(Aminoiminomethyl) amino) alkane-carboxamides and their applications in therapy

Description

This application is a 371 of PCT/EP99/10468, filed Dec. 30, 1999.

The present invention relates to the use of derivatives of the ((aminoiminomethyl)amino)alkane-carboxamide type in the treatment of pathologies associated with insulin resistance syndrome.

The present invention therefore provides pharmaceutical compositions comprising as active principle a compound of general formula (I)

in which:

R

1

is selected from one of the following groups:

H;

(C

1

-C

20

)alkyl substituted or unsubstituted by one or more of the following groups:

amino, hydroxyl, (C

1

-C

5

)alkyl, (C

1

-C

5

)alkoxy, (C

1

-C

5

)alkylthio, (C

1

-C

5

)alkylamino, trifluoromethyl;

R

2

and R

3

are selected independently from

H;

(C

3

-C

8

)cycloalkyl substituted or unsubstituted by one or more of the following groups:

amino, hydroxyl, (C

1

-C

5

) alkyl, (C

1

-C

5

) alkoxy, (C

1

-C

5

)alkylthio, (C

1

-C

5

)alkylamino, trifluoromethyl;

(C

3

-C

8

)heterocycloalkyl containing one or more heteroatoms selected from N, O and S and substituted or unsubstituted by one or more of the following groups:

amino, hydroxyl, (C

1

-C

5

)alkyl, (C

1

-C

5

)alkoxy, (C

1

-C

5

)alkylthio, (C

1

-C

5

)alkylamino, trifluoromethyl; a nitrogen atom can additionally be substituted by a (C

6

-C

14

) aryl, (C

6

-C

14

) acyl or (C

1

-C

5

) alkyl group;

(C

6

-C

14

)aryl substituted or unsubstituted by one or more of the following groups:

amino, hydroxyl, halogen, (C

1

-C

5

)alkyl, (C

1

-C

5

) alkoxy, (C

1

-C

5

) alkylthio, (C

1

-C

5

) alkylamino, trifluoromethyl, cyano;

(C

1

-C

13

)heteroaryl containing one or more heteroatoms selected from N, O and S and substituted or unsubstituted by one or more of the following groups:

amino, hydroxyl, halogen, (C

1

-C

5

)alkyl, (C

1

-C

5

) alkoxy, (C

1

-C

5

)alkylthio, (C

1

-C

5

) alkylamino, trifluoromethyl, cyano; a nitrogen atom can additionally be substituted by a (C

6

-C

14

)aryl, (C

6

-C

14

)acyl or (C

1

-C

5

) alkyl group;

(C

6

-C

14

)aryl-(C

1

-C

5

)alkyl substituted or unsubstituted by one or more of the following groups:

amino, hydroxyl, halogen, (C

1

-C

5

)alkyl, (C

1

-C

5

)alkoxy, (C

1

-C

5

)alkylthio, (C

1

-C

5

)alkylamino, trifluoromethyl, cyano;

and A is selected from the groups:

—CH

2

—,

—CH

2

—CH

2

—,

—CHR

4

—,

R

4

being selected from:

H;

(C

1

-C

20

) alkyl substituted or unsubstituted by one or more of the following groups:

amino, hydroxyl, (C

1

-C

5

)alkyl, (C

1

-C

5

)alkoxy, (C

1

-C

5

)alkylthio, (C

1

-C

5

)alkylamino, trifluoromethyl;

(C

3

-C

8

)cycloalkyl substituted or unsubstituted by one or more of the following groups:

amino, hydroxyl, (C

1

-C

5

) alkyl, (C

1

-C

5

) alkoxy, (C

1

-C

5

)alkylthio, (C

1

-C

5

)alkylamino, trifluoromethyl;

(C

3

-C

8

) heterocycloalkyl containing one or more heteroatoms selected from N, O and S and substituted or unsubstituted by one or more of the following groups:

amino, hydroxyl, (C

1

-C

5

) alkyl, (C

1

-C

5

) alkoxy, (C

1

-C

5

)alkylthio, (C

1

-C

5

) alkylamino, trifluoromethyl; a nitrogen atom can additionally be substituted by a (C

6

-C

14

) aryl, (C

6

-C

14

) acyl or (C

1

-C

5

) alkyl group;

and their solvates and pharmaceutically acceptable salts.

The heteroaryl groups are selected in particular from pyridyl, pyrimidinyl, furyl, pyrrolyl, thienyl, benzothiazolyl, benzoxazolyl, benzimidazolyl, benzothienyl, indolyl, benzofuryl and imidazolyl.

The heterocycloalkyl groups are selected in particular from piperidinyl, morpholinyl, piperazinyl, tetrahydrofuryl, tetrahydrothienyl, pyrrolidinyl and tetrahydropyranyl.

A preferred group of compounds of formula I is constituted in which R

1

is H or (C

1

-C

6

)alkyl.

A preferred group of compounds of formula I is constituted in which:

R

2

is selected from

(C

3

-C

8

) cycloalkyl substitued or unsubstituted by one or more of the following groups: amino, hydroxyl, (C

1

-C

5

) alkyl, (C

1

-C

5

)alkoxy, (C

1

-C

5

)alkylthio, (C

1

-C

5

)alkylamino, trifluoromethyl;

(C

6

-C

14

) aryl substituted or unsubstituted by one or more of the following groups: amino, hydroxyl, halogen, (C

1

-C

5

) alkyl, (C

1

-C

5

)alkoxy, (C

1

-C

5

) alkylthio, (C

1

-C

5

)alkylamino, trifluoromethyl, cyano;

(C

1

-C

13

) heteroaryl containing one or more heteroatoms selected from N, O and S and substituted or unsubstituted by one or more of the following groups: amino, hydroxyl, halogen, (C

1

-C

5

) alkyl, (C

1

-C

5

) alkoxy, (C

1

-C

5

)alkylthio, (C

1

-C

5

) alkylamino, trifluoromethyl, cyano;

a nitrogen atom can additionally be substituted by a (C

6

-C

14

) aryl, (C

6

-C

14

) acyl or (C

1

-C

5

) alkyl group.

A particularly preferred group of compounds of formula I is constituted in which R

2

is selected from a (C

6

-C

14

) aryl group possibly substituted as defined above.

To the knowledge of the Applicant, the compounds of formula (I) are novel with the exception of α-guanidinoacetanilide, which is described by Mazundar (Indian Drugs 1989, 27, 5, 292), i.e. the compound of formula I in which R=H, R

2

=phenyl and R

3

=H.

The invention also relates to the tautomeric forms, to the enantiomers, diastereoisomers and epimers of the compounds of general formula (I).

The compounds of general formula (I) have basic nitrogen atoms and can be monosalified or disalified by mineral or organic acids.

The compounds of formula (I) can be prepared by reacting a compound of formula:

with a compound of formula:

in which:

R

5

is selected from —SCH

3

, pyrazolyl or —SO

3

H, and

T is a tert-butyloxycarbonyl or benzyloxycarbonyl protective group.

The compounds of formula (II) can be prepared in accordance with a process in which:

a) a compound of formula:

in which R

2

and R

3

are as defined above is reacted with an acyl halide of general formula (VI):

in which:

A is as defined above,

L represents a chlorine or bromine atom or an activated ester form,

Z represents a chlorine or bromine atom or a protected amino group, to form a compound of general formula (VII):

in which R

2

, R

3

, A and Z are as defined above;

b) the compound of general formula (VII) is reacted with potassium phthalimide and then with hydrazine monohydrate or else with a nitride followed by a reduction to form a compound of general formula (II) in which R

1

=H.

In the case where Z is a protected amino group, it is appropriate to deprotect it at this stage to form a compound of general formula (II).

The compounds of general formula (II) in which R

1

is other than H can be obtained by reacting a compound of formula (VII) with an amine of general formula (VIII):

R

1

NH

2

(VIII).

The compositions according to the present invention are useful in the treatment of pathologies associated with insulin resistance syndrome (syndrome X).

Insulin resistance is characterized by a reduction in the action of insulin (cf. Presse Medicale, 1997, 26 (No. 14), 671-677) and is involved in a large number of pathological states, such as diabetes and, more particularly, non-insulin-dependent diabetes (type II diabetes or NIDDM), dyslipidaemia, obesity, arterial hypertension, and certain microvascular and macrovascular complications such as atherosclerosis, retinopathies and neuropathies.

In this context, reference may be made, for example, to Diabetes, Vol. 37, 1988, 1595-1607; Journal of Diabetes and its complications, 1998, 12, 110-119, or Horm. Res., 1992, 38, 28-32.

In particular, the compositions of the invention exhibit a strong hypoglycaemic activity.

The compositions according to the present invention can also be used for treating the chronic complications due to the formation of “advanced glycosylation end products”, written AGEs, which result from the glycoxidation reaction between glucose, its oxidation derivatives and the amino functions of proteins, including the so-called Maillard reactions of glycation of glyoxal for example.

The pharmaceutical compositions according to the invention can be provided in forms which are intended for parenteral, oral, rectal, permucous or percutaneous administration.

They will therefore be provided in the form of solutions or suspensions for injection or multi-dose bottles, in the form of plain or coated tablets, film-coated tablets, wafer capsules, gelatin capsules, pills, cachets, powders, suppositories or rectal capsules, or solutions or suspensions for percutaneous use in a polar solvent or for permucous use.

The excipients which are suitable for such administrations are derivatives of cellulose or microcrystalline cellulose, alkaline earth metal carbonates, magnesium phosphate, starches, modified starches, and lactose for the solid forms.

For rectal use, cocoa butter or polyethylene glycol stearates are the preferred excipients.

For parenteral use, water, aqueous solutions, physiological saline and isotonic solutions are the vehicles used most judiciously.

The dosage can vary within wide limits (from 0.5 mg to 1000 mg) depending on the therapeutic indication and the administration route, and also on the age and weight of the individual.

The following examples illustrate the preparation of the compounds of formula (I) and of various intermediates.

A—PREPARATION EXAMPLE OF COMPOUNDS OF FORMULA (VII)

Preparation of N-(2,6-Dimethylphenyl)-3-chloropropanamide

A 2 l three-necked flask, is charged with 121.8 g of 2,6-dimethylaniline, 300 ml of isopropyl ether and 150 ml of water. 126.5 g of 3-chloropropanoyl chloride are added at a rate such that the internal temperature is between 5 and 10° C. At the end of the addition, the reaction medium is stirred for 3 h. The precipitate formed is filtered off with suction, washed with isopropyl ether and then with water and finally dried. 126 g of white crystals are obtained.

m.p.=134-136° C.

1

H NMR (DMSO-d6, 200 MHz): 2.18 (s,6H); 2.81 (t,2H); 3.88 (t,2H); 7.03 (d,3H); 9.40 (s,1H).

The formulae and characteristics of compounds of formula (VII) have been collated in Table 1.

TABLE 1

m.p. in ° C.

1

H NMR

Compound

Structure

(Köfler)

200 MHz d ppm

1

146-148 lit.: 146-148

DMSO-d6 2.25 (s,6H) 4.35 (s,2H) 9.10 (s,3H) 9.62 (s,1H)

2

148-150 lit.: 151.4-151.9

cDC13 1.20 (d,12H) 3.00 (m,2H) 4.20 (s,2H) 7.20 (m,3H) 7.82 (s,1H)

3

197-199 lit.: 190-192

DMSO-d6 2.65 (2s,9H) 4.50 (s,2H) 7.13 (s,1H) 10.06 (s,1H)

4

134-136 lit.: 134-136

DMSO-d6 2.18 (s,6H) 2.81 (t,2H) 3.88 (t,2H) 7.03 (d,3H) 9.40 (s,1H)

m.p. in ° C.

1

H NMR

Compound

Structure

(Köfler)

200 MHz δ ppm

5

176-178

DMSO-d6 1.23 (d,3H) 2 (s,6H) 4.15 (m,1H) 4.90 (s,2H) 6.90 (s,3H) 7.23 (s,5H) 7.45 (d,1H) 9.20 (s,1H)

6

184-186

DMSO-d6 2.20 (s,6H) 2.40 (s,3H) 4.35 (s,2H) 7.00 (s,2H) 9.62 (s,1H)

7

195-197

DMSO-d6 1.0 (t,3H) 1.18 (d,3H) 1.25-2.00 (m,3H) 2.30 (s,6H) 4.20 (t,1H) 7.20 (s,3H) 7.50 (s,5H) 7.70 (d,2H) 9.55 (s,1H)

8

oil

DMSO-d6 1.0 (t,6H) 1.69 (m,3H) 2.18 (s,6H) 4.30 (q,1H) 5.09 (q,2H) 7.09 (s,3H) 7.40 (s,5H) 7.63 (d,1H) 9.40 (s,1H)

9

210-212

DMSO-d6 1.0 (t,6H) 1.70 (m,1H) 2.13 (s,6H) 4.10 (t,1H) 5.11 (q,2H) 7.05 (s,3H) 7.36 (s,5H) 7.50 (d,1H) 9.40 (s,1H)

10

oil

DMSO-d6 4.30 (s,4H) 4.40 (s,2H) 7.02 (m,10H)

11

172-174

DMSO-d6 4.33 (s,2H) 7.42 (m,3H) 8.21 (s,1H)

12

114-116

DMSO-d6 1.39 (m,5H) 1.80 (m,4H) 3.66 (s,2H) 4.16 (s,2H) 8.25 (d,1H)

13

133-135

DMSO-d6 3.99 (s,2H) 4.16 (d,2H) 7.16 (m,5H) 8.62 (s,1H)

B—PREPARATION EXAMPLE OF COMPOUNDS OF FORMULA (II)

Preparation of N-(2,6-Dimethylphenyl)-3-aminopropanamide

A three-necked flask is charged with 400 ml of DMF, 100 g of N-(2,6-dimethylphenyl)-3-chloropropanamide and 95.7 g of potassium phthalimide. After 3 h of stirring at reflux, the reaction medium is cooled to room temperature and 600 ml of water are added. Stirring is continued for 1.5 h. The precipitate formed is filtered off with suction and washed with water. 140 g of white crystals (m.p.>260° C.) are obtained.

The crystals are charged to a three-necked flask with 800 ml of 95° ethanol and 23.7 g of hydrazine monohydrate. After 3 h of stirring at reflux, 39 ml of concentrated hydrochloric acid are added and stirring is continued for 1 h. The precipitate formed is filtered off, and washed with ethanol and the ethanolic phases are concentrated. The crude product obtained is taken up in water; the insoluble material remaining is filtered off and the aqueous phase is concentrated to give 108 g of a cream-white solid.

m.p.=239-241° C.

1

H NMR (DMSO-d6, 200 MHz): 2.22 (s,6H); 2.44 (t,2H); 2.88 (t,2H); 7.07 (s,3H); 9.50 (s,1H).

The formulae and characteristics of compounds of formula (II) have been collated in Table 2.

TABLE 2

m.p. in ° C.

1

H NMR

Compound

Structure

(Köfler)

200 MHz d ppm

1

>260 (HCl) lit.: 296

DMSO-d6 2.15 (d,6H) 3.90 (d,2H) 7.12 (s,3H) 8.40 (s,3H) 10.15 (s,1H)

2

118-120

DMSO-d6 0.88 (s,12H) 2.77 (m,2H) 3.11 (s,2H) 6.92 (m,3H) 9.07 (s,1H)

3

132-134

DMSO-d6 2.40 (s,6H) 2.48 (s,3H) 3.34 (s,3H) 4.26 (s,3H) 6.90 (s,1H)

4

239-241 (HCl) lit.: 239-240

DMSO-d6 2.22 (s,6H) 2.44 (t,2H) 2.88 (t,2H) 7.07 (s,3H) 9.50 (s,1H)

m.p. in ° C.

1

H NMR

Compound

Structure

(Köfler)

200 MHz δ ppm

5

73-75

DMSO-d6 1.25 (d,3H) 2.13 (s,6H) 3.50 (m,3H) 7.00 (s,3H)

6

119-121

DMSO-d6 2.09 (s,6H) 2.18 (s,3H) 3.28 (s,2H) 6.87 (s,2H)

7

74-76

DMSO-d6 1.04 (t,3H) 1.20 (d,3H) 1.30 (m,1H) 1.78 (m,3H) 2.25 (s,6H) 3.35 (d,1H) 7.13 (s,3H) 9.40 (s,1H)

8

oil

DMSO-d6 1.0 (t,6H) 1.63 (m,3H) 2.21 (s,6H) 3.45 (m,1H) 7.12 (s,3H)

9

71-73

DMSO-d6 0.90 (d,3H) 1.05 (d,3H) 2.04 (m,1H) 2.25 (s,6H) 3.27 (d,1H) 7.03 (s,3H)

10

oil

DMSO-d6 3.00 (s,2H) 3.54 (s,2H) 4.54 (d,4H) 7.33 (n,10H)

11

108-110

DMSO-d6 3.57 (s,2H) 4.69 (s,3H) 7.51 (t,1H) 7.75 (d,2H)

12

89-91

DMSO-d6 1.47 (t,5H) 1.91 (m,5H) 3.24 (s,2H) 3.77 (m,1H) 7.83 (d,1H)

13

oil

DMSO-d6 2.69 (s,2H) 3.34 (s,2H) 4.50 (d,2H) 7.49 (m,5H) 8.52 (s,1H)

C—PREPARATION EXAMPLE OF COMPOUNDS OF FORMULA (II)

Preparation of N-(2,6-Dimethylphenyl)-2-methylamino-acetamide

A closed stainless-steel reactor is charged with 80 g of N-(2,6-dimethylphenyl)-2-chloro-acetamide and 600 ml of a 40% aqueous methylamine solution. The mixture is heated with stirring at 80° C. for 4 h and then concentrated under vacuum. The crude product is taken up in water and washed with dichloromethane. The aqueous solution is basified using sodium hydroxide solution and extracted with dichloromethane. The organic, phase is dried over sodium sulphate and concentrated to give 60 g of a colourless oil.

1

H NMR (DMSO-d6, 200 MHz): 2.15 (s,6H); 2.44 (s,3H); 3.50 (s,2H); 6.50 (s,1H); 7.70 (s,3H); 9.58 (s,1H).

The formulae and characteristics of compounds have been collated in Table 3.

TABLE 3

m.p. in ° C.

1

H NMR

Compound

Structure

(Köfler)

200 MHz δ ppm

1

oil

DMSO-d6 2.15 (s,6H) 2.44 (s,3H) 3.50 (s,2H) 6.50 (s,1H) 7.07 (s,3H) 9.58 (s,1H)

2

oil

13C NMR 50 MHz δ ppm DMSO-d6 34.80 CH3-N 54.28 CH2 180.09 C═O

D—PREPARATION EXAMPLE OF COMPOUNDS OF FORMULA (I)

Preparation of 2-Aminoiminomethylamino-N-(cyclohexyl)acetamide

A round-bottomed flask is charged with 15 g of 2-amino-N-(cyclohexyl)-acetamide, 14,6 g 1-aminoiminomehtylpyrazole hydrochloride and 100 ml of dioxane. After 18 h stirring at reflux, the reaction medium is cooled to room temperature and precipitate which has formed is filtered off with suction. 16 g of a white solid are obtained.

m.p.=232-234° C.

1

H NMR (DMSO-d6, 200 MHz): 1.19 (m,5H); 1.62 (m,5H); 3.46 (m,1H); 3.78 (d,2H); 7.21 (s,4H); 7.68.

The formulae and characteristics of compounds of formula (I) have been collated in Table 4.

TABLE 4

m.p. in ° C.

1

H NMR

Compound

Structure

(Köfler)

200 MHz d ppm

1

255-257 (0.5 H

2

SO

4

DMSO-d6 2.25 (s,6H) 4.25 (s,2H) 7.15 (s,3H) 7.95 (s,3H) 8.35 (s,1H) 10.10 (s,1H)

2

260-262 (HCl)

DMSO-d6 1.31 (d,12H) 3.22 (m,2H) 4.35 (s,2H) 7.37 (m,3H) 7.64 (s,2H) 7.91 (s,2H) 9.91 (s,1H)

3

249-251 (HCl)

DMSO-d6 2.40 (3s,9H) 4.15 (s,2H) 6.92 (s,1H) 7.44 (s,3H) 7.74 (s,1H) 9.88 (s,1H)

4

223-225

DMSO-d6 2.16 (s,6H) 2.65 (t,2H) 3.50 (t,2H) 7.08 (s,3H) 7.31 (m,3H) 7.85 (s,2H) 9.69 (s,1H)

5

227-229 (HCl)

DMSO-d6 2.05 (s,6H) 2.88 (s,3H) 4.25 (s,2H) 6.95 (s,4H) 9.68 (s,1H)

m.p. in ° C.

1

H NMR

Compound

Structure

(Köfler)

200 MHz δ ppm

6

159-161 (HCl)

DMSO-d6 1.53 (d,3H) 2.27 (s,6H) 7.20 (s,3H) 4.85 (m,1H) 7.20 (d,1H) 7.55 (s,2H) 8.10 (d,1H) 10.00 (s,1H)

7

>260 (HCl)

DMSO-d6 2.15 (s,6H) 2.24 (s,3H) 4.18 (d,2H) 6.90 (s,2H) 7.50 (s,2H) 7.87 (s,1H) 9.70 (s,1H)

8

amorphous (HCl)

DMSO-d6 1.0 (m,6H) 1.30 (m,1H) 1.50 (m,1H) 1.93 (m,H) 2.25 (s,6H) 4.60 (d,1H) 7.17 (s,3H) 7.95 (s,2H) 9.55 (s,1H)

9

amorphous (HCl)

DMSO-d6 1.05 (d,6H) 1.69 (m,3H) 2.18 (s,6H) 4.87 (s,1H) 7.15 (s,3H) 8.02 (s,2H) 10.18 (s,1H)

10

amorphous (HCl)

DMSO-d6 1.0 (m,6H) 2.25 (m,7H) 4.57 (t,1H) 7.12 (s,3H) 8.08 (s,2H) 10.20 (s,1H)

11

217-219 (HCl)

DMSO-d6 4.51 (s,2H) 4.68 (s,2H) 7.58 (m,15H)

12

181-183 (HCl)

DMSO-d6 4.30 (d,2H) 7.52 (m,3H) 7.91 (s,1H) 10.45 (s,1H)

13

>250 (HCl)

DMSO-d6 3.00 (s,3H) 3.52 (s,5H) 3.76 (s,2H)

14

232-234 (HCl)

DMSO-d6 1.19 (m,5H) 1.62 (m,5H) 3.46 (m,1H) 3.78 (d,2H) 7.21 (s,4H) 7.68 (t,1H) 8.16 (d,1H)

15

144-146 (HCl)

DMSO-d6 4.04 (d,2H) 4.42 (d,2H) 7.44 (m,9H) 7.79 (t,1H) 8.89 (t,1H)

The results of pharmacological studies will be given below.

Study of the Antidiabetic Activity in the Nostz Rat

The antidiabetic activity of the compounds of formula (I) was determined orally on an experimental model of non-insulin-dependent diabetes induced in the rat using streptozotocin.

The model of non-insulin-dependent diabetes is obtained in rats by neonatal injection (on the day of birth) of streptozotocin.

The diabetic rats used are 8 weeks old. The animals are kept from the day of their birth to the day of the experiment in an animal house at a regulated temperature of from 21 to 22° C. and are subjected to a fixed cycle of light (from 7.00 am to 7.00 pm) and darkness (from 7.00 pm to 7.00 am). Their diet consisted of a maintenance diet; water and food were provided ad libitum, except for the 2 hours fasting prior to the tests in which food is withdrawn (post-absorptive state).

The rats are treated orally for one (d1) or four (d4) days with the test product. Two hours after the final administration of the product and 30 minutes after anaesthesia of the animals with sodium pentobarbital (Nembutalo®), 300 ml of blood is sampled from the end of the tail.

Results obtained are collated, by way of example, in Table 5. These results demonstrate the efficacy of the compounds of formula (I) in reducing glycaemia in diabetic animals. These results are expressed as a percentage change in glycaemia on d1 and d4 (number of days of treatment) relative to d0 (before treatment).

TABLE 5

20 mg/kg/d

200 mg/kg/d

d1

d4

d1

d4

Example

1

−3

−7

−20

−24

3

+7

−7

−5

−4

4

−1

−11

−13

−15

5

−2

−7

−16

−25

Compounds

6

+8

−3

−4

−18

7

−9

−9

−3

−10

8

−11

−14

−8

−10

9

−4

−15

+7

−4

10

−13

−15

−3

−16

11

−14

−9

−3

−16

12

−9

−13

−14

−27

Study of the Antiglycoxidation Activity

The compounds (I) are also capable of inhibiting the so-called Maillard reactions by means of a “scavenging” effect on α-dicarbonyl derivatives such as glyoxal—this is the antiglycation effect. This inhibitory effect on the Maillard reaction by the compounds of formula (I) was studied in vitro by assaying the ketamines (“fructoamines”) produced during the incubation of albumin with methylglyoxal in the presence or absence of a compound of formula (I).

A 6.6 mg/ml solution of bovine albumin in 0.2 M phosphate buffer pH 7.4 is incubated with 1 mM methylglyoxal in the presence or absence of a compound according to the invention at a concentration of 1 mM. The incubation is carried out under sterile conditions at 37° C. for 6 days. At the end of the incubation period, the amount of ketamines is measured with a commercially available fructoamine assay kit (kit “FRA”, product reference: 0757055, Roche S. A. products) in accordance with the manufacturer's instructions. Under these experimental conditions, the level of fructoamine following incubation of albumin with methylglyoxal in the presence of a compound of formula (i) is from 30 to 50% lower than that observed when albumin is incubated with methylglyoxal in the absence of the compound of formula (I).

Claims

1. A compound which is
2. A pharmacuetical composition comprising a compound according to claim 1 and a pharmacuetical acceptable excipient.
3. A method for treating a pathology associated with insulin resistance syndrome, comprising administering to a patient in need thereof an effective amount of a composition of claim 2.
4. A process for preparing a compound of formula (I): in which:R1 is H or a C1-C20 alkyl which is unsubstituted or substituted by one or more amino, hydroxyl, C1-C5 alkyl, C1-C5 alkoxy, C1-C5 alkylthio, C1-C5 alkylamino or trifluoromethyl groups, R is a C3-C8 cycloalkyl which is unsubstituted or substituted by one or more amino, hydroxyl, C1-C5 alkyl, C1-C5 alkoxy, C1-C5 alkylthio, C1-C5 alkylamino or trifluoromethyl groups, a C6-C14 aryl which is unsubstituted or substituted by one or more amino, hydroxyl, halogen, C1-C5 alkyl, C1-C5 alkoxy, C1-C5 alkylthio, C1-C5 alkylamino, trifluoromethyl or cyano groups, or a C1-C13 heteroaryl which contains one or more heteroatoms selected from the group consisting of N, O and S, and which is unsubstituted or substituted by one or more amino, hydroxyl, halogen, C1-C5 alkyl, C1-C5 alkoxy, C1-C5 alkylthio, C1-C5 alkylamino, trifluoromethyl or cyano groups, wherein a nitrogen atom is optionally substituted with a C6-C14 aryl, C6-C14 acyl or C1-C5 alkyl group, R3 is H, a C3-C8 cycloalkyl which is unsubstituted or substituted by one or more amino, hydroxyl, C1-C5 alkyl, C1-C5 alkoxy, C1-C5 alkylthio, C1-C5 alkylamino or trifluoromethyl groups, a C3-C8 heterocycloalkyl which contains one or more heteroatoms selected from the group consisting of N, O and S, and which is unsubstituted or substituted by one or more amino, hydroxyl, C1-C5 alkyl, C1-C5 alkoxy, C1-C5 alkylthio, C1-C5 alkylamino or trifluoromethyl groups, wherein a nitrogen atom is optionally substituted with a C6-C14 aryl, C6-C14 acyl or C1-C5 alkyl group, a C6-C14 aryl which is unsubstituted or substituted by one or more amino, hydroxyl, halogen, C1-C5 alkyl, C1-C5 alkoxy, C1-C5 alkylthio, C1-C5 alkylamino, trifluoromethyl or cyano groups, a C1-C13 heteroaryl which contains one or more heteroatoms selected from the group consisting of N, O and S, and which is unsubstituted or substituted by one or more amino, hydroxyl, C1-C5 alkyl, C1-C5 alkoxy, C1-C5 alkylthio, C1-C5 alkylamino, trifluoromethyl or cyano groups, wherein a nitrogen atom is optionally substituted with a C6-C14 aryl, C6-C14 acyl or C1-C5 alkyl group, or a C6-C14 aryl-C1-C5 alkyl which is unsubstituted or substituted by one or more amino, hydroxyl, C1-C5 alkyl, C1-C5 alkoxy, C1-C5 alkylthio, C1-C5 alkylamino, trifluoromethyl or cyano groups, and A is —CH2—CH2, or —CHR4—, wherein R4 is H, a C1-C20 alkyl which is unsubstituted or substituted by one or more amino, hydroxyl, C1-C5 alkyl, C1-C5 alkoxy, C1-C5 alkylthio, C1-C5 alkylamino or trifluoromethyl groups, a C3-C8 cycloalkyl which is unsubstituted or substituted by one or more amino, hydroxyl, C1-C5 alkyl, C1-C5 alkoxy, C1-C5 alkylthio, C1-C5 alkylamino or trifluoromethyl groups, a C3-C8 heterocycloalkyl which contains one or more heteroatoms selected from the group consisting of N, O and S, and which is unsubstituted or substituted by one or more amino, hydroxyl, C1-C5 alkyl, C1-C5 alkoxy, C1-C5 alkylthio, C1-C5 alkylamino or trifluoromethyl groups, wherein a nitrogen atom is optionally substituted with a C6-C14 aryl, C6-C14 acyl or C1-C5 alkyl group; or a solvate or a pharmaceutically acceptable salt thereof, with the exception of the compound of formula (I) in which R1=H, R2=phenyl and R3=H, comprising reacting a compound of formula (U) with a compound of formula (II) or (IV) in which R5 is —SCH3, pyrazolyl —SOH3H, and T is a tert-butyloxycarbonyl or benzyloxycarbonyl protective group.

Priority Claims (1)

Number	Date	Country	Kind
99 00194	Jan 1999	FR

PCT Information

Filing Document	Filing Date	Country	Kind
PCT/EP99/10468		WO	00

Publishing Document	Publishing Date	Country	Kind
WO00/42001	7/20/2000	WO	A

US Referenced Citations (1)

Number	Name	Date	Kind
6147258	Greindl et al.	Nov 2000	A

Foreign Referenced Citations (1)

Number	Date	Country
1153424	May 1969	GB

Non-Patent Literature Citations (5)

Entry
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Guillou Y Et Al: “Phascoline[377N-(3-guanidinopropionyl)-2-hyd roxy-n-heptylamine 377]and phascolosomine [377N-(3-guanidinoisobutyryl)-2-methoxy-n-heptylamine 377], two new guanidino compounds from sipunculid worms. Isolation and structure” J. Biol. Chem. (JBCHA3); 1973; vol.248 (16); pp. 5668-72, XP002117857 Coll. France;Ec. Prat. Hautes Etud.; Paris; Fr.
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(Aminoiminomethyl) amino) alkane-carboxamides and their applications in therapy

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