Patent Application
20090004185
The present invention relates to amino-substituted quinazoline derivatives as inhibitors of β-catenin/tcf-4 pathway, which can be useful in the treatment of cancer; to processes for their preparation; to pharmaceutical compositions comprising them; and to methods of using them.
Colorectal cancer is the second leading cause of cancer deaths in the United States. Most (85%) colorectal cancers have loss or mutation of tumor suppressor gene Adenomatous Polyposis Coli (APC) which initiates a neoplastic process towards carcinoma formation. APC, along with β-catenin, is a central component of Wnt signaling pathway. The name Wnt was coined as a combination of Wg (wingless) and Int. The wingless gene had originally been identified as a segment polarity gene in Drosophila melanogaster that functions during embryogenesis and also during adult limb formation during metamorphosis. The Int genes were originally identified as vertebrate genes near several integration sites of mouse mammary tumor virus (MMTV). The Int-1 gene and the wingless gene were found to be homologous, with a common evolutionary origin evidenced by similar amino acid sequences of their encoded proteins. Mutations of the wingless gene in the fruit fly were found in wingless flies, while tumors caused by MMTV were found to have copies of the virus integrated into the genome forcing overproduction of one of several Wnt genes. Wnts are a major class of secreted morphogenic ligands of profound importance in establishing the pattern of development in the bodies of all multicellular organisms studied.
Wnt signaling pathway is evolutionally conserved in mammalians, Xenopus, Drosophila and C. elegans. It controls many events during embryonic development and regulates proliferation, morphology, motility and cell fate at a cellular level. Within the pathway, APC in complex with Axin is required to regulate the stability of β-catenin. In the absence of secreted Wnt factors, cytoplasmic β-catenin is phosphorylated by GSK3β kinase in the APC complex and is later subject to rapid protein degradation. In the presence of Wnt factors, the Wnt signaling cascade is activated so that the intrinsic kinase activity of the APC complex is inhibited. As a consequence, stable non-phosphorylated β-catenin accumulates in the cytoplasm and subsequently translocates into nucleus, where it binds to Tcf-4 (T-cell transcriptional factor-4) protein and activates the transcription of a variety of Wnt target genes. Numerous candidate genes have been proposed as critical downstream effectors of Wnt signaling in cancer, including c-myc, cyclin D1, BMP4, KLF4, DHRS9/DHRL, MDR-1, Axin2, GPR49, ROR1, TIMP2, ID2, MSX1, and CSF2.
Therefore, loss of functional APC leads to inappropriate stabilization of β-catenin. In addition, β-catenin can be activated by intragenic mutations that abolish inhibitory phosphorylation sites so that β-catenin is no longer degraded. Activating mutations in β-catenin can occasionally replace inactivating mutations of APC in the initiation of sporadic colorectal cancer (2 to 5% of all colon tumors). Both mutations result in accumulation of non-phosphorylated β-catenin thereby constitutively activating gene transcription and probably promoting carcinogenesis. Introduction of wild type APC into cells which have lost APC function has been shown to result in either growth suppression or apoptosis, suggesting that these cells have become dependent on elevated β-catenin/Tcf-4 signaling.
Accordingly, inhibitors of β-catenin/Tcf-4 pathway can be useful for the treatment of cancer, especially, for the treatment of colorectal cancer.
In one aspect, the present invention provides a compound of formula I,
or an enantiomer, diastereomer, tautomer, or pharmaceutically acceptable salt or solvate thereof, wherein the symbols have the following meanings and are, for each occurrence, independently selected:
In certain embodiments, R1, R2, R3, and R4 of the compound of formula I are each independently hydrogen, halogen, cyano, nitro, CF3, OCF3, C1-C4 alkyl or substituted C1-C4 alkyl, C2-C6 alkenyl or substituted C2-C6 alkenyl, C2-C6 alkynyl or substituted C2-C6 alkynyl, cycloalkyl or substituted cycloalkyl, cycloalkenyl or substituted cycloalkenyl, heterocyclyl or substituted heterocyclyl, aryl or substituted aryl, ORa, SRa, S(═O)Re, S(═O)2Re, S(═O)2ORe, NRbRc, NRbS(═O)2Re, S(═O)2NRbRc, C(═O)ORe, C(═O)Ra, C(═O)NRbRc, OC(═O)Ra, OC(═O)NRbRc, NRbC(═O)ORe, NRdC(═O)NRbRc, NRdS(═O)2NRbRc, or NRbC(═O)Ra.
In certain other embodiments, R2 and R3 of the compound of formula I are each independently hydrogen, halogen, cyano, nitro, CF3, OCF3, C1-C4 alkyl or substituted C1-C4 alkyl, C2-C6 alkenyl or substituted C2-C6 alkenyl, C2-C6 alkynyl or substituted C2-C6 alkynyl, C3-C7 cycloalkyl or substituted C3-C7 cycloalkyl, heterocyclyl or substituted heterocyclyl, aryl or substituted aryl, ORa, C(═O)ORe, or C(═O)Ra.
In certain embodiments, R6 and R7 of the compound of formula I are each independently hydrogen, C1-C4 alkyl or substituted C1-C4 alkyl, C3-C7 cycloalkyl or substituted C3-C7 cycloalkyl, or said R6 and R7 together with the N to which they are bonded optionally form a heterocycle or substituted heterocycle, in which said heterocycle is fully saturated or partially unsaturated.
In certain other embodiments, R5 of the compound of formula I is hydrogen or methyl. In some embodiments, R14 of the compound of formula I is heteroaryl or substituted heteroaryl. In some other embodiments, R14 of the compound of formula I is heterocycle or substituted heterocycle, in which said heterocycle is fully saturated. In certain embodiments, R14 of the compound of formula I is phenyl or substituted phenyl. In certain other embodiments, R14 of the compound of formula I is pyridinyl or substituted pyridinyl.
In certain embodiments, R14 of the compound of formula I is piperidinyl or substituted piperidinyl. In certain other embodiments, R14 of the compound of formula I is:
wherein R15 is hydrogen, C1-C4 alkyl, C3-C7 cycloalkyl, —CH2-phenyl or —CH2-substituted phenyl, or —CH2-heteroaryl or —CH2-substituted heteroaryl. In some embodiments, R14 of the compound of formula I is —NH-aryl or —NH-substituted aryl. In some other embodiments, R14 of the compound of formula I is —NH-phenyl or —NH-substituted phenyl.
In another aspect, the present invention provides a compound of formula I, or an enantiomer, diastereomer, tautomer, or pharmaceutically acceptable salt or solvate thereof, wherein the compound of formula I is selected from the group consisting of:
In yet another aspect, the present invention provides a compound of formula I, or an enantiomer, diastereomer, tautomer, or pharmaceutically acceptable salt or solvate thereof, wherein the compound of formula I is selected from the group consisting of:
In yet another aspect, the present invention provides a compound of formula I, or an enantiomer, diastereomer, tautomer, or pharmaceutically acceptable salt or solvate thereof, wherein the compound of formula I is selected from the group consisting of:
In yet another aspect, the present invention provides a compound of formula I, or an enantiomer, diastereomer, tautomer, or pharmaceutically acceptable salt or solvate thereof, wherein the compound of formula I is selected from the group consisting of:
In yet another aspect, the present invention provides a compound of formula I, or an enantiomer, diastereomer, tautomer, or pharmaceutically acceptable salt or solvate thereof, wherein the compound of formula I is selected from the group consisting of:
In a further aspect, the present invention provides a pharmaceutical composition comprising at least one compound as described hereinabove, and a pharmaceutically-acceptable carrier or diluent. In certain embodiments, the pharmaceutical composition of the present invention may further comprise at least one other anti-cancer agent or cytotoxic agent. In some embodiments, the other anti-cancer or cytotoxic agent is selected from the group consisting of 5-FU, leucovorin, irinotecan, bevacizumab, cetuximab, intraarterial floxuridine, oxaliplatin, gefitinib, and fluorouracil.
In another aspect, the present invention provides a method of inhibiting beta-catenin/Tcf-4 pathway comprising administering to a mammalian species in need thereof an effective amount of at least one compound as described hereinabove.
In yet another aspect, the present invention provides a method for treating a condition or disorder comprising administering to a mammalian species in need thereof a therapeutically effective amount of at least one compound as described hereinabove, wherein the condition or disorder is selected from the group consisting of proliferate diseases and cancers. In certain embodiments, the condition or disorder is colorectal cancer. The invention also includes use of a compound of the invention in the manufacture of a medicament for the treatment of a condition or disorder is selected from the group consisting of proliferate diseases and cancers, preferably colorectal cancer.
In yet another aspect, the present invention provides a method for treating a condition or disorder comprising administering to a mammalian species in need thereof a therapeutically effective amount of at least one compound as described hereinabove, in combination with at least one other anti-cancer or cytotoxic agent. In certain embodiments, said other anti-cancer or cytotoxic agent is selected from the group consisting of 5-FU, leucovorin, irinotecan, bevacizumab, cetuximab, intraarterial floxuridine, oxaliplatin, gefitinib, and fluorouracil.
In a further aspect, the present invention provides a method of inhibiting the transcription of a gene selected from the group consisting of c-myc, cyclin D1, BMP4, KLF4, DHRS9/DHRL, MDR-1, Axin2, GPR49, ROR1, TIMP2, ID2, MSX1, and CSF2, comprising administering to a mammalian species in need thereof an effective amount of at least one compound as described hereinabove.
In another aspect, the present invention provides a method for making a compound of formula I,
or an enantiomer, diastereomer, tautomer, or pharmaceutically acceptable salt or solvate thereof, comprising:
(a) reacting a compound of formula II,
with an amine of formula III,
to provide a compound of formula IV; and
(b) further reacting the compound of formula IV with an amine of formula HNR6R7 to give the compound of formula I;
wherein the symbols of each of the above formulae have the following meanings and are, for each occurrence, independently selected:
In yet another aspect, the present invention provides a method for making a compound of formula I,
or an enantiomer, diastereomer, tautomer, or pharmaceutically acceptable salt or solvate thereof, comprising:
(a) reacting a compound of formula II,
with an amine of formula V,
to provide a compound of formula VI;
(b) reacting the compound of formula VI with an acid chloride of formula R14(C═O)Cl, or an acid of formula of R14C(═O)OH, to give a compound of formula IV; and
(c) further reacting the compound of formula IV with an amine of formula HNR6R7 to the compound of formula I;
wherein the symbols of each of the above formulae have the following meanings and are, for each occurrence, independently selected:
In yet another aspect, the present invention provides a compound of formula I prepared according to the methods as described hereinabove.
The following are definitions of terms used in the present specification. The initial definition provided for a group or term herein applies to that group or term throughout the present specification individually or as part of another group, unless otherwise indicated.
The terms “alkyl” and “alk” refers to a straight or branched chain alkane (hydrocarbon) radical containing from 1 to 12 carbon atoms, preferably 1 to 6 carbon atoms. Exemplary “alkyl” groups include methyl, ethyl, propyl, isopropyl, n-butyl, t-butyl, isobutyl pentyl, hexyl, isohexyl, heptyl, 4,4-dimethylpentyl, octyl, 2,2,4-trimethylpentyl, nonyl, decyl, undecyl, dodecyl, and the like. The term “C1-C4 alkyl” refers to a straight or branched chain alkane (hydrocarbon) radical containing from 1 to 4 carbon atoms, such as methyl, ethyl, propyl, isopropyl, n-butyl, t-butyl, and isobutyl. “Substituted alkyl” refers to an alkyl group substituted with one or more substituents, preferably 1 to 4 substituents, at any available point of attachment. Exemplary substituents include but are not limited to one or more of the following groups: hydrogen, halogen (e.g., a single halogen substituent or multiple halo substitutents forming, in the latter case, groups such as CF3 or an alkyl group bearing Cl3), cyano, nitro, oxo (i.e., ═O), CF3, OCF3, cycloalkyl, alkenyl, cycloalkenyl, alkynyl, heterocycle, aryl, ORa, SRa, S(═O)Re, S(═O)2Re, P(═O)2Re, S(═O)2ORe, P(═O)2ORe, NRbRc, NRbS(═O)2Re, NRbP(═O)2Re, S(═O)2NRbRc, P(═O)2NRbRc, C(═O)ORd, C(═O)Ra, C(═O)NRbRc, OC(═O)Ra, OC(═O)NRbRc, NRbC(═O)ORe, NRdC(═O)NRbRc, NRdS(═O)2NRbRc, NRdP(═O)2NRbRc, NRbC(═O)Ra, or NRbP(═O)2Re, wherein each occurance of Ra is independently hydrogen, alkyl, cycloalkyl, alkenyl, cycloalkenyl, alkynyl, heterocycle, or aryl; each occurance of Rb, Rc and Rd is independently hydrogen, alkyl, cycloalkyl, heterocycle, aryl, or said Rb and Rc together with the N to which they are bonded optionally form a heterocycle; and each occurance of Re is independently alkyl, cycloalkyl, alkenyl, cycloalkenyl, alkynyl, heterocycle, or aryl. In the aforementioned exemplary substitutents, groups such as alkyl, cycloalkyl, alkenyl, alkynyl, cycloalkenyl, heterocycle and aryl can themselves be optionally substituted.
The term “alkenyl” refers to a straight or branched chain hydrocarbon radical containing from 2 to 12 carbon atoms and at least one carbon-carbon double bond. Exemplary such groups include ethenyl or allyl. “Substituted alkenyl” refers to an alkenyl group substituted with one or more substituents, preferably 1 to 4 substituents, at any available point of attachment. Exemplary substituents include but are not limited to one or more of the following groups: hydrogen, halogen (e.g., a single halogen substituent or multiple halo substitutents forming, in the latter case, groups such as CF3 or an alkyl group bearing Cl3), cyano, nitro, oxo (i.e., ═O), CF3, OCF3, cycloalkyl, alkenyl, cycloalkenyl, alkynyl, heterocycle, aryl, ORa, SRa, S(═O)Re, S(═O)2Re, P(═O)2Re, S(═O)2ORe, P(═O)2ORe, NRbRc, NRbS(═O)2Re, NRbP(═O)2Re, S(═O)2NRbRc, P(═O)2NRbRc, C(═O)ORd, C(═O)Ra, C(═O)NRbRc, OC(═O)Ra, OC(═O)NRbRc, NRbC(═O)ORe, NRdC(═O)NRbRc, NRdS(═O)2NRbRc, NRdP(═O)2NRbRc, NRbC(═O)Ra, or NRbP(═O)2Re, wherein each occurance of Ra is independently hydrogen, alkyl, cycloalkyl, alkenyl, cycloalkenyl, alkynyl, heterocycle, or aryl; each occurance of Rb, Rc and Rd is independently hydrogen, alkyl, cycloalkyl, heterocycle, aryl, or said Rb and Rc together with the N to which they are bonded optionally form a heterocycle; and each occurance of Re is independently alkyl, cycloalkyl, alkenyl, cycloalkenyl, alkynyl, heterocycle, or aryl. The exemplary substitutents can themselves be optionally substituted.
The term “alkynyl” refers to a straight or branched chain hydrocarbon radical containing from 2 to 12 carbon atoms and at least one carbon to carbon triple bond. Exemplary such groups include ethynyl. “Substituted alkynyl” refers to an alkynyl group substituted with one or more substituents, preferably 1 to 4 substituents, at any available point of attachment. Exemplary substituents include but are not limited to one or more of the following groups: hydrogen, halogen (e.g., a single halogen substituent or multiple halo substitutents forming, in the latter case, groups such as CF3 or an alkyl group bearing Cl3), cyano, nitro, oxo (i.e., ═O), CF3, OCF3, cycloalkyl, alkenyl, cycloalkenyl, alkynyl, heterocycle, aryl, ORa, SRa, S(═O)Re, S(═O)2Re, P(═O)2Re, S(═O)2ORe, P(═O)2ORe, NRbRc, NRbS(═O)2Re, NRbP(═O)2Re, S(═O)2NRbRc, P(═O)2NRbRc, C(═O)ORd, C(═O)Ra, C(═O)NRbRc, OC(═O)Ra, OC(═O)NRbRc, NRbC(═O)ORe, NRdC(═O)NRbRc, NRdS(═O)2NRbRc, NRdP(═O)2NRbRc, NRbC(═O)Ra, or NRbP(═O)2Re, wherein each occurance of Ra is independently hydrogen, alkyl, cycloalkyl, alkenyl, cycloalkenyl, alkynyl, heterocycle, or aryl; each occurance of Rb, Rc and Rd is independently hydrogen, alkyl, cycloalkyl, heterocycle, aryl, or said Rb and Rc together with the N to which they are bonded optionally form a heterocycle; and each occurance of Re is independently alkyl, cycloalkyl, alkenyl, cycloalkenyl, alkynyl, heterocycle, or aryl. The exemplary substitutents can themselves be optionally substituted.
The term “cycloalkyl” refers to a fully saturated cyclic hydrocarbon group containing from 1 to 4 rings and 3 to 8 carbons per ring. Exemplary such groups include cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, etc. “Substituted cycloalkyl” refers to a cycloalkyl group substituted with one or more substituents, preferably 1 to 4 substituents, at any available point of attachment. Exemplary substituents include but are not limited to one or more of the following groups: hydrogen, halogen (e.g., a single halogen substituent or multiple halo substitutents forming, in the latter case, groups such as CF3 or an alkyl group bearing Cl3), cyano, nitro, oxo (i.e., ═O), CF3, OCF3, cycloalkyl, alkenyl, cycloalkenyl, alkynyl, heterocycle, aryl, ORa, SRa, S(═O)Re, S(═O)2Re, P(═O)2Re, S(═O)2ORe, P(═O)2ORe, NRbRc, NRbS(═O)2Re, NRbP(═O)2Re, S(═O)2NRbRc, P(═O)2NRbRc, C(═O)ORd, C(═O)Ra, C(═O)NRbRc, OC(═O)Ra, OC(═O)NRbRc, NRbC(═O)ORe, NRdC(═O)NRbRc, NRdS(═O)2NRbRc, NRdP(═O)2NRbRc, NRbC(═O)Ra, or NRbP(═O)2Re, wherein each occurance of Ra is independently hydrogen, alkyl, cycloalkyl, alkenyl, cycloalkenyl, alkynyl, heterocycle, or aryl; each occurance of Rb, Rc and Rd is independently hydrogen, alkyl, cycloalkyl, heterocycle, aryl, or said Rb and Rc together with the N to which they are bonded optionally form a heterocycle; and each occurance of Re is independently alkyl, cycloalkyl, alkenyl, cycloalkenyl, alkynyl, heterocycle, or aryl. The exemplary substitutents can themselves be optionally substituted. Exemplary substituents also include spiro-attached or fused cyclic substituents, especially spiro-attached cycloalkyl, spiro-attached cycloalkenyl, spiro-attached heterocycle (excluding heteroaryl), fused cycloalkyl, fused cycloalkenyl, fused heterocycle, or fused aryl, where the aforementioned cycloalkyl, cycloalkenyl, heterocycle and aryl substitutents can themselves be optionally substituted.
The term “cycloalkenyl” refers to a partially unsaturated cyclic hydrocarbon group containing 1 to 4 rings and 3 to 8 carbons per ring. Exemplary such groups include cyclobutenyl, cyclopentenyl, cyclohexenyl, etc. “Substituted cycloalkenyl” refers to a cycloalkenyl group substituted with one more substituents, preferably 1 to 4 substituents, at any available point of attachment. Exemplary substituents include but are not limited to one or more of the following groups: hydrogen, halogen (e.g., a single halogen substituent or multiple halo substitutents forming, in the latter case, groups such as CF3 or an alkyl group bearing Cl3), cyano, nitro, oxo (i.e., ═O), CF3, OCF3, cycloalkyl, alkenyl, cycloalkenyl, alkynyl, heterocycle, aryl, ORa, SRa, S(═O)Re, S(═O)2Re, P(═O)2Re, S(═O)2ORe, P(═O)2ORe, NRbRc, NRbS(═O)2Re, NRbP(═O)2Re, S(═O)2NRbRc, P(═O)2NRbRc, C(═O)ORd, C(═O)Ra, C(═O)NRbRc, OC(═O)Ra, OC(═O)NRbRc, NRbC(═O)ORe, NRdC(═O)NRbRc, NRdS(═O)2NRbRc, NRdP(═O)2NRbRc, NRbC(═O)Ra, or NRbP(═O)2Re, wherein each occurance of Ra is independently hydrogen, alkyl, cycloalkyl, alkenyl, cycloalkenyl, alkynyl, heterocycle, or aryl; each occurance of Rb, Rc and Rd is independently hydrogen, alkyl, cycloalkyl, heterocycle, aryl, or said Rb and Rc together with the N to which they are bonded optionally form a heterocycle; and each occurance of Re is independently alkyl, cycloalkyl, alkenyl, cycloalkenyl, alkynyl, heterocycle, or aryl. The exemplary substitutents can themselves be optionally substituted. Exemplary substituents also include spiro-attached or fused cyclic substituents, especially spiro-attached cycloalkyl, spiro-attached cycloalkenyl, spiro-attached heterocycle (excluding heteroaryl), fused cycloalkyl, fused cycloalkenyl, fused heterocycle, or fused aryl, where the aforementioned cycloalkyl, cycloalkenyl, heterocycle and aryl substituents can themselves be optionally substituted.
The term “aryl” refers to cyclic, aromatic hydrocarbon groups that have 1 to 5 aromatic rings, especially monocyclic or bicyclic groups such as phenyl, biphenyl or naphthyl. Where containing two or more aromatic rings (bicyclic, etc.), the aromatic rings of the aryl group may be joined at a single point (e.g., biphenyl), or fused (e.g., naphthyl, phenanthrenyl and the like). “Substituted aryl” refers to an aryl group substituted by one or more substituents, preferably 1 to 3 substituents, at any available point of attachment. Exemplary substituents include but are not limited to one or more of the following groups: hydrogen, halogen (e.g., a single halogen substituent or multiple halo substitutents forming, in the latter case, groups such as CF3 or an alkyl group bearing Cl3), cyano, nitro, oxo (i.e., ═O), CF3, OCF3, cycloalkyl, alkenyl, cycloalkenyl, alkynyl, heterocycle, aryl, ORa, SRa, S(═O)Re, S(═O)2Re, P(═O)2Re, S(═O)2ORe, P(═O)2ORe, NRbRc, NRbS(═O)2Re, NRbP(═O)2Re, S(═O)2NRbRc, P(═O)2NRbRc, C(═O)ORd, C(═O)Ra, C(═O)NRbRc, OC(═O)Ra, OC(═O)NRbRc, NRbC(═O)ORe, NRdC(═O)NRbRc, NRdS(═O)2NRbRc, NRdP(═O)2NRbRc, NRbC(═O)Ra, or NRbP(═O)2Re, wherein each occurance of Ra is independently hydrogen, alkyl, cycloalkyl, alkenyl, cycloalkenyl, alkynyl, heterocycle, or aryl; each occurance of Rb, Rc and Rd is independently hydrogen, alkyl, cycloalkyl, heterocycle, aryl, or said Rb and Rc together with the N to which they are bonded optionally form a heterocycle; and each occurance of Re is independently alkyl, cycloalkyl, alkenyl, cycloalkenyl, alkynyl, heterocycle, or aryl. The exemplary substitutents can themselves be optionally substituted. Exemplary substituents also include fused cyclic groups, especially fused cycloalkyl, fused cycloalkenyl, fused heterocycle, or fused aryl, where the aforementioned cycloalkyl, cycloalkenyl, heterocycle and aryl substituents can themselves be optionally substituted.
The terms “heterocycle” and “heterocyclic” refer to fully saturated, or partially or fully unsaturated, including aromatic (i.e., “heteroaryl”) cyclic groups (for example, 4 to 7 membered monocyclic, 7 to 11 membered bicyclic, or 8 to 16 membered tricyclic ring systems) which have at least one heteroatom in at least one carbon atom-containing ring. Each ring of the heterocyclic group containing a heteroatom may have 1, 2, 3, or 4 heteroatoms selected from nitrogen atoms, oxygen atoms and/or sulfur atoms, where the nitrogen and sulfur heteroatoms may optionally be oxidized and the nitrogen heteroatoms may optionally be quaternized. (The term “heteroarylium” refers to a heteroaryl group bearing a quaternary nitrogen atom and thus a positive charge.) The heterocyclic group may be attached to the remainder of the molecule at any heteroatom or carbon atom of the ring or ring system. Exemplary monocyclic heterocyclic groups include azetidinyl, pyrrolidinyl, pyrrolyl, pyrazolyl, oxetanyl, pyrazolinyl, imidazolyl, imidazolinyl, imidazolidinyl, oxazolyl, oxazolidinyl, isoxazolinyl, isoxazolyl, thiazolyl, thiadiazolyl, thiazolidinyl, isothiazolyl, isothiazolidinyl, furyl, tetrahydrofuryl, thienyl, oxadiazolyl, piperidinyl, piperazinyl, 2-oxopiperazinyl, 2-oxopiperidinyl, 2-oxopyrrolodinyl, 2-oxoazepinyl, azepinyl, hexahydrodiazepinyl, 4-piperidonyl, pyridyl, pyrazinyl, pyrimidinyl, pyridazinyl, triazinyl, triazolyl, tetrazolyl, tetrahydropyranyl, morpholinyl, thiamorpholinyl, thiamorpholinyl sulfoxide, thiamorpholinyl sulfone, 1,3-dioxolane and tetrahydro-1,1-dioxothienyl, and the like. Exemplary bicyclic heterocyclic groups include indolyl, isoindolyl, benzothiazolyl, benzoxazolyl, benzoxadiazolyl, benzothienyl, benzo[d][1,3]dioxolyl, 2,3-dihydrobenzo[b][1,4]dioxinyl, quinuclidinyl, quinolinyl, tetrahydroisoquinolinyl, isoquinolinyl, benzimidazolyl, benzopyranyl, indolizinyl, benzofuryl, benzofurazanyl, chromonyl, coumarinyl, benzopyranyl, cinnolinyl, quinoxalinyl, indazolyl, pyrrolopyridyl, furopyridinyl (such as furo[2,3-c]pyridinyl, furo[3,2-b]pyridinyl] or furo[2,3-b]pyridinyl), dihydroisoindolyl, dihydroquinazolinyl (such as 3,4-dihydro-4-oxo-quinazolinyl), triazinylazepinyl, tetrahydroquinolinyl and the like. Exemplary tricyclic heterocyclic groups include carbazolyl, benzidolyl, phenanthrolinyl, acridinyl, phenanthridinyl, xanthenyl and the like.
“Substituted heterocycle” and “substituted heterocyclic” (such as “substituted heteroaryl”) refer to heterocycle or heterocyclic groups substituted with one or more substituents, preferably 1 to 4 substituents, at any available point of attachment. Exemplary substituents include but are not limited to one or more of the following groups: hydrogen, halogen (e.g., a single halogen substituent or multiple halo substitutents forming, in the latter case, groups such as CF3 or an alkyl group bearing Cl3), cyano, nitro, oxo (i.e., ═O), CF3, OCF3, cycloalkyl, alkenyl, cycloalkenyl, alkynyl, heterocycle, aryl, ORa, SRa, S(═O)Re, S(═O)2Re, P(═O)2Re, S(═O)2ORe, P(═O)2ORe, NRbRc, NRbS(═O)2Re, NRbP(═O)2Re, S(═O)2NRbRc, P(═O)2NRbRc, C(═O)ORd, C(═O)Ra, C(═O)NRbRc, OC(═O)Ra, OC(═O)NRbRc, NRbC(═O)ORe, NRdC(═O)NRbRc, NRdS(═O)2NRbRc, NRdP(═O)2NRbRc, NRbC(═O)Ra, or NRbP(═O)2Re, wherein each occurance of Ra is independently hydrogen, alkyl, cycloalkyl, alkenyl, cycloalkenyl, alkynyl, heterocycle, or aryl; each occurance of Rb, Rc and Rd is independently hydrogen, alkyl, cycloalkyl, heterocycle, aryl, or said Rb and Rc together with the N to which they are bonded optionally form a heterocycle; and each occurance of Re is independently alkyl, cycloalkyl, alkenyl, cycloalkenyl, alkynyl, heterocycle, or aryl. The exemplary substitutents can themselves be optionally substituted. Exemplary substituents also include spiro-attached or fused cyclic substituents at any available point or points of attachment, especially spiro-attached cycloalkyl, spiro-attached cycloalkenyl, spiro-attached heterocycle (excluding heteroaryl), fused cycloalkyl, fused cycloalkenyl, fused heterocycle, or fused aryl, where the aforementioned cycloalkyl, cycloalkenyl, heterocycle and aryl substituents can themselves be optionally substituted.
The term “quaternary nitrogen” refers to a tetravalent positively charged nitrogen atom including, for example, the positively charged nitrogen in a tetraalkylammonium group (e.g., tetramethylammonium, N-methylpyridinium), the positively charged nitrogen in protonated ammonium species (e.g., trimethyl-hydroammonium, N-hydropyridinium), the positively charged nitrogen in amine N-oxides (e.g., N-methyl-morpholine-N-oxide, pyridine-N-oxide), and the positively charged nitrogen in an N-amino-ammonium group (e.g., N-aminopyridinium).
The terms “halogen” or “halo” refer to chlorine, bromine, fluorine or iodine.
The term “carbocyclic” refers to aromatic or non-aromatic 3 to 7 membered monocyclic and 7 to 11 membered bicyclic groups, in which all atoms of the ring or rings are carbon atoms. “Substituted carbocyclic” refers to a carbocyclic group substituted with one or more substituents, preferably 1 to 4 substituents, at any available point of attachment. Exemplary substituents include but are not limited to one or more of the following groups: hydrogen, halogen (e.g., a single halogen substituent or multiple halo substitutents forming, in the latter case, groups such as CF3 or an alkyl group bearing Cl3), cyano, nitro, oxo (i.e., ═O), CF3, OCF3, cycloalkyl, alkenyl, cycloalkenyl, alkynyl, heterocycle, aryl, ORa, SRa, S(═O)Re, S(═O)2Re, P(═O)2Re, S(═O)2ORe, P(═O)2ORe, NRbRc, NRbS(═O)2Re, NRbP(═O)2Re, S(═O)2NRbRc, P(═O)2NRbRc, C(═O)ORd, C(═O)Ra, C(═O)NRbRc, OC(═O)Ra, OC(═O)NRbRc, NRbC(═O)ORe, NRdC(═O)NRbRc, NRdS(═O)2NRbRc, NRdP(═O)2NRbRc, NRbC(═O)Ra, or NRbP(═O)2Re, wherein each occurance of Ra is independently hydrogen, alkyl, cycloalkyl, alkenyl, cycloalkenyl, alkynyl, heterocycle, or aryl; each occurance of Rb, Rc and Rd is independently hydrogen, alkyl, cycloalkyl, heterocycle, aryl, or said Rb and Rc together with the N to which they are bonded optionally form a heterocycle; and each occurance of Re is independently alkyl, cycloalkyl, alkenyl, cycloalkenyl, alkynyl, heterocycle, or aryl. The exemplary substitutents can themselves be optionally substituted. Exemplary substituents also include spiro-attached or fused cyclic substituents, especially spiro-attached cycloalkyl, spiro-attached cycloalkenyl, spiro-attached heterocycle (excluding heteroaryl), fused cycloalkyl, fused cycloalkenyl, fused heterocycle, or fused aryl, where the aforementioned cycloalkyl, cycloalkenyl, heterocycle and aryl substitutents can themselves be optionally substituted.
When a functional group is termed “protected”, this means that the group is in modified form to mitigate, especially preclude, undesired side reactions at the protected site. Suitable protecting groups for the methods and compounds described herein include, without limitation, those described in standard textbooks, such as Greene, T. W. et al., Protective Groups in Organic Synthesis, 3rd edition, Wiley, N.Y. (1999).
Unless otherwise indicated, any heteroatom with unsatisfied valences is assumed to have hydrogen atoms sufficient to satisfy the valences.
The compounds of formulae I through VI form salts which are also within the scope of this invention. Reference to a compound of formulae I through VI herein is understood to include reference to salts thereof, unless otherwise indicated. The term “salt(s)”, as employed herein, denotes acidic and/or basic salts formed with inorganic and/or organic acids and bases. In addition, when a compound of formulae I through VI contains both a basic moiety, such as but not limited to a pyridine or imidazole, and an acidic moiety such as but not limited to a carboxylic acid, zwitterions (“inner salts”) may be formed and are included within the term “salt(s)” as used herein. Pharmaceutically acceptable (i.e., non-toxic, physiologically acceptable) salts are preferred, although other salts are also useful, e.g., in isolation or purification steps which may be employed during preparation. Salts of the compounds of the formulae I through VI may be formed, for example, by reacting a compound I with an amount of acid or base, such as an equivalent amount, in a medium such as one in which the salt precipitates or in an aqueous medium followed by lyophilization.
The compounds of formulae I through VI which contain a basic moiety, such as but not limited to an amine or a pyridine or imidazole ring, may form salts with a variety of organic and inorganic acids. Exemplary acid addition salts include acetates (such as those formed with acetic acid or trihaloacetic acid, for example, trifluoroacetic acid), adipates, alginates, ascorbates, aspartates, benzoates, benzenesulfonates, bisulfates, borates, butyrates, citrates, camphorates, camphorsulfonates, cyclopentanepropionates, digluconates, dodecylsulfates, ethanesulfonates, fumarates, glucoheptanoates, glycerophosphates, hemisulfates, heptanoates, hexanoates, hydrochlorides, hydrobromides, hydroiodides, hydroxyethanesulfonates (e.g., 2-hydroxyethanesulfonates), lactates, maleates, methanesulfonates, naphthalenesulfonates (e.g., 2-naphthalenesulfonates), nicotinates, nitrates, oxalates, pectinates, persulfates, phenylpropionates (e.g., 3-phenylpropionates), phosphates, picrates, pivalates, propionates, salicylates, succinates, sulfates (such as those formed with sulfuric acid), sulfonates, tartrates, thiocyanates, toluenesulfonates such as tosylates, undecanoates, and the like.
The compounds of formulae I through VI which contain an acidic moiety, such but not limited to a carboxylic acid, may form salts with a variety of organic and inorganic bases. Exemplary basic salts include ammonium salts, alkali metal salts such as sodium, lithium and potassium salts, alkaline earth metal salts such as calcium and magnesium salts, salts with organic bases (for example, organic amines) such as benzathines, dicyclohexylamines, hydrabamines (formed with N,N-bis(dehydroabietyl)ethylenediamine), N-methyl-D-glucamines, N-methyl-D-glycamides, t-butyl amines, and salts with amino acids such as arginine, lysine and the like. Basic nitrogen-containing groups may be quaternized with agents such as lower alkyl halides (e.g., methyl, ethyl, propyl, and butyl chlorides, bromides and iodides), dialkyl sulfates (e.g., dimethyl, diethyl, dibutyl, and diamyl sulfates), long chain halides (e.g., decyl, lauryl, myristyl and stearyl chlorides, bromides and iodides), aralkyl halides (e.g., benzyl and phenethyl bromides), and others.
Prodrugs and solvates of the compounds of the invention are also contemplated herein. The term “prodrug” as employed herein denotes a compound that, upon administration to a subject, undergoes chemical conversion by metabolic or chemical processes to yield a compound of the formulae I through VI, or a salt and/or solvate thereof. Solvates of the compounds of formulae I through VI include, for example, hydrates.
Compounds of the formulae I through VI, and salts thereof, may exist in their tautomeric form (for example, as an amide or imino ether). All such tautomeric forms are contemplated herein as part of the present invention.
All stereoisomers of the present compounds (for example, those which may exist due to asymmetric carbons on various substituents), including enantiomeric forms and diastereomeric forms, are contemplated within the scope of this invention. Individual stereoisomers of the compounds of the invention may, for example, be substantially free of other isomers (e.g., as a pure or substantially pure optical isomer having a specified activity), or may be admixed, for example, as racemates or with all other, or other selected, stereoisomers. The chiral centers of the present invention may have the S or R configuration as defined by the International Union of Pure and Applied Chemistry (IUPAC) 1974 Recommendations. The racemic forms can be resolved by physical methods, such as, for example, fractional crystallization, separation or crystallization of diastereomeric derivatives or separation by chiral column chromatography. The individual optical isomers can be obtained from the racemates by any suitable method, including without limitation, conventional methods, such as, for example, salt formation with an optically active acid followed by crystallization.
Compounds of the formulae I through VI are, subsequent to their preparation, preferably isolated and purified to obtain a composition containing an amount by weight equal to or greater than 99% formulae I through VI compound (“substantially pure” compound I), which is then used or formulated as described herein. Such “substantially pure” compounds of the formulae I through VI are also contemplated herein as part of the present invention.
All configurational isomers of the compounds of the present invention are contemplated, either in admixture or in pure or substantially pure form. The definition of compounds of the present invention embraces both cis (Z) and trans (E) alkene isomers, as well as cis and trans isomers of cyclic hydrocarbon or heterocyclic rings.
Throughout the specifications, groups and substituents thereof may be chosen to provide stable moieties and compounds.
ACN acetonitrile
ArCHO aryl-aldehyde, such as benzaldehyde
Axin2 Axin-related protein
BMP4 bone morphogenetic protein 4
Boc t-butoxycarbonyl
CCD charge-coupled device
CH2Cl2 dichloromethane
CH2O formaldehyde
CHCl3 chloroform
c-myc v-myc myelocytomatosis viral oncogene homolog (avian)
CO carbon monoxide
Conc. concentrated
CSF2 colony stimulating factor 2 (granulocyte-macrophage)
CuI copper iodide
cyclin D1 a member of the cyclin family
DHRS9/DHRL dehydrogenase/reductase (SDR family) member 9
EDCl 1-ethyl-3(3-dimethyl aminopropyl)carbodiimide hydrochloride
Et3N or NEt3 triethyl amine
EtI ethyl iodide
EtOAc ethyl acetate
EtOH ethyl alcohol
FBS fetal bovine serum
GPR49 leucine-rich repeat-containing G protein-coupled receptor 5 (a/k/a LGR5)
HCl hydrochloric acid
HCO2Na sodium formate
HF hydrogen floride
HOBT 1-hydroxybenzotriazole
HPLC High performance liquid chromatography
ID2 inhibitor of DNA binding 2, dominant negative helix-loop-helix protein
K2CO3 potassium carbonate
KLF4 Kruppel-like factor 4 (gut)
MDR-1 ATP-binding cassette, sub-family B (MDR/TAP), member 1
Me2CO propan-2-one
MgSO4 magnesium sulfate
MSX1 msh homeo box homolog 1 (Drosophila)
NaBH3CN sodium cyanotrihydroborate
NaBH4 sodium borohydride
NaHCO3 Sodium bicarbonate
NH4OH ammonim hydroxide
PCl5 phosphorus pentachloride
Pd(PPh3)4 tetrakis(triphenylphosphine)palladium(0)
Pd—C/H2 palladium-carbon/hydrogen
PdCl2(PPh3)2 dichlorobis(triphenylphosphine)palladium(II)
pGL3 Luciferase Reporter Vector
POCl3 phosphorus oxytrichloride
ROR1 receptor tyrosine kinase-like orphan receptor 1
RT or rt room temperature
SnCl2 tin chloride
SV-40 Simian vacuolating virus 40
SV40-Luc a luciferase reporter gene driven by SV40 promoter
SV40-R-Luc a renilla luciferase reporter gene driven by SV40 promoter
SW480 a human colon cancer cell line
TEA triethyl amine
TFA trifluoroacetic acid
TIMP2 tissue inhibitor of metalloproteinase 2
TLC thin layer chormoatograph
Zn(CN)2 zinc cyanide
ZnCl2 zinc chloride
The compounds of the present invention can be prepared using the methods described below, together with synthetic methods known one skilled in the art of organic synthesis, or variations thereon. The reactions are performed in solvents appropriate to the reagents and materials employed and are suitable for transformations being effected. The starting materials for the examples contained herein are either commercially available or are readily prepared by standard methods from known materials. For example, the following reactions are illustrations but not limitations of the preparation of some of the starting materials and examples used herein.
Compounds of formula I can be prepared starting from appropriately substituted 2-aminobenzoic acid derivatives as outlined in Scheme 1. The appropriately substituted 2-aminobenzoic acids 1 can react with urea, preferably at elevated temperatures such as 180-220° C. and in the presence of hydrochloric acid (HCl) to give compound 2. Compound 2 can further react with at least one chlorinating agent such as phosphorus oxytrichloride (POCl3) and/or phosphorus pentachloride (PCl5) to provide dichloro derivatives II. As shown in Path A, dichloro derivatives II can react with an amine of formula III in the presence of a base, such as triethyl amine (Et3N), and in an organic solvent such as chloroform (CHCl3) to afford compound IV. Compound IV can further react with an amine of formula HNR6R7 in the presence of a base, such as triethyl amine, to provide compound I.
Alternatively, as shown in Path B, dichloro derivatives II can react with an amine of formula V to afford compound VI, which in turn can react with an acid chloride of formula R14(C═O)Cl in the presense of a base, or an acid of formula R14(C═O)OH in the presence of an amide coupling agent and a base, to give compound IV. Compound IV can further react with an amine of formula HNR6R7 in the presence of a base, such as triethyl amine, to provide compound I.
Dichloro derivatives II can also be prepared starting from aniline 8 as outlined in Scheme 2. Aniline 8 can react with ethyl isothioscyanatoformate to give intermediate 9, which can further react with ethyl iodide (EtI) in the presense of a base, such as potassium carbonate (K2CO3), to provide intermediate 10. Intermediate 10 can undergo cyclization at elevated temperature to afford compound 11, which can be further transformed to compound 2 under acidic condition. Finally, compound 2 can react with at least one chlorinating agent such as POCl3/PCl5 to provide dichloro derivatives II.
According to Scheme 3, the compound of formula I having amino-substituted structures (e.g., compounds 13, 14-1, 14-2, and 15) can be prepared starting from their respective nitro derivatives 12. The nitro derivatives 12 can be reduced to amino-substituted compounds 13 using palladium-carbon/hydrogen (Pd—C/H2) or tin chloride (SnCl2). The resulting compounds 13 can used to prepare N,N-dialkyl derivatives 14-1 or 14-2 via reductive amination. Compounds 13 can also react with acidchlorides to obtain compound 15.
The compound of formula I having a piperidine moiety (e.g., compounds 18, 19, 20 and 21) can be prepared staring from appropriately substituted 2,4-dichloro derivatives 11 and t-Boc-protected compound 16 according to Scheme 4 and Scheme 5. Intermediate 17 can be aminated using primary or secondary amines. The Boc-group can be removed by using an acid, such as trifluoroacetic acid (TFA), and various R13 groups can be introduced. As a non-limiting example, various alkyl and benzyl substituents can be introduced at the nitrogen of the piperidine moiety, by a reductive amination process.
To a stirred solution of 122 mg (1 mmol) 4-aminobenzylamine and 202 mg (2 mmol) triethylamine in 5 mL CHCl3 at rt was added 1 mmol of the appropriately substituted 2,4-dichloroquinazoline was added and stirred for a minimum of 3 hours. After thin layer chormoatograph (TLC) showed the complete disappearance of the 2,4-dichloroquinazoline, 1 mmol of aroylchloride in 1 mL CHCl3 was added slowly. The reaction mixture was allowed to stir overnight. At the end the reaction mixture was quenched with 50 mL of CHCl3 and 15 mL water. The reaction mixture was washed well with water and the chloroform layer was dried over magnesium sulfate (MgSO4). After removal of MgSO4 by filtration and evaporation of solvents the crude product was purified by column chromatography with hexane/CH2Cl2/triethyl amine (TEA) to give the 2-chloroquinazolines in yields between 50-95%.
The appropriately substituted 2-chloro quinazoline derivatives (1 mmol) obtained by the Procedure A, step 1 was taken up either in a sealed tube or in round bottom flask and was suspended in 5 mL THF or dioxane. (If the reactant amine was mono methylamine or dimethylamine sealed tube was used and for other amines round bottom flask can be used.) The appropriate amine was added and the mixture was heated over 16 h to 100° C. or alternatively heated for 40 min to 120° C. using microwave. After reaction was completed the solvents were removed in vacuo and the crude compound purified by silica-gel column chromatography by using CH2Cl2/MeOH/NH3-mixtures as eluent to give the diamino quinazolines in yields between 65-95%.
5-fluoro-2-methyl-N-[4-({[2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]benzamide was prepared starting from 2,4-dichloroquinazoline, 4-aminobenzylamine and 2-methyl-4-fluorobenzoyl chloride by following the procedure A (step 1). The intermediate product from the step 1 was aminated using monomethylamine to yield the final product. Starting from 2,4-dichloroquinazoline (0.140 g, 0.7 mmol), 25 mg (Yield, 18%) of the final product was isolated. MS (ESI) m/z 416.2.
2-(Benzyloxy)-N-[4-({[2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]acetamide was prepared starting from 2,4-dichloroquinazoline, 4-aminobenzylamine and benzyloxy-acetyl chloride by following the procedure A (step 1). The intermediate product from the step 1 was aminated using monomethylamine to yield the final product. Starting from 2,4-dichloroquinazoline, (0.20 g, 1.0 mmol), 50 mg (Yield, 25%) of the final product was isolated. MS (ESI) m/z 428.2.
6-chloro-N-[4-({[2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]nicotinamide was prepared starting from 2,4-dichloroquinazoline, 4-aminobenzylamine and 6-chloronicotinoyl chloride by following the procedure A (step 1). The intermediate product from the step 1 was aminated using monomethylamine to yield the final product. Starting from 2,4-dichloroquinazoline, (0.15 g, 0.75 mmol) 140 mg (Yield, 95%) of the final product was isolated. MS (ESI) m/z 419.1.
2-chloro-N-[4-({[2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]isonicotinamide was prepared starting from 2,4-dichloroquinazoline, 4-aminobenzylamine and 2-chloro-isonicotinoyl chloride by following the procedure A (step 1). The intermediate product from the step 1 was aminated using monomethylamine to yield the final product. Starting from 2,4-dichloroquinazoline, (0.18 g, 0.9 mmol), 40 mg (Yield, 24%) of the final product was isolated. MS (ESI) m/z 419.8.
N-[4-({[2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]quinoline-2-carboxamide was prepared starting from 2,4-dichloroquinazoline, 4-aminobenzylamine and 2-chloro-isoquinoline-3-carbonyl chloride by following the procedure A (step 1). The intermediate product from the step 1 was aminated using monomethylamine to yield the final product. Starting from 2,4-dichloroquinazoline, (0.20 g, 1.0 mmol), 16 mg (18%) of the final product was isolated. MS (ESI) 436.
2-chloro-5-fluoro-N-[4-({[2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]benzamide was prepared starting from 2,4-dichloroquinazoline, 4-aminobenzylamine and 2-chloro-5-fluorobenzoylchloride by following the procedure A (step 1). The intermediate product from the step 1 was aminated using monomethylamine to yield the final product. Starting from 2,4-dichloroquinazoline, (0.20 g, 1.0 mmol), 18 mg (Yield, 9%) of the final product was isolated. MS (ESI) m/z 436.5.
2-chloro-N-[4-({[2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]nicotinamide was prepared starting from 2,4-dichloroquinazoline, 4-aminobenzylamine and 2-chloronicotinoyl chloride by following the procedure A (step 1). The intermediate product from the step 1 was aminated using monomethylamine to yield the final product. Starting from 2,4-dichloroquinazoline, (0.20 g, 1.0 mmol), 21 mg (Yield, 11% of the final product was isolated. MS (ESI) m/z 419.
2,6-dichloro-5-fluoro-N-[4-({[2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]nicotinamide was prepared starting from 2,4-dichloroquinazoline, 4-aminobenzylamine and 2,6-dichloro-5-fluoro-nicotinoylchloride following the procedure A (step 1). The intermediate product from the step 1 was aminated using monomethylamine to yield the final product. Starting from 2,4-dichloroquinazoline, (0.20 g, 1.0 mmol), 150 mg (Yield, 58%) of the final product was isolated. MS (ESI) m/z 471.
6-chloro-N-[4-({[6,7-dimethoxy-2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]nicotinamide was prepared starting from 2,4-dichloro-6,7-dimethoxyquinazoline, 4-aminobenzylamine and 6-chloronicotinoyl chloride by following the procedure A (step 1). The intermediate product from the step 1 was aminated using monomethylamine to yield the final product. Starting from 2,4-dichloro-6,7-dimethoxyquinazoline, (0.20 g, 1.0 mmol), 71 mg (Yield, 36%) of the final product was isolated. MS (ESI) m/z 479.1.
4-chloro-N-[4-({[2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]benzamide was prepared starting from 2,4-dichloroquinazoline, 4-aminobenzylamine and 4-chlorobenzoyl chloride by following the procedure A (step 1). The intermediate product from the step 1 was aminated using monomethylamine to yield the final product. Starting from 2,4-dichloroquinazoline, (0.10 g, 0.5 mmol), 20 mg (Yield, 41%) of the final product was isolated. MS (ESI) m/z 418.1.
3-chloro-N-[4-({[2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]benzamide was prepared starting from 2,4-dichloroquinazoline, 4-aminobenzylamine and 3-chlorobenzoyl chloride by following the procedure A (step 1). The intermediate product from the step 1 was aminated using monomethylamine to yield the final product. Starting from 2,4-dichloroquinazoline, (0.10 g, 0.5 mmol) 30 mg (Yield, 30%) of the final product was isolated. MS (ESI) m/z 418.1.
4-methyl-N-[4-({[2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]benzamide was prepared starting from 2,4-dichloroquinazoline, 4-aminobenzylamine and 4-methyl-benzoylchloride by following the procedure A (step 1). The intermediate product from the step 1 was aminated using monomethylamine to yield the final product. Starting from of 2,4-dichloroquinazoline, (0.50 g, 2.5 mmol) of 200 mg (Yield, 40%) of the final product was isolated. MS (ESI) m/z 398.2.
4-fluoro-N-[4-({[2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]benzamide was prepared starting from 2,4-dichloroquinazoline, 4-aminobenzylamine and 4-fluorobenzoylchloride by following the procedure A (step 1). The intermediate product from the step 1 was aminated using monomethylamine to yield the final product. Starting from 2,4-dichloroquinazoline, (0.38 g, 1.9 mmol), 010 mg (Yield, 55%) of the final product was isolated. MS (ESI) m/z 402.1.
4-chloro-2-fluoro-N-[4-({[2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]benzamide was prepared starting from 2,4-dichloroquinazoline, 4-aminobenzylamine, and 2-fluoro-4-chlorobenzoylchloride by following the procedure A (step 1). The intermediate product from the step 1 was aminated using monomethylamine to yield the final product. Starting from 2,4-dichloroquinazoline, (0.20 g, 1.0 mmol), 70 mg (Yield, 63%) of the final product was isolated. MS (ESI) m/z 436.1.
N-[4-({[2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]biphenyl-4-carboxamide was prepared starting from 2,4-dichloroquinazoline, 4-aminobenzylamine and biphenyl-4-carbonyl chloride by following the procedure A (step 1). The intermediate product from the step 1 was aminated using monomethylamine to yield the final product. Starting from 2,4-dichloroquinazoline, (0.17 g, 0.85 mmol) 50 mg (Yield, 49%) of the final product was isolated. MS (ESI) m/z 460.2.
2,4-dichloro-N-[4-({[2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]benzamide was prepared starting from 2,4-dichloroquinazoline, 4-aminobenzylamine and 2,4-dichlorobenzoyl chloride by following the procedure A (step 1). The intermediate product from the step 1 was aminated using monomethylamine to yield the final product. Starting from 2,4-dichloroquinazoline, (0.16 g, 0.8 mmol) 40 mg (Yield, 57%) of the final product was isolated. MS (ESI) m/z 452.2.
4-fluoro-3-methyl-N-[4-({[2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]benzamide was prepared starting from 2,4-dichloroquinazoline, 4-aminobenzylamine and 3methyl-4-chloro benzoylchloride by following the procedure A (step 1). The intermediate product from the (step 1) was aminated using monomethylamine to yield the final product. Starting from 2,4-dichloroquinazoline, (0.155 g, 0.77 mmol) 50 mg Yield, 41%) of the final product was isolated. MS (ESI) m/z 416.
4-chloro-N-[4-({[6-methyl-2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]benzamide was prepared starting from 6-methyl-2,4-dichloroquinazoline, 4-aminobenzylamine and 4-chloro benzoylchloride by following the procedure A (step 1). The intermediate product from the (step 1) was aminated using monomethylamine to yield the final product. Starting from 6-methyl-2,4-dichloroquinazoline, (0.7 g, 3.5 mmol), 400 mg (Yield, 61%) of the final product was isolated. (ESI) m/z 432.3.
2,4-dichloro-N-[4-({[6-methyl-2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]benzamide was prepared starting from 6-methyl-2,4-dichloroquinazoline, 4-aminobenzylamine and 2,4-dichloro benzoylchloride by following the procedure A (step 1). The intermediate product from the (step 1) was aminated using monomethylamine to yield the final product. Starting from 6-methyl-2,4-dichloroquinazoline, (0.21 g, 1.0 mmol) 40 mg (Yield, 50%) of the final product was isolated. MS (ESI) m/z 466.1.
N-[4-({[2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]-4-(trifluoromethyl)benzamide was prepared starting from 2,4-dichloroquinazoline, 4-aminobenzylamine and 4-trifluoromethyl benzoylchloride by following the procedure A (step 1). The intermediate product from the (step 1) was aminated using monomethylamine to yield the final product. Starting from 2,4-dichloroquinazoline, (0.19 g, 1.0 mmol) 50 mg (Yield, 43%) of the final product was isolated. MS (ESI) m/z 452.2.
4-cyano-N-[4-({[2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]benzamide was prepared starting from 2,4-dichloroquinazoline, 4-aminobenzylamine and 4-cyano-benzoylchloride by following the procedure A (step 1). The intermediate product from the (step 1) was aminated using monomethylamine to yield the final product. Starting from 2,4-dichloroquinazoline, (0.19 g, 1.0 mmol), 30 mg (Yield, 16%) of the final product was isolated. MS (ESI) m/z 409.
N-[4-({[2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]-4-(trifluoromethoxy)benzamide was prepared starting from 2,4-dichloroquinazoline, 4-aminobenzylamine and 4-trifluoromethoxy-benzoylchloride by following the procedure A (step 1). The intermediate product from the (step 1) was aminated using monomethylamine to yield the final product. Starting from (0.19 g, 1.0 mmol) of 2,4-dichloroquinazoline, (0.19 g, 1.0 mmol), 30 mg (Yield, 31%) of the final product was isolated. MS (ESI) m/z 468.
6-chloro-N-[4-({[6,8-dimethyl-2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]nicotinamide was prepared starting from 6,8-dimethyl-2,4-dichloroquinazoline, 4-aminobenzylamine and 6-nicotinoyl chloride by following the procedure A (step 1). The intermediate product from the (step 1) was aminated using monomethylamine to yield the final product. Starting from 6,8-dimethyl-2,4-dichloroquinazoline, (0.226, 1.0 mmol), 100 mg Yield, 17%) of the final product was isolated. MS (ESI) m/z 447.
4-fluoro-N-[4-({[6-methyl-2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]benzamide was prepared starting from 6-methyl-2,4-dichloroquinazoline, 4-aminobenzylamine and 4-fluorobenzoylchloride by following the procedure A (step 1). The intermediate product from the (step 1) was aminated using monomethylamine to yield the final product. Starting from (0.41 g, 2.0 mmol) of 6-methyl-2,4-dichloroquinazoline, 200 mg Yield, 58%) of the final product was isolated. MS (ESI) m/z 416.2.
4-cyano-N-[4-({[6-methyl-2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]benzamide was prepared starting from 6-methyl-2,4-dichloroquinazoline, 4-aminobenzylamine and 4-cyanobenzoylchloride by following the procedure A (step 1). The intermediate product from the (step 1) was aminated using monomethylamine to yield the final product. Starting from 6-methyl-2,4-dichloroquinazoline, (0.41 g, 2.0 mmol) 250 mg (Yield, 59%) of the final product was isolated. MS (ESI) m/z 423.2.
4-chloro-2-fluoro-N-[4-({[6-methyl-2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]benzamide was prepared starting from 6-methyl-2,4-dichloroquinazoline, 4-aminobenzylamine and 2-fluoro-4-chlorobenzoylchloride by following the procedure A (step 1). The intermediate product from the (step 1) was aminated using monomethylamine to yield the final product. Starting from 6-methyl-2,4-dichloroquinazoline, (0.41 g, 2.0 mmol), 100 mg (Yield, 24%) of the final product was isolated. MS (ESI) m/z 456.
N-[4-({[6-methyl-2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]-4-(trifluoromethyl)benzamide was prepared starting from 6-methyl-2,4-dichloroquinazoline, 4-aminobenzylamine and 4-trifluoromethyl-benzoylchloride by following the procedure A (step 1). The intermediate product from the (step 1) was aminated using monomethylamine to yield the final product. Starting from 6-methyl-2,4-dichloroquinazoline, (0.19 g, 0.90 mmol) 28 mg (Yield, 28%) of the final product was isolated. MS (ESI) m/z 466.1.
2-chloro-4-fluoro-N-[4-({[6-methyl-2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]benzamide was prepared starting from 6-methyl-2,4-dichloroquinazoline, 4-aminobenzylamine and 2-chloro-4-fluorobenzoylchloride by following the procedure A (step 1). The intermediate product from the (step 1) was aminated using monomethylamine to yield the final product. Starting from 6-methyl-2,4-dichloroquinazoline, (0.41 g, 2.0 mmol), 150 mg (Yield, 44%) of the final product was isolated. MS (ESI) m/z 450.1.
4-chloro-N-[4-({[6,8-dimethyl-2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]benzamide was prepared starting from 6,8-dimethyl-2,4-dichloroquinazoline, 4-aminobenzylamine and 4-chlorobenzoylchloride by following the procedure A (step 1). The intermediate product from the (step 1) was aminated using monomethylamine to yield the final product. Starting from 6,8-dimethyl-2,4-dichloroquinazoline, (0.7 g, 3.0 mmol) 400 mg (Yield, 63%) of the final product was isolated. MS (ESI) m/z 446.2.
N-[4-({[6,8-dimethyl-2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]-4-fluorobenzamide was prepared starting from 6,8-dimethyl-2,4-dichloroquinazoline, 4-aminobenzylamine and 4-fluorobenzoylchloride by following the procedure A (step 1). The intermediate product from the (step 1) was aminated using monomethylamine to yield the final product. Starting from 6,8-dimethyl-2,4-dichloroquinazoline, (0.25 g, 1.0 mmol), 30 mg (Yield, 29%) of the final product was isolated. MS (ESI) m/z 430.1.
6-chloro-N-[4-({[6-methoxy-2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]nicotinamide was prepared starting from 6-methoxy-2,4-dichloroquinazoline, 4-aminobenzylamine and 6-chloronicotinoyl chloride by following the procedure A (step 1). The intermediate product from the (step 1) was aminated using monomethylamine to yield the final product. Starting from 6-methoxy-2,4-dichloroquinazoline, (0.20 g, 0.88 mmol), 30 mg (Yield, 10%) of the final product was isolated. MS (ESI) m/z 449.2.
3,4-difluoro-N-[4-({[6-methyl-2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]benzamide was prepared starting from 6-methyl-2,4-dichloroquinazoline, 4-aminobenzylamine and 3,4-difluorobenzoylchloride by following the procedure A (step 1). The intermediate product from the (step 1) was aminated using monomethylamine to yield the final product. Starting from 6-methyl-2,4-dichloroquinazoline, (0.25 g, 1.0 mmol), 40 mg (Yield, 34%) of the final product was isolated. MS (ESI) m/z 434.2.
4-chloro-N-[4-({[6-methoxy-2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]benzamide was prepared starting from 6-methoxy-2,4-dichloroquinazoline, 4-aminobenzylamine and 4-chlorobenzoylchloride by following the procedure A (step 1). The intermediate product from the (step 1) was aminated using monomethylamine to yield the final product. Starting from 6-methoxy-2,4-dichloroquinazoline, (0.24 g, 1.0 mmol) 40 mg (Yield, 35%) of the final product was isolated. MS (ESI) m/z 448.1.
2,4-difluoro-N-[4-({[6-methyl-2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]benzamide was prepared starting from 6-methyl-2,4-dichloroquinazoline, 4-aminobenzylamine and 2,4-difluorobenzoylchloride by following the procedure A (step 1). The intermediate product from the (step 1) was aminated using monomethylamine to yield the final product. Starting from 6-dimethyl-2,4-dichloroquinazoline, (0.25 g, 1.0 mmol), 25 mg (Yield, 21%) of the final product was isolated; MS (ESI) m/z 434.3.
4-chloro-N-[4-({[6,8-dimethyl-2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]-2-fluorobenzamide was prepared starting from 6,8-dimethyl-2,4-dichloroquinazoline, 4-aminobenzylamine and 2-fluoro-4-chlorobenzoylchloride by following the procedure A (step 1). The intermediate product from the (step 1) was aminated using monomethylamine to yield the final product. Starting from 6,8-dimethyl-2,4-dichloroquinazoline, (0.24 g, 1.0 mmol) 40 mg (Yield, 37%) of the final product was isolated. MS (ESI) m/z 464.1.
N-[4-({[2-(dimethylamino)-6-methylquinazolin-4-yl]amino}methyl)phenyl]-3,4-difluorobenzamide was prepared starting from 6-methyl-2,4-dichloroquinazoline, 4-aminobenzylamine and 3,4-difluorobenzoylchloride by following the procedure A (step 1). The intermediate product from the (step 1) was aminated using dimethylamine to yield the final product. Starting from 6-methyl-2,4-dichloroquinazoline, (0.24 g, 1.0 mmol), 40 mg (Yield, 31%) of the final product was isolated. MS (ESI) m/z 448.4.
3,4-dichloro-N-[4-({[6-methyl-2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]benzamide was prepared starting from 6-methyl-2,4-dichloroquinazoline, 4-aminobenzylamine and 3,4-dichlorobenzoyl chloride by following the procedure A (step 1). The intermediate product from the (step 1) was aminated using monomethylamine to yield the final product. Starting from 6-methyl-2,4-dichloroquinazoline, (0.24 g, 1.0 mmol), 40 mg (Yield, 36%) of the final product was isolated. MS (ESI) m/z 466.3.
N-[4-({[2-(dimethylamino)-6-methylquinazolin-4-yl]amino}methyl)phenyl]-4-fluorobenzamide was prepared starting from 6-methyl-2,4-dichloroquinazoline, 4-aminobenzylamine and 4-fluorobenzoylchloride by following the procedure A (step 1). The intermediate product from the (step 1) was aminated using dimethylamine to yield the final product. Starting from (0.24 g, 1.0 mmol) of 6-dimethyl-2,4-dichloroquinazoline, (0.24 g, 1.0 mmol), 40 mg (Yield, 28%) of the final product was isolated. MS (ESI) m/z 430.3.
A mixture of N-(4-{[(2-chloro-6-methylquinazolin-4-yl)lamino]methyl}phenyl-4-fluorobenzamide) (50 mg, 0.119 mmol), dioxane (3 mL), triethylamine (0.5 mL), dimethylamine (45 mg, 1.0 mmol), was heated to 90° C. during 24 hours. The reaction mixture was cooled at room temperature. The solvent was eliminated and the residue was chromatographed by HPLC to give 22 mg (49% yield) mg of the product.
N-[4-({[2-(dimethylamino)-6-methylquinazolin-4-yl]amino}methyl)phenyl]-4-fluorobenzamide was prepared starting from 6-methyl-2,4-dichloroquinazoline, 4-aminobenzylamine and 4-fluorobenzoylchloride by following the procedure A (step 1). The intermediate product from the (step 1) was aminated using dimethylamine to yield the final product. Starting from (0.24 g, 1.0 mmol) of 6-dimethyl-2,4-dichloroquinazoline, 270 mg (Yield, 63%) of the final product was isolated. MS (ESI) m/z 430.3.
3,5-difluoro-N-[4-({[6-methyl-2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]benzamide was prepared starting from 6-methyl-2,4-dichloroquinazoline, 4-aminobenzylamine and 3,5-difluorobenzoyl chloride by following the procedure A (step 1). The intermediate product from the (step 1) was aminated using monomethylamine to yield the final product. Starting from (0.24 g, 1.0 mmol) of 6-methyl-2,4-dichloroquinazoline, 30 mg (Yield, 28%) of the final product was isolated. MS (ESI) m/z 434.4.
4-fluoro-N-(4-{[(6-methyl-2-piperidin-1-ylquinazolin-4-yl)amino]methyl}phenyl)benzamide was prepared starting from 6-methyl-2,4-dichloroquinazoline, 4-aminobenzylamine and 4-fluorobenzoyl chloride by following the procedure A (step 1). The intermediate product from the (step 1) was aminated using piperidine to yield the final product. Starting from (0.24 g, 1.0 mmol) of 6-methyl-2,4-dichloroquinazoline, 30 mg (Yield, 19%) of the final product was isolated. MS (ESI) m/z 470.3.
A mixture of (N-(4-{[(2-chloro-6-methylquinazolin-4-yl)]amino]methyl}phenyl-4-fluorobenzamide) (50 mg, 0.119 mmol), dioxane (3 mL), triethylamine (0.5 mL), diethylamine (44 mg, 0.59 mmol), was heated to 90° C. during 24 hours. The reaction mixture was cooled at room temperature. The solvent was eliminated and the residue was chromatographed by HPLC to give 3.35 (93% yield) mg of the product.
A mixture of N-(4-((2-chloro-6-methylquinazolin-4-ylamino)methyl)phenyl)-4-fluorobenzamide (50 mg, 0.119 mmol), dioxane (3 mL), triethylamine (0.5 mL), pyrrolidine (42 mg, 0.59 mmol), was heated to 90° C. during 24 hours. The reaction mixture was cooled at room temperature. The solvent was eliminated and the residue was chromatographed by HPLC to give 1.51 (93% yield) mg of the product.
A mixture of N-(4-{[(2-chloro-6-methylquinazolin-4-yl)lamino]methyl}phenyl-4-fluorobenzamide) (50 mg, 0.119 mmol), dioxane (3 mL), Triethylamine (0.5 mL), piperazine (51.2 mg, 0.59 mmol), was heated to 90° C. during 24 hours. The reaction mixture was cooled at room temperature. The solvent was eliminated and the residue was chromatographed by HPLC to give 4.5 mg (79% yield) mg of the product.
A mixture of (N-(4-{[(2-chloro-6-methylquinazolin-4-yl)lamino]methyl}phenyl-4-fluorobenzamide) (50 mg, 0.57 mmol), dioxane (3 mL), Triethylamine (0.5 mL), 1-methylpiperazine (58 mg, 0.58 mmol), was heated to 90° C. during 24 hours. The reaction mixture was cooled at room temperature. The solvent was eliminated and the residue was chromatographed by HPLC to give 6 mg (90% yield) mg of the product.
2-fluoro-N-[4-({[6-methyl-2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]benzamide was prepared starting from 6-methyl-2,4-dichloroquinazoline, 4-aminobenzylamine and 2-fluorobenzoyl chloride by following the procedure A (step 1). The intermediate product from the (step 1) was aminated using monomethylamine to yield the final product. Starting from (0.24 g, 1.0 mmol) of 6-methyl-2,4-dichloroquinazoline, 24 mg (Yield, 17%) of the final product was isolated. MS (ESI) m/z 416.3.
4-fluoro-N-[4-({[6-methyl-2-(4-methylpiperazin-1-yl)quinazolin-4-yl]amino}methyl)phenyl]benzamide was prepared starting from 6-methyl-2,4-dichloroquinazoline, 4-aminobenzylamine and 2-fluorobenzoyl chloride by following the procedure A (step 1). The intermediate product from the (step 1) was aminated using 1-methyl-piperazine to yield the final product. Starting from (0.24 g, 1.0 mmol) of 6-methyl-2,4-dichloroquinazoline, 40 mg (Yield, 15%) of the final product was isolated. MS (ESI) m/z 485.2.
N-[4-({[6,8-dimethyl-2-(4-methylpiperazin-1-yl)quinazolin-4-yl]amino}methyl)phenyl]-4-fluorobenzamide was prepared starting from 6,8-dimethyl-2,4-dichloroquinazoline, 4-aminobenzylamine and 2-fluorobenzoyl chloride by following the procedure A (step 1). The intermediate product from the (step 1) was aminated using methyl-piperazine to yield the final product. Starting from (0.24 g, 1.0 mmol) of 6-methyl-2,4-dichloroquinazoline, 40 mg (Yield, 23%) of the final product was isolated. MS (ESI) m/z 499.1.
N-{4-[({6,8-dimethyl-2-[(3S)-3-methylpiperazin-1-yl]quinazolin-4-yl}amino)methyl]phenyl}-4-fluorobenzamide was prepared starting from 6,8-dimethyl-2,4-dichloroquinazoline, 4-aminobenzylamine and 2-fluorobenzoyl chloride by following the procedure A (step 1). The intermediate product from the (step 1) was aminated using (S)-2-methylpiperazine to yield the final product. Starting from (0.24 g, 1.0 mmol) of 6,8-methyl-2,4-dichloroquinazoline, 28 mg (Yield, 13%) of the final product was isolated. MS (ESI) m/z 416.3.
4-fluoro-N-{4-[({6-methyl-2-[(3S)-3-methylpiperazin-1-yl]quinazolin-4-yl}amino)methyl]phenyl}benzamide was prepared starting from 6-methyl-2,4-dichloroquinazoline, 4-aminobenzylamine and 2-fluorobenzoyl chloride by following the procedure A (step 1). The intermediate product from the (step 1) was aminated using (S)-2-methylpiperazine to yield the final product. Starting from (0.24 g, 1.0 mmol) of 6-methyl-2,4-dichloroquinazoline, 25 mg (Yield, 29%) of the final product was isolated. MS (ESI) m/z 485.2.
N-[4-({[2-(dimethylamino)-6,8-dimethylquinazolin-4-yl]amino}methyl)phenyl]-4-fluorobenzamide was prepared starting from 6,8-dimethyl-2,4-dichloroquinazoline, 4-aminobenzylamine and 2-fluorobenzoyl chloride by following the procedure A (step 1). The intermediate product from the (step 1) was aminated using dimethylamine to yield the final product. Starting from (0.24 g, 1.0 mmol) of 6,8-methyl-2,4-dichloroquinazoline, 60 mg (Yield, 44%) of the final product was isolated. MS (ESI) m/z 444.2.
4-chloro-N-[4-({[2-(dimethylamino)-6-methylquinazolin-4-yl]amino}methyl)phenyl]benzamide was prepared starting from 6-methyl-2,4-dichloroquinazoline, 4-aminobenzylamine and 3,5-difluorobenzoyl chloride by following the procedure A (step 1). The intermediate product from the (step 1) was aminated using dimethylamine to yield the final product. Starting from (0.24 g, 1.0 mmol) of 6-methyl-2,4-dichloroquinazoline, 40 mg (Yield, 35%) of the final product was isolated. MS (ESI) m/z 446.1.
Ethyl 4-[4-({4-[(4-fluorobenzoyl)amino]benzyl}amino)-6-methylquinazolin-2-yl]piperazine-1-carboxylate was prepared starting from 6-methyl-2,4-dichloroquinazoline, 4-aminobenzylamine and 2-fluorobenzoyl chloride by following the procedure A (step 1). The intermediate product from the (step 1) was aminated using piperazine-1-carboxylic ethyl ester to yield the final product. Starting from (0.24 g, 1.0 mmol) of 6-methyl-2,4-dichloroquinazoline, 500 mg (Yield, 65%) of the final product was isolated. MS (ESI) m/z 543.3.
4-fluoro-N-[4-({[6-methyl-2-(4-pyridin-2-ylpiperazin-1-yl)quinazolin-4-yl]amino}methyl)phenyl]benzamide was prepared starting from 6-methyl-2,4-dichloroquinazoline, 4-aminobenzylamine and 2-fluorobenzoyl chloride by following the procedure A (step 1). The intermediate product from the (step 1) was aminated using 1-pyridin-2-piperazine to yield the final product. Starting from (0.24 g, 1.0 mmol) of 6,8-methyl-2,4-dichloroquinazoline, 140 mg (Yield, 44%) of the final product was isolated. MS (ESI) m/z 548.3.
N-(4-{[(2-azepan-1-yl-6-methylquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide was prepared starting from 6-methyl-2,4-dichloroquinazoline, 4-aminobenzylamine and 2-fluorobenzoyl chloride by following the procedure A (step 1). The intermediate product from the (step 1) was aminated using homopiperidine to yield the final product. Starting from (0.24 g, 1.0 mmol) of 6-methyl-2,4-dichloroquinazoline, 60 mg (Yield, 20%) of the final product was isolated. MS (ESI) m/z 484.3.
N-[4-({[2-(4-ethylpiperazin-1-yl)-6-methylquinazolin-4-yl]amino}methyl)phenyl]-4-fluorobenzamide was prepared starting from 6-methyl-2,4-dichloroquinazoline, 4-aminobenzylamine and 2-fluorobenzoyl chloride by following the procedure A (step 1). The intermediate product from the (step 1) was aminated using 4-ethyl piperazine to yield the final product. Starting from (0.24 g, 1.0 mol) of 6-methyl-2,4-dichloroquinazoline, 480 mg (Yield, 74% of the final product was isolated. MS (ESI) m/z 499.3; MS (ESI) m/z 250.1.
4-fluoro-N-{4-[({6-methyl-2-[methyl(pyridin-2-ylmethyl)amino]quinazolin-4-yl}amino)methyl]phenyl}benzamide was prepared starting from 6-methyl-2,4-dichloroquinazoline, 4-aminobenzylamine and 4-fluorobenzoyl-chloride by following the procedure A (step 1). The intermediate product from the (step 1) was aminated using benzyl-methyl-amine to yield the final product. Starting from (0.24 g, 1.0 mmol) of 6-methyl-2,4-dichloroquinazoline, 50 mg (Yield, 18%) of the final product was isolated. MS (ESI) m/z 507.3.
N-{4-[({2-(dimethylamino)-6-[6-(dimethylamino)pyridin-3-yl]quinazolin-4-yl}amino)methyl]phenyl}-4-fluorobenzamide was prepared starting from 6-Iodo-2,4-dichloroquinazoline, 4-aminobenzylamine and 4-fluorobenzoyl-chloride by following the procedure A (step 1). The intermediate product from the (step 1) was aminated using dimethylamine followed by Suzuki coupling with 4-dimethylamine-pyridine boronic acid to yield the final product. Starting from (0.24 g, 1.0 mmol) of 6-iodo-2,4-dichloroquinazoline, 112 mg (Yield, 45%); MS (ESI) m/z 536.1.
N-[4-({[2-(dimethylamino)-6-fluoroquinazolin-4-yl]amino}methyl)phenyl]-4-fluorobenzamide was prepared starting from 6-fluoro-2,4-dichloroquinazoline, 4-aminobenzylamine and 4-fluorobenzoyl-chloride by following the procedure A (step 1). The intermediate product from the (step 1) was aminated using dimethylamine to yield the final product. Starting from (0.24 g, 1.0 mmol) of 6-fluoro-2,4-dichloroquinazoline, 200 mg (Yield, 67%) of the final product was isolated. MS (ESI) m/z 433.
N-[4-({[2-(dimethylamino)-7-isopropylquinazolin-4-yl]amino}methyl)phenyl]-4-fluorobenzamide was prepared starting from 7-iso-propyl-2,4-dichloroquinazoline, 4-aminobenzylamine and 4-fluorobenzoyl chloride by following the procedure A (step 1). The intermediate product from the (step 1) was aminated using dimethylamine to yield the final product. Starting from (0.22 g, 0.92 mmol) of 7-iso-propyl-2,4-dichloroquinazoline, 160 mg Yield, 72%) of the final product was isolated. MS (ES) m/z 458.4.
6-chloro-N-[4-({[2-(dimethylamino)-7-isopropylquinazolin-4-yl]amino}methyl)phenyl]nicotinamide was prepared starting from 7-iso-propyl-2,4-dichloroquinazoline, 4-aminobenzylamine and 6-nicotinoyl chloride by following the procedure A (step 1). The intermediate product from the (step 1) was aminated using dimethylamine to yield the final product. Starting from (0.22 g, 0.92 mmol) of 7-iso-propyl-2,4-dichloroquinazoline, 80 mg (Yield, 40%) of the final product was isolated. MS (ESI) m/z 475.3.
1-Benzyl-N-[4-({[2-(dimethylamino)-7-isopropylquinazolin-4-yl]amino}methyl)phenyl]piperidine-4-carboxamide was prepared starting from 7-iso-propyl-2,4-dichloroquinazoline, 4-aminobenzylamine and 1-benzyl-piperidine-4-carboxylic acid (4-aminomethyl-phenyl)-amide by following the procedure A (step 1). The intermediate product from the (step 1) was aminated using dimethylamine to yield the final product. Starting from (0.22 g, 0.92 mmol) of 7-iso-propyl-2,4-dichloroquinazoline, 35 mg (Yield, 12%) of the final product was isolated. MS (ESI) m/z 537.1.
6-chloro-N-[4-({[2-(dimethylamino)-7-fluoro-8-methylquinazolin-4-yl]amino}methyl)phenyl]nicotinamide was prepared starting from 7-fluoro-8-methyl-propyl-2,4-dichloroquinazoline, 4-aminobenzylamine and 6-nicotinoyl chloride amide by following the procedure A (step 1). The intermediate product from the (step 1) was aminated using dimethylamine to yield the final product. Starting from (0.4 g, 0.88 mmol) of 7-fluoro-8-methyl-propyl-2,4-dichloroquinazoline, 100 mg (Yield, 38%) of the final product was isolated. MS (ESI) m/z 465.3.
6-chloro-N-[4-({[8-methyl-2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]nicotinamide was prepared starting from 8-methyl-2,4-dichloroquinazoline, 4-aminobenzylamine and 6-chloronicotinoylchloride by following the procedure A (step 1). The intermediate product from the step 1 was aminated using monomethylamine to yield the final product. Starting from of 8-methyl-2,4-dichloroquinazoline, (213 mg, 0.1 mmol), 250 mg (Yield, 23%) of the final product was isolated. MS (ESI) m/z 433.2. During this reaction 14% of 6-(methylamino)-N-[4-({[8-methyl-2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]nicotinamide was also isolated. MS (ESI) m/z 428.3.
Step 1: To a stirred solution of 6-bromo-2,4-dichloro-8-methylquinazoline, (290 mg, 1 mmol) in CH2Cl2 N-[4-(aminomethyl)phenyl]-4-chlorobenzamide (259 mg, 0.1 mmol) was added in the presence of triethylamine (5 mL) at room temperature. The reaction mixture was stirred for 8 h and quenched with ice cold water. It was extracted with chloroform and washed well with water. Organic layer was dried and concentrated. The resultant product N-(4-{[(6-bromo-2-chloro-8-methylquinazolin-4-yl)amino]methyl}phenyl)-4-chlorobenzamide was purified by silica gel column chromatography by eluting with 1:1 ethylacetate; hexane. Yield, 260 mg, 51%; MS (ESI) m/z 516.2.
Step 2: A mixture of N-(4-{[(6-bromo-2-chloro-8-methylquinazolin-4-yl)amino]methyl}phenyl)-4-chlorobenzamide (600 mg, 1.2 mmol) and monomethylamine (2M. solution in THF) was heated in a sealed tube at 100° C. for 24 h. At the end, reaction mixture was concentrated and extracted with 3:1 (CHCl3: MeOH). Organic layer was washed once with water and dried over anhydrous MgSO4. It was filtered and concentrated. The solid obtained was suspended in ethylacetate and filtered. The product obtained was found to be pure enough for further transformations. Yield, 450 mg, 68%; MS (ESI) m/z 510.1 mp 139° C.
A mixture of N-[4-({[6-bromo-8-methyl-2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]-4-chlorobenzamide (150 mg, 0.29 mmol) and the 2-(tributylstannyl)furan (357 mg, 1 mmol) and tetrakis(triphenylphosphine)palladium (0) (50 mg) was refluxed in degassed toluene (100 mL) for 48 hrs. At the end, reaction mixture was filtered through a pad of Diatomaceous earth and concentrated. The crude product was purified by silica-gel column chromatography by eluting it with 5% methanol: ethylacetate. Brown solid, Yield, 50 mg (34%), MS (ESI) m/z 498.1.
Starting from the 6-iodo-2,4-dichloroquinazoline (975 mg, 3.0 mmol) and N-[4-(aminomethyl)phenyl]-4-fluorobenzamide (732 mg, 3.0 mmol) and following the procedure outlined for the example 66, step 1, N-(4-{[(2-chloro-6-iodoquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide was isolated as an amorphous solid. Yield, 900 mg, 56%; MS (ESI) m/z 533.2.
Starting from N-(4-{[(2-chloro-6-iodoquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (900 mg, 1.7 mmol), N,N-dimethyl amine (40% solution in THF) and following the procedure outlined for the example 66, step 2, N-[4-({[2-(dimethylamino)-6-iodoquinazolin-4-yl]amino}methyl)phenyl]-4-fluorobenzamide was isolated as an yellow solid. Yield, 800 mg, 87%: MS (ESI) m/z 542.2.
A mixture of N-[4-({[2-(dimethylamino)-6-iodoquinazolin-4-yl]amino}methyl)phenyl]-4-fluorobenzamide (100 mg, 0.18 mmol), Dichlorobis(triphenylphosphine)palladium(II) (PdCl2(PPh3)2) (60 mg), copper iodide (CuI) (100 mg), 3-dimethylamino-1-propyne (100 mg, excess) and triethylamine (4 ml) was refluxed together in acetonitrile for 12 h. At the end, reaction mixture was filtered through a pad of diatomaceous earth and concentrated. The residue was dissolved in chloform; methanol (3:1) and washed once with water. The crude product was purified by silica-gel column chromatography by eluting it with 10% methanol/ethylacetate. Yield, 64 mg, 72%; MS (ESI) m/z 497.3.
To a stirred refluxing solution of N-[4-({[2-(dimethylamino)-6-iodoquinazolin-4-yl]amino}methyl)phenyl]-4-fluorobenzamide (100 mg, 0.18 mmol), PdCl2(PPh3)2 (60 mg) and triethylamine (5 mL) in methanol (50 mL), carbon monoxide (CO) was passed for 48 hrs in a balloon. At the end, reaction mixture was filtered through a pad of celite and concentrated. The residue was purified through a silica-gel column chromatography by eluting it with 10% methanol/ethylacetate. Yield, 40 mg, 46%; MS (ESI) m/z 474.1.
To a stirred suspension of LiAlH4 (60 mg) in THF at 0° C., a THF solution of 1methyl 2-(dimethylamino)-4-({4-[(4-fluorobenzoyl)amino]benzyl}amino)quinazoline-6-carboxylate (200 mg, 0.42 mmol) was slowly added. After the addition, reaction mixture was stirred for 1 h and quenched with ice cold water. The product was extracted with chloroform and washed well water. It was dried over anhydrous MgSO4; filtered and concentrated. Product was purified by silica-gel column chromatography by eluting it with 10% methanol/ethylacetate along with 5 mL/lit 30% NH4OH. Yield, 80 mg, 43%; MS (ESI) m/z 446.3.
Starting from the 6-iodo-2,4-dichloroquinazoline (1625 mg, 5 mmol) and N-[4-(aminomethyl)phenyl]-6-chloronicotinamide (1300 mg, 5.0 mmol) and following the procedure outlined for the example 66, step 1, 6-chloro-N-(4-{[(2-chloro-6-iodoquinazolin-4-yl)amino]methyl}phenyl)nicotinamide was isolated as an amorphous solid. Yield, 1.5 g, 54%; MS (ESI) m/z 550.1.
Starting from 6-chloro-N-(4-{[(2-chloro-6-iodoquinazolin-4-yl)amino]methyl}phenyl)nicotinamide (2000 mg, 3.6 mmol), N,N-dimethylamine (40% solution in THF) and following the procedure outlined for the example 66, step 2, 6-chloro-N-[4-({[2-(dimethylamino)-6-iodoquinazolin-4-yl]amino}methyl)phenyl]nicotinamide was isolated as an yellow solid. Yield, 2000 mg, 98%: MS (ESI) m/z 559.1.
A mixture of 6-chloro-N-[4-({[2-(dimethylamino)-6-iodoquinazolin-4-yl]amino}methyl)phenyl]nicotinamide (300 mg, 0.53 mmol), n-tributylvinyl tin (500 mg, excess) and tetrakis(triphenylphosphine)palladium (0) (50 mg) was heated in Dimethylformamide (DMF) at 100° C. for 8 h. After the completion, reaction mixture was filtered through a pad of diatomaceous earth and concentrated. The residue obtained was extracted with chloroform and washed well with water. Organic layer was dried over anhydrous MgSO4; filtered and concentrated. The crude product was purified by silica-gel column chromatography. Initially the column was eluted with ethylacetate and latter with 20% methanol/ethylacetate and 1.5% aqueous ammonium hydroxide (NH4OH) solution. Yield 190 mg, 78%; MS (ESI) m/z 459.2.
Starting from the 6-iodo-2,4-dichloroquinazoline (650 mg, 2 mmol) and N-[4-(aminomethyl)phenyl]-1-benzylpiperidine-4-carboxamide (646 mg, 2.0 mmol) and following the procedure outlined for the example 66, step 1, 1-benzyl-N-(4-{[(2-chloro-6-iodoquinazolin-4-yl)amino]methyl}phenyl)piperidine-4-carboxamide was isolated as an amorphous solid. Yield, 700 mg, 57%; MS (ESI) m/z 611.9.
Starting from 1-benzyl-N-(4-{[(2-chloro-6-iodoquinazolin-4-yl)amino]methyl}phenyl)piperidine-4-carboxamide (620 mg, 1.0 mmol), N,N-dimethylamine (40% solution in THF) and following the procedure outlined for the example 66, step 2, 1-benzyl-N-[4-({[2-(dimethylamino)-6-iodoquinazolin-4-yl]amino}methyl)phenyl]piperidine-4-carboxamide was isolated as an yellow solid. Yield, 600 mg, 96%: MS (ESI) m/z 621.5.
A mixture of 1-benzyl-N-[4-({[2-(dimethylamino)-6-iodoquinazolin-4-yl]amino}methyl)phenyl]piperidine-4-carboxamide (220 mg, 0.35 mmol), n-tributylvinyl tin (200 mg, excess) and tetrakis(triphenylphosphine)palladium (0) (30 mg) was heated in DMF (25 mL) at 100° C. for 8 h. After the completion, reaction mixture was filtered through a pad of diatomaceous earth and concentrated. The residue obtained was extracted with chloroform and washed well with water. Organic layer was dried over anhydrous MgSO4; filtered and concentrated. The crude product was purified by silica-gel column chromatography. Initially the column was eluted with ethylacetate and latter with 20% methanol/ethylacetate and 1.5% aqueous NH4OH solution. Yield 43 mg, 25%; MS (ESI) m/z 521.5.
Starting from the 8-methyl-2,4-dichloroquinazoline (100 mg, 0.47 mmol) and N-[4-(aminomethyl)phenyl]-1-benzylpiperidine-4-carboxamide (200 mg, excess) and following the procedure outlined for the example 66, step 1, 1-benzyl-N-(4-{[(2-chloro-8-methylquinazolin-4-yl)amino]methyl}phenyl)piperidine-4-carboxamide was isolated as an amorphous solid. Yield, 100 mg, 42%; MS (ESI) m/z 500.3.
Starting from 1-benzyl-N-(4-{[(2-chloro-8-methylquinazolin-4-yl)amino]methyl}phenyl)piperidine-4-carboxamide (100 mg, 0.2 mmol), N,N-dimethylamine (40% solution in THF) and following the procedure outlined for the example 66, step 2, 1-benzyl-N-[4-({[2-(dimethylamino)-8-methylquinazolin-4-yl]amino}methyl)phenyl]piperidine-4-carboxamide was isolated as an yellow solid. Yield, 25 mg, 25%: MS (ESI) m/z 509.6.
Starting from the 6-methyl-2,4-dichloroquinazoline (426 mg, 2.0 mmol) and N-[4-(aminomethyl)phenyl]-1-benzylpiperidine-4-carboxamide (646 mg, 2.0 mmol) and following the procedure outlined for the example 66, step 1, 1-benzyl-N-(4-{[(2-chloro-6-methylquinazolin-4-yl)amino]methyl}phenyl)piperidine-4-carboxamide was isolated as a white solid. Yield, 500 mg, 50%; MS (ESI) m/z 500.3.
Starting from 1-benzyl-N-(4-{[(2-chloro-6-methylquinazolin-4-yl)amino]methyl}phenyl)piperidine-4-carboxamide (30 mg, 0.06 mmol), pyrrolidine (15 mg, excess) and following the procedure outlined for the example 66, step 2, 1-benzyl-N-(4-{[(6-methyl-2-pyrrolidin-1-ylquinazolin-4-yl)amino]methyl}phenyl)piperidine-4-carboxamide was isolated. The product was purified by High performance liquid chromatography (HPLC) using acetonitrile/water/NH4OH-gradient as eluent. MS (ESI) m/2 268.1.
Starting from 1-benzyl-N-(4-{[(2-chloro-6-methylquinazolin-4-yl)amino]methyl}phenyl)piperidine-4-carboxamide (30 mg, 0.06 mmol), 2,6-dimethylpiperazine (20 mg, excess) and following the procedure outlined for the example 66, step 2, 1-benzyl-N-{4-[({2-[(3R,5S)-3,5-dimethylpiperazin-1-yl]-6-methylquinazolin-4-yl}amino)methyl]phenyl}piperidine-4-carboxamide was isolated. The product was purified by HPLC using acetonitrile/water/NH4OH-gradient as eluent. MS (ESI) m/z 578.
Starting from 1-benzyl-N-(4-{[(2-chloro-6-methylquinazolin-4-yl)amino]methyl}phenyl)piperidine-4-carboxamide (30 mg, 0.06 mmol), 1-(2-pyridyl)piperazine (20 mg, excess) and following the procedure outlined for the example 66, step 2, 1-benzyl-N-[4-({[6-methyl-2-(4-pyridin-2-ylpiperazin-1-yl)quinazolin-4-yl]amino}methyl)phenyl]piperidine-4-carboxamide was isolated. The product was purified by HPLC using acetonitrile/water/NH4OH-gradient as eluent. MS (ESI) m/z 627.
Starting from 1-benzyl-N-(4-{[(2-chloro-6-methylquinazolin-4-yl)amino]methyl}phenyl)piperidine-4-carboxamide (30 mg, 0.06 mmol), pyrroline (20 mg, excess) and following the procedure outlined for the example 66, step 2, 1-benzyl-N-[4-({[2-(2,5-dihydro-1H-pyrrol-1-yl)-6-methylquinazolin-4-yl]amino}methyl)phenyl]piperidine-4-carboxamide was isolated. The product was purified by HPLC using acetonitrile/water/NH4OH-gradient as eluent. MS (ESI) m/z 533.
Starting from 1-benzyl-N-(4-{[(2-chloro-6-methylquinazolin-4-yl)amino]methyl}phenyl)piperidine-4-carboxamide (30 mg, 0.06 mmol), furylamine (20 mg, excess) and following the procedure outlined for the example 66, step 2, 1-benzyl-N-{4-[({2-[(2-furylmethyl)amino]-6-methylquinazolin-4-yl}amino)methyl]phenyl}piperidine-4-carboxamide was isolated. The product was purified by HPLC using acetonitrile/water/NH4OH-gradient as eluent. MS (ESI) m/z 561.
Starting from 1-benzyl-N-(4-{[(2-chloro-6-methylquinazolin-4-yl)amino]methyl}phenyl)piperidine-4-carboxamide (30 mg, 0.06 mmol), 1-(2-pyrimidyl)piperazine dihydrochloride (30 mg, excess) and following the procedure outlined for the example 66, step 2, 1-benzyl-N-[4-({[6-methyl-2-(4-pyrimidin-2-ylpiperazin-1-yl)quinazolin-4-yl]amino}methyl)phenyl]piperidine-4-carboxamide was isolated. The product was purified by HPLC using acetonitrile/water/NH4OH-gradient as eluent. MS (ESI) m/z 628.
Starting from 1-benzyl-N-(4-{[(2-chloro-6-methylquinazolin-4-yl)amino]methyl}phenyl)piperidine-4-carboxamide (30 mg, 0.06 mmol), 4-(1-pyrrolidinyl)piperidine (30 mg, excess) and following the procedure outlined for the example 66, step 2, 1-benzyl-N-[4-({[6-methyl-2-(4-pyrrolidin-1-ylpiperidin-1-yl)quinazolin-4-yl]amino}methyl)phenyl]piperidine-4-carboxamide was isolated. The product was purified by HPLC using acetonitrile/water/NH4OH-gradient as eluent. MS (ESI) m/z 618.
Starting from 1-benzyl-N-(4-{[(2-chloro-6-methylquinazolin-4-yl)amino]methyl}phenyl)piperidine-4-carboxamide (30 mg, 0.06 mmol), azetidine hydrochloride (30 mg, excess) and following the procedure outlined for the example 66, step 2, N-(4-{[(2-azetidin-1-yl-6-methylquinazolin-4-yl)amino]methyl}phenyl)-1-benzylpiperidine-4-carboxamide was isolated. The product was purified by HPLC using acetonitrile/water/NH4OH-gradient as eluent. MS (ESI) m/z 521.
Starting from 1-benzyl-N-(4-{[(2-chloro-6-methylquinazolin-4-yl)amino]methyl}phenyl)piperidine-4-carboxamide (30 mg, 0.06 mmol), 2-methylpiperazine (30 mg, excess) and following the procedure outlined for the example 66, step 2, 1-benzyl-N-{4-[({6-methyl-2-[(3R)-3-methylpiperazin-1-yl]quinazolin-4-yl}amino)methyl]phenyl}piperidine-4-carboxamide was isolated. The product was purified by HPLC using acetonitrile/water/NH4OH-gradient as eluent. MS (ESI) m/z 564.
To a solution of 3-iodoaniline (11.4 g, 52 mmol) in CH2Cl2 (150 mL) was added a solution of ethyl isothiocyanatoformate (6.14 mL, 52 mmol) in CH2Cl2 (20 mL). The mixture was stirred at RT for 2 h, and concentrated under reduced pressure to give off-white solid (18.2 g, 100% yield). MS (ESI) m/z 351.1.
To a solution of ethyl {[(3-iodophenyl)amino]carbonothioyl}carbamate (18.2 g, 52 mmol) in acetone (200 mL) was added dropwise of EtI (4.16 mL, 52 mmol) and K2CO3 (21.5 g, 156 mmol). The resulting mixture was stirred at RT overnight, and filtered through a pad of Diatomaceous earth. The filtration was concentrated in vacuum, and the residue was dissolved in CH2Cl2 (200 mL). The solution was washed with water and brine, dried over (MgSO4). Evaporation of solvent gave yellow oil (19.0 g), which was dissolved in phenyl ether (200 mL). The mixture was heated at 200° C. overnight, and cooled to RT, during which time, a lot of white precipitate was formed. The mixture was diluted with hexanes, and filtered to give the title compound as off-white solid (10.8 g, 63% yield). MS (ESI) m/z 333.1.
To a solution of 2-(ethylthio)-7-iodoquinazolin-4(3H)-one (10.8 g, 32.5 mmol) in ethanol (40 mL) was added 6N HCl (40 mL). The mixture was heated at 80° C. overnight, then cooled down to RT, during which time, a lot of white precipitate was formed. The resulting solid was collected by filtration to give off-white solid (8.97 g, 96% yield). MS (ESI) m/z 289.0.
POCl3 (10 mL) was added to 7-iodoquinazoline-2,4(1H,3H)-dione (3.78 g, 13.1 mmol), followed by addition of N,N-dimethylaniline (1 mL). The resulting mixture was heated at 115° C. for 6 h. After cooling to RT, Most of POCl3 was removed by distillation under reduced pressure. The residue was poured into ice-water, ammonium hydroxide was added to adjust pH to 5-7. The mixture was extracted several times with CH2Cl2, and the combined extracts were washed with brine, and dried over (MgSO4). The solvent was removed under reduced pressure, and the residue was purified by flash chromatography (Ethyl acetate (EtOAc):CH2Cl2=5:95) to give 2,4-dichloro-7-iodoquinazoline as off-white solid (3.56 g, 84%); Melting point (Mp): 163° C.; MS (ESI) m/z 324.9.
To a solution of N-[4-(aminomethyl)phenyl]-4-fluorobenzamide (537 mg, 2.2 mmol) in CH2Cl2 (10 mL) was added Et3N (0.84 mL, 6.0 mmol), followed by addition of 2,4-dichloro-7-iodoquinazoline (648 mg, 2.0 mmol). The resulting mixture was stirred at RT for 20 h, during which time a lot of precipitate was formed. The resulting solid was collected by filtration and washed with small amount of EtOAc and water to give the expected product as off-white solid (745 mg, yield: 70%). MS (ESI) m/z 533.0.
To a solution of N-(4-{[(2-chloro-7-iodoquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (532 mg, 1 mmol) in THF (2 mL) was added dimethylamine solution (40% in water, 0.6 mL, 5 mmol). The resulting mixture was heated at 100° C. in a sealed tube for 26 h, then cooled to RT. The solvent was evaporated under reduced pressure, and the residue was purified by flash chromatography (CH2Cl2:CH3OH=90:10) to give off-white solid (487 mg, yield: 90%). MS (ESI)m/z 542.1.
To a solution of N-[4-({[2-(dimethylamino)-7-iodoquinazolin-4-yl]amino}methyl)phenyl]-4-fluorobenzamide (541 mg, 1 mmol) in DMF (5 mL), was added PdCl2(PPh3)2 (35 mg, 5 mol %) as catalyst, followed by addition of tributyl(vinyl)tin (0.35 mL, 1.2 mmol). The resulting mixture was heated at 90° C. under nitrogen for 3 h. Upon completion, the reaction mixture was cooled down to RT, and then poured into cold water. The resulting solid was collected by filtration, and then purified by flash chromatography (CH2Cl2:CH3OH=90:10) to give off-white solid, which was treated with HCl in methanol to form HCl salt as off-white solid (292 mg, yield: 61%). Mp: 145° C.; MS (ESI) m/z 442.1.
To a solution of N-[4-({[2-(dimethylamino)-7-vinylquinazolin-4-yl]amino}methyl)phenyl]-4-fluorobenzamide (239 mg, 0.5 mmol) in MeOH (10 mL) was added Pd/C (10%) catalyst (48 mg). The resulting mixture was hydrogenated at RT for 2 h. The reaction mixture was filtered through a pad of Diatomaceous earth, washed with methanol, and the resulting filtrate was concentrated under reduced pressure. The residue was subjected to HPLC separation to give off-white solid, which was converted to HCl salt as off-white solid (229 mg, yield: 96%). Mp: 270° C.; MS (ESI) m/z 444.2.
To a solution of N-[4-({[2-(dimethylamino)-7-iodoquinazolin-4-yl]amino}methyl)phenyl]-4-fluorobenzamide (252 mg, 0.46 mmol) in DMF 9 (mL) were added zinc cyanide (Zn(CN)2) (66 mg, 0.56 mmol) and tetrakis(triphenylphosphine)palladium(0) (Pd(PPh3)4) (26 mg, 5 mol %). The resulting mixture was heated at 80° C. under N2 for 5 h. Upon completion, the reaction mixture was cooled to RT, and poured into cold water. The resulting solid was collected by filtration, and purified by HPLC, and then converted to HCl salt to give off-white solid (156 mg, 70%). Mp: 150° C.; MS (ESI) m/z 441.1.
To a solution of N-[4-({[7-cyano-2-(dimethylamino)quinazolin-4-yl]amino}methyl)phenyl]-4-fluorobenzamide (142 mg, 0.32 mmol) in MeOH (10 mL) was added Pd/C (10%) catalyst (28 mg) and concentrated (Conc.) HCl (30%, 0.2 mL). The resulting mixture was hydrogenated at RT for 48 h. The reaction mixture was filtered through a pad of Diatomaceous earth, washed with methanol, and the resulting filtrate was concentrated under reduced pressure to give off-white solid (HCl salt, 140 mg, yield: 96%). Mp: 245° C.; MS (ESI) m/z 445.4.
To a solution of N-[4-({[7-(aminomethyl)-2-(dimethylamino)quinazolin-4-yl]amino}methyl)phenyl]-4-fluorobenzamide HCl salt (110 mg, 0.21 mmol) in MeOH (2 mL) were added formaldehyde (37%, 68 μL, 0.84 mmol), NaBH3CN (13 mg, 0.21 mmol) and ZnCl2 (14 mg, 0.10 mmol). The resulting mixture was stirred at RT overnight. The mixture was filtered, and washed with methanol. The resulting filtrate was concentrated in vacuum, and subjected to HPLC separation, and then converted the product to HCl salt to give off-white solid (68 mg, 60%); Mp: 94° C.; MS (ESI) m/z 473.3.
To a solution of N-[4-({[2-(dimethylamino)-7-iodoquinazolin-4-yl]amino}methyl)phenyl]-4-fluorobenzamide (417 mg, 0.75 mmol) in DMF 3 (mL) were added sodium formate (HCO2Na) (102 mg, 1.5 mmol) and PdCl2(PPh3)2 (26 mg, 5 mol %). The resulting mixture was heated at 100° C. under CO (gas, 1 atm) for 5 h. Upon completion, the reaction mixture was cooled to RT, and poured into cold water. The resulting solid was collected by filtration, and purified by HPLC to give off-white solid (123 mg, 37%). MS (ESI) m/z 444.1.
To a solution of N-[4-({[2-(dimethylamino)-7-formylquinazolin-4-yl]amino}methyl)phenyl]-4-fluorobenzamide (100 mg, 0.23 mmol) in MeOH (5 mL) was added sodium borohydride (NaBH4) (17 mg, 0.46 mmol). The resulting mixture was stirred at RT for 2 h. The reaction mixture was concentrated under reduced pressure, and the residue was treated with sat. Sodium bicarbonate (NaHCO3) aqueous solution and extracted with ethyl acetate (EtOAc). The organic phases were washed with brine and dried over (MgSO4). The solvent was removed and the residue was purified by HPLC and converted to HCl salt to give off-white solid (94 mg, 85%); Mp: 158° C.; MS (ESI) m/z 446.2.
To a solution of N-[4-({[2-(dimethylamino)-7-iodoquinazolin-4-yl]amino}methyl)phenyl]-4-fluorobenzamide (400 mg, 0.74 mmol) in DMF (5 mL), was added PdCl2(PPh3)2 (26 mg, 5 mol %) as catalyst, followed by addition of tributyl(1-ethoxyvinyl)tin (0.27 mL, 0.81 mmol). The resulting mixture was heated at 90° C. under nitrogen for 4 h. Upon completion, the reaction mixture was cooled down to RT, and then poured into cold water. The resulting solid was collected by filtration. The solid was dissolved in MeOH (3 mL) and 6N HCl (0.5 mL), and heated at 70° C. for 3 h. The mixture was cooled to RT, and concentrated in vacuum. The resulting residue was purified by flash chromatography (CH2Cl2:CH3OH=90:10) to give off-white solid (168 mg, yield: 50%). Mp: 195° C.; MS (ESI) m/z 458.2.
To a solution of N-[4-({[7-acetyl-2-(dimethylamino)quinazolin-4-yl]amino}methyl)phenyl]-4-fluorobenzamide (84 mg, 0.18 mmol) in MeOH (5 mL) was added NaBH4 (14 mg, 0.36 mmol). The resulting mixture was stirred at RT for 6 h. The reaction mixture was concentrated under reduced pressure, and the residue was treated with sat. NaHCO3 aqueous solution and extracted with EtOAc). The organic phases were washed with brine, and dried over (MgSO4). The solvent was removed, and the residue was purified by HPLC, and converted to HCl salt to give off-white solid (71 mg, 80%); Mp: 232° C.; MS (ESI) m/z 460.2.
The compound was prepared from N-[4-({[2-(dimethylamino)-7-iodoquinazolin-4-yl]amino}methyl)phenyl]-4-fluorobenzamide (200 mg, 0.37 mmol) and (E)-tributyl(1-propenyl)tin by following the same procedure as Example 87 (Stille coupling) as off-white solid (HCl salt, 138 mg) in 76% yield. Mp: 268° C.; MS (ESI) m/z 456.1.
A glass tube was charged with N-[4-({[2-(dimethylamino)-7-iodoquinazolin-4-yl]amino}methyl)phenyl]-4-fluorobenzamide (200 mg, 0.37 mmol), (Z)-1-propenylboronic acid (48 mg, 0.55 mmol), Pd(PPh3)4 (23 mg, 5 mol %), DME (3 mL) and Sat. NaHCO3 (2 mL). The resulting mixture was heated at 100° C. for 15 min under N2 in microwave, and then cooled to RT. Filtered, washed with methanol, and the filtrate was concentrated in vacuum to give a solid, which was subjected to HPLC separation. The product was converted to HCl salt to give off-white solid (119 mg, 65%). Mp: 246° C.; MS (ESI) m/z 456.2.
The compound was prepared from] N-[4-({[2-(dimethylamino)-7-iodoquinazolin-4-yl]amino}methyl)phenyl]-4-fluorobenzamide (100 mg, 0.18 mmol) and 2-formylphenylboronic acid (32 mg, 0.22 mmol) by following the same procedure as Example 97 (Suzuki coupling) as brown solid (HCl salt, 48 mg) in 48% yield. Mp: 128° C.; MS (ESI) m/z 520.2.
The compound was prepared from N-[4-({[2-(dimethylamino)-7-iodoquinazolin-4-yl]amino}methyl)phenyl]-4-fluorobenzamide (100 mg, 0.18 mmol) and 4-formylphenylboronic acid (38 mg, 0.25 mmol) by following the same procedure as Example 97 (Suzuki coupling) as off-white solid (HCl salt, 47 mg) in 47% yield. MS (ESI) m/z 520.2.
The compound was prepared from N-[4-({[2-(dimethylamino)-7-iodoquinazolin-4-yl]amino}methyl)phenyl]-4-fluorobenzamide (100 mg, 0.18 mmol) and 2-chloropyridin-3-ylboronic acid (40 mg, 0.25 mmol) by following the same procedure as Example 97 (Suzuki coupling) as off-white solid (HCl salt, 56 mg) in 55% yield. MS (ESI) m/z 527.2.
The compound was prepared from N-[4-({[2-(dimethylamino)-7-iodoquinazolin-4-yl]amino}methyl)phenyl]-4-fluorobenzamide (100 mg, 0.18 mmol) and 1-benzofuran-2-ylboronic acid (40 mg, 0.25 mmol) by following the same procedure as Example 97 (Suzuki coupling) as off-white solid (HCl salt, 22 mg) in 21% yield. MS (ESI) m/z 532.2.
The compound was prepared from N-[4-({[2-(dimethylamino)-7-iodoquinazolin-4-yl]amino}methyl)phenyl]-4-fluorobenzamide (150 mg, 0.28 mmol) and (E)-3,3-dimethylbut-1-enylboronic acid (53 mg, 0.42 mmol) by following the same procedure as Example 97 (Suzuki coupling) as off-white solid (HCl salt, 40 mg) in 27% yield. MS (ESI) m/z 498.3.
The compound was prepared from N-[4-({[2-(dimethylamino)-7-iodoquinazolin-4-yl]amino}methyl)phenyl]-4-fluorobenzamide (120 mg, 0.22 mmol) and (E)-hex-1-enylboronic acid (70 mg, 0.55 mmol) by following the same procedure as Example 97 (Suzuki coupling) as off-white solid (HCl salt, 65 mg) in 55% yield. MS (ESI) m/z 498.3.
The compound was prepared from N-[4-({[2-(dimethylamino)-7-iodoquinazolin-4-yl]amino}methyl)phenyl]-4-fluorobenzamide (100 mg, 0.18 mmol) and cyclopropylboronic acid (24 mg, 0.28 mmol) and by following the same procedure as Example 97 (Suzuki coupling) as off-white solid (HCl salt, 32 mg) in 36% yield. MS (ESI) m/z 456.1.
Starting from N-{4-[(1S)-1-aminoethyl]phenyl}-6-chloronicotinamide (220 mg, 0.8 mmol) and 2,4-dichloro-7-iodoquinazoline (285 mg, 0.88 mmol) by following the same procedure as Example 86 (step 5), 6-chloro-N-(4-{(1S)-1-[(2-chloro-7-iodoquinazolin-4-yl)amino]ethyl}phenyl)nicotinamide was isolated as off-white solid (460 mg, 99% yield). MS (ESI) m/z 564.2.
Starting from 6-chloro-N-(4-{(1S)-1-[(2-chloro-7-iodoquinazolin-4-yl)amino]ethyl}phenyl)nicotinamide (430 mg, 0.76 mmol) and dimethylamine hydrochloride (311 mg, 3.8 mmol) and following the same procedure as Example 86 (step 6), 6-chloro-N-[4-((1S)-1-{[2-(dimethylamino)-7-iodoquinazolin-4-yl]amino}ethyl)phenyl]nicotinamide was isolated as off-white solid (395 mg, 85% yield). HRMS: calcd for C24H22ClIN6O+H+, 573.06611; found (ESI-FTMS, [M+H]1+), 573.06749.
6-chloro-N-[4-((1S)-1-{[2-(dimethylamino)-7-vinylquinazolin-4-yl]amino}ethyl)phenyl]nicotinamide was prepared from 6-chloro-N-[4-((1S)-1-{[2-(dimethylamino)-7-iodoquinazolin-4-yl]amino}ethyl)phenyl]nicotinamide (170 mg, 0.3 mmol) and tributyl(vinyl)tin (105 mg, 0.33 mmol) by following the same procedure as in Example 87 (Stille coupling) to give the product as yellow solid (82 mg, 58% yield). MS (ESI) m/z 473.3; HRMS: calcd for C26H25ClN6O+H+, 473.18511; found (ESI-FTMS, [M+H]1+), 473.18605.
6-chloro-N-{4-[(1S)-1-({2-(dimethylamino)-7-[(1E)-prop-1-en-1-yl]quinazolin-4-yl}amino)ethyl]phenyl}nicotinamide was prepared from 6-chloro-N-[4-((1S)-1-{[2-(dimethylamino)-7-iodoquinazolin-4-yl]amino}ethyl)phenyl]nicotinamide (170 mg, 0.3 mmol) and (E)-tributyl(1-propenyl)tin (119 mg, 0.36 mmol) by following the same procedure as in Example 87 (Stille coupling) to give the product as yellow solid (39 mg, 27% yield). MS (ESI) m/z 487.3; HRMS: calcd for C27H27ClN6O+H+, 487.20076; found (ESI-FTMS, [M+H]1+), 487.20171.
To a solution of N-[4-({[2-(dimethylamino)-7-iodoquinazolin-4-yl]amino}methyl)phenyl]-4-fluorobenzamide (150 mg, 0.28 mmol) in DMF (3 mL) were added ethynyltrimethylsilane (80 mg, 0.56 mmol), PdCl2(PPh3)2 (10 mg, 5 mol %), CuI (3 mg, 5 mol %) and Et3N (0.12 mL, 0.84 mmol). The resulting mixture was stirred at RT under N2 for 3 h. Upon completion, the reaction mixture was cooled to RT, and poured into cold water. The resulting solid was collected by filtration.
The solid was stirred with KOH (1M in MeOH, 2 mL) at RT overnight. The reaction mixture was adjusted pH to 5-6 by addition of 1N HCl, and then extracted with EtOAc. The combined organic phases was washed with brine, and dried over MgSO4. The solvent was removed under reduced pressure, and the residue was purified by flash chromatography (EtOAc:CH2Cl2=70:30) to give off-white solid, which was treated with 4N HCl dioxane solution to give HCl salt as off-white solid (68 mg, 51%). Mp: 250° C.; MS (ESI) m/z 440.2.
The compound was prepared from 2,4-dichloro-7-iodoquinazoline (648 mg, 2 mmol) and N-[4-(aminomethyl)phenyl]-6-chloronicotinamide (522 mg, 2 mmol) by following the same procedure as Example 86 (step 5) as off-white solid (936 mg) in 85% yield; Mp: 315° C.; MS (ESI) m/z 550.1.
Step 1: Starting from 2,4-dichloro-7-iodoquinazoline (648 mg, 2 mmol) and N-[4-(aminomethyl)phenyl]-6-chloronicotinamide (522 mg, 2 mmol) by following the same procedure as Example 87 (step 5), 6-chloro-N-(4-{[(2-chloro-7-iodoquinazolin-4-yl)amino]methyl}phenyl)nicotinamide was isolated as off-white solid (936 mg) in 85% yield; Mp: 315° C.; MS (ESI) m/z 550.1.
Step 2: To a solution of 6-chloro-N-(4-{[(2-chloro-7-iodoquinazolin-4-yl)amino]methyl}phenyl)nicotinamide (400 mg, 0.73 mmol) in DMF (2 mL) was added dimethylamine hydrochloride (279 mg, 3.64 mmol). The mixture was heated at 120° C. for 10 min in microwave, and cooled to RT. The reaction mixture was poured into cold water, and the resulting solid was collected by filtration. After drying, the solid was treated with hot ethanol, then cooled to RT, filtered, and washed with cold ethanol. The title compound was obtained as off-white solid (HCl salt, 375 mg) in 86% yield. Mp: 170° C.; MS (ESI) m/z 559.1.
Method A: The compound was prepared from 6-chloro-N-[4-({[2-(dimethylamino)-7-iodoquinazolin-4-yl]amino}methyl)phenyl]nicotinamide (150 mg, 0.27 mmol) by following the same procedure as Example 87 (Stille coupling) as off-white solid (HCl salt, 61 mg) in 46% yield. Mp: 290° C.; MS (ESI) m/z 459.2.
Method B: Step 1: Synthesis of 2,4-dichloro-7-vinylquinazoline. To a suspension of 7-iodoquinazoline-2,4(1H,3H)-dione (5.0 g, 17.4 mmol) in DMF (20 mL) was added PdCl2(PPh3)2 (609 mg, 5 mol %) as catalyst, followed by addition of tributyl(vinyl)tin (6.1 mL, 20.9 mmol). The resulting mixture was heated at 105° C. under nitrogen for 30 min in microwave. Upon completion, the reaction mixture was cooled down to RT, and then poured into cold water. The resulting solid was collected by filtration, and dried in vacuum to give a brown solid (4.5 g). To the solid was added POCl3 (40 mL), and the resulting mixture was heated at 115° C. for 3 h. After cooling to RT, most of POCl3 was removed by distillation under reduced pressure. The residue was poured into ice-water, ammonium hydroxide was added to adjust pH to 5-7. The mixture was extracted several times with CH2Cl2, and the combined extracts were washed with brine, and dried over (MgSO4). The solvent was removed under reduced pressure, and the residue was purified by flash chromatography (EtOAc:Hexane=10:90) to give as off-white solid (2.86 g, 74%); MS (ESI) m/z 224.9.
The compound was prepared from 2,4-dichloro-7-vinylquinazoline (400 mg, 1.8 mmol) and N-[4-(aminomethyl)phenyl]-6-chloronicotinamide (522 mg, 2 mmol) by following the same procedure as Example 66 (step 1) as off-white solid (509 mg) in 63% yield. MS (ESI) m/z 450.1.
To a solution of 6-chloro-N-(4-{[(2-chloro-7-vinylquinazolin-4-yl)amino]methyl}phenyl)nicotinamide (250 mg, 0.56 mmol) in DMF (3 mL) was added dimethylamine hydrochloride (227 mg, 2.78 mmol). The mixture was heated at 120° C. for 10 min. in microwave, and cooled to RT. The reaction mixture was poured into cold water, and the resulting solid was collected by filtration. After drying, the solid was treated with hot ethanol. The mixture was cooled to RT, filtered, and washed with cold ethanol. The title compound was obtained as off-white solid (HCl salt, 235 mg) in 85% yield; Mp: 290° C.; MS (ESI) m/z 459.2.
The compound was prepared from 6-chloro-N-[4-({[2-(dimethylamino)-7-iodoquinazolin-4-yl]amino}methyl)phenyl]nicotinamide (150 mg, 0.27 mmol) and (E)-tributyl(1-propenyl)tin (178 mg, 0.54 mmol) by following the same procedure as Example 87 (Stille coupling) as off-white solid (HCl salt, 36 mg) in 26% yield. Mp: 185° C.; MS (ESI) m/z 473.2.
To a solution of N-[4-(aminomethyl)phenyl]-4-fluorobenzamide (1.70 g, 6.97 mmol) in CH2Cl2 (30 mL) was added Et3N (2.6 mL, 18.5 mmol), followed by addition of 2,4-dichloroquinazoline (1.66 g, 8.38 mmol). The resulting mixture was stirred at RT overnight, during which time a lot of precipitate was formed. The resulting solid was collected by filtration and washed with small amount of EtOAc and water to give the expected product as off-white solid (2.43 g, yield: 86%). MS (ESI) m/z 407.3.
To a solution of N-(4-{[(2-chloroquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (100 mg, 0.25 mmol) in THF (2 mL) was added dimethylamine solution (40% in water, 0.32 mL, 2.5 mmol). The resulting mixture was heated at 100° C. in a sealed tube for 21 h, then cooled to RT. The solvent was evaporated under reduced pressure, and the residue was purified by flash chromatography (CH2Cl2:CH3OH=90:10) to give off-white solid (96 mg, yield: 93%). MS (ESI) m/z 416.2.
The compound was prepared from N-(4-{[(2-chloroquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (203 mg, 0.5 mmol) and azetidine hydrochloride (187 mg, 2 mmol) by following the same procedure as Example 66 (step 2) as a white solid (181 mg, 85%). Mp: 249° C.; MS (ESI) m/z 428.3.
The compound was prepared from N-(4-{[(2-chloroquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (120 mg, 0.3 mmol) and cyclobutylamine hydrochloride (323 mg, 3 mmol) by following the same procedure as Example 66 (step 2) as a white solid (77 mg, 58%). MS (ESI) m/z 442.4.
The compound was prepared from N-(4-{[(2-chloroquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (120 mg, 0.3 mmol) and 1-methylpiperazine (0.33 mL, 3 mmol) by following the same procedure as Example 66 (step 2) as a white solid (140 mg, 99%). MS (ESI) m/z 471.4.
The compound was prepared from N-(4-{[(2-chloroquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (120 mg, 0.3 mmol) and morpholine (0.29 mL, 3 mmol) by following the same procedure as Example 66 (step 2) as a white solid (90 mg, 66%). MS (ESI) m/z 458.3.
The compound was prepared from N-(4-{[(2-chloroquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (120 mg, 0.3 mmol) and ethylamine (70% in water, 0.25 mL, 3 mol) by following the same procedure as Example 66 (step 2) as a white solid (97 mg, 78%). MS (ESI) m/z 416.4.
The compound was prepared from N-(4-{[(2-chloroquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (100 mg, 0.25 mmol) and pyrrolidine (0.21 mL, 2.5 mmol) by following the same procedure as Example 66 (step 2) as a white solid (107 mg, 97%). MS (ESI) m/z 442.1.
The compound was prepared from N-(4-{[(2-chloroquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (100 mg, 0.25 mmol) and cyclopentylamine (0.25 mL, 2.5 mmol) by following the same procedure as Example 66 (step 2) as a white solid (55 mg, 48%). MS (ESI) m/z 456.2.
The compound was prepared from N-(4-{[(2-chloroquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (203 mg, 0.5 mmol) and cyclopropylamine (1 mL, 14 mmol) by following the same procedure as Example 66 (step 2) as a white solid (165 mg, 77%). Mp: 189° C.; MS (ESI) m/z 428.3.
The compound was prepared from N-(4-{[(2-chloroquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (100 mg, 0.25 mmol) and diethylamine (0.26 mL, 2.5 mmol) by following the same procedure as Example 66 (step 2) as a white solid (62 mg, 56%). MS (ESI) m/z 444.2.
The compound was prepared from N-(4-{[(2-chloroquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (100 mg, 0.25 mmol) and piperidine (0.25 mL, 2.5 mmol) by following the same procedure as Example 66 (step 2) as a white solid (103 mg, 91%). MS (ESI) m/z 456.5.
The compound was prepared from N-(4-{[(2-chloroquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (100 mg, 0.25 mmol) and furfurylamine (0.23 mL, 2.5 mmol) by following the same procedure as Example 66 (step 2) as a white solid (100 mg, 87%). MS (ESI) m/z 468.4.
The compound was prepared from N-(4-{[(2-chloroquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (100 mg, 0.25 mmol) and cyclohexylamine (0.29 mL, 2.5 mmol) by following the same procedure as Example 66 (step 2) as a white solid (40 mg, 34%). MS (ESI) m/z 470.4.
The compound was prepared from N-(4-{[(2-chloroquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (203 mg, 0.5 mmol) and tert-butyl glycinate (643 mg, 5 mmol) by following the same procedure as Example 66 (step 2) as a white solid (139 mg, 55%). Mp: 96° C.; MS (ESI) m/z 502.3.
To a suspension of tert-butyl N-[4-({4-[(4-fluorobenzoyl)amino]benzyl}amino)quinazolin-2-yl]glycinate (56 mg, 0.9 mmol) in CH2Cl2 (3 mL) was added TFA (1 mL) at RT. The mixture was stirred at RT for 3 h, and concentrated in vacuum. The residue was subjected to HPLC separation and then converted to corresponding HCl salt to give the product as off-white solid (46 mg, 88% yield). Mp: 235° C.; MS (ESI) m/z 446.2.
Step 1: Starting from 2,4-dichloroquinazoline (261 mg, 1.3 mmol) and N-[4-(aminomethyl)phenyl]-6-chloronicotinamide (288 mg, 1.1 mmol) by following the same procedure as Example 66 (step 1), 6-chloro-N-(4-{[(2-chloroquinazolin-4-yl)amino]methyl}phenyl)nicotinamide was isolated as off-white solid (350 mg, 75% yield). MS (ESI) m/z 445.2.
Step 2: Starting from 6-chloro-N-(4-{[(2-chloroquinazolin-4-yl)amino]methyl}phenyl)nicotinamide (150 mg, 0.35 mmol) and dimethylamine (40% in water, 0.23 mL, 1.8 mmol) and following the same procedure as Example 66 (step 2), 6-chloro-N-[4-({[2-(dimethylamino)quinazolin-4-yl]amino}methyl)phenyl]nicotinamide was isolated as off-white solid (80 mg, 53% yield). MS (ESI) m/z 433.3.
At the same time, another product 6-(dimethylamino)-N-[4-({[2-(dimethylamino)quinazolin-4-yl]amino}methyl)phenyl]nicotinamide was also isolated from this reaction as off-white solid (48 mg, 31% yield). MS (ESI) m/z 442.4.
To a stirred solution of N-[4-(aminomethyl)phenyl]-1-benzylpiperidine-4-carboxamide (323 mg, 1 mmol) in CH2Cl2 (10 mL) was added Et3N (0.42 mL, 3 mmol), followed by addition of 2,4-dichloro-7-methylquinazoline (254 mg, 1.2 mmol). The resulting mixture was stirred at RT overnight, and then diluted with CH2Cl2. The mixture was washed with sat. NaHCO3 aqueous solution, dried over (MgSO4). The solvent was removed under reduced pressure, and the residue was purified by flash chromatography (EtOAc:Hexanes:Methanol=70:20:10) to give off-white solid (370 mg, 74%). Mp: 212° C.; MS (ESI) m/z 500.3.
To a solution of 1-benzyl-N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)piperidine-4-carboxamide (120 mg, 0.24 mmol) in THF (3 mL) was added dimethylamine solution (40% in water, 0.3 mL, 2.5 mmol). The resulting mixture was heated at 100° C. in a sealed tube for 26 h, then cooled to RT. The solvent was evaporated under reduced pressure, and the residue was purified by HPLC and converted to its HCl salt to give off-white solid (126 mg, yield: 90%). Mp: 100° C.; MS (ESI) m/z 509.3.
The compound was prepared from 1-benzyl-N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)piperidine-4-carboxamide (120 mg, 0.24 mmol) and methylamine hydrochloride (162 mg, 2.4 mmol) by following the same procedure as Example 129 (step 2) as a white solid (57 mg, 42%). Mp: 128° C.; MS (ESI) m/z 495.4.
The compound was prepared from 1-benzyl-N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)piperidine-4-carboxamide (250 mg, 0.5 mmol) and hexamethyleneimine (0.28 mL, 2.5 mmol) by following the same procedure as Example 129 (step 2) as a white solid (168 mg, 53%). Mp: 66° C.; MS (ESI) m/z 563.7.
The compound was prepared from 1-benzyl-N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)piperidine-4-carboxamide (200 mg, 0.4 mmol) and ethylamine (70% in water, 258 mg, 4 mmol) by following the same procedure as Example 129 (step 2) as a white solid (88 mg, 38%). Mp: 55° C.; MS (ESI) m/z 509.4.
The compound was prepared from 1-benzyl-N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)piperidine-4-carboxamide (200 mg, 0.4 mmol) and pyrrolidine (284 mg, 4 mmol) by following the same procedure as Example 129 (step 2) as a white solid (122 mg, 57%). Mp: 96° C.; MS (ESI) m/z 535.3.
The compound was prepared from 1-benzyl-N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)piperidine-4-carboxamide (30 mg, 0.06 mmol) and azetidine hydrochloride (28 mg, 0.3 mmol) by following the same procedure as Example 129 (step 2) as off-white solid (19 mg, 59%). MS (ESI) m/z 521.6.
The compound was prepared from 1-benzyl-N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)piperidine-4-carboxamide (30 mg, 0.06 mmol) and 4-pyrrolinylpiperidine (46 mg, 0.3 mmol) by following the same procedure as Example 129 (step 2) as off-white solid (27 mg, 71%). MS (ESI) m/z 618.8.
The compound was prepared from 1-benzyl-N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)piperidine-4-carboxamide (30 mg, 0.06 mmol) and 4-pyrimidin-2-ylpiperazine dihydrochloride (71 mg, 0.3 mmol) by following the same procedure as Example 129 (step 2) as off-white solid (19 mg, 50%). MS (ESI) m/z 628.7.
The compound was prepared from 1-benzyl-N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)piperidine-4-carboxamide (30 mg, 0.06 mmol) and 1-ethylpiperazine (34 mg, 0.3 mmol) by following the same procedure as Example 129 (step 2) as off-white solid (19 mg, 54%). MS (ESI) m/z 578.8.
The compound was prepared from 1-benzyl-N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)piperidine-4-carboxamide (30 mg, 0.06 mmol) and 2-(piperazin-2-yl)ethanol (39 mg, 0.3 mmol) by following the same procedure as Example 129 (step 2) as off-white solid (33 mg, 91%). MS (ESI) m/z 594.8.
The compound was prepared from 1-benzyl-N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)piperidine-4-carboxamide (30 mg, 0.06 mmol) and 2-methylaminoethanol (23 mg, 0.3 mmol) by following the same procedure as Example 129 (step 2) as off-white solid (29 mg, 87%). MS (ESI) m/z 539.7.
The compound was prepared from 1-benzyl-N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)piperidine-4-carboxamide (30 mg, 0.06 mmol) and N,N,N′-trimethylpropane-1,3-diamine (35 mg, 0.3 mmol) by following the same procedure as Example 129 (step 2) as off-white solid (26 mg, 73%). MS (ESI) m/z 580.8.
The compound was prepared from 1-benzyl-N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)piperidine-4-carboxamide (30 mg, 0.06 mmol) and 1-methylpiperazine (30 mg, 0.3 mmol) by following the same procedure as Example 129 (step 2) as off-white solid (26 mg, 74%). MS (ESI) m/z 564.7.
The compound was prepared from 1-benzyl-N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)piperidine-4-carboxamide (30 mg, 0.06 mmol) and benzylmethylamine (36 mg, 0.3 mmol) by following the same procedure as Example 129 (step 2) as off-white solid (25 mg, 71%). MS (ESI) m/z 585.6.
The compound was prepared from 1-benzyl-N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)piperidine-4-carboxamide (30 mg, 0.06 mmol) and (R)-2-methylpiperazine (30 mg, 0.3 mmol) by following the same procedure as Example 129 (step 2) as off-white solid (34 mg, 98%). MS (ESI) m/z 564.6.
The compound was prepared from 1-benzyl-N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)piperidine-4-carboxamide (30 mg, 0.06 mmol) and N,N,N′-trimethylethanediamine (31 mg, 0.3 mmol) by following the same procedure as Example 129 (step 2) as off-white solid (26 mg, 76%). MS (ESI) m/z 566.8.
The compound was prepared from 1-benzyl-N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)piperidine-4-carboxamide (30 mg, 0.06 mmol) and methyl(2-pyridinyl)ethylamine (41 mg, 0.3 mmol) by following the same procedure as Example 129 (step 2) as off-white solid (34 mg, 93%). MS (ESI) m/z 600.8.
Step 1: Starting from N-[4-(aminomethyl)phenyl]-1-benzylpiperidine-4-carboxamide (323 mg, 1 mmol) and 2,4-dichloroquinazoline (238 mg, 1.2 mmol) by following the same procedure as Example 129 (step 1), 1-benzyl-N-(4-{[(2-chloroquinazolin-4-yl)amino]methyl}phenyl)piperidine-4-carboxamide was isolated as off-white solid (456 mg, 94%). MS (ESI) m/z 486.4.
Step 2: Starting from 1-benzyl-N-(4-{[(2-chloroquinazolin-4-yl)amino]methyl}phenyl)piperidine-4-carboxamide (100 mg, 0.2 mmol) and dimethylamine (40% in water, 0.25 mL, 2 mmol) and following the same procedure as Example 129 (step 2), 1-benzyl-N-[4-({[2-(dimethylamino)quinazolin-4-yl]amino}methyl)phenyl]piperidine-4-carboxamide was isolated as off-white solid (60 mg, 59% yield). MS (ESI) m/z 495.4.
This compound was prepared from 1-benzyl-N-(4-{[(2-chloroquinazolin-4-yl)amino]methyl}phenyl)piperidine-4-carboxamide (100 mg, 0.2 mmol) and methylamine (2M in THF, 5 mL, 10 mmol) by following the same procedure as Example 129 (step 2) to give the product as off-white solid (50 mg, 51% yield). MS (ESI) m/z 481.4.
Step 1: Starting from N-[4-(aminomethyl)phenyl]-1-benzylpiperidine-4-carboxamide (165 mg, 0.5 mmol) and 2,4-dichloro-6-methylquinazoline (130 mg, 0.6 mmol) by following the same procedure as Example 129 (step 1), 1-benzyl-N-(4-{[(2-chloro-6-methylquinazolin-4-yl)amino]methyl}phenyl)piperidine-4-carboxamide was isolated as off-white solid (139 mg, 55% yield). MS (ESI) m/z 500.5.
Step 2: Starting from 1-benzyl-N-(4-{[(2-chloro-6-methylquinazolin-4-yl)amino]methyl}phenyl)piperidine-4-carboxamide (79 mg, 0.16 mmol) and dimethylamine (40% in water, 0.3 mL, 2.4 mmol) and following the same procedure as Example 129 (step 2), 1-benzyl-N-[4-({[2-(dimethylamino)-6-methylquinazolin-4-yl]amino}methyl)phenyl]piperidine-4-carboxamide was isolated as off-white solid (26 mg, 32% yield). MS (ESI) m/z 509.4.
This compound was prepared from 1-benzyl-N-(4-{[(2-chloro-6-methylquinazolin-4-yl)amino]methyl}phenyl)piperidine-4-carboxamide (143 mg, 0.29 mmol) and methyl amine (2M in THF, 5 mL, 10 mmol) and following the same procedure as Example 129 (step 2) as off-white solid (71 mg, 50% yield). MS (ESI) m/z 495.4.
To a solution of N-[4-(aminomethyl)phenyl]-1-benzylpiperidine-4-carboxamide (355 mg, 1.1 mmol) in CH2Cl2 (10 mL) was added Et3N (0.28 mL, 3.3 mmol), followed by addition of 2,4-dichloro-7-iodoquinazoline (356 mg, 1.1 mmol). The resulting mixture was stirred at RT for 20 h, during which time a lot of precipitate was formed. The resulting solid was collected by filtration and washed with small amount of EtOAc and water to give the expected product as off-white solid (550 mg, yield: 82%). The resulting solid was dissolved in 3 mL of dimethylamine (2M in THF), and the mixture was heated at 130° C. for 40 min in microwave. The reaction mixture was then concentrated in vacuum, and the residue was treated with ethanol to give the product as off-white solid (520 mg, 93% yield); Mp: 194° C. MS (ESI) m/z 621.2.
To a solution of 1-benzyl-N-[4-({[2-(dimethylamino)-7-iodoquinazolin-4-yl]amino}methyl)phenyl]piperidine-4-carboxamide (200 mg, 0.32 mmol) in DMF (2 mL), was added PdCl2(PPh3)2 (11 mg, 5 mol %) as catalyst, followed by addition of tributyl(vinyl)tin (0.14 mL, 0.48 mmol). The resulting mixture was heated at 110° C. under nitrogen for 10 min in microwave. Upon completion, the reaction mixture was cooled down to RT, and then poured into cold water. The resulting solid was collected by filtration, and then purified by flash chromatography (CH2Cl2:CH3OH=90:10) to give off-white solid (129 mg, yield: 78%). Mp: 180° C.; MS (ESI) m/z 521.3.
This compound was prepared from 1-benzyl-N-[4-({[2-(dimethylamino)-7-iodoquinazolin-4-yl]amino}methyl)phenyl]piperidine-4-carboxamide (200 mg, 0.32 mmol) and (E)-tributyl(1-propenyl)tin (159 mg, 0.48 mmol) by following the same procedure as Example 150 (step 2) as yellow solid (163 mg, yield: 95%). mp 154° C.; HRMS: calcd for C33H38N6O+H+, 535.31799; found (ESI-FTMS, [M+H]1+), 535.31901.
This compound was prepared from 1-benzyl-N-[4-({[2-(dimethylamino)-7-iodoquinazolin-4-yl]amino}methyl)phenyl]piperidine-4-carboxamide (150 mg, 0.24 mmol) and (E)-3,3-dimethylbut-1-enylboronic acid (62 mg, 0.48 mmol) by following the same procedure as Example 97 (Suzuki coupling) as off-white solid (100 mg, yield: 72%). HRMS: calcd for C36H44N6O+H+, 577.36494; found (ESI-FTMS, [M+H]1+), 577.36625.
This compound was prepared from 1-benzyl-N-[4-({[2-(dimethylamino)-7-iodoquinazolin-4-yl]amino}methyl)phenyl]piperidine-4-carboxamide (150 mg, 0.24 mmol) and (Z)-1-propenylboronic acid (42 mg, 0.48 mmol) by following the same procedure as Example 97 (Suzuki coupling) as off-white solid (38 mg, yield: 30%). HRMS: calcd for C33H38N6O+H+, 535.31799; found (ESI-FTMS, [M+H]1+), 535.31893.
This compound was prepared from 3-(trifluoromethyl)aniline (10.0 g, 62 mmol) by following the same procedure as Example 86 to give the product as white solid (3.425 g, 21% yield for 5 steps). HRMS: calcd for C9H3Cl2F3N2+H+, 266.96981; found (ESI-FTMS, [M+H]1+), 266.97.
This compound was prepared from 2,4-dichloro-6-(trifluoromethyl)quinazoline (266 mg, 1 mmol) and N-[4-(aminomethyl)phenyl]-1-(4-fluorobenzyl)piperidine-4-carboxamide (341 mg, 1 mmol) by following the same procedure as Example 129 (step 1) to give the product as off-white solid (516 mg, yield: 80%); MS (ESI) m/z 572.3.
To a solution of dimethylamine (2M in THF, 1 mL) was added N-[4-({[2-chloro-6-(trifluoromethyl)quinazolin-4-yl]amino}methyl)phenyl]-1-(4-fluorobenzyl)piperidine-4-carboxamide (34 mg, 0.06 mmol). The resulting mixture was heated at 130° C. for 30 min in microwave. The mixture was cooled to RT, and subjected to HPLC separation to give the product as off-white solid (23.7 mg, 68% yield). MS (ESI) m/z 581.3.
This compound was prepared from N-[4-({[2-chloro-6-(trifluoromethyl)quinazolin-4-yl]amino}methyl)phenyl]-1-(4-fluorobenzyl)piperidine-4-carboxamide (34 mg, 0.06 mmol) and azetidine hydrochloride (28 mg, 0.3 mmol) by following the same procedure as Example 129 (step 2) as off-white solid (13.5 mg, 38% yield). MS (ESI) m/z 593.3.
This compound was prepared from N-[4-({[2-chloro-6-(trifluoromethyl)quinazolin-4-yl]amino}methyl)phenyl]-1-(4-fluorobenzyl)piperidine-4-carboxamide (34 mg, 0.06 mmol) and pyrrolidine (21 mg, 0.3 mmol) by following the same procedure as Example 129 (step 2) as off-white solid (25.3 mg, 70% yield). MS (ESI) m/z 607.3.
This compound was prepared from N-[4-({[2-chloro-6-(trifluoromethyl)quinazolin-4-yl]amino}methyl)phenyl]-1-(4-fluorobenzyl)piperidine-4-carboxamide (34 mg, 0.06 mmol) and 4-pyrimidin-2-ylpiperazine dihydrochloride (71 mg, 0.3 mmol) by following the same procedure as Example 129 (step 2) as off-white solid (6.1 mg, 15% yield). MS (ESI) m/z 700.3.
This compound was prepared from N-[4-({[2-chloro-6-(trifluoromethyl)quinazolin-4-yl]amino}methyl)phenyl]-1-(4-fluorobenzyl)piperidine-4-carboxamide (34 mg, 0.06 mmol) and diethylamine (22 mg, 0.3 mmol) by following the same procedure as Example 129 (step 2) as off-white solid (25.7 mg, 70% yield). MS (ESI) m/z 609.2.
This compound was prepared from N-[4-({[2-chloro-6-(trifluoromethyl)quinazolin-4-yl]amino}methyl)phenyl]-1-(4-fluorobenzyl)piperidine-4-carboxamide (34 mg, 0.06 mmol) and cyclobutylamine hydrochloride (32 mg, 0.3 mmol) by following the same procedure as Example 129 (step 2) as off-white solid (19.9 mg, 55% yield). MS (ESI) m/z 607.2.
This compound was prepared from N-[4-({[2-chloro-6-(trifluoromethyl)quinazolin-4-yl]amino}methyl)phenyl]-1-(4-fluorobenzyl)piperidine-4-carboxamide (34 mg, 0.06 mmol) and methylamine hydrochloride (20 mg, 0.3 mmol) by following the same procedure as Example 129 (step 2) as off-white solid (15.3 mg, 45% yield). MS (ESI) m/z 567.3.
Step 1 Synthesis of 4-chloro-N-[4-({[2-chloro-6-(trifluoromethyl)quinazolin-4-yl]amino}methyl)phenyl]benzamide. This compound was prepared from 2,4-dichloro-6-(trifluoromethyl)quinazoline (330 mg, 1.24 mmol) and N-[4-(aminomethyl)phenyl]-4-chlorobenzamide (322 mg, 1.24 mmol) by following the same procedure as Example 66 (step 1) to give the product as off-white solid (494 mg, yield: 81%); MS (ESI) m/z 491.1.
To a solution of dimethylamine (2M in THF, 1 mL) was added 4-chloro-N-[4-({[2-chloro-6-(trifluoromethyl)quinazolin-4-yl]amino}methyl)phenyl]benzamide (30 mg, 0.06 mmol). The resulting mixture was heated at 130° C. for 40 min in microwave. The mixture was cooled to RT, and subjected to HPLC separation to give the product as off-white solid (24.6 mg, 82% yield); MS (ESI) m/z 500.2.
This compound was prepared from 4-chloro-N-[4-({[2-chloro-6-(trifluoromethyl)quinazolin-4-yl]amino}methyl)phenyl]benzamide (30 mg, 0.06 mmol) and azetidine hydrochloride (28 mg, 0.3 mmol) by following the same procedure as Example 113 (step 2) as off-white solid (16.4 mg, 53% yield); MS (ESI) m/z 512.2.
This compound was prepared from 4-chloro-N-[4-({[2-chloro-6-(trifluoromethyl)quinazolin-4-yl]amino}methyl)phenyl]benzamide (30 mg, 0.06 mmol) and pyrrolidine (21 mg, 0.3 mmol) by following the same procedure as Example 113 (step 2) as off-white solid (21.3 mg, 67% yield). MS (ESI) m/z 526.2.
This compound was prepared from 4-chloro-N-[4-({[2-chloro-6-(trifluoromethyl)quinazolin-4-yl]amino}methyl)phenyl]benzamide (30 mg, 0.06 mmol) and 4-pyrimidin-2-ylpiperazine dihydrochloride (71 mg, 0.3 mmol) by following the same procedure as Example 113 (step 2) as off-white solid (3.2 mg, 9% yield). MS (ESI) m/z 619.2.
This compound was prepared from 4-chloro-N-[4-({[2-chloro-6-(trifluoromethyl)quinazolin-4-yl]amino}methyl)phenyl]benzamide (30 mg, 0.06 mmol) and diethylamine (22 mg, 0.3 mmol) by following the same procedure as Example 113 (step 2) as off-white solid (26 mg, 82% yield). MS (ESI) m/z 528.2.
This compound was prepared from 4-chloro-N-[4-({[2-chloro-6-(trifluoromethyl)quinazolin-4-yl]amino}methyl)phenyl]benzamide (30 mg, 0.06 mmol) and cyclobutylamine hydrochloride (32 mg, 0.3 mmol) by following the same procedure as Example 113 (step 2) as off-white solid (3.7 mg, 12% yield). MS (ESI) m/z 526.2.
This compound was prepared from 4-chloro-N-[4-({[2-chloro-6-(trifluoromethyl)quinazolin-4-yl]amino}methyl)phenyl]benzamide (30 mg, 0.06 mmol) and methylamine hydrochloride (20 mg, 0.3 mmol) by following the same procedure as Example 113 (step 2) as off-white solid (8.2 mg, 28% yield). MS (ESI) m/z 486.2.
This compound was prepared from 4-chloro-N-[4-({[2-chloro-6-(trifluoromethyl)quinazolin-4-yl]amino}methyl)phenyl]benzamide (30 mg, 0.06 mmol) and furfurylamine (29 mg, 0.3 mmol) by following the same procedure as Example 113 (step 2) as off-white solid (26 mg, 79% yield). MS (ESI) m/z 552.2.
N-(4-((2-dimethylamino)-6-(5-(dimethylamino)pyridine-2-yl)quinazolin-4-ylamino)methyl)phenyl)-4-fluorobenzamide was prepared starting from N-(4-((2-(dimethylamino)-6-iodoquinazolin-4-ylamino)methyl)phenyl)-4-fluorobenzide (0.15 g, 0.36 mmol), 6-(dimethylamino)pyridine-3-ylboronic acid (0.119 g, 0.71 mmol) tetrakis(triphenylphosphine)palladium (0) (50 mg, 0.0551 mmol), Toluene (8 mL), methanol (2 mL) and sodium carbonate (2.0 M solution) (4 mL). The reaction mixture was refluxed for 4 hours, and then cooled to room temperature. The mixture was partioned between chloroform (150 mL) and water (150 mL). The water layer was extracted twice with chloroform (150 mL). The combined organic layer was dried over magnesium sulfate. The residue was chromatographed on silica gel using ethyl acetate/MeOH (20:1) to yield the final product 112 mg (yield 58%). MS (ESI) m/z 536.1; MS (ESI) m/z 268.6.
4-((2-dimethylamino)-6-(3-(dimethylamino)phenyl)quinazolin-4-ylamino)methyl)N-4-fluorophenyl)benzamide was prepared starting from N-(4-((2-(dimethylamino)-6-iodoquinazolin-4-ylamino)methyl)phenyl)-4-fluorobenzamide (0.53 g, 0.98 mmol), 3-(dimethylamino)phenylboronic acid (0.16 g, 0.98 mmol) tetrakis(triphenylphosphine)palladium (0) (50 mg, 0.0551 mmol), Toluene (8 mL), methanol (2 mL), sodium carbonate (2.0 M solution) (4 mL). The reaction mixture was refluxed for 4 hours, and then cooled to room temperature. The mixture was partioned between chloroform (150 mL) and water (150 mL). The combined organic layer was dried with magnesium sulfate. The residue was chromatographed on silica gel using a ethyl acetate/MeOH (20:1) as an eluent to yield the final product 62 mg (yield 12%). MS (ESI) m/z 535.3.
Z-(4-((2-dimethylamino)-6-(styrylquinazolin-4-ylamino)-N-(4-fluorophenyl)benzamide was prepared starting from N-(4-((2-(dimethylamino)-6-iodoquinazolin-4-ylamino)methyl)phenyl)-4-fluorobenzamide, (0.36 g, 0.66 mmol), (Z)-styrylboronic acid (97 mg, 0.66 mmol) tetrakis(triphenylphosphine)palladium (0) (50 mg, 0.0551 mmol), Toluene (8 mL), methanol (2 mL), sodium carbonate (2.0 M solution) (4 mL). The reaction mixture was refluxed for 4 hours, and then cooled to room temperature. The mixture was partioned between chloroform (150 mL) and water (150 mL). The water layer was extracted twice with chlororm (150 mL). The combined organic layer was dried with magnesium sulfate. The residue was chromatographed on silica gel using a Hexanes/Ethyl acetate (1:1) as an eluent to yield the final product 62 mg (yield 15%). MS (ESI) m/z 518.3.
4-((2-dimethylamino)-6-(3-vinylphenyl)quinazolin-4-ylamino)methyl)-N-(4-fluorophenyl)benzamide was prepared starting from N-(4-((2-(dimethylamino)-6-iodoquinazolin-4-ylamino)methyl)phenyl)-4-fluorobenzamide (0.111 g, 0.20 mmol), tributyl(vinyl)stannane (65 mg, 0.2 mmol) PdCl2(PPh3)2 (50 mg, 0.071 mmol), DMF (20 mL). The reaction mixture was refluxed for 4 hours. The solvent was removed under reduced pressure and the residue partioned between chloroform (150 mL) and water (150 mL). The water layer was extracted twice with chloroform (150 mL). The combined organic layer was dried with magnesium sulfate. The residue was chromatographed on silica gel using CHCl3/MeOH (20:2) as an eluent to yield the final product 62 mg (yield 68%). MS (ESI) m/z 442.2.
E-(4-((2-dimethylamino)-6-(4-styrylphenyl)quinazolin-4-ylamino)methyl-N-(4-fluorophenyl)benzamide was prepared starting from N-(4-((2-(dimethylamino)-6-iodoquinazolin-4-ylamino)methyl)phenyl)-4-fluorobenzamide (0.41 g, 0.75 mmol), (E)-styrylboronic acid (111 mg, 0.75 mmol) tetrakis(triphenylphosphine)palladium (0) (50 mg, 0.0551 mmol), Toluene (8 mL), methanol (2 mL), sodium carbonate (2.0 M solution) (4 mL). The reaction mixture was refluxed for 4 hours, and then cooled to room temperature. The mixture was partioned between chloroform (150 mL) and water (150 mL). The combined organic layer was dried with magnesium sulfate. The residue was chromatographed on silica gel using Hexanes/Ethyl acetate (1:1) as an eluent to yield the final product 40 mg (yield 10%). MS (ESI) m/z 518.3.
E-4-((2-dimethylamino)-6-(prop-1-enyl)quinazolin-4-ylamino)methyl-N-(4-fluorophenyl)benzamide was prepared starting from N-(4-((2-(dimethylamino)-6-iodoquinazolin-4-ylamino)methyl)phenyl)-4-fluorobenzamide (0.5 g, 0.92 mmol), (E)-tributyl(prop-1-enyl)stannane (0.30 g, 0.90 mmol) PdCl2(PPh3)2 (50 mg, 0.071 mmol), DMF (20 mL). The reaction mixture was refluxed for 4 hours, and then cooled to room temperature. The mixture was partioned between chloroform (150 mL) and water (150 mL). The water layer was extracted twice with chlororm (150 mL). The combined organic layer was dried with magnesium sulfate. The residue was chromatographed on silica gel using 50% CH2Cl2/EtOAc and 5% MeOH as en eluent to give 100 mg (24% Yield) of the final product. MS (ESI) m/z 456.3.
E-(4-((2-dimethylamino)-6-(hex-1-enyl)quinazolin-4-ylamino)methyl)-N-(4-fluorophenyl)benzamide was prepared starting from N-(4-((2-(dimethylamino)-6-iodoquinazolin-4-ylamino)methyl)phenyl)-4-fluorobenzamide (0.50 g, 0.92 mmol), trans-1-hexen-boronic acid (120 mg, 0.93 mmol) (tetrakis(triphenylphosphine)palladium (0) (50 mg, 0.0551 mmol), Toluene (8 mL), methanol (2 mL), sodium carbonate (2.0 M solution) (4 mL). The reaction mixture was refluxed for 4 hours, and then cooled to room temperature. The mixture was partioned between chloroform (150 mL) and water (150 mL). The water layer was extracted twice with chlororm (150 mL). The combined organic layer was dried with magnesium sulfate. The residue was chromatographed on silica gel using Hexanes/Ethyl acetate (1:1) as an eluent to yield the final product 80 mg (63% yield). MS (ESI) m/z 498.4.
(E)-N-{4-[({2-(dimethylamino)-6-(3,3-dimethylbut-1-enyl)quinazolin-4-ylamino)methyl)-N-(4-fluorophenyl)benzamide, E-(4-((2-dimethylamino)-6-(4-styrylphenyl)quinazolin-4-ylamino)methyl-N-(4-fluorophenyl)benzamide was prepared starting from N-(4-((2-(dimethylamino)-6-iodoquinazolin-4-ylamino)methyl)phenyl)-4-fluorobenzamide (0.50 g, 0.92 mmol), (E)-3,3-dimethylbut-1-enylboronic acid (118 mg, 0.92 mmol) (tetrakis(triphenylphosphine)palladium (0) (50 mg, 0.0551 mmol), Toluene (8 mL), methanol (2 mL), sodium carbonate (2.0 M solution) (4 mL). The reaction mixture was refluxed for 4 hours, and then cooled to room temperature. The mixture was partioned between chloroform (150 mL) and water (150 mL). The water layer was extracted twice with chlororm (150 mL). The combined organic layer was dried with magnesium sulfate. The residue was chromatographed on silica gel using a eluente Hexanes/Ethyl acetate (1:1) to yield the final product 50 mg (yield 11%). MS (ESI) m/z 498.4.
A mixture of 4-isopropylaniline (3.76, 27.85 mol) and ethoxycarbonyl thioisocyanate (3.65 g, 27.85 mmol) was stirred in dichlormethane at room temperature for 3 hours. The separated solid was filtered and washed with hexanes, to give 5.17 g (70% yield) of the product. MS (ESI) m/z 267.2.
A mixture of ethyl {[(4-isopropylphenyl)amino]carbonothioyl}carbamate) (5.17 g, 17.58 mmol), dry acetone 300 (mL), potassium carbonate (13.41 g, 97.17 mmol) was stirred for five minutes at room temperature. To the reaction mixture was added ethyliodide (3.15 g, 20.19 mmol) and stirred at room temperature for 24 hours. The reaction mixture was filtered and the solvent was removed. The residue was extracted with dichloromethane (200 mL) and washed with water. The organic layer was dried over magnesium sulfate; filtered and concentrated to give 4.5 g (93%) of the product. MS (ESI) m/z 295.2.
A mixture of ethyl {(E)-(ethylthio)[(4-isopropylphenyl)imino]methyl}carbamate (4.5 g, 15.30 mmol), diphenylether 40 (mL) was heated to 235° C. for 2 hours. The mixture was cooled at room temperature and the separated solid was filtered and washed with plenty of hexanes. The residue was dried at room temperature to give 2.67 g (70% yield) of the product. MS (ESI) m/z 249.3.
A mixture of 2-isopropyaniline (3.76 g, 27.85 mmol) and ethoxycarbonyl thioisocyanate (3.65 g, 27.85 mmol), was stirred in dichloromethane at room temperature for 3 hours. At the end, reaction mixture was concentrated and the separated solid was washed with hexanes and dried to give 5.17 g (70% yield) of the product. MS (ESI) m/z 267.2.
A mixture of ethyl {[(2-isopropylphenyl)amino]carbonothioyl}carbamate) (10 g, 35.16 mmol), dry acetone 500 (mL), potassium carbonate (26.82 g, 0.194 mol) was stirred for five minutes at room temperature. To the reaction mixture was added ethyliodide (6.3 g, 40.38 mmol) then stirred at room temperature for 24 hours. The reaction mixture was filtered and the solvent was removed. The residue was extracted with dichloromethane (200 mL) and washed with water. The organic layer was dried over magnesium sulfate; filtered and concentrated to give 9.0 g (93%) of the product. MS (ESI) m/z 295.2.
A mixture of ethyl {(E)-(ethylthio)[(2-isopropylphenyl)imino]methyl}carbamate) (9.0 g, 30.6 mmol), diphenylether 40 (mL) was heated to 235° C. for 2 hours. The mixture was cooled at room temperature and the separated solid was washed with plenty of hexanes. The residue was dried at room temperature to give 6.8 g (89% yield) of the 2-(ethylthio)-8-isopropylquinazolin-4(3H)-one. MS (ESI) m/z 249.3.
A mixture of 3-fluoro-2-methyl-aniline (10.0 g, 80.0 mmol), ethoxycarbonyl thioisocyanate (10.48 g, 80.0 mmol), dichloromethane (400 mL) was stirred at room temperature for 3 hours. At the end, reaction mixture was concentrated and the separated solid was washed with hexanes, dried to give ethyl {[(3-fluoro-2-methylphenyl)amino]carbonothioyl}carbamate 15.0 g (76% yield). MS (ESI) m/z 257.1.
A mixture of ethyl {[(3-fluoro-2-methylphenyl)amino]carbonothioyl}carbamate) (10.0 g, 39.06 mmol), dry acetone 350 (mL), potassium carbonate (13.41 g, 97.17 mmol) was stirred for five minutes at room temperature. To the reaction mixture was added ethyliodide (6.34 g, 40.0 mmol) then stirred at room temperature for 24 hours. At the end, reaction mixture was filtered and concentrated to give 10.81 g (97%) of the product. MS (ESI) m/z 285.1.
A mixture of ethyl {(ethylthio)[(3-fluoro-2-methylphenyl)amino]methyl}carbamate) (10 g, 34.97 mmol), diphenylether 40 (mL) was heated to 235° C. for 2 hours. The mixture was cooled at room temperature and the separated solid was filtered and washed with plenty of hexanes. The residue was dried at room temperature to give 7.0 g (84% yield) of the product. MS (ESI) m/z 239.1.
A mixture of 2-(ethylthio)-8-isopropylquinazolin-4(3H)-one) (3.8 g, 15.32 mmol), HCl 6N (150 mL), Ethanol (150 mL) was refluxed for 24 hours. The solvent was removed and the mixture was adjusted to pH 6.0. by using NH4OH solution. The precipitate was filtered, dried to give 3.0 g (95%) of the product. MS (ESI) m/z 203.
A mixture of 3-isopropylaniline (5.15 g, 38.19 mmol) and ethoxycarbonyl thioisocyanate (5.0 g, 38.16 mmol) was stirred in dichloromethane (250 mL) at room temperature for 3 hours. The reaction mixture was concentrated and the separated solid was washed with hexanes, dried to give (ethyl {[(3-isopropylphenyl)amino]carbonothioyl}carbamate; 7.8 g (78% yield). MS (ESI) m/z 267.4.
A mixture of ethyl {[(3-isopropylphenyl)amino]carbonothioyl}carbamate) (5.17 g, 17.58 mmol), dry acetone 300 (mL), potassium carbonate (13.41 g, 97.17 mmol) was stirred for five minutes at room temperature. To the reaction mixture was added ethyliodide (3.15 g, 20.19 mmol) and then stirred at room temperature for 24 hours.
The reaction mixture was filtered and concentrated. The residue was combined with water 200 (mL) and extracted with dichloromethane (200 mL) and dried over magnesium sulfate to give 4.5 g (93%) of the product. MS (ESI) m/z 295.1.
A mixture of ethyl {(ethylthio)[(3-isopropylphenyl)amino]methyl}carbamate (4.5 g, 15.30 mmol), diphenylether 40 (mL) was heated to 235° C. for 2 hours. The mixture was cooled to room temperature and the solid was washed with plenty of hexanes. The residue was dried at room temperature to give 2.67 g (70% yield) of the product 2-(ethylthio)-7-isopropylquinazolin-4(3H)-one was isolated as brown solid. MS (ESI) m/z 249.2.
A mixture of 4-bromophenyl isocyanate (5.0 g, 25.22 mmol) in dichloromethane (400 mL), (4-amino-benzyl)-carbamic acid tert-butyl ester (5.6 g, 25.2 mmol) was stirred at room temperature for 24 hours. At the end, reaction mixture was concentrated and the residue was triturated with diethylether and the precipitate was filtered. The crude solid was taken up in TFA 100 (mL) and stirred at room temperature overnight. The mixture was poured over ice cold water and the pH was adjusted to 10, using 10 N. sodium hydroxide (NaOH). The product was extracted with chloroform and concentrated to give 2.0 g (24% yield) of the product. MS (ESI) m/z 322.
A mixture of 4-fluorophenyl isocyanate (5.0 g, 36.5 mmol) in dichloromethane (400 mL), (4-amino-benzyl)-carbamic acid tert-butyl ester (8.10 g, 36.5 mmol) was stirred at room temperature for 24 hours. At the end, reaction mixture was concentrated and the residue was triturated with diethylether and the precipitate was filtered. The crude solid was taken up in TFA 100 (mL) and stirred at room temperature overnight. The mixture was poured over ice cold water and the pH was adjusted to 10, using 10 N. NaOH. The product was extracted with chloroform and concentrated to give 2.4 g (25% yield) of the product. MS (ESI) m/z 258.1.
A mixture of 4-chlorophenyl isocyanate (5.0 g, 32.6 mmol) in dichloromethane (400 mL), (4-amino-benzyl)-carbamic acid tert-butyl ester (7.2 g, 32.6 mmol) was stirred at room temperature for 24 hours. At the end, reaction mixture was concentrated and the residue was triturated with diethylether and the precipitate was filtered. The crude solid was taken up in TFA 100 (mL) and stirred at room temperature overnight. The mixture was poured over ice cold water and the pH was adjusted to 10, using 10 N. NaOH. The product was extracted with chloroform and concentrated to give 3.1 g (34% yield) of the product. MS (ESI) m/z 276.5.
Step 1: To a stirred solution of 7-methyl-2,4-dichloroquinazoline (800 mg, 3.7 mmol) and N-[4-(aminomethyl)phenyl]-N′-(4-chlorophenyl)urea (1030 mg, 3.7 mmol) in DMF (25 mL) Et3N (15 mL) was added at room temperature and continued for 5 h. The reaction mixture was concentrated and water (100 mL) was added. Separated solid was filtered and washed with water. The separated solid was suspended in diethylether and filtered. Yield: 1300 mg, 77%.
Step 2: A mixture of N-(4-chlorophenyl)-N′-[4-({[2-(chloro)-7-methylquinazolin-4-yl]amino}methyl)phenyl]urea) (1000 mg, 2.2 mmol) and dimethylamine hydrochloride (20 g) in THF/Isopropanol (1:1) 500 (mL) was refluxed for 2 hours, and then cooled to room temperature. Half of solvent volume was evaporated and the mixture was partioned between chloroform (200 mL) and water (200 mL). The water layer was extracted three with chlororm (200 mL). The combined organic layer was dried with magnesium sulfate, filtered. The solvent was evaporated and the residue was washed with plenty of ethyl acetate to give 330 mg (33%) of the product was isolated. MS (ESI) m/z 461.2.
Step 1: To a stirred solution of 6-methyl-2,4-dichloroquinazoline (390 mg, 1.83 mmol) and N-[4-(aminomethyl)phenyl]-N′-(4-chlorophenyl)urea (470 mg, 1.81 mmol) in DMF (25 mL) Et3N (7 mL) was added at room temperature and continued for 5 h. The reaction mixture was concentrated and water (100 mL) was added. Separated solid was filtered and washed with water. The separated solid was suspended in diethylether and filtered. Yield: 300 mg, 46%.
Step 2: A mixture of N-(4-chlorophenyl)-N′-[4-({[2-(chloro)-6-methylquinazolin-4-yl]amino}methyl)phenyl]urea) (800 mg, 1.7 mmol) and dimethylamine hydrochloride (10 g) in THF/Isopropanol (1:1) 500 (mL) was refluxed for 2 hours, and then cooled to room temperature. Half of solvent volume was evaporated and the mixture was partioned between chloroform (200 mL) and water (200 mL). The water layer was extracted three with chlororm (200 mL). The combined organic layer was dried with magnesium sulfate, filtered. The solvent was evaporated and the residue was washed with plenty of ethyl acetate to give 384 mg (47%) of the product was isolated. MS (ESI) m/z 461.2.
Step 1: To a stirred solution of 7-methyl-2,4-dichloroquinazoline (300 mg, 1.40 mmol) and N-[4-(aminomethyl)phenyl]-N′-(4-bromophenyl)urea (450 mg, 1.4 mmol) in DMF (25 mL) Et3N (7 mL) was added at room temperature and continued for 5 h. The reaction mixture was concentrated and water (100 mL) was added. Separated solid was filtered and washed with water. The separated solid was suspended in diethylether and filtered. Yield: 200 mg, 28%.
Step 2: A mixture of N-(4-bromophenyl)-N′-[4-({[2-(chloro)-7-methylquinazolin-4-yl]amino}methyl)phenyl]urea) (500 mg, 1 mmol) and dimethylamine hydrochloride (10 g) in THF/Isopropanol (1:1) 500 (mL) was refluxed for 2 hours, and then cooled to room temperature. Half of solvent volume was evaporated and the mixture was partioned between chloroform (200 mL) and water (200 mL). The water layer was extracted three with chlororm (200 mL). The combined organic layer was dried with magnesium sulfate, filtered. The solvent was evaporated and the residue was washed with plenty of ethyl acetate to give 100 mg (20%) of the product was isolated. MS (ESI) m/z 506.3.
Step 1: To a stirred solution of 7-methyl-2,4-dichloroquinazoline (1230 mg, 5.8 mmol) and N-[4-(aminomethyl)phenyl]-N′-(4-fluorophenyl)urea (1500 mg, 5.8 mmol) in DMF (30 mL) Et3N (10 mL) was added at room temperature and continued for 12 h. The reaction mixture was concentrated and water (100 mL) was added. Separated solid was filtered and washed with water. The separated solid was suspended in diethylether and filtered. Yield: 1100 mg, 44%.
Step 2: A mixture of N-(4-fluorophenyl)-N′-[4-({[2-(chloro)-7-methylquinazolin-4-yl]amino}methyl)phenyl]urea) (1000 mg, 2.3 mmol) and dimethylamine hydrochloride (16 g) in THF/Isopropanol (1:1) 500 (mL) was refluxed for 5 hours, and then cooled to room temperature. Half of solvent volume was evaporated and the mixture was partioned between chloroform (200 mL) and water (200 mL). The water layer was extracted three with chlororm (200 mL). The combined organic layer was dried with magnesium sulfate, filtered. The solvent was evaporated and the residue was washed with plenty of ethyl acetate to give 100 mg (20%) of the product was isolated. MS (ESI) m/z 445.3.
Step 1: To a stirred solution of 6-methyl-2,4-dichloroquinazoline (400 mg, 1.8 mmol) and N-[4-(aminomethyl)phenyl]-N′-(4-fluorophenyl)urea (480 mg, 1.8 mmol) in DMF (20 mL) Et3N (10 mL) was added at room temperature and continued for 5 h. The reaction mixture was concentrated and water (100 mL) was added. Separated solid was filtered and washed with water. The separated solid was suspended in diethylether and filtered. Yield: 300 mg, 37%.
Step 2: A mixture of N-(4-fluorophenyl)-N′-[4-({[2-(chloro)-6-methylquinazolin-4-yl]amino}methyl)phenyl]urea) (300 mg, 0.69 mmol) and dimethylamine hydrochloride (10 g) in THF/Isopropanol (1:1) 500 (mL) was refluxed for 12 hours, and then cooled to room temperature. Half of solvent volume was evaporated and the mixture was partioned between chloroform (200 mL) and water (200 mL). The water layer was extracted three with chlororm (200 mL). The combined organic layer was dried with magnesium sulfate, filtered. The solvent was evaporated and the residue was washed with plenty of ethyl acetate to give 150 mg (50%) of the product was isolated. MS (ESI) m/z 445.3.
Step 1: To a stirred solution of 2,4-dichloroquinazoline (710 mg, 3.6 mmol) and N-[4-(aminomethyl)phenyl]-N′-(4-fluorophenyl)urea (920 mg, 3.6 mmol) in DMF (20 mL) Et3N (10 mL) was added at room temperature and continued for 5 h. The reaction mixture was concentrated and water (100 mL) was added. Separated solid was filtered and washed with water. The separated solid was suspended in diethylether and filtered. Yield: 1000 mg, 66%.
Step 2: A mixture of N-(4-fluorophenyl)-N′-[4-({[2-(chloro)-quinazolin-4-yl]amino}methyl)phenyl]urea) (1000 mg, 2.4 mmol) and dimethylamine hydrochloride (15 g) in THF/Isopropanol (1:1) 500 (mL) was refluxed for 72 hours, and then cooled to room temperature. Half of solvent volume was evaporated and the mixture was partioned between chloroform (200 mL) and water (200 mL). The water layer was extracted three with chlororm (200 mL). The combined organic layer was dried with magnesium sulfate, filtered. The solvent was evaporated and the residue was washed with plenty of ethyl acetate to give 80 mg (8%) of the product was isolated. MS (ESI) m/z 431.3.
6-chloro-N-[4-({[8-methyl-2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]nicotinamide was prepared starting from 8-methyl-2,4-dichloroquinazoline, 4-aminobenzylamine and 6-chloronicotinoylchloride by following the procedure A (step 1). The intermediate product from the step 1 was aminated using monomethylamine to yield the final product. Starting from (544 mg, 2.5 mmol) of 8-methyl-2,4-dichloroquinazoline, 250 mg (Yield, 23%) of the final product was isolated. MS (EST) m/z 433.2. During this reaction 14% of 6-(methylamino)-N-[4-({[8-methyl-2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]nicotinamide was also isolated. MS (ESI)/Z 428.3.
6-chloro-N-[4-({[2-(methylamino)-6-nitroquinazolin-4-yl]amino}methyl)phenyl]nicotinamide was prepared starting from 6-nitro-2,4-dichloroquinazoline, 4-aminobenzylamine and 6-chloronicotinoylchloride by following the procedure A (step 1). The intermediate product from the step 1 was aminated using monomethylamine to yield the final product. Starting from (500 mg, 2.05 mmol) of 6-nitro-2,4-dichloroquinazoline, (400 mg, Yield, 42%) of the final product was isolated. MS (ESI) m/z 464.1; mp 305-307° C.
6-chloro-N-[4-({[2-(methylamino)-8-nitroquinazolin-4-yl]amino}methyl)phenyl]nicotinamide was prepared starting from 8-nitro-2,4-dichloroquinazoline, 4-aminobenzylamine and 6-chloronicotinoylchloride by following the procedure A (step 1). The intermediate product from the step 1 was aminated using monomethylamine to yield the final product. Starting from (1000 mg, 4.14 mmol) of 8-nitro-2,4-dichloroquinazoline, (400 mg, Yield, 45%) of the final product was isolated. MS (ESI) m/z 464.1.
4-fluoro-N-[4-({[2-(methylamino)-6-nitroquinazolin-4-yl]amino}methyl)phenyl]benzamide was prepared starting from 6-nitro-2,4-dichloroquinazoline, 4-aminobenzylamine and 4-fluorobenzoyl chloride by following the procedure A (step 1). The intermediate product from the step 1 was aminated using monomethylamine to yield the final product. Starting from (1000 mg, 4.12 mmol) of 6-nitro-2,4-dichloroquinazoline, 210 mg (Yield, 20%) of the final product was isolated. MS (ESI) m/z 447.3.
N-[4-({[2-(dimethylamino)-6-nitroquinazolin-4-yl]amino}methyl)phenyl]-4-fluorobenzamide was prepared starting from 6-nitro-2,4-dichloroquinazoline, 4-aminobenzylamine and 4-fluorobenzoyl chloride by following the procedure A (step 1). The intermediate product from the step 1 was aminated using monomethylamine to yield the final product. Starting from (1000 mg, 4.12 mmol) of 6-nitro-2,4-dichloroquinazoline, 400 mg (Yield, 22%) of the final product was isolated. MS (ESI) m/z 461.3.
N-[4-({[6-nitro-2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]nicotinamide was prepared starting from 6-nitro-2,4-dichloroquinazoline, 4-aminobenzylamine and nicotinoylchloride by following the procedure A (step 1). The intermediate product from the step 1 was aminated using monomethylamine to yield the final product. Starting from (2 g, 8.23 mmol) of 6-nitro-2,4-dichloroquinazoline, (630 mg, Yield, 18%) of the final product was isolated. MS (ESI) m/z 400.1. mp 98-106° C.
3,4-difluoro-N-[4-({[5-methyl-2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]benzamide was prepared starting from 5-methyl-2,4-dichloroquinazoline, 4-aminobenzylamine and 3,4-difluorobenzoylchloride by following the procedure A (step 1). The intermediate product from the step 1 was aminated using monomethylamine to yield the final product. Starting from (1000 mg, 4.69 mmol) of 5-methyl-2,4-dichloroquinazoline, 20 mg (Yield, 1%) of the final product was isolated. MS (ESI) m/z 434.3.
4-fluoro-N-[4-({[8-methyl-2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]benzamide was prepared starting from 8-methyl-2,4-dichloroquinazoline, 4-aminobenzylamine and 4-fluorobenzoylchloride by following the procedure A (step 1). The intermediate product from the step 1 was aminated using monomethylamine to yield the final product. Starting from (500 mg, 2.35 mmol) of 8-methyl-2,4-dichloroquinazoline, 274 mg (Yield, 28%) of the final product was isolated. MS (ESI) m/z 416.1.
4-chloro-N-[4-({[8-methyl-2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]benzamide was prepared starting from 8-methyl-2,4-dichloroquinazoline, 4-aminobenzylamine and 4-chlorobenzoylchloride by following the procedure A (step 1). The intermediate product from the step 1 was aminated using monomethylamine to yield the final product. Starting from (500 mg, 2.35 mmol) of 8-methyl-2,4-dichloroquinazoline, 320 mg (Yield, 32%) of the final product was isolated. MS (ESI) m/z 432.1.
4-fluoro-N-[4-({[5-methyl-2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]benzamide was prepared starting from 5-methyl-2,4-dichloroquinazoline (426 mg, 2 mmol), 4-aminobenzylamine and 4-fluorobenzoylchloride by following the procedure A (step 1) to give the 4-fluoro-N-[4-({[2-chloro-5-methyl-quinazolin-4-yl]amino}methyl)phenyl]benzamide (490 mg, yield 58%). The product (250 mg, 0.6 mmol) from the step 1 was aminated with monomethylamine to obtain the final product (98 mg, yield, 40%). MS (ESI) m/z 416.3; mp 228-230° C.
4-chloro-N-[4-({[5-methyl-2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]benzamide was prepared starting from 5-methyl-2,4-dichloroquinazoline (426 mg, 2 mmol), 4-aminobenzylamine and 4-chlorobenzoylchloride by following the procedure A (step 1) to give the 4-chloro-N-[4-({[2-chloro-5-methyl-quinazolin-4-yl]amino}methyl)phenyl]benzamide (155 mg, yield 18%). The product (120 mg, 0.28 mmol) from the step 1 was aminated with monomethylamine to obtain the final product (71 mg, yield, 60%). MS (ESI) m/z 432.3.
6-chloro-N-[4-({[5-methyl-2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]nicotinamide was prepared starting from 8-methyl-2,4-dichloroquinazoline (639 mg, 3.0 mmol), 4-aminobenzylamine and 6-chloronicotinoyl chloride by following the procedure A (step 1) to give the 6-chloro-N-[4-({[2-chloro-8-methyl-quinazolin-4-yl]amino}methyl)phenyl]nicotinamide (490 mg, yield 37%). The product (200 mg, 0.46 mmol) from the step 1 was aminated with monomethylamine hydrochloride to obtain the final product (66 mg, yield, 33%). MS (ESI) m/z 432.3; mp 233-236° C.
4-chloro-N-[4-({[7-methyl-2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]benzamide was prepared starting from 7-methyl-2,4-dichloroquinazoline (500 mg, 3.0 mmol), 4-aminobenzylamine and 4-chlorobenzoyl chloride by following the procedure A (step 1) to give the 4-chloro-N-[4-({[2-chloro-7-methyl-quinazolin-4-yl]amino}methyl)phenyl]benzamide (845 mg, yield 82%). The product (200 mg, 0.46 mmol) from the step 1 was aminated with monomethylamine hydrochloride to obtain the final product (150 mg, yield, 35%). MS (ESI) m/z 432.2 mp 264-266° C.
4-chloro-N-[4-({[2-(dimethylamino)-7-methyl-quinazolin-4-yl]amino}methyl)phenyl]benzamide was prepared starting from 7-methyl-2,4-dichloroquinazoline (500 mg, 3.0 mmol), 4-aminobenzylamine and 4-chlorobenzoyl chloride by following the procedure A (step 1) to give the 4-chloro-N-[4-({[2-chloro-7-methyl-quinazolin-4-yl]amino}methyl)phenyl]benzamide (845 mg, yield 82%). The product (150 mg, 0.35 mmol) from the step 1 was aminated with dimethylamine to obtain the final product (82 mg, yield, 54%). MS (ESI) m/z 446.1.
4-chloro-N-{4-[({7-methyl-2-[(2-pyridin-2-ylethyl)amino]quinazolin-4-yl}amino)methyl]phenyl}benzamide was prepared starting from 7-methyl-2,4-dichloroquinazoline (500 mg, 3.0 mmol), 4-aminobenzylamine and 4-chlorobenzoyl chloride by following the procedure A (step 1) to give the 4-chloro-N-[4-({[2-chloro-7-methyl-quinazolin-4-yl]amino}methyl)phenyl]benzamide (845 mg, yield 82%). The product (150 mg, 0.35 mmol) from the step 1 was aminated with 2-ethylamino-2-pyridine to obtain the final product (95 mg, yield, 53%). MS (ESI) m/z 523.1.
N-(4-{[(2-azepan-1-yl-7-methylquinazolin-4-yl)amino]methyl}phenyl)-4-chlorobenzamide was prepared starting from 7-methyl-2,4-dichloroquinazoline (500 mg, 3.0 mmol), 4-aminobenzylamine and 4-chlorobenzoyl chloride by following the procedure A (step 1) to give the 4-chloro-N-[4-({[2-chloro-7-methyl-quinazolin-4-yl]amino}methyl)phenyl]benzamide (845 mg, yield 82%). The product (156 mg, 0.36 mmol) from the step 1 was aminated with aza-cycloheptane to obtain the final product (124 mg, yield, 70%). MS (ESI) m/z 500.3; mp 192-194° C.
To a stirred suspension of N-[4-(aminomethyl)phenyl]-4-fluorobenzamide (1.40 g, 5.73 mmol) and triethylamine (2 mL) in THF (20 mL) at rt was added 7-methyl-2,4-dichloroquinazoline (1.22 g, 5.73 mmol) dissolved in CHCl3 (10 mL) and the mixture was kept stirring for a minimum of 3 hours. After TLC showed the complete disappearance of the 7-methyl-2,4-dichloroquinazoline, CHCl3 (250 mL) and water (25 mL) was added. The layers were separated, the aqueous layer was extracted twice with CHCl3 (25 mL), and the combined organic layers were dried over MgSO4. After removal of MgSO4 by filtration and evaporation of solvents the crude product was purified by column chromatography with hexane/CH2Cl2/TEA to give N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (1.80 g, 73% yield). MS (ESI) m/z 421.2.
N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (1 mmol) obtained by the procedure B, (step 1) was taken up either in a sealed tube or in round bottom flask and was suspended in and appropriate solvent THF (5 mL) or (dioxane, DMF, 2-propanol etc.) (5 mL). (If the reactant amine was mono methylamine or dimethylamine sealed tube was used and for other amines round bottom flask can be used.) The appropriate amine or amine hydrochloride was added and the mixture was heated under stirring over 2-16 h to 100-120° C. After the reaction was completed, the solvents were removed in vacuum and the crude compound was purified by preparative HPLC (high pressure liquid chromatography) using acetonitrile (ACN)/water/(NH3 or TFA)-gradients as eluent or column chromatography with CH2Cl2/MeOH/NH3 to give the diamino quinazolines in yields between 65-95%.
N-[4-({[2-(dimethylamino)-7-methylquinazolin-4-yl]amino}methyl)phenyl]-4-fluorobenzamide was prepared by amination of N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (150 mg, 0.36 mmol) with 2M dimethylamine hydrochloride in 2-propanol following the procedure B (step 2). After purification by column chromatography and solvent removal the final product (110 mg, yield, 71%) was isolated. MS (ESI) m/z 430.3.
4-fluoro-N-[4-({[7-methyl-2-(4-pyridin-2-ylpiperazin-1-yl)quinazolin-4-yl]amino}methyl)phenyl]benzamide was prepared by amination of N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (42 mg, 0.1 mmol) with 1-(2-pyridyl)-piperazine hydrochloride and NEt3 in dioxan following the procedure B (step 2). After purification by HPLC and solvent removal the final product (22 mg, yield, 65%) was isolated. MS (ESI) m/z 548.2.
N-{4-[({2-[[3-(dimethylamino)propyl](methyl)amino]-7-methylquinazolin-4-yl}amino)methyl]phenyl}-4-fluorobenzamide was prepared by amination of N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (42 mg, 0.1 mmol) with N,N,N′-trimethyl popyldiamine in dioxan following the procedure B (step 2). After purification by HPLC and solvent removal the final product (20 mg, yield, 66%) was isolated. MS (ESI) m/z 501.6.
N-(4-{[(2-azetidin-1-yl-7-methylquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide was prepared by amination of N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (42 mg, 0.1 mmol) with azetidine hydrochloride and NEt3 in dioxan following the procedure B (step 2). After purification by HPLC and solvent removal the final product (20 mg, yield, 74%) was isolated. MS (ESI) m/z 442.2.
N-{4-[({2-[benzyl(methyl)amino]-7-methylquinazolin-4-yl}amino)methyl]phenyl}-4-fluorobenzamide was prepared by amination of N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (42 mg, 0.1 mmol) with N-benzylmethylamine in dioxan following the procedure B (step 2). After purification by HPLC and solvent removal the final product (17 mg, yield, 54%) was isolated. MS (ESI) m/z 506.6.
4-fluoro-N-[4-({[7-methyl-2-(4-methylpiperazin-1-yl)quinazolin-4-yl]amino}methyl)phenyl]benzamide was prepared by amination of N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (42 mg, 0.1 mmol) 1-methyl piperazine in dioxan following the procedure B (step 2). After purification by HPLC and solvent removal the final product (20 mg, yield, 67%) was isolated. MS (ESI) m/z 485.6.
4-fluoro-N-{4-[({2-[(2S)-2-(methoxymethyl)pyrrolidin-1-yl]-7-methylquinazolin-4-yl}amino)methyl]phenyl}benzamide was prepared by amination of N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (42 mg, 0.1 mmol) 2-(S)-methoxymethylpyrrolidine in dioxan following the procedure B (step 2). After purification by HPLC and solvent removal the final product (27 mg, yield, 89%) was isolated. MS (ESI) m/z 500.6.
4-fluoro-N-(4-{[(7-methyl-2-piperidin-1-ylquinazolin-4-yl)amino]methyl}phenyl)benzamide was prepared by amination of N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (42 mg, 0.1 mmol) and piperidine in dioxan following the procedure B (step 2). After purification by HPLC and solvent removal the final product (11 mg, yield, 38%) was isolated. MS (ESI) m/z 470.6.
4-fluoro-N-(4-{[(7-methyl-2-morpholin-4-ylquinazolin-4-yl)amino]methyl}phenyl)benzamide was prepared by amination of N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (42 mg, 0.1 mmol) morpholine in dioxan following the procedure B (step 2). After purification by HPLC and solvent removal the final product (17 mg, yield, 60%) was isolated. MS (ESI) m/z 472.5.
4-fluoro-N-(4-{[(7-methyl-2-piperazin-1-ylquinazolin-4-yl)amino]methyl}phenyl)benzamide was prepared by amination of N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (42 mg, 0.1 mmol) piperazine in dioxan following the procedure B (step 2). After purification by HPLC and solvent removal the final product (21 mg, yield, 72%) was isolated. MS (ESI) m/z 471.6.
4-fluoro-N-(4-{[(7-methyl-2-pyrrolidin-1-ylquinazolin-4-yl)amino]methyl}phenyl)benzamide was prepared by amination of N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (42 mg, 0.1 mmol) pyrrolidine in dioxan following the procedure B (step 2). After purification by HPLC and solvent removal the final product (16 mg, yield, 56%) was isolated. MS (ESI) m/z 456.5.
N-{4-[({2-[ethyl(methyl)amino]-7-methylquinazolin-4-yl}amino)methyl]phenyl}-4-fluorobenzamide was prepared by amination of N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (42 mg, 0.1 mmol) N-ethylmethylamine in dioxan following the procedure B (step 2). After purification by HPLC and solvent removal the final product (23 mg, yield, 85%) was isolated. MS (ESI) m/z 444.5.
N-[4-({[2-(diethylamino)-7-methylquinazolin-4-yl]amino}methyl)phenyl]-4-fluorobenzamide was prepared by amination of N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (42 mg, 0.1 mmol) diethylamine in dioxan following the procedure B (step 2). After purification by HPLC and solvent removal the final product (18 mg, yield, 64%) was isolated. MS (ESI) m/z 458.6.
4-fluoro-N-[4-({[7-methyl-2-(4-phenylpiperazin-1-yl)quinazolin-4-yl]amino}methyl)phenyl]benzamide was prepared by amination of N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (30 mg, 0.07 mmol) 1-phenylpiperazine in dioxan following the procedure B (step 2). After purification by HPLC and solvent removal the final product (19 mg, yield, 40%) was isolated as bis-TFA salt. MS (ESI) m/z 547.7.
4-fluoro-N-{4-[({7-methyl-2-[4-(2-oxo-2-pyrrolidin-1-ylethyl)piperazin 1-yl]quinazolin-4-yl}amino)methyl]phenyl}benzamide was prepared by amination of N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (30 mg, 0.07 mmol) (1-pyrrolidinecarbonylmethyl)piperazine in dioxan following the procedure B (step 2). After purification by HPLC and solvent removal the final product (38 mg, yield, 78%) was isolated as bis-TFA salt. MS (ESI) m/z 582.7.
4-fluoro-N-{4-[({2-[(2-hydroxyethyl)(methyl)amino]-7-methylquinazolin-4-yl}amino)methyl]phenyl}benzamide was prepared by amination of N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (30 mg, 0.07 mmol) and 2-methylaminoethanol in dioxan following the procedure B (step 2). After purification by HPLC and solvent removal the final product (22 mg, yield, 61%) was isolated as bis-TFA salt. MS (ESI) m/z 460.5.
N-{4-[({2-[4-(1,3-benzodioxol-5-ylmethyl)piperazin-1-yl]-7-methylquinazolin-4-yl}amino)methyl]phenyl}-4-fluorobenzamide was prepared by amination of N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (30 mg, 0.07 mmol) and 1-piperonylpiperazine in dioxan following the procedure B (step 2). After purification by HPLC and solvent removal the final product (36 mg, yield, 71%) was isolated as bis-TFA salt. MS (ESI) m/z 605.7.
4-fluoro-N-[4-({[7-methyl-2-(4-pyrimidin-2-ylpiperazin-1-yl)quinazolin-4-yl]amino}methyl)phenyl]benzamide was prepared by amination of N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (30 mg, 0.07 mmol) and 1-(2-pyrimidyl)-piperazine hydrochloride and NEt3 in dioxan following the procedure B (step 2). After purification by HPLC and solvent removal the final product (18 mg, yield, 39%) was isolated as bis-TFA salt. MS (ESI) m/z 549.6.
4-fluoro-N-[4-({[2-(4-formylpiperazin-1-yl)-7-methylquinazolin-4-yl]amino}methyl)phenyl]benzamide was prepared by amination of N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (30 mg, 0.07 mmol) and 1-piperazine-carboxaldehyde in dioxan following the procedure B (step 2). After purification by HPLC and solvent removal the final product (20 mg, yield, 52%) was isolated as bis-TFA salt. MS (ESI) m/z 499.6.
ethyl 4-[4-({4-[(4-fluorobenzoyl)amino]benzyl}amino)-7-methylquinazolin-2-yl]piperazine-1-carboxylate was prepared by amination of N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (30 mg, 0.07 mmol) and 1-Ethyl-piperazine carboxylate in dioxan following the procedure B (step 2). After purification by HPLC and solvent removal the final product (23 mg, yield, 58%) was isolated as bis-TFA salt. MS (ESI) m/z 543.6.
4-fluoro-N-(4-{[(2-{4-[2-(isopropylamino)-2-oxoethyl]piperazin-1-yl}-7-methylquinazolin-4-yl)amino]methyl}phenyl)benzamide was prepared by amination of N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (30 mg, 0.07 mmol) and N-isopropyl-piperazine acetamide in dioxan following the procedure B (step 2). After purification by HPLC and solvent removal the final product (32 mg, yield, 76%) was isolated as bis-TFA salt. MS (ESI) m/z 570.7.
4-fluoro-N-{4-[({2-[(2-methoxyethyl)(methyl)amino]-7-methylquinazolin-4-yl}amino)methyl]phenyl}benzamide was prepared by amination of N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (30 mg, 0.07 mmol) and 2-methoxyethyl methylamine in dioxan following the procedure B (step 2). After purification by HPLC and solvent removal the final product (10 mg, yield, 29%) was isolated as bis-TFA salt. MS (ESI) m/z 474.6.
4-fluoro-N-{4-[({2-[(2-furylmethyl)(methyl)amino]-7-methylquinazolin-4-yl}amino)methyl]phenyl}benzamide was prepared by amination of N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (30 mg, 0.07 mmol) and methylfurfurylamine in dioxan following the procedure B (step 2). After purification by HPLC and solvent removal the final product (23 mg, yield, 62%) was isolated as bis-TFA salt. MS (ESI) m/z 496.6.
4-fluoro-N-{4-[({7-methyl-2-[methyl(2-pyridin-2-ylethyl)amino]quinazolin-4-yl}amino)methyl]phenyl}benzamide was prepared by amination of N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (30 mg, 0.07 mmol) and 2-(2-methylaminoethyl)pyridine in dioxan following the procedure B (step 2). After purification by HPLC and solvent removal the final product (33 mg, yield, 86%) was isolated as bis-TFA salt. MS (ESI) m/z 521.6.
N-[4-({[2-(4-acetyl-1,4-diazepan-1-yl)-7-methylquinazolin-4-yl]amino}methyl)phenyl]-4-fluorobenzamide was prepared by amination of N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (30 mg, 0.07 mmol) and N-acetyl homopiperazine in dioxan following the procedure B (step 2). After purification by HPLC and solvent removal the final product (29 mg, yield, 73%) was isolated as bis-TFA salt. MS (ESI) m/z 527.6.
N-{4-[({2-[2-(2-hydroxyethyl)piperidin-1-yl]-7-methylquinazolin-4-yl}amino)methyl]phenyl}benzamide was prepared by amination of N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (25 mg, 0.06 mmol) and 2-(2-hydroxyethyl)piperidine in dioxan following the procedure B (step 2). After purification by HPLC and solvent removal the final product (21 mg, yield, 65%) was isolated. MS (ESI) m/z 514.6.
N-{4-[({2-[2-(2-hydroxyethyl)piperidin-1-yl]-7-methylquinazolin-4-yl}amino)methyl]phenyl}benzamide was prepared by amination of N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (25 mg, 0.06 mmol) and 2-(R)-methyl piperazine in dioxan following the procedure B (step 2). After purification by HPLC and solvent removal the final product (19 mg, yield, 64%) was isolated. MS (ESI) m/z 485.6.
4-fluoro-N-[4-({[7-methyl-2-(4-pyrrolidin-1-ylpiperidin-1-yl)quinazolin-4-yl]amino}methyl)phenyl]benzamide was prepared by amination of N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (25 mg, 0.06 mmol) and 4-(1-pyrrolidinyl)-piperidine in dioxan following the procedure B (step 2). After purification by HPLC and solvent removal the final product (18 mg, yield, 56%) was isolated. MS (ESI) m/z 539.7.
N-[4-({[2-(4-ethylpiperazin-1-yl)-7-methylquinazolin-4-yl]amino}methyl)phenyl]-4-fluorobenzamide was prepared by amination of N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (25 mg, 0.06 mmol) and 1-ethypiperazine in dioxan following the procedure B (step 2). After purification by HPLC and solvent removal the final product (28 mg, yield, 93%) was isolated. MS (ESI) m/z 499.6.
4-fluoro-N-{4-[({7-methyl-2-[(2S)-2-(pyrrolidin-1-ylmethyl)pyrrolidin-1-yl]quinazolin-4-yl}amino)methyl]phenyl}benzamide was prepared by amination of N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (25 mg, 0.06 mmol) and S-2-(1-pyrrolidinylmethyl)-pyrrolidine in dioxan following the procedure B (step 2). After purification by HPLC and solvent removal the final product (14 mg, yield, 43%) was isolated. MS (ESI) m/z 539.7.
N-(4-{[(7-methyl-2-{[3-(4-methylpiperazin-1-yl)propyl]amino}quinazolin-4-yl)amino]methyl}phenyl)benzamide was prepared by amination of N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (25 mg, 0.06 mmol) and 1-(3-aminopropyl)-4-methylpiperazine in dioxan following the procedure B (step 2). After purification by HPLC and solvent removal the final product (32 mg, yield, 95%) was isolated. MS (ESI) m/z 542.7.
4-fluoro-N-[4-({[7-methyl-2-(methylamino)quinazolin-4-yl]amino}methyl)phenyl]benzamide was prepared by amination of N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (250 mg, 0.6 mmol) and 2 M methylamine in THF following the procedure B (step 2). After purification by HPLC and solvent removal the final product (150 mg, yield, 76%) was isolated. MS (ESI) m/z 416.2.
4-fluoro-N-[4-({[7-methyl-2-(propylamino)quinazolin-4-yl]amino}methyl)phenyl]benzamide was prepared by amination of N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (150 mg, 0.36 mmol) and propylamine in THF following the procedure B (step 2). After purification by HPLC and solvent removal the final product (150 mg, yield, 76%) was isolated. MS (ESI) m/z 444.1; mp 170-174° C.
4-fluoro-N-{4-[({7-methyl-2-[(2-pyridin-2-ylethyl)amino]quinazolin-4-yl}amino)methyl]phenyl}benzamide was prepared by amination of N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (150 mg, 0.36 mmol) and 2-ethylamino-2-pyridine in THF following the procedure B (step 2). After purification by HPLC and solvent removal the final product (63 mg, yield, 35%) was isolated. MS (ESI) m/z 507.2; mp 112-115° C.
N-{4-[({2-[(1-benzylpiperidin-4-yl)amino]-7-methylquinazolin-4-yl}amino)methyl]phenyl}-4-fluorobenzamide was prepared by amination of N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (150 mg, 0.36 mmol) and 4-amino-1-benzylpiperindine in THF following the procedure B (step 2). After purification by HPLC and solvent removal the final product (70 mg, yield, 34%) was isolated. MS (ESI) m/z 575.2; mp 135-138° C.
4-fluoro-N-{4-[({7-methyl-2-[(3S)-3-methylpiperazin-1-yl]quinazolin-4-yl}amino)methyl]phenyl}benzamide was prepared by amination of N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (150 mg, 0.36 mmol) and 4-amino-1-benzylpiperindine in THF following the procedure B (step 2). After purification by HPLC and solvent removal the final product (68 mg, yield, 39%) was isolated. MS (ESI) m/z 485; mp 200-202° C.
1N-(4-{[(2-azepan-1-yl-7-methylquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide was prepared by amination of N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (150 mg, 0.36 mmol) and aza-cycloheptane in THF following the procedure B (step 2). After purification by HPLC and solvent removal the final product (132 mg, yield, 77%) was isolated. MS (ESI) m/z 484.3; mp 135-139° C.
N-[4-({[2-(3,3-dimethylpiperazin-1-yl)-7-methylquinazolin-4-yl]amino}methyl)phenyl]-4-fluorobenzamide was prepared by amination of N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (150 mg, 0.36 mmol) and 2,2-dimethylpiperazine in THF following the procedure B (step 2). After purification by HPLC and solvent removal the final product (105 mg, yield, 59%) was isolated. MS (ESI) m/z 499.3.
4-fluoro-N-{4-[({7-methyl-2-[(2-pyrrolidin-1-ylethyl)amino]quinazolin-4-yl}amino)methyl]phenyl)}benzamide was prepared by amination of N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)-4-fluorobenzamide (150 mg, 0.36 mmol) and 2-ethylamino pyrrolidine in THF following the procedure B (step 2). After purification by HPLC and solvent removal the final product (55 mg, yield, 31%) was isolated. MS (ESI) m/z 499.1.
To a stirred suspension N-[4-(aminomethyl)phenyl]-4-bromobenzamide (590 mg, 1.41 mmol) and triethylamine (0.983 mL) in THF (6 mL) was added 7-methyl-2,4-dichloroquinazoline (300 mg, 1.41 mmol) at rt then the mixture was stirred overnight. Added CHCl3 (50 mL) and water (10 mL), collected organic layer and the solvent was removed then triturated with CH2Cl2 to give 4-bromo-N-(4-((2-chloro-7-methylquinazolin-4-ylamino)methyl)phenyl)benzamide as a white solid (670 mg, yield, 99%). MS (ESI) m/z 481.1.
To the 4-bromo-N-(4-((2-chloro-7-methylquinazolin-4-ylamino)methyl)phenyl)benzamide (150 mg, 0.311 mmol), methylamine.HCl (462 mg, 6.842 mmol) and 2-propanol (3 mL) was heated under reflux overnight. The solvent was removed and purified by combi flash chromatography using 10% MeOH in CH2Cl2/EtOAc (1:1) to give the product (65 mg, yield, 44%) mp 295-298° C.; MS (ESI) m/z 476.1; HRMS: calcd for C24H22BrN5O+H+, 476.10805; found (ESI-FTMS, [M+H]1+), 476.10943.
4-bromo-N-(4-((2-chloro-7-methylquinazolin-4-ylamino)methyl)phenyl)benzamide (150 mg, 0.311 mmol), dimethylamine (40% aqueous solution) (0.867 mL, 6.842 mmol) and THF (1 mL) was heated to 100° C. in a sealed tube overnight. The solvent was removed and purified by combi flash chromatography using 10% MeOH in CH2Cl2/EtOAc (1:1) to give the product (102 mg, yield, 67%). MS (ESI) m/z 490.2; HRMS: calcd for C25H24BrN5O+H+, 490.12370; found (ESI-FTMS, [M+H]1+), 490.12271.
To a stirred suspension N-[4-(aminomethyl)phenyl]-6-methylnicotinamide (440 mg, 0.939 mmole) and triethylamine (0.654 mL) in THF (4 mL) was added 7-methyl-2,4-dichloroquinazoline (200 mg, 0.939 mmol) at rt then the mixture was stirred overnight. Added CHCl3 (50 mL) and water (10 mL), collected organic layer and the solvent was removed then triturated with CH2Cl2 to give N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)-6-methylnicotinamide as a white solid (420 mg, yield, 100%). MS (ESI) m/z 418.2.
To the N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)-6-methylnicotinamide (180 mg, 0.431 mmol), methylamine.HCl (631 mg, 9.34 mmol) and 2-propanol (6 mL) was heated under reflux overnight. The solvent was removed and purified by combi flash chromatography using 10% MeOH in CH2Cl2/EtOAc (1:1) to give the product (75 mg, yield, 42%) mp 257-259° C.; MS (ESI) m/z 413.2; MS (ESI) m/z 207.1; MS (ESI) m/z 246.1; HRMS: calcd for C24H24N6O+H+, 413.20843; found (ESI-FTMS, [M+H]1+), 413.20848.
To the N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)-6-methylnicotinamide (180 mg, 0.431 mmol), dimethylamine (40% aqueous solution) (1.1 mL, 8.62 mmol) and THF (2 mL) was heated to 100° C. in a sealed tube overnight. The solvent was removed and purified by combi flash chromatography using 10% MeOH in CH2Cl2/EtOAc (1:1) to give the product (68 mg, yield, 37%). MS (ESI) m/z 427.3; MS (ESI) m/z 214.1; MS (ESI) m/z 234.7; HRMS: calcd for C25H26N6O+H+, 427.22408; found (ESI-FTMS, [M+H]1+), 427.22561.
To the 6-chloro-N-(4-{[(2-chloro-7-methylquinazolin-4-yl)amino]methyl}phenyl)nicotinamide 4.89 grams, 11.16 mmol), dimethylamine.HCl (10.92 grams, 133.88 mmol) and DMF (30 mL) was heated to 100° C. for 1.5 hrs. Concentrated and added water the solid was collected and triturated with methanol to give the product (4.05 grams, yield 75%) as a mono hydrochloride salt. mp 325-329° C.; MS (ESI) m/z 447.2.
To a stirred solution of 4-aminobenzylamine (285 mg, 2.33 mmol) and triethylamine (1200 mg, 11.7 mmol, 5 eq) in CHCl3 (5 mL) at rt was added 7-methyl-2,4-dichloroquinazoline (500 mg, 2.33 mmol) as solid and the mixture was allowed to stir for a minimum of 3 hours. After TLC showed only minimal amounts of starting materials remaining, THF (10 mL), HOBT (629 mg, 4.66 mmol, 2 eq), N—BOC-isonipecotic acid (600 mg, 2.62 mmol, 1.1 eq) and EDCI (670 mg, 3.5 mmol, 1.5 eq) were added in the described order and the mixture was allowed to stir overnight. For work up CHCl3 (100 mL) and water (20 mL) were added and the organic layer was separated. The aqueous layer was washed twice with CHCl3 (20 mL) and the combined organic layers were dried over MgSO4. After filtration of MgSO4 and solvent removal, the 1.5 g crude product (which was a 1:1-mixture of (4-{4-[(2-Chloro-7-methyl-quinazolin-4-ylamino)-methyl]-phenylcarbamoyl}-piperidine-1-carboxylic acid tert-butyl ester) and 4-(tert-Butoxycarbonyl-{4-[(2-chloro-7-methyl-quinazolin-4-ylamino)-methyl]-phenyl}-aminocarbonyl)-piperidine-1-carboxylic acid tert-butyl ester) was taken forward without further purification.
The crude product (1.5 g, 2.33 mmol) from procedure D (step 1) was placed in a round bottom flask with dimethylamine hydrochloride (4 g) and was dissolved in DMF (10 mL). The mixture was heated under stirring over 1-2 h to 120° C. After the reaction was completed, aqueous 1N NaOH (50 mL) and CHCl3 (100 mL) was added; the layers were separated and the aqueous layer was washed twice with CHCl3 (25 mL). Vacuum distillation of the combined organic layers led to a thick oil, which was stirred with water (30 mL) to form a solid. The solid was collected by filtration and dried at (50° C./14 mbar) to obtain 720 mg (yield, 59%) of tert-butyl 4-({[4-({[2-(dimethylamino)-7-methylquinazolin-4-yl]amino}methyl)phenyl]amino}carbonyl)piperidine-1-carboxylate. MS (ESI) e/z=521.
In a round bottom flask was added to a stirred suspension, of tert-butyl 4-({[4-({[2-(dimethylamino)-7-methylquinazolin-4-yl]amino}methyl)phenyl]amino}carbonyl)piperidine-1-carboxylate (600 mg, 1.15 mmol) in chloroform (15 mL), trifluoroacetic acid (5 mL). The mixture was stirred at room temperature for two hours until deprotection was completed. Solvents were removed in vacuo and the residue was taken up 5 mL ACN. When the solution was basified with 5N NaOH, precipitation of a solid was observed. The precipitate was collected by filtration washed with water (1 mL). After drying over night at 50° C./14 mbar, 280 mg (yield, 66%) of the product as off-white solid was obtained. MS (ESI) m/z 421.
In a round bottom flask were dissolved N-[4-({[2-(dimethylamino)-7-methylquinazolin-4-yl]amino}methyl)phenyl]piperidine-4-carboxamide (280 mg, 0.66 mmol) and the appropriate aldehyde or ketone (1.33 mmol, 2 eq) in methanol (3 mL). A suspension of NaBH3CN (84 mg, 1.33 mmol, 2 eq) and ZnCl2 (90 mg, 0.66 mmol, 1 eq) in methanol (1 mL) was added slowly. The reaction was stirred for 2-6 h; the solvents were removed under reduced pressure and 1N NaOH, (2 mL); and THF/ethyl acetate (1:1) (10 mL) were added. The organic layer was separated and the aqueous layer was washed twice with 5 mL (1:1) THF/ethyl acetate. The combined organic layers were dried over MgSO4. After removal of the drying agent by filtration, the solvents were distilled on a rota-evaporator and the crude compound was purified by preparative HPLC (high pressure liquid chromatography) using ACN/water/NH3-gradients as eluent or column chromatography with CH2Cl2/MeOH/NH3 to give the N-alkyl piperidine analogs in yields between 65-83%.
Some of the products were converted to the bis-HCl salts by dissolving in MeOH and addition of 4M HCl in dioxan (0.5 mL). The bis-HCl salt was obtained after solvent removal in vacuo.
N-[4-({[2-(dimethylamino)-7-methylquinazolin-4-yl]amino}methyl)phenyl]piperidine-4-carboxamide (25 mg, 0.06 mmol) was N-alkylated according to procedure D (step 4) using 37% aq formaldehyde solution to give product (15 mg, yield, 56%). MS (ESI) m/z 433.3.
N-[4-({[2-(dimethylamino)-7-methylquinazolin-4-yl]amino}methyl)phenyl]piperidine-4-carboxamide (25 mg, 0.06 mmol) was N-alkylated according to procedure D (step 4) using isobutyraldehyde to give product (18 mg, yield, 61%) MS (ESI) m/z 475.3.
N-[4-({[2-(dimethylamino)-7-methylquinazolin-4-yl]amino}methyl)phenyl]piperidine-4-carboxamide (25 mg, 0.06 mmol) was N-alkylated according to procedure D (step 4) using cyclohexanon to give product (7 mg, yield, 23%) MS (ESI) m/z 501.3.
N-[4-({[2-(dimethylamino)-7-methylquinazolin-4-yl]amino}methyl)phenyl]piperidine-4-carboxamide (25 mg, 0.06 mmol) was N-alkylated according to procedure D (step 4) using furfural to give product (20 mg, yield, 65%). MS (ESI) m/z 499.
N-[4-({[2-(dimethylamino)-7-methylquinazolin-4-yl]amino}methyl)phenyl]piperidine-4-carboxamide (230 mg, 0.55 mmol) was N-benzylated according to procedure D (step 4) using p-toluoylaldehyde to give product (114 mg, yield, 35%) as bis hydrochloride salt. MS (ESI) m/z 523.
N-[4-({[2-(dimethylamino)-7-methylquinazolin-4-yl]amino}methyl)phenyl]piperidine-4-carboxamide (25 mg, 0.06 mmol) was N-alkylated according to procedure D (step 4) using 2-imidazol-carbaldehyde to give product (18 mg, yield, 60%). MS (ESI) m/z 499.
N-[4-({[2-(dimethylamino)-7-methylquinazolin-4-yl]amino}methyl)phenyl]piperidine-4-carboxamide (25 mg, 0.06 mmol) was N-alkylated according to procedure D (step 4) using butanal to give product (18 mg, yield, 62%). MS (ESI) m/z 475.
N-[4-({[2-(dimethylamino)-7-methylquinazolin-4-yl]amino}methyl)phenyl]piperidine-4-carboxamide (200 mg, 0.48 mmol) was N-benzylated according to procedure D (step 4) using p-anisaldehyde to give product (68 mg, yield, 23%) as bis hydrochloride salt. MS (ESI) m/z 539.4.
N-[4-({[2-(dimethylamino)-7-methylquinazolin-4-yl]amino}methyl)phenyl]piperidine-4-carboxamide (280 mg, 0.66 mmol) was N-benzylated according to procedure D (step 4) using 4-fluorobenzaldehyde to give product (219 mg, yield, 55%) as bis hydrochloride salt. MS (ESI) m/z 527.4.
N-[4-({[2-(dimethylamino)-7-methylquinazolin-4-yl]amino}methyl)phenyl]piperidine-4-carboxamide (230 mg, 0.55 mmol) was N-benzylated according to procedure D (step 4) using 2-fluorobenzaldehyde to give product (122 mg, yield, 37%) as bis hydrochloride salt. MS (ESI) m/z 527.4.
N-[4-({[2-(dimethylamino)-7-methylquinazolin-4-yl]amino}methyl)phenyl]piperidine-4-carboxamide (230 mg, 0.55 mmol) was N-benzylated according to procedure D (step 4) using 4-chlorobenzaldehyde to give product (110 mg, yield, 32%) as bis hydrochloride salt. MS (ESI) m/z 543.4.
N-[4-({[2-(dimethylamino)-7-methylquinazolin-4-yl]amino}methyl)phenyl]piperidine-4-carboxamide (200 mg, 0.48 mmol) was N-benzylated according to procedure D (step 4) using 2,4-difluorobenzaldehyde to give product (60 mg, yield, 23%). MS (ESI) m/z 545.5.
N-[4-({[2-(dimethylamino)-7-methylquinazolin-4-yl]amino}methyl)phenyl]piperidine-4-carboxamide (200 mg, 0.48 mmol) was N-benzylated according to procedure D (step 4) using 3,4-difluorobenzaldehyde to give product (108 mg, yield, 41%). m.p.: 97-99° C. MS (ESI) m/z 545.5.
N-[4-({[2-(dimethylamino)-7-methylquinazolin-4-yl]amino}methyl)phenyl]piperidine-4-carboxamide (200 mg, 0.48 mmol) was N-benzylated according to procedure D (step 4) using 4-trifluoromethylbenzaldehyde to give product (88 mg, yield, 32%). m.p. 126-128° C. MS (ESI) m/z 577.5.
N-[4-({[2-(dimethylamino)-7-methylquinazolin-4-yl]amino}methyl)phenyl]piperidine-4-carboxamide (200 mg, 0.48 mmol) was N-alkylated according to procedure D (step 4) using 4-pyridinecarboxaldehyde and subsequently converted to the bis-HCl salt following procedure M to give the product (102 mg, yield, 42%). HRMS: calcd for C30H35N7O+H+, 510.29758; found (ESI-FTMS, [M+H]1+), 510.29608.
N-[4-({[2-(dimethylamino)-7-methylquinazolin-4-yl]amino}methyl)phenyl]piperidine-4-carboxamide (200 mg, 0.48 mmol) was N-benzylated according to procedure D (step 4) using 2-chloro-4-fluorobenzaldehyde and subsequently converted to the bis-HCl salt to give the product (65 mg, yield, 24%) HRMS: calcd for C31H34ClFN6O+H+, 561.25394; found (ESI-FTMS, [M+H]1+), 561.25461.
N-[4-({[2-(dimethylamino)-7-methylquinazolin-4-yl]amino}methyl)phenyl]piperidine-4-carboxamide (200 mg, 0.48 mmol) was N-alkylated according to procedure D (step 4) using 6-chloro-3-pyridinecarbaldehyde and subsequently converted to the bis-HCl salt to give the product (54 mg, yield, 18%) MS (ESI) m/z 544.4.
N-[4-({[2-(dimethylamino)-7-methylquinazolin-4-yl]amino}methyl)phenyl]piperidine-4-carboxamide (200 mg, 0.48 mmol) was N-benzylated according to procedure D (step 4) using 2,4,6-trifluorobenzaldehyde and subsequently converted to the bis-HCl salt to give the product (102 mg, yield, 34%). MS (ESI) m/z 563.4.
N-[4-({[2-(dimethylamino)-7-methylquinazolin-4-yl]amino}methyl)phenyl]piperidine-4-carboxamide (200 mg, 0.48 mmol) was N-benzylated according to procedure D (step 4) using 3-fluorobenzaldehyde and subsequently converted to the bis-HCl salt to give the product (128 mg, yield, 45%). MS (ESI) m/z 527.4.
N-[4-({[2-(dimethylamino)-7-methylquinazolin-4-yl]amino}methyl)phenyl]piperidine-4-carboxamide (200 mg, 0.48 mmol) was N-benzylated according to procedure D (step 4) using 2,5 difluorobenzaldehyde and subsequently converted to the bis-HCl salt to give the product (130 mg, yield, 44%). MS (ESI) m/z 545.3.
N-[4-({[2-(dimethylamino)-7-methylquinazolin-4-yl]amino}methyl)phenyl]piperidine-4-carboxamide (200 mg, 0.48 mmol) was N-benzylated according to procedure D (step 4) using 4-chloro-3-fluorobenzaldehyde and subsequently converted to the bis-HCl salt to give the product (176 mg, yield, 58%). MS (ESI) m/z 561.4.
To a stirred solution of 4-nitrobenzylamine hydrochloride (444 mg, 2.36 mol) and triethylamine (1 mL) in CHCl3 (5 mL) at rt was added 7-methyl-2,4-dichloroquinazoline (500 mg, 2.36 mol). The mixture was stirred for one hour. After TLC showed the complete disappearance of the 2,4-dichloroquinazoline CHCl3 (20 mL) and water (15 mL). The layers were separated and the chloroform layer was dried over MgSO4. After removal of MgSO4 by filtration and evaporation of solvents the crude (2-Chloro-7-methyl-quinazolin-4-yl)-(4-nitro-benzyl)-amine (660 mg, yield 85%) was taken forward without further purification.
The (2-Chloro-7-methyl-quinazolin-4-yl)-(4-nitro-benzyl)-amine (600 mg, 1.83 mmol) was taken up in a round bottom flask and was dissolved in DMF (6 mL) and methylamine hydrochloride (2 g, 29.6 mmol, 16 eq) was added. The mixture was heated over one hour to 100° C. After reaction was completed the solvents were removed in vacuo and the mixture was basified with 5 N NaOH and extracted with TEA/THF mixtures. The combined org layers were dried over MgSO4. Filtration and distillation under reduced pressure led to the crude material, which was purified by column chromatography (CH2Cl2/MeoH/NH3) to give the product (540 mg, yield, 91%). MS (ESI) m/z 324.
The (2-N-dimethylamino-7-methyl-quinazolin-4-yl)-(4-nitro-benzyl)-amine (540 mg, 1.67 mmol) was dissolved in THF (30 mL) and Pd—C (10% wet) (100 mg) was added. and the mixture hydrogenated under 1 atm pressure for 24 h. When completed MeOH (500 mL) were added and the mixture was filtered over diatomaceous earth. The solvent were removed in vacuum to obtain (4-Amino-benzyl)-(2-methylamino-7-methyl-quinazolin-4-yl)-amine, which was suspended in THF (10 mL) and NEt3 (0.4 mL) and cooled to 0 C. 6-cloronicontinoyl chloride (280 mg, 1.59 mmol) was added. After completion 1N NaOH (0.5 mL) was added and the layers were separated. The org layer was extracted twice with THF/ethyl acetate (10 mL) and the combined organic layers were dried over MgSO4. The crude material was purified by column chromatography using CH2Cl2/MeOH/NH3 to give product (230 mg, yield, 31%). MS (ESI) m/z 433.2.
To a stirred solution of 4-nitrobenzylamine hydrochloride (832 mg, 4.41 mmol) and triethylamine (1 mL) in CHCl3 (5 mL) at rt was added 8-methyl-2,4-dichloroquinazoline (850 mg, 4.01 mmol). TLC showed the complete disappearance of the 2,4-dichloroquinazoline after two hours, and a white solid precipitated. Water (15 mL) was added and the solid was collected by filtration and washed with water (0.5 mL). After drying over night at 50 C/14 mbar the 2-Chloro-8-methyl-quinazolin-4-yl)-(4-nitro-benzyl)-amine (900 mg, yield 68%) was obtained as white solid. MS (ESI) m/z 329 (is taken forward without further purification).
The (2-Chloro-8-methyl-quinazolin-4-yl)-(4-nitro-benzyl)-amine (700 mg, 2.12 mmol) was taken up in a sealed tube and was suspended in 2M H2NMe in THF-solution (6 mL) and was heated overnight to 100° C. After reaction was completed the solvents were removed in vacuo and the crude material was purified by column chromatography (CH2Cl2 MeOH NH3) to give the (2-N-dimethylamino-8-methyl-quinazolin-4-yl)-(4-nitro-benzyl)-amine (150 mg, yield, 21%). MS (ESI) m/z 324.
The (2-methylamino-7-methyl-quinazolin-4-yl)-(4-nitro-benzyl)-amine (540 mg, 1.67 mmol) was dissolved in THF (30 mL) and Pd—C (10% wet) (100 mg) was added and the mixture hydrogenated under 1 atm pressure for 24 h. When completed MeOH (500 mL) were added and the mixture was filtered over diatomaceous earth. The solvent were removed in vacuum to obtain (4-Amino-benzyl)-(2-methylamino-8-methyl-quinazolin-4-yl)-amine (72 mg, yield, 50%).
In a vial equipped with stirring bar was dissolved (4-Amino-benzyl)-(2-methylamino-8-methyl-quinazolin-4-yl)-amine (13 mg, 0.06 mmol) and NEt3 (0.03 mL) in THF (1.3 mL) The mixture was stirred at rt and 4-bromobenzoyl chloride (50 mg, 0.28 mmol) was added. After completion of the reaction, the solvents were removed by nitrogen purge and the residue was dissolved in DMSO (2 mL), filtered and injected to a preparative HPLC (high pressure liquid chromatography) using ACN/water/TFA3-gradients. The product (9 mg, yield, 25%) was obtained after solvent removal. MS (ESI) m/z 476.
In a vial equipped with stirring bar was dissolved (4-Amino-benzyl)-(2-methylamino-8-methyl-quinazolin-4-yl)-amine (13 mg, 0.06 mmol) and NEt3 (0.03 mL) in THF (1.3 mL). The mixture was stirred at rt and 4-cyanobenzoyl chloride (50 mg, 0.28 mmol) was added. After completion of the reaction, the solvents were removed by nitrogen purge and the residue was dissolved in DMSO (2 mL), filtered and injected to a preparative HPLC (high pressure liquid chromatography) using ACN/water/TFA3-gradients. The product (8 mg, yield, 25%) was obtained after solvent removal. MS (ESI) m/z 423.
To the mixture of (S)-∝-methyl-4-nitrobenzylamine (1.14 grams, 4.69 mmol) and tetrahydrofuran (15 mL) was added triethylamine (2.61 mL) and 7-methyl-2,4-dichloroquinazoline at 0° C. then the mixture was stirred at room temperature overnight. The solvent was removed under reduced pressure and the residue was diluted with methylene chloride then washed with water and brine. Dried over sodium sulfate, filtered, concentrated and dried to give the product (1.7 grams, yield, 100%). MS (ESI) m/z 343.1.
To the 2-chloro-7-methyl-N-[(1S)-1-(4-nitrophenyl)ethyl]quinazolin-4-amine (1.6 grams, 4.65 mmol) and 2-propanol/THF (1:1) 16 mL was added dimethylamine. HCl and heated under reflux overnight. Added methylene chloride and water, collected organic and the solvent was removed under vacuo then the residue was purified by combi flash chromatography using 10% MeOH in CH2Cl2 to give the product (1.2 g, yield, 73%). MS (ESI) m/z 352.2.
To the mixture of N2,N2,7-trimethyl-N4-[(1S)-1-(4-nitrophenyl)ethyl]quinazoline-2,4-diamine (1.1 g, 3.13 mmole) and THF/MeOH (1:1) (14 mL) was added of 10% Pd/C (1.1 g.) is stirred overnight under balloon pressure (H2). Filtration thru diatomaceous earth and removal of solvent afforded the product. (995 mg, yield, 99%). MS (ESI) m/z 322.2.
To the boc-isonipecotic acid (493 mg, 2.15 mmol) acid in DMF (3 mL) was added HOBT (580 mg, 4.29 mmol) and EDAC (618 mg, 3.23 mmol) then the mixture was stirred at room temperature for 1 hr. and added N4-[(1S)-1-(4-aminophenyl)ethyl]-N2,N2,7-trimethylquinazoline-2,4-diamine (460 mg, 1.43 mol) and stirred overnight. The solvent was removed and purified by combi flask chromatography using 10% MeOH in CH2Cl2 to give the product (430 mg, yield, 56%). MS (ESI) m/z 533.4.
To the tert-butyl 4-({[4-((1S)-1-{[2-(dimethylamino)-7-methylquinazolin-4-yl]amino}ethyl)phenyl]amino}carbonyl)piperidine-1-carboxylate (390 mg, 0.733 mmol) and Chloroform (3 mL) was added trifluoroacetic acid (565 uL) at 0° C. and stirred at room temp. for 2 hrs. The solvent was removed and the residue was basified with 1N NaOH and the product was collected by filtration (280 mg; yield, 88%). MS (ESI) m/z 433.4.
To the [4-((1S)-1-{[2-(dimethylamino)-7-methylquinazolin-4-yl]amino}ethyl)phenyl]piperidine-4-carboxamide (10 mg, 0.231 mmol) in MeOH (1.5 mL) was added 3,4-difluorobenzaldehyde (66 mg, 0.462 mmol) was added NaBH3CN (29 mg, 0.462 mmol) and ZnCl2 (32 mg, 0.231 mmol). The mixture was stirred for 4 hrs and the solvent was removed then purified by combi flask chromatography using 10% MeOH in CH2Cl2 then dissolved in MeOH (1 mL) and added 4N HCl in Dioxane (1 mL). Stirred for 1 hr. and the solvent was removed and the residue was triturated with ether to give the product as a bis hydrochloride salt. (78 mg, yield, 60%). MS (ESI) m/z 280.2; HRMS: calcd for C32H36F2N6O+H+, 559.29914; found (ESI-FTMS, [M+H]1+), 559.30045.
The (S) alpha methyl-4-nitrobenzylamine hydrochloride (7.4 g, 36.6 mmol) was dissolved in THF (100 mL) and a mixture of triethylamine (excess) and di tert butyl dicarbonate (12.0 g, 55.0 mmol) was added. The reaction mixture was heated at 50° C. overnight. At the end, reaction mixture was quenched with water (25 mL) and extracted with ethyl acetate. The organics were dried over magnesium sulfate, filtered and concentrated in vacuo. The oil crystallized upon standing and was collected with hexanes and the white crystals were dried in a vacuum oven to yield (S)-tert-butyl 1-(4-nitrophenyl)ethylcarbamate (9.2 g; 95%; (M−H)-266.2).
To a solution of (S)-tert-butyl 1-(4-nitrophenyl)ethylcarbamate (9.2 g, 34.6 mmol) in ethanol (184 mL) and water (92 mL) was treated with iron powder (5.5 g, 100 mol) and ammonium chloride (16.1 g, 303 mmol) and the mixture was refluxed overnight. The reaction was filtered through diatomaceous earth and the filtrate was treated with water and extracted with ethyl acetate. The organics were dried with potassium carbonate filtered and concentrated in vacuo. The resulting oil was dissolved in dichloromethane and passed through a pad of hydrous magnesium silicate and concentrated in vacuo to yield (S)-tert-butyl 1-(4-aminophenyl)ethylcarbamate as a yellow oil (8.1 g; 98%; (M+H)−237.2).
To a solution of (S)-tert-butyl 1-(4-aminophenyl)ethylcarbamate (440 mg, 1.86 mmol) in dichloromethane (7.0 mL) and triethylamine (excess) was added 6-chloronicotinoyl chloride (380 mg, 2.2 mmol) and the reaction stirred at room temperature overnight. White precipitate formed and was collected by filtration, the solids washed with dichloromethane and dried in a vacuum oven to yield (S)-tert-butyl 1-(4-(6-chloronicotinamido)phenyl)ethylcarbamate (600 mg; 86%; (M+H)−376.1).
(S)-tert-butyl 1-(4-(6-chloronicotinamido)phenyl)ethylcarbamate (600 mg, 1.6 mmol) was dissolved in dichloromethane (5.0 mL) and excess TFA (ca. 5.0 mL) was added and stirred at room temperature for 4 h. Concentrated in vacuo to an oil then dissolved in water and filtered off insolubles. The filtrate was basified with 5N sodium hydroxide to precipitate a white solid. Collected by filtration and wash with water and dried in vacuum oven to yield (S)—N-(4-(1-aminoethyl)phenyl)-6-chloronicotinamide (270 mg; 61%; (M−H)−274.1).
To a solution of (S)—N-(4-(1-aminoethyl)phenyl)-6-chloronicotinamide (250 mg, 0.9 mmol) in DMF (3.0 mL) and triethylamine was added 2,4-dichloro-7-methylquinazoline (215 mg, 1.0 mmol) and stirred at room temperature overnight. The reaction was poured into ice/water to precipitate a solid. Separated solid was collected by filtration and wash with water. Purified by Dichloromethane/hydrous magnesium silicate filtration and concentrated in vacuo to yield (S)-6-chloro-N-(4-(1-(2-chloro-7-methylquinazolin-4-ylamino)ethyl)phenyl)nicotinamide as a yellow foam (400 mg; 98%; (M+H)−452.2).
(S)-6-chloro-N-(4-(1-(2-chloro-7-methylquinazolin-4-ylamino)ethyl)phenyl)nicotinamide (400 mg, 0.88 mmol) was dissolved DMF (3.0 mL) and added excess dimethylamine hydrochloride (ca. 400 mg) and microwave at 110° C. for 10 min. Cooled and poured onto ice/water to precipitate a white solid. Collect by filtration washed and dried crude in vacuum oven. Purified by silica gel column to yield (S)-6-chloro-N-(4-(1-(2-(dimethylamino)-7-methylquinazolin-4-ylamino)ethyl)phenyl)nicotinamide dihydrochloride salt (240 mg; 54%; (M+H) 461.2).
This compound was prepared by following the procedure as outlined in Example 270, steps 1-5 starting from 7-methyl-2,4-dichloroquinazoline.
Following the procedure as outlined in Example 270, step 6 and starting with (R)-6-chloro-N-(4-(1-(2-chloro-7-methylquinazolin-4-ylamino)ethyl)phenyl)nicotinamide (380 mg, 0.84 mmol) afforded (R)-6-chloro-N-(4-(1-(2-(dimethylamino)-7-methylquinazolin-4-ylamino)ethyl)phenyl)nicotinamide dihydrochloride salt (270 mg; 49%; (M+H) 461.2).
This compound was prepared by following the procedure as outlined in Example 270, step 3. Starting with (S)-tert-butyl 1-(4-aminophenyl)ethylcarbamate (500 mg, 2.12 mmol) and 4-fluorobenzoyl chloride (0.3 mL, 2.5 mmol) the reaction afforded (S)-tert-butyl 1-(4-(4-fluorobenzamido)phenyl)ethylcarbamate (550 mg; 73%; (M−H) 357.3).
This compound was prepared by following the procedure as outlined in Example 270, step 4. Starting with (S)-tert-butyl 1-(4-(4-fluorobenzamido)phenyl)ethylcarbamate (500 mg, 1.4 mmol) afforded (S)—N-(4-(1-aminoethyl)phenyl)-4-fluorobenzamide (320 mg; 89%; (M−H) 257.2).
This compound was prepared by following the procedure as outlined in Example 270, step 5. Starting with (S)—N-(4-(1-aminoethyl)phenyl)-4-fluorobenzamide (250 mg, 0.96 mmol) afforded (S)—N-(4-(1-(2-chloro-7-methylquinazolin-4-ylamino)ethyl)phenyl)-4-fluorobenzamide (420 mg; 99%; (M+H) 435.2).
This compound was prepared by following the procedure as outlined in Example 270, step 5. Starting from (S)—N-(4-(1-(2-chloro-7-methylquinazolin-4-ylamino)ethyl)phenyl)-4-fluorobenzamide (400 mg, 0.92 mmol) afforded (S)—N-(4-(1-(2-(dimethylamino)-7-methylquinazolin-4-ylamino)ethyl)phenyl)-4-fluorobenzamide hydrochloride salt (250 mg; 56%; (M+H) 444.3).
This compound was prepared by following the procedure as outlined in Example 272, steps 1-3.
Following the same procedure as outlined in Example 272 step 4 and starting from (R)—N-(4-(1-(2-chloro-7-methylquinazolin-4-ylamino)ethyl)phenyl)-4-fluorobenzamide (380 mg, 0.87 mmol) afforded (R)—N-(4-(1-(2-(dimethylamino)-7-methylquinazolin-4-ylamino)ethyl)phenyl)-4-fluorobenzamide hydrochloride salt (270 mg; 61%; (M+H) 444.3).
This compound was prepared by following the procedure as outlined in Example 270, steps 1-4.
Following the same procedure as outlined in Example 270 step 5 and starting from (S)—N-(4-(1-aminoethyl)phenyl)-6-chloronicotinamide (100 mg, 0.36 mmol) and 2,4-dichloro-8-methylquinazoline (77 mg, 0.36 mmol) afforded (S)-6-chloro-N-(4-(1-(2-chloro-8-methylquinazolin-4-ylamino)ethyl)phenyl)nicotinamide (150 mg; 71%; (M+H) 452.2).
Following the same procedure as outlined in Example 270 step 6. (S)-6-chloro-N-(4-(1-(2-chloro-8-methylquinazolin-4-ylamino)ethyl)phenyl)nicotinamide (100 mg 0.22 mmol) afforded (S)-6-chloro-N-(4-(1-(2-(dimethylamino)-8-methylquinazolin-4-ylamino)ethyl)phenyl)nicotinamide dihydrochloride salt (75 mg; 64%; (M+H) 461.3).
This compound was prepared by following the procedure as outlined in Example 270, steps 1-4.
Following the same procedure as outlined in Example 270 step 5 and starting from (S)—N-(4-(1-aminoethyl)phenyl)-6-chloronicotinamide (100 mg, 0.36 mmol) and 2,4-dichloro-6-methylquinazoline (77 mg, 0.36 mmol) afforded (S)-6-chloro-N-(4-(1-(2-chloro-6-methylquinazolin-4-ylamino)ethyl)phenyl)nicotinamide. (110 mg; 70%; (M+H) 452.2).
Following the same procedure as outlined in Example 270 step 6 and starting from (S)-6-chloro-N-(4-(1-(2-chloro-8-methylquinazolin-4-ylamino)ethyl)phenyl)nicotinamide (100 mg 0.22 mmol) afforded (S)-6-chloro-N-(4-(1-(2-(dimethylamino)-6-methylquinazolin-4-ylamino)ethyl)phenyl)nicotinamide.dihydrochloride salt (90 mg; 77%; (M+H) 461.3).
This compound was prepared by following the procedure as outlined in Example 272, steps 1-2.
This compound was prepared by following the procedure as outlined in Example 270, step 5. Starting with (S)—N-(4-(1-aminoethyl)phenyl)-4-fluorobenzamide (280 mg, 1.07 mmol) and 2,4-dichloro-8-methylquinazoline (230 mg, 1.07 mmol afforded (S)—N-(4-(1-(2-chloro-8-methylquinazolin-4-ylamino)ethyl)phenyl)-4-fluorobenzamide (470 mg; 99%; (M+H) 435.1).
This compound was prepared by following the procedure as outlined in Example 270, step 5. Starting from (S)—N-(4-(1-(2-chloro-8-methylquinazolin-4-ylamino)ethyl)phenyl)-4-fluorobenzamide (200 mg, 0.46 mmol) and methylamine hydrochloride (excess) afforded (S)—N-(4-(1-(2,8-dimethylquinazolin-4-ylamino)ethyl)phenyl)-4-fluorobenzamide dihydrochloride salt (115 mg; 50%; (M+H) 430.2).
This compound was prepared from 8-methyl-2,4-dichloroquinazoline by following the procedure as outlined in Example 270, steps 1 to 5.
Starting from (R)—N-(4-(1-(2-chloro-8-methylquinazolin-4-ylamino)ethyl)phenyl)-4-fluorobenzamide (310 mg, 0.71 mmol) and methylamine hydrochloride (excess) afforded (S)—N-(4-(1-(2,8-dimethylquinazolin-4-ylamino)ethyl)phenyl)-4-fluorobenzamide hydrochloride salt (101 mg; 27%; (M+H) 430.2).
Compounds of the present invention can be tested according to the protocol described.
Background cell line: SW480 colorectal carcinoma DNA constructs:
1) Tcf-4-Luc: Tcf-4 binding sites driving Firefly Luciferase in the pGL3 vector—to measure the activity of β-catenin/Tcf-4;
2) SV40-Luc: SV40 binding site driving Firefly Luciferase in the pGL3 vector—as a control for non-specific inhibitors; and
3) SV40-R-Luc: SV40 binding site driving Renilla Luciferase in the pGL3 vector—as an internal control for cell number and toxicity.
This screen uses 3 cell lines derived from SW480 and selected to contain the above luciferase reporters integrated into their chromosomes as follows:
Tcf 33.13—Tcf-4-Luc and SV40-R-Luc
Tcf 22C11—Tcf-4-Luc and SV40-R-Luc
SV 5A8—SV40-Luc and SV40-R-Luc
Cells: Tcf22C11 and SV5A8 were grown in the presence of 500 μg/ml G418 and Tcf 33.13 was grown in the presence of 500 μg/ml G418+125 μg/ml Zeocin to maintain the integrated reporters. The cells were trypsinized and plated at 1×104 cells/well in opaque 96 well plates.
Compounds: 20-24 hours after cell plating, compounds were added at titrated concentrations between 10 μg/ml and 10 μg/ml. The compounds were diluted as follows: The compounds were stored frozen in dimethyl sulfoxide (DMSO) at 10 μg/ml. Compounds were initially titrated in DMSO to make a 1000×DMSO stock solution. Compounds were then diluted 1:25 into media/DMSO (DMEM+10% FBS+9% DMSO). Using the Biomek Multimek, the diluted compounds were mixed well and immediately added to the plated cells in a 1:40 dilution. The final concentration of DMSO in the assay plates was 0.316% DMSO.
Assay Readout: The luciferase signal was detected using a luciferase detection kit from Promega. Until December 2003, Promega's Dual-Luciferase® Reporter (DLR™) Assay System was used to detect luciferase activity. Briefly, after 24 hours of incubation with compound, culture media was removed by aspiration and 20 ul of passive lysis buffer (Promega) per well was added. The plates were shaken for 15 min. Firefly substrate was then added and the resulting luminescence was immediately read on a CCD camera imaging device or a luminometer. After quantifying the firefly luminescence, the reaction was quenched, and the Renilla luciferase reaction was initiated simultaneously by adding Stop & Glo® Reagent to the same sample. Renilla luciferase was then quantified using the CCD camera or luminometer.
Promega's Dual-Glo™ Luciferase Assay System can be used for luciferase detection. It was similar to the DLR Assay, however, unlike the DLR which has to be read immediately due to decaying signal, the signal in the Dual-Glo™ Luciferase Assay was stable for up to 2 hours. Briefly, after 24 hours of incubation with compound, culture media was removed by aspiration and 75 ul of fresh medium was added. Add 75 ul of Dual-Glo Luciferase Reagent (Promega) to each well and mix by shaking for 10 minutes on a plate shaker. Then, measure the firefly luminescence on a CCD camera imaging device or a luminometer. After quantifying the firefly luminescence, add 75 ul of Dual-Glo Stop & Glo Reagent (Promega) to each well and mix for 10 minutes. Renilla luciferase was then quantified using the CCD camera or luminometer.
This assay was meant to confirm primary leads and determine IC50s. Compounds were tested at titrated dilutions. The raw data for this assay was reported in protocol 2463-B. Protocol 2463-C contains the IC50 calculations based on the raw data. The data calculations were done as follows:
The data was expressed as % of control for both Firefly and Renilla Luciferase. The ratio of FF/Renilla (F/R) was then calculated and averaged between duplicate plates. The data was then plotted using LSW Data Analysis in Microsoft Excel. IC50s were determined by plotting the data in the LSW Data Analysis program using model 33. The IC50s (nM) for all three cell lines were reported.
RKE/b-catenin in vivo growth assay: Athymic nude balb/c nu/nu mice (Charles River Laboratories), housed according to the Institute of Laboratory Animal Resources standards, age 8-10 weeks and weighing 20-25 grams were injected subcutaneously on the right side with 5 million RKE/b-catenin cells derived from tissue culture in a 0.2 cc.volume. 4 days following inoculation, the mice were randomized into groups of 10-12 mice, with the mean tumor volume approximately 200 mg. Treatment begins on day 0 of staging and the tumor size was monitored twice weekly with the use of calipers and the formula [L×W×W/2]. Mice were evaluated daily for signs of toxicity.
The compounds of the present invention were tested according to the protocol described. Results were required as IC50 values as shown in Table 1.
All references cited herein are incorporated herein by reference in their entirety and for all purposes to the same extent as if each individual publication or patent or patent application is specifically and individually indicated to be incorporated by reference in its entirety for all purposes. To the extent publications and patents or patent applications incorporated by reference contradict the disclosure contained in the specification, the specification is intended to supercede and/or take precedence over any such contradictory material.
All numbers expressing quantities of ingredients, reaction conditions, analytical results and so forth used in the specification and claims are to be understood as being modified in all instances by the term “about.” Accordingly, unless indicated to the contrary, the numerical parameters set forth in the specification and attached claims are approximations that may vary depending upon the desired properties sought to be obtained by the present invention. At the very least, and not as an attempt to limit the application of the doctrine of equivalents to the scope of the claims, each numerical parameter should be construed in light of the number of significant digits and ordinary rounding approaches.
Modifications and variations of this invention can be made without departing from its spirit and scope, as will be apparent to those skilled in the art. The specific embodiments described herein are offered by way of example only and are not meant to be limiting in any way. It is intended that the specification and examples be considered as exemplary only, with a true scope and spirit of the invention being indicated by the following claims.
This application claims the benefit of priority under 35 U.S.C. §119(e) to U.S. Patent Application Ser. No. 60/879,837 filed on Jan. 11, 2007, which is hereby incorporated by reference in its entirety.
| Number | Date | Country | |
|---|---|---|---|
| 60879837 | Jan 2007 | US |
