Claims
- 1. A method for removal of arginine, phenylalanine or methionine from the N-terminal end of peptides in peptide or peptidomimetic synthesis, comprising:contacting a polypeptide comprising at least 30 consecutive amino acid residues homologous with an enzyme comprising an amino acid sequence which is at least 70% identical to the amino acid sequence set forth in SEQ ID NO:2, which is effective for removal of arginine, phenylalanine or methionine from the N-terminal end of peptides in peptide or peptidomimetic synthesis, wherein the enzyme has amidase activity.
- 2. The method of claim 1, wherein the enzyme comprises an amino acid sequence which is at least 90% identical to the amino acid sequence set forth in SEQ ID NO:2.
- 3. The method of claim 1, wherein the enzyme comprises an amino acid sequence which is at least 95% identical to the amino acid sequence set forth in SEQ ID NO:2.
CROSS-RELATED APPLICATIONS
This application is a divisional and claims the benefit of priority under 35 USC §120 of U.S. application Ser. No. 09/427,372, filed Oct. 25, 1999 (pending), which is a divisional of U.S. Pat No. 09/261,006 filed Mar. 2, 1999, now 6,004,796, which is a divisional of U.S. patent application Ser. No. 09/066,285, filed Apr. 24, 1998 (issued on Nov. 16, 1999 as U.S. Pat. No. 5,985,646); which is a continuation of U.S. application Ser. No. 08/951,088, filed Oct. 15, 1997; Now U.S. Pat. No 6,136,583; which is a divisional of U.S. application Ser. No. 08/664,646 filed Jun. 17, 1996 (issued on Mar. 2, 1999 as U.S. Pat. No.5,877,001). The disclosure of the prior applications is considered part of and is incorporated by reference in the disclosure of this application.
US Referenced Citations (2)
Number |
Name |
Date |
Kind |
5451522 |
Queener et al. |
Sep 1995 |
A |
5494796 |
Spears et al. |
Feb 1996 |
A |
Non-Patent Literature Citations (1)
Entry |
W70508—Sequence search alighnment between Applicants' SEQ ID No. : 2 and AC- W70508. |
Continuations (1)
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Number |
Date |
Country |
Parent |
08/951088 |
Oct 1997 |
US |
Child |
09/066285 |
|
US |