Amino Lipid and Preparation Method and Application Thereof

Abstract

The disclosure belongs to the technical field of medical chemistry, particularly relates to an amino lipid and their preparation method as well as an application thereof, and provides the ionizable amino lipids with a general formula as shown in Formula (I), or pharmaceutically acceptable salts thereof. The amino lipids of the disclosure can be used for delivering nucleic acids and small molecule drugs. The amino lipid compounds have two ester bonds, which obviously enhance the lysosome escape capability of the ionizable amino lipids, and are favorable for the release of delivery targets of a targeted drug or gene, etc., thus improving the delivery efficiency, and showing the good capability of delivering nucleic acids into cells in the in-vitro and in-vivo delivery study.

Description

TECHNICAL FIELD

The disclosure relates to the technical field of medical chemistry, in particular to an amino lipid and its preparation method as well as an application thereof.

BACKGROUND

Nucleic acid drugs have very wide application prospects in aspects of prevention and treatment of cancer, infectious diseases, genetic diseases, and cardiovascular diseases. However, RNA, DNA, and siRNA etc. are easily degraded in vivo, and the bioavailability is very low when direct administration of oral administration or intravenous injection is used. Therefore, the delivery by vectors is required.

Commonly used nucleic acid vectors include viral vectors and non-viral vectors. Viral vectors have high transfection efficiency, but they lack targeted performance, and have greater safety concerns, low vector capacity and high production cost. Non-viral vectors have the advantages of high safety, easy modification of vector molecules, etc., are suitable for mass production, and have wide application prospects. The application of an LNP (Lipid Nanoparticles) delivery system plays a leading role. The LNP generally consists of ionizable or cationic lipids, phosphonates, cholesterol and pegylated lipids. All of them are amphiphilic molecules with self-assembly performance in structure, and LNP has been widely concerned because of its determined structure of each ingredient, good reproducibility, easy quality supervision, long in-vivo circulation time, good biocompatibility, etc. After entering the cells, the nanoparticles need to escape from the endosome/lysosome to release RNA in the cytoplasm, so that it can be expressed to produce the target protein. However, the escape rate of the LNP from the endosome/lysosome is generally low at present. Although DLin-MC3-DMA, as the “gold standard” for evaluation in amino lipids, is the most efficient amino lipid at present, and is approved by FDA for the first siRNA therapeutic drug Onpattro (patisiran), but only 1%-4% of RNA escapes from the endosome/lysosome. The escape from the endosome/lysosome has become a key step affecting nucleic acid delivery. Therefore, it is of great research significance and practical need to design an amino lipid with good nucleic acid entrapping capacity and high escape capacity from the endosome/lysosome to solve the nucleic acid delivery problem.

SUMMARY

By aiming at the technical problems of low transfection efficiency, cytotoxicity due to positive charges, etc. in the prior art, the disclosure provides an amino lipid and an application thereof.

The objective of the disclosure is achieved through the following technical solution:

In a first aspect, the disclosure provides:

An amino lipid with a structure as shown in Formula (I):

In Formula (I), L is C₁-C₆ alkylene, C₁-C₆ alkenylene, C₁-C₆ alkynylene, C₃-C₆ cycloalkylene and C₃-C₆ cycloalkenylene; R¹ and R² are identical or different, and are each independently selected from C₁-C₂₀ alkyl, C₁-C₂₀ alkenyl, C₁-C₂₀ alkynyl, C₁-C₂₀ cycloalkyl, C₁-C₂₀ cycloalkenyl and C₁-C₂₀ cycloalkynyl; the C₁-C₂₀ alkyl, C₁-C₂₀ alkenyl, C₁-C₂₀ alkynyl, C₁-C₂₀ cycloalkyl, C₁-C₂₀ cycloalkenyl and C₁-C₂₀ cycloalkynyl are able to be optionally substituted by H, C₁-C₆ hydrocarbyl and F;

R³ and R⁴ are identical or different, and are each independently selected from H, C₁-C₁₀ alkyl, C₂-C₁₀ alkenyl and C₂-C₁₀ alkynyl; the C₁-C₁₀ alkyl, C₂-C₁₀ alkenyl and C₂-C₁₀ alkynyl are able to be optionally substituted by C₁-C₆ hydrocarbyl; or

R³ and R⁴ are connected to form a 4 to 10-membered heterocyclic ring, the multi-membered heterocyclic ring includes 1 to 6 heteroatoms, and the heteroatoms are selected from N, S and O.

Preferably, R¹ is selected from C₄-C₁₇ alkyl, C₄-C₁₇ alkenyl, C₄-C₁₇ alkynyl, C₄-C₁₇ cycloalkyl, C₄-C₁₇ cycloalkenyl and C₄-C₁₇ cycloalkynyl; the C₄-C₁₇ alkyl, C₄-C₁₇ alkenyl, C₄-C₁₇ alkynyl, C₄-C₁₇ cycloalkyl, C₄-C₁₇ cycloalkenyl and C₄-C₁₇ cycloalkynyl are able to be optionally substituted by H, C₁-C₆ hydrocarbyl and F.

Preferably, the R¹ is one selected from E1, E2, E3, E4, E5, E6, E7, E8, E9, E10, E11, E12, E13, E14, E15, E16, E17, E18, E19, E20, E21, E22, E23, E24 and E25:

More preferably, the R¹ is one selected from E3, E4, E5, E6, E7, E8, E9, E10, E11, E12, E13, E15, E17, E18, E20, E21 and E24.

Preferably, the R² is selected from C₅-C₁₉ alkyl, C₅-C₁₉ alkenyl, C₅-C₁₉ alkynyl, C₅-C₁₉ cycloalkyl, C₅-C₁₉ cycloalkenyl and C₅-C₁₉ cycloalkynyl; the C₅-C₁₉ alkyl, C₅-C₁₉ alkenyl, C₅-C₁₉ alkynyl, C₅-C₁₉ cycloalkyl, C₅-C₁₉ cycloalkenyl and C₅-C₁₉ cycloalkynyl are able to be optionally substituted by H, C₁-C₆ hydrocarbyl and F.

Preferably, the R² is one selected from C1, C2, C3, C4, C5, C6, C7, C8, C9, C10, C11, C12, C13, C14, C15, C16, C17, C18, C19, C20, C21, C22, C23, C24, C25, C26, C27, C28, C29, C30, C31, C32, C33, C34, C35, C36, C37, C38, C39, C40, C41, C42, C43, C44, C45, C46, C47, C48, C49, C50, C51, C52, C53, C54, C55, C56, C57, C58, C59, C60, C61, C62, C63, C64, C65, C66, C67, C68, C69, C70, C71, C72, C73, C74, C75, C76, C77, C78, C79, C80, C81, C82, C83, C84, C85, C86, C87, C88, C89, C90, C91, C92, C93, C94, C95, C96, C97, C98, C99, C100, C101, C102, C103, C104, C105, C106, C107, C108, C109, C110, C111, C112, C113, C114, C115, C116, C117, C118, C119, C120, C121, C122, C123, C124, C125, C126, C127, C128, C129, C130, C131, C132, C133, C134, C135, C136, C137, C138, C139, C140, C141, C142, C143, C144, C145, C146, C147:

More preferably, the R² is one selected from C3, C4, C5, C6, C7, C8, C9, C10, C11, C12, C13, C14, C56, C57, C58, C60, C62, C63, C64, C66, C67, C71, C72, C74, C79, C82, C83, C102, C103, C104, C105, C106, C107, C108, C109, C110, C111, C112, C113, C114, C115, C116, C117, C118, C119, C120, C121, C122, C123, C124, C125, C126, C127, C128, C129, C130, C131, C132, C133, C134, C135, C136, C137, C138, C139, C140, C141, C142, C143, C144, C145, C146, C147.

Preferably, R³, R⁴ and L form an R³R⁴—N-L amine-containing carboxylic acid structure of

and/or R³ and R⁴ are connected to form a 4 to 10-membered heterocyclic ring, the multi-membered heterocyclic ring includes 1 to 6 heteroatoms, and the heteroatoms are selected from N, S or O.

Preferably,

is one selected from A1, A2, A3, A4, A5, A6, A7, A8, A9, A10, A11, A12, A13, A14, A15, A16, A17, A18, A19, A20, A21, A22, A23, A24, A25, A26, A27, A28, A29, A30, A31, A32, A33, A34, A35, A36, A37, A38, A39, A40:

More preferably,

is one selected from A1, A7, A8, A9, A10, A11, A12, A13, A14, A15, A16, A23, A24, A28, A33 and A37:

Preferably, the amino lipid is one selected from compounds as shown by the following structures:

In a second aspect, the disclosure provides:

a preparation method of the amino lipid according to the first aspect of the disclosure, including the following steps:

• • S1: taking a solvent-free reaction on a compound of R²COOH and an epoxide compound under the catalysis of FeCl₃/Py;
  • S2: adding R³R⁴NLCOOH into the reaction system of S1, and taking a reaction under the condition of existence of a condensation agent to obtain the amino lipid.

The reaction process is described as follows:

Preferably, the method includes the following steps:

• • (1) the first intermediate was obtained through the reaction of an epoxide compound with a compound expressed by R²COOH at room temperature under the catalysis of FeCl₃ and Py; and
  • (2) the first intermediate was separated, a catalytic amount of DMAP was added under the effect of a condensation agent so that the first intermediate and COOH of R³R⁴NLCOOH took a second reaction at room temperature to obtain the amino lipid compound as shown in Formula I. The condensation agent used in the preparation method was EDC·HCl, DCC, etc.

In a third aspect, the disclosure provides:

• • an application of the amino lipid according to the first aspect of the disclosure, and a pharmaceutically acceptable salt, prodrug or stereoisomer of the amino lipid in the preparation of drugs for gene therapy, genetic vaccination, antisense therapy or RNA interference therapy.

Preferably, the drug is used for treating cancer or genetic diseases.

Preferably, the tumor includes but is not limited to gastric cancer, liver cancer, esophagus cancer, colorectal cancer, pancreatic cancer, cerebral cancer, lymph cancer, leukemia, bladder cancer or prostatic cancer. The genetic diseases include but are not limited to hemophilia, thalassemia or Gaucher diseases.

Preferably, the drug is used for treating cancer, allergy, toxicity and pathogen infection.

Preferably, the application is the application for preparation of nucleic acid transfer drugs.

Preferably, the nucleic acid is RNA, including but not limited to mRNA, antisense oligonucleotide, DNA, plasmid, rRNA, miRNA, tRNA, siRNA and snRNA.

In a fourth aspect, the disclosure provides:

• • a nanoparticle delivery system having a raw material of the amino lipid according to the first aspect of the disclosure.

Compared with the prior art, the disclosure has the following technical effects:

The ionizable amino lipid as shown in Formula (I)

disclosed by the disclosure or the pharmaceutically acceptable salt thereof achieves mild reaction conditions in an amino lipid construction process, does not need protection or deprotection, and realizes high atom economy. In in-vitro and in-vivo delivery study, the excellent capability of delivering the nucleic acid to cells is shown. The amino lipid compound has two ester bonds. Due to the introduction of the ester group, the degradation ability of cationic polymers is obviously enhanced, the cell toxicity is greatly reduced, meanwhile, the release of delivery targets such as target drugs or genes can be facilitated, and the delivery efficiency is further improved. The preparation method of the amino lipid compound has the advantages of easy acquisition of raw materials, mild reaction conditions, good reaction selectivity, high reaction yield, low instrument equipment requirement and simple operation.

BRIEF DESCRIPTION OF FIGURES

FIG. 1 is a ¹H-NMR spectrum of E7C71A9 in Embodiment 5.

FIG. 2 is a ¹³C-NMR spectrum of E7C71A9 in Embodiment 5.

FIG. 3 is a tumor growth curve diagram of tumor-bearing mice after receiving intramuscular injection of OVA mRNA vaccines in Embodiment 15 (LNPs assembled by E6C71A12 and E7C71A9 are respectively used).

FIG. 4 is a survival curve of tumor-bearing mice after receiving intramuscular injection of OVA mRNA vaccines in Embodiment 15 (LNPs assembled by E6C71A12 and E7C71A9 are respectively used).

FIG. 5 is a tumor growth curve diagram of tumor-bearing mice after receiving intramuscular injection of OVA mRNA vaccines in Embodiment 23 (LNP assembled by E7C115A11 is used).

FIG. 6 is a survival curve of tumor-bearing mice after receiving intramuscular injection of OVA mRNA vaccines in Embodiment 23 (LNP assembled by E7C115A11 is used).

DETAILED DESCRIPTION

Specific implementations of the discloses are further described below. It needs to be noted that the description of these implementations provided is intended to help to understand the disclosure, but not intended to limit the scope of the disclosure. Furthermore, the technical features involved in the various implementations of the disclosure described below can be combined with each other as long as they do not conflict with each other.

Test methods used in following experimental examples are all conventional methods unless otherwise specified. Used materials, reagents, etc. are commercially available materials and reagents unless otherwise specified.

The term “optionally substituted”, as used herein, means that one or more hydrogen atoms attached to an atom or group is independently unsubstituted or substituted by one or more, for example, one, two, three or four, substituents. When an atom or group is substituted by a plurality of substituents, the plurality of substituents may be identical or different.

Abbreviations herein:

RNA         Ribonucleic acid
DSPC        Distearoyl phosphatidyl choline
DOPE        Dioleoyl phosphatidyl ethanolamine
DOPC        Dioleoyl phosphatidyl choline
DOPS        Dioleoy1 phosphatidyl serine
DSPE        Distearoyl phosphatidyl ethanolamine
PEG2000-DMG (1-(monomethoxypolyethylene glycol)-2,3
            dimyristoyl-glycerol
kD          Kilodalton
PBS         Phosphate buffer solution

In the following embodiments, a general structure formula of the amino lipid is shown in Formula (I)

unless otherwise specified. For the amino lipid structures represented by serial numbers, E1-E25 are the above defined R¹ substituents, C1-C14 7 are the above defined R² substituents, and A1-A40 are the above defined

groups. For example, the structure formula of

Embodiment 1: Parallel Synthesis and Characterization of E7C71Ay Series Amino Lipid Compound Library

FeCl₃ (4 mg, 0.005 mmol), Py (1 μL, 0.0025 mmol), 2-hexyldecanoic acid (0.3 mL, 1 mmol) and 1,2-cyclododecane epoxide (0.27 mL, 1.2 mmol) were sequentially added into a 25 mL reaction tube, and then the reaction was stirred at room temperature overnight to obtain Step I (1 mmol). 10 mL of DCM was added to prepare 0.1 M of a Step I solution.

The Step I solution was respectively transferred into a 1.5 mL 96-well plate (0.1 mL for each, 0.01 mmol) by a pipette, a DCM solution (0.1 mL, 0.02 mmol, 0.2 M) of tertiary amine group-containing carboxylic acid, DIPEA, a DCM solution (0.2 mL, 0.04 mmol, 0.2 M) of EDC·HCl and a DCM solution (0.1 mL, 0.005 mmol, 0.05 M) of DMAP were respectively added into each well, then the mixture was stirred for 6 h at room temperature, and no Step I raw material was observed by TLC detection. After the reactions were completed, the solution was volatilized at room temperature to dryness, and 15 amino lipid compounds E7C71Ay were obtained. Mass spectrometric detection was performed, and the results were collected as shown in Table 1.

TABLE 1
MW/z value of E7C71Ay series amino lipid compound library
	Serial					Measured
	number of		Molecular	Molecular	Calculated	value
	compound	Structure	formula	weight	value M	(M + H)+
1	E7C71A1		C32H63NO4	525.9	525.5	526.6
2	E7C71A7		C35H69NO4	567.9	567.5	568.6
3	E7C71A8		C34H67NO4	553.9	553.5	554.7
4	E7C71A9		C34H67NO4	553.9	553.5	554.7
5	E7C71A10		C35H69NO4	567.9	567.5	568.6
6	E7C71A11		C35H69NO4	567.9	567.5	568.7
7	E7C71A12		C36H71NO4	582.0	581.5	582.6
8	E7C71A14		C34H65NO4	551.9	551.5	552.7
9	E7C71A15		C34H65NO4	551.9	551.5	552.7
10	E7C71A16		C34H65NO4	551.9	551.5	552.6
11	E7C71A23		C35H67NO4	565.9	565.5	566.7
12	E7C71A24		C36H69NO4	580.0	579.5	580.6
13	E7C71A28		C36H69NO4	580.0	579.5	580.6
14	E7C71A33		C35H67NO4	565.9	565.5	566.7
15	E7C71A37		C35H67NO5	581.9	581.5	582.7

Embodiment 2: 2-hydroxyhexadecyl dodecanoate

FeCl₃ (20 mg, 0.025 mmol), Py (5 μL, 0.0125 mmol), dodecanoic acid (1 g, 5 mmol) and 2-epoxy hexadecane (1.7 mL, 6 mmol) were sequentially added into a 25 mL reaction tube, and then the reaction was stirred at room temperature overnight. Column chromatography gradient elution purification (hexane:EA=20:1 to 5:1) was performed to obtain 2-hydroxyhexadecyl dodecanoate (2.0 g, 90% yield). ¹H NMR (400 MHz, CDCl₃): δ 0.88 (t, 6H), 1.26-1.45 (m, 40H), 1.47 (m, 2H), 1.63 (m, 2H), 2.02 (m, 1H), 2.34 (t, 2H), 3.82 (m, 1H), 3.95 (m, 1H), 4.13 (m, 1H). ESI-MS calculated for C₂₈H₅₇O₃⁺ [M+H]⁺ 441.4, found 441.6.

Embodiment 3: 2-((4-(dimethylamino)butanoyl)oxy)hexadecyl dodecanoate

EDC·HCl (192 mg, 1 mmol), DIPEA (174 μL, 1 mmol), DMAP (3.0 mg, 0.025 mmol), 4-(dimethylamino)butanoic acid (101 mg, 0.6 mmol), 2-hydroxyhexadecyl dodecanoate (220 mg, 0.5 mmol) and DCM (4 mL) were sequentially added into a 10 mL reaction tube. The reaction was stirred at room temperature for 3 h to obtain a compound E11C7A9 (235 mg, 85% yield). ¹H NMR (400 MHz, CDCl₃): δ 0.88 (t, 6H), 1.25-1.45 (m, 40H), 1.58 (m, 4H), 1.78 (m, 2H), 2.23 (s, 6H), 2.30 (m, 6H), 4.01 (m, 1H), 4.21 (m, 1H), 5.08 (m, 1H). ¹³C NMR (100 MHz, CDCl₃): δ 14.03, 14.08, 22.59, 22.64, 23.35, 25.11, 27.39, 27.43, 29.23, 29.27, 29.29, 29.45, 29.53, 29.59, 30.80, 31.65, 31.85, 31.89, 32.16, 32.39, 47.39, 47.69, 58.86, 64.49, 71.53, 171.87, 173.43. ESI-MS calculated for C₃₄H₆₈NO₄⁺ [M+H]⁺ 554.5, found 554.7.

Embodiment 4: 2-hydroxydodecyl-2-hexyldecanoate

FeCl₃ (20 mg, 0.025 mmol), Py (5 μL, 0.0125 mmol), 2-hexyldecanoic acid (1.3 g, 5 mmol) and 1,2-epoxydodecane (1.3 mL, 6 mmol) were sequentially added into a 25 mL reaction tube. Then the reaction was stirred at room temperature overnight, and column chromatography gradient elution purification (hexane:EA=20:1 to 5:1) was performed to obtain the 2-hydroxydodecyl-2-hexyldecanoate (1.9 g, 85% yield). ¹H NMR (400 MHz, CDCl₃): δ 0.88 (t, 9H), 1.26-1.45 (m, 36H), 1.47 (m, 2H), 1.63 (m, 2H), 2.02 (m, 1H), 2.34 (t, 2H), 3.82 (m, 1H), 3.95 (m, 1H), 4.13 (m, 1H). ESI-MS calculated for C₂₈H₅₆O₃⁺ [M+H]⁺ 441.4, found 441.5.

Embodiment 5: 2-((4-(dimethylamino)butanoyl)oxy)dodecyl 2-hexyldecanoate

EDC·HCl (192 mg, 1 mmol), DIPEA (174 μL, 1 mmol), DMAP (3.0 mg, 0.025 mmol), 4-(dimethylamino)butanoic acid (101 mg, 0.6 mmol), 2-hydroxydodecyl-2-hexyldecanoate (220 mg, 0.5 mmol) and DCM (4 mL) were sequentially added into a 10 mL reaction tube, and the reaction was stirred at room temperature for 3 h to obtain the compound E7C71A9 (235 mg, 85% yield). ¹H NMR (400 MHz, CDCl₃): δ 0.88 (t, 9H), 1.25-1.45 (m, 38H), 1.58 (m, 4H), 1.79 (m, 2H), 2.12-2.30 (m, 11H), 4.01 (m, 1H), 4.22 (m, 1H), 5.07 (m, 1H) (FIG. 1). ¹³C NMR (100 MHz, CDCl₃): δ 14.04, 14.07, 22.59, 22.64, 22.95, 25.11, 27.39, 27.43, 29.23, 29.27, 29.29, 29.45, 29.53, 29.59, 30.79, 31.68, 31.85, 31.87, 32.18, 32.37, 45.3945.69, 58.84, 64.48, 71.51, 172.98, 176.23 (FIG. 2). ESI-MS calculated for C₃₄H₆₇NO₄⁺ [M+H]⁺ 554.5, found 554.6.

Embodiment 6: 2-hydroxydecyl-octadec-9-enoate

FeCl₃ (20 mg, 0.025 mmol), Py (5 μL, 0.0125 mmol), oleic acid (1.6 mL, 5 mmol) and 1,2-epoxydecane (1.1 mL, 6 mmol) are sequentially added into a 25 mL reaction tube, and the reaction was stirred at room temperature overnight. Then column chromatography gradient elution purification (hexane:EA=20:1 to 5:1) was performed to obtain 2-hydroxydecyl-octadec-9-enoate (1.9 g, 85% yield). ¹H NMR (400 MHz, CDCl₃): δ 0.88 (t, 6H), 1.26-1.45 (m, 34H), 1.63 (m, 2H), 2.17 (m, 4H), 2.33 (m, 2H), 4.09-4.35 (m, 3H), 5.35-5.43 (m, 2H). ESI-MS calculated for C₂₈H₅₅O₃⁺ [M+H]⁺ 439.4, found 439.6.

Embodiment 7: 1-(octadec-9-enoyloxy)decan-2-yl 1-methylpiperidine-4-carboxylate

EDC·HCl (192 mg, 1 mmol), DIPEA (174 μL, 1 mmol), DMAP (3.0 mg, 0.025 mmol), 1-methylpiperidine-4-carboxylic acid (86 mg, 0.6 mmol), 2-hydroxydecyl-octadec-9-enoate (219 mg, 0.5 mmol) and DCM (4 mL) were sequentially added into a 10 mL reaction tube. The reaction was stirred at room temperature for 3 h to obtain the compound E5C82A23 (226 mg, 80% yield). ¹H NMR (400 MHz, CDCl₃): δ 0.88 (t, 6H), 1.23-1.46 (m, 32H), 1.49 (m, 2H), 1.66 (m, 2H), 1.73-2.03 (m, 4H), 2.11-2.20 (m, 7H), 2.33-2.51 (m, 7H), 4.03 (m, 1H), 4.24 (m, 1H), 5.07 (m, 1H), 5.43 (m, 2H). ¹³C NMR (100 MHz, CDCl₃): δ 14.04, 14.06, 22.59, 22.64, 25.01, 25.26, 29.23, 29.25, 29.27, 29.29, 29.45, 29.53, 29.59, 30.80, 31.65, 31.85, 31.90, 32.16, 32.39, 47.39, 47.69, 58.86, 64.49, 71.53, 130.57, 130.63, 171.97, 173.73. ESI-MS calculated for C₃₅H₆₆NO₄⁺ [M+H]⁺ 564.5, found 564.6.

Embodiment 8: 3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluoro-1-hydroxyoctyl tetradecanoate

FeCl₃ (20 mg, 0.025 mmol), Py (5 μL, 0.0125 mmol), myristic acid (1.1 g, 5 mmol) and 3-(perfluoro-n-hexyl) propenoxide (1.4 mL, 6 mmol) were sequentially added into a 25 mL reaction tube, and the reaction was stirred at room temperature overnight. Then column chromatography gradient elution purification (hexane:EA=20:1 to 5:1) was performed to obtain 3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluoro-1-hydroxyoctyl tetradecanoate (2.7 g, 90% yield). ¹H NMR (400 MHz, CDCl₃): δ 0.88 (t, 3H), 1.26-1.45 (m, 20H), 1.63 (m, 2H), 2.03 (m, 2H), 2.36 (t, 2H), 6.68 (t, 1H). ESI-MS calculated for C₂₂H₃₂F₁₃O₃⁺ [M+H]⁺ 591.2, found 591.3.

Embodiment 9: 1-((4-(dimethylamino)butyryl)oxy)-3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluorooctyl tetradecanoate

EDC·HCl (192 mg, 1 mmol), DIPEA (174 μL, 1 mmol), DMAP (3.0 mg, 0.025 mmol), 4-(dimethylamino)butanoic acid (101 mg, 0.6 mmol), 3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluoro-1-hydroxyoctyl tetradecanooate (295 mg, 0.5 mmol) and DCM (4 mL) were sequentially added into a 10 mL reaction tube, and the reaction was stirred at room temperature for 3 h to obtain the compound E24C9A9 (263.8 mg, 75% yield). ¹H NMR (400 MHz, CDCl₃): δ 0.88 (t, 3H), 1.23-1.60 (m, 20H), 1.66 (m, 2H), 1.88 (m, 2H), 2.05-2.16 (m, 8H), 2.36 (m, 4H), 3.10 (t, 2H), 7.46 (t, 1H). ¹³C NMR (100 MHz, CDCl₃): δ 14.07, 22.69, 22.95, 25.06, 27.39, 29.25, 29.41, 29.57, 29.59, 30.79, 31.85, 31.87, 32.18, 32.37, 46.39, 46.69, 59.84, 88.37, 109.05, 110.09, 111.89, 112.37, 118.49, 173.67, 176.23. ESI-MS calculated for C₂₈H₄₃F₁₃NO₄⁺ [M+H]⁺ 704.3, found 704.5.

Embodiment 10: 8-ethyl-2-hydroxydecyl palmitate

FeCl₃ (20 mg, 0.025 mmol), Py (5 μL, 0.0125 mmol), 2-hexyldecanoic acid (1.3 g, 5 mmol) and 2-(6-ethyl octyl)oxirane (1.3 mL, 6 mmol) were sequentially added into a 25 mL reaction tube, and the reaction was stirred at room temperature overnight. Then the column chromatography gradient elution purification (hexane:EA=20:1 to 5:1) was performed to obtain 8-ethyl-2-hydroxydecyl palmitate (2.0 g, 90% yield). ¹H NMR (400 MHz, CDCl₃): δ 0.88 (t, 9H), 1.26-1.45 (m, 37H), 1.47 (m, 2H), 1.63 (m, 2H), 2.02 (m, 1H), 2.34 (t, 2H), 3.82 (m, 1H), 3.95 (m, 1H), 4.13 (m, 1H). ESI-MS calculated for C₂₈H₅₇O₃⁺ [M+H]⁺ 441.4, found 441.6.

Embodiment 11: 2-((4-(dimethylamino) butyryl)oxy)-8-ethyldecyl palmitate

EDC·HCl (192 mg, 1 mmol), DIPEA (174 μL, 1 mmol), DMAP (3.0 mg, 0.025 mmol), 4-(dimethylamino)butanoic acid (101 mg, 0.6 mmol), 8-ethyl-2-hydroxydecyl palmitate (220 mg, 0.5 mmol) and DCM (4 mL) were sequentially added into a 10 mL reaction tube, and the reaction was stirred at room temperature for 3 h to obtain the compound E20C11A9 (221 mg, 80%). ¹H NMR (400 MHz, CDCl₃): δ 0.88 (t, 9H), 1.25-1.45 (m, 37H), 1.58 (m, 4H), 1.78 (m, 2H), 2.23 (s, 6H), 2.30 (m, 6H), 4.01 (m, 1H), 4.21 (m, 1H), 5.08 (m, 1H). ¹³C NMR (100 MHz, CDCl₃): δ 12.01, 12.08, 14.09, 22.59, 22.64, 22.95, 25.11, 27.39, 27.43, 29.23, 29.25, 29.31, 29.44, 29.56, 29.59, 30.80, 31.64, 31.83, 31.87, 32.21, 32.33, 46.48, 46.89, 59.45, 65.36, 71.70, 173.48, 176.73. ESI-MS calculated for C₃₄H₆₈NO₄⁺[M+H]⁺ 554.5, found 554.7.

Embodiment 12: In-Vitro Evaluation of Amino Lipid Compound as mRNA Vector

• • Cell line: HeLa cell line (ATCC)
  • Culture medium: 1640 (Invitrogen) supplemented with 10% fetal calf serum
  • Screening form: 96-well plate cell transfection
  • Detection: fluorescence intensity detection by a multifunctional microplate reader. According to manufacturer's instructions, Lipofectamine3000 (Invitrogen) was used as a positive control group.
  • Method: an 8-channel pipette was used for sample addition. The shown content is the content of a single well of a 96-well plate.
  • 1. Synthesis was performed with reference to the route described in Embodiment 1 to obtain a series of amino lipid compounds. The amino lipid compounds were mixed with DSPC, cholesterol and PEG2000-DMG according to a mole ratio of 50:10:38.5:1.5 in absolute ethyl alcohol. The Luc-mRNA (TriLink) was dissolved into a sodium acetate buffer solution (25 nM, pH=5.0). The mixed lipid solution was taken out by a multi-channel pipette tip, and was added into the Luc-mRNA solution to be sufficiently mixed. A proportion ratio of the ethyl alcohol solution to the sodium acetate buffer solution (25 nM, pH=5.0) was controlled to be 1:3, and a nanoparticle solution was prepared. A mass ratio of the amino lipid compound to luciferase mRNA (Luc mRNA) was about 10:1, and the mRNA consumption in each well was 100 ng.
  • 2. After the lipid nanoparticle solution was incubated for 30 min at room temperature, 100 μL of fresh resuspended HeLa cells (1×10⁴ cells) were added into each well of a 96-well all-white ELISA plate. Then, the lipid nanoparticle solution was added into the 96-well plate (10 μL for each well) by a pipette. The solution was placed into a 37° C. incubator containing 5% CO₂ to be incubated.
  • 3. After 16 h to 20 h of cell initial transfection, a substrate ONE-Glo™ Luciferase was added into cells at 100 μL/well, and after 2 min, detection was performed by a multifunctional microplate reader (Biorek SynergyH1).
  • 4. The relative transfection efficiency was calculated as follows:

Relative transfection efficiency (%)=fluorescence intensity of LNP/fluorescence intensity of Lip3000×100%.

Result: the transfection efficiency of parts of compounds on Luc-mRNA of the HeLa cells is shown in Table 2.

TABLE 2
relative transfection efficiency of 4345 kinds
of compounds on Luc-mRNA of the HeLa cells
	A9	A10	A11	A12	A14	A15	A16	A23	A24	A28	A33
E3C10	0.2	0.4	0.4	0.1	0.1	0.1	0.2	0.1	0.2	0.1	0.1
E3C11	0.1	0.2	0.4	0.2	0.3	0.1	0.2	0.3	0.2	0.3	0.1
E3C12	0.2	0.2	0.6	0.2	0.2	0.4	0.2	0.6	0.2	0.4	0.4
E3C13	0.5	0.8	1.9	1.2	0.6	0.2	0.4	0.7	0.4	0.6	0.2
E3C14	1.0	1.3	3.3	1.4	0.6	0.4	0.2	0.8	0.6	0.4	0.6
E3C56	0.4	0.3	0.6	0.4	0.2	0.2	0.4	0.6	0.4	0.6	0.2
E3C57	0.2	0.7	0.2	0.5	0.6	0.2	0.6	0.2	0.4	0.3	0.2
E3C58	0.7	1.0	0.7	0.2	0.6	0.2	0.4	0.6	0.2	0.6	0.9
E3C60	0.0	0.2	0.7	0.2	0.2	0.6	0.7	0.4	0.4	0.2	0.2
E3C62	0.0	0.0	0.5	0.0	0.6	0.2	0.4	0.6	0.3	0.6	0.6
E3C63	0.0	0.0	0.2	0.0	0.6	0.2	0.4	0.8	0.4	0.6	0.4
E3C64	0.2	0.2	0.7	0.2	0.6	0.2	0.2	0.3	0.6	0.2	0.4
E3C66	0.2	0.2	1.0	0.5	0.4	0.4	0.7	0.6	0.4	0.6	0.8
E3C67	0.2	0.7	1.0	0.5	0.6	0.2	0.4	0.6	0.4	0.5	0.4
E3C71	0.0	0.5	0.5	0.0	0.6	0.5	0.9	0.8	0.8	0.7	0.4
E3C72	0.5	0.2	0.7	0.2	0.6	0.5	0.6	0.6	0.4	0.8	0.4
E3C74	0.7	1.0	0.2	0.5	0.8	0.2	0.4	0.7	0.8	0.6	0.7
E3C79	0.8	1.2	4.6	3.8	0.6	0.5	0.4	0.6	0.4	0.6	0.2
E3C82	0.1	0.3	0.3	0.1	0.3	0.3	0.2	0.3	0.2	0.3	0.1
E3C83	0.1	0.1	0.3	0.2	0.1	0.1	0.5	0.7	0.7	0.3	0.1
E4C10	0.1	0.2	0.2	0.1	0.1	0.1	0.2	0.1	0.2	0.1	0.1
E4C11	0.1	0.2	0.4	0.2	0.3	0.1	0.2	0.3	0.2	0.3	0.1
E4C12	0.3	1.0	1.5	0.9	0.1	0.1	0.4	0.1	0.4	0.1	0.1
E4C13	0.7	1.7	2.7	1.2	0.3	0.6	0.2	0.3	0.2	0.3	0.1
E4C14	0.3	0.4	0.7	0.3	0.1	0.2	0.1	0.3	0.1	0.2	0.2
E4C56	0.6	0.4	0.8	0.6	0.3	0.1	0.2	0.8	0.2	0.3	0.1
E4C57	0.2	0.6	0.2	0.4	0.3	0.2	0.1	0.4	0.3	0.2	0.3
E4C58	0.6	0.8	0.6	0.5	0.3	0.7	0.5	0.3	0.2	0.3	0.1
E4C60	0.0	0.2	0.6	0.5	0.8	0.3	0.8	0.1	0.2	0.5	0.2
E4C62	0.0	0.0	0.4	0.0	0.8	0.3	0.5	0.3	0.6	0.6	0.2
E4C63	0.0	0.0	0.2	0.0	0.3	0.8	0.5	0.2	0.2	0.2	0.2
E4C64	0.2	0.2	0.6	0.2	0.3	0.1	0.2	0.3	0.2	0.7	0.6
E4C66	0.2	0.2	0.8	0.4	0.3	0.1	0.2	0.4	0.2	0.6	0.2
E4C67	0.2	0.6	0.8	0.4	0.3	0.1	0.6	0.2	0.3	0.2	0.2
E4C71	0.0	0.4	0.4	0.0	0.2	0.6	0.2	0.3	0.2	0.9	0.2
E4C72	0.8	0.4	1.2	0.4	0.3	0.1	0.2	0.2	0.2	0.3	0.8
E4C74	2.4	3.7	5.1	4.3	0.3	0.1	0.2	0.4	0.6	0.3	0.1
E4C79	2.9	4.5	6.1	5.1	0.6	0.1	0.3	0.3	0.1	0.6	0.1
E4C82	0.1	0.3	0.3	0.1	0.2	0.1	0.1	0.4	0.1	0.2	0.1
E4C83	0.1	0.1	0.3	0.2	0.2	0.1	0.1	0.2	0.1	0.2	0.1
E5C10	0.2	0.4	0.4	0.1	0.1	0.1	0.4	0.1	0.4	0.1	0.1
E5C11	0.8	1.0	2.9	1.3	0.3	0.1	0.2	0.3	0.2	0.3	0.1
E5C12	0.5	0.7	1.8	1.1	0.1	0.1	0.4	0.1	0.4	0.1	0.1
E5C13	0.9	1.2	3.3	1.4	0.3	0.7	0.2	0.3	0.2	0.3	0.4
E5C14	0.2	0.3	0.8	0.4	0.1	0.2	0.1	0.3	0.1	0.6	0.6
E5C56	0.7	0.4	0.9	0.7	0.3	0.1	0.2	0.9	0.2	0.9	0.3
E5C57	0.2	0.7	0.2	0.4	0.3	0.2	0.1	0.4	0.3	0.6	0.9
E5C58	0.7	0.9	0.7	0.2	0.1	0.4	0.2	0.3	0.2	0.9	0.3
E5C60	0.0	0.2	0.7	0.2	0.3	0.1	0.3	0.1	0.2	0.3	0.3
E5C62	0.0	0.0	0.4	0.0	0.3	0.1	0.2	0.3	0.7	0.9	0.3
E5C63	0.0	0.0	0.2	0.0	0.1	0.3	0.2	0.2	0.2	0.3	0.3
E5C64	0.2	0.2	0.7	0.2	0.8	0.1	0.2	0.3	0.2	0.9	0.9
E5C66	0.2	0.2	0.5	0.4	0.3	0.4	0.2	0.4	0.2	0.3	0.1
E5C67	0.2	0.7	0.5	0.4	0.3	0.1	0.7	0.2	0.3	0.1	0.1
E5C71	0.6	0.9	4.0	0.6	0.2	0.7	0.2	0.3	0.2	0.3	0.1
E5C72	0.5	0.8	3.6	0.6	0.3	0.1	0.2	0.2	0.2	0.3	0.9
E5C74	0.6	3.2	4.3	0.7	0.3	0.1	0.2	0.4	0.7	0.3	0.1
E5C79	0.8	3.8	5.2	0.8	0.7	0.1	0.3	0.3	0.1	0.7	0.1
E5C82	0.2	0.7	0.7	0.2	0.4	0.4	0.2	0.9	0.2	0.3	0.6
E5C83	0.1	0.1	0.3	0.2	0.2	0.1	0.1	0.2	0.1	0.2	0.1
E6C9	0.2	0.4	0.4	0.1	0.1	0.1	0.4	0.1	0.4	0.1	0.1
E6C10	0.9	1.9	1.4	0.6	0.2	0.5	0.1	0.2	0.1	0.2	0.1
E6C11	0.8	1.7	1.3	0.9	0.2	0.1	0.1	0.2	0.1	0.2	0.1
E6C12	0.2	0.6	0.8	0.8	0.1	0.7	0.3	0.1	0.3	0.1	0.1
E6C13	0.5	0.9	1.4	1.0	0.5	0.6	0.3	0.2	0.1	0.2	0.2
E6C14	0.6	0.8	1.2	0.5	0.2	0.3	0.2	0.2	0.1	0.1	0.1
E6C56	0.4	0.3	0.5	0.4	0.5	0.2	0.3	0.5	0.1	0.2	0.1
E6C57	0.1	0.4	0.1	0.3	0.5	0.3	0.2	0.3	0.2	0.1	0.2
E6C58	0.4	0.5	0.4	0.1	0.2	0.7	0.3	0.2	0.1	0.2	0.1
E6C60	0.0	0.1	0.4	0.1	0.5	0.2	0.5	0.1	0.1	0.2	0.1
E6C62	0.0	0.0	0.3	0.0	0.2	0.1	0.8	0.2	0.4	0.2	0.6
E6C63	0.0	0.0	0.1	0.0	0.1	0.2	0.1	0.1	0.1	0.1	0.1
E6C64	0.1	0.1	0.4	0.1	0.2	0.1	0.1	0.2	0.1	0.2	0.2
E6C66	0.1	0.1	0.5	0.3	0.2	0.1	0.1	0.3	0.4	0.2	0.1
E6C67	1.2	1.8	7.1	6.0	0.2	0.1	0.4	0.8	0.2	0.1	0.5
E6C71	2.6	0.9	3.5	3.0	0.1	0.8	0.3	0.4	0.3	0.4	0.1
E6C72	0.5	0.3	3.2	0.2	0.6	0.1	0.3	0.3	0.3	0.4	0.9
E6C74	0.6	2.8	3.8	1.1	0.2	0.1	0.3	0.7	0.8	0.4	0.1
E6C79	0.8	3.4	4.6	1.3	0.4	0.1	0.4	0.4	0.1	0.8	0.1
E6C82	0.1	0.4	0.4	0.1	0.3	0.5	0.3	0.8	0.3	0.4	0.7
E6C83	0.1	0.1	0.4	0.3	0.2	0.1	0.3	0.4	0.3	0.4	0.1
E7C8	0.2	0.4	0.4	0.1	0.1	0.1	0.4	0.1	0.4	0.1	0.1
E7C9	1.2	0.9	1.4	1.0	0.1	0.1	0.6	0.1	0.6	0.1	0.1
E7C10	0.7	0.5	0.8	0.6	0.4	0.4	0.2	0.4	0.8	0.4	0.4
E7C11	1.1	0.8	1.2	0.9	0.3	0.6	0.2	0.3	0.2	0.3	0.6
E7C12	0.6	0.3	0.8	0.8	0.4	0.2	0.3	0.2	0.3	0.6	0.1
E7C13	0.4	0.9	1.4	1.0	0.3	0.5	0.2	0.3	0.2	0.3	0.3
E7C14	0.6	0.4	0.7	0.5	0.6	0.2	0.1	0.3	0.1	0.2	0.2
E7C56	0.5	0.3	0.7	0.5	0.3	0.1	0.2	0.7	0.2	0.3	0.1
E7C57	0.2	0.5	0.2	0.3	0.3	0.2	0.1	0.3	0.3	0.6	0.3
E7C58	0.5	0.7	0.5	0.2	0.6	0.3	0.2	0.3	0.2	0.3	0.6
E7C60	0.0	0.2	0.5	0.2	0.3	0.2	0.3	0.6	0.2	0.3	0.4
E7C62	0.0	0.0	0.3	0.0	0.3	0.6	0.8	0.3	0.5	0.3	0.8
E7C63	0.0	0.0	0.2	0.0	0.4	0.3	0.2	0.2	0.2	0.4	0.1
E7C64	0.2	0.2	0.5	0.2	0.3	0.4	0.2	0.3	0.8	0.3	0.3
E7C66	0.2	0.2	0.7	0.3	0.3	0.1	0.2	0.3	0.5	0.3	0.1
E7C67	1.1	5.6	5.3	1.4	0.8	0.1	0.5	0.8	0.3	0.1	0.6
E7C71	1.5	2.8	2.7	0.7	0.2	0.5	0.2	0.3	0.4	0.3	0.2
E7C72	0.5	0.8	0.8	0.6	0.8	0.1	0.7	0.2	0.2	0.8	0.7
E7C74	2.0	3.0	2.9	2.4	0.3	0.1	0.2	0.8	0.5	0.3	0.1
E7C79	2.3	3.7	3.5	2.9	0.5	0.1	0.3	0.3	0.1	0.5	0.2
E7C82	0.2	0.5	0.5	0.2	0.3	0.3	0.2	0.7	0.2	0.3	0.5
E7C83	0.2	0.2	0.5	0.3	0.3	0.1	0.9	0.3	0.6	0.3	0.1
E8C7	0.1	0.1	0.3	0.2	0.1	0.0	0.1	0.1	0.3	0.1	0.0
E8C8	0.2	0.3	0.3	0.2	0.2	0.1	0.1	0.2	0.1	0.2	0.1
E8C9	0.3	0.2	0.4	0.3	0.1	0.1	0.6	0.1	0.6	0.1	0.1
E8C10	0.5	0.4	0.6	0.4	0.4	0.4	0.2	0.4	0.8	0.4	0.4
E8C11	0.8	0.6	0.9	0.7	0.3	0.6	0.2	0.3	0.2	0.3	0.6
E8C12	0.4	0.4	0.6	0.6	0.4	0.2	0.3	0.6	0.3	0.6	0.1
E8C13	0.9	0.6	1.0	0.7	0.4	0.7	0.2	0.4	0.2	0.4	0.4
E8C14	3.9	2.9	4.4	3.3	0.8	0.2	0.1	0.4	0.3	0.2	0.2
E8C56	0.5	0.3	0.7	0.5	0.4	0.1	0.4	0.6	0.2	0.4	0.3
E8C57	0.2	0.5	0.2	0.3	0.4	0.2	0.1	0.5	0.6	0.8	0.4
E8C58	0.5	0.7	0.5	0.2	0.8	0.5	0.2	0.4	0.2	0.4	0.8
E8C60	0.0	0.2	0.5	0.2	0.4	0.3	0.4	0.8	0.2	0.4	0.6
E8C62	0.5	0.8	0.8	0.6	0.4	0.8	0.6	0.4	0.7	0.4	0.2
E8C63	0.7	0.6	0.6	0.8	0.6	0.4	0.2	0.2	0.2	0.6	0.8
E8C64	0.7	0.7	0.6	0.9	0.4	0.6	0.2	0.4	0.4	0.4	0.4
E8C66	0.7	0.6	0.6	0.8	0.4	0.1	0.2	0.5	0.7	0.4	0.6
E8C67	0.5	0.5	0.8	0.6	0.3	0.1	0.7	0.6	0.4	0.1	0.8
E8C71	3.6	0.6	3.1	4.5	0.1	0.7	0.2	0.4	0.6	0.4	0.3
E8C72	3.3	0.5	2.8	4.1	0.7	0.1	0.2	0.2	0.2	0.4	0.4
E8C74	3.9	0.7	3.3	4.9	0.2	0.1	0.2	0.6	0.7	0.4	0.3
E8C79	4.7	3.8	6.0	5.8	0.4	0.1	0.4	0.4	0.1	0.7	0.3
E8C82	0.2	0.5	0.5	0.2	0.5	0.4	0.2	0.2	0.2	0.4	0.6
E8C83	0.2	0.2	0.5	0.3	0.4	0.1	0.2	0.4	0.8	0.4	0.3
E9C3	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0
E9C4	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0
E9C5	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0
E9C6	0.1	0.2	0.2	0.1	0.1	0.1	0.2	0.1	0.2	0.1	0.1
E9C7	0.2	0.3	0.3	0.3	0.2	0.2	0.1	0.2	0.4	0.2	0.2
E9C8	0.6	0.9	0.8	0.7	0.1	0.1	0.4	0.1	0.4	0.1	0.1
E9C9	0.5	0.8	0.8	0.6	0.1	0.1	0.6	0.1	0.6	0.1	0.1
E9C10	0.6	0.6	0.4	0.8	0.4	0.4	0.2	0.4	0.8	0.4	0.4
E9C11	0.5	0.8	0.7	0.6	0.3	0.6	0.2	0.3	0.2	0.3	0.6
E9C12	0.8	0.6	0.4	0.7	0.4	0.2	0.3	0.2	0.3	0.6	0.1
E9C13	0.5	0.6	0.5	0.5	0.3	0.5	0.2	0.3	0.2	0.3	0.3
E9C14	0.6	3.0	0.5	0.4	0.6	0.2	0.1	0.3	0.1	0.2	0.2
E9C56	0.7	3.6	0.6	0.5	0.3	0.1	0.2	0.7	0.2	0.3	0.1
E9C57	0.9	4.3	0.8	0.6	0.3	0.2	0.1	0.3	0.3	0.6	0.3
E9C58	1.3	6.6	1.2	1.0	0.6	0.3	0.2	0.3	0.2	0.3	0.6
E9C60	0.8	4.1	0.7	0.6	0.3	0.2	0.3	0.6	0.2	0.3	0.4
E9C62	0.8	3.8	0.7	0.6	0.3	0.6	0.8	0.3	0.5	0.3	0.8
E9C63	0.9	4.5	0.8	0.7	0.4	0.3	0.2	0.2	0.2	0.4	0.1
E9C64	1.1	5.4	1.0	0.8	0.3	0.4	0.2	0.3	0.8	0.3	0.3
E9C66	1.7	8.3	1.5	1.2	0.3	0.1	0.2	0.3	0.5	0.3	0.1
E9C67	0.8	4.1	0.7	0.6	0.8	0.1	0.5	0.8	0.3	0.1	0.6
E9C71	0.8	3.8	0.7	0.6	0.2	0.5	0.2	0.3	0.4	0.3	0.2
E9C72	0.9	4.5	0.8	0.7	0.8	0.1	0.2	0.2	0.2	0.3	0.7
E9C74	1.1	5.4	3.1	0.8	0.3	0.1	0.2	0.8	0.5	0.3	0.1
E9C79	0.6	3.2	1.8	0.5	0.5	0.1	0.3	0.3	0.1	0.5	0.2
E9C82	0.2	0.5	0.5	0.2	0.3	0.3	0.2	0.7	0.2	0.3	0.5
E9C83	0.2	0.2	0.5	0.3	0.3	0.1	0.2	0.3	0.6	0.3	0.1
E10C3	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0
E10C4	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0
E10C5	0.2	0.1	0.1	0.1	0.1	0.2	0.0	0.1	0.1	0.2	0.0
E10C6	0.2	0.2	0.2	0.1	0.3	0.2	0.1	0.1	0.1	0.1	0.3
E10C7	0.2	0.3	0.2	0.2	0.1	0.1	0.1	0.3	0.1	0.1	0.2
E10C8	0.7	0.8	0.6	0.5	0.3	0.6	0.8	0.3	0.5	0.3	0.8
E10C9	0.4	0.4	0.3	0.3	0.4	0.3	0.2	0.2	0.2	0.4	0.1
E10C10	0.5	0.4	0.6	0.4	0.4	0.4	0.2	0.4	0.8	0.4	0.4
E10C11	0.8	0.6	0.9	0.7	0.3	0.6	0.2	0.3	0.2	0.3	0.6
E10C12	0.4	0.4	0.6	0.6	0.4	0.2	0.3	0.6	0.3	0.6	0.1
E10C13	0.9	0.6	0.6	0.7	0.4	0.7	0.2	0.4	0.2	0.4	0.4
E10C14	3.9	2.9	4.4	3.3	0.8	0.2	0.1	0.4	0.3	0.2	0.2
E10C56	0.5	0.3	0.7	0.5	0.4	0.1	0.4	0.6	0.2	0.4	0.3
E10C57	0.2	0.5	0.2	0.3	0.4	0.2	0.1	0.5	0.6	0.8	0.4
E10C58	0.5	0.7	0.5	0.2	0.8	0.5	0.2	0.4	0.2	0.4	0.8
E10C60	0.0	0.2	0.5	0.2	0.4	0.3	0.4	0.8	0.2	0.4	0.6
E10C62	0.5	0.8	0.8	0.6	0.4	0.8	0.6	0.4	0.7	0.4	0.2
E10C63	0.7	0.6	0.6	0.8	0.6	0.4	0.2	0.2	0.2	0.6	0.8
E10C64	0.7	0.7	0.6	0.6	0.4	0.6	0.2	0.4	0.4	0.4	0.4
E10C66	0.7	0.6	0.6	0.8	0.4	0.1	0.2	0.5	0.7	0.4	0.6
E10C67	0.5	0.5	0.8	0.6	0.3	0.1	0.7	0.6	0.4	0.1	0.8
E10C71	0.6	0.4	1.8	1.2	0.1	0.7	0.2	0.4	0.6	0.4	0.3
E10C72	1.9	0.3	1.6	0.8	0.7	0.1	0.2	0.2	0.2	0.4	0.4
E10C74	2.3	1.3	2.0	0.6	0.2	0.1	0.2	0.6	0.7	0.4	0.3
E10C79	2.8	2.3	3.6	1.5	0.4	0.1	0.4	0.4	0.1	0.7	0.3
E10C82	0.2	0.5	0.5	0.2	0.5	0.4	0.2	0.2	0.2	0.4	0.6
E10C83	0.2	0.2	0.5	0.3	0.4	0.1	0.2	0.4	0.8	0.4	0.3
E11C6	0.2	0.4	0.4	0.1	0.1	0.1	0.4	0.1	0.4	0.1	0.1
E11C7	1.9	2.4	12.8	2.0	0.8	0.4	0.3	0.8	0.9	0.8	0.8
E11C8	0.6	0.9	0.8	0.7	0.1	0.1	0.4	0.1	0.4	0.1	0.1
E11C9	0.5	0.8	0.8	0.6	0.1	0.1	0.6	0.1	0.6	0.1	0.1
E11C10	0.6	0.6	0.4	0.8	0.4	0.4	0.2	0.4	0.8	0.4	0.4
E11C11	0.5	0.8	0.7	0.6	0.3	0.6	0.2	0.3	0.2	0.3	0.6
E11C12	0.8	0.6	0.4	0.7	0.4	0.2	0.3	0.2	0.3	0.6	0.1
E11C13	0.5	0.6	0.5	0.5	0.3	0.5	0.2	0.3	0.2	0.3	0.3
E11C14	0.6	0.8	0.5	0.4	0.6	0.2	0.1	0.3	0.1	0.2	0.2
E11C56	0.7	0.4	0.6	0.5	0.3	0.1	0.2	0.7	0.2	0.3	0.1
E11C57	0.6	0.7	4.3	0.6	0.3	0.2	0.1	0.3	0.3	0.6	0.3
E11C58	0.9	1.2	6.6	1.0	0.6	0.3	0.2	0.3	0.2	0.3	0.6
E11C60	0.6	0.7	4.1	0.6	0.3	0.2	0.3	0.6	0.2	0.3	0.4
E11C62	0.5	0.7	3.8	0.6	0.3	0.6	0.8	0.3	0.5	0.3	0.8
E11C63	0.4	0.8	0.6	0.5	0.4	0.3	0.2	0.2	0.2	0.4	0.1
E11C64	0.8	1.4	0.7	0.6	0.3	0.4	0.2	0.3	0.8	0.3	0.3
E11C66	1.2	2.1	1.0	0.9	0.3	0.1	0.2	0.3	0.5	0.3	0.1
E11C67	1.0	0.5	0.5	0.4	0.8	0.1	0.5	0.8	0.3	0.1	0.6
E11C71	1.4	0.7	0.7	0.6	0.2	0.5	0.2	0.3	0.4	0.3	0.2
E11C72	1.6	0.8	0.8	0.7	0.8	0.1	0.2	0.2	0.2	0.3	0.7
E11C74	1.4	0.7	2.1	0.6	0.3	0.1	0.2	0.8	0.5	0.3	0.1
E11C79	1.4	0.7	2.1	0.6	0.5	0.1	0.3	0.3	0.1	0.5	0.2
E11C82	0.2	0.5	0.5	0.2	0.3	0.3	0.2	0.7	0.2	0.3	0.5
E11C83	0.2	0.2	0.5	0.3	0.3	0.1	0.2	0.3	0.6	0.3	0.1
E12C3	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0
E12C4	0.2	0.2	0.1	0.3	0.1	0.1	0.1	0.1	0.3	0.2	0.2
E12C5	0.2	0.3	0.3	0.2	0.1	0.2	0.1	0.1	0.1	0.1	0.3
E12C6	0.2	1.4	4.4	0.1	0.1	0.1	0.3	0.1	0.3	0.1	0.1
E12C7	0.6	0.4	0.6	0.7	0.6	0.3	0.2	0.6	0.8	0.8	0.4
E12C8	0.4	0.6	0.6	0.5	0.1	0.1	0.3	0.1	0.3	0.1	0.1
E12C9	0.4	0.6	0.5	0.4	0.1	0.1	0.4	0.1	0.4	0.1	0.1
E12C10	0.4	0.4	0.3	0.5	0.3	0.3	0.1	0.3	0.6	0.4	0.2
E12C11	0.4	0.5	0.5	0.4	0.2	0.4	0.1	0.2	0.1	0.3	0.3
E12C12	0.5	0.4	0.3	0.5	0.3	0.1	0.2	0.1	0.2	0.6	0.0
E12C13	0.4	0.4	0.4	0.3	0.2	0.4	0.1	0.2	0.1	0.3	0.2
E12C14	0.4	0.6	0.4	0.2	0.4	0.1	0.1	0.2	0.1	0.2	0.1
E12C56	0.5	0.3	0.4	0.4	0.2	0.1	0.1	0.5	0.1	0.3	0.0
E12C57	0.4	0.5	0.7	0.4	0.2	0.1	0.1	0.2	0.2	0.6	0.1
E12C58	0.6	0.8	0.6	0.7	0.4	0.2	0.1	0.2	0.1	0.3	0.3
E12C60	0.4	0.5	0.7	0.4	0.2	0.1	0.2	0.4	0.1	0.3	0.2
E12C62	0.4	0.5	0.7	0.4	0.2	0.4	0.6	0.2	0.4	0.3	0.4
E12C63	0.3	0.6	0.4	0.3	0.3	0.2	0.1	0.1	0.1	0.4	0.0
E12C64	0.5	1.9	0.5	0.4	0.2	0.3	0.1	0.2	0.6	0.3	0.1
E12C66	0.8	2.9	0.7	0.6	0.2	0.1	0.1	0.2	0.4	0.3	0.0
E12C67	0.4	1.5	0.4	0.3	0.6	0.1	0.4	0.6	0.2	0.1	0.3
E12C71	1.9	0.9	0.5	0.4	0.1	0.4	0.1	0.2	0.3	0.3	0.1
E12C72	2.3	0.9	0.6	0.5	0.5	0.1	0.1	0.1	0.1	0.3	0.3
E12C74	1.9	0.7	1.5	0.4	0.2	0.1	0.1	0.5	0.4	0.3	0.0
E12C79	1.9	0.8	1.5	0.4	0.4	0.1	0.2	0.2	0.1	0.5	0.1
E12C82	0.1	0.3	0.3	0.1	0.2	0.2	0.1	0.3	0.1	0.1	0.2
E12C83	0.1	0.1	0.3	0.2	0.1	0.0	0.1	0.1	0.3	0.1	0.0
E13C3	0.2	0.3	0.3	0.2	0.1	0.1	0.2	0.1	0.2	0.1	0.1
E13C4	0.4	0.4	0.3	0.5	0.3	0.3	0.1	0.3	0.6	0.4	0.4
E13C5	1.4	1.4	3.8	0.4	0.2	0.4	0.1	0.2	0.1	0.3	0.6
E13C6	0.2	0.8	0.6	0.1	0.1	0.1	0.3	0.1	0.3	0.1	0.1
E13C7	0.6	0.4	0.6	0.7	0.6	0.3	0.2	0.6	0.8	0.8	0.8
E13C8	0.4	0.6	0.6	0.5	0.1	0.1	0.3	0.1	0.3	0.1	0.1
E13C9	0.4	0.6	0.5	0.4	0.2	0.2	0.8	0.2	0.8	0.3	0.3
E13C10	0.4	0.4	0.3	0.5	0.6	0.6	0.2	0.6	0.4	0.8	0.8
E13C11	0.4	0.5	0.5	0.4	0.4	0.8	0.2	0.4	0.2	0.5	0.3
E13C12	0.5	0.4	0.3	0.5	0.6	0.3	0.5	0.3	0.5	0.9	0.2
E13C13	0.4	0.4	0.4	0.3	0.4	0.7	0.2	0.4	0.2	0.5	0.6
E13C14	0.4	0.6	0.4	0.3	0.8	0.2	0.1	0.4	0.1	0.3	0.3
E13C56	0.5	0.3	0.4	0.4	0.4	0.1	0.2	0.7	0.2	0.5	0.2
E13C57	0.4	0.5	0.7	0.4	0.4	0.2	0.1	0.5	0.4	0.9	0.5
E13C58	0.6	0.8	0.6	0.7	0.8	0.5	0.2	0.4	0.2	0.5	0.6
E13C60	0.4	0.5	0.7	0.4	0.4	0.3	0.4	0.8	0.2	0.5	0.8
E13C62	0.4	2.5	0.7	0.4	0.4	0.8	0.6	0.4	0.7	0.5	0.9
E13C63	0.3	3.8	0.4	0.3	0.6	0.4	0.2	0.2	0.2	0.8	0.2
E13C64	0.5	1.9	0.5	0.4	0.4	0.6	0.2	0.4	0.8	0.5	0.5
E13C66	0.8	2.5	0.7	0.6	0.2	0.1	0.1	0.2	0.4	0.3	0.1
E13C67	0.4	2.9	3.4	0.3	0.6	0.1	0.4	0.6	0.2	0.1	0.6
E13C71	0.5	2.5	0.5	0.4	0.1	0.4	0.1	0.2	0.3	0.3	0.2
E13C72	0.6	0.8	0.6	0.5	0.5	0.1	0.5	0.7	0.1	0.3	0.7
E13C74	0.3	0.4	0.1	0.2	0.1	0.0	0.1	0.3	0.2	0.1	0.0
E13C79	0.3	0.3	0.4	0.2	0.2	0.0	0.6	0.1	0.0	0.3	0.1
E13C82	0.1	0.3	0.3	0.1	0.2	0.2	0.1	0.3	0.1	0.1	0.2
E13C83	0.1	0.1	0.3	0.2	0.1	0.0	0.1	0.1	0.3	0.1	0.0
E15C10	0.4	0.8	0.8	0.2	0.2	0.2	0.4	0.2	0.4	0.2	0.2
E15C11	0.2	0.4	0.8	0.4	0.6	0.2	0.4	0.6	0.4	0.6	0.2
E15C12	0.2	0.2	0.6	0.2	0.2	0.4	0.2	0.6	0.2	0.4	0.4
E15C13	0.5	0.8	0.4	0.8	0.7	0.2	0.4	0.6	0.4	0.6	0.2
E15C14	0.8	0.4	0.6	0.6	0.6	0.4	0.2	0.8	0.6	0.4	0.6
E15C56	0.4	0.3	0.6	0.4	0.1	0.2	0.4	0.6	0.4	0.6	0.2
E15C57	0.2	0.7	0.2	0.5	0.6	0.2	0.6	0.2	0.7	0.6	0.2
E15C58	0.7	0.6	0.7	0.2	0.6	0.2	0.7	0.6	0.2	0.6	0.2
E15C60	0.0	0.2	0.7	0.2	0.2	0.6	0.4	0.4	0.4	0.2	0.2
E15C62	0.0	0.0	0.5	0.0	0.6	0.5	0.9	0.6	0.4	0.6	0.6
E15C63	0.0	0.0	0.2	0.0	0.3	0.5	0.3	0.8	0.8	0.6	0.2
E15C64	0.2	0.2	0.7	0.2	0.6	0.2	0.2	0.6	0.6	0.2	0.2
E15C66	0.2	0.2	1.4	0.5	0.4	0.4	0.4	0.6	0.4	0.6	0.2
E15C67	0.2	0.7	1.0	0.5	0.6	0.2	0.4	0.6	0.4	0.6	0.2
E15C71	0.0	0.5	0.5	0.0	0.9	0.8	0.4	0.8	0.4	0.6	0.2
E15C72	0.5	0.2	0.7	0.2	0.6	0.2	0.6	0.6	0.2	0.8	0.2
E15C74	0.7	1.0	0.2	0.5	0.8	0.5	0.7	0.6	0.4	0.6	0.2
E15C79	0.8	1.2	4.6	3.8	0.6	0.5	0.3	0.6	0.4	0.6	0.2
E15C82	0.2	0.6	0.6	0.2	0.6	0.5	0.9	0.6	0.4	0.6	0.2
E15C83	0.2	0.2	0.6	0.4	0.2	0.2	0.4	0.6	0.4	0.6	0.2
E17C3	0.1	0.2	0.1	0.2	0.2	0.2	0.1	0.2	0.1	0.2	0.2
E17C4	0.1	0.1	0.2	0.1	0.2	0.2	0.1	0.2	0.1	0.1	0.3
E17C5	0.1	0.2	0.2	0.7	0.4	0.3	0.2	0.2	0.1	0.2	0.2
E17C6	0.2	0.2	0.2	0.6	0.2	0.5	0.1	0.1	0.2	0.1	0.2
E17C7	0.1	0.3	0.5	0.6	0.4	0.6	0.1	0.2	0.4	0.2	0.2
E17C8	0.1	0.2	0.2	0.4	0.2	0.9	0.2	0.2	0.1	0.1	0.1
E17C9	0.1	0.2	0.2	0.3	0.5	0.6	0.4	0.3	0.2	0.0	0.2
E17C10	0.3	0.2	0.3	0.4	0.2	0.4	0.2	0.4	0.2	0.1	0.4
E17C11	0.3	0.5	0.3	0.4	0.2	0.2	0.4	0.8	0.4	0.7	0.4
E17C12	0.4	0.4	0.2	0.7	0.4	0.1	0.8	0.6	0.4	0.8	0.8
E17C13	0.1	0.5	0.3	0.4	0.9	0.2	0.4	0.4	0.8	0.3	0.5
E17C14	0.4	0.3	0.2	0.4	0.2	0.8	0.4	0.7	0.9	0.7	0.3
E17C56	0.1	0.2	0.4	0.3	0.2	0.8	0.8	0.9	0.4	0.2	0.2
E17C57	0.1	0.4	0.2	0.2	0.4	0.8	0.5	0.3	0.8	0.1	0.4
E17C58	0.1	0.4	0.2	0.4	0.8	0.4	0.4	0.9	0.4	0.2	0.4
E17C60	0.4	0.2	0.4	0.2	0.4	0.4	0.4	0.8	0.4	0.1	0.4
E17C62	0.7	0.4	0.5	0.3	0.4	0.4	0.7	0.4	0.8	0.9	0.2
E17C63	0.4	0.2	0.3	0.2	0.7	0.8	0.9	0.4	0.8	0.7	0.2
E17C64	0.4	0.2	0.2	0.4	0.4	0.5	0.8	0.9	0.4	0.2	0.2
E17C66	0.6	0.4	2.2	1.2	0.4	0.4	0.2	0.4	0.8	0.1	0.4
E17C67	1.9	0.3	2.0	0.8	0.1	0.2	0.2	0.4	0.2	0.1	0.4
E17C71	2.8	1.6	2.3	0.6	0.1	0.4	0.2	0.4	0.2	0.8	0.4
E17C72	3.3	2.7	4.3	1.5	0.4	0.2	0.4	0.2	0.4	0.2	0.2
E17C74	0.4	0.4	0.8	0.1	0.7	0.4	0.5	0.3	0.4	0.2	0.2
E17C79	0.2	0.4	0.3	0.2	0.4	0.2	0.3	0.2	0.7	0.4	0.2
E17C82	0.2	0.1	0.1	0.2	0.2	0.2	0.1	0.2	0.1	0.2	0.1
E17C83	0.2	0.1	0.2	0.4	0.1	0.7	0.4	0.5	0.3	0.4	0.1
E18C3	0.1	0.1	0.2	0.1	0.1	0.3	0.4	0.6	0.4	0.6	0.1
E18C4	0.1	0.1	0.2	0.0	0.2	0.2	0.6	0.6	0.4	0.6	0.1
E18C5	0.4	0.4	0.8	0.1	0.4	0.3	0.9	0.1	0.7	0.4	0.2
E18C6	0.1	0.2	0.3	0.4	0.4	0.8	0.8	0.3	0.9	0.1	0.4
E18C7	0.4	0.5	0.3	0.2	0.3	0.2	0.3	0.4	0.4	0.1	0.4
E18C8	0.1	0.4	0.2	0.5	0.3	0.5	0.3	0.2	0.8	0.2	0.7
E18C9	0.1	0.5	0.3	0.1	0.4	0.4	0.2	0.5	0.3	0.1	0.4
E18C10	0.1	0.3	0.2	0.4	0.4	0.5	0.3	0.4	0.2	0.4	0.4
E18C11	0.2	0.2	0.4	0.2	0.3	0.3	0.2	0.5	0.3	0.3	0.5
E18C12	0.1	0.4	0.2	0.5	0.3	0.2	0.4	0.3	0.2	0.4	0.4
E18C13	0.4	0.4	0.2	0.4	0.2	0.4	0.2	0.2	0.4	0.1	0.5
E18C14	0.2	0.7	0.4	0.5	0.6	0.9	0.4	0.7	0.6	0.7	0.3
E18C56	0.2	0.4	0.2	0.3	0.4	0.3	0.8	0.9	0.4	0.2	0.2
E18C57	0.2	0.4	0.2	0.2	0.8	0.9	0.7	0.8	0.8	0.1	0.4
E18C58	0.3	0.2	0.3	0.4	0.4	0.8	0.4	0.9	0.4	0.2	0.4
E18C60	0.3	0.5	0.3	0.4	0.6	0.2	0.4	0.8	0.4	0.5	0.4
E18C62	0.4	0.4	0.2	0.7	0.8	0.1	0.8	0.6	0.4	0.8	0.8
E18C63	0.1	0.5	0.3	0.4	0.4	0.2	0.4	0.4	0.8	0.3	0.5
E18C64	0.4	0.3	0.2	0.4	0.3	0.8	0.4	0.7	0.4	0.7	0.3
E18C66	0.1	0.2	0.4	0.3	0.4	0.8	0.8	0.9	0.4	0.2	0.2
E18C67	0.2	1.5	0.7	0.2	0.4	0.4	0.8	0.7	0.4	0.1	0.4
E18C71	0.5	1.8	0.7	0.4	0.8	0.2	0.2	0.4	0.2	0.1	0.4
E18C72	1.8	0.7	1.8	0.2	0.4	0.2	0.2	0.4	0.2	0.8	0.4
E18C74	2.8	1.7	1.9	0.3	0.4	0.2	0.4	0.2	0.4	0.6	0.2
E18C79	1.7	0.7	1.3	0.2	0.9	0.8	0.9	0.4	0.8	0.6	0.4
E18C82	0.2	0.1	0.1	0.2	0.4	0.8	0.4	0.4	0.2	0.1	0.2
E18C83	0.1	0.2	0.2	0.1	0.4	0.2	0.8	0.7	0.4	0.1	0.4
E20C9	1.4	2.2	2.1	0.9	0.1	0.1	0.4	0.1	0.4	0.1	0.1
E20C10	1.7	1.7	1.1	1.1	0.3	0.3	0.1	0.3	0.6	0.4	0.4
E20C11	1.4	2.2	2.1	0.9	0.2	0.4	0.1	0.2	0.1	0.3	0.6
E20C12	2.2	1.7	1.1	0.9	0.6	0.3	0.5	0.3	0.5	0.6	0.1
E20C13	0.2	0.2	0.2	0.3	0.4	0.7	0.2	0.4	0.2	0.3	0.3
E20C14	0.4	0.6	0.4	0.3	0.8	0.2	0.1	0.4	0.1	0.2	0.2
E20C56	0.5	0.3	0.4	0.2	0.4	0.1	0.9	0.7	0.2	0.3	0.1
E20C57	0.4	0.5	0.7	0.4	0.2	0.1	0.1	0.2	0.2	0.6	0.3
E20C58	0.6	0.8	0.6	0.7	0.4	0.2	0.1	0.2	0.1	0.3	0.6
E20C60	0.4	0.5	0.7	0.9	0.4	0.3	0.4	0.8	0.2	0.4	0.4
E20C62	0.4	0.7	0.7	0.8	0.4	0.8	0.5	0.4	0.7	0.5	0.8
E20C63	2.5	0.4	0.4	0.7	0.6	0.4	0.2	0.2	0.2	0.8	0.1
E20C64	3.8	0.3	0.5	0.8	0.4	0.6	0.2	0.4	0.5	0.5	0.3
E20C66	1.9	0.7	0.7	0.5	0.4	0.1	0.2	0.5	0.7	0.5	0.1
E20C67	2.5	0.6	0.9	0.6	0.5	0.1	0.7	0.9	0.4	0.2	0.6
E20C71	2.9	0.9	0.5	0.8	0.2	0.7	0.2	0.4	0.6	0.5	0.2
E20C72	2.5	0.8	0.6	0.9	0.6	0.1	0.2	0.2	0.2	0.4	0.7
E20C74	0.3	0.4	0.1	0.8	0.4	0.1	0.2	0.7	0.7	0.5	0.1
E20C79	0.3	0.3	0.4	0.8	0.7	0.1	0.4	0.4	0.1	0.7	0.2
E20C82	0.1	0.3	0.3	0.2	0.3	0.3	0.2	0.7	0.2	0.3	0.5
E20C83	0.1	0.1	0.3	0.3	0.3	0.1	0.2	0.3	0.6	0.3	0.1
E21C9	0.2	0.3	0.3	0.2	0.1	0.1	0.2	0.1	0.2	0.1	0.1
E21C10	0.2	0.2	0.1	0.3	0.1	0.1	0.1	0.3	0.6	0.4	0.2
E21C11	1.4	2.2	2.1	0.4	0.2	0.4	0.1	0.2	0.1	0.3	0.3
E21C12	2.2	1.7	1.1	0.5	0.3	0.1	0.2	0.1	0.2	0.6	0.0
E21C13	1.4	1.6	1.5	0.3	0.2	0.4	0.1	0.2	0.1	0.3	0.2
E21C14	1.7	2.2	1.5	0.3	0.8	0.2	0.1	0.2	0.1	0.2	0.2
E21C56	0.5	0.3	0.4	0.4	0.4	0.1	0.2	0.5	0.1	0.3	0.1
E21C57	0.4	0.5	0.7	0.4	0.4	0.2	0.2	0.5	0.4	0.7	0.5
E21C58	0.6	0.8	0.6	0.7	0.8	0.5	0.5	0.4	0.2	0.5	0.4
E21C60	0.2	0.3	0.7	0.4	0.4	0.1	0.4	0.8	0.2	0.4	0.8
E21C62	1.2	0.6	0.7	0.4	0.4	0.4	0.7	0.4	0.7	0.5	0.3
E21C63	1.9	0.9	0.4	0.3	0.6	0.2	0.2	0.2	0.2	0.8	0.2
E21C64	0.9	0.9	0.5	0.2	0.8	0.7	0.2	0.4	0.4	0.5	0.5
E21C66	1.2	0.7	0.4	0.8	0.5	0.2	0.2	0.5	0.7	0.3	0.2
E21C67	1.5	0.8	0.7	0.4	0.8	0.2	0.7	0.8	0.4	0.2	0.2
E21C71	1.2	0.5	0.2	0.5	0.3	0.9	0.2	0.4	0.6	0.5	0.4
E21C72	0.3	0.4	0.3	0.6	0.7	0.2	0.2	0.2	0.2	0.4	0.9
E21C74	0.5	0.8	0.2	0.4	0.4	0.1	0.2	0.9	0.7	0.5	0.2
E21C79	0.5	0.6	0.8	0.4	0.4	0.1	0.2	0.2	0.1	0.5	0.2
E21C82	0.2	0.5	0.5	0.2	0.3	0.3	0.1	0.3	0.1	0.1	0.2
E21C83	0.2	0.2	0.5	0.3	0.3	0.1	0.1	0.1	0.3	0.1	0.0
E24C9	0.2	0.3	0.3	0.2	0.1	0.1	0.2	0.1	0.2	0.1	0.1
E24C10	0.2	0.2	0.1	0.3	0.1	0.4	0.2	0.7	0.6	0.4	0.2
E24C11	0.5	0.8	0.8	0.2	0.1	0.4	0.1	0.5	0.1	0.3	0.3
E24C12	0.8	0.6	0.4	0.2	0.1	0.1	0.2	0.4	0.2	0.6	0.0
E24C13	0.6	0.6	0.6	0.3	0.2	0.9	0.1	0.5	0.1	0.3	0.2
E24C14	0.6	0.9	0.6	0.3	0.4	0.3	0.1	0.2	0.0	0.1	0.1
E24C56	0.2	0.1	0.2	0.4	0.2	0.2	0.1	0.6	0.1	0.1	0.0
E24C57	0.2	0.2	0.3	0.4	0.2	0.1	0.0	0.2	0.2	0.6	0.3
E24C58	0.6	0.3	0.2	0.7	0.4	0.2	0.1	0.2	0.1	0.3	0.6
E24C60	0.2	0.1	0.1	0.2	0.2	0.2	0.2	0.4	0.1	0.3	0.4
E24C62	0.2	0.5	0.3	0.5	0.2	0.5	0.6	0.2	0.4	0.3	0.8
E24C63	0.2	0.7	0.2	0.4	0.4	0.2	0.1	0.1	0.1	0.4	0.1
E24C64	0.3	0.4	0.2	0.5	0.2	0.4	0.1	0.2	0.6	0.3	0.3
E24C66	0.4	0.5	0.1	0.3	0.2	0.1	0.1	0.2	0.4	0.3	0.1
E24C67	0.2	0.6	0.7	0.1	0.7	0.1	0.9	0.6	0.2	0.1	0.6
E24C71	0.3	0.5	0.1	0.1	0.2	0.5	0.3	0.2	0.3	0.3	0.2
E24C72	0.3	0.2	0.1	0.1	0.7	0.1	0.3	0.1	0.1	0.3	0.7
E24C74	0.5	0.3	0.1	0.2	0.5	0.2	0.3	0.5	0.4	0.3	0.1
E24C79	0.5	0.2	0.3	0.2	0.9	0.2	0.5	0.2	0.1	0.5	0.2
E24C82	0.4	0.5	0.5	0.2	0.9	0.8	0.2	0.3	0.1	0.1	0.2
E24C83	0.4	0.2	0.5	0.3	0.7	0.2	0.2	0.1	0.3	0.1	0.0
Lip3000	1.0

Embodiment 13: Transfection of Lipid Nanoparticles Prepared from Amino Compounds on BMDC Primary Cells

• • Preparation method: the same as that described in Embodiment 7.
  • Animal preparation: 6-week-old female C57BL/6 mice with the body weight about 20 g were selected. The feeding environment was an SPF stage feeding room. Animal tests were strictly performed according to the guide of the national health institute and the animal ethics requirements.
  • Cell acquisition: C57BL/6 mice were killed through cervical dislocation, and were soaked for 5 min in 75% ethyl alcohol. Dissection was performed to obtain thigh and calf tibiae of the mice. Attached muscles were removed to expose sclerotin. Then, bone marrow in tibiae was blown out by using a 1 mL injector sucked with PBS. After the bone marrow was blown away, impurities were filtered away by a 50 μm filter screen. A red blood cell lysis buffer (3-4 mL) was added into an obtained filtrate. Then, after placement for 5 min, 800 g centrifugation was performed for 5 min to remove the supernatant. The obtained cells were placed into a 1640 culture medium (containing 10% fetal calf serum, 20 ng/mL GMCSF and 10 ng/mL IL4) to be resuspended, and were inoculated into a 6-well plate at an inoculation density of 100000 cells/ml culture medium. The materials were placed into a 37° C. cell incubator containing 5% CO₂. Half liquid change was performed once every 2 days. Suspended cells and loose wall attached cells were collected on the seventh day, and were inoculated to a 96-well all-white ELISA plate at the inoculation density of 20000 cells per well, and the volume of a culture medium was 100 μL.
  • Cell transfection: lipid nanoparticles coated with luciferase mRNA were added into a 96-well all-white ELISA plate laid with primary cells. The adding volume of the mRNA lipid nanoparticles in each well was controlled to be 10 μL. Then, the materials were put into a 37° C. incubator containing 5% CO₂ for 16 h.
  • Transfection efficiency detection: 20 μL of a substrate ONE-Glo™ Luciferase was added into each well of a 96-well all-white ELISA plate. After 1 min, detection was performed by a multifunctional microplate reader (Biorek SynergyH1). The expression intensity of the LucmRNA transfection of representative amino lipid compounds on BMDC was as shown in Table 3. DLin-MC3 was used as a control, a plurality of amino lipids had similar expression intensity to MC3, and a plurality of amino lipids had the expression intensity obviously superior to that of the positive control.

TABLE 3
Transfection expression intensity of 98 amino lipid compounds on
BMDC
	Serial		Fluo-
Se-	number		res-
rial	of		cence
num-	amino		inten-
ber	lipid	Structure	sity
1	E3C 79A10		7.4E+ 04
2	E3C 79A11		9.0E+ 04
3	E3C 79A12		6.7E+ 04
4	E4C 72A11		5.8E+ 04
5	E4C 74A9		6.9E+ 04
6	E4C 74A10		4.7E+ 04
7	E4C 74A11		6.0E+ 04
8	E4C 79A9		2.2E+ 04
9	E4C 79A10		1.3E+ 04
10	E4C 79A11		3.4E+ 04
11	E4C 79A12		2.5E+ 04
12	E5C 13A12		1.1E+ 04
13	E5C 71A11		4.6E+ 04
14	E5C 74A10		2.9E+ 04
15	E5C 74A11		7.7E+ 03
16	E5C 79A11		3.6E+ 04
17	E5C 82A23		1.4E+ 04
18	E6C 71A11		3.4E+ 04
19	E6C 71A12		7.3E+ 04
20	E6C 72A11		5.0E+ 04
21	E6C 74A10		1.1E+ 04
22	E6C 74A11		4.5E+ 04
23	E6C 79A10		4.6E+ 04
24	E6C 79A11		5.4E+ 04
25	E6C 79A12		2.5E+ 04
26	E7C 71A9		8.8E+ 04
27	E7C 71A10		8.3E+ 04
28	E7C 71A11		4.4E+ 04
29	E7C 74A9		4.8E+ 04
30	E7C 74A10		3.7E+ 04
31	E7C 74A11		3.2E+ 04
32	E7C 79A9		5.6E+ 04
33	E7C 79A10		3.6E+ 04
34	E7C 79A11		4.2E+ 04
35	E7C 79A12		3.5E+ 04
36	E8C 71A9		8.8E+ 04
37	E8C 71A11		5.4E+ 04
38	E8C 71A23		1.5E+ 04
39	E8C 72A9		6.1E+ 04
40	E8C 72A11		7.3E+ 04
41	E8C 74A9		8.5E+ 04
42	E8C 74A11		4.0E+ 04
43	E8C 74A23		1.3E+ 04
44	E8C 79A9		3.6E+ 04
45	E9C 56A10		8.8E+ 04
46	E9C 57A10		3.2E+ 04
47	E9C 58A11		3.5E+ 04
48	E9C 60A23		1.8E+ 04
49	E9C 62A10		4.3E+ 04
50	E9C 63A10		5.6E+ 04
51	E9C 64A9		8.9E+ 04
52	E9C 64A10		6.1E+ 04
53	E9C 66A9		2.3E+ 04
54	E9C 67A10		7.8E+ 04
55	E10C 74A9		1.3E+ 04
56	E10C 74A23		1.0E+ 04
57	E10C 79A9		1.3E+ 04
58	E11C 7A9		1.5E+ 04
59	E11C 57A11		6.4E+ 04
60	E11C 58A11		2.3E+ 04
61	E11C 60A11		3.3E+ 04
62	E11C 62A11		4.1E+ 04
63	E12C 6A11		1.2E+ 04
64	E12C 64A23		1.8E+ 04
65	E12C 74A11		1.1E+ 04
66	E15C 79A10		8.9E+ 04
67	E15C 79A11		3.0E+ 04
68	E15C 79A12		4.4E+ 04
69	E20C 9A10		2.7E+ 04
70	E20C 9A11		4.0E+ 04
71	E20C 10A9		4.8E+ 04
72	E20C 10A10		1.2E+ 04
73	E20C 10A11		1.9E+ 04
74	E20C 10A12		3.6E+ 04
75	E20C 11A9		9.9E+ 04
76	E20C 11A10		4.5E+ 04
77	E20C 11A23		1.6E+ 04
78	E20C 12A9		4.9E+ 04
79	E20C 12A10		9.6E+ 04
80	E20C 12A23		1.7E+ 04
81	E20C 64A9		3.0E+ 04
82	E20C 66A9		5.9E+ 04
83	E20C 67A9		9.5E+ 03
84	E20C 71A9		6.6E+ 04
85	E20C 72A9		4.7E+ 03
86	E21C 11A9		8.7E+ 03
87	E21C 11A10		1.7E+ 04
88	E21C 11A11		4.7E+ 04
89	E21C 12A9		5.4E+ 04
90	E21C 12A10		8.9E+ 04
91	E21C 12A11		9.5E+ 04
92	E21C 13A9		6.7E+ 04
93	E21C 13A11		6.4E+ 04
94	E21C 14A9		4.5E+ 04
95	E21C 67A9		3.2E+ 04
96	E21C 71A23		1.9E+ 04
97	E24C 9A9		1.1E+ 04
98	DLin-		2.1E+
	MC3		04

Embodiment 14: Evaluation on Luciferase mRNA In-Vivo Delivery Performance of Lipid Nanoparticles Prepared from Amino Lipid Compounds

1. Preparation of Lipid Nanoparticles

The amino lipid compounds of the disclosure, neutral lipids (such as DSPC, DOPE and cholesterin) and polyethylene glycolated lipids (such as PEG2000-DMG and PEG2000-DSPE) were mixed according to an optimized mole ratio and were dissolved in absolute ethyl alcohol. The obtained ethyl alcohol solution and a sodium acetate buffer solution (25 mM, pH=5.0) dissolved with Luc-mRNA (TriLink) were mixed according to a volume ratio of 1:3 by using a micro-fluidic preparation system to prepare a coarse solution of the lipid nanoparticles. Then, the coarse solution was dialyzed for 6 h under the condition of 1×PBS and temperature control at 4° C. by a dialysis box (Fisher, MWCO 20,000). Filtration was performed by a 0.22 μm microporous filtering membrane prior to use. A mass ratio of the amino lipid compound to luciferase mRNA (Luc mRNA) was about 10:1.

Characterization of Lipid Nanoparticles

Characterization of particle size: the particle size and PDI of the prepared lipid nanoparticles were measured through Nano-ZSZEN3600 (Malvern). 20 μL of the LNP solution was taken for particle size measurement. Three times were performed, and each time lasted for 30 s.

Encapsulation efficiency determination: the determination was performed with the reference to a Quant-iT RiboGreen RNA kit standard procedure.

TABLE 4
Characterization data of LNP prepared from
representative amino lipid compounds
Serial	Serial number of	Z-Average		Encapsulation
number	amino lipid	(d · nm)	PDI	efficiency
LNP-1	E4C79A9	122.4	0.12	93.9%
LNP-2	E4C79A11	124.6	0.14	94.1%
LNP-3	E4C79A12	122.1	0.07	95.3%
LNP-4	E5C71A11	123.1	0.10	93.5%
LNP-5	E5C79A11	131.2	0.07	95.6%
LNP-6	E6C71A11	124.1	0.13	93.0%
LNP-7	E6C71A12	110.1	0.09	93.6%
LNP-8	E6C79A10	129.2	0.05	94.4%
LNP-9	E6C79A11	141.2	0.08	95.6%
LNP-10	E6C79A12	123.4	0.10	93.7%
LNP-11	E7C71A9	112.1	0.02	96.6%
LNP-12	E7C71A10	126.8	0.09	95.3%
LNP-13	E7C79A9	129.8	0.08	94.9%
LNP-14	E7C79A10	148.3	0.04	95.2%
LNP-15	E7C79A11	140.1	0.02	95.4%
LNP-16	E8C71A9	147.6	0.09	93.1%
LNP-17	E8C71A11	136.4	0.04	94.8%
LNP-18	E8C71A12	139.5	0.02	95.0%
LNP-19	E8C79A9	123.6	0.06	91.8%
LNP-20	E9C56A10	140.9	0.15	95.9%
LNP-21	E9C57A10	140.4	0.13	92.2%
LNP-22	E9C62A10	139.2	0.02	93.0%
LNP-23	E9C64A9	150.4	0.12	94.4%
LNP-24	E9C64A10	143.7	0.12	95.2%
LNP-25	E9C71A10	139.9	0.12	92.5%
LNP-26	E11C57A11	157.6	0.08	93.6%
LNP-27	E11C60A11	103.7	0.05	92.9%
LNP-28	E11C62A11	102.6	0.05	91.4%
LNP-29	E15C79A11	100.2	0.02	93.3%
LNP-30	E15C79A12	107.1	0.08	92.1%
LNP-31	E20C10A12	111.9	0.11	93.4%
LNP-32	E20C11A10	109.8	0.06	94.0%
LNP-33	E20C12A9	103.3	0.09	93.6%
LNP-34	E20C71A9	107.2	0.14	95.3%
LNP-35	E21C11A11	101.1	0.12	94.1%
LNP-36	E21C12A9	109.7	0.05	95.0%
LNP-37	E6C71A12	139.3	0.10	93.5%
LNP-38	E7C71A9	141.7	0.09	92.6%
LNP-39	DLin-MC3	139.2	0.15	94.6%
LNP-40	E6C71A12	139.8	0.05	93.5%
LNP-41	E7C71A9	141.3	0.10	94.6%
LNP-42	E6C71A12	137.3	0.08	93.6%
LNP-43	E7C71A9	140.1	0.09	93.5%
LNP-44	E6C71A12	134.7	0.13	94.6%
LNP-45	E7C71A9	138.9	0.11	94.5%
LNP-46	E6C71A12	132.3	0.04	93.4%
LNP-47	E7C71A9	124.2	0.06	94.5%

Note: in the above table:

• • the lipid formulation of LNP-1 to LNP-36 was as follows: amino lipid:DSPC:cholesterol:PEG2000-DMG=50:10:38.5:1.5;
  • the lipid formulation of LNP-37 to LNP-39 was as follows: amino lipid:DOPE:cholesterol:PEG2000-DMG=45:10:42.5:1.5;
  • the lipid formulation of LNP-40 to LNP-41 was as follows: amino lipid:DOPC:cholesterol:PEG2000-DMG=55:5:38.5:1.5;
  • the lipid formulation of LNP-42 to LNP-43 was as follows: amino lipid:cholesterol:PEG2000-DSPE=60:35.5:4.5;
  • the lipid formulation of LNP-44 to LNP-45 was as follows: amino lipid:DSPC:DOPC:cholesterol:PEG2000-DMG=45:10:5:38.0:2.0; and
  • the lipid formulation of LNP-46 to LNP-47 was as follows: amino lipid:DSPC:DOPE:cholesterol:PEG2000-DSPE=50:10:5:33.5:1.5.

2. Animal Tests

Animal preparation: 6-week-old female C₅₇BL/6 mice with the body weight about 20 g were selected. The feeding environment was an SPF stage feeding room. Animal tests were strictly performed according to the guide of the national health institute and the animal ethics requirements.

In-vivo delivery: 9 C57BL/6 mice were randomly selected for each group. According to the mRNA standard of 0.5 mg/kg, the lipid nanoparticle solution was respectively injected in three administration manners of subcutaneous, intramuscular and tail intravenous injection (3 mice for each administration manner). After 12 h, 200 μL of 10 mg/mL D-luciferin potassium salt was injected into each mouse through tail intravenous injection. After 10 min, the mice were placed in an in-vivo living imaging system (IVIS-200, Xenogen), the total fluorescence intensity of each mouse was observed, and photos were taken for recording. The expression intensity of the Fluc mRNA delivered by the representative amino lipid compounds in 3 administration manners was as shown in Table 5 to Table 7. DLin-MC3 was used as a control.

TABLE 5
Expression intensity of Luc mRNA delivered by LNP subcutaneous
administration of representative amino lipid compounds
Serial	Serial number	Average fluorescence
number	of LNP	intensity
1	LNP-1	2.4E+06
2	LNP-4	5.3E+06
3	LNP-5	1.5E+06
4	LNP-6	7.9E+06
5	LNP-7	7.0E+07
6	LNP-11	8.9E+07
7	LNP-16	6.6E+06
8	LNP-17	3.1E+06
9	LNP-20	7.7E+06
10	LNP-25	2.5E+06
11	LNP-29	6.3E+07
12	LNP-32	1.8E+06
13	LNP-34	5.9E+06
14	LNP-35	4.8E+06
15	LNP-37	5.8E+06
16	LNP-38	1.4E+06
17	LNP-39	4.5E+06
18	LNP-43	5.7E+07

TABLE 6
Expression intensity of Luc mRNA delivered by LNP intramuscular
injection administration of representative amino lipid compounds
Serial	Serial number	Fluorescence
number	of LNP	intensity
1	LNP-2	4.3E+06
2	LNP-7	5.1E+07
3	LNP-8	4.5E+06
4	LNP-11	7.6E+07
5	LNP-12	1.9E+06
6	LNP-14	8.6E+06
7	LNP-15	3.1E+06
8	LNP-23	9.7E+06
9	LNP-26	3.3E+06
10	LNP-27	7.7E+06
11	LNP-30	2.8E+06
12	LNP-39	7.4E+06
13	LNP-40	3.5E+06
14	LNP-41	2.7E+06
15	LNP-42	6.5E+06
16	LNP-45	3.4E+07
17	LNP-46	8.5E+06
18	LNP-47	5.7E+06

TABLE 7
Expression intensity of Luc mRNA delivered by LNP tail intravenous
administration of representative amino lipid compounds
Serial	Serial number	Fluorescence
number	of LNP	intensity
1	LNP-3	3.4E+06
2	LNP-7	8.3E+07
3	LNP-9	5.9E+06
4	LNP-10	1.9E+07
5	LNP-11	7.0E+07
6	LNP-13	4.9E+06
7	LNP-18	5.6E+06
8	LNP-19	3.9E+07
9	LNP-21	1.1E+07
10	LNP-22	5.7E+06
11	LNP-24	6.4E+06
12	LNP-28	7.9E+06
13	LNP-31	5.8E+06
14	LNP-33	2.6E+06
15	LNP-39	4.7E+06
16	LNP-42	8.4E+07
17	LNP-43	7.5E+07
18	LNP-44	5.3E+06

Embodiment 15: In-Vivo Immunity and Tumor Treatment Effect Evaluation of Lipid Nanoparticles Prepared from Amino Lipid Compounds

• • Preparation method: the amino lipid compounds of the disclosure, DSPC, cholesterol and PEG2000-DMG were mixed according to a mole ratio of 50:10:38.5:1.5 and were dissolved in absolute ethyl alcohol. OVA mRNA was dissolved in a sodium acetate buffer solution (50 nM, pH=4.0). The obtained ethyl alcohol solution and the acetate buffer solution (25 nM, pH=5.0) dissolved with Luc-mRNA (TriLink) were mixed in a micro-fluidic chip according to a volume ratio of 1:3 by using a micro-fluidic preparation system to obtain lipid nanoparticles. Then, dialysis was performed for 6 h under the conditions of 1×PBS and temperature was controlled at 4° C. by using a dialysis box (Fisher, MWCO 20,000). Filtration was performed by a 0.22 μm microporous filtering membrane prior to use. The mass ratio of the amino lipid compound to OVA mRNA was about 10:1.
  • Animal preparation: 5-6-week-old female C₅₇BL/6 mice with the body weight about 18 to 20 g were selected. The feeding environment was an SPF stage feeding room. Animal tests were strictly performed according to the guide of the national health institute and the animal ethics requirements.
  • In-vivo delivery: B16-OVA melanoma cells (1.5×10⁵) were injected to the outer thighs of the mice through subcutaneous injection. When the tumor grew to 50 mm³ (on the about 6^th or 7^th day after tumor inoculation), the mice began to inoculated vaccines, the animals were subjected to twice immunization through intramuscular injection of an LNP preparation containing 1 μg OVA-mRNA. An interval between first and second injections was 7 days. The tumor growth was measured for 3 times every week by a digital display calliper, and a calculation formula was 0.5×length×width. When the tumor size reached 1500 mm³, the mice received euthanasia. The tumor growth speeds of E7C71A9 and E6C71A12 were obviously lower than that of MC3 group (as shown in FIG. 3). Additionally, 60% of mice (E7C71A9 group) and 40% of mice (E6C71A12 group) respectively reached the complete relieving effect. The result was obviously superior to that of MC3 group (as shown in FIG. 4).

Embodiment 16: 2-hydroxydodecyl-2-heptylnonanoate

FeCl₃ (20 mg, 0.125 mmol), Py (5 μL, 0.0625 mmol), 2-heptyl pelargonic acid (1.28 g, 5 mmol) and 1,2-cyclododecane epoxide (1.84 g, 10 mmol) were sequentially added into a 25 mL reaction tube, and the reaction was stirred at room temperature overnight. The column chromatography gradient elution purification (hexane:EA=20:1 to 5:1) was performed to obtain 2-hydroxydodecyl-2-heptylnonanoate (1.54 g, 70% yield). ¹H NMR (400 MHz, CDCl₃): δ 0.85-0.89 (m, 9H), 1.25-1.26 (m, 36H), 1.39-1.41 (m, 2H), 1.58-1.62 (m, 4H), 2.11-2.14 (m, 1H), 4.05-4.11 (m, 2H), 4.33-4.35 (m, 1H), 5.37 (brs, 1H). ESI-MS calculated for C₂₈H₅₇O₃⁺ [M+H]⁺ 441.4, found 441.7.

Embodiment 17: 2-((4-(dimethylamino)butanoyl)oxy)dodecyl 2-heptylnonanoate

EDC·HCl (192 mg, 1 mmol), DIPEA (174 μL, 1 mmol), DMAP (3.0 mg, 0.025 mmol), 4-(dimethylamino)butanoic acid (101 mg, 0.6 mmol), 2-hydroxy dodecyl 2-heptyl pelargonate (220 mg, 0.5 mmol) and DCM (4 mL) were sequentially added into a 10 mL reaction tube, and the reaction was stirred for 3 h at room temperature. The column chromatography gradient elution purification (DCM:MeOH=100:1 to 100:3) was performed to obtain the compound E7C114A9 (222 mg, 80% yield). ¹H NMR (400 MHz, CDCl₃): δ 0.85-0.89 (m, 9H), 1.25-1.44 (m, 38H), 1.55-1.58 (m, 4H), 1.75-1.83 (m, 2H), 2.23 (s, 6H), 2.28-2.39 (m, 5H), 4.01-4.06 (dd, J₁=11.7 Hz, J₂=6.1 Hz, 1H), 4.21-4.25 (dd, J₁=11.8 Hz, J₂=3.5 Hz, 1H), 5.05-5.07 (m, 1H). ¹³C NMR (100 MHz, CDCl₃): δ 14.1, 22.7, 23.4, 25.3, 29.3, 29.4, 29.6, 31.9, 32.7, 44.7, 47.0, 61.0, 65.8, 70.7, 173.1, 175.8. ESI-MS calculated for C₃₄H₆₈NO₄⁺[M+H]⁺ 554.5, found 554.4.

Embodiment 18: 2-hydroxytetradecyl-3-hexylundecanoate

FeCl₃ (20 mg, 0.125 mmol), Py (5 μL, 0.0625 mmol), 3-hexyl undecanoic acid (1.36 g, 5 mmol) and 1,2-epoxytetradecane (2.12 g, 10 mmol) were sequentially added into a 25 mL reaction tube, and the reaction was stirred at room temperature overnight. The column chromatography gradient elution purification (hexane:EA=20:1 to 5:1) was performed to obtain 2-hydroxytetradecyl 3-hexylundecanoate (1.59 g, 66% yield). ¹H NMR (400 MHz, CDCl₃): δ 0.87-0.90 (m, 9H), 1.24-1.28 (m, 44H), 1.39-1.41 (m, 2H), 1.92-1.95 (m, 1H), 2.02-2.04 (m, 1H), 2.26-2.29 (m, 1H), 4.09-4.14 (m, 2H), 4.33-4.35 (m, 1H), 5.38 (brs, 1H). ESI-MS calculated for C₃₁H₆₃O₃⁺ [M+H]⁺ 483.5, found 483.8.

Embodiment 19: 2-((3-(piperidin-1-yl)propanoyl)oxy)tetradecyl-3-hexylundecanoate

EDC·HCl (192 mg, 1 mmol), DIPEA (174 μL, 1 mmol), DMAP (3.0 mg, 0.025 mmol), 3-piperidine-1-propionic acid (95 mg, 0.6 mmol), 2-hydroxytetradecyl 3-hexylundecanoate (242 mg, 0.5 mmol) and DCM (4 mL) were sequentially added into a 10 mL reaction tube, stirring reaction was performed for 3 h at a room temperature, and column chromatography gradient elution purification (DCM:MeOH=100:1 to 100:3) was performed to obtain a compound E9C126A24 (227 mg, 73% yield). ¹H NMR (400 MHz, CDCl₃): δ 0.87-0.80 (m, 9H), 1.23-1.28 (m, 44H), 1.38-1.39 (m, 2H), 1.44-1.49 (m, 6H), 1.92-1.95 (m, 1H), 2.02-2.05 (m, 1H), 2.27-2.29 (m, 1H), 2.35-2.43 (m, 6H), 3.76-3.79 (m, 2H), 4.17-4.19 (m, 1H), 4.42-4.44 (m, 1H), 5.16-5.18 (m, 1H). ¹³C NMR (100 MHz, CDCl₃): δ 14.1, 22.7, 24.5, 25.3, 25.9, 27.1, 29.3, 29.6, 29.9, 30.7, 31.8, 31.9, 32.4, 32.6, 33.2, 39.7, 52.8, 56.8, 65.5, 70.7, 173.1. ESI-MS calculated for C₃₉H₇₆NO₄⁺ [M+H]⁺ 622.6, found 622.9.

Embodiment 20: In-Vitro Evaluation of Amino Lipid Compound as mRNA Vector

• • Cell line: HeLa cell line (ATCC)
  • Culture medium: 1640 (Invitrogen) supplemented with 10% fetal calf serum
  • Screening form: 96-well plate cell transfection
  • Detection: fluorescence intensity detection by a multifunctional microplate reader. According to manufacturer's Instructions, Lipofectamine3000 (Invitrogen) was used as a positive control group.
  • Method: an 8-channel pipette was used for sample addition. The shown content is the content of a single well of a 96-well plate.
  • 1. Synthesis was performed with reference to a route in Embodiment 17 and Embodiment 19 to obtain a serious of amino lipid compounds. The amino lipid compounds were mixed with DSPC, cholesterol and PEG2000-DMG according to a mole ratio of 47.5:10:41:1.5 in absolute ethyl alcohol. The Luc-mRNA (TriLink) was dissolved into a sodium acetate buffer solution (25 nM, pH=5.0). The mixed lipid solution was taken out by a multi-channel pipette tip, and was added into the Luc-mRNA solution to be sufficiently mixed. A proportion ratio of the ethyl alcohol solution to the sodium acetate buffer solution (25 nM, pH=5.0) was controlled to be 1:3, and a nanoparticle solution was prepared. The mass ratio of the amino lipid compound to luciferase mRNA (Luc mRNA) was about 10:1, and the mRNA consumption in each well was 100 ng.
  • 2. After the lipid nanoparticle solution was incubated for 30 min at a room temperature, 100 μL of fresh resuspended HeLa cells (1×10⁴ cells) were added into each well of a 96-well all-white ELISA plate. Then, the lipid nanoparticle solution was added into the 96-well plate (10 μL for each well) by a pipette. The solution was placed into a 37° C. incubator containing 5% CO₂ to be incubated.
  • 3. After 16 h to 20 h of cell initial transfection, a substrate ONE-Glo™ Luciferase was added into cells at 100 μL/well, and after 2 min, detection was performed by a multifunctional microplate reader (Biorek SynergyH1).
  • 4. The relative transfection efficiency was calculated as follows:

Relative transfection efficiency (%)=fluorescence intensity of LNP/fluorescence intensity of Lip3000×100%.

Result: the transfection efficiency of parts of compounds on Luc-mRNA of the HeLa cells is shown in Table 8.

TABLE 8
relative transfection efficiency of 462 kinds
of compounds on mRNA of the HeLa cells
	A9	A11	A12	A16	A23	A24
E3C116	1.2	3.2	0.9	0.7	0.7	0.8
E5C104	1.5	1.3	0.7	0.8	0.9	0.9
E5C106	0.8	0.9	0.6	0.5	0.4	0.6
E5C109	1.1	0.5	0.4	0.3	0.5	0.4
E5C111	0.8	1.2	0.7	0.9	0.5	0.3
E5C114	1.4	2.1	0.5	0.4	0.4	0.6
E5C115	1.2	6.1	0.9	0.7	0.5	0.5
E5C116	1.2	3.0	0.8	0.6	0.6	0.5
E5C119	1.1	0.8	0.6	0.4	0.9	0.2
E5C123	0.7	0.8	0.6	0.3	0.2	0.5
E5C126	0.4	0.5	0.1	0.1	0.4	0.2
E5C128	1.2	1.5	0.8	0.9	0.8	0.4
E5C131	0.3	0.1	0.4	0.2	0.4	0.5
E5C135	0.9	0.4	0.41	0.3	0.6	0.6
E7C104	1.3	0.9	0.7	0.5	0.6	0.1
E7C106	1.1	0.3	0.6	0.5	0.6	0.3
E7C109	0.5	1.1	0.5	0.8	0.7	0.2
E7C111	0.1	0.2	0.9	0.7	0.7	0.2
E7C114	4.6	5.9	0.9	0.7	0.5	0.4
E7C115	1.6	8.3	1.0	0.9	0.3	0.4
E7C116	1.1	5.0	0.6	0.8	0.3	0.5
E7C119	0.7	0.8	1.0	0.7	0.9	0.2
E7C123	1.3	0.9	0.7	0.4	0.3	0.2
E7C126	0.6	1.1	0.4	0.2	0.5	0.1
E7C128	1.00	1.3	0.8	0.7	0.4	0.6
E7C131	0.2	0.4	0.2	0.1	0.2	0.2
E7C135	0.8	0.8	0.7	0.5	0.4	0.2
E8C104	1.6	0.9	0.2	0.3	0.5	0.3
E8C106	0.9	0.4	0.5	0.3	0.6	0.5
E8C109	1.6	0.3	0.6	0.8	0.6	0.7
E8C111	1.3	0.7	0.3	0.2	0.4	0.9
E8C114	2.9	4.8	0.4	0.3	0.5	0.3
E8C115	1.7	6.2	0.9	0.9	0.7	0.6
E8C116	1.2	3.1	0.7	0.8	0.2	0.5
E8C119	0.9	0.5	0.5	0.3	0.7	0.2
E8C123	0.8	2.0	0.6	0.4	0.3	0.4
E8C126	0.3	0.9	0.8	0.7	0.6	0.6
E8C128	2.1	1.0	0.9	0.6	0.4	0.4
E8C131	0.8	0.8	0.5	0.2	0.3	0.3
E8C135	0.9	0.5	0.6	0.5	0.5	0.2
E9C104	1.5	1.8	0.6	0.6	0.8	0.4
E9C106	0.7	0.5	0.5	0.5	0.6	0.5
E9C109	1.8	1.6	0.8	0.5	0.6	0.5
E9C111	0.9	1.3	0.6	0.7	0.4	0.3
E9C114	3.6	4.9	0.9	0.9	0.8	0.6
E9C115	6.0	7.5	1.0	0.8	0.7	0.8
E9C116	1.1	4.7	0.4	1.0	0.3	0.3
E9C119	0.9	0.9	0.8	0.5	0.4	0.6
E9C123	0.4	2.4	0.6	0.3	0.5	0.3
E9C126	0.6	0.4	0.3	0.4	0.3	0.3
E9C128	1.8	1.6	0.8	0.5	0.4	0.2
E9C131	0.6	0.5	0.4	0.6	0.5	0.4
E9C135	0.7	0.7	0.8	0.6	0.3	0.2
E10C104	0.5	0.4	0.6	0.5	0.3	0.5
E10C106	1.6	0.6	0.5	0.5	0.4	0.4
E10C109	0.7	1.1	0.9	0.7	0.7	0.8
E10C111	1.1	0.5	0.7	0.4	0.7	0.5
E10C114	1.2	2.5	0.9	0.8	0.7	0.2
E10C115	1.2	2.7	0.7	0.6	0.6	0.8
E10C119	0.3	0.9	0.8	0.5	0.3	0.4
E10C123	0.6	0.7	0.4	0.7	0.4	0.2
E10C126	0.4	0.8	0.6	0.6	0.9	0.5
E10C128	1.1	1.4	0.2	0.3	0.2	0.4
E10C131	0.5	0.5	0.6	0.5	0.8	0.1
E10C135	0.9	0.6	0.7	0.5	0.7	0.6
E11C104	0.7	0.9	0.6	0.8	0.5	0.5
E11C106	0.8	0.7	0.8	0.5	0.4	0.3
E11C109	1.3	1.4	0.3	0.8	0.5	0.4
E11C111	0.5	0.6	0.5	0.6	0.8	0.6
E11C114	1.6	1.9	0.9	0.3	0.6	0.5
E11C115	1.3	1.8	0.6	0.7	0.5	0.6
E11C119	0.8	0.8	0.7	0.5	0.5	0.4
E11C123	0.9	0.8	0.9	0.8	0.6	0.5
E11C126	1.5	0.9	0.8	0.5	0.6	0.3
E11C128	1.1	1.2	0.9	0.8	0.3	0.7
E11C131	0.2	0.7	0.4	0.5	0.3	0.3
E11C135	0.4	0.3	0.6	0.6	0.5	0.2

Embodiment 21: Transfection of Lipid Nanoparticles Prepared from Amino Compounds on BMDC Primary Cells

• • Preparation method: the same as that in Embodiment 17.
  • Animal preparation: 6-week-old female C₅₇BL/6 mice with the body weight about 20 g were selected. A feeding environment was an SPF stage feeding room. Animal tests were strictly performed according to the guide of the national health institute and the animal ethics requirements.
  • Cell acquisition: C57BL/6 mice were killed through cervical dislocation, and were soaked for 5 min in 75% ethyl alcohol. Dissection was performed to obtain thigh and calf tibiae of the mice. Attached muscles were removed to expose sclerotin. Then, bone marrow in tibiae was blown out by using a 1 mL injector sucked with PBS. After the bone marrow was blown away, impurities were filtered away by a 50 μm filter screen. A red blood cell lysis buffer (3-4 mL) was added into an obtained filtrate. Then, after placement for 5 min, 800 g centrifugation was performed for 5 min to remove a supernatant. The obtained cells were placed into a 1640 culture medium (containing 10% fetal calf serum, 20 ng/mL GMCSF and 10 ng/mL IL4) to be resuspended, and were inoculated into a 6-well plate at an inoculation density of 100000 cells/ml culture medium. The materials were placed into a 37° C. cell incubator containing 5% CO₂. Half liquid change was performed once every 2 days. Suspended cells and loose wall attached cells were collected on the seventh day, and were inoculated to a 96-well all-white ELISA plate at the inoculation density of 20000 cells per well, and the volume of a culture medium was 100 μL.
  • Cell transfection: lipid nanoparticles coated with luciferase mRNA were added into a 96-well white ELISA plate laid with primary cells. The adding volume of the mRNA lipid nanoparticles in each well was controlled to be 10 μL. Then, the materials were put into a 37° C. incubator containing 5% C₀₂ for 16 h.
  • Transfection efficiency detection: 20 μL of a substrate ONE-Glo™ Luciferase was added into each well of a 96-well all-white ELISA plate, and after 1 min, detection was performed by a multifunctional microplate reader (Biorek SynergyH1). The expression intensity of the LucmRNA transfection of representative amino lipid compounds on BMDC was as shown in Table 9. DLin-MC3 was used as a control, a plurality of amino lipids had similar expression intensities to MC3, and a plurality of amino lipids had the expression intensities obviously superior to those of the positive control.

TABLE 9
Expression intensity of transfection of 65 amino lipid compounds on
BMDC
Serial	Serial number of		Fluorescence
number	amino lipid	Structure	intensity
1	E3C116A11		4.4E+04
2	E5C104A9		5.2E+04
3	E5C109A9		3.9E+03
4	E5C114A9		8.6E+03
5	E5C115A9		2.7E+04
6	E5C119A9		4.1E+04
7	E5C128A9		3.9E+04
8	E5C104A11		4.0E+04
9	E5C114A11		4.6E+04
10	E5C115A11		1.1E+05
11	E5C116A11		6.2E+04
12	E5C128A11		4.3E+04
13	E7C104A9		3.9E+04
14	E7C106A9		3.9E+04
15	E7C114A9		6.6E+04
16	E7C115A9		5.6E+04
17	E7C123A9		4.3E+04
18	E7C128A9		3.5E+04
19	E7C114A11		9.9E+04
20	E7C115A11		2.4E+05
21	E7C116A11		9.2E+04
22	E7C126A11		3.9E+04
23	E7C128A11		3.6E+04
24	E8C104A9		3.7E+04
25	E8C109A9		4.8E+04
26	E8C111A9		4.6E+04
27	E8C114A9		6.8E+04
28	E8C115A9		6.0E+04
29	E8C128A9		3.9E+04
30	E8C114A11		1.3E+05
31	E8C115A11		1.9E+05
32	E8C116A11		5.0E+04
33	E8C123A11		7.1E+04
34	E8C128A11		3.5E+04
35	E9C104A9		3.6E+04
36	E9C109A9		3.6E+04
37	E9C114A9		9.1E+04
38	E9C115A9		1.4E+05
39	E9C128A9		3.7E+04
40	E9C104A11		4.4E+04
41	E9C109A11		3.6E+04
42	E9C111A11		3.9E+04
43	E9C114A11		8.5E+04
44	E9C115A11		1.9E+05
45	E9C116A11		7.6E+04
46	E9C123A11		5.1E+04
47	E9C128A11		3.5E+04
48	E10C106A9		4.2E+04
49	E10C111A9		3.7E+04
50	E10C114A9		4.3E+04
51	E10C115A9		4.4E+04
52	E10C128A9		3.8E+04
53	E10C109A11		3.5E+04
54	E10C114A11		7.6E+04
55	E10C115A11		8.2E+04
56	E10C128A11		4.7E+04
57	E11C109A9		4.3E+04
58	E11C114A9		4.5E+04
59	E11C115A9		4.9E+04
60	E11C126A9		3.6E+04
61	E11C128A9		3.9E+04
62	E11C109A11		4.8E+04
63	E11C114A11		6.5E+04
64	E11C115A11		6.3E+04
65	E11C128A11		4.2E+04

Embodiment 22: Evaluation of Luciferase mRNA In-Vivo Delivery Performance of Lipid Nanoparticles Prepared from Amino Lipid Compound

1. Preparation of Lipid Nanoparticles

The amino lipid compounds of the disclosure, neutral lipids (such as DSPC, DOPE and cholesterin) and polyethylene glycolated lipids (such as PEG2000-DMG and PEG2000-DSPE) were mixed in absolute ethyl alcohol according to an optimized mole ratio. The obtained ethyl alcohol solution and a sodium acetate buffer solution (25 mM, pH=5.0) dissolved with Luc-mRNA (TriLink) were mixed according to a volume ratio of 1:3 by using a micro-fluidic preparation system to prepare a coarse solution of the lipid nanoparticles. Then, the coarse solution was dialyzed for 6 h under the condition of 1×PBS and temperature control at 4° C. by using a dialysis box (Fisher, MWCO 20,000). Before use, filtration was performed by a 0.22 μm microporous filtering membrane. A mass ratio of the amino lipid compound to luciferase mRNA (Luc mRNA) was about 10:1.

Expression of Lipid Nanoparticles

Expression of particle size: the particle size and PDI of the prepared lipid nanoparticles were measured through Nano-ZSZEN3600 (Malvern). 20 μL of the LNP solution was taken for particle size measurement. Three times were performed and each time lasted for 30 s.

Encapsulation efficiency determination: the determination was performed with the reference to a Quant-iT RiboGreen RNA kit standard procedure.

TABLE 10
Characterization data of LNP prepared from
representative amino lipid compounds
	Serial			Encapsulation
Serial	number of	Z-Average		efficiency
number	amino lipid	(d · nm)	PDI	(%)
LNP-48	E3C116A11	102.4	0.10	88.9
LNP-49	E5C109A9	115.7	0.06	91.8
LNP-50	E5C114A9	116.3	0.13	95.9
LNP-51	E5C115A9	118.4	0.06	97.2
LNP-52	E5C128A9	114.2	0.13	93.9
LNP-53	E5C114A11	116.0	0.10	95.4
LNP-54	E5C115A11	116.9	0.07	97.5
LNP-55	E5C116A11	108.3	0.06	94.8
LNP-56	E7C114A9	124.6	0.12	94.9
LNP-57	E7C115A9	117.9	0.08	95.5
LNP-58	E7C123A9	118.2	0.08	92.4
LNP-59	E7C114A11	104.5	0.04	94.3
LNP-60	E7C115A11	112.7	0.06	95.5
LNP-61	E7C116A11	105.6	0.08	93.6
LNP-62	E7C126A11	109.6	0.2	90.2
LNP-63	E8C114A9	114.0	0.07	94.0
LNP-64	E8C115A9	119.1	0.10	95.3
LNP-65	E8C114A11	113.7	0.07	93.1
LNP-66	E8C115A11	114.6	0.10	95.6
LNP-67	E8C116A11	107.2	0.11	93.5
LNP-68	E8C123A11	115.4	0.04	94.9
LNP-69	E9C104A9	119.9	0.06	92.3
LNP-70	E9C114A9	125.1	0.08	91.5
LNP-71	E9C115A9	116.9	0.04	94.2
LNP-72	E9C114A11	105.5	0.06	96.1
LNP-73	E9C115A11	123.3	0.11	90.3
LNP-74	E9C116A11	110.8	0.05	94.9
LNP-75	E9C123A11	124.5	0.09	93.7
LNP-76	E10C114A9	121.6	0.09	94.6
LNP-77	E10C115A9	117.2	0.07	95.9
LNP-78	E10C114A11	115.9	0.13	93.7
LNP-79	E10C115A11	113.2	0.09	96.2
LNP-80	E11C109A9	119.6	0.05	93.5
LNP-81	E11C114A9	121.0	0.04	92.0
LNP-82	E11C115A9	126.4	0.06	94.8
LNP-83	E11C109A11	119.6	0.03	95.1
LNP-84	E11C114A11	124.1	0.08	96.6
LNP-85	E11C115A11	116.7	0.11	94.5
LNP-86	DLin-MC3	139.2	0.15	94.6
LNP-87	E7C115A11	102.5	0.05	93.4
LNP-88	E7C115A11	119.9	0.06	92.3
LNP-89	E7C115A11	128.8	0.15	94.2
LNP-90	E7C115A11	124.5	0.09	93.7
LNP-91	E7C115A11	131.4	0.09	92.6

• • Note: in the above table:
  • a lipid formulation of LNP-48 to LNP-86 was as follows: amino lipid:DSPC:cholesterol:PEG2000-DMG=47.5:10:41:1.5;
  • a lipid formulation of LNP-87 was as follows: amino lipid:DOPE:cholesterol:PEG2000-DMG=45:10:43.5:1.5;
  • a lipid formulation of LNP-88 was as follows: amino lipid:DSPC:DOPS:cholesterol:PEG2000-DMG=50:10:5:38.5:1.5;
  • a lipid formulation of LNP-89 was as follows: amino lipid:DSPC:DOPC:cholesterol:PEG2000-DMG=45:10:5:38.0:2.0;
  • a lipid formulation of LNP-90 was as follows: amino lipid:DSPC:DOPE:cholesterol:PEG2000-DSPE=50:10:5:33.5:1.5; and
  • a lipid formulation of LNP-91 was as follows: amino lipid:cholesterol:PEG2000-DSPE=60:35.5:4.5.

2. Animal Tests

• • Animal preparation: 6-week-old female C57BL/6 mice with the body weight about 20 g were selected. The feeding environment was an SPF stage feeding room. Animal tests were strictly performed according to the guide of the national health institute and the animal ethics requirements.
  • In-vivo delivery: 9 C57BL/6 mice were randomly selected for each group. According to an mRNA consumption of 0.5 mg/kg, a lipid nanoparticle solution was respectively injected in three administration manners of subcutaneous, intramuscular and tail intravenous injection (3 mice for each administration manner). After 12 h, 200 μL of 10 mg/mL D-luciferin potassium salt was injected into each mice through tail intravenous injection. After 10 min, the mice were placed in an in-vivo living imaging system (IVIS-200, Xenogen), the total fluorescence intensity of each mice was observed, and photos were taken for recording. The expression intensity of the Fluc mRNA delivered by the representative amino lipid compounds in 3 administration manners was as shown in Table 11 to Table 13. DLin-MC3 was used as a control.

TABLE 11
Expression intensity of Luc mRNA delivered by LNP subcutaneous
administration of representative amino lipid compounds
Serial	Serial number	Average fluorescence
number	of LNP	intensity
1	LNP-48	8.8E+06
2	LNP-52	5.1E+07
3	LNP-54	1.2E+08
4	LNP-56	5.1E+07
5	LNP-59	4.6E+07
6	LNP-60	1.1E+08
7	LNP-62	3.2E+07
8	LNP-65	8.3E+07
9	LNP-66	9.4E+07
10	LNP-69	2.6E+07
11	LNP-70	6.2E+07
12	LNP-72	8.8E+07
13	LNP-73	5.6E+07
14	LNP-76	6.7E+07
15	LNP-79	8.1E+07
16	LNP-82	9.3E+07
17	LNP-83	5.5E+07
18	LNP-85	7.2E+07
19	LNP-87	1.0E+08
20	LNP-90	8.9E+07

TABLE 12
Expression intensity of Luc mRNA delivered by
LNP intramuscular injection administration delivery
of representative amino lipid compounds
Serial	Serial number	Average fluorescence
number	of LNP	intensity
1	LNP-49	5.3E+07
2	LNP-54	1.5E+08
3	LNP-55	6.4E+07
4	LNP-59	7.0E+07
5	LNP-60	1.7E+08
6	LNP-61	6.1E+07
7	LNP-65	1.3E+08
8	LNP-66	9.9E+07
9	LNP-68	8.6E+07
10	LNP-70	8.2E+07
11	LNP-72	7.8E+07
12	LNP-73	6.4E+07
13	LNP-77	4.5E+07
14	LNP-80	4.8E+07
15	LNP-81	5.9E+07
16	LNP-83	6.4E+07
17	LNP-84	8.3E+07
18	LNP-85	6.8E+07
19	LNP-88	1.0E+08
20	LNP-90	9.6E+07

TABLE 13
Expression intensity of Luc mRNA delivered by LNP tail intravenous
administration delivery of representative amino lipid compounds
Serial	Serial number	Average fluorescence
number	of LNP	intensity
1	LNP-51	3.5E+07
2	LNP-53	3.3E+07
3	LNP-54	8.1E+07
4	LNP-56	6.2E+07
5	LNP-58	4.7E+07
6	LNP-59	7.9E+07
7	LNP-60	1.3E+08
8	LNP-64	5.4E+07
9	LNP-65	7.6E+07
10	LNP-66	8.9E+07
11	LNP-70	5.4E+07
12	LNP-72	4.9E+07
13	LNP-74	2.4E+07
14	LNP-78	3.8E+07
15	LNP-82	2.3E+07
16	LNP-83	9.9E+06
17	LNP-85	1.4E+07
18	LNP-87	1.0E+08
19	LNP-89	8.7E+07
20	LNP-91	6.3E+06

Embodiment 23: In-Vivo Immunity and Tumor Treatment Effect Evaluation of Lipid Nanoparticles Prepared from Amino Lipid Compounds

• • Preparation method: the amino lipid compounds of the disclosure, DSPC, cholesterol and PEG2000-DMG were mixed according to a mole ratio of 50:10:38.5:1.5 in absolute ethyl alcohol. OVA mRNA was dissolved in a sodium acetate buffer solution (50 nM, pH=4.0). The ratio of the ethyl alcohol solution to the acetate buffer solution (50 nM, pH=4.0) was controlled to be 1:3 by using two micro-injection pumps to prepare a coarse solution of the lipid nanoparticles in a micro-fluidic chip. Then, dialysis was performed for 6 h under the conditions of 1×PBS and temperature control at 4° C. by using a dialysis box (Fisher, MWCO 20,000). Before use, filtration was performed by a 0.22 μm microporous filtering membrane. A mass ratio of the amino lipid compound to OVA mRNA was about 8:1.
  • Animal preparation: 5-6-week-old female C57BL/6 mice with the body weight about 18 to 20 g were selected. The feeding environment was an SPF stage feeding room. Animal tests were strictly performed according to the guide of the national health institute and the animal ethics requirements.
  • In-vivo delivery: B16-OVA melanoma cells (1.5×10⁵) were injected to the outer thighs of the mice through subcutaneous injection. When the tumor grew to 50 mm³ (on the about 6^th or 7^th day after tumor inoculation), the mice began to inoculated vaccines, the animals were subjected to twice immunization through intramuscular injection of an LNP preparation containing 5 μg OVA-mRNA. An interval between first and second injections was 7 days. The tumor growth was measured for 3 times every week by a digital display calliper, and a calculation formula was 0.5×length×width. When the tumor size reached 1500 mm³, the mice received euthanasia. The tumor growth speed of E7C₁₁₅A11 was obviously lower than that of MC3 group, 90% of the mice tumors completely disappeared (as shown in FIG. 5), and the survival rate reached 100%, and was obviously superior to that of MC3 group (as shown in FIG. 6).

Embodiment 24: 2-((6-(dimethylamino)hexanoyl)oxy)dodecyl 2-hexyldecanoate

EDC·HCl (192 mg, 1 mmol), DIPEA (174 μL, 1 mmol), DMAP (3.0 mg, 0.025 mmol), 6-(dimethylamino)hexanoic acid (96 mg, 0.6 mmol), 2-hydroxydodecyl 2-hexyldecanoate (220 mg, 0.5 mmol) and DCM (4 mL) were sequentially added into a 10 mL reaction tube, and the reaction was stirred at room temperature for 3 h to obtain the compound E7C71A12 (216 mg, 74%). ¹H NMR (400 MHz, CDCl₃): δ 0.86 (t, J=6.5 Hz, 9H), 1.24-1.46 (m, 40H), 1.54-1.68 (m, 8H), 2.27-2.34 (m, 9H), 2.40-2.44 (m, 2H), 4.02 (dd, J₁=11.7 Hz, J₂=6.1 Hz, 1H), 4.23 (dd, J₁=12.2 Hz, J₂=3.5 Hz, 1H), 5.03-5.06 (m, 1H). ¹³C NMR (100 MHz, CDCl₃): δ 14.1, 22.7, 24.7, 25.3, 26.7, 27.4, 29.3, 29.4, 29.6, 30.7, 31.8, 31.9, 32.7, 34.2, 44.7, 47.0, 61.6, 65.8, 70.7, 173.1, 175.8. ESI-MS calculated for C₃₆H₇₂NO₄⁺ [M+H]⁺ 582.5, found 582.5.

The compound E7C71A9 was synthesized according to the procedures described in embodiments 4 and 5 of this patent, while Compound A, Compound B, and Compound C were prepared by reference to the methods reported in the previously published patents. Take the synthesis of Compound A as an example:

The construction of tridecane-1,3-diol (CAS number: 39516-29-5) was conducted in two steps using 3-(tert-butyldimethylsilyloxy)-propanal (CAS number: 89922-82-7) as the starting material, referring to the method reported in the published patent (U.S. Ser. No. 11/013,696B2).

Embodiment 25: 3-hydroxytridecyl 2-hexyldecanoate

Tridecane-1,3-diol (1.08 g, 5 mmol), 2-hexyldecanoic acid (1.54 g, 6 mmol), EDC·HCl (1.35 g, 7 mmol), DMAP (245 mg, 2 mmol), DIPEA (647 mg, 5 mmol) and DCM (20 mL) were added into a 50 mL round bottom flask charged with a magnetic stirring bar, then the resultant mixture was stirred at room temperature for 12 h. The product 3-hydroxytridecyl 2-hexyldecanoate (1.30 g, 57% yield) was obtained by column chromatography on silica gel through gradient elution purification (hexane:EA=20:1 to 10:1). ¹H NMR (400 MHz, CDCl₃): δ 0.88 (t, 9H), 1.23-1.27 (m, 36H), 1.38-1.40 (m, 2H), 1.61-1.64 (m, 4H), 1.79-1.82 (m, 2H), 2.11-2.12 (m, 1H), 3.38-3.40 (m, 1H), 4.06 (t, 2H), 4.49 (brs, 1H). ESI-MS calculated for C₂₉Hs903 [M+H]⁺ 455.4, found 455.5.

Embodiment 26: 3-((4-(dimethylamino)butanoyl)oxy)tridecyl 2-hexyldecanoate (compound A)

EDC·HCl (192 mg, 1 mmol), DIPEA (174 μL, 1 mmol), DMAP (3.0 mg, 0.025 mmol), 3-hydroxytridecyl 2-hexyldecanoate (228 mg, 0.5 mmol), 4-(dimethylamino)butanoic acid (101 mg, 0.6 mmol) and DCM (4 mL) were sequentially added into a 10 mL reaction tube, then reaction was stirred at room temperature for 3 h. The product 3-((4-(dimethylamino)butanoyl)oxy)tridecyl 2-hexyldecanoate (compound A, 227 mg, 80% yield) was obtained by column chromatography on silica gel through gradient elution purification (MeOH:DCM=1:99 to 3:97). ¹H NMR (400 MHz, CDCl₃): δ 0.88 (t, 9H), 1.22-1.28 (m, 36H), 1.48-1.49 (m, 2H), 1.58-1.61 (m, 4H), 1.86-1.88 (m, 4H), 2.03 (s, 6H), 2.13-2.14 (m, 1H), 2.35 (t, 2H), 3.04 (t, 2H), 4.06 (t, 2H), 4.46-4.47 (m, 1H). ¹³C NMR (100 MHz, CDCl₃): δ 14.1, 22.7, 23.4, 25.6, 29.3, 29.4, 31.8, 31.9, 32.7, 33.3, 34.2, 44.7, 47.0, 61.0, 61.4, 71.2, 173.1, 175.8. ESI-MS calculated for C₃₅H₇₀NO₄⁺ [M+H]⁺ 568.5, found 568.9.

Embodiment 27: In-Vitro Evaluation of Amino Lipid Compounds (E7C71A9, Compound a, Compound B, Compound C) as mRNA Vector

• • Cell line: HeLa cell line (ATCC)
  • Culture medium: 1640 (Invitrogen) supplemented with 10% fetal calf serum
  • Screening form: 96-well plate cell transfection
  • Detection: fluorescence intensity detection by a multifunctional microplate reader. According to manufacturer's instructions, Lipofectamine3000 (Invitrogen) was used as a positive control group.
  • Method: a pipette was used for sample addition. The shown content is the content of a single well of a 96-well plate.
  • 1. Synthesis of E7C71A9, Compound A, Compound B, and Compound C was performed as described above. And these four ionizable amino lipid compounds were respectively mixed with DSPC, cholesterol and PEG2000-DMG into absolute ethyl alcohol. according to a mole ratio of 43:10.5:45:1.5, The Luc-mRNA (TriLink) was dissolved into a sodium acetate buffer solution (25 nM, pH=5.0). The mixed lipid solution was taken out by a pipette, and was added into the Luc-mRNA solution to be sufficiently mixed. A proportion ratio of the ethyl alcohol solution to the sodium acetate buffer solution (25 nM, pH=5.0) was controlled to be 1:3, and a nanoparticle solution was prepared. A mass ratio of the amino lipid compound to luciferase mRNA (Luc mRNA) was about 10:1, and the mRNA consumption in each well was 100 ng.
  • 2. After the lipid nanoparticle solution was incubated for 30 min at room temperature, 100 μL of fresh resuspended HeLa cells (1×10⁴ cells) were added into each well of a 96-well all-white ELISA plate. Then, the lipid nanoparticle solution was added into the 96-well plate (10 μL for each well) by a pipette. The solution was placed into a 37° C. incubator containing 5% CO₂ to be incubated.
  • 3. After 16 h to 20 h of cell initial transfection, a substrate ONE-Glo™ Luciferase was added into cells at 100 μL/well, and after 2 min, detection was performed by a multifunctional microplate reader (Biorek SynergyH1).
  • 4. The relative transfection efficiency was calculated as follows:

Relative transfection efficiency (%)=fluorescence intensity of LNP/fluorescence intensity of Lip3000×100%.

• • Result: the transfection efficiency of parts of compounds on Luc-mRNA of the HeLa cells is shown in Table 14.

TABLE 14
relative transfection efficiency of the above
four compounds on Luc-mRNA of the HeLa cells
Serial Number of Amino Lipid Relative Transfection Efficiency
E7C71A9                      2.0
Compound A                   0.62
Compound B                   0.45
Compound C                   0.39
Lip3000                      1

Embodiment 28: Transfection of Lipid Nanoparticles Prepared from the Above Four Amino Compounds on BMDC Primary Cells

• • Preparation method: the same as that described above.
  • Animal preparation: 6-week-old female C₅₇BL/6 mice with the body weight about 20 g were selected. The feeding environment was an SPF stage feeding room. Animal tests were strictly performed according to the guide of the national health institute and the animal ethics requirements.
  • Cell acquisition: C57BL/6 mice were killed through cervical dislocation, and were soaked for 5 min in 75% ethyl alcohol. Dissection was performed to obtain thigh and calf tibiae of the mice. Attached muscles were removed to expose sclerotin. Then, bone marrow in tibiae was blown out by using a 1 mL injector sucked with PBS. After the bone marrow was blown away, impurities were filtered away by a 50 μm filter screen. A red blood cell lysis buffer (3-4 mL) was added into an obtained filtrate. Then, after placement for 5 min, 800 g centrifugation was performed for 5 min to remove the supernatant. The obtained cells were placed into a 1640 culture medium (containing 10% fetal calf serum, 20 ng/mL GMCSF and 10 ng/mL IL4) to be resuspended, and were inoculated into a 6-well plate at an inoculation density of 100000 cells/ml culture medium. The materials were placed into a 37° C. cell incubator containing 5% CO₂. Half liquid change was performed once every 2 days. Suspended cells and loose wall attached cells were collected on the seventh day, and were inoculated to a 96-well all-white ELISA plate at the inoculation density of 20000 cells per well, and the volume of a culture medium was 100 μL.
  • Cell transfection: lipid nanoparticles coated with luciferase mRNA were added into a 96-well all-white ELISA plate laid with primary cells. The adding volume of the mRNA lipid nanoparticles in each well was controlled to be 10 μL. Then, the materials were put into a 37° C. incubator containing 5% CO₂ for 16 h.
  • Transfection efficiency detection: 20 μL of a substrate ONE-Glo™ Luciferase was added into each well of a 96-well all-white ELISA plate. After 1 min, detection was performed by a multifunctional microplate reader (Biorek SynergyH1). The expression intensity of the LucmRNA transfection of representative amino lipid compounds on BMDC was as shown in Table 15. DLin-MC3 was used as a control.

TABLE 15
Transfection expression intensity of the
above four amino lipid compounds on BMDC
Serial Number of Amino Lipid Fluorescence intensity
E7C71A9                      8.8E+04
Compound A                   4.9E+04
Compound B                   2.5E+04
Compound C                   9.3E+03

The above descriptions are only exemplary embodiments of the disclosure, and are not intended to limit the disclosure. All changes, equivalents, improvements, etc. made within the spirit and principle of the disclosure all fall within the protection scope of the disclosure.

Claims

1. An amino lipids with the structure as shown in Formula (I):

2. The amino lipid according to claim 1, wherein the R1 is selected from C4-C17 alkyl, C4-C17 alkenyl, C4-C17 alkynyl, C4-C17 cycloalkyl, C4-C17 cycloalkenyl and C4-C17 cycloalkynyl; the C4-C17 alkyl, C4-C17 alkenyl, C4-C17 alkynyl, C4-C17 cycloalkyl, C4-C17 cycloalkenyl and C4-C17 cycloalkynyl are able to be optionally substituted by H, C1-C6 hydrocarbyl and F; and/or the R2 is selected from C5-C19 alkyl, C5-C19 alkenyl, C5-C19 alkynyl, C5-C19 cycloalkyl, C5-C19 cycloalkenyl and C5-C19 cycloalkynyl; the C5-C19 alkyl, C5-C19 alkenyl, C5-C19 alkynyl, C5-C10 cycloalkyl, C5-C19 cycloalkenyl and C5-C19 cycloalkynyl are able to be optionally substituted by H, C1-C6 hydrocarbyl and F; and/orR3, R4 and L form an R3R4—N-L amine-containing carboxylic acid structure of

3. The amino lipid according to claim 2, wherein the R1 is selected from C4-C17 alkyl and C4-C17 alkenyl; the C4-C17 alkyl and C4-C17 alkenyl are able to be optionally substituted by H, C1-C6 hydrocarbyl and F; and/or the R2 is selected from C5-C19 alkyl, C5-C19 alkenyl and C5-C19 cycloalkyl; and the C5-C19 alkyl, C5-C19 alkenyl and C5-C19 cycloalkyl are able to be optionally substituted by H, C1-C6 hydrocarbyl and F.

4. The amino lipid according to claim 3, wherein a structure is as shown in Formula (II):

5. The amino lipid according to claim 3, wherein the R1 is selected from C4-C17 alkyl; the C4-C17 alkyl are able to be optionally substituted by H, C1-C6 hydrocarbyl and F; and/or the R2 is selected from C5-C19 alkenyl and C5-C19 cycloalkyl; and the C5-C19 alkenyl and C5-C19 cycloalkyl are able to be optionally substituted by H, C1-C6 hydrocarbyl and F.

6. The amino lipid according to claim 3, wherein the R1 is selected from C4-C17 alkenyl; the C4-C17 alkenyl is able to be optionally substituted by H, C1-C6 hydrocarbyl and F; and/or the R2 is selected from C5-C19 alkyl and C5-C19 cycloalkyl; and the C5-C19 alkyl and C5-C19 cycloalkyl are able to be optionally substituted by H, C1-C6 hydrocarbyl and F.

7. The amino lipid according to claim 2, wherein the R1 is one selected from E1, E2, E3, E4, E5, E6, E7, E8, E9, E10, E11, E12, E13, E14, E15, E16, E17, E18, E19, E20, E21, E22, E23, E24 and E25:

8. The amino lipid according to claim 3, wherein the R1 is one selected from E3, E4, E5, E6, E7, E8, E9, E10, E11, E12, E13, E15, E17, E18, E20, E21 and E24; and/or the R2 is one selected from C3, C4, C5, C6, C7, C8, C9, C10, C11, C12, C13, C14, C56, C57, C58, C60, C62, C63, C64, C66, C67, C71, C72, C74, C79, C82, C83, C102, C103, C104, C105, C106, C107, C108, C109, C110, C111, C112, C113, C114, C115, C116, C117, C118, C119, C120, C121, C122, C123, C124, C125, C126, C127, C128, C129, C130, C131, C132, C133, C134, C135, C136, C137, C138, C139, C140, C141, C142, C143, C144, C145, C146, C147; and/or

9. The amino lipid according to claim 1, being one selected from compounds of the following structures.

10. A preparation method of amino lipid, comprising the following steps: S1: taking a solvent-free reaction of compound R2COOH with an epoxide compound under the catalysis of FeCl3 and Py,wherein the structure of the epoxide compound is described as follows:

11. An application of the amino lipid according to claim 1 or a pharmaceutically acceptable salt thereof in the preparation of drugs for gene therapy, genetic vaccination, antisense therapy or interfering RNA therapy.

12. The application according to claim 11, wherein the drugs are used for treating cancer, genetic disease, allergy, toxicity or pathogen infection diseases.

13. The application according to claim 11, wherein the cancer is lung cancer, gastric cancer, liver cancer, esophagus cancer, colorectal cancer, pancreatic cancer, cerebral cancer, lymph cancer, leukemia, bladder cancer or prostatic cancer.

14. A nucleic acid administration system, wherein raw materials of the nucleic acid administration system comprise the amino lipid according to claim 1.

15. A composition formed by the amino lipid according to claim 1, wherein the composition and a nucleic acid drug form a drug preparation, and the nucleic acid drug comprises DNA and RNA.

16. The composition according to claim 15, wherein the composition comprises 30-50% of an amino lipid, 40-52% of a structure lipid, 5-20% of an auxiliary lipid and 0.5-5% of a PEG lipid, wherein a total molar content of the four above ingredients is 100%; and a mass ratio of the amino lipid to the nucleic acid is 1:1-50:1.

17. The composition according to claim 16, wherein the structure lipid comprises cholesterol and its derivative, preferably comprising cholesterol.

18. The composition according to claim 16, wherein the auxiliary lipid comprises DSPC, DSPE, DOPE, DOPC and DOPS, preferably comprising DSPC and DOPE, and more preferably comprising DSPC.

19. The composition according to claim 16, wherein the PEG lipid comprises PEG-DMG and PEG-DSPE, and preferably comprising PEG-DMG.

20. The composition according to claim 16, wherein its administration manners comprise aerosolization administration, intravenous injection, subcutaneous injection, intramuscular injection and ophthalmic administration, and preferably intravenous injection, subcutaneous injection and intramuscular injection.