Claims
- 1. A method for producing a polypeptide comprising:transforming or transfecting a cell with a vector containing: a) a polynucleotide encoding a polypeptide of SEQ ID NO:2; or b) a polynucleotide which encodes a polypeptide that is at least 70% identical to a polypeptide of SEQ ID NO:2 having amidase activity, such that the cell expresses the polypeptide encoded by the polynucleotide.
- 2. The method of claim 1, wherein the vector is an expression vector.
- 3. The method of claim 1, wherein the polynucleotide is set forth in SEQ ID NO:1.
- 4. The method of claim 2, wherein the vector is a plasmid.
- 5. The method of claim 2, wherein the vector is virus-derived.
- 6. The method of claim 1, wherein the cell is a prokaryote.
- 7. The method of claim 1, wherein the cell is a eukaryote.
CROSS-RELATED APPLICATIONS
This application is a divisional of U.S. application Ser. No. 09/427,372, filed Oct. 25, 1999, which is a divisional of U.S. application Ser. No. 09/261,006, filed Mar. 2, 1999, now issued U.S. Pat. No. 6,004,796, which is Div. of Ser. No. 09/066,285, now U.S. Pat. No. 5,985,646, which is CON of Ser. No. 08/951,088, filed Oct. 15, 1997, now U.S. Pat. No. 6,136,583 which is a divisional of U.S. application Ser. No. 08/664,646, filed Jun. 17, 1996, now issued U.S. Pat. No. 5,877,001, the contents of which are hereby incorporated by reference.
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Continuations (1)
|
Number |
Date |
Country |
Parent |
08/951088 |
Oct 1997 |
US |
Child |
09/066285 |
|
US |