5
1.5
1.3

A174S + G182* + D183* + N195F + V206L + K391A +

G476K

H1* + A37V + N54S + V56T + G109A + R116Q + T165G +
2.5
2.2
1.2
1.2
3.0
2.3
1.5
1.3

A174S + G182* + D183* + N195F + V206L + M246F +

K391A + G476K

H1* + V56A + G109A + A174S + G182* + D183* +
2.0
2.0
1.1
1.1
2.5
2.1
1.5
1.3

N195F + V206L + G476K

H1* + N54S + V56T + G109A + G182* + D183* +
1.8
2.0
1.2
1.0
2.2
1.9
1.6
1.4

N195F + V206L + K391A

H1* + N54S + V56T + G109A + G182* + D183* +
1.8
1.7
1.0
1.1
1.9
1.9
1.4
1.3

N195F + V206L + G476K

G109A + A174S + G182* + D183* + N195F + V206L +
1.5
1.6
1.0
1.0
1.7
1.7
1.2
1. text missing or illegible when filed

K391A + G476K

N54S + V56T + G109A + G182* + D183* + N195F +
1.7
1.7
1.2
1.0
1.6
1.7
1.4
1. text missing or illegible when filed

V206L + K391T + G476K

H1* + N54S + V56T + K72R + G109A + F113Q + R116H +
2.4
2.2
1.2
1.2
3.4
2.3
1.4
1. text missing or illegible when filed

A174S + G182* + D183* + G184T + N195F + V206L +

K391A + T444Q + G476K

H1* + N54S + V56T + G109S + F113Q + A174S + G182* +
2.4
2.0
1.3
1.2
3.1
2.2
1.6
1. text missing or illegible when filed

D183* + N195F + V206L + K391A + T444Q + G476K

H1* + N54S + V56T + G109A + F113Q + R116H + W167F +
2.9
2.3
1.3
1.2
3.8
2.6
1.8
1. text missing or illegible when filed

Q172G + L173V + A174S + G182* + D183* + N195F +

V206L + K391A + P473R + G476K

H1* + N54S + V56T + G109A + F113Q + R116Q + Q172N +
2.8
2.3
1.2
1.2
3.5
2.6
1.7
1. text missing or illegible when filed

A174S + G182* + D183* + N195F + V206L + A265G +

K391A + P473R + G476K

H1* + N54S + V56T + G109A + F113Q + W167F + Q172N +
2.5
2.0
1.2
1.2
2.9
2.2
1.7
1. text missing or illegible when filed

A174S + G182* + D183* + N195F + A204G + V206L +

K391A + P473R + G476K

H1* + N54S + V56T + G109A + F113Q + R116H + W167F +
2.1
1.9
1.2
1.1
2.3
1.8
1.3
1. text missing or illegible when filed

Q172R + A174S + G182* + D183* + N195F + A204G +

V206L + K391A + P473R + G476K

H1* + N54S + V56T + G109A + R116A + A174S + G182* +
2.1
1.9
1.1
1.1
2.7
2.2
1.5
1.3

D183* + N195F + V206L + K391A + T444Q + P473R +

G476K

H1* + N54S + V56T + G109A + R116H + Q172R + A174S +
2.2
1.6
1.2
1.1
2.5
1.8
1.5
1.2

G182* + D183* + N195F + A204G + V206L + K391A +

P473R + G476K

H1* + A37V + N54S + V56T + G109A + R116Q + T165G +
2.3
2.0
1.2
1.1
3.0
2.1
1.5
1.4

A174S + G182* + D183* + N195F + V206L + K391A +

G476K

H1* + A37H + N54S + V56T + G109A + R116H + T165G +
2.3
2.0
1.3
1.1
3.2
2.2
1.5
1.4

A174S + G182* + D183* + N195F + V206L + M246F +

K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.0
1.9
1.1
1.1
2.1
1.9
1.2
1.2

N195F + K391A + G476K

H1* + G109A + G182* + D183* + N195F + V206L +
1.7
1.6
1.1
1.1
1.8
1.5
1.3
1.2

K391A + G476K

G109A + G182* + D183* + N195F + V206L + G476K
1.9
1.7
1.2
1.1
2.1
1.5
1.2
1.1

H1* + G109A + G182* + D183* + N195F + K391A
1.9
1.9
1.2
1.2
1.6
1.7
1.3
1.3

H1* + N54S + V56T + G109A + G133S + A174S + G182* +
2.2
1.9
1.2
1.1
2.4
2.0
1.3
1.3

D183* + N195F + K391A + G476H

H1* + N54S + V56T + G109A + R116A + Q172N + L173V +
2.5
2.3
1.2
1.2
3.2
2.5
1.6
1.4

A174S + G182* + D183* + N195F + V206L + K391A +

G476K

H1* + A37V + N54S + V56T + G109A + R116H + T165G +
2.5
2.1
1.1
1.2
2.6
2.3
1.4
1.3

A174S + G182* + D183* + N195F + V206L + K391A +

G476K

H1* + P45A + N54S + V56T + G109A + A174S + G182* +
2.3
2.1
1.3
1.2
3.1
2.1
1.5
1.4

D183* + N195F + V206L + K391A + C474V + G476K

H1* + N54S + V56T + G109A + P124A + A174S + G182* +
1.9
2.2
1.3
1.2
2.8
2.2
1.4
1.3

D183* + N195F + V206L + K391A + C474V + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.0
2.0
1.3
1.2
2.4
2.1
1.5
1. text missing or illegible when filed

N195F + V206L + P364A + K391A + C474V + G476K

H1* + N54S + V56T + G109A + Q118R + T134E + A174S +
3.7
2.5
1.6
1.2
3.2
2.6
1.5
1. text missing or illegible when filed

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
3.4
2.4
1.6
1.3
3.9
2.7
1.6
1. text missing or illegible when filed

G184T + N195F + V206L + K391A + G476K

H1* + G50A + T51A + N54S + V56T + G109A + A174S +
3.2
2.2
1.5
1.2
3.1
2.4
1.8
1. text missing or illegible when filed

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
3.2
2.3
1.6
1.3
3.4
2.3
1.7
1. text missing or illegible when filed

N195F + V206L + V264F + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
3.0
2.2
1.6
1.2
2.9
2.4
1.4
1. text missing or illegible when filed

N195F + V206L + Y382L + K391A + G476K

H1* + G7K + N54S + V56T + G109A + A174S + G182* +
3.7
2.2
1.5
1.1
3.7
2.6
1.7
1. text missing or illegible when filed

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
3.0
2.2
1.5
1.2
3.2
2.6
1.7
1.5

N195F + V206L + G255A + Q256A + K391A + G476K

H1* + N54S + V56T + G109A + W167Y + A174S + G182* +
3.4
2.3
1.4
1.3
3.0
2.4
1.8
1. text missing or illegible when filed

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + W167H + A174S + G182* +
2.9
2.0
1.4
1.1
2.5
2.1
1.8
1. text missing or illegible when filed

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + T165V + A174S + G182* +
2.6
2.0
1.4
1.2
2.5
2.2
1.6
1. text missing or illegible when filed

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
3.2
2.4
1.3
1.2
3.0
2.1
1.9
1. text missing or illegible when filed

N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174N + G182* + D183* +
2.8
2.2
1.4
1.2
2.7
1.8
1.4
1. text missing or illegible when filed

N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174Q + G182* + D183* +
3.5
2.0
1.6
1.2
2.4
2.1
1.8
1. text missing or illegible when filed

N195F + V206L + K391A + G476K

H1* + N16H + V17L + N54S + V56T + G109A + A174S +
2.7
1.9
1.3
1.2
4.6
2.3
1.6
1.4

G182* + D183* + N195F + V206L + Y382F + K391A +

G476K

H1* + Q32S + N54S + V56T + G109A + A174S + G182* +
2.7
1.8
1.3
1.1
3.5
2.2
1.4
1.3

D183* + N195F + V206L + Q385L + K391A + G476K

H1* + N54S + V56T + R87S + G109A + R171H + A174S +
2.5
1.9
1.1
1.1
3.4
2.1
1.4
1.4

G182* + D183* + N195F + V206L + K391A + G476K

N54S + G182* + D183* + N195F
1.1
1.1
1.1
1.0
1.6
1.5
1.0
1.0

G109A + G182* + D183* + N195F
1.6
1.4
1.3
1.0
1.8
1.4
1.0
1.1

A174S + G182* + D183* + N195F
0.8
1.1
1.3
0.9
1.2
1.2
0.9
1.0

G182* + D183* + N195F + V206L
0.7
0.7
1.0
1.0
0.9
1.3
0.8
1.0

G182* + D183* + N195F + K391A
1.6
1.4
1.3
1.0
1.4
1.5
1.0
1.1

G182* + D183* + N195F + G476K
1.3
1.4
1.3
1.0
1.8
1.5
1.1
1.0

H1* + N54S + V56T + G109A + R116H + Q169E + Q172K +
3.5
1.9
1.2
1.1
3.4
2.2
1.6
1.2

A174* + G182* + D183* + N195F + V206L + K391A +

G476K

H1* + N54S + V56T + G109A + A174* + G182* + D183* +
3.4
2.1
1.3
1.1
3.2
2.1
1.5
1.1

N195F + V206L + K391A + G476K

H1* + K72R + G109A + F113Q + R116Q + W167F + Q172G +
3.1
2.0
1.1
1.0
3.0
2.1
1.7
1.4

A174S + G182* + D183* + G184T + N195F +

V206L + K391A + P473R + G476K

H1* + N54S + V56T + K72R + G109A + F113Q + R116Q +
3.1
1.9
1.2
1.0
3.0
1.9
1.6
1.3

W167F + Q172G + A174S + G182* + D183* + G184T +

N195F + V206L + P473R + G476K

H1* + N54S + V56T + G109A + Q169E + Q172G + A174* +
3.0
1.9
1.1
1.0
2.7
2.0
1.3
1.2

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + K72R + G109A + F113Q + R116Q +
2.9
1.8
1.1
1.0
2.9
1.9
1.4
1.1

W167F + Q172G + A174S + G182* + D183* + G184T +

N195F + K391A + P473R + G476K

H1* + N54S + V56T + G109A + R116W + Q169E + Q172K +
2.8
1.9
1.1
1.0
3.2
2.1
1.5
1.1

A174* + G182* + D183* + N195F + V206L + K391A +

G476K

H1* + N54S + V56T + G109A + Q169E + Q172K + A174* +
2.7
1.8
1.3
1.0
3.3
2.0
1.7
1.2

G182* + D183* + N195F + V206F + K391A + G476K

H1* + V56T + G109A + A174S + G182* + D183* + N195F +
2.6
1.6
1.2
1.1
2.4
1.6
1.4
1.1

V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.6
1.7
1.2
1.0
3.4
2.2
1.4
1.1

N195F + V206L + N260G + K391A + G476K

H1* + N54S + V56T + K72R + G109A + A174S + G182* +
2.6
1.5
1.2
1.0
3.1
1.8
1.6
1.2

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + Q169E + Q172K + A174* +
2.5
1.8
1.2
1.0
2.2
1.7
1.6
1.2

G182* + D183* + G184T + N195F + V206L + K391A +

G476K

H1* + N54S + V56T + G109A + Q169E + Q172K + A174* +
2.4
1.6
1.1
1.0
2.6
1.9
1.5
1.2

G182* + D183* + N195F + V206L + Y295N + K391A +

G476K

H1* + N54S + V56T + G109A + W167F + A174S + G182* +
2.4
1.7
1.2
1.0
2.5
1.9
1.7
1.3

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + Q169E + Q172K + A174* +
2.4
1.6
1.4
1.0
4.9
1.8
1.8
1.2

G182* + D183* + A186N + N195F + V206L + K391A +

G476K

H1* + N54S + V56T + G109A + Q169E + Q172K + A174* +
2.4
1.6
1.1
1.0
2.4
1.6
1.3
1.1

G182* + D183* + N195F + V206L + A288V + K391A +

G476K

H1* + N54S + V56T + G109A + Q169E + Q172K + A174* +
2.4
1.9
1.2
1.0
3.2
2.2
2.0
1.3

G182* + D183* + N195F + V206L + G255A + K391A +

G476K

H1* + N54S + G109A + Q169E + Q172K + A174* + G182* +
2.3
1.7
1.1
1.1
3.2
2.2
1.5
1.2

D183* + N195F + V206L + K391A + G476K

H1* + W48F + N54S + V56T + G109A + Q169E + Q172K +
2.3
1.6
1.1
1.0
2.3
1.6
1.4
1.2

A174* + G182* + D183* + N195F + V206L + K391A +

G476K

H1* + N54S + V56T + G109A + Q169E + Q172K + A174* +
2.3
1.8
1.2
1.1
1.8
1.8
1.6
1.2

G182* + D183* + N195F + V206L + V291A + K391A +

G476K

H1* + N54S + V56T + G109A + F113Q + A174S + G182* +
2.3
1.7
1.3
1.0
2.3
2.0
1.7
1.3

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + Q169E + Q172K + A174* +
2.3
1.6
1.2
1.0
3.3
2.0
1.6
1.2

G182* + D183* + N195F + V206L + R320A + S323N +

K391A + G476K

H1* + V56T + G109A + Q169E + Q172K + A174* + G182* +
2.3
1.6
1.2
0.9
2.0
1.7
1.6
1.1

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + K72R + G109A + F113Q + R116H +
2.3
1.7
1.1
1.0
2.0
1.7
1.7
1.2

W167F + Q172G + A174S + G182* + D183* + G184T +

N195F + V206L + K391A + P473R + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.2
1.7
1.1
1.0
2.6
2.1
1.8
1.4

N195F + V206L + N270T + K391A + G476K

H1* + N54S + V56T + G109A + Q169E + Q172K + A174* +
2.2
1.6
1.2
1.1
2.4
1.6
1.3
1.2

G182* + D183* + N195F + V206L + G476K

H1* + N54S + V56T + G109A + Q169E + Q172K + A174* +
2.2
1.6
1.1
1.0
3.2
2.0
1.7
1.2

G182* + D183* + N195F + V206L + N260G + K391A +

G476K

H1* + N54S + V56T + G109A + F113Q + R116H + A174S +
2.2
1.7
1.1
1.1
3.4
2.1
1.4
1.2

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + Q172G + A174S + G182* +
2.2
1.8
1.2
1.1
2.3
1.9
1.6
1.3

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + A186D + G182* +
2.1
1.7
1.2
1.0
2.2
1.7
1.5
1.2

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + Q169E + Q172K + A174* +
2.1
1.8
1.0
0.9
2.0
2.0
1.9
1.3

G182* + D183* + N195F + V206L + A225V + K391A +

G476K

H1* + G109A + Q169E + Q172K + A174* + G182* +
2.1
1.5
1.1
0.9
1.8
1.4
1.3
1.1

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + Q169E + Q172K + A174* +
2.1
1.6
1.2
1.0
3.3
2.1
1.6
1.2

G182* + D183* + N195F + V206L + G255S + K391A +

G476K

H1* + N54S + V56T + G109A + Q169E + Q172K + A174* +
2.1
1.7
1.0
1.1
2.0
1.8
1.4
1.3

G182* + D183* + N195F + V206L + K269Q + K391A +

G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.1
1.5
1.1
1.0
2.0
1.5
1.2
1.0

N195F + V206L + G255S + K391A + G476K

H1* + N54S + V56T + G109A + Q169E + Q172K + A174S +
2.1
1.6
1.1
1.1
2.9
2.1
1.5
1.3

G182* + D183* + N195F + V206L + K391A + G476K

G182* + D183* + N195F
2.0
1.7
1.2
0.9
2.9
1.8
1.8
1.2

H1* + N54S + V56T + G109A + Q169E + A174S + G182* +
2.0
1.6
1.1
1.1
2.0
1.8
1.6
1.3

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + Q169E + Q172K + A174* +
2.0
1.5
1.1
1.0
2.8
1.9
1.4
1.2

G182* + D183* + N195F + V206L + W284H + K391A +

G476K

H1* + N54S + G109A + A174S + G182* + D183* +
2.0
1.5
1.1
1.0
2.3
1.8
1.4
1.2

N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + Q169E + Q172K + A174* +
2.0
1.5
1.4
1.0
5.8
1.7
1.9
1.3

G182* + D183* + N195F + V206L + S304R + K391A +

G476K

H1* + N54S + V56T + K72R + G109A + F113Q + R116Q +
2.0
1.6
1.2
1.1
2.0
1.6
1.3
1.1

W167F + Q172G + A174S + G182* + D183* + G184T +

N195F + V206L + K391A + P473R

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.0
1.7
1.2
1.1
1.9
1.9
1.5
1.3

N195F + V206L + M246V + K391A + G476K

H1* + N54S + V56T + G109A + Q169E + Q172K + A174* +
1.9
1.4
1.2
1.1
2.7
2.0
1.4
1.2

G182* + D183* + N195F + V206L + K391A

H1* + N54S + V56T + G109A + Q169E + Q172K + A174* +
1.9
1.5
1.1
1.0
2.2
1.7
1.4
1.1

G182* + D183* + N195F + V206L + N270G + K391A +

G476K

H1* + N54S + V56T + G109A + Q169E + Q172K + A174* +
1.9
1.6
1.2
1.0
2.7
1.8
1.4
1.2

G182* + D183* + N195F + V206L + Y295F + K391A +

G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.9
1.5
1.2
1.1
2.9
1.8
1.3
1.3

N195F + V206L + R320A + K391A + G476K

H1* + N54S + V56T + G109A + Q169E + Q172K + A174T +
1.9
1.9
1.2
1.0
2.4
2.1
1.6
1.4

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + Q169E + Q172K + G182* +
1.9
1.7
1.1
1.0
2.6
1.7
1.8
1.2

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + W140Y + Q169E + Q172K +
1.9
1.4
1.1
1.0
2.1
1.8
1.5
1.4

A174* + G182* + D183* + N195F + V206L + K391A +

G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.9
1.6
1.1
1.1
2.2
1.9
1.7
1.4

N195F + V206L + K391A

H1* + N54S + V56T + G109A + Q169E + Q172K + A174* +
1.8
1.6
1.1
1.0
1.7
1.4
1.5
1.1

G182* + D183* + N195F + V206L + F328L + K391A +

G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.8
2.0
1.5
1.1
5.2
2.0
1.8
1.5

N195F + V206L + G476K

F113Q + R116H + D183* + G182* + N195F
1.8
1.7
1.1
1.0
1.9
1.9
1.7
1.3

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.8
1.3
1.1
1.0
2.6
1.8
1.5
1.2

N195F + V206L + A288L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.8
1.6
1.2
1.1
1.9
1.8
1.6
1.2

N195F + V206L + N270G + K391A + G476K

Q169E + Q172K + A174* + D183* + G182* + N195F
1.8
1.2
1.2
1.0
3.3
1.4
1.5
1.3

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.8
1.0
1.1
0.9
1.8
1.4
1.5
1.1

N195F + V206L + K269Q + K391A + G476K

H1* + W48F + N54S + V56T + G109A + A174S + G182* +
1.8
1.4
1.1
1.0
2.2
1.7
1.5
1.2

D183* + N195F + V206L + K391A + G476K

F113Q + R116Q + D183* + G182* + N195F
1.8
1.7
1.1
1.0
2.1
1.7
1.7
1.4

N54S + V56T + D183* + G182* + N195F
1.8
1.2
0.8
0.9
1.3
1.3
1.0
1.0

H1* + N54S + V56T + G109A + A174T + G182* + D183* +
1.7
1.7
1.2
1.1
2.4
1.9
1.8
1.3

N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + R116W + A174S + G182* +
1.7
1.6
1.2
1.1
1.8
1.8
1.6
1.2

D183* + N195F + V206L + K391A + G476K

Q169E + Q172K + G182* + D183* + N195F
1.7
1.4
1.3
1.0
4.9
1.6
1.8
1.3

G182* + D183* + A186D
1.7
1.6
1.0
1.0
1.6
1.7
1.3
1.1

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.7
1.5
1.1
1.0
1.8
1.6
1.4
1.1

N195F + V206L + V238A + K391A + G476K

G182* + D183* + A186N
1.7
1.8
1.2
1.1
3.8
1.8
1.6
1.5

H1* + N54S + V56T + G109A + A174S + V206L + G182* +
1.7
1.1
1.1
1.0
3.3
1.1
1.3
1.1

D183* + K391A + G476K

H1* + N54S + V56T + Q169E + Q172K + A174* + G182* +
1.7
1.4
1.3
1.0
3.6
1.4
1.8
1.2

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.7
1.7
1.2
1.0
1.7
1.7
1.6
1.3

N195F + V206F + K391A + G476K

H1* + N54S + V56T + G109A + G182* + D183* + N195F +
1.7
1.4
1.1
1.0
1.9
1.6
1.2
1.0

V206L + K391A + G476K

H1* + N54S + V56T + G109A + Q172K + A174S + G182* +
1.7
1.5
1.1
1.1
3.6
1.6
1.5
1.3

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + Q172K + A174* + G182* +
1.6
1.4
1.1
1.1
1.6
1.4
1.3
1.1

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174N + G182* + D183* +
1.6
1.8
1.2
1.2
4.6
2.0
2.0
1.7

N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + A186N + G182* +
1.6
1.8
1.1
1.0
1.5
1.9
1.7
1.3

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.6
1.5
1.0
1.0
1.7
1.7
1.4
1.2

N195F + V206L + F289L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.6
1.2
1.0
0.8
1.2
1.2
1.3
1.1

N195F + V206L + Y295F + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.6
1.2
1.1
1.0
1.2
1.0
1.2
1.0

N195F + V206L + V291A + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.5
1.4
1.1
1.0
2.1
1.5
1.3
1.1

N195F + V206L + S244Q + K391A + G476K

G182* + D183* + F328L
1.5
1.5
1.1
1.1
1.8
1.8
1.6
1.3

G182* + D183* + N195F + A288V
1.5
1.3
1.2
1.0
1.8
1.6
1.5
1.1

G182* + D183* + A186N + N195F
1.5
1.6
0.9
0.9
2.4
1.7
1.5
1.2

G182* + D183* + E190P + N195F
1.5
1.4
0.9
1.0
2.1
1.7
1.1
1.1

W140Y + G182* + D183* + N195F
1.5
1.4
1.1
1.1
1.4
1.7
1.5
1.3

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.5
1.0
1.1
0.9
1.5
1.0
1.5
1.0

N195F + V206L + A225V + K391A + G476K

G182* + D183* + A186D + N195F
1.5
1.7
1.4
1.1
3.3
2.0
1.8
1.6

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.5
1.2
1.1
1.0
1.5
1.2
1.3
1.2

N195F + V206L + K391A + P473R + G476K

H1* + W48Y + N54S + V56T + G109A + A174S + G182* +
1.5
1.3
1.0
1.1
1.2
1.4
1.3
1.2

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + Q169E + Q172K + A174* +
1.5
1.3
1.4
1.1
3.8
1.4
1.5
1.3

G182* + D183* + N195F + V206L + K391A + G476K

G182* + D183* + N195F + V238A
1.5
1.5
1.0
1.1
1.9
1.4
1.1
1.0

G182* + D183* + Y295N
1.4
1.1
1.3
1.0
2.5
1.2
1.6
1.2

Q172G + G182* + D183*
1.4
1.4
1.1
1.0
1.4
1.3
1.5
1.3

F113Q + G182* + D183* + N195F
1.4
0.8
1.0
0.8
1.0
1.0
1.3
1.0

H1* + N54S + V56T + Q98A + G109A + A174S + G182* +
1.4
1.3
1.2
1.0
2.7
1.1
1.5
1.2

D183* + N195F + V206L + K391A + G476K

G182* + D183* + N195F + Y295F
1.3
1.3
1.2
1.1
1.3
1.2
1.4
1.1

G182* + D183* + A186E
1.3
1.2
1.2
1.0
2.0
1.4
1.4
1.2

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.3
1.1
1.2
1.1
2.8
1.2
1.3
1.2

N195F + V206Y + K391A + G476K

G182* + D183* + N195F + W284H
1.3
1.6
1.1
1.2
3.3
1.8
1.6
1.6

G182* + D183* + A186Q + N195F
1.3
1.2
1.1
1.0
2.0
1.7
1.5
1.1

K72R + G182* + D183*
1.3
1.3
1.0
1.0
1.4
1.5
1.3
1.1

D183* + G182* + N195F + V326L
1.3
1.6
1.2
1.2
3.5
1.8
1.5
1.6

D183* + G182* + A186E + N195F
1.3
1.3
1.1
1.0
1.7
1.4
1.5
1.2

Q169E + G182* + D183* + N195F
1.2
1.0
1.1
1.0
1.7
0.8
1.4
1.2

D183* + G182* + N195F + K242Q
1.2
0.8
0.9
0.9
2.0
1.2
0.9
1.0

G7A + G182* + D183* + N195F
1.2
1.2
1.1
1.0
1.5
1.6
1.4
1.1

D183* + G182* + N195F + N260G
1.2
1.4
1.0
0.9
1.6
1.2
1.3
1.1

D183* + G182* + R321A + S323N
1.2
1.3
1.1
1.1
1.2
1.1
1.5
1.2

D183* + G182* + W284H
1.2
0.9
1.2
1.0
0.9
1.0
1.1
1.1

H1* + D183* + G182*
1.2
1.5
1.0
1.0
3.6
1.7
1.6
1.3

D183* + G182* + A186H + N195F
1.1
1.3
1.1
1.0
2.0
1.2
1.4
1.0

D183* + G182* + F289V
1.1
1.3
1.0
1.0
2.2
1.3
1.7
1.4

D183* + G182* + K391A
1.1
0.7
1.0
0.8
0.7
0.7
1.0
0.8

D183* + G182* + S304R
1.1
1.2
0.9
0.9
0.6
0.9
1.0
1.0

W167F + G182* + D183* + N195F
1.1
1.0
1.0
0.9
3.3
1.1
1.3
1.1

F113Q + R116H + D183* + G182*
1.1
0.7
1.0
0.8
1.2
0.8
1.3
0.9

D183* + G182* + N270G
1.1
1.0
0.9
0.9
1.0
1.3
1.0
1.0

D183* + G182* + S244Q
1.0
1.1
1.1
0.9
1.8
1.6
1.5
1.0

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.0
1.0
1.1
1.0
0.6
0.6
0.9
0.9

G184T + N195F + V206L + K391A + G476K

D183* + G182* + N195F + Y295N
1.0
1.1
1.0
1.0
1.6
0.9
1.2
1.3

W48F + D183* + G182*
1.0
0.9
1.0
0.9
1.8
1.0
1.3
1.0

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.0
1.2
1.0
1.0
1.8
1.1
1.2
1.1

N195F + V206L + G255A + K391A + G476K

D183* + G182* + A186Q
1.0
0.7
1.1
0.9
0.4
0.5
1.0
0.9

W140Y + D183* + G182*
0.9
0.6
0.9
0.9
0.5
0.9
1.1
1.0

D183* + G182* + K242Q
0.9
0.8
1.0
0.9
0.8
0.9
1.0
1.1

D183* + G182* + V206L
0.9
0.8
0.9
0.9
1.1
0.9
0.9
0.9

D183* + G182* + N195F + V206F
0.9
0.9
0.9
1.1
0.5
0.8
1.1
1.0

Q169E + D183* + G182*
0.9
0.8
1.0
0.9
1.1
0.8
0.9
0.9

D183* + G182* + N195F + R320A
0.8
0.9
1.1
1.0
0.8
0.9
1.0
1.0

G182* + D183* + G184T + N195F
0.8
1.1
0.9
1.0
0.9
1.0
1.2
1.0

D183* + G182* + G184T
0.8
0.9
1.0
0.9
0.7
0.8
1.0
1.1

N54S + V56T + D183* + G182*
0.8
0.8
1.1
1.0
0.9
0.7
1.1
0.9

D183* + G182* + P473R
0.8
0.9
1.0
1.0
1.1
0.8
1.0
1.0

W48Y + D183* + G182*
0.8
0.9
1.1
1.1
1.0
0.6
1.0
0.9

D183* + G182* + R323A
0.8
0.8
0.9
1.0
1.1
0.8
1.1
0.8

G7A + D183* + G182*
0.8
0.8
1.1
0.9
1.3
1.1
1.2
1.0

G109A + D183* + G182*
0.8
0.8
0.8
0.8
0.0
0.6
1.0
1.1

W167F + D183* + G182*
0.7
0.7
1.0
0.9
1.4
0.9
1.1
1.0

H1* + N54S + V56T + G109A + Q169E + Q172K + A174S +
0.7
0.7
0.8
0.9
1.1
0.5
0.8
0.8

G182* + D183* + N195F + V206L + K391A + G476K

A174S + D183* + G182*
0.7
1.2
0.8
0.9
2.2
0.9
1.1
1.3

R116Q + D183* + G182*
0.7
0.7
0.9
0.9
0.7
0.6
1.1
0.9

D183* + G182* + N195F + V291A
0.7
0.9
1.0
1.1
0.6
0.9
0.9
1.0

Q172K + D183* + G182*
0.7
0.9
0.9
1.0
0.4
0.5
1.1
0.9

N54S + D183* + G182*
0.7
1.2
1.0
1.1
1.7
1.1
1.0
1.3

D183* + G182* + N195F + S323N
0.7
0.9
0.8
0.9
1.8
1.0
1.0
1.0

D183* + G182* + N195F + M246V
0.7
0.8
1.1
1.0
0.6
0.5
0.9
0.9

W48Y + D183* + G182* + N195F
0.6
1.2
1.1
1.1
2.0
1.5
1.2
1.4

D183* + G182* + N195F + S304R
0.6
1.1
0.7
1.0
1.6
0.7
1.0
1.2

V56T + D183* + G182*
0.3
0.8
0.2
0.6
1.9
0.6
0.3
0.7

A-15-
A-15-
A-40-
A-40-
J-15-
J-15-
J-30-
J-30-

0.05
0.2
0.05
0.2
0.05
0.2
0.05
0.2

Modifications in SEQ ID NO: 14
mg/L
mg/L
mg/L
mg/L
mg/L
mg/L
mg/L
mg/L

Reference
1.0
1.0
1.0
1.0
1.0
1.0
1.0
1.0

G182* + D183* + N195F
1.4
1.2
1.0
1.0
1.2
1.3
1.1
1.1

H1* + G182* + D183* + N195F
1.1
1.0
1.0
1.1
1.1
1.0
1.1
1.1

N54S + G182* + D183* + N195F
1.2
1.1
0.9
1.1
1.3
1.1
1.1
1.1

V56T + G182* + D183* + N195F
1.4
1.1
1.0
1.0
1.5
1.2
1.1
1.1

G182* + D183* + N195F + I405L
1.1
1.1
1.0
1.0
1.1
1.1
1.0
1.0

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.0
1.1
0.9
0.9
1.4
1.0
1.1
0.9

N195F + V206L + I405L + A421H + A422P + A428T +

D476K

A174S + G182* + D183* + N195F
1.2
1.0
1.0
0.9
0.9
0.9
1.0
1.0

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.4
1.3
1.1
1.0
1.6
1.3
1.3
1.1

N195F + V206L + I405L + A421H + A422P + A428T

H1* + N54S + V56T + G109A + A174S + D183* + N195F +
1.2
1.3
1.0
1.0
1.6
1.3
1.2
1.1

V206L + K391A + I405L + A421H + A422P + A428T

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.3
1.3
1.1
1.0
1.4
1.3
1.1
1.0

N195F + V206L + K391A + I405L + A421H + A422P +

A428T + D476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.2
1.1
1.1
1.0
1.4
1.2
1.2
1.1

N195F + V206L + K391A + I405L + A421H + A422P +

A428T + D476G

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.3
1.3
1.0
1.0
1.5
1.2
1.1
1.0

N195F + V206L + K391A + I405L + A421H + A422P +

A428T + D476K + G477S

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.3
1.2
1.0
1.0
1.5
1.2
1.2
1. text missing or illegible when filed

N195F + V206L + K391A + I405L + A421H + A422P +

A428T + D476K

H1* + N54S + V56T + K72R + G109A + W167F + A174S +
1.1
1.2
1.0
1.1
1.4
1.4
1.3
1. text missing or illegible when filed

G182* + D183* + N195F + V206L + I405L + A421H +

A422P + A428T

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
0.9
1.2
1.1
0.9
1.3
1.2
1.3
1. text missing or illegible when filed

N195F + V206L + S280Q + H321Y + I405L + A421H +

A422P + A428T

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.1
1.2
1.0
1.0
1.2
1.1
1.1
1.0

N195F + V206L + S304N + I405L + A421H + A422P +

A428T + D476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.2
1.2
1.0
1.0
1.2
1.0
1.0
0. text missing or illegible when filed

N195F + V206L + K391A + I405L + A421H + A422P +

A428T + D476K + G477A

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.6
1.4
1.1
1.1
1.9
1.4
1.3
1. text missing or illegible when filed

N195F + V206L + K391A + I405L + A421H + A422P +

A428T + D476G + G477T

H1* + N54S + V56T + G109A + F113Q + R116Q + Q172N +
0.9
1.2
0.8
1.1
0.8
1.7
0.9
1. text missing or illegible when filed

A174S + G182* + D183* + N195F + V206L + I405L +

A421H + A422P + A428T

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.7
1.3
1.1
1.0
1.9
1.4
1.3
1.1

N195F + V206L + K391A + I405L + A421H + A422P +

A428T + G477Q

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.5
1.4
1.1
1.0
1.9
1.4
1.3
1.1

N195F + V206L + K391A + I405L + A421H + A422P +

A428T + G477A

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.3
1.3
1.0
1.0
1.7
1.2
1.1
1.0

N195F + V206L + K391A + I405L + A421H + A422P +

A428T + D476K + G477Q

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.5
1.3
1.1
1.1
1.7
1.4
1.3
1.2

N195F + V206L + K391A + I405L + A421H + A422P +

A428T + D476N

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.5
1.3
1.1
1.0
1.7
1.3
1.3
1.2

N195F + V206L + K391A + I405L + A421H + A422P +

A428T + D476Y

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.5
1.2
1.1
1.1
2.2
1.4
1.4
1.1

N195F + V206I + I405L + A421H + A422P + A428T

H1* + N54S + V56T + G109A + Q172D + A174S + G182* +
1.4
1.3
1.1
1.0
2.8
2.0
1.4
1.2

D183* + N195F + A204G + V206L + I405L + A421H +

A422P + A428T

H1* + N54S + V56T + K72R + G109A + T134E + A174S +
1.3
1.1
1.0
1.0
2.1
2.1
1.3
1.0

G182* + D183* + N195F + A204G + V206L + G255A +

I405L + A421H + A422P + A428T

H1* + N54S + V56T + G109A + F113Q + R116H + T165G +
1.3
1.3
1.0
1.0
2.9
1.9
1.3
1.1

Q172G + A174S + G182* + D183* + N195F + V206L +

I405L + A421H + A422P + A428T

H1* + N54S + V56T + G109A + F113Q + Q172N + A174S +
1.3
1.3
1.1
1.1
1.9
2.2
1.5
1.3

G182* + D183* + N195F + V206L + I405L + A421H +

A422P + A428T

H1* + N54S + V56T + K72R + G109A + T134E + A174S +
1.4
1.3
1.1
1.1
2.5
2.3
1.5
1. text missing or illegible when filed

G182* + D183* + N195F + G196R + V206L + G255A +

I405L + A421H + A422P + A428T + N475S

H1* + N54S + V56T + G109A + T134E + A174S + G182* +
1.8
1.5
1.2
1.0
2.3
1.8
1.5
1. text missing or illegible when filed

D183* + N195F + V206L + I405L + A421H + A422P +

A428T

H1* + T40D + N54S + V56T + G109A + A174S + G182* +
2.0
1.5
1.2
1.1
1.8
1.6
1.5
1. text missing or illegible when filed

D183* + N195F + V206L + I405L + A421H + A422P +

A428T

H1* + N54S + V56T + G109A + F113Q + N125D + A174S +
2.1
1.6
1.3
1.0
2.2
1.7
1.5
1.2

G182* + D183* + N195F + V206L + I405L + A421H +

A422P + A428T

H1* + N54S + V56T + A60V + G109A + R116Q + A174S +
1.6
1.6
1.1
1.0
1.9
1.6
1.4
1. text missing or illegible when filed

G182* + D183* + N195F + V206L + I405L + A421H +

A422P + A428T

H1* + N54S + V56T + G109A + R116V + A174S + G182* +
1.4
1.4
1.0
1.0
2.1
1.4
1.4
1. text missing or illegible when filed

D183* + N195F + V206L + I405L + A421H + A422P +

A428T

H1* + N54S + V56T + G109A + R116Q + G133Q + T165G +
1.1
1.4
1.0
1.1
2.0
2.3
1.5
1. text missing or illegible when filed

Q172G + A174S + G182* + D183* + N195F + V206L +

I405L + A421H + A422P + A428T

H1* + N54S + V56T + G109A + F113Q + R116H + Q172G +
1.7
1.6
1.2
1.1
3.0
1.9
1.5
1.2

A174S + G182* + D183* + N195F + V206L + I405L +

A421H + A422P + A428T

H1* + N54S + V56T + G109A + F113Q + R116Q + Q172G +
1.4
1.5
1.1
1.1
2.1
1.8
1.3
1.2

A174S + G182* + D183* + N195F + V206L + I405L +

A421H + A422P + A428T + G448D

H1* + N54S + V56T + G109A + F113Q + R116H + Q172N +
2.2
1.6
1.3
1.2
2.5
1.6
1.6
1.3

A174S + G182* + D183* + N195F + V206L + I405L +

A421H + A422P + A428T

H1* + N54S + V56T + K72R + G109A + T134E + W167F +
2.2
1.6
1.3
1.2
2.4
1.6
1.7
1.2

Q172N + A174S + G182* + D183* + N195F + V206L +

G255A + A265G + I405L + A421H + A422P + A428T

H1* + N54S + V56T + K72R + G109A + R116Q + W167F +
2.0
1.6
1.2
1.2
2.3
1.6
1.7
1.2

Q172R + L173V + A174S + G182* + D183* + N195F +

V206L + A265G + I405L + A421H + A422P + A428T

H1* + N54S + V56T + K72R + G109A + R116Q + W167F +
2.1
1.4
1.3
1.2
2.3
1.6
1.7
1.2

Q172R + A174S + G182* + D183* + N195F + V206L +

I405L + A421H + A422P + A428T

H1* + N54S + V56T + K72R + G109A + F113Q + T134E +
2.0
1.5
1.1
1.1
2.6
1.9
1.4
1.2

W167F + Q172G + A174S + G182* + D183* + N195F +

V206L + G255A + I405L + A421H + A422P + A428T

H1* + N54S + V56T + K72R + G109A + F113Q + R116H +
1.7
1.6
1.0
0.9
1.8
1.5
1.1
1.1

W167F + Q172G + L173V + A174S + G182* + D183* +

G184T + N195F + V206L + I405L + A421H + A422P + A428T

H1* + N54S + V56T + G109A + F113Q + W167F + Q172G +
1.2
1.2
1.0
0.9
2.2
1.3
1.2
1.1

A174S + G182* + D183* + N195F + V206L + I405L +

A421H + A422P + A428T

H1* + N54S + V56T + K72R + G109A + A174S + G182* +
1.5
1.2
1.2
1.0
2.2
1.1
1.5
1. text missing or illegible when filed

D183* + N195F + V206L + G255A + I405L + A421H +

A422P + A428T

H1* + N54S + V56T + G109A + F113Q + R116Q + A174S +
1.6
1.3
1.1
1.0
1.4
1.2
1.3
1. text missing or illegible when filed

G182* + D183* + N195F + V206L + I405L + A421H +

A422P + A428T

H1* + N54S + V56T + K72R + G109A + F113Q + F116H +
1.8
1.6
1.0
1.0
0.9
1.4
0.9
1. text missing or illegible when filed

W167F + Q172G + L173V + A174S + G182* + D183* +

N195F + V206L + I405L + A421H + A422P + A428T

H1* + N54S + V56T + K72R + G109A + F113Q + R116Q +
1.8
1.5
1.1
1.0
1.9
1.5
1.3
1. text missing or illegible when filed

W167F + Q172G + A174S + G182* + D183* + G184T +

N195F + V206L + I405L + A421H + A422P + A428T

H1* + N54S + V56T + G109A + F113Q + R116Q + W167F +
2.0
1.5
1.0
1.0
1.8
1.5
1.2
1.0

Q172G + A174S + G182* + D183* + N195F + A204G +

V206L + I405L + A421H + A422P + A428T

H1* + N54S + V56T + G109A + F113Q + R116H + W167F +
1.5
1.3
0.9
0.9
1.4
1.3
1.2
1. text missing or illegible when filed

Q172G + A174S + G182* + D183* + N195F + V206L +

A265G + I405L + A421H + A422P + A428T

H1* + N54S + V56T + G109A + R116Q + A174S + G182* +
1.8
1.6
1.0
0.9
1.6
1.3
1.4
1. text missing or illegible when filed

D183* + N195F + V206L + I405L + A421H + A422P +

A428T

H1* + N54S + V56T + A60V + G109A + R116Q + W167F +
1.4
1.4
0.7
0.9
1.1
1.3
0.8
1. text missing or illegible when filed

Q172N + L173V + A174S + G182* + D183* + N195F +

V206L + I405L + A421H + A422P + A428T

H1* + A37H + N54S + V56T + A60V + G109A + R116Q +
1.9
1.6
1.1
1.1
1.7
1.6
1.4
1.2

T165G + A174S + G182* + D183* + N195F + V206L +

I405L + A421H + A422P + A428T

H1* + N54S + V56T + G109A + A174S + M105F + G182* +
1.4
1.2
1.0
1.0
1.5
1.2
1.2
1.0

D183* + N195F + V206L + L228I + R320A + S323N +

I405L + A421H + A422P + A428T

H1* + N54S + V56T + K72R + G109A + F113Q + T134E +
1.8
1.5
1.1
1.0
1.7
2.1
1.4
1.2

A174S + G182* + D183* + N195F + V206L + G255A +

I405L + A421H + A422P + A428T

H1* + N54S + V56T + K72R + G109A + R116Q + V120L +
2.1
1.8
1.2
1.1
2.8
2.5
1.6
1.3

A174S + G182* + D183* + G184T + N195F + V206L +

I405L + A421H + A422P + A428T

H1* + N54S + V56T + G109A + F113Q + R116H + W167F +
2.6
1.9
1.2
1.1
2.8
2.6
1.6
1.3

Q172G + L173V + A174S + G182* + D183* + N195F +

V206L + I405L + A421H + A422P + A428T

H1* + N54S + V56T + G109A + F113Q + R116Q + Q172N +
2.3
1.6
1.1
1.1
3.0
2.1
1.5
1.3

A174S + G182* + D183* + N195F + V206L + A265G +

I405L + A421H + A422P + A428T

H1* + A37H + N54S + V56T + A60V + G109A + R116Q +
2.1
1.7
1.2
1.1
2.5
2.1
1.5
1.2

W167F + Q172R + A174S + G182* + D183* + N195F +

V206L + I405L + A421H + A422P + A428T

text missing or illegible when filed

indicates data missing or illegible when filed

Example 4
Wash Performance of a Subset of Variants According to the Invention (AMSA)

The following variants have been tested as described above. The color measurements were done by use of a professional flatbed scanner (Kodak iQsmart, Kodak) used to capture an image of the washed textile and with a controlled digital imaging system (DigiEye) for capture an image of the washed melamine plates.

Int=√{square root over (r²+g²+b²)}

The results obtained were the following;

A-15-
A-15-
A-40-
A-40-
J-15-
J-15-
J-30-
J-30-

0.05
0.2
0.05
0.2
0.05
0.2
0.05
0.2

Modifications in SEQ ID NO: 13
mg/L
mg/L
mg/L
mg/L
mg/L
mg/L
mg/L
mg/L

Reference
1.0
1.0
1.0
1.0
1.0
1.0
1.0
1.0

G182* + D183* + N195F + V206F + N260P + L312I +
0.7
1.0
0.6
0.7
1.3
0.9
0.8
1.0

L313I + R320A + T355L + L389I + T400P

G182* + D183* + N195F
0.1
0.7
1.0
1.0
1.0
0.8
1.0
1.0

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
1.7
1.9
1.7
1.3
2.5
1.9
1.2
1.2

N195F + V206L + K391A + G476K

H1* + G50A + T51A + V56T + G109A + A174S + G182* +
1.5
1.6
1.5
1.4
2.5
1.6
1.3
1.2

D183* + N195F + V206L + G476K

H1* + N54S + V56T + M105F + G109A + A174S + G182* +
2.5
2.2
1.6
1.4
3.8
1.9
1.2
1.1

D183* + N195F + V206L + R320A + S323N + K391A + G476K

H1* + T40K + N54S + V56T + G109A + G149H + A174S +
0.9
1.2
1.0
1.0
0.8
1.2
0.9
1.0

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + K72R + G109A + A174S + G182* +
1.9
1.7
1.4
1.4
2.8
1.5
1.2
1.1

D183* + N195F + V206L + G255A + K391A + G476K

H1* + N16H + V17L + N54S + V56T + G109A + A174S +
2.6
1.8
1.7
1.4
3.6
1.8
1.3
1.1

G182* + D183* + N195F + V206L + K391A + G476K

H1* + Q22N + N54S + V56T + G109A + A174S + G182* +
2.5
1.9
1.8
1.4
3.8
2.1
1.3
1.1

D183* + N195F + V206L + K391A + G476K

H1* + Q32S + N54S + V56T + G109A + A174S + G182* +
2.3
1.9
1.9
1.5
3.3
2.0
1.2
1.2

D183* + N195F + V206L + K391A + G476K

H1* + H28N + N54S + V56T + G109A + A174S + G182* +
2.7
1.7
1.6
1.5
4.2
2.0
1.3
1.1

D183* + N195F + V206L + K391A + G476K

H1* + N29S + N54S + V56T + G109A + A174S + G182* +
1.6
1.3
1.3
1.4
2.6
1.6
1.2
1.0

D183* + N195F + V206L + K391A + G476K

H1* + H28N + N29S + N54S + V56T + G109A + A174S +
1.8
1.9
1.8
1.4
3.1
1.8
1.2
1.1

G182* + D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + R176K +
2.3
2.0
1.7
1.5
5.1
1.9
1.3
1.2

N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + Q169E + Q172K + A174* +
3.8
2.4
2.1
1.7
5.6
2.8
1.4
1.2

R176K + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + N175G +
2.6
1.8
1.8
1.4
4.9
2.0
1.3
1.2

N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + N195F + V206L +
1.7
1.4
1.4
1.3
2.7
1.6
1.0
1.1

V291A + Y295N + Q299T + K391A + G476K

H1* + N54S + V56T + G109A + A174S + N195F + V206L +
1.7
1.4
1.6
1.2
1.4
1.8
1.0
1.0

K269S + A274K + K391A + G476K

H1* + N54S + V56T + G109A + A174S + N195F + V206L +
2.6
1.5
2.0
1.3
1.3
2.1
1.0
1.1

L235I + K391A + G476K

H1* + N54S + V56T + G109A + A174S + N195F + V206L +
2.8
1.4
1.7
1.2
0.6
2.2
1.1
1.0

L235V + V238A + K391A + G476K

H1* + N54S + V56T + G109A + A174S + N195F + V206L +
1.9
1.5
1.9
1.3
2.0
1.7
1.2
1.0

Q365S + K391A + G476K

H1* + N54S + V56T + G109A + A174S + N195F + V206L +
2.2
1.4
2.0
1.2
0.6
1.2
1.1
1.1

K391A + H402R + G476K

H1* + N54S + V56T + G109A + A174S + N195F + V206L +
5.6
2.7
2.5
1.6
4.2
2.9
1.2
1.2

R320S + H321N + K391A + G476K

H1* + N54S + V56T + G109A + Q169E + Q172K + A174* +
3.8
2.6
3.0
1.7
3.6
2.8
1.3
1.2

N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + Q125S + A174S + N195F +
3.1
2.2
2.4
1.4
2.0
2.3
1.3
1.1

V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + N195F + V206L +
4.2
1.9
1.9
1.5
1.8
2.4
1.0
1.0

L235V + K391A + G476K

H1* + N54S + V56T + G109A + A174S + N195F + V206L +
4.2
1.5
2.2
1.4
1.2
2.6
1.1
1.1

A265G + K391A + G476K

H1* + T51Q + N54S + V56T + G109A + A174S + N195F +
3.7
2.1
2.6
1.6
3.6
2.7
1.3
1.1

Y203F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + Q169E + Q172K + A174* +
4.0
2.0
2.7
1.5
3.3
2.8
1.2
1.2

N175Q + N195F + V206L + K391A + G476K

H1* + H28N + N54S + V56T + G109A + A174S + G182* +
4.5
2.3
2.5
1.6
3.1
2.7
1.2
1.1

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + T134E + A174S + G182* +
3.9
2.1
2.6
1.6
3.3
3.0
1.4
1.1

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
5.3
2.9
3.0
1.7
3.8
2.9
1.3
1.2

N195F + V206L + D377H + K391A + G476K

H1* + G50A + T51A + N54S + V56T + G109A + A174S +
4.4
2.1
2.3
1.4
2.6
2.3
1.3
1.2

G182* + D183* + N195F + V206L + K391A + G476K

H1* + G7K + N54S + V56T + G109A + A174S + G182* +
4.1
2.3
2.7
1.6
2.4
2.5
1.3
1.2

D183* + N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + G182* + D183* +
2.7
1.9
1.4
1.4
1.9
2.2
0.9
1.0

N195F + M202V + V206L + K391A + G476K

H1* + N54S + V56T + M105F + G109A + A174S + N195F +
3.7
2.1
2.4
1.5
2.7
2.5
1.2
1.1

V206L + M246F + K391A + G476K

H1* + N16H + V17L + N54S + V56T + G109A + A174S +
4.5
2.7
1.8
1.4
4.3
2.2
1.5
1.3

G182* + D183* + N195F + V206L + D377H + K391A + G476K

H1* + N54S + V56T + G109A + A174S + N195F + V206L
1.1
1.9
1.3
1.1
3.0
1.9
1.3
1.1

H1* + T51G + N54S + V56T + G109A + A174S + N195F +
2.2
1.8
1.1
1.0
2.7
2.0
0.9
1.1

Y203G + V206L + K391A + G476K

H1* + T51G + N54S + V56T + G109A + G110N + A174S +
1.6
2.1
1.0
1.1
1.9
1.9
1.1
1.1

N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + Q125S + A174S + N175G +
2.0
2.3
1.5
1.2
2.6
1.8
1.3
1.2

N195F + V206L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + N195F +
0.7
2.1
1.1
1.0
1.8
1.7
1.0
1.1

V206L + A265G + V291A + F328L + K391A + G476K

H1* + N54S + V56T + G109A + A174S + N195F + V206L +
3.9
2.6
1.9
1.4
4.0
2.1
1.5
1.2

M246F + A265G + V291A + F328L + Q365S + K391A + G476K

H1* + N16Y + N54S + V56T + G109A + R116H + A174S +
4.8
3.5
2.0
1.5
4.9
2.7
1.5
1.3

N195F + V206L + G337E + D377H + K391A + G476K

H1* + I9L + N54S + V56T + G109A + Q125S + V131T +
3.1
2.9
1.4
1.2
2.5
2.0
1.2
1.1

A174* + V206L + Y267F + F289H + Q361E + K391A + G476K

The invention described and claimed herein is not to be limited in scope by the specific aspects herein disclosed, since these aspects are intended as illustrations of several aspects of the invention. Any equivalent aspects are intended to be within the scope of this invention. Indeed, various modifications of the invention in addition to those shown and described herein will become apparent to those skilled in the art from the foregoing description. Such modifications are also intended to fall within the scope of the appended claims. In the case of conflict, the present disclosure including definitions will control.

Claims

1. A variant of a parent polypeptide having alpha-amylase activity, wherein said variant is a fusion polypeptide which has an improved wash performance at low temperature, and wherein said variant has alpha-amylase activity.
2. The variant according to claim 1, wherein said low temperature is 5° C. to 40° C., preferably 10° C. to 30° C., even more preferred 15° C. to 20° C.
3. The variant according to claim 1, wherein said improved wash performance has an Improvement Factor (IF) of ≥1 when compared to said parent polypeptide having alpha-amylase activity.
4. The variant according to claim 1, wherein said parent polypeptide comprises an A and a B domain comprising the amino acid sequence of SEQ ID NO: 10, or an A and a B domain having at least 80%, such as 85%, such as 90%, such as 95%, such as 97% sequence identity to SEQ ID NO: 10, and a C domain of a low temperature alpha-amylase.
5. The variant according to claim 1, wherein said parent polypeptide comprises a C domain comprising the amino acid sequence of SEQ ID NOs: 11 or 12, or a C domain having at 80%, at least 85%, at least 90%, at least 95%, at least 97%, sequence identity to SEQ ID NO: 11 or 12.
6. The variant according to claim 1, wherein said parent polypeptide comprises the amino acid sequence of SEQ ID NOs: 13 or 14, or a sequence having at least 80%, at least 85%, at least 90%, at least 95%, or at least 97% sequence identity to SEQ ID NOs: 13 or 14.
7. The variant according to claim 1, wherein said variant comprises an amino acid sequence having at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least 99%, but less than 100% sequence identity to any one of the SEQ ID NOs: 4, 7, 13, 14, or 15.
8. The variant according to claim 1, wherein said A and B domains comprise a modification in one or more of the following positions corresponding to positions; H1, T5, G7, Q11, N16, V17, Q32, A37, T40, P45, W48, G50, T51, N54, V56, A60, K72, R87, Q98, M105, G109, F113, R116, Q118, Q125, G133, T134, W140, G142, G149, T165, W167, Q169, R171, Q172, L173, A174, G184, A186, E190, T193, N195, G196, A204, V206, P211, I214, V215, L217, L219, A225, L228, L235, V238, K242, S244, M246, M248, L250, G255, Q256, N260, A263, V264, A265, Y267, K269, N270, G273, S280, W284, T285, M286, F289, V291, Y295, Q299, S304, R320, H321, S323, H324, V326, F328, T334, D337, Q345, G346, G348, T355, S376, D377, D379, Y382, S383, Q385, K391, K393, and Q395 of SEQ ID NOs: 7 or 10.
9. The variant according to claim 7, wherein said modifications in one or more positions correspond to the following; H1*, TSK, G7K, G7A, Q11H, N165, N16H, V17L, Q32S, A37H, A37M, A37V, A37S, A37Y, A37R, A37L, T40D, T40G, T40K, P45A, W48G, W48Y, W48F, G50A, T51K, T51E, T51G, T51A, T51S, T51G, T51D, N54S, V56T, A60V,K72R, K72H, K725, K72Q, K72E, K72N, K72A, K72M, R87S, Q98S, Q98A, M105F, M105I, M105V, M105L, G109A, G109S, F113W, F113S, F113N, F113Y, F113R, F113L, F113Q, R116Q, R116V, R116K, R116W, R116L, R116A, R116H, R116M, R116E, R116S, R116I, R116G, Q118N, Q118K, Q118G, Q118S, Q118F, Q118R, Q125P, Q125K, Q125A, Q125T, N125D, G133S, F133Q, T134E, W140Y, G142T, G149Q, T165S, T165G, T165V, W167I, W167G, W167F, W167R, W167S, W167H, W167L, W167M, W167Y, Q169E, R171H, Q172G, Q172R, Q172N, Q172D, Q172Y, Q172M, Q172S, Q172T, Q172K, Q172H, Q172E, L173V, L173G, L173H, L173A, L173I, L173P, L173T, L173F, L173M, A174S, A174D, A174P, A174M, A174T, A174H, A174K, A174G, A174Q, A174N, A174V, A174L, G184T, A186D, A186N, A186E, A186Q, A186H, E190P, T193K, N195F, G196R, A204T, A204V, A204S, A204G, V206L, V206S, V206Y, P211D, I214G, I214H, I214S, I214T, I214L, I214E, I214W, V215T, L217T, L217Q, L219V, L219H, A225V, L228I, L235V, L235A, V238A, V238T, V238G, K242Q, S244Q, M246L, M246A, M246I, M246F, M246V, M246S, M248T, L250I, L250V, L250T, L250A, L250F, L250M, G255N, G255A, G255S, Q256A, N260G, A263G, V264I, V264T, A265G, Y267I, Y267M, Y267H, Y267L, K269Q, N270G, N270T, G273R, S280W, S280L, S280T, S280A, S280K, S280Q, W284H, T285L, T285Q, M286F, M286L, A288L, A288V, F2891, F289L, V291G, V291A, V291T, Y295N, Y295F, Q299V, S304N, S304R, R320A, R320V, H321Y, S323N, H324L, V326L, F328L, F328M, F328V, F328I, T334S, D337H, Q345D, G346T, G346D, G346P, G348S, G348P, T355L, T355F, S376H, S376T, S376V, D377H, D377S, D377A, D377Q, D379S, D379G, D379R, D379A, Y382M, Y382I, Y382L, Y382F, S383G, S383A, Q385L, K391A, K391Y, K391V, K391M, K391E, K391D, K391R, K391H, K391W, K391I, K391Q, K391L, K393Y, K393R, K393Q, K393S, and Q395P, wherein said positions correspond to positions of SEQ ID NOs: 7 or 10.
10. The variant according to claim 1, wherein said C domain comprises an alteration in one or more of the following positions corresponding to positions; A420, G423, T444, A445, Q449, T459, P473, C474, G476, G477, and K484 of SEQ ID NO: 13 or I405, A421, A422, A428, R439, G448, W467, D476, and G477 of SEQ ID NO: 14.
11. The variant according to claim 9, wherein said alteration in one or more positions correspond to the following T400P, H402R, A420Q, A420S, A420K, A420L, G423H, T444Q, T444S, T444D, T444Y, T444H, T444V, T444R, T444A, A445Q, Q449T, T459N, P473R, P473A, P473G, P473T, P473K, C474V, G476K, G477A, G477Q, K484A, K484G, K484P, K484E, K484Q, and K484S, wherein said positions correspond to positions of SEQ ID NO: 13; or I405L, A421H, A422P, A428T, G448D, D476K, D476G, D476N, D476Y, G477S, G477A, G477T, and G477Q, wherein said positions correspond to positions of SEQ ID NO: 14.
12. The variant according to claim 1, wherein said variant comprises a) a deletion and/or a substitution at two or more positions corresponding to positions R181, G182, D183, and G184 of the amino acid sequence as set forth in SEQ ID NOs: 7 or 10, andb) an alteration at one or more positions corresponding to positions H1, T5, G7, Q11, N16, V17, Q32, A37, T40, P45, W48, G50, T51, N54, V56, K72, R87, Q98, M105, G109, F113, R116, Q118, Q125, G133, T134, W140, G142, G149, T165, W167, Q169, R171, Q172, L173, A174, G184, A186, E190, T193, N195, A204, V206, P211, I214, V215, L217, L219, A225, L235, V238, K242, S244, M246, M248, L250, G255, Q256, N260, A263, V264, Y267, K269, N270, G273, S280, W284, T285, M286, F289, V291, Y295, Q299, R320, H321, S323, H324, V326, F328, T334, D337, Q345, G346, G348, T355, S376, D377, D379, Y382, S383, Q385, K391, K393, Q395, A420, G423, T444, A445, Q449, T459, P473, C474, G476, G477, and K484 of SEQ ID NO: 13; or H1, A37, T40, N54, V56, A60, K72, G109, F113, R116, N125, F133, T134, T165, Q169, Q172, L173, A174, G184, A186, N195, G196, A204, V206, A225, L228, K242, S244, G255, N260, A265, K269, S280, W284, Y295, S304, R320, H321, S323, V326, K391, I405, A421, A422, A428, R439, G448, W467, D476, and G477 of SEQ ID NO: 14.
13. The variant according to claim 11, wherein the deletion a) is selected from the group consisting of R181+G182, R181+D183, R181+G184, G182+D183, G182+G184, or D183+G184.
14. The variant according to claim 11, wherein said variant comprises one or more of the following alterations; H1*, T5K, G7K, G7A, Q11H, N16S, N16H, V17L, Q32S, A37H, A37M, A37V, A37S, A37Y, A37R, A37L, T40G, T40K, P45A, W48G, W48Y, W48F, G50A, T51K, T51E, T51G, T51A, T51S, T51G, T51D, N54S, V56T, K72R, K72H, K72S, K72Q, K72E, K72N, K72A, K72M, R87S, Q98S, Q98A, M105F, M105I, M105V, M105L, G109A, G109S, F113W, F113S, F113N, F113Y, F113R, F113L, F113Q, R116Q, R116V, R116K, R116W, R116L, R116A, R116H, R116M, R116E, R116S, R116I, R116G, Q118N, Q118K, Q118G, Q118S, Q118F, Q118R, Q125P, Q125K, Q125A, Q125T, G133S, T134E, W140Y, G142T, G149Q, T165S, T165G, T165V, W167I, W167G, W167F, W167R, W167S, W167H, W167L, W167M, W167Y, Q169E, R171H, Q172G, Q172R, Q172N, Q172D, Q172Y, Q172M, Q172S, Q172T, Q172K, Q172H, Q172E, L173V, L173G, L173H, L173A, L173I, L173P, L173T, L173F, L173M, A174S, A174D, A174P, A174M, A174T, A174H, A174K, A174G, A174Q, A174N, A174V, A174L, G184T, A186D, A186N, A186E, A186Q, A186H, E190P, T193K, N195F, A204T, A204V, A204S, A204G, V206L, V206S, V206Y, P211D, I214G, I214H, I214S, I214T, I214L, I214E, I214W, V215T, L217T, L217Q, L219V, L219H, A225V, L235V, L235A, V238A, V238T, V238G, K242Q, S244Q, M246L, M246A, M2461, M246F, M246V, M246S, M248T, L250I, L250V, L250T, L250A, L250F, L250M, G255N, G255A, G255S, Q256A, N260G, A263G, V264I, V264T, Y267I, Y267M, Y267H, Y267L, K269Q, N270G, N270T, G273R, S280W, S280L, S280T, S280A, S280K, S280Q, W284H, T285L, T285Q, M286F, M286L, A288L, A288V, F2891, F289L, V291G, V291A, V291T, Y295N, Y295F, Q299V, S340R, S304N, R320A, R320V, H321Y, S323N, H324L, V326L, F328L, F328M, F328V, F3281, T334S, D337H, Q345D, G346T, G346D, G346P, G348S, G348P, T355L, T355F, S376H, S376T, S376V, D377H, D377S, D377A, D377Q, D379S, D379G, D379R, D379A, Y382M, Y382I, Y382L, Y382F, S383G, S383A, Q385L, K391A, K391Y, K391V, K391M, K391E, K391D, K391R, K391H, K391W, K391I, K391Q, K391L, K393Y, K393R, K393Q, K393S, Q395P, T400P, H402R, A420Q, A420S, A420K, A420L, G423H, T444Q, T444S, T444D, T444Y, T444H, T444V, T444R, T444A, A445Q, Q449T, T459N, P473R, P473A, P473G, P473T, P473K, C474V, G476K, G477A, G477Q, K484A, K484G, K484P, K484E, K484Q, and K484S of SEQ ID NO: 13; or H1*, G7A, A37H, T40D, W48Y, W48F, N54S, V56T, A60V, K72R, Q98A, G109A, F113Q, R116H, R116V, R116Q, N125D, F133Q, T134E, T165G, Q169E, Q172D, Q172G, Q172N, L173V, A174S, G184T, A186D, A186N, A186E, A186Q, A186H, E190P, N195F, G196R, A204G, V206L, V206Y, A225V, L228I, K242Q, S244Q, G255A, G255S, N260G, A265G, K269Q, N270T, S280Q, W284H, A288L, A288V, F289L, Y295N, Y295F, S304N, S340R, S304N, R320A, H321Y, S323N, V326L, K391A, I405L, A421H, A422P, A428T, G448D, D476K, D476G, D476N, D476Y, G477S, G477A, G477T, and G477Q of SEQ ID NO: 14.
15. The variant according to claim 1, wherein said variant has an IF of >1 when compared to said parent polypeptide, and wherein said variant is selected from G182*+D183*+N195F H1*+N54S+V56T+G109A+A1745+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+R875+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T40G+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72H+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37H+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37M+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37Y+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37R+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113W+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q118N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116W+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116L+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+G142T+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q125P+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q125K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S381G+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246T+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V213T+K391A+G476K, H1*+A37L+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q118K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q125A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377S+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377G+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377R+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377A+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214G+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S381A+K391A+G476K, H1*+N54S+V56T+G109A+W167I+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W167G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346S+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346P+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214S+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214T+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420Q+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420S+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420K+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420L+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391Y+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391R+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473G+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473T+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+Q395P+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444S+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444D+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444Y+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280W+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280T+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280A+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255N+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T285L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T285Q+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+Q449T+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473K+G476K, H1*+N54S+V56T+K72S+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T40K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346T+K391A+G476K, H1*+G50A+N54S+V56T+G109A++Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484A, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484G, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484P, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484E, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484Q, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484S, H1*K+G50A+N54S+V56T+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+L173V+A174S+G182*+D183*+N195F+A204T+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W140Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+Q98S+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+H324L+K391A+G476K, H1*+N54S+V56T+G109A+G149Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346T+K391A+G476K+G477A, H1*+N54S+V56T+G109AG255A++A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+G50A+N54S+V56T+G109A+F113L+R116L+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+G477Q, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Q345D+K391A+G476K+G477Q, H1*+G50A+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V2641+K391A+G423H+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320V+K391A+G476Y, H1*+T5K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L217V+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L217H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+P211D+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391Q+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444H+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444R+G476K, H1*+G50A+N54S+V56T+G109A+L173V+A174S+G182*+D183*+N195F+A204V+V206L+K391A+G476K, H1*+G50A+N54S+V56T+G109A+F113L+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+G7K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K, H1*+N54S+V56T+G109A+Q118G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280K+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N270G+K391A+G476K, H1*+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+G346T+K391A+G476K+G477A, H1*+N54S+V56T+G109A+Q118S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G337H+Q385L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y382M+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+K72R+M105F+G109A+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+M105F+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+M105F+G109A+Q118F+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+S323N+D377H+K391A+G476K, H1*+T51E+N54S+V56T+G109A+A174H+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+L173A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Q299V+K391A+G476K, H1*+N54S+V56T+G109A+L173G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T355L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T355F+K391A+G476K, H1*+G50A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204T+V206L+K391A+G476K, H1*+G50A+N54S+V56T+G109A+A174E+G182*+D183*+N195F+A204T+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+R320A+S323N+K391A+G476K, H1*+N54S+V56T+M105I+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246L+K391A+G476K, H1*+T51G+N54S+V56T+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51A+N54S+V56T+G109A+A174G+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174D+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51S+N54S+V56T+G109A+A174P+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51G+N54S+V56T+G109A+A174M+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72A+G109A+W167S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+W167R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+W167H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+Q11H+N54S+V56T+G109A+Q118R+T134E+A174S+G184T+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+G109A+Q118R+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+Q172D+A174Q+G182*+D183*+N195F+V206L+K391A+G476K+G477A, H1*+N54S+V56T+K72A+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W167S+G182*+D183*+N195F+A174S+V206L+K391A+G476K, H1*+N54S+V56T+K72H+G109A+W167S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+W167T+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72E+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+W167L+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+W167M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+W167H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K, H1*+N54S+V56T+G109A+Q118R+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184A+T193K+N195F+V206L+K391A+G476K, H1*+N54S+V56T+M105F+G109A+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+R320A+S323N+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291G+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+F328L+D377H+K391A+G476K, H1*+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238A+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238T+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238G+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F328M+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F328L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F328V+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F3281+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291T+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+D377H+K391A+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+G109A+L173H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q125T+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N16S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72N+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72N+G109A+W167L+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72A+G109A+W167Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+G184T+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q118R+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+R320A+S323N+K391A+G476K, H1*+N54S+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377R+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L217T+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377S+K391A+G476K, H1*+N54S+V56T+G109A+R116E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L217Q+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346D+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377A+K391A+G476K, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346P+K391A+G476K, H1*+N54S+V56T+G109A+R116S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377Q+K391A+G476K, H1*+N54S+V56T+G109A+R1161+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174F+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174T+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+T51G+N54S+V56T+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51A+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51S+N54S+V56T+G109A+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51A+N54S+V56T+G109A+Q172S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51E+N54S+V56T+G109A+Q172T+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51A+N54S+V56T+G109A+Q172A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51S+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51G+N54S+V56T+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51A+N54S+V56T+G109A+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51E+N54S+V56T+G109A+Q172L+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51E+N54S+V56T+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51D+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51A+N54S+V56T+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q172S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72H+G109A+Q172T+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q172R+A174S+K179E+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q172M+A174S+G182*+D183*+N195F+V206L+Y295N+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q172T+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72M+G109A+Q172S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72S+G109A+A174H+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+A174D+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174D+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+A174K+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174G+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174Q+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+A174G+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72S+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174H+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391S+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444A+G476K, H1*+N54S+V56T+G109A+F113L+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+G50A+N54S+V56T+G109A+A174E+G182*+D183*+N195F+A204T+V206L+S323N+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q118R+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+M105F+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q118R+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A225F+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246A+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y382I+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S376H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214E+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214W+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204T+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204S+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+T165S+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T334S+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A263G+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M286F+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y2671+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L235V+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L235A+D377H+K391A+G476K, H1*+N54S+V56T+M105L+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+M105I+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+M105V+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246I+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250I+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250V+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391Y+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391M+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391E+G476K, H1*+N54S+V56T+G109A+T165G+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54R+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54L+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174V+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246F+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246V+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246S+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174R+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174L+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206S+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391D+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391R+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391H+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391W+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391I+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250T+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250A+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250F+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250M+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391Q+G476K, H1*+N54S+V56T+G109A+Q172H+L173A+A174P+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172E+L173A+A174R+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+W167R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+G184T+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+G255A+D377H+K391A+G476K, H1*+N54S+V56T+G109A+Q118R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S376T+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S376V+K391A+G476K, H1*+N54S+V56T+G109A+Q172E+L1731+A174N+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172D+L173A+A174T+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267M+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267H+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238A+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238T+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238G+K391A+G476K, H1*+N54S+V56T+M105V+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V264T+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V264I+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L235V+K391A+G476K, H1*+N54S+V56T+G109A+W167H+Q172N+L173A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W167F+Q172E+L173P+A174K+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172N+L173T+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172K+L173V+A174T+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172E+L173F+A174R+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172E+L173T+A174R+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172N+L173V+A174R+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+L173V+A174R+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172E+L173M+A174H+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172E+L173F+A174P+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W167Y+Q172E+L173V+A174H+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+L173A+A174T+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+F328L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246S+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250V+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250F+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250M+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204S+V206L+K391A+G476K, H1*+N54S+V56T+G109A+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T334S+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A263G+M286F+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A263G+M286L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267I+K391A+G476K, H1*+T51A+N54S+V56T+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391L+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109S+F113Q+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+A204G+V206L+G255A+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+W167F+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+G184T+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+P473G+G476K, H1*+N54S+V56T+G109A+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+M105F+G109A+A174S+G182*+D183*+N195F+V206L+R320A+S323N+K391A+C474V+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+G255A+K391A+C474V+G476K, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+C474V+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A265G+K391A+T444Q+G476K+G477A, H1*+A37V+N54S+V56T+G109A+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G346T+G477A+G476K, H1*+N54S+V56T+G109A+R116Q+Q172D+A174S+G182*+D183*+N195F+V206L+G346T+K391A+T444Q+G477A+G476K, H1*+N54S+V56T+G109A+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+G346T+K391A+T444Q+G477A+G476K, H1*+N54S+V56T+G109S+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109S+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109S+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+V2641+K391A+C474V+G476K, H1*+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280Q+H321Y+K391A+C474V+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+C474V+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109A+F113Q+R116Q+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+R116H+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+T165G+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116Q+T165G+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+F289I+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116Q+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116H+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+T165G+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+Q395P+G476K, H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+A265G+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+K72R+G109A+T134E+T165G+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+A265G+K391A+P473R+G476K, H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51V+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280Q+H321Y+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109A+R116Q+W167F+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+G133Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+Q395P+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+K72R+G109A+R116H+W167F+Q172R+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+G184T+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109S+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109S+R116Q+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109S+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+G109S+F113Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+R116H+T134E+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+K72R+G109A+R116H+T134E+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+G255A+K391A+Q395P+T444Q+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+T134E+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+G255A+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+T134E+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+G255A+K391A+Q395P+A445Q+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116H+T134E+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+G255A+K391A+Q395P+G476K, H1*+N54S+V56T+K72R+G109A+T134E+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+G255A+A265G+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+K72R+G109A+G133Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172N+L173V+A174S+G182*+D183*+G184T+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+A37H+N54S+V56T+G109A+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37H+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+T459N+G476K, H1*+N54S+V56T+G109A+F113Q+Q172R+L173V+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+A60V+G109A+R116H+Q172R+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206+K391A+G476K H1*+A37H+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206+K391A+G476K H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37H+N54S+V56T+A60V+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37H+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116A+W167F+Q172R+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+R116A+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+R116A+T165G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109A+G182*+D183*+N195F+K391A+G476K, H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L, N54S+V56T+G109A+G182*+D183*+N195F, N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+G476K, H1*+N54S+V56T+A174S+G182*+D183*+N195F+V206L+G273R+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L, H1*+W48G+G109A+G182*+D183*+N195F+V206L+K391A+G476K, N54S+V56T+G109A+A174S+G182*+D183*+N195F+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+K391A, H1*+N54S+V56T+G109A+F113Q+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+R116Q+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+G109A+R116A+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+M246F+K391A+G476K, H1*+V56A+G109A+A174S+G182*+D183*+N195F+V206L+G476K, H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+K391A, H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+G476K, G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, N54S+V56T+G109A+G182*+D183*+N195F+V206L+K391T+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116H+A174S+G182*+D183*+G184T+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109S+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+F113Q+R116Q+Q172N+A174S+G182*+D183*+N195F+V206L+A265G+K391A+P473R+G476K, H1*+N54S+V56T+G109A+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+R116A+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109A+R116H+Q172R+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K, H1*+A37V+N54S+V56T+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37H+N54S+V56T+G109A+R116H+T165G+A174S+G182*+D183*+N195F+V206L+M246F+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+K391A+G476K, H1*+G109A+G182*+D183*+N195F+V206L+K391A+G476K, G109A+G182*+D183*+N195F+V206L+G476K, H1*+G109A+G182*+D183*+N195F+K391A, H1*+N54S+V56T+G109A+G133S+A174S+G182*+D183*+N195F+K391A+G476H, H1*+N54S+V56T+G109A+R116A+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+G109A+R116H+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+P45A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+P124A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+P364A+K391A+C474V+G476K, H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+G50A+T51A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V264F+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y382L+K391A+G476K, H1*+G7K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255A+Q256A+K391A+G476K, H1*+N54S+V56T+G109A+W167Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W167H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+T165V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174Q+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N16H+V17L+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y382F+K391A+G476K, H1*+Q32S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Q385L+K391A+G476K, H1*+N54S+V56T+R87S+G109A+R171H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116H+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+P473R+G476K, H1*+N54S+V56T+G109A+Q169E+Q172G+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+K391A+P473R+G476K, H1*+N54S+V56T+G109A+R116W+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206F+K391A+G476K, H1*+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N260G+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+Y295N+K391A+G476K, H1*+N54S+V56T+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+A186N+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+A288V+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+W48F+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+V291A+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+R320A+S323N+K391A+G476K, H1*+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116H+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N270T+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+N260G+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+A186D+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+A225V+K391A+G476K, H1*+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G255S+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K269Q+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255S+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, G182*+D183*+N195F, H1*+N54S+V56T+G109A+Q169E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+W284H+K391A+G476K, H1*+N54S+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+S304R+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246V+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+N270G+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+Y295F+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174T+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W140Y+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+F328L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G476K, F113Q+R116H+D183*+G182*+N195F, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A288L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N270G+K391A+G476K, Q169E+Q172K+A174*+D183*+G182*+N195F, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K269Q+K391A+G476K, H1*+W48F+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, F113Q+R116Q+D183*+G182*+N195F, N54S+V56T+D183*+G182*+N195F, H1*+N54S+V56T+G109A+A174T+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116W+A174S+G182*+D183*+N195F+V206L+K391A+G476K, Q169E+Q172K+G182*+D183*+N195F, G182*+D183*+A186D, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238A+K391A+G476K, G182*+D183*+A186N, H1*+N54S+V56T+G109A+A174S+V206L+G182*+D183*+K391A+G476K, H1*+N54S+V56T+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206F+K391A+G476K, H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+A186N+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F289L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y295F+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S244Q+K391A+G476K, G182*+D183*+F328L, G182*+D183*+N195F+A288V, G182*+D183*+A186N+N195F, G182*+D183*+E190P+N195F, W140Y+G182*+D183*+N195F, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A225V+K391A+G476K, G182*+D183*+A186D+N195F, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+W48Y+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, G182*+D183*+N195F+V238A, G182*+D183*+Y295N, Q172G+G182*+D183*, F113Q+G182*+D183*+N195F, H1*+N54S+V56T+Q98A+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, G182*+D183*+N195F+Y295F, G182*+D183*+A186E, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206Y+K391A+G476K, G182*+D183*+N195F+W284H, G182*+D183*+A186Q+N195F, K72R+G182*+D183*, D183*+G182*+N195F+V326L, D183*+G182*+A186E+N195F, Q169E+G182*+D183*+N195F, D183*+G182*+N195F+K242Q, G7A+G182*+D183*+N195F, D183*+G182*+N195F+N260G, D183*+G182*+R321A+S323N, D183*+G182*+W284H, H1*+D183*+G182*, D183*+G182*+A186H+N195F, D183*+G182*+F289V, D183*+G182*+K391A, D183*+G182*+S304R, W167F+G182*+D183*+N195F, F113Q+R116H+D183*+G182*, D183*+G182*+N270G, D183*+G182*+S244Q, H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, D183*+G182*+N195F+Y295N, W48F+D183*+G182*, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, D183*+G182*+A186Q, W140Y+D183*+G182*, D183*+G182*+K242Q, D183*+G182*+V206L, D183*+G182*+N195F+V206F, Q169E+D183*+G182*, D183*+G182*+N195F+R320A, G182*+D183*+G184T+N195F, D183*+G182*+G184T, N54S+V56T+D183*+G182*, D183*+G182*+P473R, W48Y+D183*+G182*, D183*+G182*+R323A, G7A+D183*+G182*, G109A+D183*+G182*, W167F+D183*+G182*, H1*+N54S+V56T+G109A+Q169E+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, A174S+D183*+G182*, R116Q+D183*+G182*, D183*+G182*+N195F+V291A, Q172K+D183*+G182*, N54S+D183*+G182*, D183*+G182*+N195F+S323N, D183*+G182*+N195F+M246V, W48Y+D183*+G182*+N195F, D183*+G182*+N195F+S304R, V56T+D183*+G182*, wherein numbering is according to SEQ ID NO: 13; and N54S+G182*+D183*+N195F, G109A+G182*+D183*+N195F, G182*+D183*+N195F+K391A, G182*+D183*+N195F+G476K, H1*+G182*+D183*+N195F, N54S+G182*+D183*+N195F, V56T+G182*+D183*+N195F, G182*+D183*+N195F+I405L, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T+D476K, A174S+G182*+D183*+N195F, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+A174S+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476G, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476K+G477S, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476K, H1*+N54S+V56T+K72R+G109A+W167F+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428− H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S304N+I405L+A421H+A422P+A428T+D476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476K+G477A, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476G+G477T, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+G477Q, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+G477A, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476K+G477Q, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476N, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476Y, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206I+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+A204G+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+A204G+V206L+G255A+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+R116H+T165G+Q172G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+Q172N+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+G196R+V206L+G255A+I405L+A421H+A422P+A428T+N475S, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+T40D+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+N125D+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+A60V+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428− H1*+N54S+V56T+G109A+R116V+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+R116Q+G133Q+T165G+Q172G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+R116Q+Q172G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T+G448D, H1*+N54S+V56T+G109A+F113Q+R116H+Q172N+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+T134E+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+G255A+A265G+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+R116Q+W167F+Q172R+L173V+A174S+G182*+D183*+N195F+V206L+A265G+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+F113Q+T134E+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+G255A+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+G184T+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+G255A+I405L+A421H+A422P+A428− H1*+N54S+V56T+G109A+F113Q+R116Q+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+F113Q+F116H+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+N195F+A204G+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+A265G+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+A37H+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+I405L+A421H H1*+N54S+V56T+G109A+A174S+M105F+G182*+D183*+N195F+V206L+L228I+R320A+S323N+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+F113Q+T134E+A174S+G182*+D183*+N195F+V206L+G255A+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+R116Q+V120L+A174S+G182*+D183*+G184T+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+R116Q+Q172N+A174S+G182*+D183*+N195F+V206L+A265G+I405L+A421H+A422P+A428T H1*+A37H+N54S+V56T+A60V+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, wherein numbering is according to SEQ ID NO: 14.
16. The variant according to claim 1, wherein said variant is selected from the group consisting of; G182*+D183*+N195F+V206F+N260P+L312I+L313I+R320A+T355L+L389I+T400P H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+G50A+T51A+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G476K H1*+N54S+V56T+M105F+G109A+A174S+G182*+D183*+N195F+V206L+R320A+S323N+K391A+G476K H1*+T40K+N54S+V56T+G109A+G149H+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K H1*+N16H+V17L+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+Q22N+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+Q32S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+H28N+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N29S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+H28N+N29S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+A174S+R176K+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+R176K+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+A174S+N175G+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+Y295N+Q299T+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K269S+A274K+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L235I+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L235V+V238A+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Q365S+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+H402R+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320S+H321N+K391A+G476K H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+Q125S+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L235V+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A265G+K391A+G476K H1*+T51Q+N54S+V56T+G109A+A174S+G182*+D183*+N195F+Y203F+V206L+K391A+G476K H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N175Q+G182*+D183*+N195F+V206L+K391A+G476K H1*+H28N+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K H1*+G50A+T51A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+G7K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+M202V+V206L+K391A+G476K H1*+N54S+V56T+M105F+G109A+A174S+G182*+D183*+N195F+V206L+M246F+K391A+G476K H1*+N16H+V17L+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K H1*+T51G+N54S+V56T+G109A+A174S+G182*+D183*+N195F+Y203G+V206L+K391A+G476K H1*+T51G+N54S+V56T+G109A+G110N+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+Q125S+A174S+N175G+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A265G+V291A+F328L+K391A+G476K H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246F+A265G+V291A+F328L+Q365S+K391A+G476K H1*+N16Y+N54S+V56T+G109A+R116H+A174S+G182*+D183*+N195F+V206L+G337E+D377H+K391A+G476K H1*+I9L+N54S+V56T+G109A+Q125S+V131T+A174*+G182*+D183*+V206L+Y267F+F289H+Q361E+K391A+G476K, wherein numbering is according to SEQ ID NO: 13, and wherein said IF is determined by use of Model A detergent.
17. The variant according to claim 1, wherein said variant has an IF of at least >1.5 when compared to said parent polypeptide, and wherein said variant is selected from H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+R87S+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T40G+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72H+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37H+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37M+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37Y+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37R+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113W+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q118N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116W+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116L+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+G142T+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q125P+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q125K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S381G+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246T+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V213T+K391A+G476K, H1*+A37L+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q118K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q125A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377S+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377G+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377R+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377A+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214G+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S381A+K391A+G476K, H1*+N54S+V56T+G109A+W1671+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W167G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346S+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346P+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214S+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214T+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420Q+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420S+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420K+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420L+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391Y+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391R+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473G+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473T+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+Q395P+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444S+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444D+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444Y+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280W+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280T+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280A+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255N+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T285L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T285Q+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+Q449T+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473K+G476K, H1*+N54S+V56T+K72S+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T40K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346T+K391A+G476K, H1*+G50A+N54S+V56T+G109A++Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484A, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484G, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484P, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484E, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484Q, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484S, H1*K+G50A+N54S+V56T+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+L173V+A174S+G182*+D183*+N195F+A204T+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W140Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+Q98S+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+H324L+K391A+G476K, H1*+N54S+V56T+G109A+G149Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346T+K391A+G476K+G477A, H1*+N54S+V56T+G109AG255A++A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+G50A+N54S+V56T+G109A+F113L+R116L+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+G477Q, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Q345D+K391A+G476K+G477Q, H1*+G50A+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V2641+K391A+G423H+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320V+K391A+G476Y, H1*+T5K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L217V+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L217H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+P211D+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391Q+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444H+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444R+G476K, H1*+G50A+N54S+V56T+G109A+L173V+A174S+G182*+D183*+N195F+A204V+V206L+K391A+G476K, H1*+G50A+N54S+V56T+G109A+F113L+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+G7K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K, H1*+N54S+V56T+G109A+Q118G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280K+K391A+G476K, H1*+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+G346T+K391A+G476K+G477A, H1*+N54S+V56T+G109A+Q118S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G337H+Q385L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y382M+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+K72R+M 105F+G109A+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+M105F+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+M105F+G109A+Q118F+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+S323N+D377H+K391A+G476K, H1*+T51E+N54S+V56T+G109A+A174H+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+L173A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Q299V+K391A+G476K, H1*+N54S+V56T+G109A+L173G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T355L+K391A+G476K, H1*+G50A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204T+V206L+K391A+G476K, H1*+G50A+N54S+V56T+G109A+A174E+G182*+D183*+N195F+A204T+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+R320A+S323N+K391A+G476K, H1*+N54S+V56T+M105I+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246L+K391A+G476K, H1*+T51G+N54S+V56T+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174D+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51S+N54S+V56T+G109A+A174P+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72A+G109A+W167S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+W167H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+Q11H+N54S+V56T+G109A+Q118R+T134E+A174S+G184T+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+G109A+Q118R+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+Q172D+A174Q+G182*+D183*+N195F+V206L+K391A+G476K+G477A, H1*+N54S+V56T+K72R+G109A+W167L+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+W167M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K, H1*+N54S+V56T+M 105F+G109A+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+R320A+S323N+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291G+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+F328L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238A+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238T+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238G+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F328M+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F328L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F328V+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F328I+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291T+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+D377H+K391A+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+G109A+L173H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N16S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72N+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72N+G109A+W167L+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72A+G109A+W167Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+G184T+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q118R+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+R320A+S323N+K391A+G476K, H1*+N54S+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L217T+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377S+K391A+G476K, H1*+N54S+V56T+G109A+R116E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L217Q+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377A+K391A+G476K, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346P+K391A+G476K, H1*+N54S+V56T+G109A+R116S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377Q+K391A+G476K, H1*+N54S+V56T+G109A+R1161+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174F+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174T+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+T51A+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51S+N54S+V56T+G109A+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51A+N54S+V56T+G109A+Q172S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51E+N54S+V56T+G109A+Q172T+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51A+N54S+V56T+G109A+Q172A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51S+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51A+N54S+V56T+G109A+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51E+N54S+V56T+G109A+Q172L+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51E+N54S+V56T+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51D+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51A+N54S+V56T+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q172S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72 H+G109A+Q172T+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q172R+A174S+K179E+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q172M+A174S+G182*+D183*+N195F+V206L+Y295N+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q172T+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72M+G109A+Q172S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72S+G109A+A174H+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+A174D+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174D+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+A174K+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174G+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174Q+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+A174G+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72S+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174H+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391S+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444A+G476K, H1*+N54S+V56T+G109A+F113L+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+G50A+N54S+V56T+G109A+A174E+G182*+D183*+N195F+A204T+V206L+S323N+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q118R+A174S+G182*+D 183*+G184T+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+M105F+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q118R+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A225F+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246A+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y3821+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S376H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214E+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214W+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204T+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204S+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+T165S+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T334S+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A263G+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M286F+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y2671+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L235V+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L235A+D377H+K391A+G476K, H1*+N54S+V56T+M105L+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+M105I+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+M105V+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M2461+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L2501+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250V+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391Y+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391M+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391E+G476K, H1*+N54S+V56T+G109A+T165G+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54L+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174V+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246F+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246V+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246S+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174R+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174L+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206S+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391D+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391R+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391H+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391W+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391I+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250T+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250A+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250F+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250M+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391Q+G476K, H1*+N54S+V56T+G109A+Q172H+L173A+A174P+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+W167R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+G184T+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+G255A+D377H+K391A+G476K, H1*+N54S+V56T+G109A+Q118R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S376T+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S376V+K391A+G476K, H1*+N54S+V56T+G109A+Q172E+L1731+A174N+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172D+L173A+A174T+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267M+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267H+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238A+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238T+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238G+K391A+G476K, H1*+N54S+V56T+M105V+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V264T+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V264I+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L235V+K391A+G476K, H1*+N54S+V56T+G109A+W167H+Q172N+L173A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W167F+Q172E+L173P+A174K+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172N+L173T+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172K+L173V+A174T+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172E+L173F+A174R+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172E+L173T+A174R+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172N+L173V+A174R+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+L173V+A174R+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172E+L173M+A174H+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172E+L173F+A174P+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W167Y+Q172E+L173V+A174H+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+L173A+A174T+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+F328L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246S+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250V+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250F+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250M+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204S+V206L+K391A+G476K, H1*+N54S+V56T+G109A+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T334S+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A263G+M286L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267L+K391A+G476K, H1*+T51A+N54S+V56T+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391L+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109S+F113Q+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+A204G+V206L+G255A+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+W167F+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+G184T+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+P473G+G476K, H1*+N54S+V56T+G109A+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+M105F+G109A+A174S+G182*+D183*+N195F+V206L+R320A+S323N+K391A+C474V+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+G255A+K391A+C474V+G476K, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+C474V+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A265G+K391A+T444Q+G476K+G477A, H1*+A37V+N54S+V56T+G109A+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G346T+G477A+G476K, H1*+N54S+V56T+G109A+R116Q+Q172 D+A174S+G182*+D183*+N195F+V206L+G346T+K391A+T444Q+G477A+G476K, H1*+N54S+V56T+G109A+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+G346T+K391A+T444Q+G477A+G476K, H1*+N54S+V56T+G109S+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109S+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109S+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+V264I+K391A+C474V+G476K, H1*+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280Q+H321Y+K391A+C474V+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+C474V+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109A+F113Q+R116Q+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+R116H+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+T165G+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116Q+T165G+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+F2891+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116Q+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116H+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+T165G+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+Q395P+G476K, H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+A265G+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+K72R+G109A+T134E+T165G+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+A265G+K391A+P473R+G476K, H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51V+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280Q+H321Y+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109A+R116Q+W167F+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+G133Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+Q395P+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+K72R+G109A+R116H+W167F+Q172R+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+G184T+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109S+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109S+R116Q+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109S+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+G109S+F113Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+R116H+T134E+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+K72R+G109A+R116H+T134E+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+G255A+K391A+Q395P+T444Q+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+T134E+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+G255A+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+T134E+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+G255A+K391A+Q395P+A445Q+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116H+T134E+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+G255A+K391A+Q395P+G476K, H1*+N54S+V56T+K72R+G109A+T134E+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+G255A+A265G+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+K72R+G109A+G133Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172N+L173V+A174S+G182*+D183*+G184T+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+A37H+N54S+V56T+G109A+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37H+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+T459N+G476K, H1*+N54S+V56T+G109A+F113Q+Q172R+L173V+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+A60V+G109A+R116H+Q172R+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476I H1*+A37H+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476I H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37H+N54S+V56T+A60V+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37H+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116A+W167F+Q172R+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+R116A+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+R116A+T165G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109A+G182*+D183*+N195F+K391A+G476K, H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L, N54S+V56T+G109A+G182*+D183*+N195F, N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+G476K, H1*+N54S+V56T+A174S+G182*+D183*+N195F+V206L+G273R+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L, H1*+W48G+G109A+G182*+D183*+N195F+V206L+K391A+G476K, N54S+V56T+G109A+A174S+G182*+D183*+N195F+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+R116Q+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+G109A+R116A+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+M246F+K391A+G476K, H1*+V56A+G109A+A174S+G182*+D183*+N195F+V206L+G476K, H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+K391A, H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+G476K, G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, N54S+V56T+G109A+G182*+D183*+N195F+V206L+K391T+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116H+A174S+G182*+D183*+G184T+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109S+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+F113Q+R116Q+Q172N+A174S+G182*+D183*+N195F+V206L+A265G+K391A+P473R+G476K, H1*+N54S+V56T+G109A+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+R116A+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109A+R116H+Q172R+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K, H1*+A37V+N54S+V56T+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37H+N54S+V56T+G109A+R116H+T165G+A174S+G182*+D183*+N195F+V206L+M246F+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+K391A+G476K, H1*+G109A+G182*+D183*+N195F+V206L+K391A+G476K, G109A+G182*+D183*+N195F+V206L+G476K, H1*+G109A+G182*+D183*+N195F+K391A, H1*+N54S+V56T+G109A+G133S+A174S+G182*+D183*+N195F+K391A+G476H, H1*+N54S+V56T+G109A+R116A+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+G109A+R116H+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+P45A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+P124A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+P364A+K391A+C474V+G476K, H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+G50A+T51A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V264F+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y382L+K391A+G476K, H1*+G7K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255A+Q256A+K391A+G476K, H1*+N54S+V56T+G109A+W167Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W167H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+T165V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174Q+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N16H+V17L+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y382F+K391A+G476K, H1*+Q32S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Q385L+K391A+G476K, H1*+N54S+V56T+R87S+G109A+R171H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, G109A+G182*+D183*+N195F, G182*+D183*+N195F+K391A, H1*+N54S+V56T+G109A+R116H+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+P473R+G476K, H1*+N54S+V56T+G109A+Q169E+Q172G+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+K391A+P473R+G476K, H1*+N54S+V56T+G109A+R116W+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206F+K391A+G476K, H1*+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N260G+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+Y295N+K391A+G476K, H1*+N54S+V56T+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+A 186N+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+A288V+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+W48F+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+V291A+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+R320A+S323N+K391A+G476K, H1*+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116H+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N270T+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+N260G+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+A186D+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+A225V+K391A+G476K, H1*+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G255S+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K269Q+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255S+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, G182*+D183*+N195F, H1*+N54S+V56T+G109A+Q169E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+W284H+K391A+G476K, H1*+N54S+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+S304R+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246V+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+N270G+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+Y295F+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174T+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W140Y+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+F328L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G476K, F113Q+R116H+D183*+G182*+N195F, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A288L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N270G+K391A+G476K, Q169E+Q172K+A174*+D183*+G182*+N195F, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K269Q+K391A+G476K, H1*+W48F+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, F113Q+R116Q+D183*+G182*+N195F, N54S+V56T+D183*+G182*+N195F, H1*+N54S+V56T+G109A+A174T+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116W+A174S+G182*+D183*+N195F+V206L+K391A+G476K, Q169E+Q172K+G182*+D183*+N195F, G182*+D183*+A186D, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238A+K391A+G476K, G182*+D183*+A186N, H1*+N54S+V56T+G109A+A174S+V206L+G182*+D183*+K391A+G476K, H1*+N54S+V56T+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206F+K391A+G476K, H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+A186N+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F289L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y295F+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+K391A+G476K, G182*+D183*+A186N+N195F, G182*+D183*+A186D+N195F, G182*+D183*+N195F+W284H, D183*+G182*+N195F+V326L, wherein numbering is according to SEQ ID NO: 13, and H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476G+G477T, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+G477Q, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+G477A, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476N, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476Y, H1*+N54S+V56T+G109A+A1745+G182*+D183*+N195F+V2061+1405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+T40D+N54S+V56T+G109A+A1745+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+N125D+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+A60V+G109A+R116Q+A1745+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428− H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+R116H+Q172N+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+T134E+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+G255A+A265G+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+R116Q+W167F+Q172R+L173V+A174S+G182*+D183*+N195F+V206L+A265G+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+F113Q+T134E+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+G255A+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+G184T+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+G255A+I405L+A421H+A422P+A428− H1*+N54S+V56T+G109A+F113Q+R116Q+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+F113Q+F116H+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+R116Q+W167F+Q172G+A1745+G182*+D183*+N195F+A204G+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+A265G+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+A37H+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+I405L+A421H H1*+N54S+V56T+K72R+G109A+F113Q+T134E+A174S+G182*+D183*+N195F+V206L+G255A+I405L+A421H+A422P+A428T, H1*+N54S+V56T+K72R+G109A+R116Q+V120L+A174S+G182*+D183*+G184T+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, H1*+N54S+V56T+G109A+F113Q+R116Q+Q172N+A174S+G182*+D183*+N195F+V206L+A265G+I405L+A421H+A422P+A428T, H1*+A37H+N54S+V56T+A60V+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, wherein numbering is according to SEQ ID NO: 14, and wt determined by use of Model A detergent.
18. The variant according to claim 1, wherein said variant has an IF of at least >2.0 when compared to said parent polypeptide, and wherein said variant is selected from H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+R87S+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T40G+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72 H+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37H+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37M+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37Y+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37R+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113W+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q125P+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S381G+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246T+K391A+G476K, H1*+A37L+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q125A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377S+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377A+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S381A+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346P+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214S+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420Q+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420S+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420K+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420L+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391Y+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473G+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444D+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444Y+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280W+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280L+K391A+G476K, H1*+N54S+V56T+K72S+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T40K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346T+K391A+G476K, H1*+G50A+N54S+V56T+G109A++Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484S, H1*K+G50A+N54S+V56T+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+L173V+A174S+G182*+D183*+N195F+A204T+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W140Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346T+K391A+G476K+G477A, H1*+N54S+V56T+G109AG255A++A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+G50A+N54S+V56T+G109A+F113L+R116L+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320V+K391A+G476Y, H1*+N54S+V56T+G109A+R116M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L217V+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L217H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+P211D+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391Q+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444H+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444R+G476K, H1*+G50A+N54S+V56T+G109A+L173V+A174S+G182*+D183*+N195F+A204V+V206L+K391A+G476K, H1*+G7K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K, H1*+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+G346T+K391A+G476K+G477A, H1*+N54S+V56T+G109A+Q118S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y382M+K391A+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+K72R+M 105F+G109A+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+M105F+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+S323N+D377H+K391A+G476K, H1*+T51E+N54S+V56T+G109A+A174H+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+L173A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Q299V+K391A+G476K, H1*+N54S+V56T+G109A+L173G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T355L+K391A+G476K, H1*+G50A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204T+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+R320A+S323N+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246L+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+W167H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+G109A+Q118R+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+Q172D+A174Q+G182*+D183*+N195F+V206L+K391A+G476K+G477A, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238T+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+D377H+K391A+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+G109A+L173H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72N+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72N+G109A+W167L+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+G184T+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q118R+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K, H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377S+K391A+G476K, H1*+N54S+V56T+G109A+R116E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+R116S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R1161+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51A+N54S+V56T+G109A+Q172S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51E+N54S+V56T+G109A+Q172T+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51S+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51E+N54S+V56T+G109A+Q172L+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51E+N54S+V56T+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q172S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72 H+G109A+Q172T+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+Q172T+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72M+G109A+Q172S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K H1*+N54S+V56T+K72S+G109A+A174H+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+A174D+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174D+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+A174K+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174G+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174Q+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72Q+G109A+A174G+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72S+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174H+G182*+D183*+N195F+V206L+K391A+G476K, H1*+G50A+N54S+V56T+G109A+A174E+G182*+D183*+N195F+A204T+V206L+S323N+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y382I+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214W+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204S+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T334S+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A263G+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M286F+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L235V+D377H+K391A+G476K, H1*+N54S+V56T+M105L+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+M 105I+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+M105V+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M2461+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L2501+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250V+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391Y+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391M+G476K, H1*+N54S+V56T+G109A+T165G+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K, H1*+N54S+V56T+G109S+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174V+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246F+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246V+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246S+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391R+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391H+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391W+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K3911+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250T+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250A+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250F+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250M+D377H+K391A+G476K, H1*+N54S+V56T+G109A+Q172H+L173A+A174P+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+G255A+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S376T+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S376V+K391A+G476K, H1*+N54S+V56T+G109A+Q172E+L1731+A174N+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172D+L173A+A174T+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267M+D377H+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238G+K391A+G476K, H1*+N54S+V56T+M105V+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V264T+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V2641+K391A+G476K, H1*+N54S+V56T+G109A+W167H+Q172N+L173A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172N+L173T+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172E+L173M+A174H+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W167Y+Q172E+L173V+A174H+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+L173A+A174T+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+F328L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250V+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250F+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250M+K391A+G476K, H1*+N54S+V56T+G109A+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51A+N54S+V56T+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391L+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109S+F113Q+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+A204G+V206L+G255A+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+W167F+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+G184T+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+P473G+G476K, H1*+N54S+V56T+G109A+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+M105F+G109A+A174S+G182*+D183*+N195F+V206L+R320A+S323N+K391A+C474V+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+G255A+K391A+C474V+G476K, H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+C474V+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A265G+K391A+T444Q+G476K+G477A, H1*+A37V+N54S+V56T+G109A+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G346T+G477A+G476K, H1*+N54S+V56T+G109A+R116Q+Q172 D+A174S+G182*+D183*+N195F+V206L+G346T+K391A+T444Q+G477A+G476K, H1*+N54S+V56T+G109A+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+G346T+K391A+T444Q+G477A+G476K, H1*+N54S+V56T+G109S+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109S+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+V2641+K391A+C474V+G476K, H1*+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280Q+H321Y+K391A+C474V+G476K, H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+C474V+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109A+F113Q+R116Q+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+R116H+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+T165G+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116Q+T165G+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+F2891+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116Q+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116H+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+A265G+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+K72R+G109A+T134E+T165G+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+A265G+K391A+P473R+G476K, H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+T51V+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280Q+H321Y+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109A+R116Q+W167F+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+G133Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+Q395P+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+K72R+G109A+R116H+W167F+Q172R+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+G184T+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109S+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109S+R116Q+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109S+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+G109S+F113Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+R116H+T134E+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+K72R+G109A+R116H+T134E+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+G255A+K391A+Q395P+T444Q+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+T134E+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+G255A+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116H+T134E+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+G255A+K391A+Q395P+G476K, H1*+N54S+V56T+K72R+G109A+T134E+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+G255A+A265G+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+K72R+G109A+G133Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172N+L173V+A174S+G182*+D183*+G184T+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+A37H+N54S+V56T+G109A+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+T459N+G476K, H1*+N54S+V56T+G109A+F113Q+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+A60V+G109A+R116H+Q172R+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476I H1*+A37H+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476I H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37H+N54S+V56T+A60V+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37H+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116A+W167F+Q172R+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+R116A+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+R116A+T165G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109A+G182*+D183*+N195F+K391A+G476K, H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L, N54S+V56T+G109A+G182*+D183*+N195F, N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+G476K, H1*+N54S+V56T+A174S+G182*+D183*+N195F+V206L+G273R+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L, N54S+V56T+G109A+A174S+G182*+D183*+N195F+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+R116Q+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+G109A+R116A+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+M246F+K391A+G476K, H1*+V56A+G109A+A174S+G182*+D183*+N195F+V206L+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116H+A174S+G182*+D183*+G184T+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109S+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+F113Q+R116Q+Q172N+A174S+G182*+D183*+N195F+V206L+A265G+K391A+P473R+G476K, H1*+N54S+V56T+G109A+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+R116A+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K, H1*+N54S+V56T+G109A+R116H+Q172R+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K, H1*+A37V+N54S+V56T+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37H+N54S+V56T+G109A+R116H+T165G+A174S+G182*+D183*+N195F+V206L+M246F+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+K391A+G476K, H1*+N54S+V56T+G109A+G133S+A174S+G182*+D183*+N195F+K391A+G476H, H1*+N54S+V56T+G109A+R116A+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+A37V+N54S+V56T+G109A+R116H+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+P45A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+P364A+K391A+C474V+G476K, H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+G50A+T51A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V264F+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y382L+K391A+G476K, H1*+G7K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255A+Q256A+K391A+G476K, H1*+N54S+V56T+G109A+W167Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+W167H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+T165V+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174Q+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N16H+V17L+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y382F+K391A+G476K, H1*+Q32S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Q385L+K391A+G476K, H1*+N54S+V56T+R87S+G109A+R171H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+R116H+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+P473R+G476K, H1*+N54S+V56T+G109A+Q169E+Q172G+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+K391A+P473R+G476K, H1*+N54S+V56T+G109A+R116W+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206F+K391A+G476K, H1*+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N260G+K391A+G476K, H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+G184T+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+Y295N+K391A+G476K, H1*+N54S+V56T+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+A186N+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+A288V+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G255A+K391A+G476K, H1*+N54S+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+W48F+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+V291A+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+R320A+S323N+K391A+G476K, H1*+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+K72R+G109A+F113Q+R116H+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N270T+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+N260G+K391A+G476K, H1*+N54S+V56T+G109A+F113Q+R116H+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+A174S+A186D+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+A225V+K391A+G476K, H1*+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G255S+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K269Q+K391A+G476K, H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255S+K391A+G476K, H1*+N54S+V56T+G109A+Q169E+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K; whereing numbering is according to SEQ ID NO: 13, and H1*+T40D+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T H1*+N54S+V56T+G109A+F113Q+N125D+A1745+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T H1*+N54S+V56T+G109A+F113Q+R116H+Q172N+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T H1*+N54S+V56T+K72R+G109A+T134E+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+G255A+A265G+I405L+A421H+A422P+A428T H1*+N54S+V56T+K72R+G109A+R116Q+W167F+Q172R+L173V+A174S+G182*+D183*+N195F+V206L+A265G+I405L+A421H+A422P+A428T H1*+N54S+V56T+K72R+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T H1*+N54S+V56T+K72R+G109A+F113Q+T134E+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+G255A+I405L+A421H+A422P+A428T H1*+N54S+V56T+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+N195F+A204G+V206L+I405L+A421H+A422P+A428T H1*+N54S+V56T+K72R+G109A+R116Q+V120L+A174S+G182*+D183*+G184T+N195F+V206L+I405L+A421H+A422P+A428T H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T H1*+N54S+V56T+G109A+F113Q+R116Q+Q172N+A174S+G182*+D183*+N195F+V206L+A265G+I405L+A421H+A422P+A428T H1*+A37H+N54S+V56T+A60V+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, wherein numbering is according to SEQ ID NO: 14, and where said IF is determined by use of Model A detergent.
19. The variant according to claim 1 wherein said variant is selected from a group consisting of: H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476KH1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476KH1*+N54S+V56T+G109A+R116H+A174S+G182*+D183*+N195F+V206L+K391A+G476KH1*+N54S+V56T+G109A+R116Q+W167F+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476KH1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476KH1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476KH1*+N54S+V56T+G109A+F113Q+R116Q+Q172N+A174S+G182*+D183*+N195F+V206L+A265G+K391A+P473R+G476KH1*+N54S+V56T+K72R+G109A+G133Q+W167F+Q172R+A1745+G182*+D183*+N195F+V206L+K391A+G476KH1*+N54S+V56T+K72R+G109A+R116H+T134E+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, andH1*+N54S+V56T+K72R+G109A+R116H+T134E+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+G255A+K391A+Q395P+T444Q+P473R+G476Kwherein numbering is according to SEQ ID NO: 13.
20. A composition comprising a variant according to claim 1.
21. The composition according to claim 20, wherein said composition further comprises at least one further active component.
22. The composition according to claim 20, wherein said further active component is an enzyme, such as a protease, lipase, cellulose, pectate lyase and mannanase.
23. The composition according to claim 20, which is a liquid laundry or liquid dish wash composition, such as an Automatic Dish Wash (ADW) liquid detergent composition, or a powder laundry, such as a soap bar, or powder dish wash composition, such as an ADW unit dose detergent composition and such as a Hand Dish Wash (HDVV) detergent composition.
24. The composition according to claim 20, wherein said composition further comprises a surfactant, builder, bleach, polymer, or wherein said composition is phosphate-free.
25. An polynucleotide encoding the variant according to claim 1.
26. A nucleic acid construct comprising the polynucleotide according to claim 25.
27. An expression vector comprising the polynucleotide according to claim 25.
28. A host cell comprising the polynucleotide according to claim 25.
29. A method of producing an alpha-amylase variant, comprising: a. cultivating the host cell of claim 28 under conditions suitable for expression of said variant; andb. recovering said variant.
30. A method of improving wash performance of a parent polypeptide having the amino acid sequence of SEQ ID NO: 13 or 14, or having at least 80% sequence identity thereto, said method comprising the steps of: a) a substitution and/or deletion of two, three or four positions in the parent alpha-amylase said positions corresponding to positions 181, G182, D183, and G184 of the mature polypetide of SEQ ID NO: 4; andb) an alteration at one or more positions corresponding to positions positions H1, T5, G7, Q11, N16, V17, Q32, A37, T40, P45, W48, G50, T51, N54, V56, K72, R87, Q98, M105, G109, F113, R116, Q118, Q125, G133, T134, W140, G142, G149, T165, W167, Q169, R171, Q172, L173, A174, G184, A186, E190, T193, N195, A204, V206, P211, I214, V215, L217, L219, A225, L235, V238, K242, S244, M246, M248, L250, G255, Q256, N260, A263, V264, Y267, K269, N270, G273, S280, W284, T285, M286, F289, V291, N295, Q299, R320, H321, S323, H324, V326, F328, T334, D337, Q345, G346, G348, T355, S376, D377, D379, Y382, S383, Q385, K391, K393, Q395, A420, G423, T444, A445, Q449, T459, P473, C474, G476, G477, and K484 of SEQ ID NO: 13; or H1, A37, T40, N54, V56, A60, K72, G109, F113, R116, N125, F133, T134, T165, Q169, Q172, L173, A174, G184, A186, E190, N195, G196, A204, V206, A225, L228, K242, S244, G255, N260, A265, K269, S280, W284, N295, S304, R320, H321, S323, V326, K391, I405, A421, A422, A428, G448, D476, and G477 of SEQ ID NO: 14,
31. The method according to claim 30, wherein said variant has at least 50%, such as at least 60%, or at least 70%, or at least 80%, or at least 90%, or at least 100% of the activity of said parent polypeptide having the amino acid sequence of SEQ ID NO: 7.
32. The method according to claim 30, wherein said activity is determined according to a Phadebas assay.
33. (canceled)

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Number	Date	Country	Kind
2335/CHE/2015	May 2015	IN	national

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Filing Document	Filing Date	Country	Kind
PCT/EP2016/060266	5/9/2016	WO	00

ALPHA-AMYLASE VARIANTS AND POLYNUCLEOTIDES ENCODING SAME

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