AMINOPYRAZOLE AMIDE DERIVATIVE

TECHNICAL FIELD

The invention relates to a pyrazole amide compound useful as a medicament. More specifically, the invention relates to a therapeutic or preventive agent for conditions related to glucocorticoid, or a pyrazole amide compound which is effective as an 11β hydroxysteroid dehydrogenase type 1 enzyme (referred to as 11βHSD1 hereinafter) inhibitor or 11βHSD1 modulator. The invention further relates to a therapeutic agent for diabetes that the active ingredient is a pyrazole amide compound which is effective as an 11βHSD1 inhibitor or 11βHSD1 modulator.

BACKGROUND ART

Glucocorticoid regulates peripheral glucose metabolism and amino acid metabolism. In human being, glucocorticoid is produced in adrenal gland and is metabolized in peripheral tissues including adipose tissue or liver. Since 11βHSD1 is an enzyme converting inactive cortisone into activated cortisol and is mainly expressed in adipose tissue or liver, 11βHSD1 is believed to be related to glucocorticoid activation in adipose tissue or liver. Cortisol shows promoting activities for fat accumulation in adipocyte or gluconeogenesis in liver, and hence, 11βHSD1 is believed to contribute to the maintenance of systemic homeostasis by adjusting glucose and/or lipid metabolism in periphery. On the other hand, in human insulin resistance patients, 11βHSD1 in adipose tissues was significantly increased in the activity, and the 11βHSD1 activity in visceral fat is remarkably higher than that in subcutaneous fat. In 11βHSD1 gene defect mice, development of visceral fat accumulation, glucose and/or lipid metabolism abnormality is suppressed on high-fat food feeding, and mice overexpressing adipocyte-specific 11βHSD1 show remarkable visceral fat-type obesity, or glucose and/or lipid metabolism abnormality. This indicates that an overactivation of 11βHSD1 is intimately related to development of visceral fat accumulation and/or metabolic syndrome in both human and mice. Specifically, advantageous effects including suppression of gluconeogenesis in liver and fat accumulation in adipocyte as well as improvement of systemic glucose and/or lipid metabolism are expected by inhibiting the enzyme activity.

As far the improvement of glucose metabolism, it has been reported that the 11βHSD1 activity in pancreatic β cells could relate to the suppression of insulin secretion and the 11βHSD1 activity could be involved in the suppression of glucose uptake in human muscle cells. Thus, an 11βHSD1 inhibitor has potential to improve hyperglycemia directly.

Additionally, 11βHSD1 has been shown to function in nerve cells or immunocytes, and the 11βHSD1 inhibitor is also expected to have therapeutic effects on diseases caused by the above abnormalities.

Various 11βHSD1 inhibitors have been reported. For example, it is reported that derivatives with pyrazole ring in Patent Document 1, and amide derivatives in Patent Document 2 are effective as 11βHSD1 inhibitor.

[Patent Document 1] WO2005/016877 pamphlet

[Patent Document 2] WO2004/089470 pamphlet

DISCLOSURE OF INVENTION
Problems to be Resolved by the Invention

A development of a pharmaceutically satisfiable compound which shows 11βHSD1 inhibitory effect as a therapeutic agent for preventing and/or treating diseases, including type II diabetes, abnormal glucose tolerance, hyperglycemia, insulin resistance, hypo-HDL-emia, hyper-LDL-emia, dyslipidemia, hyperlipidemia, hypertriglyceridemia, hypercholesterolemia, hypertension, arteriosclerosis, angiostenosis, atherosclerosis, obesity, cognitive disorder, glaucoma, retinopathy, dementia, Alzheimer disease, osteoporosis, immune disorder, syndrome X, depression, cardiovascular disease, neurodegenerative disease, has now been desired.

Means of Solving the Problems

Until now, the following [1] pyrazole-3-carboxylic acid amide derivatives of formula (1) has not been prepared for 11βHSD1 inhibitor, and the inhibitory activity thereof has been completely unknown. As a result of extensive studies of the derivatives in order to achieve the subject, the inventors have found that pyrazole-3-carboxylic acid amide derivatives of formula (1), which are substituted with alkyl etc. at 1-position and dialkylamino etc. at 5-position, have high 11βHSD1 inhibitory activity.

The inventors have found that pyrazole-3-carboxylic acid amide derivatives of formula (1) or pharmaceutically acceptable salts thereof, if needed, which are referred to as inventive compounds hereinafter, have an excellent 11βHSD1 inhibitory activity, and have achieved this invention.

Specifically, the invention relates to the following embodiments:

[1] A compound of formula (1):

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wherein R_Aand R_Bare each independently optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, or a group of formula: —Rw—Rx—Ry—Rz;

Rw is, independently when it exists more than one, optionally substituted alkylene or optionally substituted cycloalkylene;

Rx is, independently when it exists more than one, a single bond, oxygen atom, or a group of formula: —S(O)_n—, —C(O)—, —NR³—, —OC(O)—, —C(O)O—, —CONR³—, —NR³CO—, —SO₂NR³—, —NR³SO₂— or —NR³CONR⁴—;

Ry is, independently when it exists more than one, a single bond or optionally substituted alkylene;

Rz is, independently when it exists more than one, optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted aryl, optionally substituted heteroaryl or optionally substituted heterocycloalkyl;

R³and R⁴are each independently hydrogen atom or optionally substituted alkyl;

n is 0, 1 or 2;

R_Cis optionally substituted alkyl, optionally substituted cycloalkyl or optionally substituted cycloalkylalkyl;

R_Dis hydrogen atom, halogen atom, cyano or optionally substituted alkyl;

R_Eis hydrogen atom or optionally substituted alkyl;

R_Fis a group selected from the following formulae (G1):

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wherein one of hydrogen atoms is a bond, which may be optionally substituted;

provided that if both R_Aand R_Bare selected from the following group X, then R_Fis a group of the following formula (2):

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A₁is COOR¹, CONR¹R², SO₂NR¹R², COOR¹-substituted alkyl, CONR¹R²-substituted alkyl, or SO₂NR¹R²-substituted alkyl, R¹and R²are each independently hydrogen atom or optionally substituted alkyl, or R¹and R²may combine each other together with the adjacent nitrogen atom to form optionally substituted saturated heterocycle;

the group X is optionally substituted alkyl, optionally substituted piperidinyl, optionally substituted pyrrolidinyl, optionally substituted arylalkyl, optionally substituted heteroarylalkyl, optionally substituted piperidinylalkyl or optionally substituted pyrrolidinylalkyl, wherein the substituent is hydroxyl, oxo, halogen atom, cyano, nitro, alkyl, alkoxy, amino which may be optionally substituted by alkyl or arylalkyl, methylenedioxy, trihalomethyl, or trihalomethoxy; or a pharmaceutically acceptable salt thereof;

[2] The compound of [1] of formula (3):

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wherein R_Aand R_Bare each independently optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, or a group of formula: —Rw—Rx—Ry—Rz;

Rw is, independently when it exists more than one, optionally substituted alkylene or optionally substituted cycloalkylene;

Ry is, independently when it exists more than one, a single bond or optionally substituted alkylene;

R³and R⁴are each independently hydrogen atom or optionally substituted alkyl;

n is 0, 1 or 2;

R_Cis optionally substituted alkyl, optionally substituted cycloalkyl or optionally substituted cycloalkylalkyl;

R_Dis hydrogen atom, halogen atom, cyano or optionally substituted alkyl;

R_Eis hydrogen atom or optionally substituted alkyl;

A is hydrogen atom, halogen atom, hydroxyl, cyano, or a group of formula: COOR¹, CONR¹R², SO₂NR¹R², COOR¹-substituted alkyl, CONR¹R²-substituted alkyl, or SO₂NR¹R²-substituted alkyl, R¹and R²are each independently hydrogen atom or optionally substituted alkyl, or R¹and R²may combine each other together with the adjacent nitrogen atom to form optionally substituted saturated heterocycle;

provided that if both R_Aand R_Bare selected from the following group X, then A is COOR¹, CONR¹R², SO₂NR¹R², COOR¹-substituted alkyl, CONR¹R²-substituted alkyl, or SO₂NR¹R²-substituted alkyl;

the group X is optionally substituted alkyl, optionally substituted piperidinyl, optionally substituted pyrrolidinyl, optionally substituted arylalkyl, optionally substituted heteroarylalkyl, optionally substituted piperidinylalkyl, or optionally substituted pyrrolidinylalkyl, wherein the substituent is hydroxyl, oxo, halogen atom, cyano, nitro, alkyl, alkoxy, amino which may be optionally substituted by alkyl or arylalkyl, methylenedioxy, trihalomethyl, or trihalomethoxy; or a pharmaceutically acceptable salt thereof;

[3] The compound of [2], wherein R_Cis optionally substituted alkyl, R_Dis hydrogen atom, halogen atom or optionally substituted alkyl, R_Eis hydrogen atom, A is halogen atom, hydroxyl, cyano, or a group of formula: COOR¹, CONR¹R², SO₂NR¹R², COOR¹-substituted alkyl, CONR¹R²-substituted alkyl or SO₂NR¹R²-substituted alkyl, R¹and R²are each independently hydrogen atom or optionally substituted alkyl, or R¹and R²may combine each other together with the adjacent nitrogen atom to form optionally substituted saturated heterocycle, or a pharmaceutically acceptable salt thereof;

[4] The compound of either [2] or [3], wherein R_Aand R_Bare each independently optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted cycloalkylalkyl or optionally substituted heterocycloalkyl, A is a group of formula: COOR¹, CONR¹R²or SO₂NR¹R², R¹and R²are each independently hydrogen atom or optionally substituted alkyl, R_Ais optionally substituted cycloalkyl or optionally substituted cycloalkylalkyl, R_Bis optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, or a group of formula: —Rw— Rx—Ry—Rz wherein Rw, Rx, Ry and Rz are the same as defined in [2]; or R_Ais optionally substituted alkyl, R_Bis a group of formula: —Rw—Rx—Ry—Rz wherein Rw, Rx, Ry and Rz are the same as defined in [2], or a pharmaceutically acceptable salt thereof;

[5] The compound of any one of [2] to [4], wherein R_Aand R_Bare each independently optionally substituted alkyl, optionally substituted cycloalkyl or optionally substituted cycloalkylalkyl,

A is a group of formula: COOR¹, CONR¹R²or SO₂NR¹R², R¹and R²are each independently hydrogen atom or optionally substituted alkyl, or a pharmaceutically acceptable salt thereof;

[6] The compound of either [4] or [5], wherein A is a group of formula: CONR¹R², R¹and R²are each independently hydrogen atom or alkyl which may be optionally substituted by hydroxyl, alkoxy, benzenesulfonyl or pyridyl, or a pharmaceutically acceptable salt thereof;

[7] The compound of [6], wherein A and nitrogen atom on which adamantyl group is substituted are arranged in E-configuration, or a pharmaceutically acceptable salt thereof;

[8] The compound of any one of [2] to [4], wherein R_Ais optionally substituted cycloalkyl or optionally substituted cycloalkylalkyl, R_Bis optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, or a group of formula: —Rw—Rx—Ry—Rz wherein Rw, Rx, Ry and Rz are the same as defined in [2], or a pharmaceutically acceptable salt thereof;

[9] The compound of [8], wherein R_Bis optionally substituted alkyl, optionally substituted heterocycloalkyl, or a group of formula: —Rw—Rx—Ry—Rz wherein Rw is optionally substituted alkylene,

Rx is a single bond, oxygen atom, or a group of formula: —S(O)_n—, Ry is a single bond, Rz is optionally substituted aryl or optionally substituted heterocycloalkyl, or a pharmaceutically acceptable salt thereof;

[10] The compound of any one of [2] to [4], wherein R_Ais optionally substituted alkyl, R_Bis a group of formula: —Rw—Rx—Ry—Rz wherein Rw, Rx, Ry and Rz are the same as defined in [2], or a pharmaceutically acceptable salt thereof;

[11] The compound of [10], wherein Rx is a group of formula: —S(O)_n—, —C(O)—, —NR³—, —OC(O)—, —C(O)O—, —CONR³—, —NR³CO—, —SO₂NR³—, —NR³SO₂— or —NR³CONR⁴—, R³and R⁴are each independently hydrogen atom or optionally substituted alkyl, n is 0, 1 or 2, or a pharmaceutically acceptable salt thereof;

[12] The compound of [11], wherein Rw is optionally substituted alkylene, Rx is a group of formula: —S(O)_n—, Ry is a single bond, Rz is optionally substituted alkyl, or a pharmaceutically acceptable salt thereof;

[13] The compound of [10], wherein Rx is oxygen atom, Rz is optionally substituted cycloalkyl, optionally substituted aryl, optionally substituted heteroaryl or optionally substituted heterocycloalkyl, or a pharmaceutically acceptable salt thereof;

[14] The compound of [13], wherein Rw is optionally substituted alkylene, Ry is a single bond, Rz is optionally substituted aryl or optionally substituted heterocycloalkyl, or a pharmaceutically acceptable salt thereof;

[15] The compound of [10], wherein Rx is a single bond, Rz is optionally substituted cycloalkyl or optionally substituted heterocycloalkyl, or a pharmaceutically acceptable salt thereof;

[16] The compound of [15], wherein Rw is optionally substituted alkylene, Ry is a single bond, Rz is optionally substituted cycloalkyl or optionally substituted heterocycloalkyl, or a pharmaceutically acceptable salt thereof;

[17] The compound of [10], wherein Rx is a single bond, Rz is substituted aryl, substituted heteroaryl or substituted heterocycloalkyl, in which the substituent is —COR⁵, —S(O)_nR⁵, —NR^7aCOR⁵, —SO₂NR^7aR^7b, —NR^7aCONR^7bR⁵, —OR⁶or —(CH₂)_mR⁶, R⁵is alkyl, cycloalkyl, aryl, heteroaryl or heterocycloalkyl, R⁶is cycloalkyl, aryl, heteroaryl or heterocycloalkyl, the alkyl, cycloalkyl, aryl, heteroaryl and heterocycloalkyl groups in R⁵and R⁶may be further optionally substituted by halogen cycloalkylsulfonyl, alkoxyalkoxy, hydroxyalkoxy, cycloalkyloxyalkyl, cycloalkyloxy, haloalkoxyalkyl, hydroxyalkyl, alkoxyalkyl, NR^8aR^8b-substituted alkyl, alkylsulfonylalkyl, cyanoalkyl, cycloalkylalkyl, cycloalkylsulfonylalkyl, alkoxyalkoxyalkyl, hydroxyalkoxyalkyl or nitrogen-containing saturated heterocycle, R^7a, R^7b, R^8aand R^8bare each independently hydrogen atom or alkyl, n and m are each independently 0, 1 or 2, or a pharmaceutically acceptable salt thereof;

[18] The compound of [17], wherein Rw is optionally substituted alkylene, Ry is a single bond, Rz is substituted aryl or substituted heterocycloalkyl, in which the substituent is —COR⁵or —S(O)_nR⁵, or a pharmaceutically acceptable salt thereof; [19] The compound of [10], wherein Rw is optionally substituted cycloalkylene, Rx is a single bond, Ry is a single bond, Rz is optionally substituted aryl, or a pharmaceutically acceptable salt thereof;

[20] The compound of [2], wherein R_Ais tetrahydropyranyl, R_Bis alkyl or cycloalkyl, or a pharmaceutically acceptable salt thereof;

[21] The compound of [2] of formula (4):

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wherein p is 0, 1 or 2, q is 1 or 2, B¹is a single bond, carbonyl or sulfonyl, B²is optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, optionally substituted alkylamino, optionally substituted dialkylamino, optionally substituted cycloalkylamino, optionally substituted heterocycloalkylamino, optionally substituted arylamino or optionally substituted heteroarylamino, provided that B¹is a single bond, then B²is optionally substituted aryl or optionally substituted heteroaryl, or a pharmaceutically acceptable salt thereof;

[22] The compound of [21], wherein B¹is a single bond, B²is optionally substituted aryl or optionally substituted heteroaryl, or a pharmaceutically acceptable salt thereof;

[23] The compound of [22], wherein B²is optionally substituted aryl, or a pharmaceutically acceptable salt thereof;

[24] The compound of [22], wherein B²is optionally substituted heteroaryl, or a pharmaceutically acceptable salt thereof;

[25] The compound of [24], wherein B²is optionally substituted pyridyl, or a pharmaceutically acceptable salt thereof;

[26] The compound of [21], wherein B¹is carbonyl, B²is optionally substituted aryl, optionally substituted alkyl, optionally substituted cycloalkyl or optionally substituted heteroaryl, or a pharmaceutically acceptable salt thereof;

[27] The compound of [26], wherein the optionally substituted alkyl group in B²is optionally substituted benzyl, or a pharmaceutically acceptable salt thereof;

[28] The compound of [26], wherein the optionally substituted cycloalkyl group in B²is cyclopropyl or cyclobutyl substituted by optionally substituted aryl, or a pharmaceutically acceptable salt thereof;

[29] The compound of [26], wherein B²is optionally substituted aryl, or a pharmaceutically acceptable salt thereof;

[30] The compound of [26], wherein B²is optionally substituted heteroaryl, or a pharmaceutically acceptable salt thereof;

[31] The compound of [26], wherein the optionally substituted heteroaryl group in B²is optionally substituted pyridyl, or a pharmaceutically acceptable salt thereof;

[32] The compound of [26], wherein B²is fluorine-substituted alkyl, or a pharmaceutically acceptable salt thereof;

[33] The compound of [21], wherein B¹is sulfonyl, B²is optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl or optionally substituted heteroaryl, or a pharmaceutically acceptable salt thereof;

[34] The compound of [33], wherein B²is optionally substituted aryl, or a pharmaceutically acceptable salt thereof;

[35] The compound of [33], wherein the optionally substituted alkyl group in B²is fluorine-substituted alkyl, or a pharmaceutically acceptable salt thereof;

[36] The compound of [33], wherein the optionally substituted heteroaryl group in B²is optionally substituted pyridyl, or a pharmaceutically acceptable salt thereof;

[37] The compound of [33], wherein the optionally substituted alkyl group in B²is optionally substituted benzyl, or a pharmaceutically acceptable salt thereof; [38] The compound of [21], wherein B¹is carbonyl, B²is optionally substituted alkylamino, optionally substituted dialkylamino, optionally substituted cycloalkylamino, optionally substituted heterocycloalkylamino, optionally substituted arylamino or optionally substituted heteroarylamino, or a pharmaceutically acceptable salt thereof;

[39] The compound of [38], wherein B²is optionally substituted arylamino or optionally substituted heteroarylamino, or a pharmaceutically acceptable salt thereof;

[40] The compound of [38], wherein B²is optionally substituted arylamino, or a pharmaceutically acceptable salt thereof;

[41] The compound of [38], wherein the optionally substituted heteroarylamino group in B²is optionally substituted pyridylamino, or a pharmaceutically acceptable salt thereof;

[42] The compound of [38], wherein the optionally substituted alkylamino group in B²is optionally substituted benzylamino, or a pharmaceutically acceptable salt thereof;

[43] The compound of any one of [21] to [42], wherein p is 0 and q is 1, or a pharmaceutically acceptable salt thereof;

[44] The compound of any one of [21] to [42] of formula (5):

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or a pharmaceutically acceptable salt thereof;

[45] The compound of any one of [21] to [42] of formula (6):

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or a pharmaceutically acceptable salt thereof;

[46] The compound of any one of [21] to [42], wherein p is 1 and q is 2, or a pharmaceutically acceptable salt thereof;

[47] The compound of any one of [21] to [42], wherein p is 2 and q is 2, or a pharmaceutically acceptable salt thereof;

[48] The compound of any one of [21] to [42], wherein p is 0 and q is 2, or a pharmaceutically acceptable salt thereof;

[49] The compound of any one of [21] to [42] of formula (7):

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or a pharmaceutically acceptable salt thereof;

[50] The compound of any one of [21] to [42] of formula (8):

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or a pharmaceutically acceptable salt thereof;

[51] The compound of any one of [21] to [50], wherein R_Bis methyl or ethyl, or a pharmaceutically acceptable salt thereof;

[52] The compound of any one of [8] to [51], wherein A is hydroxyl, or a pharmaceutically acceptable salt thereof;

[53] The compound of any one of [8] to [51], wherein A is carbamoyl, or a pharmaceutically acceptable salt thereof;

[54] The compound of any one of [4] to [53], wherein R_Dis chlorine atom, fluorine atom or methyl, or a pharmaceutically acceptable salt thereof;

[55] The compound of [54], wherein R_Cis alkyl, or a pharmaceutically acceptable salt thereof;

[56] The compound of [54], wherein R_Cis methyl or ethyl, or a pharmaceutically acceptable salt thereof;

[57] The compound of [56], wherein R_Eis hydrogen atom, or a pharmaceutically acceptable salt thereof;

[58] The compound of any one of [4] to [57], wherein A and nitrogen atom on which adamantyl group is substituted are arranged in E-configuration, or a pharmaceutically acceptable salt thereof;

[59] A medicament, comprising as the active ingredient the compound of any one of [1] to [58] or a pharmaceutically acceptable salt thereof;

[60] A therapeutic agent for type II diabetes, abnormal glucose tolerance, hyperglycemia, insulin resistance, dyslipidemia, hypertension, arteriosclerosis, angiostenosis, obesity, cognitive disorder, dementia, Alzheimer disease, syndrome X, depression, cardiovascular disease or atherosclerosis, comprising as the active ingredient the compound of any one of [1] to [58] or a pharmaceutically acceptable salt thereof;

[61] A therapeutic agent for diabetes, insulin resistance or type II diabetes, comprising as the active ingredient the compound of any one of [1] to [58] or a pharmaceutically acceptable salt thereof;

[62] A therapeutic agent for arteriosclerosis or atherosclerosis, comprising as the active ingredient the compound of any one of [1] to [58] or a pharmaceutically acceptable salt thereof;

[63] A therapeutic agent for syndrome X, comprising as the active ingredient the compound of any one of [1] to [58] or a pharmaceutically acceptable salt thereof;

[64] A therapeutic agent for obesity, comprising as the active ingredient the compound of any one of [1] to [58] or a pharmaceutically acceptable salt thereof;

[65] A therapeutic agent for cognitive disorder, dementia, Alzheimer disease or depression, comprising as the active ingredient the compound of any one of [1] to [58] or a pharmaceutically acceptable salt thereof;

[66] A therapeutic agent for dyslipidemia, comprising as the active ingredient the compound of any one of [1] to [58] or a pharmaceutically acceptable salt thereof; or

[67] A therapeutic agent for hypertension, comprising as the active ingredient the compound of any one of [1] to [58] or a pharmaceutically acceptable salt thereof.

The invention also relates to the following embodiments:

[68] A compound of formula (1):

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wherein R_Aand R_Bare each independently optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, or a group of formula: —Rw—Rx—Ry—Rz;

Rw is, independently when it exists more than one, optionally substituted alkylene;

Ry is, independently when it exists more than one, a single bond or optionally substituted alkylene;

R³and R⁴are each independently hydrogen atom or optionally substituted alkyl;

n is 0, 1 or 2;

R_Cis optionally substituted alkyl, optionally substituted cycloalkyl or optionally substituted cycloalkylalkyl;

R_Dis hydrogen atom, halogen atom, cyano, optionally substituted alkyl or optionally substituted cycloalkyl;

R_Eis hydrogen atom or optionally substituted alkyl;

R_Fis a group selected from the following formulae (G1):

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wherein one of hydrogen atoms is a bond, which may be optionally substituted;

provided that if both R_Aand R_Bare selected from the following group X, then R_Fis a group of the following formula (2):

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[69] The compound of [68] of formula (3):

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wherein R_Aand R_Bare each independently optionally substituted alkyl, optionally substituted cycloalkyl, or a group of formula: —Rw—Rx—Ry—Rz;

Rw is, independently when it exists more than one, optionally substituted alkylene;

Ry is, independently when it exists more than one, a single bond or optionally substituted alkylene;

R³and R⁴are each independently hydrogen atom or optionally substituted alkyl;

n is 0, 1 or 2;

R_Cis optionally substituted alkyl, optionally substituted cycloalkyl or optionally substituted cycloalkylalkyl;

R_Dis hydrogen atom, halogen atom, cyano, optionally substituted alkyl or optionally substituted cycloalkyl;

R_Eis hydrogen atom or optionally substituted alkyl;

the group X is optionally substituted alkyl, optionally substituted piperidinyl, optionally substituted pyrrolidinyl, optionally substituted arylalkyl, optionally substituted heteroarylalkyl, optionally substituted piperidinylalkyl, or optionally substituted pyrrolidinylalkyl, wherein the substituent is hydroxyl, oxo, halogen atom, cyano, nitro, alkyl, alkoxy, amino which may be optionally substituted by alkyl or arylalkyl, methylenedioxy, trihalomethyl, or trihalomethoxy; or a pharmaceutically acceptable salt thereof;

[70] The compound of [69], wherein R_Cis optionally substituted alkyl, R_Dis hydrogen atom, halogen atom or optionally substituted alkyl, R_Eis hydrogen atom, A is halogen atom, hydroxyl, cyano, or a group of formula: COOR¹, CONR¹R², SO₂NR¹R², COOR¹-substituted alkyl, CONR¹R²-substituted alkyl or SO₂NR¹R²-substituted alkyl, R¹and R²are each independently hydrogen atom or optionally substituted alkyl, or R¹and R²may combine each other together with the adjacent nitrogen atom to form an optionally substituted saturated heterocycle, or a pharmaceutically acceptable salt thereof;

[71] The compound of either [69] or [70], wherein R_Aand R_Bare each independently optionally substituted alkyl, optionally substituted cycloalkyl or optionally substituted cycloalkylalkyl, A is a group of formula: COOR¹, CONR¹R²or SO₂NR¹R², R¹and R²are each independently hydrogen atom or optionally substituted alkyl, or a pharmaceutically acceptable salt thereof;

[72] The compound of [71], wherein A is a group of formula: CONR¹R², R¹and R²are each independently hydrogen atom, or a pharmaceutically acceptable salt thereof;

[73] The compound of [72], wherein A and nitrogen atom on which adamantyl group is substituted are arranged in E-configuration, or a pharmaceutically acceptable salt thereof;

[74] The compound of either [69] or [70], wherein R_Ais optionally substituted cycloalkyl or optionally substituted cycloalkylalkyl, R_Bis optionally substituted alkyl, optionally substituted cycloalkyl, or a group of formula: —Rw—Rx—Ry—Rz wherein Rw, Rx, Ry and Rz are the same as defined in [69], or a pharmaceutically acceptable salt thereof;

[75] The compound of either [69] or [70], wherein R_Ais optionally substituted alkyl, R_Bis a group of formula: —Rw—Rx—Ry—Rz wherein Rw, Rx, Ry and Rz are the same as defined in [69], or a pharmaceutically acceptable salt thereof;

[76] The compound of [75], wherein Rx is a group of formula: —S(O)_n—, —C(O)—, —NR³—, —OC(O)—, —C(O)O—, —CONR³—, —NR³CO—, —SO₂NR³—, —NR³SO₂— or —NR³CONR⁴—, R³and R⁴are each independently hydrogen atom or optionally substituted alkyl, n is 0, 1 or 2, or a pharmaceutically acceptable salt thereof;

[77] The compound of [75], wherein Rx is oxygen atom, Rz is optionally substituted cycloalkyl, optionally substituted aryl, optionally substituted heteroaryl or optionally substituted heterocycloalkyl, or a pharmaceutically acceptable salt thereof;

[78] The compound of [75], wherein Rx is a bond, Rz is optionally substituted cycloalkyl or optionally substituted heterocycloalkyl, or a pharmaceutically acceptable salt thereof;

[79] The compound of [75], wherein Rx is a bond, Rz is substituted aryl, substituted heteroaryl or substituted heterocycloalkyl, in which the substituent is —COR⁵, —S(O)_nR⁵, —NR^7aCOR⁵, —SO₂NR^7aR^7b, —NR^7aCONR^7bR⁵, —OR⁶or —(CH₂)_mR⁶, R⁵is alkyl, cycloalkyl, aryl, heteroaryl or heterocycloalkyl, R⁶is cycloalkyl, aryl, heteroaryl or heterocycloalkyl, the alkyl, cycloalkyl, aryl, heteroaryl and heterocycloalkyl groups in R⁵and R⁶may be further optionally substituted by halogen atom, haloalkyl, haloalkoxy, alkyl, hydroxyl, alkoxy, —NR^8aR^8b, alkylsulfonyl, cyano, cycloalkyl, cycloalkylsulfonyl, alkoxyalkoxy, hydroxyalkoxy, cycloalkyloxyalkyl, cycloalkyloxy, haloalkoxyalkyl, hydroxyalkyl, alkoxyalkyl, NR^8aR^8b-substituted alkyl, alkylsulfonylalkyl, cyanoalkyl, cycloalkylalkyl, cycloalkylsulfonylalkyl, alkoxyalkoxyalkyl, hydroxyalkoxyalkyl or nitrogen-containing saturated heterocycle, R^7a, R^7b, R^8aand R^8bare each independently hydrogen atom or alkyl, n and m are each independently 0, 1 or 2, or a pharmaceutically acceptable salt thereof;

[80] The compound of any one of [74] to [79], wherein A is hydroxyl, or a pharmaceutically acceptable salt thereof;

[81] The compound of any one of [74] to [79], wherein A is carbamoyl, or a pharmaceutically acceptable salt thereof;

[82] The compound of either [80] or [81], wherein R_Dis chlorine atom, or a pharmaceutically acceptable salt thereof;

[83] The compound of [82], wherein R_Cis alkyl, or a pharmaceutically acceptable salt thereof;

[84] The compound of [82], wherein R_Cis methyl or ethyl, or a pharmaceutically acceptable salt thereof;

[85] The compound of any one of [80] to [84], wherein A and nitrogen atom on which adamantyl group is substituted are arranged in E-configuration, or a pharmaceutically acceptable salt thereof;

[86] A medicament, comprising as the active ingredient the compound of any one of [68] to [85] or a pharmaceutically acceptable salt thereof;

[87] A therapeutic agent for type II diabetes, abnormal glucose tolerance, hyperglycemia, insulin resistance, dyslipidemia, hypertension, arteriosclerosis, angiostenosis, obesity, cognitive disorder, dementia, Alzheimer disease, syndrome X, depression, cardiovascular disease or atherosclerosis, comprising as the active ingredient the compound of any one of [68] to [85] or a pharmaceutically acceptable salt thereof;

[88] A therapeutic agent for diabetes, insulin resistance or type II diabetes, comprising as the active ingredient the compound of any one of [68] to [85] or a pharmaceutically acceptable salt thereof;

[89] A therapeutic agent for arteriosclerosis or atherosclerosis, comprising as the active ingredient the compound of any one of [68] to [85] or a pharmaceutically acceptable salt thereof;

[90] A therapeutic agent for syndrome X, comprising as the active ingredient the compound of any one of [68] to [85] or a pharmaceutically acceptable salt thereof;

[91] A therapeutic agent for obesity, comprising as the active ingredient the compound of any one of [68] to [85] or a pharmaceutically acceptable salt thereof;

[92] A therapeutic agent for cognitive disorder, dementia, Alzheimer disease or depression, comprising as the active ingredient the compound of any one of [68] to [85] or a pharmaceutically acceptable salt thereof;

[93] A therapeutic agent for dyslipidemia, comprising as the active ingredient the compound of any one of [68] to [85] or a pharmaceutically acceptable salt thereof; or

[94] A therapeutic agent for hypertension, comprising as the active ingredient the compound of any one of [68] to [85] or a pharmaceutically acceptable salt thereof.

ADVANTAGEOUS EFFECT OF INVENTION

The compound of the invention is useful as a therapeutic and/or preventive agent for diseases including type II diabetes, abnormal glucose tolerance, hyperglycemia, insulin resistance, hypo-HDL-emia, hyper-LDL-emia, dyslipidemia, hyperlipidemia, hypertriglyceridemia, hypercholesterolemia, hypertension, arteriosclerosis, angiostenosis, atherosclerosis, obesity, cognitive disorder, glaucoma, retinopathy, dementia, Alzheimer disease, osteoporosis, immune disorder, syndrome X, depression, cardiovascular disease, neurodegenerative disease, etc.

BEST MODE FOR CARRYING OUT THE INVENTION

The invention is illustrated in more detail as below.

The number of substituents of “optionally substituted” or “substituted” groups herein is one or more without limitation if substitution is acceptable. Each definition of each group is applied to any groups which constitute a part of other groups or a substituent thereof, unless it is specified.

The term “halogen atom” includes fluorine atom, chlorine atom, bromine atom and iodine atom, preferably fluorine atom or chlorine atom.

The term “alkyl” includes C₁-C₅straight- and branched-chain alkyl, specifically methyl, ethyl, propyl, 1-methylethyl, butyl, 1-methylpropyl, 2-methylpropyl, 1,1-dimethylethyl, pentyl, 2,2-dimethylpropyl, etc.

The alkyl moiety of cycloalkylalkyl, arylalkyl, heteroarylalkyl, alkylsulfonyl, etc. includes the same as defined in the above alkyl.

The term “alkoxy” includes C₁-C₅alkoxy, specifically methoxy, ethoxy, propoxy, 1-methylethoxy, butoxy, 1-methylpropoxy, 2-methylpropoxy, 1,1-dimethylethoxy, pentyloxy, 2,2-dimethylpropoxy, etc.

The alkoxy moiety of alkoxyalkyl, etc. includes the same as defined in the above alkoxy.

The term “trihalomethyl” includes methyl substituted by three halogen atoms.

The term “trihalomethoxy” includes methoxy substituted by three halogen atoms.

The term “haloalkyl” includes alkyl substituted by halogen atom.

The term “haloalkoxy” includes alkoxy substituted by halogen atom. The term “alkylene” includes C₁-C₅straight- and branched-chain alkylene, specifically methylene, ethylene, trimethylene, tetramethylene, etc.

The term “cycloalkyl” includes C₃-C₈cycloalkyl, specifically cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl or cyclooctyl.

The cycloalkyl may have any double bonds in any substituent positions.

The cycloalkyl moiety of cycloalkyloxy, cycloalkylalkyl, etc. includes the same as defined in the above cycloalkyl.

The cycloalkyl includes any groups which are allowed to be fused with aryl or heteroaryl, for example any groups of the following formulae (B1):

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wherein any hydrogen atom of non-aromatic ring moiety is replaced with a bond.

The term “cycloalkylene” includes C₃-C₈cycloalkane, or any groups of the above formulae (B1) wherein two hydrogen atoms of non-aromatic ring moieties are replaced with bonds. The C₃-C₈cycloalkane specifically includes cyclopropane, cyclobutane, cyclopentane, cyclohexane, cycloheptane or cyclooctane.

The term “aryl” includes C₆-C₁₀aryl, specifically phenyl, 1-naphthyl, 2-naphthyl or indenyl. A preferable aryl includes phenyl.

The term “heteroaryl” includes 5 to 10-membered mono and multi-cyclic group containing one or more (e.g., 1 to 4) heteroatoms selected from nitrogen atom, sulfur atom or oxygen atom. Specifically, it includes furyl, thienyl, pyrrolyl, azepinyl, pyrazolyl, imidazolyl, oxazolyl, isoxazolyl, thiazolyl, isothiazolyl, 1,2,4-thiadiazolyl, 1,2,4-oxadiazolyl, 1,3,4-oxadiazolyl, 1,2,4-triazolyl, 1,2,3-triazolyl, pyranyl, pyridyl, pyridazinyl, pyrimidyl, pyrazinyl, indolyl, benzothienyl, benzofuryl, quinolyl, isoquinolyl, quinazolyl, quinoxalinyl, benzoxazolyl, benzothiazolyl, pyrazyl, triazinyl, tetrazolyl, imidazo[1,2-a]pyridyl, dibenzofuranyl, benzimidazolyl, cinnolyl, indazolyl, naphthyridyl, quinolonyl or isoquinolonyl. 5 to 6-membered cyclic group containing 1 to 3 heteroatoms selected from nitrogen atom, sulfur atom or oxygen atom is preferable, specifically pyridyl, pyrazinyl, thienyl, oxazolyl, 1,2,4-oxadiazolyl or pyridazinyl.

The aryl moiety of aryloxy, etc. includes the same as defined in the above aryl. The heteroaryl moiety of heteroaryloxy includes the same as defined in the above aryl.

The term “heterocycloalkyl” includes 5 to 6-membered ring heterocycloalkyl containing one or more (e.g., 1 to 3) heteroatoms selected from nitrogen atom, sulfur atom or oxygen atom, specifically pyrrolidinyl, imidazolidinyl, piperidinyl, morpholinyl, thiomorpholinyl, dioxothiomorpholinyl, hexamethyleneiminyl, oxazolidinyl, thiazolidinyl, imidazolidinyl, oxoimidazolidinyl, dioxoimidazolidinyl, oxooxazolidinyl, dioxooxazolidinyl, dioxothiazolidinyl, tetrahydropyridinyl, tetrahydrofuranyl or tetrahydropyranyl.

The term “heterocycloalkyl” also includes any groups wherein any hydrogen atom of thiomorpholin-1-oxide, morpholin-3-one, thiomorpholin-3-one, piperidin-4-one, piperidin-3-one, piperazine-2,6-dione, morpholin-2-one, piperazine, piperazin-2-one, piperazine-2,3-dione, piperazine-2,5-dione, tetrahydropyrimidin-2(1H)-one, 1,3-oxazinan-2-one, 1,3-oxazolidine, 1,3-thiazolidine, imidazolidin-2-one, 1,3-oxazolidin-2-one, 2,5-dihydro-1H-pyrrole, imidazolidine-2,4-dione, imidazolidin-4-one, 1,4-diazepane, 1,4-oxazepan, tetrahydro-2H-pyrane, tetrahydro-2H-thiopyrane, tetrahydro-2H-thiopyrane-1-oxide, tetrahydro-2H-thiopyrane-1,1-dioxide, 1,4-diazepan-3-one, 1,4-oxazepan-3-one, aziridine, azetidine, azetidine, pyrrolidine, azepane, azocane, pyrrolidin-2-one, piperidin-2-one, azepan-2-one, azocan-2-one, 1,5-dihydro-2H-pyrrol-2-one, 5,6-dihydropyridin-2(1H)-one, 1,5,6,7-tetrahydro-2H-azepin-2-one, 1,5,6,7-tetrahydro-2H-azepin-2-one, 5,6,7,8-tetrahydroazocin-2(1H)-one, 1,2,3,4-tetrahydropyridine, 1,2,3,6-tetrahydropyridine, 2,3,4,7-tetrahydro-1H-azepine, 1,2,3,4,5,8-hexahydroazocine, tetrahydrofuran, tetrahydrothiophene, 1,2-oxathiolane, etc. are replaced with bonds.

A preferable heterocycloalkyl includes pyrrolidyl, piperidyl, morpholinyl, thiomorpholinyl, dioxothiomorpholinyl, oxazolidinyl, more preferably pyrrolidyl or piperidyl.

The term “heterocycloalkyl” also includes any groups fused with aryl or heteroaryl, for example any groups wherein any hydrogen atoms of non-aromatic cyclic moieties of the following formulae (B2) or (B3) are replaced with bonds.

The term “nitrogen-containing saturated heterocycle” includes 5 to 6-membered nitrogen-containing saturated heterocycle, etc. which contain 1 to 2 nitrogen atoms and may contain oxygen atoms or sulfur atoms, specifically pyrrolidinyl, imidazolidinyl, piperidinyl, morpholinyl, thiomorpholinyl, dioxothiomorpholinyl, hexamethyleneiminyl, oxazolidinyl, thiazolidinyl, imidazolidinyl, oxoimidazolidinyl, dioxoimidazolidinyl, oxooxazolidinyl, dioxooxazolidinyl, dioxothiazolidinyl or tetrahydropyridinyl. A preferable one includes pyrrolidinyl, piperidinyl, thiomorpholinyl, dioxothiomorpholinyl, morpholinyl.

The term “aralkyl” includes C₇-C₁₂aralkyl wherein alkyl is substituted by aryl, specifically benzyl, 2-phenylethyl or 1-naphthylmethyl.

The aralkyl moiety of aralkyloxy includes the same as defined in the above aralkyl.

The substituents of “substituted alkyl”, “substituted alkoxy” and “substituted cycloalkyl” include halogen atom, hydroxyl, nitro, cyano, —OR¹⁰, —OCOR¹⁰, —COR¹⁰, —COOR¹⁰, C₃-C₆cycloalkyl, amino, carboxy, carbamoyl, —NHR¹⁰, —NR¹⁰R¹¹, —NR¹²COR¹⁰, —CONR¹⁰R¹¹, —NR¹²CONR¹⁰R¹¹, —NR¹²SO₂R¹⁰or —SO₂R¹⁰(wherein R¹⁰and R¹¹are each independently cycloalkyl, C₁-C₄alkyl, C₆-C₁₀aryl, heteroaryl or C₇-C₁₂aralkyl, which may further substituted by hydroxyl, halogen atom, C₁-C₄alkoxy, cycloalkoxy, C₁-C₄alkyl, cycloalkyl, haloalkyl, haloalkoxy, amino, C₁-C₄alkylamino or C₁-C₄dialkylamino, or R¹⁰and R¹¹may combine each other together with the adjacent nitrogen atom to form an optionally substituted saturated heterocycle; R¹²is hydrogen atom or alkyl). A preferable one includes halogen atom, hydroxyl, alkyl, haloalkoxy, alkylsulfonyl and alkoxy. More preferable one includes halogen atom and alkoxy.

The substituent of “substituted cycloalkyl” also includes alkyl which may be optionally substituted by aryl, alkoxy or halogen atom.

The substituent of the substituted cycloalkyl also includes optionally substituted aryl and optionally substituted heteroaryl.

The substituents of “substituted aryl” and “substituted heteroaryl” include halogen atom, hydroxyl, nitro, cyano, nitrogen-containing saturated heterocycle, cycloalkyl, cycloalkyloxy, C₁-C₄alkyl (wherein alkyl may be substituted by halogen atom, hydroxyl, amino, cycloalkyloxy, haloalkoxy, alkoxyalkoxy, cycloalkyl, alkoxy, alkylsulfonyl, cycloalkylsulfonyl, hydroxyalkoxy, etc.), C₁-C₄alkoxy (wherein alkoxy may be substituted by halogen atom, hydroxyl, alkoxy, etc.), —COR¹⁰, —OCOR¹⁰, —COOR¹⁰, carboxy, amino, —NHR¹⁰, —NR¹⁰R¹¹, —NHCOR¹⁰, —CONH₂, —CONHR¹⁰, —CONR¹⁰R¹¹, —SO₂NH₂, —SO₂NHR¹⁰, —SO₂NR¹⁰R¹¹, C₆-C₁₀aryl, C₆-C₁₀aryloxy, C₇-C₁₂aralkyloxy (wherein aryl, aryloxy or aralkyloxy may be substituted by hydroxyl, halogen atom, C₁-C₄alkoxy, etc.), —SO₂R¹⁰, cycloalkylsulfonyl (wherein R¹⁰and R¹¹are the same as defined above), etc.

A preferable substituent includes nitrogen-containing saturated heterocycle, alkylsulfonyl, halogen atom, hydroxyl, alkyl (which may be optionally substituted by alkoxy or halogen atom), or alkoxy (which may be optionally substituted by alkoxy or halogen atom), etc. More preferable one includes halogen atom, alkylsulfonyl, alkyl (which may be optionally substituted by alkoxy or halogen atom), or alkoxy (which may be optionally substituted by halogen atom).

The substituent of the substituted aryl also includes C₁-C₃alkylenedioxy such as methylenedioxy or ethylenedioxy.

The term “substituted aryl” includes any groups fused with cycloalkyl and cycloheteroalkyl, for example any groups of the above formulae (B1) and the following formulae (B2):

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wherein any hydrogen atoms of aromatic ring moieties are replaced with bonds, which may be further optionally substituted by the above listed substituents.

The term “substituted heteroaryl” includes any groups fused with cycloalkyl and cycloheteroalkyl, for example any groups of the following formula (B3):

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wherein any hydrogen atoms of aromatic ring moiety are replaced with bonds, which may be further optionally substituted by the above listed substituents.

The substituents of aryl and heteroaryl moieties of “substituted aralkyl” and “substituted heteroarylalkyl” include any groups listed as the substituents of “substituted aryl” and “substituted heteroaryl”.

The substituent of alkyl moiety of “substituted aralkyl” includes any groups listed as the substituents of “substituted alkyl”.

The substituent of “substituted heterocycloalkyl” or “substituted nitrogen-containing saturated heterocycle” includes C₁-C₄alkyl (which may be optionally substituted by aryl, alkoxy or halogen atom), optionally substituted aryl, optionally substituted heteroaryl, —OR¹⁰, —OCOR¹⁰, —COR¹⁰, —COOR¹, C₃-C₆cycloalkyl, amino, carboxy, carbamoyl, —NHR¹⁰, —NR¹⁰R¹¹, NR¹²COR¹⁰, —CONR¹⁰R¹¹, —NR¹²COR¹⁰R¹¹, —NR¹²SO₂R¹⁰or —SO₂R¹⁰(wherein R¹⁰and R¹¹are each independently cycloalkyl, C₁-C₄alkyl, C₆-C₁₀aryl, heteroaryl or C₇-C₁₂aralkyl, which may be further optionally substituted by hydroxyl, halogen atom, C₁-C₄alkoxy, cycloalkoxy, C₁-C₄alkyl, cycloalkyl, haloalkyl, haloalkoxy, aryl, heteroaryl, amino, C₁-C₄alkylamino or C₁-C₄dialkylamino, or R¹⁰and R¹¹may combine each other together with the adjacent nitrogen atom to form an optionally substituted saturated heterocycle; R¹²is hydrogen atom or alkyl). A preferable substituent includes alkyl, C₆-C₁₀aryl, heteroaryl, —COR¹⁰, —CON¹⁰R¹¹or —SO₂R¹⁰.

A preferable substituent of alkyl of R⁵or R⁶includes halogen atom, hydroxyl or alkoxy.

A preferable substituent of cycloalkyl, aryl, heteroaryl and heterocycloalkyl of R⁵or R⁶includes halogen atom, hydroxyl, alkyl (which may be optionally substituted by hydroxyl, alkoxy or halogen atom), and alkoxy (which may be optionally substituted by hydroxyl, alkoxy or halogen atom).

A preferable substituent of R¹or R²includes halogen atom, hydroxyl, alkoxy, arylsulfonyl or pyridyl.

Alkylamino means amino group substituted by alkyl group.

Dialkylamino means amino group substituted by the same or different two alkyl groups.

Cycloalkylamino means amino group substituted by cycloalkyl group as well as cyclic amino group including pyrrolidino or piperidino.

Heterocycloalkylamino means amino group substituted by heterocycloalkyl group and also includes cyclic amino group including morpholino or thiomorpholino.

Arylamino is amino substituted by aryl group.

Heteroarylamino is amino substituted by heteroaryl group.

The substituent of “substituted alkylamino”, “substituted dialkylamino”, “substituted cycloalkylamino”, “substituted heterocycloamino”, “substituted arylamino” or “substituted heteroarylamino” includes any groups listed as the substituents of “substituted alkyl”, “substituted dialkyl”, “substituted cycloalkyl”, “substituted heterocycloalkyl”, “substituted aryl” or “substituted heteroaryl”.

A group selected from (G2) preferably includes adamantyl.

Adamantyl may be optionally substituted, and a preferable substituent position includes a position where A is bonded in the following formula:

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A group, wherein the substituent A and nitrogen atom, on which the adamantyl group is substituted, are arranged in E-configuration is more preferable.

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E-Configuration

The “pharmaceutically acceptable salt” includes alkali metal salt such as potassium salt or sodium salt, alkaline earth metal salt such as calcium salt or magnesium salt, ammonium salt, a water-soluble amine addition salt such as ammonium salt or N-methylglucamine (meglumine), or a lower alkanolammonium salt of an organic amine; and, for example, hydrochloride, hydrobromide, hydroiodide, nitrate, sulfate, hydrogen sulfate, phosphate, acetate, lactate, citrate, tartrate, hydrogen tartrate, succinate, maleate, fumarate, gluconate, saccharate, benzoate, methanesulfonate, ethanesulfonate, benzenesulfonate, para-toluenesulfonate, or pamoate [1,1′-methylene-bis-(2-hydroxy-3-naphthoate)], etc.

A resultant salt form of the inventive compound may be directly purified to give a salt of the inventive compound, or a free form of the inventive compound may be dissolved or suspended in an appropriate organic solvent to form a salt thereof by the addition of an acid or a base in a conventional manner

The inventive compound and a pharmaceutically acceptable salt thereof may exist in the form of adducts with water or various solvents which are included in the invention. The invention includes all tautomers, all possible stereoisomers and all crystalline forms of the inventive compound.

The inventive compound or a pharmaceutically acceptable salt thereof may be orally or parenterally administered (e.g., intravenous, subcutaneous, or drops, intramuscular injection, subcutaneous injection, internal nasal formulation, eye-drop, suppository, transdermal administration formulation including ointment, cream or lotion, etc.) for medical use. A dosage form for oral administration includes tablet, capsule, pill, granule, powder, solution, syrup and suspension, etc. and a dosage form for parenteral administration includes aqueous or oil preparation for injection, ointment, cream, lotion, aerosol, suppository, patch, etc.

The preparation may be formulated by using conventional known techniques and comprise a conventionally acceptable carrier, excipient, binder, stabilizer, lubricant, disintegrant, etc. The preparation for injection may further comprise an acceptable buffer, solubilizing agent, isotonic agent, etc. The preparation may also optionally comprise flavoring agent.

The excipient may include an organic excipient including sugar derivative such as lactose, sucrose, glucose, mannitol, sorbitol; starch derivative such as corn starch, potato starch, alpha-starch, dextrin, carboxymethyl starch; cellulose derivative such as crystalline cellulose, low-substituted hydroxypropyl cellulose, hydroxypropyl methylcellulose, carboxymethylcellulose, carboxymethylcellulose calcium, internally-crosslinked carboxymethylcellulose sodium; gum arabic; dextran; pullulan; and an inorganic excipient including silicate derivative such as light anhydrous silicic acid, synthetic aluminum silicate, magnesium aluminometasilicate; phosphate such as calcium phosphate; carbonate such as calcium carbonate; sulfate such as calcium sulfate.

The lubricant may include stearic acid, metal stearate such as calcium stearate, magnesium stearate; talc; colloid silica; wax such as VEEGUM®, spermaceti; boric acid; adipic acid; sulfate such as sodium sulfate; glycol; fumaric acid; sodium benzoate; DL-leucine; fatty acid sodium salt; lauryl sulfate such as sodium lauryl sulfate, magnesium lauryl sulfate; silicic acid such as anhydrous silicic acid, silicic acid hydrate; and the above starch derivative, etc.

The binder may include polyvinylpyrrolidone, macrogol, and the above substances listed as the excipient.

The disintegrant may include the above substances listed as the excipient and chemically modified starch-cellulose such as croscarmellose sodium, sodium carboxymethyl starch or cross-linked polyvinylpyrrolidone.

The stabilizer may include paraoxybenzoic acid ester such as methylparaben, propylparaben; alcohol such as chlorobutanol, benzyl alcohol, phenylethyl alcohol; benzalkonium chloride; phenols such as phenol, cresol; thimerosal; dehydroacetic acid; and sorbic acid.

The flavoring agent may include conventionally-used sweetener, acidulant, perfume, etc.

A tablet for oral administration may comprise an excipient together with various disintegrants as well as granulating binders. A lubricant is often very useful for tablet formulation. The similar type of the solid composition may be used as a bulking agent of a gelatin capsule which may be combined by any ingredients, preferably lactose or milk sugar, or high-molecular-weight polyethyleneglycol.

The active ingredient of aqueous suspension and/or elixir for oral administration may be combined with a diluent together with various sweetening agents, flavoring agents, coloring agents or dyes, or if desired, emulsifiers and/or suspending agents. The diluent includes water, ethanol, propylene glycol, glycerin and a mixture thereof. It is conveniently included in feed or drinking water for animal in a concentration of 5-5000 ppm, preferably 25-5000 ppm.

A solution of the active ingredient for sterile injection may be usually prepared for parenteral administration (intramuscular, intraperitoneal, subcutaneous and intravenous use). A solution of the inventive compound in sesame oil or peanut oil or aqueous propylene glycol may be used. The aqueous solution should be appropriately adjusted and buffered preferably in more than 8 of pH, if needed, to firstly prepare an isotonic solution of a liquid diluent. The aqueous solution is suitable for intravenous injection. The oil solution is suitable for intra-articular, intramuscular and subcutaneous injections. All solutions may be easily prepared under sterile conditions by using typical formulation techniques known to those skilled in the art.

The inventive compound or a pharmaceutically acceptable salt thereof for the intranasal or inhalation administration may be provided in the solution or suspension form squeezed out or released by a patient from a pump spray vessel, or as an aerosol spray from a pressurized vessel or a nebulizer with using an appropriate propellant including dichlorodifluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane or carbon dioxide or using other appropriate gas. A dosage unit in the pressurized aerosol may be determined by a bulb which provides a certain measured amount of the active ingredient. A solution or suspension of the active compound may be contained in the pressurized vessel or nebulizer.

A capsule and cartridge for an inhaler or insufflator (e.g., prepared from gelatin) may be formulated to contain the inventive compound and a powder composition of appropriate powder bases including lactose or starch.

The inventive compound or a pharmaceutically acceptable salt thereof may be also formulated in a composition for the anus such as a suppository or retension enema comprising conventional suppository bases including cacao butter or other glycerides.

A usage of the inventive compound or a pharmaceutically acceptable salt thereof depends on conditions, ages, administration methods, etc., and for example, it is 0.01 mg, preferably 1 mg, as a lower limit and 5000 mg, preferably 500 mg, as a upper limit per day at one time or in several divided doses for adults for oral administration, preferably depending on conditions. It is expected to be effective in 0.01 mg, preferably 0.1 mg, as a lower limit and 1000 mg, preferably 30 mg, as an upper limit per day at one time or in several divided doses for adults for intravenous administration depending on conditions.

The inventive compound may be used in combination with a drug, referred to as a combination drug hereinafter, including a therapeutic agent for diabetes or diabetic complication, anti-hyperlipidemia, antihypertensive, anti-obesity agent, diuretic, etc. for the purpose of enhancement of efficacy. The inventive compound may be administered to a subject simultaneously with a combination drug or at intervals without limitation. The inventive compound may be formulated with a combination drug to prepare a drug combination. A dosage of a combination drug may be optionally selected on the basis of clinically acceptable doses. A compounding ratio of the inventive compound and a combination drug may be optionally selected depending on administration subjects, administration routes, intended diseases, conditions and a combination thereof. For example, 0.01-100 parts of a combination drug to 1 part of the inventive compound by weight may be administered for human.

The therapeutic agent for diabetes includes insulin formulations (e.g., animal insulin formulations extracted from bovine or swine pancreas; human insulin formulations genetically engineered by using E. coli or yeast cells, etc.), insulin resistance improving agents (e.g., pioglitazone or a hydrochloride salt thereof, troglitazone, rosiglitazone or a maleate salt thereof, G1-262570, JTT-501, MCC-555, YM-440, KRP-297, CS-011, etc.), alpha-glucosidase inhibitors (e.g., voglibose, acarbose, miglitol, emiglitate, etc.), biguanides (e.g., metformin, etc.), insulin secretion stimulators (e.g., sulfonylurea agents such as tolbutamide, glibenclamide, gliclazide, chlorpropamide, tolazamide, acetohexamide, glyclopyramide, glimepiride; repaglinide, senaglinide, nateglinide, mitiglinide, etc.), dipeptidyl peptidase-IV (DPP-IV) inhibitors (e.g., sitagliptin or a phosphate salt thereof, vildagliptin, alogliptin or a benzoate salt thereof, denagliptin or a tosylate salt thereof, etc.), GLP-1, GLP-1 analogs (exenatide, liraglutide, SUN-E7001, AVE010, BIM-51077, CJC1131, etc.), protein tyrosine phosphatase inhibitors (e.g., vanadic acid, etc.), β3 agonists (e.g., GW-427353B, N-5984, etc.).

The therapeutic agent for diabetic complication includes aldose reductase inhibitors (e.g., tolrestat, epalrestat, zenarestat, zopolrestat, minalrestat, fidarestat, ranirestat, SK-860, CT-112, etc.), neurotrophic factors (e.g., NGF, NT-3, BDNF, etc.), PKC inhibitors (e.g., LY-333531, etc.), AGE inhibitors (e.g., ALT946, pimagedine, piratoxatin, N-phenacylthiazolium bromide (ALT766), etc.), active oxygen removers (e.g., thioctic acid, etc.), cerebral blood-vessel dilators (e.g., tiapride, mexiletine, etc.). The anti-hyperlipidemia includes HMG-CoA reductase inhibitors (e.g., pravastatin, simvastatin, lovastatin, atorvastatin, fluvastatin, pitavastatin or a sodium salt thereof, etc.), squalene synthetase inhibitors, ACAT inhibitors, etc. The antihypertensive includes angiotensin-converting enzyme inhibitors (e.g., captopril, enalapril, alacepril, delapril, lisinopril, imidapril, benazepril, cilazapril, temocapril, trandolapril, etc.), angiotensin II antagonists (e.g., olmesartan, medoxomil, candesartan, cilexetil, losartan, eprosartan, valsartan, telmisartan, irbesartan, tasosartan, etc.), calcium antagonists (e.g., nicardipine hydrochloride, manidipine hydrochloride, nisoldipine, nitrendipine, nilvadipine, amlodipine, etc.), etc.

The anti-obesity agent includes central anti-obesity agents (e.g., phentermine, sibutramine, amfepramone, dexamphetamine, mazindol, SR-141716A, etc.), pancreatic lipase inhibitors (e.g., orlistat, etc.), peptidic anorexiants (e.g., leptin, CNTF (ciliary neurotrophic factor), etc.), cholecystokinin agonists (e.g., lintitript, FPL-15849, etc.), etc. The diuretic includes xanthin derivative (e.g., sodium salicylate and theobromine, calcium salicylate and theobromine, etc.), thiazide preparations (e.g., ethiazide, cyclopenthiazide, trichlormethiazide, hydrochlorothiazide, hydroflumethiazide, bentyl hydrochlorothiazide, penflutizide, polythiazide, methyclothiazide, etc.), anti-aldosterone preparations (e.g., spironolactone, triamterene, etc.), carbonic anhydrase inhibitors (e.g., acetazolamide, etc.), chlorobenzenesulfonamide preparations (e.g., chlortalidone, mefruside, indapamide, etc.), azosemide, isosorbide, ethacrynic acid, piretanide, bumetanide, flosemide, etc.

The combination drug preferably includes GLP-1, GLP-1 analogs, alpha-glucosidase inhibitors, biguanides, insulin secretagogues, insulin resistance improving agents, DPP-IV inhibitors. The two or more combination drugs may be combined in any proportions.

The inventive compound may be combined with a combination drug to reduce dosages thereof within safe limits in terms of side effects of drugs. For example, biguanides may be reduced in lower doses than usual ones. Thus, side effects caused by the drugs may be safely prevented. In addition, dosages of a therapeutic agent for diabetic complication, anti-hyperlipidemia, antihypertensive, etc. may be reduced, and hence, side effects caused by the drugs may be effectively prevented.

Specific examples of the inventive compound of the general formula (1) may include the following compounds.

[Chemical Formula 19]

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A
R_C
R_D
—NR_AR_B

CONH₂
Et
H

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CONH₂
Et
Cl

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CONH₂
Me
Me

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CONH₂
Me
Cl

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OH
Me
Cl

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CONH₂
Me
Cl

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[Chemical Formula 20]

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A
R_C
R_D
—NR_AR_B

OH
Me
Cl

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CONH₂
Me
Cl

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OH
Me
Cl

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CONH₂
Me
Cl

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OH
Me
Cl

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CONH₂
Me
Cl

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OH
Me
Cl

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[Chemical Formula 21]

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A
R_C
R_D
—NR_AR_B

OH
Me
Cl

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CONH₂
Me
H

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CONH₂
Me
Cl

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CONH₂
Me
H

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OH
Me
H

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CONH₂
Me
H

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OH
Me
Cl

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A preparation method of the inventive compound of formula (1) is illustrated by an example as follows, but the invention is not limited thereto.

A compound of formula (1) may be synthesized by the following methods.

Preparation 1

Among a compound of formula (1), a compound of formula (A-8) or a salt thereof may be prepared by the following methods.

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(In the above scheme, R_A, R_B, R_C, R_D, R_Eand R_Fare the same as defined above. R is methyl, ethyl or benzyl, etc. X is halogen atom, etc. Provided that R_Dis not halogen atom.)

Step 1:

R_AR_BNH (A-1) gives thiosemicarbazide (A-2) in the step.

Amine (A-1) may be reacted with 1,1′-thiocarbonyldiimidazole or thiophosgene in an inert solvent usually at −10° C. to 50° C. for 0.5 to 48 hours, and then, further reacted with hydrazine or hydrazine monohydrate usually at −10° C. to reflux temperature for 0.5 to 8 hours to give thiosemicarbazide (A-2). The inert solvent includes ether type solvents such as tetrahydrofuran, diethylether, dioxane or 1,2-dimethoxyethane, hydrocarbon solvents such as toluene or benzene, polar organic solvents such as N,N-dimethylformamide, N,N-dimethylacetamide, N-methyl-2-pyrrolidinone, 1,3-dimethyl-2-imidazolidinone or dimethylsulfoxide, halogenated hydrocarbon solvents such as dichloromethane, chloroform or 1,2-dichloroethane, or a mixed solvent thereof.

Alternatively, amine (A-1) is reacted with aryl halothioformate in an inert solvent usually at −40° C. to 50° C. for 0.5 to 24 hours in the presence of a base. The obtained thiocarbamate may be reacted with hydrazine or hydrazine monohydrate in an inert solvent usually at −10° C. to reflux temperature for 0.5 to 24 hours to give thiosemicarbazide (A-2). The inert solvent includes ether type solvents such as tetrahydrofuran, diethylether, dioxane or 1,2-dimethoxyethane, hydrocarbon solvents such as toluene or benzene, halogenated hydrocarbon solvents such as dichloromethane, chloroform or 1,2-dichloroethane, polar organic solvents such as N,N-dimethylformamide, N,N-dimethylacetamide, N-methyl-2-pyrrolidinone, 1,3-dimethyl-2-imidazolidinone or dimethylsulfoxide, water, or a mixed solvent thereof. The base may be optionally selected from nitrogen-containing organic bases such as triethylamine, diisopropylethylamine, tributylamine, 1,5-diazabicyclo [4.3.0] non-5-ene (DBN), 1,4-diazabicyclo [2.2.2] octane (DABCO), 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU), pyridine, dimethylaminopyridine, picoline or N-methylmorpholine (NMM), etc., or inorganic bases such as sodium bicarbonate, potassium bicarbonate, sodium carbonate, potassium carbonate, sodium hydroxide or potassium hydroxide, etc.

Step 2:

Thiosemicarbazide (A-2) may be reacted with alpha-halo ketoester (A-3) in an inert solvent usually at −10° C. to reflux temperature for 0.5 to 48 hours to give Compound (A-4). In the reaction, nitrogen-containing organic bases such as triethylamine, diisopropylethylamine, tributylamine, 1,5-diazabicyclo[4.3.0]non-5-ene (DBN), 1,4-diazabicyclo[2.2.2]octane (DABCO), 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU), pyridine, dimethylaminopyridine, picoline or N-methylmorpholine (NMM), or inorganic bases such as sodium bicarbonate, potassium bicarbonate, sodium carbonate, potassium carbonate, sodium hydroxide or potassium hydroxide may be optionally added to the reaction mixture. The inert solvent includes ether type solvents such as tetrahydrofuran, diethylether, dioxane or 1,2-dimethoxyethane, hydrocarbon solvents such as toluene or benzene, polar organic solvents such as dimethylsulfoxide, alcoholic solvents such as methanol, ethanol or 2-propanol, halogenated hydrocarbon solvents such as dichloromethane, chloroform or 1,2-dichloroethane, water, or a mixed solvent thereof, etc.

Step 3:

Compound (A-4) may be treated with an organic acid such as propionic acid, acetic acid, formic acid, methanesulfonic acid, toluenesulfonic acid or trifluoroacetic acid, or a mineral acid such as hydrogen chloride, sulfuric acid or hydrogen bromide, etc. in an inert solvent or in neat usually at −10° C. to reflux temperature for 0.5 to 48 hours to give pyrazole (A-5). The inert solvent includes ether type solvents such as tetrahydrofuran, diethylether, dioxane or 1,2-dimethoxyethane, hydrocarbon solvents such as toluene or benzene, polar organic solvents such as dimethylsulfoxide, alcoholic solvents such as methanol, ethanol or 2-propanol, water, or a mixed solvent thereof, and any stable solvents under the reaction condition may be used among them.

Step 4:

Compound (A-2) gives pyrazole (A-5) in the step without isolating or purifying Compound (A-4).

The reaction system of Step 2 or a concentration residue thereof may be treated with the acid listed in Step 3 at −10° C. to reflux temperature for 0.5-48 hours to give pyrazole (A-5). The reaction may be also carried out with removing a solvent from the reaction system to give pyrazole (A-5) in the step. The solvent in an addition of acid may be selected from ether type solvents such as tetrahydrofuran, diethylether, dioxane or 1,2-dimethoxyethane, hydrocarbon solvents such as toluene or benzene, polar organic solvents such as dimethylsulfoxide, alcoholic solvents such as methanol, ethanol or 2-propanol, water, or a mixed solvent thereof, which may be stable under the reaction condition.

Step 5:

Compound (A-5) is treated with a base, followed by treating with an alkylating agent such as dialkyl sulfate or alkyl halide at −78° C. to reflux temperature to give a compound of formula (A-6) in the step.

The base includes inorganic bases such as potassium carbonate, sodium carbonate, potassium bicarbonate, sodium bicarbonate, lithium carbonate, sodium hydroxide or potassium hydroxide, metal hydrides such as sodium hydride, lithium hydride or potassium hydride, metal alkoxides such as sodium methoxide, potassium methoxide, sodium ethoxide, potassium ethoxide, sodium tertiary-butoxide or potassium tertiary-butoxide, potassium hexamethyldisilazide, sodium hexamethyldisilazide, lithium hexamethyldisilazide, or lithium diisopropylamide. The solvent includes ether type solvents such as diethylether, diisopropylether, tetrahydrofuran or 1,4-dioxane, N,N-dimethylformamide, N,N-dimethylacetamide, N-methyl-2-pyrrolidinone, 1,3-dimethyl-2-imidazolidinone, or dimethylsulfoxide.

Step 6:

An ester group of Compound (A-6) is deprotected to give a carboxylic acid compound (A-7) in the step. The step may be carried out according to methods described in Greene's Protective Groups in Organic Synthesis, John Wiley & Sons Inc., 1981.

Specifically, the following methods are carried out in the step.

(A) Compound (A-6) wherein R is methyl, ethyl, etc. may be converted to a corresponding carboxylic acid by alkali hydrolysis or acid hydrolysis. Specifically, Compound (A-6) may be treated in the presence of a hydroxide of alkali metal or alkaline-earth metal such as sodium hydroxide, potassium hydroxide, lithium hydroxide or magnesium hydroxide in water, or water and alcoholic solvents such as methanol, ethanol, 2-propanol or butanol, ether type solvents such as diethylether, diisopropylether, tetrahydrofuran or 1,4-dioxane, aromatic hydrocarbon solvents such as benzene, toluene or xylene, or a mixed solvent thereof usually at room temperature to reflux temperature for 0.5 to 48 hours to give Compound (A-7).

(B) Compound (A-6) wherein R is benzyl may be reacted in the presence of a metal catalyst such as palladium/carbon, palladium hydroxide, platinum, platinum oxide or nickel, etc. with the addition of hydrogen chloride, ammonium formate, if needed, under hydrogen gas to give Compound (A-7). The solvent includes alcoholic solvents such as methanol, ethanol, 2-propanol or butanol, ether type solvents such as diethylether, diisopropylether, tetrahydrofuran or 1,4-dioxane, aromatic hydrocarbon solvents such as benzene, toluene or xylene, ester type solvents such as ethyl acetate or methyl acetate, organic acids such as acetic acid, or a mixed solvent thereof

Step 7:

Carboxyl group of Compound (A-7) is activated, followed by reacting with amine R_ER_FNH or a salt thereof to give Compound (A-8) in the step.

The activation method of carboxy group includes a method wherein carboxy group is converted to acid anhydride, mixed acid anhydride, acid halide, activated ester or acid azide, or a method wherein a condensing agent is used.

Using the acid halide method, Compound (A-7) may be reacted with a halogenating agent such as oxalyl chloride, thionyl chloride, phosphorus oxychloride or phosphorus pentachloride to give an acid halide, followed by reacting with amine R_ER_FNH or a salt thereof in the presence of a base to give Compound (A-8). The base includes organic bases such as triethylamine, diisopropylethylamine, tributylamine, 1,5-diazabicyclo[4.3.0]non-5-ene (DBN), 1,4-diazabicyclo[2.2.2]octane (DABCO), 1,8-diazabicyclo [5.4.0] undec-7-ene (DBU), pyridine, dimethylaminopyridine, picoline or N-methylmorpholine (NMM), or inorganic bases such as sodium bicarbonate, potassium bicarbonate, sodium carbonate, potassium carbonate, sodium hydroxide or potassium hydroxide, without any limitation. Any solvents which may be stable under the reaction condition may be used in the step. For example, such solvents include halogenated hydrocarbon solvents such as dichloromethane, chloroform, 1,2-dichloroethane or carbon tetrachloride, ether type solvents such as diethylether, diisopropylether, tetrahydrofuran or 1,4-dioxane, aromatic hydrocarbon solvents such as benzene, toluene or xylene, ester type solvents such as ethyl acetate or methyl acetate, water, or a mixture thereof. The reaction temperature is in the range of −80° C. to reflux temperature, usually at −20° C. to ice-cooling temperature.

The reaction time is in the range of 10 minutes to 48 hours.

Using the mixed acid anhydride method, Compound (A-7) may be reacted with an acid halide in the presence of a base to give a mixed acid anhydride, followed by reacting with amine R_ER_FNH or a salt thereof to give Compound (A-8). The acid halide includes methoxycarbonyl chloride, ethoxycarbonyl chloride, isopropyloxycarbonyl chloride, isobutyloxycarbonyl chloride, para-nitrophenoxy carbonyl chloride or t-butylcarbonyl chloride. The base includes organic bases such as triethylamine, diisopropylethylamine, tributylamine, 1,5-diazabicyclo[4.3.0]non-5-ene (DBN), 1,4-diazabicyclo[2.2.2]octane (DABCO), 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU), pyridine, dimethylaminopyridine, picoline or N-methylmorpholine (NMM), or inorganic bases such as sodium bicarbonate, potassium bicarbonate, sodium carbonate or potassium carbonate, without any limitation. Any solvents which may be stable under the reaction condition may be used in the step. For example, such solvents include halogenated hydrocarbon solvents such as dichloromethane, chloroform, 1,2-dichloroethane or carbon tetrachloride, ether type solvents such as diethylether, diisopropylether, tetrahydrofuran or 1,4-dioxane, aromatic hydrocarbon solvents such as benzene, toluene or xylene, ester type solvents such as ethyl acetate or methyl acetate, water, or a mixture thereof. The reaction temperature is in the range of −80° C. to reflux temperature, usually at −20° C. to ice-cooling temperature. The reaction time is in the range of 30 minutes to 48 hours.

Compound (A-7) may be reacted with amine R_ER_FNH or a salt thereof using a condensing agent in the presence or absence of a base to give Compound (A-8). The condensing agent includes substances listed in The Experimental Chemistry (Jikken Kagaku Koza), edited by The Chemical Society of Japan, Maruzen, Vol. 22, e.g., phosphoric acid esters such as diethyl cyanophosphate or diphenyl phosphoryl azide, carbodiimides such as 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride or dicyclohexylcarbodiimide, combinations of disulfides such as 2,2′-dipyridyl disulfide with phosphines such as triphenylphosphine, phosphorus halides such as N,N′-bis(2-oxo-3-oxazolidinyl)phosphinic chloride, combinations of azodicarboxylic acid diesters such as diethyl azodicarboxylate with phosphines such as triphenylphosphine, 2-halo-1-lower alkylpyridinium halides such as 2-chloro-1-methylpyridinium iodide, 1,1′-carbonyldiimidazole, diphenyl phosphoryl azide (DPPA), diethylphosphoryl cyanide (DEPC), dicyclohexylcarbodiimide (DCC), carbonyldiimidazole (CDI), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC.HCl), O-(1H-benzotriazol-1-yl)-1,1,3,3-tetramethyl-uronium tetrahydroborate (TBTU), O-(1H-benzotriazol-1-yl)-N,N,N′,N′-tetramethyl-uronium hexafluorophosphate (HBTU), or (benzotriazol-1-yloxy)tris(dimethylamino)phosphonium hexafluorophosphate. Any solvents which may be stable under the reaction condition may be used in the step without any limitation. Specifically, the same solvents used in the acid-halide method, or aprotic polar solvents such as N,N-dimethylformamide, N,N-dimethylacetamide, N-methyl-2-pyrrolidinone, 1,3-dimethyl-2-imidazolidinone or dimethylsulfoxide, water, or a mixed solvent thereof may be used. The base includes organic bases such as triethylamine, diisopropylethylamine, tributylamine, 1,5-diazabicyclo[4.3.0]non-5-ene (DBN), 1,4-diazabicyclo[2.2.2] octane (DABCO), 1,8-diazabicyclo[5.4.0] undec-7-ene (DBU), pyridine, dimethylaminopyridine, picoline or N-methylmorpholine (NMM) without any limitation. The reaction is usually carried out at −10° C. to reflux temperature. The reaction time is usually 0.5 to 48 hours depending mainly on reaction temperatures, starting materials and solvents.

The invention encompasses the following embodiments [PC1]-[PC13].

[PC1] A process for preparing pyrazole (A-5), wherein a reaction system with a base is applied before the addition of an acid in the step in which thiosemicarbazide (A-2) is treated with alpha-halo ketoester (A-3) to give pyrazole (A-5) with or without isolating Compound (A-4).

[PC2] The process for preparing of [PC1], wherein the base added in the reaction is an inorganic base.

[PC3] The process for preparing of [PC1], wherein the inorganic base added in the reaction is one or more combinations selected from sodium bicarbonate, potassium bicarbonate, lithium bicarbonate, sodium carbonate, potassium carbonate or lithium carbonate.

[PC4] A process for preparing pyrazole (A-5), wherein the reaction system contains water before the addition of an acid in the step in which thiosemicarbazide (A-2) is treated with alpha-halo ketoester (A-3) to give pyrazole (A-5) with or without isolating Compound (A-4).

[PC5] A process for preparing pyrazole (A-5), wherein the reaction system is concentrated before the addition of an acid in the step in which thiosemicarbazide (A-2) is treated with alpha-halo ketoester (A-3) to give pyrazole (A-5) with or without isolating Compound (A-4).

[PC6] A process for preparing pyrazole (A-5), wherein the reaction is carried out with removing solvents from the reaction system after the addition of an acid in the steps in which thiosemicarbazide (A-2) is treated with alpha-halo ketoester (A-3) to give pyrazole (A-5).

[PC7] A process for preparing pyrazole (A-5), wherein the reaction is carried out with evaporating solvents from the reaction system after the addition of an acid in the steps in which thiosemicarbazide (A-2) is treated with alpha-halo ketoester (A-3) to give pyrazole (A-5).

[PC8] A process for preparing pyrazole (A-5), wherein the added acid is an organic acid or inorganic acid in the step in which thiosemicarbazide (A-2) is treated with alpha-halo ketoester (A-3) to give pyrazole (A-5) with or without isolating Compound (A-4).

[PC9] The process for preparing of [PC8], wherein the added acid is one or more combinations selected from hydrochloric acid, hydrobromic acid, sulfuric acid, propionic acid, acetic acid, formic acid, methanesulfonic acid, toluenesulfonic acid or trifluoroacetic acid.

[PC10] The process for preparing of [A8], wherein the added acid is acetic acid.

[PC11] A process for preparing pyrazole (A-5), comprising one to four combinations selected from [PC1] to [PC3], [PC4], [PC5] to [PC7], [PC8] to [PC10] in the step in which thiosemicarbazide (A-2) is treated with alpha-halo ketoester (A-3) to give pyrazole (A-5) with or without isolating Compound (A-4).

[PC12] A process for preparing pyrazole (A-5), comprising a combination selected from [PC3], [PC4], [PC5] or [PC7], and [PC10] in the steps in which thiosemicarbazide (A-2) is treated with alpha-halo ketoester (A-3) to give pyrazole (A-5) with or without isolating Compound (A-4).

[PC13] A process for preparing pyrazole (A-5) of [PC11] or [PC12], wherein R_Aand/or R_Bof thiosemicarbazide (A-2) contain the same or different one or more groups selected from Cbz, Boc, tetrahydrofuranyl, tetrahydropyranyl, cyclopropyl, cyclobutyl, optionally substituted benzyloxy or optionally substituted benzylamino as a partial structure.

Preparation 2

A compound of formula (A-12) or a salt thereof among a compound of formula (1) is, for example, prepared according to the following methods.

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(In the above scheme, R_A, R_B, R_C, R_Eand R_Fare the same as defined above. R is methyl, ethyl, benzyl, etc. X is halogen atom, etc.)

Step 8:

Halogen (X) is introduced at 4-position of pyrazole ring in Compound (A-9) to give Compound (A-10) in the step.

Halogen atom may be introduced at 4-position in Compound (A-9) by adding a halogenating agent such as N-chlorosuccinimide, N-bromosuccinimide, chlorine, bromine, iodine, iodine chloride, sulfuryl chloride, SELECTFLUOR®, 1-fluoro-4-hydroxy-1,4-diazoniabicyclo[2.2.2] octane bis(tetrafluoroborate), N-fluorobenzenesulfonimide, N-fluoro-o-benzenedisulfonimide, 1-fluoropyridinium triflate or 1-fluoro-2,6-dichloropyridinium tetrafluoroborate in the presence or absence of an acid. The acid includes hydrogen halides such as hydrogen chloride or hydrogen bromide, or organic acids such as acetic acid or propionic acid. The reaction may be also carried out using a base instead of an acid. The base includes inorganic bases such as sodium bicarbonate, potassium bicarbonate, sodium carbonate or potassium carbonate. Any solvents which may be inert under the reaction condition may be used in the step, e.g., ester type solvents such as ethyl acetate or methyl acetate, halogenated hydrocarbon solvents such as dichloromethane, chloroform, 1,2-dichloroethane or carbon tetrachloride, aprotic polar solvents such as N,N-dimethylformamide, N,N-dimethylacetamide, N-methyl-2-pyrrolidinone or 1,3-dimethyl-2-imidazolidinone, water, or a mixed solvent thereof. The reaction temperature is usually in the range of −10° C. to reflux temperature. The reaction time is usually in the range of 0.5 to 48 hours.

Compound (A-10) may be treated by Steps 9-10 of the similar method to Preparation 1 to give Compound (A-12).

Compound (A-9) may be treated by Steps 11-12 of the similar method to Preparation 1 to give Compound (A-14).

Compound (A-14) may be treated by the similar method to Step 8 to give Compound (A-12).

Preparation 3

A compound of formula (A-17) or a salt thereof among a compound of formula (1) is prepared according to the following method.

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(In the above scheme, R_B, R_C, R_D, R_E, R_Fand p are the same as defined above. Pro is a protective group of nitrogen atom. B³is acyl or sulfonyl.)

Step 14:

Compound (A-15) wherein Pro is benzyloxycarbonyl may be treated in the following manner to give Compound (A-16). Compound (A-15) may be treated with hydrogen in an inert solvent usually at ambient temperature to 50° C. for 0.5 to 24 hours in the presence of palladium/carbon to give Compound (A-16). Hydrogen may be used at normal pressure or with pressurized. The inert solvent includes halogenated hydrocarbon solvents such as dichloromethane, chloroform, 1,2-dichloroethane or carbon tetrachloride, ether type solvents such as diethylether, diisopropylether, tetrahydrofuran or 1,4-dioxane, aromatic hydrocarbon solvents such as benzene, toluene or xylene, ester type solvents such as ethyl acetate or methyl acetate, water, or a mixed solvent thereof. Ammonium formate may be used instead of hydrogen.

Step 15:

Acylation or sulfonylation of a deprotected amine of Compound (A-16) may give Compound (A-17) in the step.

The acylation may be carried out in the similar manner to Step 7 of Preparation 1 by using acid halide or carboxylic acid compound to give Compound (A-17) as an amide derivative.

The sulfonylation may be carried out in the similar manner to the acid-halide method of Step 7 of Preparation 1 by using sulfonyl halide such as arylsulfonyl halide to give Compound (A-17) as a sulfoneamide derivative.

Preparation 4

A compound of formula (A-18) or a salt thereof among a compound of formula (1) is prepared by the following method.

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(In the above scheme, R_B, R_C, R_D, R_E, R_Fand p are the same as defined above. R_Gand R_Hare each hydrogen atom, optionally substituted alkyl, optionally substituted aryl, optionally substituted heteroaryl, optionally substituted cycloalkyl or optionally substituted heterocycloalkyl. Alternatively, R_Gand R_Hmay combine each other together with the adjacent nitrogen atom to form an optionally substituted saturated heterocycle.)

Compound (A-16) is treated with amine R_GR_HNH or a salt thereof to give Compound (A-18) in the step. Amine R_GR_HNH is reacted with 1,1′-carbonyldiimidazole, triphosgene, diphosgene or phosgene in an inert solvent usually at −10° C. to 30° C. for 0.5 to 6 hours, followed by reacting with Compound (A-16) at −10° C. to reflux temperature for 0.5 to 8 hours. Compound (A-16) may be also treated earlier than amine R_GR_HNH. Consequently, Compound (A-18) may be prepared in this manner. Amine R_GR_HNH may be also reacted with para-nitrophenyl chloroformate or trichloromethyl chloroformate in the presence of a base in an inert solvent usually at −10° C. to 30° C., followed by reacting with Compound (A-16) usually at −10° C. to reflux temperature to give Compound (A-18). Compound (A-16) may be also treated earlier than amine R_GR_HNH. The base includes nitrogen-containing organic bases such as triethylamine, diisopropylethylamine, tributylamine, 1,5-diazabicyclo[4.3.0]non-5-ene (DBN), 1,4-diazabicyclo[2.2.2]octane (DABCO), 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU), pyridine, dimethylaminopyridine, picoline or N-methylmorpholine (NMM), or inorganic bases such as potassium carbonate, sodium carbonate or sodium bicarbonate.

The inert solvent includes ether type solvents such as tetrahydrofuran, diethylether, dioxane or 1,2-dimethoxyethane, hydrocarbons such as toluene or benzene, halogenated hydrocarbon solvents such as dichloromethane, chloroform or 1,2-dichloroethane, aprotic polar solvents such as dimethylsulfoxide, N,N-dimethylformamide, N,N-dimethylacetamide, N-methyl-2-pyrrolidinone or 1,3-dimethyl-2-imidazolidinone, a mixed solvent thereof, or a mixed solvent of these solvents with water.

Compound (A-16) may be also treated with isocyanate R_GNCO, wherein R_Gis not hydrogen atom, to give Compound (A-18).

Compound (A-16) may be treated with isocyanate R_GNCO usually at −10° C. to reflux temperature in an inert solvent or neat in the presence or absence of a base to give Compound (A-18). The base includes nitrogen-containing organic bases such as triethylamine, diisopropylethylamine, tributylamine, 1,5-diazabicyclo[4.3.0]non-5-ene (DBN), 1,4-diazabicyclo[2.2.2]octane (DABCO), 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU), pyridine, dimethylaminopyridine, picoline or N-methylmorpholine (NMM), or inorganic bases such as potassium carbonate, sodium carbonate or sodium bicarbonate. The inert solvent includes ether type solvents such as tetrahydrofuran, diethylether, dioxane or 1,2-dimethoxyethane, hydrocarbons such as toluene or benzene, halogenated hydrocarbon solvents such as dichloromethane, chloroform or 1,2-dichloroethane, aprotic polar solvents such as dimethylsulfoxide, N,N-dimethylformamide, N,N-dimethylacetamide, N-methyl-2-pyrrolidinone or 1,3-dimethyl-2-imidazolidinone, a mixed solvent thereof, or a mixed solvent of these solvents with water.

Preparation 5

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(In the above scheme, R_B, R_C, R_D, R_E, R_Fand p are the same as defined above. B⁴is aryl or heteroaryl.)

Compound (A-16) may be treated with halogenated aryl or halogenated heteroaryl (B⁴—Br, B⁴—I, B⁴—Cl, etc.) or aryl metal compound or heteroaryl metal compound (B⁴-Mtl) to give Compound (A-19), in which -Mtl is a boronic acid group —B(OH)₂, —B(OMe)₂as a boronic acid ester group, —ZnCl as a zinc halide group, etc.

Compound (A-16) may be treated with halogenated aryl, halogenated heteroaryl, aryl metal compound or heteroaryl metal compound usually at room temperature to reflux temperature in the presence or absence of a palladium, copper or nickel metal catalyst such as tetrakis(triphenylphosphine)palladium, dichlorodi(tris-o-tolylphosphine)palladium, tris(dibenzylidene-acetone)dipalladium, copper acetate, copper iodide, nickel di(cyclooctadienyl) or nickel-carbon in the presence of a base such as sodium tertiary-butoxide, potassium carbonate, sodium bicarbonate or lithium hexamethyldisilazide, or a phosphorus ligand such as 2,2′-bis(diphenylphosphino)-1,1′-binaphthyl or triphenylphosphine, if needed, in an inert solvent or neat to give Compound (A-19). The solvent includes ether type solvents such as diethylether, diisopropylether, 1,2-dimethoxyethane, tetrahydrofuran or 1,4-dioxane, aromatic hydrocarbon solvents such as benzene, toluene or xylene, aprotic polar solvents such as dimethylsulfoxide, N,N-dimethylformamide, N,N-dimethylacetamide, N-methyl-2-pyrrolidinone or 1,3-dimethyl-2-imidazolidinone, water, or a mixture thereof. The reaction time is usually in the range of 30 minutes to 48 hours.

If any functional groups except for the intended reaction sites may be affected under the reaction conditions or be inappropriate to carry out the reactions in the above Preparations, such groups except for the intended reaction sites may be protected to carry out the reactions, followed by deprotecting to give the desired compounds. The protective group includes conventional protective groups described in the Protective Groups in Organic Synthesis as mentioned above, and specifically, the protective group for amine includes ethoxycarbonyl, t-butoxycarbonyl, acetyl or benzyl, and that of hydroxyl includes tri-lower alkyl silyl, acetyl or benzyl.

An introduction or deprotection of a protective group may be carried out according to a conventional method in the organic synthetic chemistry (see, for example, the Protective Groups in Organic Synthesis), or with some modification thereof.

Any functional groups of any intermediates or final products may be also optionally modified to give other compounds encompassed in the invention in the above Preparations. The modification of functional groups may be carried out by a conventional method (see, for example, R. C. Larock, Comprehensive Organic Transformations, 1989).

Each intermediate and the desired compound may be isolated and/or purified by a conventional purification method in the organic synthetic chemistry, e.g. neutralization, filtration, extraction, washing, drying, concentration, recrystallization, various chromatography, etc., in each Preparation. Each intermediate may be also used in the next reaction without purification.

Any optical isomers may be isolated in any steps in the above Preparations by a conventional isolating method including a method using an optically-active column or a fractionated crystallization. Any optically-active starting materials may be also used in the Preparations.

The invention encompasses any possible isomers including optical isomers, stereoisomers, tautomers such as ketoenol, and/or geometrical isomers, and a mixture thereof.

Any starting materials and intermediates may be known compounds, or be synthesized therefrom by a conventional method in the Preparations.

A configuration of two substituents on adamantane group in the inventive compound is defined as Z or E relative configuration according to C. D. Jones, M. Kaselj, et al. J. Org. Chem. 63: 2758-2760, 1998.

The invention is illustrated by the following Reference Examples, Examples and Test Examples in more detail, but is not limited thereto. Compound names do not necessarily follow IUPAC nomenclature in the following Reference Examples and Examples.

The following abbreviations may be used in the Reference Examples and Examples.

THF: tetrahydrofuran

NaBH(OAc)₃: sodium triacetoxyborohydride

(Boc)₂O: di-tert-butyldicarbonate

Pd(OH)₂: palladium hydroxide

DMF: N,N-dimethylformamide

WSC.HCl: 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride

HOBt.H₂O: 1-hydroxybenzotriazole monohydrate

NMP: 1-methyl-2-pyrrolidinone

Me: methyl

Et: ethyl

Boc: tert-butoxycarbonyl

Cbz: benzyloxycarbonyl

N: normal (e.g., 2N HCl is 2-normal hydrochloric acid.)

M: molar concentration (mol/L) (e.g., 2M methylamine is 2 mol/L methylamine solution.)

t_R: retention time

A reverse-phase preparative purification was carried out as below.

A purification was carried out by using Gilson HPLC System. YMC CombiPrep ODS-A column (5 μm, 50×20 min I.D.) was used, and a mixed solvent system of CH₃CN (containing 0.035% TFA) with water (containing 0.05% TFA) was used. UV was detected in each wavelength of 210 nm, 220 nm and 254 nm

Elution conditions were as follows.

Preparative instrument: Gilson HPLC System

Column: YMC CombiPrep ODS-A 50×20 min I.D.

Solvent: CH₃CN (containing 0.035% TFA), water (containing 0.05% TFA)

Flow rate: 35 mL/min

Gradient: linear gradient from 1:99 (v/v) CH₃CN/water to 95:5 (v/v) CH₃CN/water within 13 min at 35 mL/min

obsMS [M+1]: observed protonated molecules

min: minute

LC/MS analytic conditions for identifying compounds in reverse-phase preparative purifications were as follows.

Measurement method SA:

Detection device: Detector Perkin-Elmer Sciex API150EX Mass spectrometer (40 eV)

HPLC: Shimadzu LC 10ATVP
Column: Shiseido CAPCELL PAK C18 ACR(S-5 um, 4.6×50 mm)
Solvent: Solution A: 0.35% TFA/CH₃CN, Solution B: 0.05% TFA/H₂O

Gradient condition: 0.0-0.5 min A 10%, 0.5-4.8 min Linear gradient from A 10% to 99%, 4.8-5.0 min A 99%

Flow rate: 3.5 mL/min

UV: 254 nm

Measurement method SB:

Detection device: Agilent 1100 series for API series, manufactured by Applied Biosystems

HPLC: API150EX LC/MS system, manufactured by Applied Biosystems

Column: YMC CombiScreen ODS-A (S-5 μm, 12 nm, 4.6×50 mm)
Solvent: Solution A: 0.05% TFA/H₂O, Solution B: 0.035% TFA/MeCN

Gradient condition: 0.0-0.5 min A 90%, 0.5-4.2 min Linear gradient from A 90% to 1%, 4.2-4.4 min

Linear gradient from A 1% to 99%

Flow rate: 3.5 mL/min

UV: 220 nm
EXAMPLES
Reference Example 1
Methyl 4-aminoadamantane-1-carboxylate hydrochloride

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Step (i):

To a solution of Compound I (40.0 g) (see The Journal of Organic Chemistry, 1983, Vol. 48, page 1099) in methanol (500 mL) was added thionyl chloride (22.7 mL). The mixture was heated to reflux and stirred for 3 hours. Then, the mixture was concentrated in vacuo, and then extracted with saturated sodium bicarbonate water and ethyl acetate. The organic layer was dried over sodium sulfate and concentrated in vacuo to give Compound II (44.0 g).

Step (ii):

Compound II (55.4 g) was dissolved in dichloromethane (1.25 L), and thereto were added (R)-(+)-1-phenetylamine (32.2 g), NaBH(OAc)₃(82.0 g) and acetic acid (10 mL). The mixture was stirred at room temperature overnight. The mixture was treated with 6N hydrochloric acid, and then basified by 2N sodium hydroxide solution and extracted with chloroform. The organic layer was dried over sodium sulfate and concentrated in vacuo. The residue was purified by silica gel column chromatography (eluent: chloroform/meththanol=100/0 to 98/2) to give Compound III (73.6 g).

Step (iii):

To a solution of Compound III (12.6 g) in acetic acid (200 mL) was added palladium hydroxide (6.0 g), and the mixture was stirred under hydrogen (3 atm) for 9 hours. The palladium was filtered off, and then the filtrate was concentrated in vacuo. The residue was dissolved in saturated sodium bicarbonate water and THF, and thereto was added (Boc)₂O (9.65 g). The mixture was stirred at room temperature for 1.5 hours. The reaction solution was extracted with ethyl acetate and saturated sodium bicarbonate water. The organic layer was dried over sodium sulfate and concentrated in vacuo. The residue was purified by silica gel column chromatography (eluent: chloroform/meththanol=19/1), and dissolved in chloroform (150 mL). Then, thereto was added 4N hydrochloric acid-dioxane (50 mL), and the mixture was stirred at room temperature overnight. The mixture was concentrated in vacuo, and the resulting white solid was filtered and concentrated in vacuo to give the titled Compound IV (7.0 g).

¹H-NMR (DMSO-d₆) δ 1.50 (m, 1H), 1.70-1.80 (m, 4H), 1.87-2.06 (m, 6H), 2.06-2.10 (m, 3H), 3.31 (s, 3H), 8.17 (bs, 3H)

Reference Example 2
5-(Dimethylamino)-1-methyl-1H-pyrazole-3-carboxylic acid

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Step (i):

To a solution of Compound I (20.0 g) in THF (400 mL) was added dropwise 2N dimethylamine-THF solution (56 mL), and the mixture was stirred at room temperature for 2 hours. Then, thereto was added dropwise hydrazine monohydrate (24 mL), and the mixture was stirred under reflux for 3 hours. The mixture was concentrated in vacuo, and then thereto was added saturated sodium bicarbonate water. The mixture was extracted with ethyl acetate, and the organic layer was dried over sodium sulfate and concentrated in vacuo to give Compound II (21.0 g).

Step (ii):

Compound II was dissolved in a mixed solvent of ethanol (100 mL) and THF (200 mL), and thereto were added sodium bicarbonate (16.2 g) and ethyl bromopyruvate (38.4 g). The mixture was stirred at 60° C. for 3 hours. Then, thereto was added 4N hydrochloric acid-dioxane (50 mL), and the mixture was stirred at 70° C. for 3 hours. The mixture was concentrated in vacuo, and then thereto was added saturated sodium bicarbonate water. The mixture was extracted with ethyl acetate. The organic layer was dried over sodium sulfate and concentrated in vacuo to give Compound III (21.0 g).

Step (iii):

Compound III (21.0 g) was dissolved in THF (1 L), and thereto was added sodium hydride (5.8 g) at 0° C. The mixture was stirred at 0° C. to room temperature for 1 hour. Then, thereto was added methyl iodide (8.2 mL) at 0° C., and the mixture was stirred at room temperature overnight. Thereto was added water, and then the mixture was concentrated in vacuo. Thereto was added saturated sodium bicarbonate water, and the mixture was extracted with ethyl acetate. The organic layer was dried over sodium sulfate and concentrated in vacuo. The residue was purified by silica gel chromatography (eluent: hexane/ethyl acetate=1/1) to give Compound IV (6.5 g).

Step (iv):

Compound IV (6.5 g) was dissolved in methanol (300 mL), and thereto was added 2N sodium hydroxide solution (30 mL) and the mixture was stirred at room temperature overnight. The reaction solution was concentrated in vacuo, and then acidified by 1N hydrochloric acid and extracted with ethyl acetate. The organic layer was dried over sodium sulfate and concentrated in vacuo to give Compound V (4.4 g) as a yellow oil.

¹H-NMR (DMSO-d₆) δ 2.62 (s, 6H), 3.69 (s, 3H), 6.21 (s, 1H), 12.4 (bs, 1H)

Reference Example 3
(E)-4-Aminoadamantan-1-ol hydrochloride

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Step (i):

To a solution of 5-hydroxy-2-adamantanone (10.0 g) in dichloromethane (200 mL) were added (S)-(−)-1-phenetylamine (7.2 g), NaBH(OAc)₃(19 g) and acetic acid (2 mL), and the mixture was stirred at room temperature for 4 hours. Thereto was added 1N hydrochloric acid, and the mixture was washed with chloroform, and then the aqueous layer was basified by 2N sodium hydroxide solution. The mixture was extracted with chloroform, dried over sodium sulfate, and concentrated in vacuo. The residue was purified by silica gel chromatography (eluent: chloroform/meththanol=10/1) to give Compound II (5.9 g) as a low-polar ingredient and Compound III (4.2 g) as a high-polar one.

Step (ii):

Compound II (5.9 g) was dissolved in acetic acid (80 mL), and thereto was added palladium hydroxide (3.0 g) and the mixture was stirred under hydrogen (3 atm) for 8.5 hours. The resulting solid was filtered through Celite®, and then the filtrate was concentrated. The residue was dissolved in THF (100 mL) and saturated sodium bicarbonate water (50 mL), and thereto was added (Boc)₂O (4.7 g) and the mixture was stirred at room temperature for 4 hours. The reaction solution was extracted with ethyl acetate. The organic layer was dried over sodium sulfate and concentrated in vacuo. The residue was purified by silica gel chromatography (eluent: hexane/ethyl acetate=1/1) and dissolved in chloroform (100 mL), and thereto was added 4N hydrochloric acid-dioxane (20 mL) and the mixture was stirred at room temperature for 3 hours. The mixture was concentrated in vacuo and azeotroped with toluene to give Compound IV (4.9 g) as a white solid.

¹H-NMR (DMSO-d₆) δ 1.35-1.39 (m, 2H), 1.59-1.69 (m, 7H), 1.86-1.90 (m, 2H), 2.01 (m, 1H), 2.06-2.12 (m, 2H), 4.50 (bs, 1H), 8.07 (bs, 3H)

Reference Example 4
N-(3-Methoxybenzyl)cyclopropaneamine

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Cyclopropaneamine (1.5 g) was dissolved in dichloromethane (50 mL), and thereto were added 3-methoxybenzaldehyde (3.5 g), NaBH(OAc)₃(6.7 g) and acetic acid (1 mL) and the mixture was stirred at room temperature overnight. Thereto was added water, and then the mixture was extracted with chloroform. The organic layer was dried over sodium sulfate and concentrated in vacuo. The residue was purified by silica gel chromatography (eluent: chloroform/methanol=10/1) to give Compound II (2.8 g) as a colorless oil.

¹H-NMR (CDCl₃) δ 0.36-0.41 (m, 2H), 0.43-0.47 (m, 2H), 2.17 (m, 1H), 3.82 (s, 3H), 3.83 (s, 2H), 6.80 (m, 1H), 6.87-6.92 (m, 2H), 7.24 (m, 1H)

Reference Example 5
Ethyl-5-[cyclopropyl(3-methoxybenzyl)amino]-1-methyl-1H-pyrazole-3-carboxylate

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Step (i):

To a solution of Compound I (2.8 g) in THF (150 mL) was added dropwise N-(3-methoxybenzyl)cyclopropaneamine (2.8 g), and the mixture was stirred at room temperature for 5 hours. Then, thereto was added dropwise hydrazine monohydrate (8 mL), and the mixture was stirred under reflux for 6 hours. The mixture was concentrated in vacuo, and then thereto was added saturated sodium bicarbonate water and the mixture was extracted with ethyl acetate. The organic layer was dried over sodium sulfate and concentrated in vacuo. The resulting white solid was filtered, washed with water and dried in vacuo to give Compound II (3.5 g).

Step (ii):

Compound II (1.3 g) was dissolved in ethanol (10 mL) and THF (10 mL), and thereto were added sodium bicarbonate (0.54 g) and ethyl bromopyruvate (1.5 g) and the mixture was stirred at 70° C. for 3 hours. Then, thereto was added acetic acid (1.2 mL) and the mixture was stirred at 60° C. for 4.5 hours. The mixture was concentrated in vacuo, and thereto was added saturated sodium bicarbonate water and the mixture was extracted with ethyl acetate. The organic layer was dried over sodium sulfate and concentrated in vacuo to give Compound III (62 mg).

Step (iii):

Compound III (62 mg) was dissolved in THF (5 mL), and thereto was added sodium hydride (9.4 mg) at 0° C. The mixture was stirred at 0° C. to room temperature for 1 hour. Then, thereto was added methyl iodide (13 μL) at 0° C. and the mixture was stirred at room temperature for 3 hours. Thereto was added water, then saturated sodium bicarbonate water, and the mixture was extracted with ethyl acetate. The organic layer was dried over sodium sulfate and concentrated in vacuo. The residue was purified by preparative TLC (eluent: hexane/ethyl acetate=1/1) to give Compound IV (24 mg).

¹H-NMR (CDCl₃) δ 0.71-0.74 (m, 2H), 0.76-0.80 (m, 2H), 1.38 (t, J=7.12 Hz, 3H), 2.54 (m, 1H), 3.58 (s, 3H), 3.78 (s, 3H), 4.36 (q, J=7.12 Hz, 2H), 4.53 (s, 2H), 6.43 (s, 1H), 6.76-6.85 (m, 3H), 7.20 (m, 1H)

Reference Example 6
N-[2-(4-Fluorophenoxy)ethyl]-N-methylhydrazinecarbothioamide

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Step (i):

Compound I (41.3 g) was dissolved in THF (100 mL), and thereto were added saturated sodium bicarbonate water (100 mL) and (Boc)₂O (120 g) and the mixture was stirred at room temperature overnight. Thereto was added water, and the mixture was extracted with ethyl acetate. The organic layer was dried over sodium sulfate and then concentrated in vacuo to give Compound II (94.4 g).

Step (ii):

Compound II (5.0 g) was dissolved in THF (200 mL), and thereto were added 4-fluorophenol (3.2 g) and triphenylphosphine (7.5 g), then added dropwise diisopropyl azodicarboxylate (5.5 g). The mixture was stirred at room temperature overnight and the reaction solvent was concentrated in vacuo. The residue was purified by silica gel chromatography (eluent: hexane/ethyl acetate=3/1) to give Compound III (2.3 g).

Step (iii):

Compound III (2.3 g) was dissolved in chloroform (100 mL), and thereto was added 4N hydrochloric acid-dioxane solution (30 mL) and the mixture was stirred at room temperature for 6 hours. The reaction solvent was concentrated in vacuo, and thereto was added 2N sodium hydroxide solution and the mixture was extracted with chloroform. The organic layer was dried over sodium sulfate and then concentrated in vacuo to give Compound IV (1.8 g).

Step (iv):

1,1′-Thiocarbonyl diimidazole (2.0 g) was dissolved in THF (70 mL), and thereto was added Compound IV (1.8 g) and the mixture was stirred at room temperature for 1 hour. Then, thereto was added hydrazine monohydrate (10 mL) and the mixture was stirred under reflux for 1 hour. The mixture was concentrated in vacuo, and thereto was added water and the mixture was extractd with ethyl acetate. The organic layer was dried over sodium sulfate and concentrated in vacuo to give the titled Compound V (1.8 g).

¹H-NMR (CDCl₃) δ 1.24 (t, J=8.0 Hz, 2H), 2.03 (s, 3H), 4.10 (q, J=8.0 Hz, 2H), 4.20 (m, 1H), 6.56-6.66 (m, 4H), 7.12-7.14 (m, 2H)

Reference Example 7
4-Chloro-5-[cyclobutyl(2,2,2-trifluoroethyl)amino]-1-methyl-1H-pyrazole-3-carboxylic acid

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Step (i):

Cyclobutylamine (7.1 g) was dissolved in dichloromethane (400 mL), and thereto was added anhydrous trifluoroacetic acid (17 mL) and the mixture was stirred at room temperature overnight. The mixture was concentrated in vacuo to give Compound II (10.5 g).

Step (ii):

To a solution of borane-dimethyl sulfide complex (21.5 g) in THF (300 mL) was added dropwise a solution of Compound II (10.5 g) in THF (50 mL) at 50° C., and the mixture was stirred at 50° C. overnight. Thereto was added methanol (150 mL) at 0° C., and the mixture was stirred at room temperature for 1 hour. Then, thereto was added 4N hydrochloric acid-ethanol solution (100 mL) and the mixture was stirred at room temperature for 1 hour. The reaction solution was concentrated in vacuo, and the residue was washed with ethyl acetate-hexane. The resulting white solid was filtered and dried in vacuo to give Compound III (10.9 g).

Step (iii):

Compound III (1.9 g) was dissolved in THF (20 mL), and thereto was added saturated sodium bicarbonate water (10 mL) and the mixture was stirred at room temperature for 30 minutes. Thereto was added dropwise a solution of 4-chlorophenyl chlorothioformate (2.3 g) in THF (5 mL) at 0° C., and the mixture was stirred at room temperature for 4 hours. Thereto was added brine, and the mixture was extracted with ethyl acetate. The organic layer was dried over sodium sulfate, concentrated in vacuo and dissolved in NMP (12 mL), and thereto was added hydrazine monohydrate (1.5 mL) and the mixture was stirred at room temperature for 1 hour. Thereto was added water, and then the mixture was extracted with ethyl acetate. The organic layer was washed with brine, and then dried over sodium sulfate and concentrated in vacuo. The residue was purified by silica gel chromatography (eluent: hexane/ethyl acetate=1/1) to give Compound IV (2.0 g).

Step (iv):

Compound IV (2.0 g) was dissolved in a mixed solvent of ethanol (20 mL) with THF (20 mL), and thereto were added sodium bicarbonate (765 mg) and ethyl bromopyruvate (1.8 g) and the mixture was stirred at 80° C. for 3 hours. Thereto was added 4N hydrochloric acid-ethanol solution (3 mL), and the mixture was stirred at 60° C. for 12 hours. The mixture was concentrated in vacuo, and then thereto was added saturated sodium bicarbonate water and the mixture was extracted with ethyl acetate. The organic layer was dried over sodium sulfate and concentrated in vacuo. The residue was purified by silica gel chromatography (eluent: hexane/ethyl acetate=1/1) to give Compound V (780 mg).

Step (v):

To a solution of sodium hydride (140 mg) in THF (10 mL) was added dropwise a solution of Compound V (778 mg) in THF (5 mL) at 0° C., and the mixture was stirred at room temperature for 1 hour. Then, thereto was slowly added methyl iodide (200 μL) at 0° C., and the mixture was stirred at room temperature overnight. Thereto was added water, and then the mixture was concentrated in vacuo. Thereto was added brine, and the mixture was extracted with ethyl acetate. The organic layer was dried over sodium sulfate and concentrated in vacuo. The residue was purified by silica gel chromatography (eluent: hexane/ethyl acetate=1/1) to give Compound VI (590 mg).

Step (vi):

Compound VI (340 mg) was dissolved in DMF (4.5 mL), and thereto was added N-chlorosuccinimide (178 mg) and the mixture was stirred at 60° C. for 4 hours. Thereto was added water, and then the mixture was extracted with ethyl acetate. The organic layer was washed with brine. The organic layer was dried over sodium sulfate and concentrated in vacuo. The residue was purified by silica gel chromatography (eluent: hexane/ethyl acetate=3/1) to give Compound VII (324 mg).

Step (vii):

Compound VII (320 mg) was dissolved in ethanol (4 mL), and thereto was added 5N sodium hydroxide solution (560 μL) and the mixture was stirred at room temperature overnight. The reaction solution was concentrated in vacuo, and then acidified by 1N hydrochloric acid and extracted with ethyl acetate. The organic layer was dried over sodium sulfate and concentrated in vacuo to give the titled Compound VIII (287 mg) as a white solid.

¹H-NMR (CDCl₃) δ 1.51-1.69 (m, 2H), 1.75-1.88 (m, 2H), 2.05-2.20 (m, 2H), 3.52-3.80 (m, 2H), 3.83 (s, 3H), 3.84-3.95 (m, 2H)

Reference Example 8
Ethyl 1,4-dimethyl-5-[methyl(2,2,2-trifluoroethyl)amino]-1H-pyrazole-3-carboxylate

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Step (i):

To a solution of borane-dimethyl sulfide complex (23.9 g) in THF (300 mL) was added dropwise a solution of Compound I (10.0 g) in THF (50 mL) at 50° C. and the mixture was stirred at 50° C. overnight. Thereto was added methanol (150 mL) at 0° C., and the mixture was stirred at room temperature for 1 hour. Then, thereto was added 4N hydrochloric acid-ethanol solution (100 mL) and the mixture was stirred at room temperature for 1 hour. The reaction solution was concentrated in vacuo, and the residue was washed with ethyl acetate-hexane. The resulting white solid was filtered and dried in vacuo to give Compound II (10.0 g).

Step (ii):

Compound II (5.5 g) was dissolved in THF (40 mL), and thereto was added triethylamine (5.1 mL) and the mixture was stirred at room temperature for 30 minutes. The mixture was added to a solution of 1,1′-thiocarbonyl diimidazole (7.6 g) in THF (40 mL) and stirred at room temperature for 1 hour. Then, thereto was added hydrazine monohydrate (5.4 mL) and the mixture was stirred at room temperature for 1 hour. The mixture was concentrated in vacuo, and thereto was added brine and the mixture was extracted with ethyl acetate. The organic layer was dried over sodium sulfate and then concentrated in vacuo to give Compound III (6.1 g).

Step (iii):

Compound III (1.4 g) was dissolved in a mixed solvent of ethanol (20 mL) with THF (20 mL), and thereto were added sodium bicarbonate (670 mg) and ethyl 3-bromo ketobutanoate (2.2 g), and the resulting mixture was stirred at 70° C. for 3 hours. The reaction solution was concentrated in vacuo, and then thereto was added 4N hydrochloric acid-ethanol solution (5 mL) and the mixture was stirred at 90° C. overnight. The mixture was concentrated in vacuo, and then thereto was added saturated sodium bicarbonate water and the mixture was extracted with ethyl acetate. The organic layer was dried over sodium sulfate and concentrated in vacuo. The residue was purified by silica gel chromatography (eluent: hexane/ethyl acetate=2/1) to give Compound IV (1.1 g) as a white solid.

Step (iv):

To a solution of sodium hydride (155 mg) in THF (12 mL) was added dropwise a solution of Compound IV (854 mg) in THF (8 mL) at 0° C. and the mixture was stirred at room temperature for 1 hour. Then, thereto was slowly added methyl iodide (240 μL) at 0° C. and the mixture was stirred at room temperature for 3 hours. Thereto was added water, and then the mixture was concentrated in vacuo. Thereto was added brine, and the mixture was extracted with ethyl acetate. The organic layer was dried over sodium sulfate and concentrated in vacuo. The residue was purified by silica gel chromatography (eluent: hexane/ethyl acetate=2/1) to give the titled Compound V (445 mg).

¹H-NMR (CDCl₃) δ 1.40 (t, J=8.0 Hz, 3H), 2.24 (s, 3H), 2.93 (s, 3H), 3.52-3.61 (m, 2H), 3.80 (s, 3H), 4.39 (q, J=8.0 Hz, 2H)

Reference Example 9
N-(3-Methoxypropyl)cyclopropaneamine hydrochloride

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Step (i):

To an ice-cooled mixed solution of cyclopropylamine (5.0 g), saturated sodium bicarbonate water (20 mL) and THF (200 mL) was added (Boc)₂O (19.1 g), and the mixture was stirred at room temperature overnight. The organic layer was separated and the aqueous layer was extracted with ethyl acetate. The organic layer was dried over sodium sulfate and concentrated in vacuo to give Compound II (13.0 g).

Step (ii):

To an ice-cooled mixed solution of sodium hydride (1.7 g) and DMF (70 mL) was added dropwise a solution of Compound II (5.0 g) in DMF (5 mL). The mixture was stirred at room temperature for 1 hour and cooled to 0° C. again. Thereto was added dropwise 1-bromo-3-methoxypropane (7.3 g), and then the mixture was stirred at room temperature overnight. Thereto was added water, and the mixture was extracted with ethyl acetate. The organic layer was dried over sodium sulfate and concentrated in vacuo to give Compound III.

Step (iii):

Compound III obtained in Step (ii) was dissolved in dioxane (25 mL), and thereto was added 4N hydrochloric acid-dioxane solution (25 mL) and the mixture was stirred at room temperature for 4 hours. The mixture was concentrated in vacuo, and the residue was washed with dioxane and hexane to give the titled Compound IV (4.3 g).

¹H-NMR (CDCl₃) δ 0.67-0.74 (m, 2H), 0.82-0.86 (m, 2H), 1.82-1.88 (m, 2H), 2.64-2.69 (m, 1H), 2.97-3.05 (m, 2H), 3.23 (s, 3H), 3.33-3.39 (m, 2H), 8.92 (bs, 2H)

Reference Example 10
N-(2-Methoxyethyl)cyclopropaneamine hydrochloride

The titled compound was synthesized by using 1-bromo-2-methoxyethane in the similar manner to Reference Example 9.

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¹H-NMR (CDCl₃) δ 0.68-0.73 (m, 2H), 0.82-0.86 (m, 2H), 2.63-2.69 (m, 1H), 3.13-3.16 (m, 2H), 3.29 (s, 3H), 3.59-3.62 (m, 2H), 9.06 (bs, 2H)

Reference Example 11
N-Cyclopropylcyclopropaneamine hydrochloride

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Step (i):

To a solution of cyclopropylamine (3.0 g) and benzaldehyde (5.6 g) in methylene chloride (200 mL) was added NaBH(OAc)₃(12.3 g), and the mixture was stirred at room temperature overnight. Thereto was added water, and the aqueous layer was extracted with chloroform. The organic layer was washed with brine, dried over sodium sulfate and concentrated in vacuo. The residue was dissolved in butyl formate (100 mL) and stirred at 150° C. overnight. The mixture was concentrated in vacuo, and the residue was purified by silica gel chromatography (eluent: hexane/ethyl acetate=3/2) to give Compound II (4.4 g).

Step (ii):

To a solution of ethylmagnesium bromide (0.96M solution in THF, 60 mL) in THF (170 mL) at −70° C. was added dropwise a solution of titanium tetraisopropoxide (9.3 g) in THF (20 mL) over 3 minutes, and the mixture was stirred for 2 minutes. Then, thereto was added dropwise a solution of Compound II (4.4 g) in THF (10 mL) over 3 minutes, and the mixture was stirred for 5 minutes. The mixture was warmed up to room temperature and stirred overnight. To the reaction solution were added saturated aqueous ammonium chloride solution (150 mL) and water (50 mL), and the mixture was stirred at room temperature for 3 hours. The white precipitate was filtered, and the filtrate was adjusted to pH 10 with 2M aqueous sodium hydroxide solution and extracted with ethyl acetate. The organic layer was washed with brine, dried over sodium sulfate and concentrated in vacuo. The residue was purified by silica gel chromatography (eluent: hexane/ethyl acetate=100/1) to give Compound III (2.7 g).

Step (iii):

A mixed solution of Compound III (2.7 g), methanol (60 mL), 4N hydrochloric acid-dioxane (7.5 mL) and 10% palladium-carbon (300 mg) was stirred at room temperature under hydrogen (3 atm) for 4.5 hours. The reaction solution was filtered through Celite®, and then the filtrate was concentrated to give the titled Compound IV (2.0 g).

¹H-NMR (CDCl₃) δ 0.71-0.78 (m, 4H), 0.80-0.91 (m, 4H), 2.73 (bs, 2H), 9.35 (bs, 2H)

Reference Example 12
N-Methyl-1-(1-phenylcyclobutyl)methaneamine hydrochloride

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Step (i):

To a mixed solvent of toluene (135 mL) and water (10 mL) were added benzyl cyanide (5.9 g), potassium hydroxide (26.4 g), 1,3-dibromopropane (10.1 g) and tetrabutylammonium bromide (0.16 g), and the mixture was heated with stirring at 100° C. After dissolving potassium hydroxide, the reaction vessel was soaked in a water bath and vigorously stirred for 10 minutes. Then, the mixture was heated with stirring at 110° C. for 5 hours. Thereto was added water, and the aqueous layer was extracted with ethyl acetate. The organic layer was washed with brine, dried over sodium sulfate and concentrated in vacuo. The residue was purified by silica gel chromatography (eluent: hexane/ethyl acetate=20/1) to give Compound II (4.1 g).

Step (ii):

Lithium aluminum hydride (3.8 g) was suspended in THF (120 mL), and thereto was added dropwise a solution of Compound II in THF (5 mL) at room temperature. After completion of dropping, the reaction solution was heated at reflux for 5 hours. Thereto were added water (4 mL), aqueous sodium hydroxide solution (15%, 4 mL) and water (12 mL) under ice cooling, and the resulting precipitate was filtered off. The organic layer was concentrated in vacuo. To the residue was added water, and the mixture was extracted with ethyl acetate. The organic layer was washed with brine, dried over sodium sulfate and concentrated in vacuo. The residue was dissolved in ethyl formate (50 mL) and stirred at 100° C. overnight. The mixture was concentrated in vacuo, and then the residue was dissolved in THF (20 mL) and the solution was added dropwise to a suspension of lithium aluminum hydride (3.8 g) in THF (120 mL) at room temperature. After completion of dropping, the mixture was heated at reflux for 1 hour and stirred at room temperature overnight. Thereto was added sodium sulfate decahydrate until ceasing of gas generation, and then thereto was added anhydrous sodium sulfate. The precipitate was filtered off and the filtrate was concentrated in vacuo to give Compound III (4.7 g).

¹H-NMR (CDCl₃) δ 1.72-1.82 (m, 1H), 2.01-2.13 (m, 1H), 2.26-2.40 (m, 4H), 2.43-2.45 (m, 3H), 3.28-3.31 (m, 2H), 7.22-7.30 (m, 3H), 7.36-7.39 (m, 2H), 8.23 (bs, 2H)

Reference Example 13
Benzyl 4-[[3-(ethoxycarbonyl)-1H-pyrazol-5-yl] (methyl)amino]piperidine-1-carboxylate

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Step (i):

To a solution of Compound I (7.1 g) in dichloromethane (400 mL) was added a solution of methylamine in THF (110 mL, 2M). After ice-cooling, thereto was added acetic acid (43 mL), then NaBH(OAc)₃(35.3 g) in small portions. The mixture was stirred at room temperature overnight, and thereto were added water (200 mL) and potassium carbonate. After the completion of gas generation, the organic layer was separated. The aqueous layer was extracted with dichloromethane. The organic layer was combined to be dried over sodium sulfate and concentrated in vacuo to give Compound II (quantitative).

Step (ii):

To an ice-cooled mixture of Compound II obtained in Step (i), THF (200 mL), water (100 mL) and sodium bicarbonate (19.8 g) was added dropwise a solution of 4-chlorophenyl chlorothioformate (17.6 mL) in THF (100 mL), and the mixture was stirred at room temperature for 4 hours. The organic layer was separated, and then the aqueous layer was extracted with ethyl acetate. The organic layer was combined to be dried over sodium sulfate and concentrated in vacuo. To the residue was added DMF (200 mL) with ice cooling, and then thereto was added hydrazine monohydrate (12.6 mL) and the mixture was stirred at room temperature for 2 hours. Thereto was added brine, and then the mixture was extracted with ethyl acetate. The organic layer was washed with brine, and then dried over sodium sulfate and concentrated in vacuo. The residue was purified by silica gel chromatography (eluent: hexane/ethyl acetate=1/1 to chloroform/meththanol=10/1) to give Compound III (23.75 g).

Step (iii):

To an ice-cooled mixture of Compound III (23.75 g), sodium bicarbonate (12.37 g), 95% ethanol (250 mL) and THF (100 mL) was added ethyl bromopyruvate (11.6 mL). The mixture was stirred at room temperature for 30 minutes and then stirred at 90° C. After 2 hours, thereto was added acetic acid (150 mL) and the mixture was stirred at 125° C. while removing solvents with a Dean-Stark apparatus. The mixture was stirred overnight, and then cooled to room temperature and concentrated in vacuo. To the residue was added saturated sodium bicarbonate water, and the mixture was extracted with ethyl acetate. The organic layer was dried over sodium sulfate and concentrated in vacuo. The residue was purified by silica gel chromatography (eluent: hexane/ethyl acetate=1/1 to chloroform/ethanol=10/1) to give Compound III (14.8 g).

¹H-NMR (CDCl₃) δ 1.38 (t, J=4 Hz, 3H), 1.60-1.80 (m, 4H), 2.74 (s, 3H), 2.87 (m, 2H), 3.80 (m, 1H), 4.20-4.41 (m, 4H), 5.14 (s, 2H), 6.16 (s, 1H), 7.31-7.40 (m, 5H), 9.76 (br, 1H)

Reference Example 14
Benzyl 4-{[[3-(ethoxycarbonyl)-1H-pyrazol-5-yl](methyl)amino]methyl}piperidine-1-carboxylate

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Step (i):

A solution of Compound I (5 g) in ethyl formate (8 mL) was stirred under reflux for 16 hours. The solution was concentrated in vacuo to give Compound II (quantitative). Repetitions of Step (i) gave enough amounts of Compound II for Step (ii).

Step (ii):

To an ice-cooled solution of Compound II (49.1 g) in THF (500 mL) was added dropwise borane-dimethyl sulfide complex (171 mL). After the completion of dropwise, the mixture was stirred at room temperature. After ceasing of gas generation, the mixture was stirred at 50° C. for 3 hours, and then stirred at room temperature overnight. To the ice-cooled reaction solution was added dropwise methanol (200 mL), and then the mixture was stirred at room temperature for 30 minutes and concentrated in vacuo. Then, to the residue was added water (100 mL), and the mixture was acidified with hydrochloric acid. The mixture was stirred for 2 hours, and then the resulting solid was filtered off. The filtrate was extracted with toluene twice. To the aqueous layer was added sodium hydroxide, and the mixture was adjusted to pH>12 and extracted with dichloromethane three times. The organic layer was washed with brine, and then dried over sodium sulfate and concentrated in vacuo to give Compound III (40.94 g).

Step (iii):

To an ice-cooled mixture of Compound III (40.94 g), sodium bicarbonate (42.17 g), ethyl acetate (200 mL) and water (200 mL) was added dropwise a solution of Boc₂O (73.1 g) in ethyl acetate (200 mL). After 4 hours, the organic layer was separated. The aqueous layer was extracted with ethyl acetate. The combined organic layer was washed with brine, and then dried over sodium sulfate and concentrated in vacuo. The residue was purified by silica gel chromatography (eluent: hexane/ethyl acetate=1/1 to 1/2) to give Compound IV.

Step (iv):

To Compound IV obtained in Step (iii) were added acetic acid (200 mL) and platinum oxide (PtO₂, 5 g), and the mixture was stirred under 3-4 kgf/cm²of hydrogen atmosphere overnight. The reaction mixture was filtered through Celite® and washed with methanol. The filtrate was concentrated in vacuo, and then the residue was extracted with sodium hydroxide solution and dichloromethane. The organic layer was dried over sodium sulfate and concentrated in vacuo to give Compound V (66.93 g).

Step (v):

To an ice-cooled mixture of Compound V (66.93 g), sodium carbonate (62.1 g), toluene (200 mL) and water (300 mL) was added dropwise a solution of benzyloxycarbonyl chloride (55 g) in toluene (200 mL). The mixture was stirred overnight, and then a toluene layer was separated. The aqueous layer was extracted with ethyl acetate. The combined organic layer was dried over sodium sulfate and concentrated in vacuo. To the residue was added 4N hydrochloric acid-dioxane (80 mL), and the mixture was stirred at room temperature and concentrated in vacuo. Then, thereto were added diisopropylether and hexane, and the mixture was allowed to stand overnight at room temperature. The resulting solid was filtered and washed with diisopropylether and hexane, and then dried in vacuo to give Compound VI (68.56 g).

Step (vi):

To Compound VI (25 g) was added sodium hydroxide solution, and the mixture was stirred and then extracted with dichloromethane. The organic layer was dried over sodium sulfate and concentrated in vacuo. After obtaining the free form of Compound VI, thereto was added THF (100 mL). The solution was added dropwise to an ice-cooled solution of 1,1′-thiocarbonyl diimidazole (17.3 g) in THF (300 mL). After completion of dropping, the mixture was stirred at room temperature for 1.5 hours. The reaction solution was ice-cooled, and thereto was added hydrazine monohydrate (12.6 mL) and the mixture was stirred at room temperature overnight and concentrated in vacuo. Then, the residue was extracted with ethyl acetate and brine. The organic layer was washed with brine, and then dried over sodium sulfate and concentrated in vacuo. To the residue were added diisopropylether and hexane, and the mixture was stirred for 30 minutes, and then filtered and dried in vacuo to give Compound VII (27.43 g).

Step (vii):

To an ice-cooled mixed solution of Compound VII (27.43 g), sodium bicarbonate (13.7 g), 95% ethanol (400 mL) and THF (250 mL) was added ethyl bromopyruvate (12.8 mL). The mixture was stirred for 20 minutes and then stirred at 90° C. After 1 hour, thereto was added acetic acid (250 mL), and the mixture was stirred at 125° C. while removing solvents with a Dean-Stark apparatus. The mixture was stirred overnight, and then cooled to room temperature and concentrated in vacuo. To the residue was added saturated sodium bicarbonate water, and the mixture was extracted with ethyl acetate. The organic layer was dried over sodium sulfate and concentrated in vacuo. The residue was purified by silica gel chromatography (eluent: hexane/ethyl acetate=1/1) to give the titled Compound VIII (19.07 g).

¹H-NMR (CDCl₃) δ 1.19 (m, 2H), 1.40 (t, J=8 Hz, 3H), 1.72 (m, 2H), 1.92 (m, 1H), 2.78 (m, 2H), 2.94 (s, 3H), 3.13 (d, J=12 Hz, 2H), 4.22 (m, 2H), 4.37 (q, J=8 Hz, 2H), 5.14 (s, 2H), 6.09 (s, 1H), 7.32-7.42 (m, 5H), 9.80 (br, 1H)

Reference Example 15
Benzyl 4-{2-[[3-(ethoxycarbonyl-1H-pyrazol-5-yl](methyl)amino]ethyl}piperidine-1-carboxylate

4-(2-Aminoethyl)pyridine was treated in the similar manner to Reference Example 14 to give the following compounds.

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¹H-NMR (CDCl₃) δ 1.18 (m, 2H), 1.38 (t, J=8 Hz, 3H), 1.48-1.56 (m, 3H), 1.69-1.75 (m, 2H), 2.77 (m, 2H), 2.87 (s, 3H), 3.28 (m, 2H), 4.17 (m, 2H), 4.37 (q, J=8 Hz, 2H), 5.13 (s, 2H), 6.11 (s, 1H), 7.30-7.37 (m, 5H), 9.73 (br, 1H)

Reference Example 16
Ethyl 5-[{(3R)-1-[(benzyloxy)carbonyl]pyrrolidin-3-yl}(methyl)amino]-1H-pyrazole-3-carboxylate

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Step (i):

To an ice-cooled mixed solution of Compound I (25.6 g), toluene (200 mL), sodium carbonate (32.0 g) and water (300 mL) was added dropwise a solution of benzyloxycarbonyl chloride (25.8 g) in toluene (100 mL). The mixture was stirred overnight, and then thereto was added ethyl acetate and the mixture was stirred. Then, the reaction solution was filtered and the organic layer of the filtrate was separated. The aqueous layer was extracted with ethyl acetate. The combined organic layer was washed with water, and then dried over sodium sulfate and concentrated in vacuo. To the residue were added diisopropylether and hexane, and the mixture was stirred for 20 minutes and then the resulting solid was filtered and dried in vacuo to give Compound II (41.48 g).

Step (ii):

To an ice-cooled solution of Compound II (41.48 g) in DMF (300 mL) was added sodium hydride (5.7 g) in small portions. The mixture was stirred for 1.5 hours at room temperature, and then the reaction solution was ice-cooled. Thereto was added dropwise methyl iodide (9.8 mL), and then the mixture was stirred at room temperature overnight. The reaction solution was poured into citric acid solution, and the mixture was extracted with ethyl acetate. The organic layer was washed with brine, and then dried over sodium sulfate and concentrated in vacuo. The residue was purified by silica gel chromatography (eluent: hexane/ethyl acetate=1/1). Thereto was added 15% hydrochloric acid-ethanol (100 mL), and the mixture was allowed to stand for 3 days at room temperature. The mixture was concentrated in vacuo, and then thereto was added hydrochloric acid solution and the mixture was extracted with toluene. The toluene layer was extracted with 1N hydrochloric acid. The combined acidic aqueous layer was adjusted to pH>14 with sodium hydroxide. The alkaline aqueous layer was extracted with dichloromethane, and the organic layer was dried over sodium sulfate and concentrated in vacuo to give Compound III (28.45 g).

Step (iii):

To an ice-cooled mixed solution of Compound III obtained in Step (ii), sodium bicarbonate (22.4 g), THF (100 mL) and water (100 mL) was added dropwise a solution of 4-chlorophenyl chlorothioformate (20 mL) in THF (100 mL), and the mixture was stirred at room temperature overnight. The organic layer was separated, and then the aqueous layer was extracted with ethyl acetate. The organic layer was combined, dried over sodium sulfate and concentrated in vacuo. Thereto was added DMF (200 mL), and the mixture was ice-cooled. Then, thereto was added hydrazine monohydrate (14.2 mL), and the mixture was stirred at room temperature for 2 hours. Thereto was added brine, and then the mixture was extracted with ethyl acetate. The organic layer was washed with brine, and then dried over sodium sulfate and concentrated in vacuo. The residue was purified by silica gel chromatography (eluent: hexane/ethyl acetate=1/1 to chlorofon methanol=10/1) to give Compound IV (34.74 g).

Step (iv):

To an ice-cooled mixed solution of Compound IV (34.74 g), sodium bicarbonate (18.9 g) and 95% ethanol (300 mL) was added ethyl bromopyruvate (17.7 mL). The mixture was stirred at room temperature for 20 minutes, and then stirred at 90° C. After 1.5 hours, thereto was added acetic acid (200 mL), and the mixture was stirred at 125° C. while removing solvents with a Dean-Stark apparatus. The mixture was stirred overnight, and then the mixture was cooled back to room temperature and concentrated in vacuo. To the residue was added saturated sodium bicarbonate water, and the mixture was extracted with ethyl acetate. The organic layer was dried over sodium sulfate and concentrated in vacuo. The residue was purified by silica gel chromatography (eluent: hexane/ethyl acetate=1/1 to chloroform/methanol=10/1) to give the titled Compound V (20.9 g) (49.8% yields).

¹H-NMR (CDCl₃) δ 1.39 (t, J=8 Hz, 3H), 2.09 (m, 2H), 2.82 (s, 3H), 3.34-3.48 (m, 2H), 3.56-3.75 (m, 2H), 4.37 (q, J=8 Hz, 2H), 4.45 (m, 1H), 5.15 (s, 2H), 6.20 (s, 1H), 7.31-7.41 (m, 5H), 9.85 (br, 1H)

Reference Example 17
Ethyl 5-[{(3S)-1-[(benzyloxy)carbonyl]pyrrolidin-3-yl}(methyl)amino]-1H-pyrazole-3-carboxylate

The titled compound was prepared in the similar manner to Reference Example 16.

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¹H-NMR (CDCl₃) δ 1.39 (t, J=8 Hz, 3H), 2.09 (m, 2H), 2.82 (s, 3H), 3.33-3.49 (m, 2H), 3.56-3.76 (m, 2H), 4.37 (q, J=8 Hz, 2H), 4.45 (m, 1H), 5.15 (s, 2H), 6.20 (s, 1H), 7.31-7.41 (m, 5H), 9.85 (br, 1H)

Reference Example 18
Benzyl (3R)-3-[[3-(ethoxycarbonyl)-1H-pyrazol-5-yl](methyl)amino]piperidine-1-carboxylate

The titled compound was prepared in the similar manner to Reference Example 16.

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¹H-NMR (CDCl₃) δ 1.35-1.39 (m, 3H), 1.51-1.74 (m, 4H), 1.78-1.81 (m, 1H), 1.92-1.95 (m, 1H), 2.65-2.73 (m, 1H), 2.82 (s, 3H), 3.57-3.64 (m, 1H), 4.12-4.27 (m, 2H), 4.33-4.38 (m, 2H), 5.13 (s, 2H), 6.17 (s, 1H), 7.29-7.39 (m, 5H)

Reference Example 19
Benzyl (3S)-3-[[3-(ethoxycarbonyl)-1H-pyrazol-5-yl](methyl)amino]piperidine-1-carboxylate

The titled compound was prepared in the similar manner to Reference Example 16.

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Example 1
N-[(E)-5-(aminocarbonyl)-2-adamantyl]-5-(dimethylamino)-1-methyl-1H-pyrazole-3-carboxamide and
N-[(Z)-5-(aminocarbonyl)-2-adamantyl]-5-(dimethylamino)-1-methyl-1H-pyrazole-3-carboxamide

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Step (i):

Compound I (153 mg) was dissolved in DMF (5 mL), and then thereto were added methyl 4-aminoadamantane-1-carboxylate hydrochloride (200 mg), WSC.HCl (217 mg), HOBt.H₂O (146 mg) and triethylamine (158 μL), and the mixture was stirred at room temperature for 6 hours. Thereto was added water, and the mixture was extracted with ethyl acetate. The organic layer was dried over sodium sulfate and concentrated in vacuo. The residue was purified by preparative TLC (eluent: chloroform/methanol=10/1) to give Compound II (82 mg).

Step (ii):

Compound II (72 mg) was dissolved in methanol (3 ml), and then thereto was added 2N sodium hydroxide solution (1 mL) and the mixture was stirred at room temperature overnight and concentrated in vacuo. Then, the mixture was acidified by diluted hydrochloric acid, and then extracted with ethyl acetate. The organic layer was dried over sodium sulfate and concentrated in vacuo. The residue was dissolved in DMF (5 ml), and thereto were added ammonium chloride (13 mg), WSCI.HCl (45 mg), HOBt.H₂O (32 mg) and triethylamine (55 μL) and the mixture was stirred at room temperature overnight. Thereto was added water, and then the mixture was extracted with ethyl acetate. The organic layer was dried over sodium sulfate and concentrated in vacuo. The residue was purified by preparative TLC (eluent: chloroform/methanol=10/1) to give Compound III (16 mg) as a high-polar ingredient and Compound IV (4.9 mg) as a low-polar one. The structures were determined by X-ray crystallographic analysis.

N-[(E)-5-(Aminocarbonyl)-2-adamantyl]-5-(dimethylamino)-1-methyl-1H-pyrazole-3-carboxamide

A high-polar ingredient

¹H-NMR (CDCl₃) δ 1.63-1.68 (m, 3H), 1.93-1.97 (m, 4H), 2.00-2.08 (m, 4H), 2.08-2.16 (m, 2H), 2.69 (s, 6H), 3.75 (s, 3H), 4.19-4.21 (m, 1H), 5.27 (s, 1H), 5.62 (s, 1H), 6.31 (s, 1H), 7.17 (m, 1H)

N-[(Z)-5-(Aminocarbonyl)-2-adamantyl]-5-(dimethylamino)-1-methyl-1H-pyrazole-3-carboxamide

A low-polar ingredient

¹H-NMR (CDCl₃) δ 1.55-1.68 (m, 4H), 1.77-1.84 (m, 3H), 1.93-1.97 (m, 3H), 2.08-2.10 (m, 2H), 2.20 (m, 1H), 2.70 (s, 6H), 3.75 (s, 3H), 4.15 (m, 1H), 5.24 (s, 1H), 5.65 (s, 1H), 6.32 (m, 1H), 7.13 (m, 1H)

Example 2
5-[Cyclopropyl(3-methoxybenzyl)amino]-N-[(E)-5-hydroxy-2-adamantyl]-1-methyl-1H-pyrazole-3-carboxamide

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Step (i):

Compound I (24 mg) was dissolved in methanol (5 mL), and then thereto was added 2N sodium hydroxide solution (500 μL) and the mixture was stirred at room temperature overnight. The reaction solution was concentrated in vacuo, and then acidified by 1N hydrochloric acid and extracted with ethyl acetate. The organic layer was dried over sodium sulfate and concentrated in vacuo to give Compound II (14 mg).

Step (ii):

Compound II (14 mg) was dissolved in DMF (2 mL), and then thereto were added (E)-4-aminoadamantan-1-ol hydrochloride (14 mg), WSCI.HCl (86 mg), HOBt.H₂O (60 mg) and triethylamine (19 μL), and the mixture was stirred at room temperature overnight. Thereto was added water, and then the mixture was extracted with ethyl acetate. The organic layer was dried over sodium sulfate and concentrated in vacuo. The residue was purified by preparative TLC (eluent: chloroform/methanol=10/1) to give Compound III (21 mg).

¹H-NMR (CDCl₃) δ 0.44-0.46 (m, 2H), 0.56-0.57 (m, 2H), 1.52-1.55 (m, 2H), 1.64 (m, 1H), 1.78-1.80 (m, 4H), 1.85-1.95 (m, 4H), 2.19-2.22 (m, 3H), 2.44 (m, 1H), 3.55 (s, 3H), 3.75 (s, 3H), 4.10 (s, 2H), 4.17 (m, 1H), 6.67 (s, 1H), 6.76 (m, 1H), 6.80 (m, 1H), 7.10 (m, 1H), 7.19 (m, 1H)

The following compounds were synthesized in the similar manner to Example 2.

Example 3
5-[Cyclopropyl(4-methoxybenzyl)amino]-N-[(E)-5-hydroxy-2-adamantyl]-1-methyl-1H-pyrazole-3-carboxamide

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¹H-NMR (CDCl₃) δ 0.42-0.43 (m, 2H), 0.55-0.57 (m, 2H), 1.52-1.60 (m, 3H), 1.78-1.80 (m, 4H), 1.86-1.96 (m, 4H), 2.19-2.22 (m, 3H), 2.39 (m, 1H), 3.52 (s, 3H), 3.79 (s, 3H), 4.06 (s, 2H), 4.18 (m, 1H), 6.45 (s, 1H), 6.78-6.81 (m, 2H), 7.01-7.05 (m, 2H), 7.11 (m, 1H)

Example 4
N-[(E)-5-Hydroxy-2-adamantyl]-1-methyl-5-[methyl(2-phenoxyethyl)amino]-1H-pyrazole-3-carboxamide

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¹H-NMR (CDCl₃) δ 1.52-1.54 (m, 3H), 1.78-1.80 (m, 4H), 1.85-1.88 (m, 2H), 1.92-1.95 (m, 2H), 2.18-2.22 (m, 3H), 2.78 (s, 3H), 3.28 (t, 5.4 Hz, 2H), 3.75 (s, 3H), 4.04 (t, 5.4 Hz, 2H), 4.16-4.18 (m, 1H), 6.44 (s, 1H), 6.85-6.87 (m, 2H), 6.93-6.97 (m, 1H), 7.09-7.11 (m, 1H), 7.27-7.29 (m, 2H)

Example 5
5-[[4-(4-Fluorophenoxy)benzyl](methyl)amino]-N-[(E)-5-hydroxy-2-adamantyl]-1-methyl-1H-pyrazole-3-carboxamide

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¹H-NMR (CDCl₃) δ 1.52-1.55 (m, 3H), 1.78-1.80 (m, 4H), 1.84-1.88 (m, 2H), 1.92-1.95 (m, 2H), 2.19-2.21 (m, 3H), 2.61 (s, 3H), 3.74 (s, 3H), 3.96 (s, 2H), 4.16-4.18 (m, 1H), 6.40 (s, 1H), 6.90-6.92 (m, 2H), 6.96-7.06 (m, 4H), 7.09-7.11 (m, 1H), 7.22-7.24 (m, 2H)

Example 6
N-[(E)-5-Hydroxy-2-adamantyl]-1-methyl-5-{methyl[3-(methylsulfonyl)benzyl]amino}-1H-pyrazole-3-carboxamide

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¹H-NMR (CDCl₃) δ 1.50-1.58 (m, 2H), 1.75-1.94 (m, 9H), 2.20 (m, 3H), 2.64 (s, 3H), 3.06 (s, 3H), 3.73 (s, 3H), 4.12 (s, 2H), 4.13-4.19 (m, 1H), 6.39 (s, 1H), 7.08-7.15 (m, 1H), 7.51-7.63 (m, 2H), 7.74-7.90 (m, 2H)

Example 7
N-[(E)-5-Carbamoyladamantan-2-yl]-1-methyl-5-[methyl(propyl)amino]-1H-pyrazole-3-carboxamide

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¹H-NMR (CDCl₃) δ 0.82 (t, J=7.1 Hz, 3H), 1.39-1.47 (m, 2H), 1.54-1.57 (m, 2H), 1.70 (m, 1H), 1.85-1.89 (m, 4H), 1.93-1.97 (m, 4H), 1.99-2.10 (m, 2H), 2.57 (s, 3H), 2.71-2.78 (m, 2H), 3.66 (s, 3H), 4.12 (m, 1H), 5.37 (bs, 1H), 5.60 (bs, 1H), 6.28 (s, 1H), 7.10 (m, 1H)

Example 8

N-[(E)-5-Carbamoyladamantan-2-yl]-5-[isopropyl(methyl)amino]-1-methyl-1H-pyrazole-3-carboxamide

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¹H-NMR (CDCl₃) δ 1.10 (d, J=6.5 Hz, 6H), 1.54 (m, 1H), 1.63-1.66 (m, 2H), 1.94-1.99 (m, 4H), 2.02-2.08 (m, 4H), 2.18-2.20 (m, 2H), 2.58 (s, 3H), 3.17 (sept, J=6.5 Hz, 1H), 3.73 (s, 3H), 4.21 (m, 1H), 5.20 (bs, 1H), 5.59 (bs, 1H), 6.42 (s, 1H), 7.01 (m, 1H)

Example 9
N-[(E)-5-Carbamoyladamantan-2-yl]-5-[ethyl(methyl)amino]-1-methyl-1H-pyrazole-3-carboxamide

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¹H-NMR (CDCl₃) δ 1.09 (t, J=7.2 Hz, 3H), 1.62-1.67 (m, 3H), 1.93-1.98 (m, 4H), 2.02-2.08 (m, 4H), 2.18-2.20 (m, 2H), 2.65 (s, 3H), 2.92 (q, J=7.2 Hz, 2H), 3.73 (s, 3H), 4.20 (m, 1H), 5.33 (bs, 1H), 5.63 (bs, 1H), 6.36 (s, 1H), 7.16 (m, 1H)

Example 10
N-[(E)-5-Carbamoyladamantan-2-yl]-5-[isobutyl(methyl)amino]-1-methyl-1H-pyrazole-3-carboxamide

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¹H-NMR (CDCl₃) δ 0.90 (m, 6H), 1.57-1.68 (m, 2H), 1.78 (m, 1H), 1.90-2.09 (m, 9H), 2.13-2.21 (m, 2H), 2.60-2.64 (m, 5H), 3.73 (s, 3H), 4.17-4.23 (m, 1H), 5.32 (bs, 1H), 5.63 (bs, 1H), 6.35 (s, 1H), 7.14-7.20 (m, 1H)

Example 11
N-[(E)-5-Carbamoyladamantan-2-yl]-4-chloro-5-(dimethylamino)-1-methyl-1H-pyrazole-3-carboxamide

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¹H-NMR (CDCl₃) δ 1.53-1.56 (m, 2H), 1.63-1.66 (m, 2H), 1.90-1.94 (m, 3H), 2.03-2.07 (m, 4H), 2.18-2.22 (m, 2H), 2.86 (s, 6H), 3.73 (s, 3H), 4.22 (m, 1H), 5.19 (bs, 1H), 5.59 (bs, 1H), 7.08 (m, 1H)

Example 12
N-[(E)-5-Hydroxyadamantan-2-yl]-1-methyl-5-{methyl[4-(methylsulfonyl)benzyl]amino}-1H-pyrazole-3-carboxamide

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¹H-NMR (CDCl₃) δ 1.47-1.58 (m, 2H), 1.73-1.96 (m, 9H), 2.13-2.25 (m, 3H), 2.65 (s, 3H), 3.05 (s, 3H), 3.75 (s, 3H), 4.11 (s, 2H), 4.13-4.18 (m, 1H), 6.41 (s, 1H), 7.08-7.13 (m, 1H), 7.49-7.53 (m, 2H), 7.88-7.91 (m, 2H)

Example 13
N-[(E)-5-Carbamoyladamantan-2-yl]-5-[(cyclopropylmethyl)(propyl)amino]-1-methyl-1H-pyrazole-3-carboxamide

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¹H-NMR (CDCl₃) δ 0.02-0.05 (m, 2H), 0.40-0.45 (m, 2H), 0.81-0.88 (m, 1H), 0.86 (t, J=7.4 Hz, 3H), 1.36-1.43 (m, 2H), 1.60-1.65 (m, 2H), 1.93-2.18 (m, 11H), 2.73 (d, J=6.8 Hz, 2H), 2.91 (t, J=7.4 Hz, 2H), 3.76 (s, 3H), 4.19-4.21 (m, 1H), 5.16 (bs, 1H), 5.58 (bs, 1H), 6.47 (s, 1H), 7.19-7.21 (m, 1H)

Example 14
N-[(E)-5-Carbamoyladamantan-2-yl]-5-[(2-methoxyethyl)(methyl)amino]-1-methyl-1H-pyrazole-3-carboxamide

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¹H-NMR (CDCl₃) δ 1.58-1.66 (m, 2H), 1.90-2.09 (m, 9H), 2.13-2.20 (m, 2H), 2.72 (s, 3H), 3.05 (m, 2H), 3.34 (s, 3H), 3.46 (m, 2H), 3.75 (s, 3H), 4.16-4.23 (m, 1H), 5.29 (bs, 1H), 5.62 (bs, 1H), 6.38 (s, 1H), 7.14-7.20 (m, 1H)

Example 15
N-[(E)-5-Carbamoyladamantan-2-yl]-5-[(cyclopropylmethyl)(methyl)amino]-1-methyl-1H-pyrazole-3-carboxamide

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¹H-NMR (CDCl₃) δ 0.08-0.11 (m, 2H), 0.43-0.53 (m, 2H), 0.85-0.95 (m, 1H), 1.58-1.68 (m, 2H), 1.90-2.09 (m, 9H), 2.13-2.20 (m, 2H), 2.68-2.74 (m, 5H), 3.74 (s, 3H), 4.15-4.23 (m, 1H), 5.25 (bs, 1H), 5.60 (bs, 1H), 6.37 (s, 1H), 7.14-7.20 (m, 1H)

Example 16
5-(Cyclopropyl{[1-(3,3,3-trifluoropropyl)piperidin-4-yl]methyl}amino)-N-[(E)-5-hydroxyadamantan-2-yl]-1-methyl-1H-pyrazole-3-carboxamide

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Step (i):

Compound I (50 mg) was dissolved in dichloromethane (1 mL), and then thereto were added 3,3,3-trifluoro propionaldehyde (23 mg) and acetic acid (100 μL). The mixture was stirred at room temperature for 1.5 hours, and then thereto was added NaBH(OAc)₃(60 mg) and the mixture was stirred at room temperature overnight. Thereto was added water, and the mixture was extracted with chloroform. The organic layer was washed with saturated sodium bicarbonate water. The organic layer was dried over sodium sulfate and concentrated in vacuo. The residue was purified by preparative TLC (eluent: chloroform/methanol=10/1) to give the titled Compound II (48 mg).

¹H-NMR (CDCl₃) δ 0.35-0.44 (m, 2H), 0.53-0.61 (m, 2H), 1.13-1.28 (m, 2H), 1.46-1.98 (m, 16H), 2.16-2.35 (m, 5H), 2.45 (m, 1H), 2.52-2.59 (m, 2H), 2.81-2.92 (m, 4H), 3.68 (s, 3H), 4.13-4.20 (m, 1H), 6.43 (s, 1H), 7.09-7.14 (m, 1H)

Example 17
5-(Cyclopropyl{[1-(2-methoxyethyl)piperidin-4-yl]methyl}amino)-N-[(E)-5-hydroxyadamantan-2-yl]-1-methyl-1H-pyrazole-3-carboxamide

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¹H-NMR (CDCl₃) δ 0.36-0.45 (m, 2H), 0.53-0.61 (m, 2H), 1.20-1.33 (m, 2H), 1.46-1.72 (m, 6H), 1.74-1.98 (m, 10H), 2.15-2.26 (m, 3H), 2.45 (m, 1H), 2.50-2.58 (m, 2H), 2.85-2.97 (m, 4H), 3.34 (s, 3H), 3.49 (m, 2H), 3.67 (s, 3H), 4.14-4.20 (m, 1H), 6.43 (s, 1H), 7.09-7.14 (m, 1H)

Example 18
N-[(E)-5-Carbamoyladamantan-2-yl]-5-(diethylamino)-1-methyl-1H-pyrazole-3-carboxamide

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¹H-NMR (CDCl₃) δ 1.00 (t, J=7.1 Hz, 6H), 1.62-1.65 (m, 2H), 1.93-2.18 (m, 11H), 2.93 (q, J=7.1 Hz, 4H), 3.73 (s, 3H), 4.19-4.21 (m, 1H), 5.17 (bs, 1H), 5.58 (bs, 1H), 6.44 (s, 1H), 7.19-7.21 (m, 1H)

Example 19
N-[(E)-5-Carbamoyladamantan-2-yl]-5-[cyclobutyl(methyl)amino]-1-methyl-1H-pyrazole-3-carboxamide

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¹H-NMR (CDCl₃) δ 1.40-1.70 (m, 4H), 1.77-1.87 (m, 2H), 1.93-2.11 (m, 11H), 2.17-2.18 (m, 2H), 2.52 (s, 3H), 3.48-3.56 (m, 1H), 3.73 (s, 3H), 4.19-4.21 (m, 1H), 5.19 (bs, 1H), 5.58 (bs, 1H), 6.33 (s, 1H), 7.17-7.19 (m, 1H)

Example 20
5-[Cyclopropyl(piperidin-4-ylmethyl)amino]-N-[(E)-5-hydroxyadamantan-2-yl]-1-methyl-1H-pyrazole-3-carboxamide

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¹H-NMR (CDCl₃) δ 0.40-0.45 (m, 2H), 0.55-0.61 (m, 2H), 1.50-1.67 (m, 4H), 1.68-1.97 (m, 13H), 2.15-2.26 (m, 3H), 2.48 (m, 1H), 2.72-2.83 (m, 2H), 2.94-2.99 (m, 2H), 3.41-3.46 (m, 2H), 3.69 (s, 3H), 4.13-4.19 (m, 1H), 6.43 (s, 1H), 7.09-7.15 (m, 1H)

Example 21
5-{[(1-Acetylpiperidin-4-yl)methyl](cyclopropyl)amino}-N-[(E)-5-hydroxyadamantan-2-yl]-1-methyl-1H-pyrazole-3-carboxamide

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¹H-NMR (CDCl₃) δ 0.38-0.45 (m, 2H), 0.54-0.61 (m, 2H), 1.01-1.15 (m, 2H), 1.41-1.65 (m, 5H), 1.67-1.82 (m, 5H), 1.83-1.97 (m, 4H), 2.06 (s, 3H), 2.15-2.28 (m, 3H), 2.42-2.53 (m, 2H), 2.86-2.31 (m, 3H), 3.69 (s, 3H), 3.73-3.81 (m, 1H), 4.13-4.21 (m, 1H), 4.53-4.62 (m, 1H), 6.44 (s, 1H), 7.09-7.15 (m, 1H)

Example 22
N-[(E)-5-(Aminocarbonyl)-2-adamantyl]-4-chloro-5-[[1-(4-methoxyphenyl)cyclopropyl](methyl)amino]-1-methyl-1H-pyrazole-3-carboxamide

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Step (i):

1-(4-Methoxyphenyl)-cyclopropanecarboxylic acid (5.0 g) was dissolved in toluene (80 mL), and then thereto were added triethylamine (3.8 mL) and diphenyl phosphoryl azide (5.9 mL), and the mixture was stirred at 100° C. for 5 hours. The reaction solvent was concentrated in vacuo, and then dissolved in THF (80 ml). Then, thereto was added 2N sodium hydroxide solution (30 mL), and the mixture was stirred at room temperature for 2 hours. The mixture was concentrated in vacuo, and then thereto was added 1N hydrochloric acid and the mixture was extracted with ethyl acetate. The aqueous layer was basified by sodium hydroxide solution and extracted with chloroform. The organic layer was dried over sodium sulfate and concentrated in vacuo to give Compound II (2.9 g).

Step (ii):

Compound II (2.9 g) was dissolved in ethyl formate (30 mL) and stirred in a sealed tube at 90° C. for 3 days, and the reaction solvent was concentrated in vacuo. The residue was dissolved in THF (10 mL) and added dropwise to a solution of lithium aluminum hydride (2.7 g) in THF (80 mL). The mixture was stirred at 80° C. for 3 hours, and then thereto were added water (3 mL), 15% sodium hydroxide solution (3 mL) and water (9 mL) at 0° C. in sequence. The reaction solution was filtered through Celite®. The filtrate was concentrated in vacuo to give Compound III (2.5 g).

Step (iii):

4-Chlorophenyl chlorothioformate (2.9 g) was dissolved in THF (30 mL), and thereto were added triethylamine (2.1 mL) and Compound III (2.5 g) at 0° C. and the mixture was stirred at room temperature for 2 hours. The reaction solution was concentrated in vacuo, and then thereto was added water and the mixture was extracted with ethyl acetate. The organic layer was washed with brine, dried over sodium sulfate, and then concentrated in vacuo. The residue was dissolved in NMP (30 mL), and then thereto was added hydrazine monohydrate (3.4 mL) and the mixture was stirred at 70° C. for 6 hours. Thereto was added water, and the mixture was extracted with ethyl acetate. The organic layer was washed with brine, and then dried over sodium sulfate and concentrated in vacuo. The residue was purified by silica gel chromatography (eluent: hexane/ethyl acetate=7/3) to give Compound IV (2.1 g).

Step (iv):

Compound IV (2.1 g) was dissolved in a mixed solvent of ethanol (25 mL) and THF (25 mL), and thereto were added sodium bicarbonate (690 mg) and ethyl bromopyruvate (1.2 mL) and the mixture was stirred at 70° C. for 4 hours. The reaction solution was concentrated in vacuo, and then thereto was added acetic acid (50 mL) and the mixture was stirred at 80° C. for 5 hours and concentrated in vacuo. Then, thereto was added saturated sodium bicarbonate water, and the mixture was extracted with ethyl acetate. The organic layer was dried over sodium sulfate and concentrated in vacuo. The residue was purified by silica gel chromatography (eluent: hexane/ethyl acetate=2/1) to give Compound V (1.6 g).

Step (v):

To a solution of sodium hydride (250 mg) in THF (20 mL) was added dropwise a solution of Compound V (1.6 g) in THF (5 mL) at 0° C., and the mixture was stirred at room temperature for 1 hour. Then, thereto was slowly added methyl iodide (380 μL) at 0° C., and the mixture was stirred at room temperature overnight. Then, thereto was added water, and then the mixture was concentrated in vacuo. Thereto was added saturated sodium bicarbonate water, and the mixture was extracted with chloroform. The organic layer was dried over sodium sulfate and concentrated in vacuo. The residue was purified by silica gel chromatography (eluent: hexane/ethyl acetate=2/1) to give Compound VI (1.2 g).

Step (vi):

Compound VI (599 mg) was dissolved in DMF (10 mL), and then thereto was added N-chlorosuccinimide (267 mg) in small portions, and the mixture was stirred at room temperature overnight. Then, thereto was added water, and then the mixture was extracted with ethyl acetate. The organic layer was sequentially washed with saturated sodium bicarbonate water and brine. The organic layer was dried over sodium sulfate and concentrated in vacuo. The residue was purified by silica gel chromatography (eluent: hexane/ethyl acetate=3/1) to give Compound VII (530 mg).

Step (vii):

Compound VII (530 mg) was dissolved in ethanol (15 mL), and then thereto was added 2N lithium hydroxide solution (2.2 mL), and the mixture was stirred at room temperature overnight. The reaction solution was concentrated in vacuo, and then acidified by 1N hydrochloric acid and extracted with chloroform. The organic layer was dried over sodium sulfate and concentrated in vacuo to give Compound VIII (490 mg).

Step (viii):

Compound VIII (80 mg) was dissolved in DMF (1.5 mL), and then thereto were added methyl (E)-4-aminoadamantane-1-carboxylate (50 mg), WSCI.HCl (69 mg), HOBt.H₂O (49 mg) and triethylamine (100 μL), and the mixture was stirred at room temperature overnight. Then, thereto was added water, and then the mixture was extracted with ethyl acetate. The organic layer was sequentially washed with saturated sodium bicarbonate water, 1N hydrochloric acid and brine. The organic layer was dried over sodium sulfate and concentrated in vacuo. The residue was dissolved in methanol (1.5 mL), and then thereto was added 2N lithium hydroxide solution (400 μL) and the mixture was stirred at room temperature overnight. The reaction solution was concentrated in vacuo, and then acidified by 1N hydrochloric acid and extracted with ethyl acetate. The organic layer was dried over sodium sulfate and concentrated in vacuo. The residue was dissolved in DMF (1.5 mL), and then thereto were added ammonium chloride (134 mg), WSCI.HCl (69 mg), HOBt.H₂O (49 mg) and triethylamine (460 μL) and the mixture was stirred at room temperature overnight. Then, thereto was added water, and then the mixture was extracted with ethyl acetate. The organic layer was sequentially washed with saturated sodium bicarbonate water, 1N hydrochloric acid and brine. The organic layer was dried over sodium sulfate and concentrated in vacuo. The residue was purified by preparative TLC (eluent: chlorofol methanol=10/1) to give the titled Compound IX (101 mg) as a white solid.

¹H-NMR (CDCl₃) δ 0.98-1.01 (m, 2H), 1.10-1.13 (m, 2H), 1.63-1.66 (m, 2H), 1.89-2.19 (m, 11H), 2.96 (s, 3H), 3.61 (s, 3H), 3.81 (s, 3H), 4.23-4.25 (m, 1H), 6.11 (bs, 1H), 6.64 (bs, 1H), 6.85-6.90 (m, 2H), 7.13-7.15 (m, 1H), 7.22-7.25 (m, 2H)

The following compounds were obtained in the similar manner.

[Chemical Formula 68]

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Example No.
R_D
A

23
H
CONH₂

24
Cl
OH

25
H
OH

Example 23

¹H-NMR (CDCl₃) δ 1.03-1.11 (m, 4H), 1.61-2.18 (m, 13H), 2.82 (s, 3H), 3.71 (s, 3H), 3.81 (s, 3H), 4.19-4.21 (m, 1H), 5.24 (bs, 1H), 5.61 (bs, 1H), 6.24 (s, 1H), 6.87-6.91 (m, 2H), 7.17-7.20 (m, 2H), 7.45-7.47 (m, 1H)

Example 24

¹H-NMR (CDCl₃) δ 0.98-1.01 (m, 2H), 1.11-1.14 (m, 2H), 1.52-1.55 (m, 2H), 1.77-1.85 (m, 7H), 1.91-1.94 (m, 2H), 2.18-2.22 (m, 3H), 2.97 (s, 3H), 3.59 (s, 3H), 3.81 (s, 3H), 4.18-4.20 (m, 1H), 6.87-6.90 (m, 2H), 7.05-7.06 (m, 1H), 7.23-7.25 (m, 2H)

Example 25

¹H-NMR (CDCl₃) δ 0.97-1.04 (m, 4H), 1.61-1.64 (m, 3H), 1.69-1.81 (m, 6H), 1.93-1.96 (m, 2H), 2.15-2.16 (m, 1H), 2.27 (bs, 2H), 2.75 (s, 3H), 3.65 (s, 3H), 3.81 (s, 3H), 4.08-4.10 (m, 1H), 6.21 (s, 1H), 6.87-6.91 (m, 2H), 7.18-7.25 (m, 3H)

Example 26

N-[(E)-5-(Aminocarbonyl)-2-adamantyl]-4-fluoro-5-[isopropylmethyl)amino]-1-methyl-1H-pyrazole-3-carboxamide

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Step (i):

To a solution of Compound I (200 mg) in DMF (15 mL) was added saturated sodium bicarbonate water (3 mL), and then thereto was added 1-chloromethyl-4-fluoro-1,4-diazoniabicyclo[2.2.2.]octane bis(tetrafluoroborate) (638 mg) in small portions and the mixture was stirred at room temperature for 3 hours. Then, thereto was added water, and then the mixture was extracted with ethyl acetate. The organic layer was dried over sodium sulfate and concentrated in vacuo. The residue was purified by silica gel chromatography (eluent: hexane/ethyl acetate=3/1) to give Compound II (64 mg).

Step (ii):

Compound II (65 mg) was dissolved in ethanol (1.5 mL), and then thereto was added 2N lithium hydroxide solution (380 μL) and the mixture was stirred at room temperature overnight. The reaction solution was concentrated in vacuo, and then acidified by 1N hydrochloric acid and extracted with ethyl acetate. The organic layer was dried over sodium sulfate and concentrated in vacuo to give Compound III (45 mg).

Step (iii):

Compound III (23 mg) was dissolved in DMF (0.5 mL), and then thereto were added methyl (E)-4-aminoadamantane-1-carboxylate (31 mg), WSCI.HCl (29 mg), HOBt.H₂O (20 mg) and triethylamine (40 μL), and the mixture was stirred at room temperature overnight. Then, thereto was added water, and then the mixture was extracted with ethyl acetate. The organic layer was sequentially washed with saturated sodium bicarbonate water, 1N hydrochloric acid and brine. The organic layer was dried over sodium sulfate and concentrated in vacuo. The residue was dissolved in methanol (0.5 mL), and then thereto was added 2N lithium hydroxide solution (140 μL) and the mixture was stirred at room temperature overnight. The reaction solution was concentrated in vacuo, and then acidified by 1N hydrochloric acid and extracted with ethyl acetate. The organic layer was dried over sodium sulfate and concentrated in vacuo. The residue was dissolved in DMF (0.5 mL), and then thereto were added ammonium chloride (53 mg), WSCI.HCl (28 mg), HOBt.H₂O (20 mg) and triethylamine (130 μL) and the mixture was stirred at room temperature overnight. Then, thereto was added water, and then the mixture was extracted with ethyl acetate. The organic layer was sequentially washed with saturated sodium bicarbonate water, 1N hydrochloric acid and brine. The organic layer was dried over sodium sulfate and concentrated in vacuo. The residue was purified by preparative TLC (eluent: chloroform/methanol=10/1) to give the titled Compound IV (22 mg) as a white solid.

¹H-NMR (CDCl₃) δ 1.10-1.12 (m, 6H), 1.63-1.66 (m, 2H), 1.89-2.18 (m, 11H), 2.74 (s, 3H), 3.18-3.27 (m, 1H), 3.71 (s, 3H), 4.20-4.25 (m, 1H), 6.00 (bs, 1H), 6.41 (bs, 1H), 6.93-6.95 (m, 1H)

Example 27
5-[[(1-Acetylpiperidin-4-yl)methyl] (methyl)amino]-N-[(E)-5-hydroxy-2-adamantyl]-1-methyl-1H-pyrazole-3-carboxamide

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Step (i):

Compound I (6.2 g) was dissolved in dichloromethane (70 mL), and then thereto were added 2M methylamine-THF solution (40 mL) and acetic acid (2 mL), and the mixture was stirred at room temperature for 1 hour. Then, thereto was added NaBH(OAc)₃(8.0 g) and the mixture was stirred at room temperature overnight. Then, thereto was added water, and then the mixture was concentrated in vacuo. Then, thereto was added saturated sodium bicarbonate water, and the mixture was extracted with chloroform. The organic layer was dried over sodium sulfate and concentrated in vacuo. The residue was purified by silica gel chromatography (eluent: chloroform/meththanol=10/1) to give Compound II (2.3 g).

Step (ii):

To a solution of 1,1′-thiocarbonyldiimidazole (1.1 g) in THF (25 mL) was added a solution of Compound II (2.3 g) in THF (5 mL), and the mixture was stirred at room temperature overnight. Then, thereto was added hydrazine monohydrate (1.7 mL), and the mixture was stirred at 70° C. for 6 hours and concentrated in vacuo. Then, thereto was added brine, and the mixture was extracted with chloroform. The organic layer was dried over sodium sulfate, and then concentrated in vacuo. The residue was purified by silica gel chromatography (eluent: chloroform/methanol=5/1) to give Compound III (2.5 g).

Step (iii):

Compound III (2.5 g) was dissolved in a mixed solvent of ethanol (25 mL) and THF (25 mL), and then thereto were added sodium bicarbonate (680 mg) and ethyl bromopyruvate (1.1 mL) and the mixture was stirred at 80° C. for 4 hours. Then, thereto was added acetic acid (25 mL), and the mixture was stirred at 70° C. for 3 hours and concentrated in vacuo. Then, thereto was added saturated sodium bicarbonate water, and the mixture was extracted with ethyl acetate. The organic layer was dried over sodium sulfate and concentrated in vacuo. The residue was purified by silica gel chromatography (eluent: hexane/ethyl acetate=1/1) to give Compound IV (1.3 g).

Step (iv):

To a solution of sodium hydride (160 mg) in THF (10 mL) was added dropwise a solution of Compound IV (1.3 g) in THF (6 mL) at 0° C., and the mixture was stirred at room temperature for 1 hour. Then, thereto was slowly added methyl iodide (310 μL) at 0° C., and the mixture was stirred at room temperature overnight. Then, thereto was added water, and the mixture was extracted with ethyl acetate. The organic layer was dried over sodium sulfate and concentrated in vacuo. The residue was purified by silica gel chromatography (eluent: hexane/ethyl acetate=2/1) to give Compound V (851 mg).

Step (v):

Compound V (845 mg) was dissolved in ethanol (7 mL), and then thereto was added 6N lithium hydroxide solution (1 mL) and the mixture was stirred at 40° C. for 3 hours. The reaction solution was concentrated in vacuo, and then acidified by 1N hydrochloric acid and extracted with chloroform. The organic layer was dried over sodium sulfate and concentrated in vacuo to give Compound VI (750 mg).

Step (vi):

Compound VI (390 mg) was dissolved in DMF (5 mL), and then thereto were added (E)-4-aminoadamantan-1-ol hydrochloride (305 mg), WSCI.HCl (382 mg), HOBt.H₂O (270 mg) and triethylamine (340 μL), and the mixture was stirred at room temperature overnight. Then, thereto was added saturated sodium bicarbonate water, and then the mixture was extracted with ethyl acetate and the organic layer was washed with brine. The organic layer was dried over sodium sulfate and concentrated in vacuo. The residue was purified by preparative TLC (eluent: chloroform/methanol=10/1) to give Compound VII (450 mg).

Step (vii):

Compound VII (450 mg) was dissolved in methanol (4 mL), and then thereto was added palladium-carbon (50 mg) and the mixture was stirred under hydrogen atmosphere (3 atm) for 4.5 hours. The resulting solid was filtered through Celite®, and then the filtrate was concentrated to give Compound VIII (340 mg).

Step (viii):

Compound VIII (80 mg) was dissolved in dichloromethane (1 mL), and then thereto were added triethylamine (86 μL) and acetyl chloride (30 μL) and the mixture was stirred at room temperature for 3 hours. Then, thereto was added 2N sodium hydroxide solution, and the mixture was extracted with chloroform. The organic layer was dried over sodium sulfate and concentrated in vacuo. The residue was purified by preparative TLC (eluent: chloroform/meththanol=10/1) to give the titled Compound IX (38 mg).

¹H-NMR (CDCl₃) δ 1.05-1.18 (m, 2H), 1.45-1.96 (m, 14H), 2.07 (s, 3H), 2.16-2.21 (m, 3H), 2.42-2.52 (m, 1H), 2.63 (s, 3H), 2.68-2.78 (m, 2H), 2.92-3.05 (m, 1H), 3.72 (s, 3H), 3.73-3.83 (m, 1H), 4.10-4.19 (m, 1H), 4.55-4.65 (m, 1H), 6.38 (s, 1H), 7.08-7.14 (m, 1H)

Example 28
5-[[(1-Acetylpiperidin-4-yl)methyl] (methyl)amino]-4-chloro-N-[(2s,5r)-5-hydroxy-2-adamantyl]-1-methyl-1H-pyrazole-3-carboxamide

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Step (ix):

Compound (16 mg) of Example 27 was dissolved in DMF (200 μL), and then thereto was added N-chlorosuccinimide (6 mg) and the mixture was stirred at 65° C. for 3 hours. Then, thereto was added water, and then the mixture was extracted with ethyl acetate. The organic layer was washed with brine, dried over sodium sulfate and concentrated in vacuo. The residue was purified by preparative TLC (eluent: chloroform/methanol=10/1) to give the titled Compound X (7 mg) as a white solid.

¹H-NMR (CDCl₃) δ 1.05-1.18 (m, 2H), 1.44-1.60 (m, 3H), 1.72-1.97 (m, 9H), 2.08 (s, 3H), 2.15-2.26 (m, 3H), 2.45-2.56 (m, 1H), 2.72-2.85 (m, 5H), 2.91-3.12 (m, 3H), 3.73 (s, 3H), 3.75-3.85 (m, 1H), 4.15-4.25 (m, 1H), 4.55-4.68 (m, 1H), 6.96-7.08 (m, 1H),

Example 29
4-Chloro-5-[cyclobutyl(2,2,2-trifluoroethyl)amino]-N-[(E)-5-hydroxy-2-adamantyl]-1-methyl-1H-pyrazole-3-carboxamide

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Compound (100 mg) of Reference Example 7 was dissolved in DMF (1.5 mL), and then thereto were added (E)-4-aminoadamantan-1-ol hydrochloride (78 mg), WSCI.HCl (122 mg), HOBt.H₂O (86 mg) and triethylamine (150 μL), and the mixture was stirred at room temperature overnight. Then, thereto was added water, and then the mixture was extracted with ethyl acetate. The organic layer was washed with brine. The organic layer was dried over sodium sulfate and concentrated in vacuo. The residue was purified by preparative TLC (eluent: chloroform/methanol=10/1) to give the titled Compound (128 mg) as a white solid.

¹H-NMR (CDCl₃) δ 1.42-1.68 (m, 5H), 1.71-2.00 (m, 10H), 2.02-2.29 (m, 5H), 3.45-3.80 (m, 5H), 3.80-3.95 (m, 1H), 4.16-4.25 (m, 1H), 6.99-7.09 (m, 1H)

Compounds of Examples 30-131 were prepared in the similar manner.

[Chemical Formula 73]

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Example No.
R_A
R_B
R_C
R_D

30
CH₃
CH₂CH₃
CH₃
Cl

31
CH₃

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CH₃
Cl

32
CH₃

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CH₃
Cl

33
CH₃

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CH₂CH₃
H

34
CH₃

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CH₂CH₃
Cl

35
CH₃
CH₃
CH₂CH₃
Cl

36
CH₂CH₃

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CH₃
Cl

37
CH₂CH₃
CH₂CH₃
CH₃
Cl

38
CH₂CH₃

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CH₃
H

39
CH₃

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CH₃
Cl

40
CH₃

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CH₃
Cl

41
CH₃
CH₂—CF₃
CH₃
Cl

42
CH₃
CH₂—CF₃
CH₃
H

43
CH₃

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CH₃
H

Example 30

¹H-NMR (CDCl₃) δ 1.02 (t, J=8.0 Hz, 3H), 1.61-1.63 (m, 4H), 1.88-1.90 (m, 2H), 1.97-2.04 (m, 4H), 2.12-2.16 (m, 2H), 2.30 (m, 1H), 2.80 (s, 3H), 3.10 (q, J=8.0 Hz, 2H), 3.70 (s, 3H), 4.18 (m, 1H), 5.21 (bs, 1H), 5.59 (bs, 1H), 7.08 (m, 1H)

Example 31

¹H-NMR (CDCl₃) δ 1.08 (d, J=4.0 Hz, 6H), 1.86-1.88 (m, 4H), 1.97-2.04 (m, 6H), 2.15-2.17 (m, 3H), 2.77 (s, 3H), 3.38 (m, 1H), 3.70 (s, 3H), 4.20 (m, 1H), 5.25 (bs, 1H), 5.60 (bs, 1H), 7.10 (m, 1H)

Example 32

¹H-NMR (CDCl₃) δ 0.89 (t, J=8.0 Hz, 3H), 1.39 (q, J=8.0 Hz, 2H), 1.61-1.65 (m, 2H), 1.89-1.92 (m, 3H), 1.97-2.06 (m, 4H), 2.15-2.17 (m, 2H), 2.80 (s, 3H), 3.06 (t, J=8.0 Hz, 3H), 3.72 (s, 3H), 4.02 (m, 1H), 4.22 (m, 1H), 5.34 (bs, 1H), 5.66 (bs, 1H), 7.08 (m, 1H)

Example 33

¹H-NMR (CDCl₃) δ 1.10 (d, J=8.0 Hz, 6H), 1.40-1.47 (m, 3H), 1.60-1.67 (m, 2H), 1.88-1.95 (m, 2H), 1.98-2.12 (m, 6H), 2.17-2.23 (m, 2H), 2.60 (s, 3H), 3.13-3.18 (m, 2H), 4.07-4.14 (m, 2H), 4.21 (m, 1H), 5.35 (bs, 1H), 5.64 (bs, 1H), 6.45 (s, 1H), 7.34 (m, 1H)

Example 34

¹H-NMR (CDCl₃) δ 1.10 (d, J=8.0 Hz, 6H), 1.38-1.43 (m, 3H), 1.62-1.69 (m, 2H), 1.89-1.96 (m, 4H), 2.00-2.13 (m, 5H), 2.16-2.23 (m, 2H), 2.79 (s, 3H), 3.39 (m, 1H), 4.11-4.15 (m, 2H), 4.24 (m, 1H), 6.01 (bs, 1H), 6.50 (bs, 1H), 7.18 (m, 1H)

Example 35

¹H-NMR (CDCl₃) δ 1.40 (t, J=8.0 Hz, 3H), 1.59-1.68 (m, 2H), 1.89-1.93 (m, 4H), 1.99-2.06 (m, 5H), 2.19-2.23 (m, 2H), 2.84 (s, 6H), 4.09 (q, J=8.0 Hz, 2H), 4.20 (m, 1H), 5.21 (bs, 1H), 5.60 (bs, 1H), 7.10 (m, 1H)

Example 36

¹H-NMR (CDCl₃) δ 0.90-0.93 (m, 3H), 1.08-1.09 (m, 6H), 1.62-1.65 (m, 2H), 1.92-1.94 (m, 4H), 1.99-2.07 (m, 5H), 2.19 (bs, 2H), 3.17-3.19 (m, 2H), 3.39-3.45 (m, 1H), 3.72 (s, 3H), 4.21-4.23 (m, 1H), 5.44 (bs, 1H), 5.65 (bs, 1H), 7.12-7.14 (m, 1H)

Example 37

¹H-NMR (CDCl₃) δ 0.98-1.01 (m, 6H), 1.60-1.66 (m, 2H), 1.91-1.93 (m, 4H), 1.99-2.09 (m, 5H), 2.19 (bs, 2H), 3.13-3.19 (m, 4H), 3.74 (s, 3H), 4.21-4.23 (m, 1H), 5.34 (bs, 1H), 5.70 (bs, 1H), 7.11-7.13 (m, 1H)

Example 38

¹H-NMR (CDCl₃) δ 0.88-0.92 (m, 4H), 1.04-1.05 (m, 6H), 1.61-1.65 (m, 2H), 1.93-2.08 (m, 9H), 2.18 (bs, 1H), 2.88-2.94 (m, 2H), 3.11-3.18 (m, 1H), 3.73 (s, 3H), 4.20-4.22 (m, 1H), 5.33 (bs, 1H), 5.61 (bs, 1H), 6.49 (s, 1H), 7.21-7.23 (m, 1H)

Example 39

¹H-NMR (CDCl₃) δ 1.59-1.66 (m, 2H), 1.85-2.09 (m, 9H), 2.15-2.24 (m, 2H), 2.88 (s, 3H), 3.26-3.33 (m, 5H), 3.35-3.41 (m, 2H), 3.75 (s, 3H), 4.15-4.24 (m, 1H), 5.62 (bs, 1H), 5.78 (bs, 1H), 7.06-7.14 (m, 1H)

Example 40

¹H-NMR (CDCl₃) δ 0.89-0.93 (m, 6H), 1.59-1.78 (m, 3H), 1.85-2.09 (m, 9H), 2.15-2.21 (m, 2H), 2.77 (s, 3H), 2.88-2.93 (m, 2H), 3.74 (s, 3H), 4.17-4.25 (m, 1H), 5.45 (bs, 1H), 5.71 (bs, 1H), 7.07-7.14 (m, 1H)

Example 41

¹H-NMR (CDCl₃) δ 1.58-1.70 (m, 2H), 1.86-2.10 (m, 9H), 2.15-2.22 (m, 2H), 2.95 (s, 3H), 3.59-3.71 (m, 2H), 3.78 (s, 3H), 4.18-4.25 (m, 1H), 5.58 (bs, 1H), 5.73 (bs, 1H), 7.07-7.13 (m, 1H)

Example 42

¹H-NMR (CDCl₃) δ 1.58-1.70 (m, 2H), 1.88-2.12 (m, 9H), 2.14-2.21 (m, 2H), 2.86 (s, 3H), 3.42-3.51 (m, 2H), 3.78 (s, 3H), 4.15-4.23 (m, 1H), 5.34 (bs, 1H), 5.63 (bs, 1H), 6.48 (s, 1H), 7.13-7.23 (m, 1H)

Example 43

¹H-NMR (CDCl₃) δ 0.95-1.04 (m, 3H), 1.58-1.69 (m, 2H), 1.88-2.12 (m, 9H), 2.13-2.22 (m, 2H), 2.95-3.04 (m, 2H), 3.05-3.13 (m, 2H), 3.30 (s, 3H), 3.32-3.40 (m, 2H), 3.75 (s, 3H), 4.17-4.24 (m, 1H), 5.38 (bs, 1H), 5.65 (bs, 1H), 6.47 (s, 1H), 7.15-7.24 (m, 1H)

[Chemical Formula74]

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Example

No.
R_A
R_B
R_C
R_D

44

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CH₃
H

45
CH₂CH₃

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CH₃
Cl

46

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CH₃
Cl

47
CH₃
CH₂—CF₃
CH₂CH₃
H

48
CH₃
CH₂—CF₃
CH₃
CH₃

49
CH₃
CH₂—CF₃
CH₂CH₃
Cl

50
CH₂—CF₃

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CH₃
Cl

51
CH₂—CF₃

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CH₃
Cl

52
CH₂—CF₃

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CH₃
H

53
CH₂—CF₃

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CH₃
H

54
CH₃
CH₂—CF₃
CH₂CH₃
Cl

55
CH₂—CF₃

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CH₃
H

56
CH₂—CF₃

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CH₃
Cl

Example 44

¹H-NMR (CDCl₃) δ 1.00-1.12 (m, 6H), 1.58-1.69 (m, 2H), 1.89-2.12 (m, 9H), 2.13-2.23 (m, 2H), 3.02-3.12 (m, 2H), 3.13-3.31 (m, 6H), 3.75 (s, 3H), 4.18-4.25 (m, 1H), 5.35 (bs, 1H), 5.64 (bs, 1H), 6.52 (s, 1H), 7.18-7.27 (m, 1H)

Example 45

¹H-NMR (CDCl₃) δ 0.95-1.04 (m, 3H), 1.58-1.69 (m, 2H), 1.85-2.10 (m, 9H), 2.15-2.22 (m, 2H), 3.15-3.22 (m, 2H), 3.28-3.37 (m, 7H), 3.75 (s, 3H), 4.18-4.25 (m, 1H), 5.30 (bs, 1H), 5.64 (bs, 1H), 7.15-7.27 (m, 1H)

Example 46

¹H-NMR (CDCl₃) δ 1.05-1.16 (m, 6H), 1.58-1.68 (m, 2H), 1.88-2.10 (m, 9H), 2.18-2.24 (m, 2H), 3.22-3.28 (m, 5H), 3.29-3.48 (m, 3H), 3.75 (s, 3H), 4.18-4.25 (m, 1H), 5.32 (bs, 1H), 5.65 (bs, 1H), 7.05-7.16 (m, 1H)

Example 47

¹H-NMR (CDCl₃) δ 1.38-1.48 (m, 3H), 1.59-1.70 (m, 2H), 1.88-2.11 (m, 9H), 2.14-2.21 (m, 2H), 2.84 (s, 3H), 3.40-3.52 (m, 2H), 4.07-4.24 (m, 3H), 5.32 (bs, 1H), 5.63 (bs, 1H), 6.50 (s, 1H), 7.18-7.25 (m, 1H)

Example 48

¹H-NMR (CDCl₃) δ 1.56-1.75 (m, 2H), 1.89-2.10 (m, 9H), 2.13-2.19 (m, 2H), 2.28 (s, 3H), 2.92 (s, 3H), 3.50-3.62 (m, 2H), 3.74 (s, 3H), 4.14-4.22 (m, 1H), 5.32 (bs, 1H), 5.62 (bs, 1H), 7.18-7.29 (m, 1H)

Example 49

¹H-NMR (CDCl₃) δ 1.38-1.50 (m, 3H), 1.58-1.72 (m, 2H), 1.86-2.11 (m, 9H), 2.15-2.25 (m, 2H), 2.95 (s, 3H), 3.60-3.72 (m, 2H), 4.08-4.26 (m, 3H), 5.33 (bs, 1H), 5.64 (bs, 1H), 7.05-7.14 (m, 1H)

Example 50

¹H-NMR (CDCl₃) δ 1.62-1.69 (m, 2H), 1.88-2.11 (m, 9H), 2.15-2.24 (m, 2H), 3.27-3.45 (m, 7H), 3.72-3.87 (m, 5H), 4.16-4.27 (m, 1H), 5.32 (bs, 1H), 5.64 (bs, 1H), 7.06-7.14 (m, 1H)

Example 51

¹H-NMR (CDCl₃) δ 1.58-1.72 (m, 4H), 1.85-2.12 (m, 9H), 2.13-2.22 (m, 2H), 3.23-3.36 (m, 5H), 3.36-3.46 (m, 2H), 3.63-3.72 (m, 2H), 3.77 (s, 3H), 4.16-4.25 (m, 1H), 5.29 (bs, 1H), 5.62 (bs, 1H), 7.05-7.14 (m, 1H)

Example 52

¹H-NMR (CDCl₃) δ 1.59-1.70 (m, 2H), 1.88-2.11 (m, 9H), 2.14-2.21 (m, 2H), 3.18-3.25 (m, 2H), 3.32 (s, 3H), 3.36-3.43 (m, 2H), 3.61-3.72 (m, 2H), 3.77 (s, 3H), 4.17-4.23 (m, 1H), 5.33 (bs, 1H), 5.63 (bs, 1H), 6.55 (s, 1H), 7.16-7.25 (m, 1H)

Example 53

¹H-NMR (CDCl₃) δ 1.59-1.72 (m, 4H), 1.89-2.11 (m, 9H), 2.12-2.22 (m, 2H), 3.09-3.11 (m, 2H), 3.30 (s, 3H), 3.33-3.41 (m, 2H), 3.45-3.66 (m, 2H), 3.77 (s, 3H), 4.16-4.25 (m, 1H), 5.35 (bs, 1H), 5.63 (bs, 1H), 6.54 (s, 1H), 7.16-7.23 (m, 1H)

Example 54

¹H-NMR (CDCl₃) δ 1.39-1.46 (m, 3H), 1.59-1.68 (m, 2H), 1.85-2.20 (m, 12H), 2.95 (s, 3H), 3.61-3.71 (m, 2H), 4.08-4.17 (m, 2H), 4.18-4.25 (m, 1H), 7.08-7.14 (m, 1H)

Example 55

¹H-NMR (CDCl₃) δ 1.05-1.14 (m, 6H), 1.61-1.68 (m, 2H), 1.90-2.10 (m, 9H), 2.15-2.21 (m, 2H), 3.18-3.28 (m, 1H), 3.41-3.52 (m, 2H), 3.77 (s, 3H), 4.17-4.24 (m, 1H), 5.31 (bs, 1H), 5.62 (bs, 1H), 6.54 (s, 1H), 7.15-7.27 (m, 1H)

Example 56

¹H-NMR (CDCl₃) δ 1.08-1.21 (m, 6H), 1.60-1.68 (m, 2H), 1.89-2.10 (m, 9H), 2.18-2.22 (m, 2H), 3.38-3.48 (m, 1H), 3.50-3.82 (m, 5H), 4.18-4.24 (m, 1H), 5.30 (bs, 1H), 5.63 (bs, 1H), 7.08-7.17 (m, 1H)

[Chemical Formula 75]

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Ex-

am-

ple

No.
R_B
R_D
R¹
R²

57
CH₃
Cl
H

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58
CH₃
Cl
H

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Cl
H

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62
CH₂—CF₃
CH₃
CH₂CH₃
CH₂CH₃

Example 57

¹H-NMR (CDCl₃) δ 1.62-1.65 (m, 2H), 1.75 (m, 1H), 1.89-1.95 (m, 4H), 2.05-2.10 (m, 4H), 2.15-2.28 (m, 2H), 2.84 (s, 6H), 3.71 (s, 3H), 4.21 (m, 1H), 4.45 (d, J=4.0 Hz, 2H), 6.24 (m, 1H), 7.07 (m, 1H), 7.14 (d, J=8.0 Hz, 2H), 8.53 (d, J=8.0 Hz, 2H)

Example 58

¹H-NMR (CDCl₃) δ 1.60-1.63 (m, 2H), 1.80-1.87 (m, 5H), 2.01-2.05 (m, 4H), 2.13-2.15 (m, 2H), 2.84 (s, 6H), 3.71 (s, 3H), 4.18 (m, 1H), 4.45 (d, J=4.0 Hz, 2H), 6.21 (m, 1H), 7.07 (d, J=8.0 Hz, 1H), 7.25 (d, J=8.0 Hz, 1H), 7.59 (d, J=8.0 Hz, 1H), 8.49-8.51 (m, 2H)

Example 59

¹H-NMR (CDCl₃) δ 0.90 (t, J=8.0 Hz, 3H), 1.46 (q, J=8.0 Hz, 2H), 1.62-1.65 (m, 2H), 1.88-1.91 (m, 5H), 2.01-2.07 (m, 5H), 2.15-2.17 (m, 2H), 2.81 (s, 3H), 3.04 (t, J=8.0 Hz, 2H), 3.46-3.50 (m, 2H), 3.73 (s, 3H), 3.76-3.80 (m, 2H), 4.20 (m, 1H), 6.34 (m, 1H), 7.17 (m, 1H)

Example 60

¹H-NMR (CDCl₃) δ 0.90 (t, J=8.0 Hz, 3H), 1.44 (q, J=8.0 Hz, 2H), 1.62-1.65 (m, 2H), 1.86-1.93 (m, 4H), 1.98-2.08 (m, 4H), 2.15-2.20 (m, 3H), 2.81 (s, 3H), 3.04 (t, J=8.0 Hz, 2H), 3.37 (s, 3H), 3.42-3.50 (m, 4H), 3.72 (s, 3H), 4.21 (m, 1H), 6.12 (m, 1H), 7.15 (m, 1H)

Example 61

¹H-NMR (CDCl₃) δ 1.10 (d, J=4.0 Hz, 6H), 1.60-1.63 (m, 2H), 1.86-1.95 (m, 8H), 2.05 (m, 1H), 2.13-2.16 (m, 2H), 2.79 (s, 3H), 3.28-3.31 (m, 2H), 3.38 (m, 1H), 3.68-3.72 (m, 5H), 4.18 (m, 1H), 6.51 (m, 1H), 7.10 (m, 1H), 7.57-7.62 (m, 2H), 7.70 (m, 1H), 7.90-7.92 (m, 2H)

Example 62

¹H-NMR (CDCl₃) δ 1.08-1.17 (m, 6H), 1.58-1.70 (m, 2H), 1.86-1.95 (m, 2H), 2.01-2.25 (m, 9H), 2.28 (s, 3H), 2.92 (s, 3H), 3.36-3.50 (m, 4H), 3.52-3.61 (m, 2H), 3.74 (s, 3H), 4.18-4.25 (m, 1H), 7.22-7.29 (m, 1H)

[Chemical Formula 76]

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Ex-

am-

ple

No.
R_A
R_B
R_D
A

63

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CH₃
H
CONH₂

64

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CH₃
Cl
OH

65

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CH₃
Cl
CONH₂

66

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CH₃
Cl
CONH₂

67

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CH2—CF3
Cl
OH

68

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H
CONH₂

69

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H
CONH₂

70

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Cl
OH

71

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Cl
OH

Example 63

¹H-NMR (CDCl₃) δ 1.62-1.66 (m, 2H), 1.93-2.08 (m, 9H), 2.15-2.18 (m, 2H), 2.61 (s, 3H), 2.87-2.92 (m, 2H), 3.07-3.12 (m, 2H), 3.75 (s, 3H), 3.90 (m, 1H), 4.22 (m, 1H), 5.17 (bs, 1H), 5.58 (bs, 1H), 6.51 (s, 1H), 7.13-7.19 (m, 5H)

Example 64

¹H-NMR (CDCl₃) δ 1.53-1.62 (m, 4H), 1.78-1.95 (m, 7H), 2.17-2.23 (m, 3H), 2.77-2.79 (m, 2H), 2.84 (s, 3H), 3.06-3.12 (m, 2H), 3.64 (s, 3H), 4.19 (m, 1H), 4.30 (m, 1H), 7.04 (m, 1H), 7.15-7.20 (m, 4H)

Example 65

¹H-NMR (CDCl₃) δ 1.63-1.69 (m, 2H), 1.89-1.95 (m, 4H), 2.02-2.07 (m, 5H), 2.15-2.19 (m, 2H), 2.77-2.82 (m, 2H), 2.84 (s, 3H), 3.06-3.11 (m, 2H), 3.64 (s, 3H), 4.23 (m, 1H), 4.32 (m, 1H), 5.36 (bs, 1H), 5.66 (bs, 1H), 7.11-7.15 (m, 5H)

Example 66

¹H-NMR (CDCl₃) δ 0.05-0.10 (m, 2H), 0.39-0.48 (m, 2H), 0.79-0.90 (m, 1H), 1.58-1.68 (m, 2H), 1.88-2.09 (m, 9H), 2.16-2.23 (m, 2H), 2.86 (s, 3H), 2.86-2.93 (m, 2H), 3.75 (s, 3H), 4.17-4.23 (m, 1H), 5.46 (bs, 1H), 5.71 (bs, 1H), 7.07-7.14 (m, 1H)

Example 67

¹H-NMR (CDCl₃) δ 0.52-0.68 (m, 4H), 1.42-1.49 (m, 1H), 1.51-1.60 (m, 2H), 1.73-1.98 (m, 8H), 2.15-2.27 (m, 3H), 3.00-3.08 (m, 1H), 3.68-3.82 (m, 5H), 4.16-4.25 (m, 1H), 7.00-7.08 (m, 1H)

Example 68

¹H-NMR (CDCl₃) δ 0.44-0.45 (m, 2H), 0.63-0.64 (m, 2H), 1.61-1.66 (m, 2H), 1.75-1.82 (m, 2H), 1.89-2.18 (m, 11H), 2.49-2.54 (m, 1H), 3.14-3.18 (m, 2H), 3.28 (s, 3H), 3.34-3.37 (m, 2H), 3.75-3.76 (m, 3H), 4.20-4.21 (m, 1H), 5.27-5.29 (m, 1H), 5.61-5.66 (m, 1H), 6.48 (s, 1H), 7.44-7.52 (m, 1H)

Example 69

¹H-NMR (CDCl₃) δ 0.45-0.46 (m, 2H), 0.60-0.63 (m, 2H), 1.61-1.64 (m, 2H), 1.89-2.07 (m, 8H), 2.18 (bs, 3H), 2.58-2.59 (m, 1H), 3.22-3.26 (m, 2H), 3.29 (s, 3H), 3.44-3.47 (m, 2H), 3.74 (s, 3H), 4.20-4.22 (m, 1H), 5.28 (bs, 1H), 5.63 (bs, 1H), 6.51 (s, 1H), 7.40-7.42 (m, 1H)

Example 70

¹H-NMR (CDCl₃) δ 0.38-0.41 (m, 2H), 0.54-0.58 (m, 2H), 1.68-1.82 (m, 10H), 1.91-1.94 (m, 2H), 2.14-2.23 (m, 4H), 2.90-2.95 (m, 1H), 3.23-3.27 (m, 2H), 3.30 (s, 3H), 3.36-3.39 (m, 2H), 3.65 (s, 3H), 4.19-4.21 (m, 1H), 7.03-7.05 (m, 1H)

Example 71

¹H-NMR (CDCl₃) δ 0.41-0.44 (m, 2H), 0.54-0.58 (m, 2H), 1.50-1.61 (m, 2H), 1.73-1.86 (m, 7H), 1.89-1.94 (m, 2H), 2.17-2.25 (m, 3H), 2.95-3.00 (m, 1H), 3.28 (s, 3H), 3.35-3.39 (m, 4H), 3.69 (s, 3H), 4.17-4.22 (m, 1H), 7.05-7.08 (m, 1H)

[Chemical Formula 77]

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Ex-

ample

No.
R_A
R_B
R_D
A

72

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H
CONH₂

73

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Cl
OH

74

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Cl
CONH₂

75

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H
CONH₂

76

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Cl
OH

77

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Cl
CONH₂

78

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H
CONH₂

Example 72

¹H-NMR (CDCl₃) δ 1.57-1.66 (m, 2H), 1.71-1.81 (m, 3H), 1.91-2.17 (m, 14H), 2.54-2.58 (m, 2H), 3.05-3.09 (m, 2H), 3.63-3.69 (m, 4H), 3.78 (s, 3H), 4.22-4.24 (m, 1H), 5.43 (bs, 1H), 5.67 (bs, 1H), 6.53 (s, 1H), 6.78-6.81 (m, 2H), 6.97-7.00 (m, 2H), 7.32-7.35 (m, 1H)

Example 73

¹H-NMR (CDCl₃) δ 0.39-0.43 (m, 2H), 0.55-0.59 (m, 2H), 1.52-1.56 (m, 2H), 1.78-1.94 (m, 9H), 2.19-2.23 (m, 3H), 2.71-2.75 (m, 2H), 2.92-2.96 (m, 1H), 3.43-3.48 (m, 5H), 3.78 (s, 3H), 4.19-4.21 (m, 1H), 6.65-6.66 (m, 1H), 6.70-6.75 (m, 2H), 7.04-7.06 (m, 1H), 7.16-7.20 (m, 1H)

Example 74

¹H-NMR (CDCl₃) δ 0.40-0.44 (m, 2H), 0.55-0.60 (m, 2H), 1.64-1.67 (m, 2H), 1.82-2.20 (m, 11H), 2.72-2.76 (m, 2H), 2.91-2.96 (m, 1H), 3.44-3.47 (m, 2H), 3.50 (s, 3H), 3.78 (s, 3H), 4.24-4.26 (m, 1H), 6.07 (bs, 1H), 6.55 (bs, 1H), 6.66-6.68 (m, 1H), 6.71-6.75 (m, 2H), 7.14-7.23 (m, 2H)

Example 75

¹H-NMR (CDCl₃) δ 0.42-0.46 (m, 2H), 0.56-0.63 (m, 2H), 1.62-1.65 (m, 2H), 1.93-2.18 (m, 11H), 2.51-2.57 (m, 1H), 2.73-2.77 (m, 2H), 3.25-3.29 (m, 2H), 3.61 (s, 3H), 3.78 (s, 3H), 4.20-4.22 (m, 1H), 5.33 (bs, 1H), 5.65 (bs, 1H), 6.52 (s, 1H), 6.65-6.66 (m, 1H), 6.70-6.75 (m, 2H), 7.17-7.21 (m, 1H), 7.28-7.30 (m, 1H)

Example 76

¹H-NMR (CDCl₃) δ 0.39-0.42 (m, 2H), 0.54-0.59 (m, 2H), 1.52-1.56 (m, 2H), 1.74-1.95 (m, 9H), 2.19-2.24 (m, 3H), 2.67-2.71 (m, 2H), 2.91-2.96 (m, 1H), 3.40-3.44 (m, 2H), 3.48 (s, 3H), 3.78 (s, 3H), 4.19-4.21 (m, 1H), 6.79-6.86 (m, 2H), 7.02-7.06 (m, 3H)

Example 77

¹H-NMR (CDCl₃) δ 0.39-0.43 (m, 2H), 0.55-0.59 (m, 2H), 1.64-1.67 (m, 2H), 1.89-2.20 (m, 11H), 2.67-2.71 (m, 2H), 2.90-2.95 (m, 1H), 3.40-3.44 (m, 2H), 3.50 (s, 3H), 3.78 (s, 3H), 4.24-4.25 (m, 1H), 5.88 (bs, 1H), 6.34 (bs, 1H), 6.79-6.83 (m, 2H), 7.02-7.05 (m, 2H), 7.13-7.15 (m, 1H)

Example 78

¹H-NMR (CDCl₃) δ 0.42-0.45 (m, 2H), 0.59-0.62 (m, 2H), 1.62-1.65 (m, 2H), 1.93-2.19 (m, 11H), 2.51-2.55 (m, 1H), 2.67-2.74 (m, 2H), 3.23-3.27 (m, 2H), 3.62 (s, 3H), 3.78 (s, 3H), 4.21-4.23 (m, 1H), 5.30 (bs, 1H), 5.63 (bs, 1H), 6.50 (s, 1H), 6.79-6.83 (m, 2H), 7.01-7.05 (m, 2H), 7.33-7.35 (m, 1H)

[Chemical Formula 78]

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Example No.
R_B
R_D
A

79

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Cl
CONH₂

80

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Cl
OH

81

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H
CONH₂

82

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Cl
OH

83

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Cl
CONH₂

84

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H
OH

85

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H
CONH₂

86

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H
CONH₂

Example 79

¹H-NMR (CDCl₃) δ 0.43-0.46 (m, 2H), 0.58-0.61 (m, 2H), 1.62-1.65 (m, 2H), 1.88-1.93 (m, 3H), 1.99-2.10 (m, 5H), 2.17-2.18 (m, 3H), 2.96-3.01 (m, 1H), 3.28 (s, 3H), 3.77 (s, 3H), 4.23 (m, 3H), 5.89 (bs, 1H), 6.35 (bs, 1H), 6.75-6.79 (m, 2H), 7.03-7.05 (m, 2H), 7.08-7.10 (m, 1H)

Example 80

¹H-NMR (CDCl₃) δ 0.43-0.46 (m, 2H), 0.56-0.61 (m, 2H), 1.51-1.54 (m, 2H), 1.72-1.83 (m, 7H), 1.91-1.94 (m, 2H), 2.17-2.22 (m, 3H), 2.97-3.02 (m, 1H), 3.26 (s, 3H), 3.76 (s, 3H), 4.17-4.19 (m, 1H), 4.23 (s, 2H), 6.74-6.78 (m, 2H), 6.99-7.01 (m, 1H), 7.02-7.06 (m, 2H)

Example 81

¹H-NMR (CDCl₃) δ 0.43-0.47 (m, 2H), 0.56-0.61 (m, 2H), 1.61-1.64 (m, 2H), 1.88-1.92 (m, 3H), 1.99-2.08 (m, 5H), 2.17-2.18 (m, 3H), 2.39-2.44 (m, 1H), 3.58 (s, 3H), 3.78 (s, 3H), 4.09 (s, 2H), 4.19-4.21 (m, 1H), 5.31 (bs, 1H), 5.64 (bs, 1H), 6.45 (s, 1H), 6.77-6.81 (m, 2H), 7.00-7.05 (m, 2H), 7.34-7.36 (m, 1H)

Example 82

¹H-NMR (CDCl₃) δ 0.47-0.51 (m, 2H), 0.60-0.65 (m, 2H), 1.52-1.56 (m, 3H), 1.73-1.83 (m, 6H), 1.91-1.94 (m, 2H), 2.18-2.22 (m, 3H), 2.97-3.02 (m, 1H), 3.04 (s, 3H), 3.35 (s, 3H), 4.16-4.18 (m, 1H), 4.40 (s, 2H), 6.99-7.00 (m, 1H), 7.38-7.39 (m, 2H), 7.84-7.86 (m, 2H)

Example 83

¹H-NMR (CDCl₃) δ 0.49-0.54 (m, 2H), 0.62-0.64 (m, 2H), 1.65-2.18 (m, 13H), 2.99-3.01 (m, 1H), 3.04 (s, 3H), 3.36 (s, 3H), 4.19-4.21 (m, 1H), 4.40 (s, 2H), 5.35 (bs, 1H), 5.69 (bs, 1H), 7.06-7.08 (m, 1H), 7.38-7.40 (m, 2H), 7.84-7.86 (m, 2H)

Example 84

¹H-NMR (CDCl₃) δ 0.46-0.50 (m, 2H), 0.58-0.62 (m, 2H), 1.52-1.55 (m, 2H), 1.78-1.94 (m, 9H), 2.19-2.21 (m, 3H), 2.42-2.45 (m, 1H), 3.05 (s, 3H), 3.58 (s, 3H), 4.15-4.17 (m, 1H), 4.21 (s, 2H), 6.45 (s, 1H), 7.09-7.11 (m, 1H), 7.34-7.36 (m, 2H), 7.84-7.86 (m, 2H)

Example 85

¹H-NMR (CDCl₃) δ 0.48-0.50 (m, 2H), 0.58-0.61 (m, 2H), 1.62-1.65 (m, 2H), 1.92-2.07 (m, 9H), 2.17 (bs, 2H), 2.43-2.46 (m, 1H), 3.05 (s, 3H), 3.59 (s, 3H), 4.18-4.20 (m, 1H), 4.22 (s, 2H), 5.66 (bs, 2H), 6.45 (s, 1H), 7.19-7.21 (m, 1H), 7.34-7.36 (m, 2H), 7.84-7.87 (m, 2H)

Example 86

¹H-NMR (CDCl₃) δ 0.40-0.44 (m, 2H), 0.56-0.61 (m, 2H), 1.62-1.65 (m, 2H), 1.93-2.18 (m, 11H), 2.48-2.53 (m, 1H), 2.71-2.75 (m, 2H), 3.21-3.26 (m, 2H), 3.58 (s, 3H), 4.20-4.22 (m, 1H), 5.44 (bs, 1H), 5.67 (bs, 1H), 6.51 (s, 1H), 7.03-7.05 (m, 2H), 7.22-7.24 (m, 3H)

[Chemical Formula 79]

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Example No.
R_B
A

87

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CONH₂

88

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CONH₂

89

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OH

90

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OH

91

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OH

92

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OH

93

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CONH₂

94

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CONH₂

Example 87

¹H-NMR (CDCl₃) δ 1.62-1.66 (m, 2H), 1.89-1.93 (m, 4H), 2.03-2.09 (m, 5H), 2.15-2.18 (m, 2H), 2.96 (s, 3H), 3.57-3.59 (m, 2H), 3.75 (s, 3H), 3.91 (s, 3H), 4.02-4.04 (m, 2H), 4.20 (m, 1H), 5.78 (bs, 1H), 6.20 (bs, 1H), 6.44 (s, 1H), 7.12 (m, 1H), 7.35 (s, 1H)

Example 88

¹H-NMR (CDCl₃) δ 1.62-1.66 (m, 2H), 1.89-1.93 (m, 4H), 2.03-2.10 (m, 5H), 2.17-2.19 (m, 2H), 2.93 (s, 3H), 3.47-3.50 (m, 2H), 3.73 (s, 3H), 3.76 (s, 3H), 3.91-3.93 (m, 2H), 4.21 (m, 1H), 5.94 (bs, 1H), 6.35 (bs, 1H), 6.76-6.83 (m, 4H), 7.13 (m, 1H)

Example 89

¹H-NMR (CDCl₃) δ 1.52-1.60 (m, 4H), 1.77-1.84 (m, 5H), 1.91-1.94 (m, 2H), 2.18-2.22 (m, 3H), 2.92 (s, 3H), 3.47-3.50 (m, 2H), 3.73 (s, 3H), 3.76 (s, 3H), 3.90-3.92 (m, 2H), 4.18 (m, 1H), 6.75-6.83 (m, 4H), 7.01 (m, 1H)

Example 90

¹H-NMR (CDCl₃) δ 1.51-1.54 (m, 2H), 1.77-1.84 (m, 6H), 1.90-1.93 (m, 2H), 2.17-2.22 (m, 4H), 2.92 (s, 3H), 3.52-3.55 (m, 2H), 3.76 (s, 3H), 4.01-4.04 (m, 2H), 4.18 (m, 1H), 6.87-6.92 (m, 2H), 7.02-7.09 (m, 3H)

Example 91

¹H-NMR (CDCl₃) δ 1.53-1.56 (m, 2H), 1.78-1.83 (m, 5H), 1.91-1.94 (m, 2H), 2.18-2.23 (m, 2H), 2.33-2.36 (m, 3H), 2.92 (s, 3H), 3.50-3.52 (m, 2H), 3.72 (s, 3H), 3.94-3.97 (m, 2H), 4.18 (m, 1H), 6.53-6.66 (m, 3H), 7.06 (m, 1H), 7.22 (m, 1H)

Example 92

¹H-NMR (CDCl₃) δ 1.52-1.55 (m, 2H), 1.77-1.84 (m, 7H), 1.91-1.94 (m, 2H), 2.18-2.22 (m, 3H), 2.92 (s, 3H), 3.48-3.51 (m, 2H), 3.72 (s, 3H), 3.91-3.95 (m, 2H), 4.39 (m, 1H), 6.75-6.78 (m, 2H), 6.93-6.98 (m, 3H)

Example 93

¹H-NMR (CDCl₃) δ 1.61-1.64 (m, 2H), 1.88-1.92 (m, 4H), 1.98-2.10 (m, 5H), 2.13-2.17 (m, 2H), 2.92 (s, 3H), 3.52-3.54 (m, 2H), 3.75 (s, 3H), 4.02-4.04 (m, 2H), 4.20 (m, 1H), 5.76 (bs, 1H), 6.12 (bs, 1H), 6.87-6.92 (m, 2H), 7.01-7.11 (m, 3H)

Example 94

¹H-NMR (CDCl₃) δ 1.62-1.66 (m, 2H), 1.85-1.93 (m, 4H), 2.02-2.10 (m, 5H), 2.15-2.18 (m, 2H), 2.93 (s, 3H), 3.47-3.51 (m, 2H), 3.78 (s, 3H), 3.92-3.98 (m, 2H), 4.21 (m, 1H), 5.87 (bs, 1H), 6.30 (bs, 1H), 6.75-6.78 (m, 2H), 6.93-6.98 (m, 2H), 7.12 (m, 1H).

[Chemical Formula 80]

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Example No.
R_B
R_D
A

95

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H
CONH₂

96

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Cl
OH

97

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Cl
CONH₂

98

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Cl
OH

99

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H
CONH₂

100

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H
CONH₂

101

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Cl
CONH₂

Example 95

¹H-NMR (CDCl₃) δ 1.61-1.64 (m, 2H), 1.92-2.07 (m, 9H), 2.15-2.18 (m, 2H), 2.79 (s, 3H), 3.30 (t, J=4.0 Hz, 2H), 3.77 (s, 3H), 4.01 (t, J=4.0 Hz, 2H), 4.20 (m, 1H), 5.53 (bs, 1H), 5.77 (bs, 1H), 6.44 (s, 1H), 6.76-6.79 (m, 2H), 7.21-7.24 (m, 2H), 7.28 (m, 1H)

Example 96

¹H-NMR (CDCl₃) δ 1.46-1.56 (m, 2H), 1.60-1.71 (m, 2H), 1.75-1.89 (m, 6H), 1.91-1.95 (m, 2H), 2.15-2.26 (m, 2H), 2.92 (s, 3H), 3.49-3.51 (t, J=4.0 Hz, 2H), 3.71 (s, 3H), 3.92-3.94 (t, J=4.0 Hz, 2H), 4.20 (m, 1H), 6.75 (d, J=8.0 Hz, 2H), 7.02 (m, 1H), 7.21 (d, J=8.0 Hz, 2H)

Example 97

¹H-NMR (CDCl₃) δ 1.62-1.65 (m, 2H), 1.89-1.99 (m, 4H), 2.02-2.07 (m, 5H), 2.15-2.18 (m, 2H), 2.92 (s, 3H), 3.47-3.52 (m, 2H), 3.71 (s, 3H), 3.92-3.95 (m, 2H), 4.20 (m, 1H), 5.57 (bs, 1H), 5.93 (bs, 1H), 6.74-6.76 (m, 2H), 7.09 (m, 1H), 7.20-7.23 (m, 2H)

Example 98

¹H-NMR (CDCl₃) δ 1.48-1.96 (m, 11H), 2.16-2.28 (m, 3H), 2.85 (s, 3H), 3.06 (s, 3H), 3.65 (s, 3H), 4.15-4.22 (m, 1H), 4.37 (s, 2H), 6.95-7.03 (m, 1H), 7.48-7.56 (m, 2H), 7.88-7.94 (m, 2H)

Example 99

¹H-NMR (CDCl₃) δ 1.21-1.29 (m, 3H), 1.59-1.68 (m, 2H), 1.90-2.11 (m, 9H), 2.16-2.22 (m, 2H), 2.49-2.58 (m, 2H), 2.60-2.68 (m, 2H), 2.70 (s, 3H), 3.05-3.14 (m, 2H), 3.77 (s, 3H), 4.15-4.24 (m, 1H), 5.34 (bs, 1H), 5.63 (bs, 1H), 6.39 (s, 1H), 7.15-7.23 (m, 1H)

Example 100

¹H-NMR (CDCl₃) δ 1.60-1.64 (m, 2H), 1.90-2.11 (m, 11H), 2.15-2.20 (m, 2H), 2.67 (s, 3H), 2.90 (s, 3H), 3.02-3.11 (m, 4H), 3.75 (s, 3H), 4.15-4.24 (m, 1H), 5.31 (bs, 1H), 5.62 (bs, 1H), 6.40 (s, 1H), 7.15-7.22 (m, 1H)

Example 101

¹H-NMR (CDCl₃) δ 1.58-1.68 (m, 2H), 1.85-2.11 (m, 11H), 2.15-2.22 (m, 2H), 2.83 (s, 3H), 2.91 (s, 3H), 3.02-3.11 (m, 2H), 3.26-3.35 (m, 2H), 3.75 (s, 3H), 4.16-4.25 (m, 1H), 5.27 (bs, 1H), 5.62 (bs, 1H), 7.05-7.14 (m, 1H)

Example 102
4-Chloro-5-[cyclopropyl(tetrahydro-2H-4-pyranyl)amino]-N-[(E)-5-hydroxy-2-adamantyl]-1-methyl-1H-pyrazole-3-carboxamide

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¹H-NMR (CDCl₃) δ 0.12-0.19 (m, 1H), 0.43-0.54 (m, 2H), 0.67-0.74 (m, 1H), 1.25-1.45 (m, 2H), 1.52-1.69 (m, 4H), 1.73-1.94 (m, 8H), 2.09-2.28 (m, 4H), 2.83-2.89 (m, 1H), 3.31-3.46 (m, 3H), 3.64 (s, 3H), 3.91-4.02 (m, 2H), 4.18-4.21 (m, 1H), 7.03-7.04 (m, 1H)

[Chemical Formula 82]

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Example No.
R_A
R_B
R_C
R_D

103

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CH₃
Cl

104

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CH₃
Cl

105

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CH₃
H

106

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CH₃
Cl

107

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CH₃
F

108

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CH₃
Cl

109

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CH₃
Cl

110
CH₃
CH₂CH₃
CH₃
F

111
CH₃
CH₂—CH₂—CF₃
CH₃
Cl

112
CH₃
CH₂—CH₂—CF₃
CH₃
F

[Chemical Formula 83]

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Example No.
R_A
R_B
R_C
R_D

113
CH₃
CH₂—CF₂—CF₃
CH₃
F

114
CH₃

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CH₃
H

115
CH₃

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CH₃
F

116
CH₃

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CH₃
F

117
CH₃
CH₂—CF₃
CH₃
F

118

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CH₃
F

119
CH₃
CH₂—CH₂—CH₃
CH₃
F

120
CH₃
CH₂—CHF₂
CH₃
F

121

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CH₃
F

122
CH₂CH₃

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CH₃
F

[Chemical Formula 84]

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Example No.
R_A
R_B
R_C
R_D

123
CH₃
CH₂—CHF₂
CH₃
Cl

124
CH₃

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CH₃
F

Example 103

¹H-NMR (CDCl₃) δ 0.36-0.39 (m, 2H), 0.52-0.56 (m, 2H), 1.60-1.63 (m, 2H), 1.66-1.73 (m, 2H), 1.88-2.16 (m, 11H), 2.87-2.92 (m, 1H), 3.21-3.25 (m, 2H), 3.28 (s, 3H), 3.35-3.38 (m, 2H), 3.64 (s, 3H), 4.18-4.20 (m, 1H), 5.94-6.02 (m, 2H), 7.09-7.11 (m, 1H)

Example 104

¹H-NMR (CDCl₃) δ 0.39-0.43 (m, 2H), 0.52-0.57 (m, 2H), 1.89-2.16 (m, 12H), 2.93-2.98 (m, 1H), 3.26 (s, 3H), 3.33-3.38 (m, 4H), 3.67 (s, 3H), 4.19-4.21 (m, 2H), 5.76-5.97 (m, 2H), 7.10-7.12 (m, 1H)

Example 105

¹H-NMR (CDCl₃) δ 0.42-0.46 (m, 4H), 0.55-0.60 (m, 4H), 1.61-1.64 (m, 2H), 1.92-2.16 (m, 11H), 2.53-2.58 (m, 2H), 3.68 (s, 3H), 4.18-4.20 (m, 1H), 5.44-5.68 (m, 2H), 6.42 (s, 1H), 7.24-7.28 (m, 1H)

Example 106

¹H-NMR (CDCl₃) δ 0.36-0.53 (m, 8H), 1.63-1.67 (m, 2H), 1.90-1.94 (m, 2H), 2.00-2.19 (m, 9H), 2.88-2.93 (m, 2H), 3.64 (s, 3H), 4.24-4.26 (m, 1H), 6.18 (bs, 1H), 6.74 (bs, 1H), 7.15-7.17 (m, 1H)

Example 107

¹H-NMR (CDCl₃) δ 0.39-0.43 (m, 2H), 0.55-0.59 (m, 2H), 1.63-1.66 (m, 2H), 1.71-2.11 (m, 10H), 2.14-2.18 (m, 3H), 2.73-2.75 (m, 1H), 3.14-3.18 (m, 2H), 3.30 (s, 3H), 3.37-3.40 (m, 2H), 3.63 (s, 3H), 4.21-4.25 (m, 1H), 5.67-5.74 (m, 1H), 6.13-6.18 (m, 1H), 6.92-6.94 (m, 1H)

Example 108

¹H-NMR (CDCl₃) δ 0.15-0.24 (m, 1H), 0.41-0.56 (m, 2H), 0.61-0.70 (m, 1H), 1.31-2.20 (m, 21H), 2.82-2.87 (m, 1H), 3.67 (s, 3H), 3.74-3.80 (m, 1H), 4.25-4.26 (m, 1H), 6.13-6.27 (m, 1H), 6.68-6.86 (m, 1H), 7.16-7.18 (m, 1H).

Example 109

¹H-NMR (CDCl₃) δ 0.13-0.20 (m, 1H), 0.45-0.55 (m, 2H), 0.67-0.75 (m, 1H), 1.51-1.71 (m, 6H), 1.73-2.20 (m, 11H), 2.84-2.89 (m, 1H), 3.32-3.45 (m, 3H), 3.68 (s, 3H), 3.92-4.03 (m, 2H), 4.23-4.25 (m, 1H), 6.11-6.27 (m, 1H), 6.57-6.70 (m, 1H), 7.15-7.17 (m, 1H)

Example 110

¹H-NMR (CDCl₃) δ 1.05-1.09 (m, 3H), 1.62-1.65 (m, 2H), 1.83-2.07 (m, 9H), 2.18 (brs, 2H), 2.78 (s, 3H), 3.00-3.05 (m, 2H), 3.69 (m, 3H), 4.19-4.23 (m, 1H), 5.23 (s, 1H), 5.62 (s, 1H), 6.90-6.93 (m, 1H)

Example 111

¹H-NMR (CDCl₃) δ 1.58-1.70 (m, 2H), 1.85-2.22 (m, 9H), 2.16-2.22 (m, 2H), 2.22-2.34 (m, 2H), 2.84 (s, 3H), 3.39-3.43 (m, 2H), 4.19-4.23 (m, 1H), 5.29 (s, 1H), 5.62 (s, 1H), 7.07-7.11 (m, 1H)

Example 112

¹H-NMR (CDCl₃) δ 1.55-1.67 (m, 2H), 1.83-2.10 (m, 12H), 2.11-2.19 (m, 2H), 2.20-2.36 (m, 2H), 2.77 (s, 3H), 3.24-3.29 (m, 2H), 3.67 (s, 3H), 4.16-4.23 (m, 1H), 5.42 (s, 1H), 5.80 (s, 1H), 6.87-6.95 (m, 1H)

Example 113

¹H-NMR (CDCl₃) δ 1.57-1.70 (m, 2H), 1.73-2.10 (m, 10H), 2.11-2.29 (m, 2H), 2.91 (s, 3H), 3.60-3.78 (m, 2H), 3.72 (s, 3H), 4.15-4.23 (m, 1H), 5.43 (s, 1H), 5.82 (s, 1H), 6.87-6.97 (m, 1H)

Example 114

¹H-NMR (CDCl₃) δ 1.55-1.78 (m, 6H), 1.93-2.07 (m, 9H), 2.17-2.18 (m, 2H), 2.62 (s, 3H), 2.87-2.95 (m, 1H), 3.31-3.37 (m, 2H), 3.75 (s, 3H), 3.97-4.00 (m, 2H), 4.20-4.21 (m, 1H), 5.26 (bs, 1H), 5.62 (bs, 1H), 6.48 (s, 1H), 7.22-7.24 (m, 1H)

Example 115

¹H-NMR (DMSO-d₆) δ 0.84-0.85 (m, 6H), 1.27-1.32 (m, 2H), 1.46-1.49 (m, 2H), 1.53-1.60 (m, 1H), 1.75-1.98 (m, 11H), 2.71 (s, 3H), 2.94-2.98 (m, 2H), 3.65 (s, 3H), 3.92-3.94 (m, 1H), 6.74 (s, 1H), 7.01 (s, 1H), 7.28-7.30 (m, 1H)

Example 116

¹H-NMR (CDCl₃) δ 1.62-1.66 (m, 2H), 1.89-2.08 (m, 10H), 2.18 (s, 2H), 2.84 (s, 3H), 3.17-3.19 (m, 2H), 3.33 (s, 3H), 3.42-3.44 (m, 2H), 3.72 (s, 3H), 4.21-4.25 (m, 1H), 5.43 (s, 1H), 5.75 (s, 1H), 6.92-6.94 (m, 1H)

Example 117

¹H-NMR (CDCl₃) δ 1.63-1.66 (m, 2H), 1.80-1.96 (m, 4H), 1.99-2.08 (m, 5H), 2.17-2.18 (m, 2H), 2.94 (s, 3H), 3.54-3.61 (m, 2H), 3.74 (s, 3H), 4.20-4.22 (m, 1H), 5.47 (bs, 1H), 5.85 (bs, 1H), 6.92-6.94 (m, 1H)

Example 118

¹H-NMR (CDCl₃) δ 0.41-0.45 (m, 2H), 0.54-0.59 (m, 2H), 1.62-1.66 (m, 2H), 1.90-1.93 (m, 4H), 1.99-2.07 (m, 5H), 2.18-2.19 (m, 2H), 2.79-2.83 (m, 1H), 3.25-3.28 (m, 2H), 3.29 (s, 3H), 3.40-3.43 (m, 2H), 3.64 (s, 3H), 4.22-4.24 (m, 1H), 5.23 (bs, 1H), 5.62 (bs, 1H), 6.92-6.94 (m, 1H)

Example 119

¹H-NMR (CDCl₃) δ 0.88-0.92 (m, 3H), 1.44-1.53 (m, 2H), 1.62-1.65 (m, 2H), 1.89-1.92 (m, 4H), 1.98-2.07 (m, 5H), 2.17-2.18 (m, 2H), 2.76 (s, 3H), 2.92-2.95 (m, 2H), 3.69 (s, 3H), 4.21-4.23 (m, 1H), 5.23 (bs, 1H), 5.62 (bs, 1H), 6.90-6.92 (m, 1H)

Example 120

¹H-NMR (CDCl₃) δ 1.62-1.66 (m, 2H), 1.88-1.92 (m, 4H), 1.98-2.07 (m, 5H), 2.17-2.18 (m, 2H), 2.89 (s, 3H), 3.33-3.47 (m, 2H), 3.73 (s, 3H), 4.20-4.22 (m, 1H), 5.19 (bs, 1H), 5.59 (bs, 1H), 5.69-5.99 (m, 1H), 6.91-6.93 (m, 1H)

Example 121

¹H-NMR (CDCl₃) δ 1.09-1.10 (m, 6H), 1.63-1.66 (m, 2H), 1.91-1.93 (m, 4H), 1.99-2.07 (m, 5H), 2.19 (bs, 2H), 3.21-3.28 (m, 8H), 3.71 (s, 3H), 4.22-4.24 (m, 1H), 5.23 (bs, 1H), 5.63 (bs, 1H), 6.94-6.96 (m, 1H)

Example 122

¹H-NMR (CDCl₃) δ 1.01-1.05 (m, 3H), 1.63-1.65 (m, 2H), 1.90-1.93 (m, 4H), 1.99-2.07 (m, 5H), 2.18 (bs, 2H), 3.07-3.13 (m, 2H), 3.19-3.23 (m, 2H), 3.29 (s, 3H), 3.35-3.38 (m, 2H), 3.71 (s, 3H), 4.22-4.23 (m, 1H), 5.32 (bs, 1H), 5.68 (bs, 1H), 6.93-6.95 (m, 1H)

Example 123

¹H-NMR (CDCl₃) δ 1.63-1.66 (m, 2H), 1.86-1.95 (m, 4H), 1.99-2.07 (m, 5H), 2.18 (bs, 2H), 2.92 (s, 3H), 3.43-3.50 (m, 2H), 3.77 (s, 3H), 4.20-4.22 (m, 1H), 5.22 (bs, 1H), 5.60 (bs, 1H), 5.67-5.97 (m, 1H), 7.08-7.10 (m, 1H)

Example 124

¹H-NMR (CDCl₃) δ 0.07-0.11 (m, 2H), 0.45-0.50 (m, 2H), 0.84-0.89 (m, 1H), 1.62-1.66 (m, 2H), 1.89-1.93 (m, 4H), 1.99-2.07 (m, 5H), 2.18 (bs, 2H), 2.81-2.83 (m, 5H), 3.72 (s, 3H), 4.22-4.23 (m, 1H), 5.24 (bs, 1H), 5.63 (bs, 1H), 6.91-6.93 (m, 1H)

[Chemical Formula 85]

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Example No.
R_A
R_B
R_C
R_D

125

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CH₃
Cl

126

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CH₃
F

127
CH₃

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CH₃
Cl

128
CH₃

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CH₃
F

129
CH₃

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CH₃
Cl

130
CH₃

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CH₃
F

131
CH₃

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CH₃
F

Example 125

Example 126

¹H-NMR (CDCl₃) δ 0.39-0.42 (m, 2H), 0.54-0.58 (m, 2H), 1.52-1.55 (m, 2H), 1.71-1.84 (m, 9H), 1.92-1.95 (m, 2H), 2.18-2.22 (m, 3H), 2.72-2.75 (m, 1H), 3.14-3.18 (m, 2H), 3.33 (s, 3H), 3.36-3.39 (m, 2H), 3.61 (s, 3H), 4.18-4.20 (m, 1H), 6.84-6.86 (m, 1H)

Example 127

¹H-NMR (DMSO-d₆) δ 1.36-1.39 (m, 2H), 1.60-1.78 (m, 9H), 1.93-2.00 (m, 4H), 2.10-2.16 (m, 2H), 2.22-2.29 (m, 2H), 2.40 (s, 3H), 3.33 (s, 3H), 3.48 (s, 2H), 3.84-3.86 (m, 1H), 4.45 (s, 1H), 7.13-7.16 (m, 1H), 7.21-7.31 (m, 5H)

Example 128

¹H-NMR (DMSO-d₆) δ 1.36-1.40 (m, 2H), 1.60-1.63 (m, 4H), 1.68-1.79 (m, 5H), 1.92-2.00 (m, 4H), 2.11-2.17 (m, 2H), 2.20-2.27 (m, 2H), 2.43 (s, 3H), 3.34 (s, 3H), 3.39 (s, 2H), 3.84-3.86 (m, 1H), 4.44 (bs, 1H), 7.04-7.06 (m, 1H), 7.09-7.13 (m, 1H), 7.16-7.18 (m, 2H), 7.24-7.28 (m, 2H)

Example 129

¹H-NMR (CDCl₃) δ 1.25-1.57 (m, 5H), 1.74-1.84 (m, 8H), 1.91-1.94 (m, 2H), 2.18-2.22 (m, 3H), 2.80 (s, 3H), 3.28-3.40 (m, 3H), 3.73 (s, 3H), 3.96-3.98 (m, 2H), 4.18-4.19 (m, 1H), 7.01-7.03 (m, 1H)

Example 130

¹H-NMR (CDCl₃) δ 1.53-1.56 (m, 4H), 1.78-1.84 (m, 9H), 1.92-1.95 (m, 2H), 2.18-2.22 (m, 3H), 2.75 (s, 3H), 3.04-0.311 (m, 1H), 3.34-3.40 (m, 2H), 3.69 (s, 3H), 3.97-4.00 (m, 2H), 4.18-4.20 (m, 1H), 6.85-6.87 (m, 1H)

Example 131

¹H-NMR (CDCl₃) δ 0.07-0.11 (m, 2H), 0.44-0.49 (m, 2H), 0.83-0.90 (m, 1H), 1.39 (bs, 1H), 1.52-1.55 (m, 2H), 1.77-1.84 (m, 6H), 1.92-1.94 (m, 2H), 2.18-2.22 (m, 3H), 2.81-2.86 (m, 5H), 3.71 (s, 3H), 4.18-4.20 (m, 1H), 6.83-6.85 (m, 1H)

Example 132
4-Chloro-N-[(E)-5-hydroxy-2-adamantyl]-1-methyl-5-{methyl[(3S)-piperidin-3-yl]amino}-1H-pyrazole-3-carboxamide

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Step (i):

To an ice-cooled solution of Compound (14.6 g) in THF (180 mL) was added sodium tertiary-butoxide (3.82 g), and the mixture was stirred for 1 hour. Then, thereto was slowly added methyl iodide (2.59 mL) at 0° C., and the mixture was stirred for 6 hours. Then, thereto was added saturated aqueous ammonium chloride solution, and then the mixture was extracted with ethyl acetate. The organic layer was washed with brine, and then dried over magnesium sulfate and concentrated in vacuo. The residue was purified by silica gel chromatography (eluent: hexane/ethyl acetate=1/1) to give Compound II (8.11 g).

Step (ii):

To a solution of Compound II (4.00 g) in DMF (30.0 mL) was added N-chlorosuccinimide (1.47 g), and the mixture was stirred at 60° C. for 3 hours. Then, thereto was added water, and then the mixture was extracted with ethyl acetate. The organic layer was washed with water and brine, and then dried over magnesium sulfate and concentrated in vacuo. The residue was purified by silica gel chromatography (eluent: hexane/ethyl acetate=1/1) to give Compound III (4.19 g).

Step (iii):

To a solution of Compound III (4.19 g) in ethanol (48.0 mL) was added 2N lithium hydroxide solution (14.4 mL), and the mixture was stirred at 50° C. for 2 hours. The reaction solution was concentrated in vacuo, and was adjusted to weak acidity by 1N hydrochloric acid and extracted with ethyl acetate. The organic layer was washed with brine, and then dried over magnesium sulfate and concentrated in vacuo to give Compound IV (3.61 g).

Step (iv):

To a solution of Compound IV (3.45 g) in DMF (80.0 mL) were added (E)-4-aminoadamantan-1-ol (1.70 g), WSCI.HCl (2.43 g), HOBt.H₂O (1.72 g) and triethylamine (3.54 mL) at room temperature, and the mixture was stirred overnight. Then, thereto was added saturated aqueous ammonium chloride solution, and then the mixture was extracted with ethyl acetate. The organic layer was washed with water and brine, and then dried over magnesium sulfate and concentrated in vacuo. The residue was purified by silica gel chromatography (eluent: chloroform/meththanol=20/1) to give Compound V (3.81 g).

Step (v):

To a solution of Compound V (3.80 g) in methanol (35.0 mL) was added 10% palladium-carbon (380 mg), and the mixture was stirred at ambient temperature and normal pressure under hydrogen atmosphere for 6 hours. The reaction solution was filtered, and then the filtrate was concentrated in vacuo to give the titled Compound VI (3.00 g) as a white solid.

¹H-NMR (CDCl₃) δ 1.22-1.36 (m, 1H), 1.43-1.58 (m, 1H), 1.49-1.59 (m, 2H), 1.66-1.88 (m, 7H), 1.88-1.98 (m, 3H), 2.14-2.27 (m, 3H), 2.46-2.58 (m, 2H), 2.81 (s, 3H), 2.94-3.03 (m, 1H), 3.13-3.27 (m, 2H), 3.74 (s, 3H), 4.16-4.22 (m, 1H), 7.03 (d, J=7 Hz, 1H)

Compounds of Examples 133-138 were prepared in the similar manner to Example 132.

Example 133
4-Chloro-N-[(E)-5hydroxy-2-adamantyl]-1-methyl-5-[methyl(piperidin-4-yl)amino]-1H-pyrazole-3-carboxamide

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¹H-NMR (CDCl₃) δ 1.35-1.58 (m, 4H), 1.61-2.10 (m, 12H), 2.10-2.27 (m, 3H), 2.58-2.65 (m, 2H), 2.78 (s, 3H), 3.11-3.15 (m, 2H), 3.70 (s, 3H), 4.15-4.19 (m, 1H), 6.99-7.02 (m, 1H)

Example 134
4-Chloro-N-[(E)-5-hydroxy-2-adamantyl]-1-methyl-5-[methyl(piperidin-4-ylmethyl)amino]-1H-pyrazole-3-carboxamide

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¹H-NMR (CDCl₃) δ 1.05-1.35 (m, 3H), 1.45-1.60 (m, 3H), 1.63-2.07 (m, 11H), 2.10-2.30 (m, 3H), 2.75 (m, 2H), 2.68-2.90 (s, 3H), 2.91-3.03 (m, 1H), 3.05-3.16 (m, 2H), 3.71 (s, 3H), 4.10-4.21 (m, 1H), 6.95-7.06 (m, 1H)

Example 135
4-Chloro-N-[(E)-5-hydroxy-2-adamantyl]-1-methyl-5-[methyl(2-piperidin-4-ylethyl)amino]-1H-pyrazole-3-carboxamide

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¹H-NMR (CDCl₃) δ 1.08-1.56 (m, 11H), 1.78-1.94 (m, 10H), 2.18-2.23 (m, 3H), 2.786-2.792 (m, 3H), 2.88-2.97 (m, 2H), 3.10-3.13 (m, 2H), 3.70-3.71 (m, 3H), 4.19-4.20 (m, 1H), 7.01-7.03 (m, 1H)

Example 136
4-Chloro-N-[(E)-5-hydroxy-2-adamantyl]-1-methyl-5-{methyl[(3R)-pyrrolidin-3-yl]amino}-1H-pyrazole-3-carboxamide

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¹H-NMR (CDCl₃) δ 1.52-1.56 (m, 2H), 1.78-1.85 (m, 8H), 1.92-1.94 (m, 2H), 2.06-2.11 (m, 1H), 2.21 (bs, 3H), 2.63-2.75 (m, 4H), 3.03-3.28 (m, 4H), 3.74 (s, 3H), 3.92-3.95 (m, 1H), 4.15-4.17 (m, 1H), 6.87-6.89 (m, 1H)

Example 137
4-Chloro-N-[(E)-5-hydroxy-2-adamantyl]-1-methyl-5-{methyl[(3S)-pyrrolidin-3-yl]amino}-1H-pyrazole-3-carboxamide

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¹H-NMR (CDCl₃) δ 1.53-1.97 (m, 13H), 2.12-2.21 (m, 4H), 2.81 (s, 3H), 3.34-3.43 (m, 4H), 3.83 (s, 3H), 4.15-4.17 (m, 1H), 4.32-4.37 (m, 1H), 7.03-7.05 (m, 1H)

Example 138
4-Chloro-N-[(E)-5-hydroxy-2-adamantyl]-1-methyl-5-{methyl[(3R)-piperidin-3-yl]amino}-1H-pyrazole-3-carboxamide

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¹H-NMR (CDCl₃) δ 1.23-1.32 (m, 1H), 1.40-1.73 (m, 7H), 1.78-1.85 (m, 6H), 1.92-1.94 (m, 3H), 2.18-2.23 (m, 3H), 2.45-2.52 (m, 2H), 2.81 (s, 3H), 3.09-3.20 (m, 2H), 3.73 (s, 3H), 4.18-4.20 (m, 1H), 7.01-7.03 (m, 1H)

Example 139
4-Fluoro-N-[(E)-5-hydroxy-2-adamantyl]-1-methyl-5-{methyl[(3S)-piperidin-3-yl]amino}-1H-pyrazole-3-carboxamide

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Step (i):

To an ice-cooled solution of Compound I (4.14 g) in DMF (35.0 mL) was added dropwise an aqueous solution (35.0 mL) of 1-chloromethyl-4-fluoro-1,4-diazoniabicyclo[2.2.2.] octane-bis(tetrafluoroborate) (5.76 g), and the mixture was stirred at room temperature for 15 hours. Then, thereto was added water, and then the mixture was extracted with ethyl acetate. The organic layer was washed with water and brine, and then dried over magnesium sulfate and concentrated in vacuo. The residue was purified by silica gel chromatography (eluent: hexane/ethyl acetate=1/1) to give Compound II (2.28 g).

Step (ii):

A mixed solution of Compound II (2.28 g), ethanol (27.3 mL) and 2N lithium hydroxide solution (8.15 mL) was stirred at 50° C. for 2 hours. The reaction solution was concentrated in vacuo, and was adjusted to weak acidity by 1N hydrochloric acid and extracted with ethyl acetate. The organic layer was washed with brine, and then dried over magnesium sulfate and concentrated in vacuo to give Compound III (2.00 g).

Step (iii):

A mixed solution of Compound III (2.00 g), (E)-4-aminoadamantan-1-ol (1.03 g), WSCI.HCl (1.47 g), HOBt.H₂O (1.04 g), triethylamine (2.14 mL) and DMF (45.0 mL) was stirred at room temperature overnight. Then, thereto was added saturated aqueous ammonium chloride solution, and then the mixture was extracted with ethyl acetate. The organic layer was washed with water and brine, and then dried over magnesium sulfate and concentrated in vacuo. The residue was purified by silica gel chromatography (eluent: chloroform/methanol=20/1) to give Compound IV (2.10 g).

Step (iv):

Compound IV (2.10 g) was dissolved in methanol (30.0 mL), and then thereto was added 10% palladium-carbon (210 mg) and the mixture was stirred under hydrogen atmosphere for 3 hours. The reaction solution was filtered, and then the filtrate was concentrated in vacuo to give the titled Compound V (1.54 g) as a white solid.

¹H-NMR (CDCl₃) δ 1.24-1.35 (m, 1H), 1.42-1.55 (m, 1H), 1.50-1.58 (m, 2H), 1.70-1.86 (m, 7H), 1.90-2.00 (m, 3H), 2.18-2.22 (m, 3H), 2.42-2.53 (m, 2H), 2.76 (s, 3H), 2.92-3.02 (m, 2H), 3.20-3.26 (m, 1H), 3.68 (s, 3H), 4.15-4.25 (m, 1H), 6.85 (d, J=8 Hz, 1H)

Compounds of Examples 140-146 were prepared in the similar manner to Example 139.

Example 140
4-Fluoro-N-[(E)-5-hydroxy-2-adamantyl-1-methyl-5-[methyl(piperidin-4-ylmethyl)amino]-1H-pyrazole-3-carboxamide

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¹H-NMR (CDCl₃) δ 1.18-1.30 (m, 2H), 1.51-1.59 (m, 2H), 1.55-1.66 (m, 1H), 1.76-1.86 (m, 8H), 1.90-1.97 (m, 2H), 2.10-2.25 (m, 3H), 2.57-2.66 (m, 2H), 2.74 (s, 3H), 2.87-2.93 (m, 2H), 3.13-3.22 (m, 2H), 3.69 (s, 3H), 4.16-4.22 (m, 1H), 6.85 (d, J=8 Hz, 1H)

Example 141
4-Fluoro-N-[(E)-5-hydroxy-2-adamantyl]-1-methyl-5-{methyl[(3R)-pyrrolidin-3-yl]amino}-1H-pyrazole-3-carboxamide

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¹H-NMR (CDCl₃) δ 1.50-1.57 (m, 2H), 1.58-1.68 (m, 1H), 1.75-1.82 (m, 7H), 1.90-2.21 (m, 2H), 2.15-2.25 (m, 3H), 2.72 (s, 3H), 2.75-2.82 (m, 1H), 2.90-3.11 (m, 3H), 3.69 (s, 3H), 3.72-3.80 (m, 1H), 4.66-4.71 (m, 1H), 6.84 (d, J=8 Hz, 1H)

Example 142
4-Fluoro-N-[(E)-5-hydroxy-2-adamantyl]-1-methyl-5-[methyl(piperidin-4-yl)amino]-1H-pyrazole-3-carboxamide

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¹H-NMR (CDCl₃) δ 1.37-1.60 (m, 6H), 1.79-1.96 (m, 10H), 2.19-2.23 (m, 3H), 2.57-2.63 (m, 2H), 2.76 (s, 3H), 3.12-3.15 (m, 2H), 3.50 (s, 3H), 3.685-3.693 (m, 1H), 4.19-4.21 (m, 1H), 6.84-6.86 (m, 1H)

Example 143
4-Fluoro-N-[(E)-5-hydroxy-2-adamantyl]-1-methyl-5-{methyl[(3S)-pyrrolidin-3-yl]amino}-1H-pyrazole-3-carboxamide

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¹H-NMR (CDCl₃) δ 1.53-1.56 (m, 13H), 2.18-2.22 (m, 3H), 2.74-2.77 (m, 4H), 2.94-3.07 (m, 4H), 3.73-3.74 (m, 3H), 3.96-3.99 (m, 1H), 4.18-4.19 (m, 1H), 7.02-7.04 (m, 1H)

Example 144
4-Fluoro-N-[(E)-5-hydroxy-2-adamantyl]-1-methyl-5-{methyl[(3R)-piperidin-3-yl]amino}-1H-pyrazole-3-carboxamide

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¹H-NMR (CDCl₃) δ 1.46-1.57 (m, 2H), 1.79-1.84 (m, 11H), 1.92-1.95 (m, 4H), 2.03-2.07 (m, 1H), 2.21 (bs, 3H), 2.74-2.81 (m, 4H), 3.28-3.31 (m, 1H), 3.48-3.51 (m, 1H), 3.74 (s, 3H), 4.16-4.17 (m, 1H), 6.89-6.91 (m, 1H)

Example 145

Benzyl 4-[[4-chloro-3-({[(E)-5-hydroxy-2-adamantyl]amino}carbonyl)-1-methyl-1H-pyrazol-5-yl]-(methyl)amino]piperidine-1-carboxylate

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¹H-NMR (CDCl₃) δ 1.50-1.60 (m, 2H), 1.68-1.97 (m, 12H), 2.15-2.25 (m, 3H), 2.75-2.90 (m, 5H), 3.19-3.30 (m, 1H), 3.70 (s, 3H), 4.05-4.27 (m, 3H), 5.12 (s, 2H), 7.01-7.03 (m, 1H), 7.28-7.40 (m, 5H)

Example 146

Benzyl 4-{[[4-chloro-3-({[(E)-5-hydroxy-2-adamantyl]amino}carbonyl)-1-methyl-1H-pyrazol-5-yl](methyl)amino]methyl}piperidine-1-carboxylate

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¹H-NMR (CDCl₃) δ 1.48-1.60 (m, 3H), 1.62-1.96 (m, 13H), 2.10-2.27 (m, 3H), 2.65-2.87 (m, 5H), 2.90-3.10 (m, 2H), 3.72 (s, 3H), 4.05-4.30 (m, 3H), 5.12 (s, 2H), 6.98-7.07 (m, 1H), 7.27-7.40 (m, 4H)

Example 147
4-Chloro-5-[[(3S)-1-(4-chlorobenzoyl)piperidin-3-yl] (methyl)amino]-N-[(E)-5-hydroxy-2-adamantyl]-1-methyl-1H-pyrazole-3-carboxamide

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Step (i):

To a solution of Compound I (20 mg) and triethylamine (20 μL) in THF (1 mL) was added 4-chlorobenzoyl chloride (10 mg), and the mixture was stirred at room temperature overnight. The reaction was quenched by methanol, and then filtered. The filtrate was concentrated in vacuo, and the residue was purified by a reverse phase HPLC (gradient condition 10%-) to give the titled Compound II (18.3 mg).

¹H-NMR (CDCl₃) δ 1.36-1.42 (m, 2H), 1.52-1.54 (m, 3H), 1.78-1.84 (m, 7H), 1.92-1.94 (m, 3H), 2.19-2.24 (m, 3H), 2.64-3.03 (m, 4H), 3.25-3.29 (m, 1H), 3.62-3.75 (m, 6H), 4.18-4.20 (m, 1H), 7.00-7.01 (m, 1H), 7.29-7.39 (m, 4H)

Example 148
4-Chloro-5-[{(3S)-1-[(3-fluorophenyl)sulfonyl] piperidin-3-yl}(methyl)amino]-N-[(E)-5-hydroxy-2-adamantyl]-1-methyl-1H-pyrazole-3-carboxamide

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To a solution of Compound I (20 mg) and triethylamine (20 μL) in THF (1 mL) was added 3-fluorobenzene sulfonyl chloride (11 mg), and the mixture was stirred at room temperature overnight. The reaction was quenched by methanol, and then filtered. The filtrate was concentrated in vacuo, and the residue was purified by a reverse phase HPLC (gradient condition 10%-) to give the titled Compound II (19.1 mg).

¹H-NMR (CDCl₃) δ 1.27-1.38 (m, 2H), 1.52-1.53 (m, 2H), 1.67-1.85 (m, 9H), 1.91-1.94 (m, 2H), 2.18-2.23 (m, 3H), 2.62-2.72 (m, 2H), 2.82 (s, 3H), 3.28-3.55 (m, 3H), 3.75 (s, 3H), 4.17-4.19 (m, 1H), 7.03-7.05 (m, 1H), 7.28-7.34 (m, 1H), 7.45-7.47 (m, 1H), 7.52-7.57 (m, 2H)

Example 149
4-[[4-Chloro-3-({[(E)-5-hydroxy-2-adamantyl]amino}carbonyl)-1-methyl-1H-pyrazol-5-yl] (methyl)amino]-N-(2-methoxyphenyl)piperidine-1-carboxamide

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To an ice-cooled solution of Compound I (20 mg) and triethylamine (20 μL) in THF (3 mL) was added 2-methoxyphenyl isocyanate (9 μL), and the mixture was stirred at room temperature for 2 hours and concentrated in vacuo. Then, the residue was purified by silica gel column chromatography (chloroform/methanol=20/1) and preparative thin-layer chromatography (ethyl acetate) to give the titled Compound II (18 mg).

¹H-NMR (CDCl₃) δ 1.17-1.60 (m, 5H), 1.66-1.96 (m, 11H), 2.08-2.20 (m, 3H), 2.75 (s, 3H), 2.83-2.91 (m, 2H), 3.20-3.28 (m, 1H), 3.65 (s, 3H), 3.81 (s, 3H), 3.94-4.20 (m, 3H), 6.80-6.95 (m, 4H), 8.00-8.10 (m, 1H)

Compounds of Examples 150-160 were prepared in the similar manner.

[Chemical Formula 104]

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Example No.
B₂

150

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151

152

153

154

155

156

157

158

159

160

Example 150

¹H-NMR (CDCl₃) δ 1.05-1.18 (m, 3H), 1.25-1.45 (m, 3H), 1.45-1.60 (m, 3H), 1.70-1.96 (m, 12H), 2.72-2.80 (m, 5H), 3.17-3.28 (m, 3H), 3.68 (s, 3H), 3.87-3.91 (s, 2H), 4.15-4.17 (m, 1H), 4.38 (s, 1H), 6.99-7.02 (m, 1H)

Example 151

¹H-NMR (CDCl₃) δ 0.86-0.91 (m, 3H), 1.27-1.58 (m, 5H), 1.60-2.08 (m, 12H), 2.10-2.30 (m, 3H), 2.72-2.85 (m, 5H), 3.10-3.28 (m, 3H), 3.68 (s, 3H), 3.87-3.91 (s, 2H), 4.15-4.18 (m, 1H), 4.46 (s, 1H), 6.99-7.02 (m, 1H)

Example 152

¹H-NMR (CDCl₃) δ 1.00-1.20 (m, 6H), 1.25-1.45 (m, 2H), 1.45-1.96 (m, 13H), 2.10-2.27 (m, 3H), 2.78-2.90 (m, 5H), 3.12-3.27 (m, 1H), 3.68 (s, 3H), 3.80-4.28 (m, 3H), 4.10-4.28 (m, 1H), 6.99-7.02 (m, 1H)

Example 153

¹H-NMR (CDCl₃) δ 1.35-1.64 (m, 5H), 1.78-1.93 (m, 10H), 2.19-2.23 (m, 3H), 2.82 (s, 3H), 2.87-3.00 (m, 2H), 3.25-3.36 (m, 1H), 3.73 (s, 3H), 4.04-4.07 (m, 2H), 4.15-4.23 (m, 1H), 6.37 (s, 1H), 6.96-7.05 (m, 3H), 7.28-7.34 (m, 2H)

Example 154

¹H-NMR (CDCl₃) δ 1.17-1.60 (m, 5H), 1.70-2.10 (m, 11H), 2.14-2.30 (m, 3H), 2.80 (s, 3H), 2.86-3.10 (m, 2H), 3.25-3.40 (m, 1H), 3.71 (s, 3H), 3.78 (s, 3H), 3.92-4.11 (m, 2H), 4.12-4.22 (m, 1H), 6.50-6.70 (m, 1H), 6.77-6.85 (m, 1H), 6.98-7.08 (m, 1H), 7.10-7.20 (m, 2H)

Example 155

¹H-NMR (CDCl₃) δ 1.40-1.58 (m, 5H), 1.72-1.95 (m, 11H), 2.14-2.27 (m, 3H), 2.82 (s, 3H), 2.88-2.94 (m, 2H), 3.25-3.35 (m, 1H), 3.72 (s, 3H), 3.78 (s, 3H), 4.03-4.06 (m, 2H), 4.15-4.22 (m, 1H), 6.83-6.85 (m, 2H), 7.02-7.04 (m, 1H), 7.22-7.24 (m, 2H)

Example 156

¹H-NMR (CDCl₃) δ 1.40-1.60 (m, 5H), 1.70-1.84 (m, 7H), 1.85-2.00 (m, 4H), 2.12-2.23 (m, 3H), 2.81 (s, 3H), 2.93-3.00 (m, 2H), 3.29-3.38 (m, 1H), 3.71 (s, 3H), 4.06-4.09 (m, 2H), 4.13-4.20 (m, 1H), 6.91-6.96 (m, 1H), 6.98-7.05 (m, 1H), 7.21-7.27 (m, 1H), 7.30-7.32 (m, 1H), 8.14-8.16 (m, 1H)

Example 157

¹H-NMR (CDCl₃) δ 1.35-1.58 (m, 5H), 1.70-1.98 (m, 10H), 2.15-2.27 (m, 3H), 2.82 (s, 3H), 2.88-2.96 (m, 2H), 3.25-3.36 (m, 1H), 3.72 (s, 3H), 4.02-4.09 (m, 2H), 4.15-4.22 (m, 1H), 6.38 (s, 1H), 7.00-7.09 (m, 1H), 7.20-7.35 (m, 4H)

Example 158

¹H-NMR (CDCl₃) δ 1.30-1.60 (m, 5H), 1.65-2.00 (m, 10H), 2.09-2.23 (m, 3H), 2.75 (s, 3H), 2.85-2.93 (m, 2H), 3.23-3.30 (m, 1H), 3.66 (s, 3H), 3.98-4.13 (m, 3H), 6.54-6.56 (m, 1H), 6.86-7.05 (m, 4H), 7.96-8.02 (m, 1H)

Example 159

¹H-NMR (CDCl₃) δ 1.34-1.69 (m, 5H), 1.70-2.05 (m, 10H), 2.11-2.28 (m, 3H), 2.80 (s, 3H), 2.88-2.96 (m, 2H), 3.25-3.38 (m, 1H), 3.70 (s, 3H), 4.01-4.20 (m, 3H), 6.43-6.49 (m, 1H), 6.68-6.75 (m, 1H), 6.95-7.03 (m, 2H), 7.15-7.35 (m, 1H)

Example 160

¹H-NMR (CDCl₃) δ 1.27-1.69 (m, 5H), 1.72-1.97 (m, 10H), 2.15-2.25 (m, 3H), 2.83 (s, 3H), 2.92-3.02 (m, 2H), 3.30-3.38 (m, 1H), 3.73 (s, 3H), 4.03-4.20 (m, 3H), 7.00-7.05 (m, 1H), 7.29-7.32 (m, 1H), 7.35-7.39 (m, 1H), 7.52-7.57 (m, 1H), 7.73-7.76 (m, 1H)

Example 161
4-[[4-Chloro-3-({[(E)-5-hydroxy-2-adamantyl]amino}carbonyl)-1-methyl-1H-pyrazol-5-yl] (methyl)amino]-N-(2,2,2-trifluoroethyl)piperidine-1-carboxamide

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To an ice-cooled solution of 1,1,1-trifluoroethylamine (4 μL) in THF (3 mL) was added chloro 4-nitrophenyl formate (10 mg), and the mixture was stirred at room temperature for 2 hours. The reaction solution was ice-cooled again, and thereto was added Compound I (20 mg) and the mixture was stirred for 2 hours. Then, thereto was added water, and the mixture was extracted with ethyl acetate. The organic layer was dried over sodium sulfate and concentrated in vacuo. The residue was purified by silica gel chromatography (chloroform/methanol=20/1) to give the titled Compound II (12 mg).

¹H-NMR (CDCl₃) δ 1.287-1.45 (m, 2H), 1.46-1.58 (m, 2H), 1.72-1.97 (m, 10H), 2.10-2.35 (m, 3H), 2.78-2.98 (m, 5H), 3.20-3.33 (m, 1H), 3.69 (s, 3H), 3.83-4.05 (m, 4H), 4.11-4.22 (m, 1H), 4.85-4.89 (m, 1H), 7.00-7.03 (m, 1H)

Compounds of Examples 162-164 were prepared in the similar manner.

[Chemical Formula 106]

embedded image

Example No.
B₂

**1

embedded image

**2

**3

Example 162

¹H-NMR (CDCl₃) δ 1.00-1.18 (m, 6H), 1.35-1.70 (m, 2H), 1.72-2.02 (m, 10H), 2.15-2.30 (m, 3H), 2.68-2.90 (m, 6H), 3.00-3.10 (m, 1H), 3.11-3.30 (m, 6H), 3.53-3.70 (m, 2H), 3.72 (s, 3H), 4.15-4.25 (m, 1H), 7.01-7.05 (m, 1H)

Example 163

¹H-NMR (CDCl₃) δ 1.40-1.63 (m, 6H), 1.78-1.97 (m, 9H), 2.15-2.27 (m, 3H), 2.83 (s, 3H), 2.90-3.03 (m, 2H), 3.27-3.38 (m, 1H), 3.73 (s, 3H), 4.09-4.18 (m, 3H), 6.66 (s, 1H), 7.04-7.06 (m, 1H), 7.22-7.25 (m, 1H), 7.96-7.99 (m, 1H), 8.25-8.30 (m, 1H), 8.41-8.46 (m, 1H)

Example 164

¹H-NMR (CDCl₃) δ 1.25-1.43 (m, 2H), 1.48-1.53 (m, 2H), 1.65-2.00 (m, 12H), 2.10-2.22 (m, 3H), 2.70-2.85 (m, 5H), 3.14-3.28 (m, 1H), 3.32 (s, 3H), 3.34-3.48 (m, 3H), 3.67 (s, 3H), 3.88-3.92 (m, 2H), 4.11-4.19 (m, 1H), 6.99-7.02 (m, 1H)

Example 165
4-Chloro-5-[[1-(5-cyanopyridin-2-yl)piperidin-4-yl] (methyl)amino]-N-[(E)-5-hydroxy-2-adamantyl]-1-methyl-1H-pyrazole-3-carboxamide

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Compound I (20.0 mg) was dissolved in DMF (1.00 mL), and then thereto were added potassium carbonate (13.0 mg) and 6-chloro-3-pyridinecarbonitrile (10.0 mg) and the mixture was stirred at 100° C. for 12 hours. Then, thereto was added water, and then the mixture was extracted with ethyl acetate. The organic layer was washed with brine, and then dried over sodium sulfate and concentrated in vacuo. The residue was purified by silica gel chromatography (eluent: chloroform/methanol=20/1) to give Compound II (11.0 mg).

¹H-NMR (CDCl₃) δ 1.34-1.54 (m, 4H), 1.72-2.00 (m, 11H), 2.10-2.30 (m, 3H), 2.79 (s, 3H), 2.90-3.07 (m, 2H), 3.31-3.48 (m, 1H), 3.68 (s, 3H), 4.13-4.17 (m, 1H), 4.36-4.40 (m, 2H), 6.56-6.59 (m, 1H), 7.00-7.03 (m, 1H), 7.55-7.58 (m, 1H), 8.30-8.45 (m, 1H)

Example 166
4-Chloro-5-[{[1-(5-cyanopyridin-2-yl)piperidin-4-yl]methyl}(methyl)amino]-N-[(E)-5-hydroxy-2-adamantyl]-1-methyl-1H-pyrazole-3-carboxamide

embedded image

¹H-NMR (CDCl₃) δ 1.07-1.36 (m, 2H), 1.34-1.60 (m, 3H), 1.70-2.07 (m, 11H), 2.10-2.35 (m, 3H), 2.77 (s, 3H), 2.85-2.93 (m, 2H), 3.00-3.03 (m, 2H), 3.71 (s, 3H), 4.15-4.17 (m, 1H), 4.38-4.43 (m, 2H), 6.55-6.58 (m, 1H), 6.99-7.01 (m, 1H), 7.53-7.57 (m, 1H), 8.30-8.39 (m, 1H)

Example 167
4-Chloro-N-[(E)-5-hydroxy-2-adamantyl]-1-methyl-5-[methyl(1-pyridin-3-ylpiperidin-4-yl)amino]-1H-pyrazole-3-carboxamide

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A solution of Compound I (50.0 mg), 3-bromopyridine (22.4 mg), sodium tertiary-butoxide (45.5 mg), 2,2′-bis(diphenylphosphino)-1,1′-binaphthyl (7.30 mg) and tris(dibenzylideneacetone)dipalladium (5.40 mg) in toluene (1.50 mL) was stirred under nitrogen at 100° C. for 3 hours. Then, thereto was added water, and then the mixture was extracted with ethyl acetate. The organic layer was washed with brine, and then dried over magnesium sulfate and concentrated in vacuo. The residue was purified by a reverse phase HPLC (gradient condition 10%-) to give the titled Compound II (28.0 mg).

¹H-NMR (CDCl₃) δ 1.50-1.68 (m, 4H), 1.76-1.86 (m, 6H), 1.90-2.00 (m, 4H), 2.16-2.25 (m, 3H), 2.75-2.85 (m, 2H), 2.84 (s, 3H), 3.22-3.32 (m, 1H), 3.62-3.70 (m, 2H), 3.73 (s, 3H), 4.16-4.22 (m, 1H), 7.02 (d, J=8 Hz, 1H), 7.12-7.19 (m, 2H), 8.08 (dd, J=2.4 Hz, 1H), 8.29 (d, J=2 Hz, 1H)

Compounds of Examples 168-180 were prepared in the similar manner to Example 167.

[Chemical Formula 110]

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Example No.
B₂

168

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169

170

171

172

173

174

175

176

177

178

179

180

181

Example 168

¹H-NMR (CDCl₃) δ 1.45-1.60 (m, 4H), 1.70-1.84 (m, 6H), 1.85-2.10 (m, 5H), 2.10-2.27 (m, 3H), 2.74-2.93 (m, 5H), 3.25-3.35 (m, 1H), 3.65-3.78 (m, 5H), 4.13-4.22 (m, 1H), 7.00-7.03 (m, 1H), 7.29 (s, 1H), 8.27 (s, 1H), 8.41 (s, 1H)

Example 169

¹H-NMR (CDCl₃) δ 1.45-1.62 (m, 4H), 1.62-1.85 (m, 7H), 1.89-2.00 (m, 4H), 2.11-2.25 (m, 3H), 2.81 (s, 3H), 2.83-2.98 (m, 2H), 3.26-3.38 (m, 1H), 3.70 (s, 3H), 3.74-3.79 (m, 2H), 4.13-4.24 (m, 1H), 7.00-7.03 (m, 1H), 7.15-7.18 (m, 1H), 7.46-7.49 (m, 1H), 8.28-8.32 (m, 1H)

Example 170

¹H-NMR (CDCl₃) δ 1.35-1.58 (m, 4H), 1.72-2.00 (m, 11H), 2.11-2.27 (m, 3H), 2.80 (s, 3H), 2.84-2.93 (m, 2H), 3.26-3.38 (m, 1H), 3.69 (s, 3H), 4.12-4.21 (m, 1H), 4.29-4.34 (m, 2H), 6.74-6.77 (m, 1H), 6.88-6.90 (m, 1H), 7.00-7.03 (m, 1H), 7.50-7.56 (m, 1H)

Example 171

¹H-NMR (CDCl₃) δ 1.35-1.70 (m, 5H), 1.70-1.97 (m, 10H), 2.11-2.25 (m, 3H), 2.72-2.90 (m, 5H), 3.20-3.34 (m, 1H), 3.65-3.80 (m, 5H), 4.13-4.21 (m, 1H), 6.89-6.92 (m, 2H), 7.01-7.03 (m, 1H), 7.43-7.46 (m, 2H)

Example 172

¹H-NMR (CDCl₃) δ 1.36-1.57 (m, 5H), 1.70-2.00 (m, 10H), 2.11-2.25 (m, 3H), 2.80 (s, 3H), 2.89-2.97 (m, 2H), 3.30-3.43 (m, 1H), 3.69 (s, 3H), 4.11-4.22 (m, 1H), 4.34-4.38 (m, 2H), 6.61-6.64 (m, 1H), 7.00-7.03 (m, 1H), 7.57-7.61 (m, 1H), 8.35 (s, 1H)

Example 173

¹H-NMR (CDCl₃) δ 1.47-1.70 (m, 5H), 1.75-1.96 (m, 10H), 2.11-2.26 (m, 3H), 2.82 (s, 3H), 2.87-2.95 (m, 2H), 3.17-3.31 (m, 1H), 3.53-3.65 (m, 2H), 4.14-4.22 (m, 1H), 7.00-7.03 (m, 1H), 6.94-7.03 (m, 2H), 7.81-7.86 (m, 1H), 8.38-8.41 (m, 1H)

Example 174

¹H-NMR (CDCl₃) δ 1.39-1.59 (m, 5H), 1.75-2.00 (m, 10H), 2.11-2.25 (m, 3H), 2.75-2.90 (m, 5H), 3.22-3.33 (m, 1H), 3.69 (s, 3H), 4.08-4.22 (m, 3H), 6.58-6.62 (m, 1H), 7.00-7.03 (m, 1H), 7.19-7.25 (m, 1H), 8.00-8.10 (m, 1H)

Example 175

¹H-NMR (CDCl₃) δ 1.49-1.69 (m, 5H), 1.75-1.93 (m, 10H), 2.11-2.25 (m, 3H), 2.68-2.75 (m, 2H), 2.82 (s, 3H), 3.00-3.10 (m, 2H), 3.11-3.25 (m, 1H), 3.74 (s, 3H), 4.13-4.21 (m, 1H), 7.02-7.04 (m, 1H), 7.16-7.21 (m, 1H), 7.28-7.31 (m, 1H), 7.45-7.50 (m, 1H), 7.57-7.60 (m, 1H)

Example 176

¹H-NMR (CDCl₃) δ 1.47-1.69 (m, 5H), 1.72-2.00 (m, 10H), 2.11-2.27 (m, 3H), 2.70-2.90 (m, 5H), 3.20-3.32 (m, 1H), 3.64-3.68 (m, 2H), 3.71 (s, 3H), 4.13-4.21 (m, 1H), 7.01-7.09 (m, 3H), 7.29-7.34 (m, 1H)

Example 177

¹H-NMR (CDCl₃) δ 1.41-1.69 (m, 5H), 1.70-1.98 (m, 10H), 2.10-2.27 (m, 3H), 2.75-2.92 (m, 5H), 3.20-3.35 (m, 1H), 3.62-3.80 (m, 5H), 4.13-4.22 (m, 1H), 6.90-6.92 (m, 2H), 7.01-7.03 (m, 1H), 7.43-7.46 (m, 2H)

Example 178

¹H-NMR (CDCl₃) δ 1.35-1.70 (m, 5H), 1.75-2.02 (m, 10H), 2.12-2.29 (m, 3H), 2.59-2.78 (m, 2H), 2.82 (s, 3H), 3.13-3.28 (m, 1H), 3.38-3.50 (m, 2H), 3.73 (s, 3H), 4.14-4.25 (m, 1H), 6.82-7.14 (m, 5H)

Example 179

¹H-NMR (CDCl₃) δ 1.39-1.69 (m, 5H), 1.70-2.02 (m, 10H), 2.12-2.27 (m, 3H), 2.68-2.90 (m, 5H), 3.17-3.30 (m, 1H), 3.57-3.68 (m, 2H), 3.71 (s, 3H), 4.13-4.25 (m, 1H), 6.50-6.66 (m, 3H), 7.00-7.03 (m, 1H), 7.11-7.19 (m, 1H)

Example 180

¹H-NMR (CDCl₃) δ 1.31-1.62 (m, 5H), 1.70-2.07 (m, 10H), 2.12-2.27 (m, 3H), 2.54-2.80 (m, 2H), 2.82 (s, 3H), 3.11-3.30 (m, 1H), 3.44-3.56 (m, 2H), 3.72 (s, 3H), 4.12-4.24 (m, 1H), 6.70-7.12 (m, 4H)

Example 181

¹H-NMR (CDCl₃) δ 1.35-1.56 (m, 5H), 1.70-1.97 (m, 10H), 2.10-2.27 (m, 3H), 2.80 (s, 3H), 2.86-2.97 (m, 2H), 3.30-3.40 (m, 1H), 3.69 (s, 3H), 4.16-4.18 (m, 1H), 4.29-4.32 (m, 2H), 6.72-6.74 (m, 1H), 6.77 (s, 1H), 7.00-7.31 (m, 1H), 8.24-8.27 (m, 1H)

The following Example Compounds, Examples A1-AX9 were prepared in the similar manner to that used in the above Examples.

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TABLE 1

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

A1

embedded image

544.4
3.56
SA

A2

embedded image

560.5
3.63
SA

A3

embedded image

556.4
3.5
SA

A4

embedded image

594.4
3.76
SA

A5

embedded image

544.5
3.6
SA

A6

embedded image

560.5
3.76
SA

A7

embedded image

556.5
3.57
SA

A8

embedded image

594.4
3.87
SA

TABLE 2

obs

Measure-

Ex.

MS
tR
ment

No.
—B
[M + 1]
(min)
Method

A9

embedded image

551.5
3.47
SA

A10

embedded image

544.6
3.58
SA

A11

embedded image

556.4
3.54
SA

A12

embedded image

551.7
3.46
SA

A13

embedded image

574.6
3.8
SA

A14

embedded image

570.4
3.56
SA

A15

embedded image

608.5
3.93
SA

A16

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558.5
3.64
SA

TABLE 3

obs

Measure-

Ex.

MS
tR
ment

No.
—B
[M + 1]
(min)
Method

A17

embedded image

570.5
3.57
SA

A18

embedded image

554.6
3.72
SA

A19

embedded image

527.6
3.1
SA

A20

embedded image

527.7
2.82
SA

A21

embedded image

527.6
2.78
SA

A22

embedded image

532.6
3.52
SA

A23

embedded image

517.5
3.27
SA

A24

embedded image

464.4
3.04
SA

TABLE 4

obs

Measure-

Ex.

MS
tR
ment

No.
—B
[M + 1]
(min)
Method

A25

embedded image

478.4
3.22
SA

A26

embedded image

492.7
3.37
SA

A27

embedded image

490.6
3.3
SA

A28

embedded image

558.5
3.66
SA

A29

embedded image

574.6
3.79
SA

A30

embedded image

558.5
3.64
SA

A31

embedded image

574.5
3.79
SA

A32

embedded image

570.4
3.63
SA

TABLE 5

obs

Measure-

Ex.

MS
tR
ment

No.
—B
[M + 1]
(min)
Method

A33

embedded image

608.5
3.9
SA

A34

embedded image

565.5
3.51
SA

A35

embedded image

608.3
3.92
SA

A36

embedded image

565.5
3.5
SA

A37

embedded image

566.4
3.75
SA

A38

embedded image

596.4
3.71
SA

A39

embedded image

584.4
3.79
SA

A40

embedded image

534.6
3.13
SA

TABLE 6

obs

Measure-

Ex.

MS
tR
ment

No.
—B
[M + 1]
(min)
Method

A41

embedded image

561.5
3.48
SA

A42

embedded image

561.5
3.28
SA

A43

embedded image

541.3
3.1
SA

A44

embedded image

557.4
3.49
SA

A45

embedded image

541.4
3.06
SA

A46

embedded image

557.3
3.37
SA

A47

embedded image

541.5
2.78
SA

TABLE 7

obs

Measure-

Ex.

MS
tR
ment

No.
—B
[M + 1]
(min)
Method

A49

embedded image

541.7
2.78
SA

A50

embedded image

541.3
2.77
SA

A51

embedded image

532.4
3.45
SA

A52

embedded image

528
3.09
SA

A53

embedded image

529.5
3.51
SA

A54

embedded image

594.4
3.9
SA

A55

embedded image

560.5
3.78
SA

TABLE 8

obs

Measure-

Ex.

MS
tR
ment

No.
—B
[M + 1]
(min)
Method

B1

embedded image

580.4
3.89
SA

B2

embedded image

596.4
3.99
SA

B3

embedded image

592.4
3.77
SA

B4

embedded image

630.5
4.1
SA

B5

embedded image

587.5
3.76
SA

B6

embedded image

580.4
3.94
SA

B7

embedded image

596.4
4.11
SA

TABLE 9

Ex.

obs MS
tR
Measurement

No.
—B
[M + 1]
(min)
Method

B9

embedded image

630.3
4.21
SA

B10

embedded image

587.5
3.82
SA

B11

embedded image

580.4
3.92
SA

B12

embedded image

596.4
4.11
SA

B13

embedded image

592.4
3.88
SA

B14

embedded image

630.5
4.23
SA

B15

embedded image

587.5
3.85
SA

B16

embedded image

563.6
3.52
SA

TABLE 10

Ex.

obs MS
tR
Measurement

No.
—B
[M + 1]
(min)
Method

B17

embedded image

594.4
3.89
SA

B18

embedded image

610.3
4.05
SA

B19

embedded image

563.6
3.47
SA

B20

embedded image

500.5
3.3
SA

B21

embedded image

514.5
3.42
SA

B22

embedded image

528.5
3.6
SA

B23

embedded image

568.5
3.77
SA

B24

embedded image

526.3
3.5
SA

TABLE 11

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

B25

embedded image

528.6
3.57
SA

TABLE 12

Ex.

obs MS
tR
Measurement

No.
—B
[M + 1]
(min)
Method

C1

embedded image

575.4
3.51
SA

C2

embedded image

493.6
3.29
SA

C3

embedded image

535.5
3.2
SA

C4

embedded image

479.5
3.03
SA

C5

embedded image

542.4
2.85
SA

C6

embedded image

542.4
2.82
SA

C7

embedded image

505.6
3.17
SA

C8

embedded image

566.5
3.47
SA

TABLE 13

Ex.

obs MS
tR
Measurement

No.
—B
[M + 1]
(min)
Method

C9

embedded image

577.5
3.62
SA

C10

embedded image

577.6
3.75
SA

C11

embedded image

577.6
3.91
SA

C12

embedded image

577.6
3.81
SA

C13

embedded image

577.5
3.49
SA

C14

embedded image

566.7
3.61
SA

C15

embedded image

560.5
3.2
SA

TABLE 14

Ex. No.
—B
obs MS [M + 1]
tR (min)
Measurement Method

C17

embedded image

569.8
3.88
SB

C18

embedded image

569.8
3.92
SB

C19

embedded image

573.7
3.8
SB

C20

embedded image

573.7
3.76
SB

C21

embedded image

589.7
3.92
SB

C22

embedded image

585.7
3.8
SB

C23

embedded image

585.7
3.72
SB

C24

embedded image

573.4
3.21
SB

TABLE 15

Ex. No.
—B
obs MS [M + 1]
tR (min)
Measurement Method

C25

embedded image

589.4
3.3
SB

C26

embedded image

589.4
3.34
SB

C27

embedded image

585.2
3.15
SB

C28

embedded image

556.3
2.51
SB

C29

embedded image

610.4
3.32
SB

C30

embedded image

572.3
2.82
SB

C31

embedded image

560.2
2.99
SB

C32

embedded image

576.2
3.13
SB

TABLE 16

Ex.

obs MS
tR
Measurement

No.
—B
[M + 1]
(min)
Method

C33

embedded image

556.3
2.53
SB

C34

embedded image

595.5
3.37
SB

C35

embedded image

569.5
3.26
SB

TABLE 17

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

D1

embedded image

524.5
4.03
SA

D2

embedded image

534.6
4.42
SA

D3

embedded image

534.6
3.97
SA

D4

embedded image

534.7
4.44
SA

D5

embedded image

517.5
3.10
SA

D6

embedded image

567.5
3.16
SA

D7

embedded image

534.5
4.40
SA

D8

embedded image

500.5
3.36
SA

TABLE 18

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

D9

embedded image

530.5
2.82
SA

D10

embedded image

499.6
2.82
SA

embedded image

TABLE 19

Ex.

obs MS
tR
Measurement

No.
—B
[M + 1]
(min)
Method

E1

embedded image

544.5
3.72
SA

E2

embedded image

528.6
3.55
SA

E3

embedded image

540.5
3.49
SA

E4

embedded image

592.5
3.89
SA

E5

embedded image

542.3
3.57
SA

E6

embedded image

558.5
3.73
SA

E7

embedded image

554.6
3.51
SA

E8

embedded image

554.6
3.5
SA

TABLE 20

Ex.

obs MS
tR
Measurement

No.
—B
[M + 1]
(min)
Method

E9

embedded image

580.5
3.68
SA

E10

embedded image

568.6
3.73
SA

E11

embedded image

584.4
3.91
SA

E12

embedded image

541.4
3.32
SA

E13

embedded image

579.6
3.59
SA

E14

embedded image

545.6
3.44
SA

E15

embedded image

579.6
3.61
SA

E16

embedded image

550.4
3.73
SA

TABLE 21

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

E17

embedded image

516.5
3.39
SA

TABLE 22

Ex.

obs MS
tR
Measurement

No.
—B
[M + 1]
(min)
Method

F1

embedded image

580.4
4.01
SA

F2

embedded image

580.4
4.02
SA

F3

embedded image

564.5
3.79
SA

F4

embedded image

564.4
3.86
SA

F5

embedded image

564.4
3.84
SA

F6

embedded image

576.6
3.97
SA

F7

embedded image

576.7
3.79
SA

F8

embedded image

614.5
4.1
SA

TABLE 23

Ex.

obs MS
tR
Measurement

No.
—B
[M + 1]
(min)
Method

F9

embedded image

614.5
4.15
SA

F10

embedded image

571.4
3.73
SA

F11

embedded image

582.4
3.89
SA

F12

embedded image

582.1
3.53
SB

F13

embedded image

582.1
3.44
SB

F14

embedded image

582.3
3.94
SA

F15

embedded image

582.6
3.79
SA

F16

embedded image

578.5
3.81
SA

TABLE 24

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

F17

embedded image

594.4
3.96
SA

F18

embedded image

552.1
3.28
SB

TABLE 25

Ex.

obs MS
tR
Measurement

No.
—B
[M + 1]
(min)
Method

G1

embedded image

559.4
3.24
SB

G2

embedded image

559.4
3.36
SB

G3

embedded image

559.4
3.34
SB

G4

embedded image

543.4
3.13
SB

G5

embedded image

543.4
3.21
SB

G6

embedded image

543.4
3.15
SB

G7

embedded image

555.2
3.19
SB

G8

embedded image

555.5
3.13
SB

TABLE 26

Ex.

obs MS
tR
Measurement

No.
—B
[M + 1]
(min)
Method

G9

embedded image

555.2
3.05
SB

G10

embedded image

561.4
3.21
SB

G11

embedded image

561.4
3.32
SB

G12

embedded image

561.4
3.24
SB

G13

embedded image

561.4
3.38
SB

G14

embedded image

561.4
3.01
SB

G15

embedded image

550.4
2.99
SB

TABLE 27

Ex.

obs MS
tR
Measurement

No.
—B
[M + 1]
(min)
Method

G17

embedded image

550.4
3.13
SB

G18

embedded image

557.4
3.11
SB

G19

embedded image

557.2
3.11
SB

G20

embedded image

557.4
3.11
SB

G21

embedded image

573.4
3.21
SB

G22

embedded image

573.4
3.26
SB

G23

embedded image

573.4
3.26
SB

G24

embedded image

553.2
3.19
SB

TABLE 28

Ex. No.
—B
obs MS [M + 1]
tR (min)
Measurement Method

G25

embedded image

553.2
3.21
SB

G26

embedded image

569.5
3.11
SB

G27

embedded image

569.8
3.67
SB

G28

embedded image

569.5
3.07
SB

G29

embedded image

540.4
2.4
SB

G30

embedded image

594.4
3.21
SB

G31

embedded image

556.3
2.71
SB

G32

embedded image

544.3
2.82
SB

TABLE 29

Ex.

obs MS
tR
Measurement

No.
—B
[M + 1]
(min)
Method

G33

embedded image

560.2
3.03
SB

G34

embedded image

540.4
2.51
SB

G35

embedded image

579.3
3.26
SB

G36

embedded image

553.2
3.17
SB

G37

embedded image

526.4
2.4
SB

TABLE 30

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

H1

embedded image

551.3
3.53
SB

H2

embedded image

508.4
3.19
SB

H3

embedded image

551.3
3.86
SB

H4

embedded image

508.4
3.49
SB

H5

embedded image

551.3
3.19
SB

H6

embedded image

508.4
3.11
SB

H7

embedded image

551.3
3.38
SB

H8

embedded image

508.4
3.26
SB

TABLE 31

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

H9

embedded image

501.4
2.71
SB

H10

embedded image

514.3
2.36
SB

H11

embedded image

484.6
2.84
SB

embedded image

TABLE 32

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

I1

embedded image

558.5
3.67
SA

I2

embedded image

574.6
3.76
SA

I3

embedded image

570.5
3.61
SA

I4

embedded image

608.5
3.87
SA

I5

embedded image

558.5
3.72
SA

I6

embedded image

574.6
3.88
SA

I7

embedded image

570.5
3.7
SA

I8

embedded image

608.5
4
SA

TABLE 33

Ex.

obs MS
tR
Measurement

No.
—B
[M + 1]
(min)
Method

I9

embedded image

565.6
3.59
SA

I10

embedded image

558.5
3.72
SA

I11

embedded image

574.6
3.91
SA

I12

embedded image

570.5
3.67
SA

I13

embedded image

608.5
4.03
SA

I14

embedded image

565.5
3.61
SA

I15

embedded image

588.4
3.93
SA

I16

embedded image

584.4
3.69
SA

TABLE 34

Measure-

Ex.

obs MS
tR
ment

No.
—B
[M + 1]
(min)
Method

I17

embedded image

622.4
4.04
SA

I18

embedded image

572.5
3.75
SA

I19

embedded image

584.5
3.71
SA

I20

embedded image

568.6
3.85
SA

I21

embedded image

541.5
3.21
SA

I22

embedded image

541.5
2.93
SA

I23

embedded image

492.5
3.35
SA

I24

embedded image

506.5
3.5
SA

TABLE 35

Ex.

obs MS
tR
Measurement

No.
—B
[M + 1]
(min)
Method

I25

embedded image

504.6
3.43
SA

I26

embedded image

572.5
3.76
SA

I27

embedded image

588.5
3.92
SA

I28

embedded image

564.4
3.6
SA

I29

embedded image

572.6
3.79
SA

I30

embedded image

588.5
3.9
SA

I31

embedded image

584.4
3.75
SA

I32

embedded image

579.6
3.62
SA

TABLE 36

Ex.

obs MS
tR
Measurement

No.
—B
[M + 1]
(min)
Method

I33

embedded image

579.6
3.61
SA

TABLE 37

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

J1

embedded image

594.5
4.02
SA

J2

embedded image

610.4
4.13
SA

J3

embedded image

606.6
3.89
SA

J4

embedded image

644.5
4.22
SA

J5

embedded image

601.5
3.89
SA

J6

embedded image

594.4
4.08
SA

J7

embedded image

610.3
4.24
SA

TABLE 38

Ex.

obs MS
tR
Measurement

No.
—B
[M + 1]
(min)
Method

J9

embedded image

644.4
4.31
SA

J10

embedded image

601.5
3.94
SA

J11

embedded image

594.4
4.05
SA

J12

embedded image

612.4
4.12
SA

J13

embedded image

606.4
4
SA

J14

embedded image

644.4
4.35
SA

J15

embedded image

601.5
3.95
SA

J16

embedded image

608.5
4.01
SA

Ex.

obs MS
tR
Measurement

No.
—B
[M + 1]
(min)
Method

J17

embedded image

624.4
4.18
SA

J18

embedded image

577.4
3.57
SA

J19

embedded image

514.6
3.42
SA

J20

embedded image

528.5
3.56
SA

J21

embedded image

542.3
3.75
SA

J22

embedded image

582.3
3.9
SA

J23

embedded image

540.6
3.65
SA

J24

embedded image

542.4
3.71
SA

TABLE 40

Ex.

obs MS
tR
Measurement

No.
—B
[M + 1]
(min)
Method

K1

embedded image

573.6
3.73
SA

K2

embedded image

573.5
3.86
SA

K3

embedded image

573.4
3.78
SA

K4

embedded image

589.5
3.93
SA

K5

embedded image

589.5
4.02
SA

K6

embedded image

585.3
3.84
SA

K7

embedded image

585.4
3.74
SA

K8

embedded image

585.4
3.65
SA

TABLE 41

Ex.

obs MS
tR
Measurement

No.
—B
[M + 1]
(min)
Method

K9

embedded image

580.4
3.73
SA

K10

embedded image

580.5
3.72
SA

K11

embedded image

493.6
3.14
SA

K12

embedded image

561.6
3.5
SA

K13

embedded image

543.6
3.39
SA

K14

embedded image

580.5
3.59
SA

K15

embedded image

591.5
3.75
SA

K16

embedded image

591.5
3.88
SA

TABLE 42

Measure-

Ex.

obs MS
tR
ment

No.
—B
[M + 1]
(min)
Method

K17

embedded image

591.4
4.01
SA

K18

embedded image

591.5
3.91
SA

K19

embedded image

591.5
3.6
SA

K20

embedded image

589.4
3.84
SA

K21

embedded image

603.4
3.38
SB

K22

embedded image

603.4
3.44
SB

K23

embedded image

603.4
3.44
SB

TABLE 43

Ex.

obs MS
tR
Measurement

No.
—B
[M + 1]
(min)
Method

K25

embedded image

624.4
3.4
SB

K26

embedded image

586.3
2.92
SB

K27

embedded image

574.3
2.99
SB

K28

embedded image

590.5
3.17
SB

K29

embedded image

570.6
2.61
SB

K30

embedded image

609.3
3.51
SB

K31

embedded image

556.3
2.53
SB

TABLE 44

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

L1

embedded image

581.3
3.78
SB

L2

embedded image

581.3
3.26
SB

L3

embedded image

581.3
3.47
SB

L4

embedded image

581.3
4.13
SB

L5

embedded image

531.1
2.82
SB

L6

embedded image

538.4
3.74
SB

L7

embedded image

538.4
3.42
SB

L8

embedded image

544.3
2.51
SB

TABLE 45

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

L9

embedded image

514.3
3.05
SB

embedded image

TABLE 46

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

M1

embedded image

542.5
3.62
SA

M2

embedded image

542.3
3.66
SA

M3

embedded image

542.5
3.66
SA

M4

embedded image

558.5
3.8
SA

M5

embedded image

558.6
3.82
SA

M6

embedded image

558.6
3.83
SA

M7

embedded image

554.6
3.56
SA

M8

embedded image

554.6
3.62
SA

TABLE 47

Ex.

obs MS
tR
Measurement

No.
—B
[M + 1]
(min)
Method

M9

embedded image

554.6
3.65
SA

M10

embedded image

592.5
3.81
SA

M11

embedded image

592.3
3.94
SA

M12

embedded image

592.3
3.96
SA

M13

embedded image

549.6
3.53
SA

M14

embedded image

549.6
3.53
SA

M15

embedded image

560.6
3.7
SA

M16

embedded image

563.7
3.59
SA

TABLE 48

Ex.

obs MS
tR
Measurement

No.
—B
[M + 1]
(min)
Method

M17

embedded image

560.5
3.74
SA

M18

embedded image

560.6
3.7
SA

M19

embedded image

606.2
3.99
SA

M20

embedded image

606.5
3.99
SA

M21

embedded image

606.3
3.99
SA

M22

embedded image

556.7
3.74
SA

M23

embedded image

556.5
3.72
SA

M24

embedded image

556.4
3.7
SA

TABLE 49

Ex.

obs MS
tR
Measurement

No.
—B
[M + 1]
(min)
Method

M25

embedded image

572.5
3.87
SA

M26

embedded image

572.5
3.85
SA

M27

embedded image

572.5
3.87
SA

M28

embedded image

563.7
3.57
SA

M29

embedded image

568.6
3.72
SA

M30

embedded image

568.6
3.64
SA

M31

embedded image

568.7
3.63
SA

M32

embedded image

462.3
3.11
SA

TABLE 50

Ex.

obs MS
tR
Measurement

No.
—B
[M + 1]
(min)
Method

M33

embedded image

476.3
3.29
SA

M34

embedded image

490.6
3.44
SA

M35

embedded image

488.6
3.37
SA

M36

embedded image

594.5
3.8
SA

M37

embedded image

582.3
3.88
SA

M38

embedded image

598.7
4.04
SA

M39

embedded image

539.7
2.82
SA

M40

embedded image

555.6
3.43
SA

TABLE 51

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

M41

embedded image

593.6
3.7
SA

M42

embedded image

559.5
3.56
SA

M43

embedded image

593.5
3.71
SA

M44

embedded image

564.5
3.94
SA

M45

embedded image

530.5
3.51
SA

TABLE 52

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

N1

embedded image

594.5
4.04
SA

N2

embedded image

594.4
4.16
SA

N3

embedded image

594.4
4.16
SA

N4

embedded image

578.3
3.93
SA

N5

embedded image

578.5
4.01
SA

N6

embedded image

578.5
3.97
SA

N7

embedded image

590.4
3.71
SA

TABLE 53

Ex.

obs MS

Measurement

No.
—B
[M + 1]
tR (min)
Method

N9

embedded image

590.5
3.84
SA

N10

embedded image

628.5
4.13
SA

N11

embedded image

628.6
4.24
SA

N12

embedded image

628.5
4.26
SA

N13

embedded image

585.6
3.81
SA

N14

embedded image

585.4
3.86
SA

N15

embedded image

585.4
3.9
SA

N16

embedded image

596.3
4.02
SA

TABLE 54

Ex.

obs MS

Measurement

No.
—B
[M + 1]
tR (min)
Method

N17

embedded image

596.4
4.12
SA

N18

embedded image

596.4
4.03
SA

N19

embedded image

596.4
4.11
SA

N20

embedded image

596.4
3.93
SA

N21

embedded image

592.3
3.93
SA

N22

embedded image

608.5
4.09
SA

N23

embedded image

566.4
3.8
SA

TABLE 55

Ex.

obs MS

Measurement

No.
—B
[M + 1]
tR (min)
Method

O1

embedded image

557.7
3.8
SB

O2

embedded image

557.7
3.92
SB

O3

embedded image

557.4
3.88
SB

O4

embedded image

573.7
3.97
SB

O5

embedded image

573.7
4.03
SB

O6

embedded image

573.7
4.03
SB

O7

embedded image

569.8
3.9
SB

O8

embedded image

569.8
3.78
SB

TABLE 56

Measure-

Ex.

obs MS

ment

No.
—B
[M + 1]
tR (min)
Method

O9

embedded image

569.8
3.74
SB

O10

embedded image

564.7
3.67
SB

O11

embedded image

564.7
3.8
SB

O12

embedded image

564.7
3.8
SB

O13

embedded image

575.7
3.82
SB

O14

embedded image

575.7
3.95
SB

O15

embedded image

575.7
3.69
SB

O16

embedded image

575.7
3.99
SB

TABLE 57

Measure-

Ex.

obs MS
tR
ment

No.
—B
[M + 1]
(min)
Method

O17

embedded image

575.7
4.05
SB

O18

embedded image

587.4
3.32
SB

O19

embedded image

587.4
3.4
SB

O20

embedded image

587.4
3.36
SB

O21

embedded image

557.4
3.34
SB

O22

embedded image

593.6
3.38
SB

O23

embedded image

540.4
2.48
SB

TABLE 58

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

P1

embedded image

565.6
3.67
SB

P2

embedded image

565.6
3.15
SB

P3

embedded image

565.6
3.36
SB

P4

embedded image

565.6
4.07
SB

P5

embedded image

515.4
2.71
SB

P6

embedded image

522.2
3.63
SB

P7

embedded image

522.2
3.40
SB

P8

embedded image

528.3
2.44
SB

TABLE 59

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

P9

embedded image

498.3
2.94
SB

P10

embedded image

522.4
3.36
SB

embedded image

TABLE 60

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

Q1

embedded image

572.6
3.87
SA

Q2

embedded image

588.6
4.11
SA

Q3

embedded image

584.5
3.8
SA

Q4

embedded image

572.6
3.9
SA

Q5

embedded image

588.6
4.07
SA

Q6

embedded image

584.5
3.85
SA

Q7

embedded image

572.5
3.88
SA

Q8

embedded image

588.6
4.06
SA

TABLE 61

Measure-

Ex.

obs MS

ment

No.
—B
[M + 1]
tR (min)
Method

Q9

embedded image

584.5
3.82
SA

Q10

embedded image

602.6
4.08
SA

Q11

embedded image

598.6
3.83
SA

Q12

embedded image

586.6
3.91
SA

Q13

embedded image

598.7
3.84
SA

Q14

embedded image

555.5
3.35
SA

Q15

embedded image

555.5
3.06
SA

Q16

embedded image

555.5
3
SA

TABLE 62

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

Q17

embedded image

492.7
3.34
SA

Q18

embedded image

506.7
3.52
SA

Q19

embedded image

520.7
3.68
SA

Q20

embedded image

518.7
3.61
SA

TABLE 63

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

R1

embedded image

608.3
4.17
SA

R2

embedded image

620.4
4.04
SA

R3

embedded image

608.4
4.23
SA

R4

embedded image

624.4
4.4
SA

R5

embedded image

620.5
4.19
SA

R6

embedded image

608.3
4.21
SA

R7

embedded image

624.5
4.41
SA

R8

embedded image

622.6
4.15
SA

TABLE 64

Ex.

obs MS

Measurement

No.
—B
[M + 1]
tR (min)
Method

R9

embedded image

638.4
4.31
SA

R10

embedded image

591.4
3.72
SA

R11

embedded image

528.6
3.61
SA

R12

embedded image

542.5
3.74
SA

R13

embedded image

556.5
3.94
SA

R14

embedded image

554.4
3.77
SA

R15

embedded image

624.4
4.29
SA

R16

embedded image

620.5
4.15
SA

TABLE 65

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

R17

embedded image

556.5
3.83
SA

TABLE 66

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

S1

embedded image

552.4
3.74
SB

S2

embedded image

595.5
3.97
SB

S3

embedded image

595.5
3.69
SB

S4

embedded image

545.4
2.90
SB

S5

embedded image

552.4
3.34
SB

S6

embedded image

552.4
3.57
SB

S7

embedded image

558.3
2.61
SB

embedded image

TABLE 67

Ex.

obs MS

Measurement

No.
—B
[M + 1]
tR (min)
Method

U1

embedded image

530.5
3.43
SA

U2

embedded image

546.5
3.56
SA

U3

embedded image

542.5
3.39
SA

U4

embedded image

580.4
3.72
SA

U5

embedded image

537.6
3.31
SA

U6

embedded image

530.5
3.41
SA

U7

embedded image

546.6
3.58
SA

U8

embedded image

542.6
3.37
SA

TABLE 68

Ex.

obs MS
tR
Measurement

No.
—B
[M + 1]
(min)
Method

U9

embedded image

580.4
3.73
SA

U10

embedded image

537.6
3.31
SA

U11

embedded image

560.6
3.64
SA

U12

embedded image

556.7
3.46
SA

U13

embedded image

594.5
3.82
SA

U14

embedded image

544.6
3.48
SA

U15

embedded image

556.7
3.46
SA

U16

embedded image

540.6
3.6
SA

TABLE 69

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

U17

embedded image

513.7
3.07
SA

U18

embedded image

513.7
2.73
SA

U19

embedded image

513.7
2.68
SA

U20

embedded image

518.6
3.35
SA

U21

embedded image

450.5
2.92
SA

U22

embedded image

464.5
3.08
SA

U23

embedded image

478.5
3.21
SA

U24

embedded image

476.3
3.16
SA

TABLE 70

Ex.

obs MS
tR
Measurement

No.
—B
[M + 1]
(min)
Method

U25

embedded image

518.6
3.3
SA

U26

embedded image

530.5
3.42
SA

U27

embedded image

582.5
3.62
SA

U28

embedded image

570.6
3.68
SA

U29

embedded image

586.5
3.91
SA

U30

embedded image

527.7
2.7
SA

U31

embedded image

543.7
3.24
SA

U32

embedded image

581.5
3.51
SA

TABLE 71

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

U33

embedded image

547.7
3.49
SA

U34

embedded image

581.5
3.65
SA

U35

embedded image

552.4
3.73
SA

U36

embedded image

518.7
3.33
SA

TABLE 72

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

V1

embedded image

566.4
3.72
SA

V2

embedded image

582.3
3.85
SA

V3

embedded image

578.4
3.61
SA

V4

embedded image

573.6
3.62
SA

V5

embedded image

566.5
3.78
SA

V6

embedded image

582.1
3.92
SA

V7

embedded image

578.5
3.74
SA

TABLE 73

Ex.

obs MS
tR
Measurement

No.
—B
[M + 1]
(min)
Method

V9

embedded image

566.4
3.76
SA

V10

embedded image

582.3
3.94
SA

V11

embedded image

578.5
3.7
SA

V12

embedded image

573.5
3.68
SA

V13

embedded image

580.4
3.66
SA

V14

embedded image

596.3
3.9
SA

V15

embedded image

549.5
3.31
SA

V16

embedded image

486.5
3.13
SA

TABLE 74

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

V17

embedded image

514.5
3.45
SA

V18

embedded image

554.5
3.64
SA

V19

embedded image

512.4
3.33
SA

V20

embedded image

514.5
3.41
SA

V21

embedded image

584.4
3.89
SA

V22

embedded image

584.4
3.87
SA

V23

embedded image

584.4
3.72
SA

TABLE 75

Ex.

obs MS
tR
Measurement

No.
—B
[M + 1]
(min)
Method

W1

embedded image

561.7
3.84
SB

W2

embedded image

561.7
3.9
SB

W3

embedded image

561.7
3.86
SB

W4

embedded image

545.7
3.65
SB

W5

embedded image

545.7
3.76
SB

W6

embedded image

545.4
3.18
SB

W7

embedded image

557.4
3.17
SB

W8

embedded image

557.4
3.07
SB

TABLE 76

Ex.

obs MS
tR
Measurement

No.
—B
[M + 1]
(min)
Method

W9

embedded image

557.4
3.01
SB

W10

embedded image

563.3
3.11
SB

W11

embedded image

563.3
3.26
SB

W12

embedded image

563.3
3.19
SB

W13

embedded image

563.3
2.96
SB

W14

embedded image

552.1
2.96
SB

W15

embedded image

552.1
3.19
SB

W16

embedded image

552.1
3.11
SB

TABLE 77

Measure-

Ex.

obs MS
tR
ment

No.
—B
[M + 1]
(min)
Method

W17

embedded image

559.4
3.09
SB

W18

embedded image

559.4
3.07
SB

W19

embedded image

559.4
3.15
SB

W20

embedded image

575.4
3.18
SB

W21

embedded image

575.4
3.26
SB

W22

embedded image

575.4
3.24
SB

W23

embedded image

555.2
3.17
SB

W24

embedded image

555.2
3.19
SB

TABLE 78

Mea-

sure-

Ex.

obs MS
tR
ment

No.
—B
[M + 1]
(min)
Method

W25

embedded image

571.5
3.11
SB

W26

embedded image

571.5
3.07
SB

W27

embedded image

571.5
3.03
SB

W28

embedded image

542.3
2.38
SB

W29

embedded image

596.4
3.17
SB

W30

embedded image

558.3
2.69
SB

W31

embedded image

546.2
2.8
SB

W32

embedded image

562.2
2.99
SB

TABLE 79

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

W33

embedded image

563.3
3.34
SB

W34

embedded image

581.3
3.24
SB

W35

embedded image

555.2
3.13
SB

W37

embedded image

528.3
2.38
SB

TABLE 80

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

X1

embedded image

553.2
3.63
SB

X2

embedded image

553.2
2.84
SB

X3

embedded image

553.2
2.99
SB

X4

embedded image

553.2
3.86
SB

X5

embedded image

503.4
2.44
SB

X6

embedded image

510.4
3.42
SB

X7

embedded image

510.4
2.78
SB

X8

embedded image

510.4
3.19
SB

TABLE 81

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

X9

embedded image

516.6
2.34
SB

X10

embedded image

486.6
2.71
SB

X11

embedded image

510.4
3.05
SB

embedded image

TABLE 82

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

Y1

embedded image

530.5
3.4
SA

Y2

embedded image

530.5
3.42
SA

Y3

embedded image

546.6
3.58
SA

Y4

embedded image

542.5
3.38
SA

Y5

embedded image

537.5
3.31
SA

Y6

embedded image

530.5
3.41
SA

Y7

embedded image

546.7
3.58
SA

Y8

embedded image

580.4
3.72
SA

TABLE 83

obs

Measure-

Ex.

MS
tR
ment

No.
—B
[M + 1]
(min)
Method

Y9

embedded image

537.5
3.32
SA

Y10

embedded image

560.7
3.67
SA

Y11

embedded image

556.4
3.45
SA

Y12

embedded image

580.4
3.7
SA

Y13

embedded image

542.6
3.37
SA

Y14

embedded image

556.5
3.47
SA

Y15

embedded image

594.5
3.82
SA

Y16

embedded image

544.6
3.5
SA

TABLE 84

obs

Measure-

Ex.

MS
tR
ment

No.
—B
[M + 1]
(min)
Method

Y17

embedded image

476.5
3.16
SA

Y18

embedded image

582.5
3.6
SA

Y19

embedded image

549.6
3.49
SA

Y20

embedded image

548.5
3.53
SA

Y21

embedded image

548.6
3.54
SA

Y22

embedded image

594.4
3.78
SA

Y23

embedded image

594.4
3.82
SA

TABLE 85

obs

Measure-

Ex.

MS
tR
ment

No.
—B
[M + 1]
(min)
Method

Y25

embedded image

544.5
3.59
SA

Y26

embedded image

560.6
3.64
SA

Y27

embedded image

560.6
3.67
SA

Y28

embedded image

551.7
3.39
SA

Y29

embedded image

551.5
3.38
SA

Y30

embedded image

556.5
3.52
SA

Y31

embedded image

570.4
3.64
SA

Y32

embedded image

527.7
2.69
SA

TABLE 86

obs

Measure-

Ex.

MS
tR
ment

No.
—B
[M + 1]
(min)
Method

Y33

embedded image

543.6
3.2
SA

Y34

embedded image

581.5
3.48

Y35

embedded image

547.6
3.45

Y36

embedded image

581.5
3.64

Y37

embedded image

552.4
3.72

Y38

embedded image

518.6
3.32

TABLE 87

obs

Measure-

Ex.

MS
tR
ment

No.
—B
[M + 1]
(min)
Method

Z1

embedded image

566.5
3.72
SA

Z2

embedded image

582.3
3.85
SA

Z3

embedded image

578.3
3.62
SA

Z4

embedded image

573.5
3.63
SA

Z5

embedded image

566.5
3.79
SA

Z6

embedded image

582.4
3.93
SA

Z7

embedded image

578.5
3.76
SA

TABLE 88

obs

Measure-

Ex.

MS
tR
ment

No.
—B
[M + 1]
(min)
Method

Z9

embedded image

566.4
3.76
SA

Z10

embedded image

582.3
3.94
SA

Z11

embedded image

578.5
3.7
SA

Z12

embedded image

573.6
3.69
SA

Z13

embedded image

580.4
3.75
SA

Z14

embedded image

596.4
3.9
SA

Z15

embedded image

549.5
3.3
SA

Z16

embedded image

486.5
3.13
SA

TABLE 89

obs

Measure-

Ex.

MS
tR
ment

No.
—B
[M + 1]
(min)
Method

Z17

embedded image

500.5
3.27
SA

Z18

embedded image

514.5
3.46
SA

Z19

embedded image

554.5
3.64
SA

Z20

embedded image

512.5
3.34
SA

Z21

embedded image

514.5
3.43
SA

Z22

embedded image

584.4
3.9
SA

Z23

embedded image

584.4
3.88
SA

Z24

embedded image

584.4
3.71
SA

TABLE 90

obs

Measure-

Ex.

MS
tR
ment

No.
—B
[M + 1]
(min)
Method

Z25

embedded image

584.4
3.82
SA

TABLE 91

obs

Measure-

Ex.

MS
tR
ment

No.
—B
[M + 1]
(min)
Method

AA1

embedded image

561.6
3.74
SA

AA2

embedded image

557.2
3.17
SB

AA3

embedded image

552.1
2.96
SB

AA4

embedded image

552.1
3.09
SB

AA5

embedded image

552.1
3.09
SB

AA6

embedded image

563.6
3.51
SA

AA7

embedded image

563.3
3.32
SB

AA8

embedded image

563.3
2.96
SB

TABLE 92

obs

Measure-

Ex.

MS
tR
ment

No.
—B
[M + 1]
(min)
Method

AA9

embedded image

563.6
3.77
SA

AA10

embedded image

563.3
3.26
SB

AA11

embedded image

555.2
3.17
SB

AA12

embedded image

559.4
3.09
SB

AA13

embedded image

575.4
3.21
SB

AA14

embedded image

571.5
3.09
SB

AA15

embedded image

571.5
3.09
SB

AA16

embedded image

559.4
3.09
SB

TABLE 93

obs

Measure-

Ex.

MS
tR
ment

No.
—B
[M + 1]
(min)
Method

AA17

embedded image

575.4
3.17
SB

AA18

embedded image

575.4
3.21
SB

AA19

embedded image

571.5
3.03
SB

AA20

embedded image

542.3
2.36
SB

AA21

embedded image

596.4
3.17
SB

AA22

embedded image

558.3
2.71
SB

TABLE 94

obs

Measure-

Ex.

MS
tR
ment

No.
—B
[M + 1]
(min)
Method

AB1

embedded image

553.2
3.59
SB

AB2

embedded image

553.2
2.86
SB

AB3

embedded image

553.2
2.99
SB

AB4

embedded image

553.2
3.84
SB

AB5

embedded image

503.4
2.44
SB

AB6

embedded image

516.3
2.34
SB

AB7

embedded image

486.3
2.69
SB

AB8

embedded image

510.4
3.05
SB

embedded image

TABLE 95

obs

Measure-

Ex.

MS
tR
ment

No.
—B
[M + 1]
(min)
Method

AC1

embedded image

514.5
3.47
SA

AC2

embedded image

540.6
3.38
SA

AC3

embedded image

530.5
3.52
SA

AC4

embedded image

526.7
3.32
SA

AC5

embedded image

564.6
3.65
SA

AC6

embedded image

521.7
3.24
SA

AC7

embedded image

514.5
3.34
SA

AC8

embedded image

530.5
3.51
SA

TABLE 96

obs

Measure-

Ex.

MS
tR
ment

No.
—B
[M + 1]
(min)
Method

AC9

embedded image

526.7
3.3
SA

AC10

embedded image

564.5
3.66
SA

AC11

embedded image

521.7
3.24
SA

AC12

embedded image

544.5
3.59
SA

AC13

embedded image

540.6
3.34
SA

AC14

embedded image

578.5
3.74
SA

AC15

embedded image

528.6
3.43
SA

AC16

embedded image

434.6
2.85
SA

TABLE 97

Measure-

Ex.

obs MS
tR
ment

No.
—B
[M + 1]
(min)
Method

AC17

embedded image

448.6
3
SA

AC18

embedded image

462.5
3.13
SA

AC19

embedded image

460.5
3.09
SA

AC20

embedded image

514.6
3.33
SA

AC21

embedded image

532.6
3.47
SA

AC22

embedded image

532.6
3.45
SA

AC23

embedded image

532.5
3.41
SA

AC24

embedded image

578.5
3.67
SA

TABLE 98

Measure-

Ex.

obs MS
tR
ment

No.
—B
[M + 1]
(min)
Method

AC25

embedded image

578.5
3.74
SA

AC26

embedded image

528.6
3.46
SA

AC27

embedded image

528.5
3.45
SA

AC28

embedded image

544.5
3.54
SA

AC29

embedded image

544.5
3.58
SA

AC30

embedded image

535.7
3.31
SA

AC31

embedded image

535.6
3.3
SA

AC32

embedded image

540.4
3.45
SA

TABLE 99

Measure-

Ex.

obs MS
tR
ment

No.
—B
[M + 1]
(min)
Method

AC33

embedded image

566.4
3.49
SA

AC34

embedded image

554.4
3.55
SA

AC35

embedded image

570.5
3.73
SA

AC36

embedded image

511.5
2.62
SA

AC37

embedded image

527.3
3.16
SA

AC38

embedded image

565.4
3.42
SA

AC39

embedded image

531.4
3.35
SA

AC40

embedded image

565.4
3.61
SA

TABLE 100

Measure-

Ex.

obs MS
tR
ment

No.
—B
[M + 1]
(min)
Method

AC41

embedded image

536.5
3.62
SA

AC42

embedded image

502.5
3.23
SA

TABLE 101

Measure-

Ex.

obs MS
tR
ment

No.
—B
[M + 1]
(min)
Method

AD1

embedded image

550.4
3.66
SA

AD2

embedded image

566.4
3.83
SA

AD3

embedded image

562.6
3.55
SA

AD4

embedded image

557.5
3.55
SA

AD5

embedded image

550.6
3.71
SA

AD6

embedded image

566.5
3.85
SA

AD7

embedded image

562.5
3.67
SA

TABLE 102

Measure-

Ex.

obs MS
tR
ment

No.
—B
[M + 1]
(min)
Method

AD9

embedded image

550.5
3.68
SA

AD10

embedded image

566.5
3.85
SA

AD11

embedded image

562.6
3.63
SA

AD12

embedded image

557.5
3.61
SA

AD13

embedded image

564.5
3.67
SA

AD14

embedded image

580.5
3.84
SA

AD15

embedded image

533.7
3.34
SA

AD16

embedded image

470.5
3.05
SA

TABLE 103

Measure-

Ex.

obs MS
tR
ment

No.
—B
[M + 1]
(min)
Method

AD17

embedded image

484.5
3.21
SA

AD18

embedded image

538.7
3.55
SA

AD19

embedded image

568.6
3.84
SA

AD20

embedded image

568.5
3.8
SA

AD21

embedded image

568.5
3.63
SA

AD22

embedded image

568.6
3.73
SA

AD23

embedded image

600.6
3.87
SA

TABLE 104

Measure-

Ex.

obs MS
tR
ment

No.
—B
[M + 1]
(min)
Method

AD25

embedded image

600.5
4
SA

AD26

embedded image

568.6
3.75
SA

TABLE 105

Measure-

Ex.

obs MS
tR
ment

No.
—B
[M + 1]
(min)
Method

AE1

embedded image

529.4
2.99
SB

AE2

embedded image

529.2
3.11
SB

AE3

embedded image

529.2
3.03
SB

AE4

embedded image

545.4
3.15
SB

AE5

embedded image

545.4
3.24
SB

AE6

embedded image

545.4
3.21
SB

AE7

embedded image

541.5
3.09
SB

AE8

embedded image

541.5
3.01
SB

TABLE 106

Measure-

Ex.

obs MS
tR
ment

No.
—B
[M + 1]
(min)
Method

AE9

embedded image

536.4
2.88
SB

AE10

embedded image

536.4
3.03
SB

AE11

embedded image

536.4
3.01
SB

AE12

embedded image

536.4
3.01
SB

AE13

embedded image

547.6
3.69
SB

AE14

embedded image

547.6
3.47
SB

AE15

embedded image

547.6
3.76
SB

TABLE 107

Measure-

Ex.

obs MS
tR
ment

No.
—B
[M + 1]
(min)
Method

AE17

embedded image

539.8
3.69
SB

AE18

embedded image

539.8
3.69
SB

AE19

embedded image

543.7
3.63
SB

AE20

embedded image

543.7
3.67
SB

AE21

embedded image

559.7
4.01
SB

AE22

embedded image

555.8
3.61
SB

AE23

embedded image

555.8
3.57
SB

AE24

embedded image

543.4
3.01
SB

TABLE 108

Measure-

Ex.

obs MS
tR
ment

No.
—B
[M + 1]
(min)
Method

AE25

embedded image

559.4
3.11
SB

AE26

embedded image

559.4
3.15
SB

AE27

embedded image

555.2
2.94
SB

AE28

embedded image

565.6
3.14
SB

AE29

embedded image

539.5
3.07
SB

AE30

embedded image

512.4
2.3
SB

AE31

embedded image

526.4
2.32
SB

AE32

embedded image

580.4
3.11
SB

TABLE 109

Measure-

Ex.

obs MS
tR
ment

No.
—B
[M + 1]
(min)
Method

AE33

embedded image

542.6
2.63
SB

AE34

embedded image

546.2
2.9
SB

TABLE 110

Measure-

Ex.

obs MS
tR
ment

No.
—B
[M + 1]
(min)
Method

AF1

embedded image

537.6
3.47
SB

AF2

embedded image

537.6
2.73
SB

AF3

embedded image

537.6
2.94
SB

AF4

embedded image

537.6
3.74
SB

AF5

embedded image

487.4
2.38
SB

AF6

embedded image

494.4
2.67
SB

AF7

embedded image

494.4
3.09
SB

AF8

embedded image

500.3
2.30
SB

TABLE 111

Measure-

Ex.

obs MS
tR
ment

No.
—B
[M + 1]
(min)
Method

AF9

embedded image

470.3
2.61
SB

AF10

embedded image

494.4
2.94
SB

embedded image

TABLE 112

Ex.

obs MS
tR
Measurement

No.
—B
[M + 1]
(min)
Method

AG1

embedded image

529.4
3.05
SB

AG2

embedded image

529.4
3.13
SB

AG3

embedded image

529.4
3.05
SB

AG4

embedded image

545.4
3.15
SB

AG5

embedded image

545.4
3.24
SB

AG6

embedded image

545.4
3.21
SB

AG7

embedded image

541.5
3.07
SB

AG8

embedded image

541.5
3.01
SB

TABLE 113

Ex.

obs MS
tR
Measurement

No.
—B
[M + 1]
(min)
Method

AG9

embedded image

541.5
2.94
SB

AG10

embedded image

536.4
2.9
SB

AG11

embedded image

536.4
3.03
SB

AG12

embedded image

536.4
3.03
SB

AG13

embedded image

547.4
3.01
SB

AG14

embedded image

547.4
3.11
SB

AG15

embedded image

547.4
2.88
SB

AG16

embedded image

547.4
3.17
SB

TABLE 114

Meas-

obs MS
tR
urement

Ex. No.
—B
[M + 1]
(min)
Method

AG17

embedded image

547.4
3.26
SB

AG18

embedded image

539.5
3.11
SB

AG19

embedded image

539.5
3.09
SB

AG20

embedded image

543.4
3.01
SB

AG21

embedded image

543.4
3.01
SB

AG22

embedded image

559.4
3.15
SB

AG23

embedded image

555.2
3.03
SB

AG24

embedded image

555.2
2.96
SB

TABLE 115

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

AH1

embedded image

537.6
3.47
SB

AH2

embedded image

537.6
2.73
SB

AH3

embedded image

537.6
2.88
SB

AH4

embedded image

537.6
3.74
SB

AH5

embedded image

487.4
2.42
SB

AH6

embedded image

494.4
3.34
SB

AH7

embedded image

494.4
2.67
SB

AH8

embedded image

494.4
3.09
SB

TABLE 116

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

AH9

embedded image

500.3
2.28
SB

AH10

embedded image

470.3
2.61
SB

AH11

embedded image

494.4
2.94
SB

embedded image

TABLE 117

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

AI1

embedded image

560.6
3.64
SA

AI2

embedded image

560.5
3.72
SA

AI3

embedded image

560.5
3.72
SA

AI4

embedded image

544.6
3.6
SA

AI5

embedded image

544.6
3.6
SA

AI6

embedded image

544.5
3.58
SA

AI7

embedded image

556.5
3.7
SA

AI8

embedded image

556.5
3.57
SA

TABLE 118

Meas-

obs MS
tR
urement

Ex. No.
—B
[M + 1]
(min)
Method

AI9

embedded image

556.5
3.56
SA

AI10

embedded image

594.5
3.78
SA

AI11

embedded image

594.4
3.89
SA

AI12

embedded image

594.4
3.91
SA

AI13

embedded image

562.5
3.66
SA

AI14

embedded image

562.6
3.68
SA

AI15

embedded image

562.5
3.67
SA

AI16

embedded image

608.5
3.98
SA

TABLE 119

Ex.

obs MS
tR
Measurement

No.
—B
[M + 1]
(min)
Method

AI17

embedded image

558.6
3.68
SA

AI18

embedded image

574.5
3.79
SA

AI19

embedded image

570.6
3.63
SA

AI20

embedded image

570.6
3.58
SA

AI21

embedded image

464.4
3.13
SA

AI22

embedded image

478.5
3.31
SA

AI23

embedded image

492.6
3.42
SA

AI24

embedded image

596.5
3.7
SA

TABLE 120

Ex.

obs MS
tR
Measurement

No.
—B
[M + 1]
(min)
Method

AI25

embedded image

584.6
3.8
SA

AI26

embedded image

600.5
3.96
SA

AI27

embedded image

541.6
2.84
SA

AI28

embedded image

557.6
3.4
SA

AI29

embedded image

595.5
3.66
SA

AI30

embedded image

561.7
3.52
SA

AI31

embedded image

595.4
3.71
SA

AI32

embedded image

566.5
4
SA

TABLE 121

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

AI33

embedded image

532.6
3.54
SA

AI34

embedded image

532.6
3.54
SA

TABLE 122

Meas-

obs MS

urement

Ex. No.
—B
[M + 1]
tR (min)
Method

AJ1

embedded image

596.4
4.01
SA

AJ2

embedded image

596.4
4.12
SA

AJ3

embedded image

596.5
4.14
SA

AJ4

embedded image

580.4
3.93
SA

AJ5

embedded image

580.4
3.97
SA

AJ6

embedded image

592.4
3.83
SA

AJ7

embedded image

592.3
3.97
SA

AJ8

embedded image

592.5
3.93
SA

TABLE 123

Meas-

obs MS

urement

Ex. No.
—B
[M + 1]
tR (min)
Method

AJ9

embedded image

630.5
4.11
SA

AJ10

embedded image

630.5
4.21
SA

AJ11

embedded image

630.5
4.27
SA

AJ12

embedded image

598.6
4.02
SA

AJ13

embedded image

598.6
4.06
SA

AJ14

embedded image

598.5
4
SA

AJ15

embedded image

598.6
4.09
SA

AJ16

embedded image

598.6
3.91
SA

TABLE 124

Meas-

obs MS

urement

Ex. No.
—B
[M + 1]
tR (min)
Method

AJ17

embedded image

594.4
3.89
SA

AJ18

embedded image

610.3
4.05
SA

AJ19

embedded image

500.5
3.33
SA

AJ20

embedded image

514.5
3.47
SA

AJ21

embedded image

528.5
3.58
SA

AJ22

embedded image

526.5
3.54
SA

AJ23

embedded image

568.3
3.76
SA

AJ24

embedded image

563.6
3.46
SA

TABLE 125

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

AJ25

embedded image

528.6
3.67
SA

TABLE 126

Meas-

obs MS
tR
urement

Ex. No.
—B
[M + 1]
(min)
Method

AK1

embedded image

559.7
3.78
SB

AK2

embedded image

559.7
3.86
SB

AK3

embedded image

575.7
3.95
SB

AK4

embedded image

575.7
0.69
SB

AK5

embedded image

575.4
3.38
SB

AK6

embedded image

571.7
3.9
SB

AK7

embedded image

571.7
3.78
SB

AK8

embedded image

571.7
3.72
SB

TABLE 127

obs

Measure-

Ex.

MS
tR
ment

No.
—B
[M + 1]
(min)
Method

AK9

embedded image

566.7
3.65
SB

AK10

embedded image

566.7
3.8
SB

AK11

embedded image

566.7
3.76
SB

AK12

embedded image

577.9
3.8
SB

AK13

embedded image

577.9
3.84
SB

AK14

embedded image

577.9
0.65
SB

AK15

embedded image

577.9
0.65
SB

TABLE 128

obs

Measure-

Ex.

MS
tR
ment

No.
—B
[M + 1]
(min)
Method

AK17

embedded image

569.5
3.32
SB

AK18

embedded image

569.5
3.3
SB

AK19

embedded image

573.4
3.19
SB

AK20

embedded image

573.4
3.21
SB

AK21

embedded image

589.4
3.36
SB

AK22

embedded image

585.2
3.21
SB

AK23

embedded image

585.2
3.17
SB

AK24

embedded image

573.4
3.24
SB

TABLE 129

Measurement

Ex. No.
—B
obs MS [M + 1]
tR (min)
Method

AK25

embedded image

589.4
3.32
SB

AK26

embedded image

589.4
3.36
SB

AK27

embedded image

585.2
3.17
SB

AK28

embedded image

556.3
2.44
SB

AK29

embedded image

610.4
3.3
SB

AK30

embedded image

572.3
2.82
SB

AK31

embedded image

560.2
2.9
SB

AK32

embedded image

576.2
3.11
SB

TABLE 130

obs

Measure-

Ex.

MS
tR
ment

No.
—B
[M + 1]
(min)
Method

AK33

embedded image

556.3
2.51
SB

AK34

embedded image

595.3
3.35
SB

AK35

embedded image

542.3
2.44
SB

TABLE 131

obs

Measure-

Ex.

MS
tR
ment

No.
—B
[M + 1]
(min)
Method

AL1

embedded image

567.3
3.69
SB

AL2

embedded image

567.3
3.42
SB

AL3

embedded image

567.3
3.65
SB

AL4

embedded image

567.5
3.97
SB

AL5

embedded image

517.4
2.94
SB

AL6

embedded image

524.4
3.57
SB

AL7

embedded image

524.4
3.32
SB

AL8

embedded image

524.4
3.28
SB

TABLE 132

obs

Measure-

Ex.

MS
tR
ment

No.
—B
[M + 1]
(min)
Method

AL9

embedded image

530.3
2.40
SB

AL10

embedded image

500.3
2.99
SB

AL11

embedded image

524.4
3.40
SB

embedded image

TABLE 133

obs

Measure-

Ex.

MS
tR
ment

No.
—B
[M + 1]
(min)
Method

AM1

embedded image

560.6
3.84
SA

AM2

embedded image

560.5
3.74
SA

AM3

embedded image

544.5
3.56
SA

AM4

embedded image

544.5
3.58
SA

AM5

embedded image

544.5
3.56
SA

AM6

embedded image

556.5
3.5
SA

AM7

embedded image

556.5
3.56
SA

AM8

embedded image

556.5
3.54
SA

TABLE 134

obs

Measure-

Ex.

MS
tR
ment

No.
—B
[M + 1]
(min)
Method

AM9

embedded image

594.4
3.77
SA

AM10

embedded image

594.4
3.87
SA

AM11

embedded image

594.4
3.88
SA

AM12

embedded image

551.5
3.46
SA

AM13

embedded image

551.6
3.48
SA

AM14

embedded image

608.5
3.92
SA

AM15

embedded image

608.5
3.97
SA

AM16

embedded image

608.5
3.95
SA

TABLE 135

obs

Measure-

Ex.

MS
tR
ment

No.
—B
[M + 1]
(min)
Method

AM17

embedded image

558.6
3.68
SA

AM18

embedded image

558.5
3.66
SA

AM19

embedded image

558.6
3.64
SA

AM20

embedded image

574.6
3.8
SA

AM21

embedded image

574.6
3.82
SA

AM22

embedded image

574.6
3.79
SA

AM23

embedded image

565.5
3.55
SA

AM24

embedded image

565.5
3.52
SA

TABLE 136

obs

Measure-

Ex.

MS
tR
ment

No.
—B
[M + 1]
(min)
Method

AM25

embedded image

570.6
3.67
SA

AM26

embedded image

570.6
3.6
SA

AM27

embedded image

570.6
3.58
SA

AM28

embedded image

464.5
3.1
SA

AM29

embedded image

478.5
3.27
SA

AM30

embedded image

492.5
3.42
SA

AM31

embedded image

596.5
3.73
SA

AM32

embedded image

541.5
2.91
SA

TABLE 137

obs

Measure-

Ex.

MS
tR
ment

No.
—B
[M + 1]
(min)
Method

AM33

embedded image

557.5
3.38
SA

AM34

embedded image

595.4
3.62
SA

AM35

embedded image

561.6
3.49
SA

AM36

embedded image

595.5
3.68
SA

AM37

embedded image

566.4
3.83
SA

AM38

embedded image

532.6
3.51
SA

AM39

embedded image

554.6
3.78
SA

TABLE 138

obs

Measure-

Ex.

MS
tR
ment

No.
—B
[M + 1]
(min)
Method

AN1

embedded image

596.5
4.01
SA

AN2

embedded image

596.5
4.13
SA

AN3

embedded image

596.4
4.14
SA

AN4

embedded image

580.4
3.91
SA

AN5

embedded image

580.4
3.97
SA

AN6

embedded image

592.4
3.81
SA

AN7

embedded image

592.4
3.95
SA

AN8

embedded image

592.6
3.92
SA

TABLE 139

obs

Measure-

Ex.

MS
tR
ment

No.
—B
[M + 1]
(min)
Method

AN9

embedded image

630.5
4.1
SA

AN10

embedded image

630.5
4.21
SA

AN11

embedded image

630.3
4.25
SA

AN12

embedded image

587.6
3.76
SA

AN13

embedded image

587.6
3.82
SA

AN14

embedded image

587.4
3.84
SA

AN15

embedded image

598.6
3.99
SA

AN16

embedded image

598.6
4.06
SA

TABLE 140

obs

Measure-

Ex.

MS
tR
ment

No.
—B
[M + 1]
(min)
Method

AN17

embedded image

598.3
3.99
SA

AN18

embedded image

598.5
4.08
SA

AN19

embedded image

598.6
3.9
SA

AN20

embedded image

594.4
3.9
SA

AN21

embedded image

610.3
4.06
SA

AN22

embedded image

500.6
3.32
SA

AN23

embedded image

514.6
3.45
SA

AN24

embedded image

528.5
3.6
SA

TABLE 141

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

AN25

embedded image

526.6
3.53
SA

AN26

embedded image

568.6
3.76
SA

AN27

embedded image

563.5
3.47
SA

AN28

embedded image

528.5
3.64
SA

TABLE 142

Ex.

obs MS
tR
Measurement

No.
—B
[M + 1]
(min)
Method

AO1

embedded image

559.4
3.23
SA

AO2

embedded image

559.6
3.78
SA

AO3

embedded image

559.6
3.69
SA

AO4

embedded image

575.5
3.81
SA

AO5

embedded image

575.5
3.88
SA

AO6

embedded image

575.6
3.86
SA

AO7

embedded image

571.5
3.79
SA

AO8

embedded image

571.5
3.66
SA

TABLE 143

Ex.

obs MS
tR
Measurement

No.
—B
[M + 1]
(min)
Method

AO9

embedded image

571.5
3.58
SA

AO10

embedded image

566.5
3.52
SA

AO11

embedded image

566.7
3.66
SA

AO12

embedded image

566.5
3.61
SA

AO13

embedded image

577.6
3.65
SA

AO14

embedded image

577.6
3.81
SA

AO15

embedded image

577.9
3.69
SB

AO16

embedded image

577.5
3.8
SA

TABLE 144

Mea-

sure-

ment

Ex.

obs MS
tR
Me-

No.
—B
[M + 1]
(min)
thod

AO17

embedded image

577.9
4.01
SB

AO18

embedded image

569.8
3.9
SB

AO19

embedded image

569.8
3.95
SB

AO20

embedded image

573.7
3.8
SB

AO21

embedded image

573.7
3.82
SB

AO22

embedded image

589.4
4.01
SB

AO23

embedded image

585.7
3.86
SB

AO24

embedded image

585.5
3.17
SB

TABLE 145

Ex.

obs MS
tR
Measurement

No.
—B
[M + 1]
(min)
Method

AO25

embedded image

573.4
3.24
SB

AO26

embedded image

589.4
3.32
SB

AO27

embedded image

589.4
3.36
SB

AO28

embedded image

585.7
3.76
SB

AO29

embedded image

556.3
2.42
SB

AO30

embedded image

610.4
3.28
SB

AO31

embedded image

572.3
2.82
SB

AO32

embedded image

560.5
2.9
SB

TABLE 146

Ex.

obs MS
tR
Measurement

No.
—B
[M + 1]
(min)
Method

AO33

embedded image

576.2
3.11
SB

AO34

embedded image

556.3
2.51
SB

AO35

embedded image

569.5
3.29
SB

AO36

embedded image

595.3
3.36
SB

AO37

embedded image

542.3
2.46
SB

TABLE 147

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

AP1

embedded image

567.3
3.63
SB

AP2

embedded image

567.3
3.42
SB

AP3

embedded image

567.3
3.65
SB

AP4

embedded image

567.3
3.97
SB

AP5

embedded image

517.4
2.92
SB

AP6

embedded image

524.4
3.32
SB

AP7

embedded image

524.4
3.36
SB

AP8

embedded image

500.3
2.99
SB

TABLE 148

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

AP9

embedded image

524.4
3.40
SB

embedded image

TABLE 149

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

AQ1

embedded image

544.5
3.58
SA

AQ2

embedded image

544.5
3.68
SA

AQ3

embedded image

544.5
3.65
SA

AQ4

embedded image

528.6
3.48
SA

AQ5

embedded image

528.6
3.51
SA

AQ6

embedded image

528.6
3.5
SA

AQ7

embedded image

540.6
3.43
SA

AQ8

embedded image

540.6
3.48
SA

TABLE 150

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

AQ9

embedded image

540.6
3.47
SA

AQ10

embedded image

578.5
3.72
SA

AQ11

embedded image

578.5
3.81
SA

AQ12

embedded image

578.6
3.83
SA

AQ13

embedded image

535.7
3.39
SA

AQ14

embedded image

535.5
3.39
SA

AQ15

embedded image

546.7
3.6
SA

AQ16

embedded image

546.7
3.57
SA

TABLE 151

Ex.

obs MS
tR
Measurement

No.
—B
[M + 1]
(min)
Method

AQ17

embedded image

592.5
3.85
SA

AQ18

embedded image

592.5
3.92
SA

AQ19

embedded image

592.4
3.9
SA

AQ20

embedded image

542.4
3.61
SA

AQ21

embedded image

542.5
3.6
SA

AQ22

embedded image

542.5
3.6
SA

AQ23

embedded image

558.6
3.7
SA

AQ24

embedded image

558.6
3.77
SA

TABLE 152

Ex.

obs MS
tR
Measurement

No.
—B
[M + 1]
(min)
Method

AQ25

embedded image

558.6
3.76
SA

AQ26

embedded image

549.5
3.49
SA

AQ27

embedded image

549.6
3.46
SA

AQ28

embedded image

554.6
3.6
SA

AQ29

embedded image

554.6
3.54
SA

AQ30

embedded image

554.6
3.53
SA

AQ31

embedded image

448.6
3.03
SA

AQ32

embedded image

462.4
3.2
SA

TABLE 153

Ex.

obs MS
tR
Measurement

No.
—B
[M + 1]
(min)
Method

AQ33

embedded image

476.4
3.49
SA

AQ34

embedded image

474.6
3.28
SA

AQ35

embedded image

580.5
3.61
SA

AQ36

embedded image

568.6
3.74
SA

AQ37

embedded image

584.5
3.9
SA

AQ38

embedded image

525.6
2.75
SA

AQ39

embedded image

541.7
3.3
SA

AQ40

embedded image

579.6
3.59
SA

TABLE 154

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

AQ41

embedded image

545.6
3.43
SA

AQ42

embedded image

579.6
3.62
SA

AQ43

embedded image

550.4
3.76
SA

AQ44

embedded image

516.7
3.43
SA

TABLE 155

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

AR1

embedded image

580.4
3.93
SA

AR2

embedded image

580.4
4.05
SA

AR3

embedded image

580.4
4.04
SA

AR4

embedded image

564.5
3.83
SA

AR5

embedded image

564.6
3.88
SA

AR6

embedded image

564.5
3.88
SA

AR7

embedded image

576.4
3.73
SA

AR8

embedded image

576.4
3.87
SA

TABLE 156

obs MS
tR
Measurement

Ex. No.
—B
[M + 1]
(min)
Method

AR9

embedded image

576.5
3.84
SA

AR10

embedded image

614.3
4.02
SA

AR11

embedded image

614.4
4.14
SA

AR12

embedded image

614.4
4.17
SA

AR13

embedded image

571.5
3.76
SA

AR14

embedded image

571.5
3.79
SA

AR15

embedded image

582.5
3.92
SA

AR16

embedded image

582.5
3.97
SA

TABLE 157

obs MS
tR
Measurement

Ex. No.
—B
[M + 1]
(min)
Method

AR17

embedded image

582.2
3.92
SA

AR18

embedded image

582.4
3.99
SA

AR19

embedded image

582.4
3.82
SA

AR20

embedded image

578.5
3.82
SA

AR21

embedded image

594.4
3.98
SA

AR22

embedded image

484.4
3.23
SA

AR23

embedded image

498.6
3.37
SA

AR24

embedded image

512.7
3.52
SA

TABLE 158

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

AR25

embedded image

510.7
3.44
SA

AR26

embedded image

552.6
3.69
SA

AR27

embedded image

547.6
3.4
SA

TABLE 159

obs MS
tR
Measurement

Ex. No.
—B
[M + 1]
(min)
Method

AS1

embedded image

543.4
3.11
SB

AS2

embedded image

543.4
3.24
SB

AS3

embedded image

543.4
3.17
SB

AS4

embedded image

559.4
3.27
SB

AS5

embedded image

559.4
3.38
SB

AS6

embedded image

559.4
3.34
SB

AS7

embedded image

555.5
3.27
SB

TABLE 160

Ex.

obs MS
tR
Measurement

No.
—B
[M + 1]
(min)
Method

AS9

embedded image

555.2
3.07
SB

AS10

embedded image

550.43
3.02
SB

AS11

embedded image

550.4
3.15
SB

AS12

embedded image

550.4
3.13
SB

AS13

embedded image

561.4
3.15
SB

AS14

embedded image

561.4
3.25
SB

AS15

embedded image

561.4
3.03
SB

TABLE 161

Measure-

Ex.

obs MS
tR
ment

No.
—B
[M + 1]
(min)
Method

AS17

embedded image

561.4
3.38
SB

AS18

embedded image

553.2
3.24
SB

AS19

embedded image

553.5
3.24
SB

AS20

embedded image

557.4
3.15
SB

AS21

embedded image

557.4
3.15
SB

AS22

embedded image

573.4
3.3
SB

AS23

embedded image

569.5
3.21
SB

TABLE 162

Mea-

sure-

Ex.

obs MS
tR
ment

No.
—B
[M + 1]
(min)
Method

AS25

embedded image

557.4
3.15
SB

AS26

embedded image

573.4
3.27
SB

AS27

embedded image

573.4
3.28
SB

AS28

embedded image

569.5
3.17
SB

AS29

embedded image

540.4
3.5
SB

AS30

embedded image

556.3
2.78
SB

AS31

embedded image

544.3
2.85
SB

AS32

embedded image

560.5
3.14
SB

TABLE 163

obs MS
tR
Measurement

Ex. No.
—B
[M + 1]
(min)
Method

AS33

embedded image

540.4
3.47
SB

AS34

embedded image

553.2
3.19
SB

AS35

embedded image

579.3
3.29
SB

AS36

embedded image

526.4
2.4
SB

TABLE 164

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

AT1

embedded image

551.3
3.55
SB

AT2

embedded image

508.4
3.19
SB

AT3

embedded image

551.3
3.86
SB

AT4

embedded image

508.4
2.92
SB

AT5

embedded image

551.3
3.30
SB

AT6

embedded image

508.4
3.21
SB

AT7

embedded image

551.3
3.21
SB

AT8

embedded image

508.4
3.32
SB

TABLE 165

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

AT9

embedded image

501.4
2.82
SB

AT10

embedded image

514.6
2.36
SB

AT11

embedded image

484.6
2.90
SB

embedded image

TABLE 166

Ex.

obs MS

Measurement

No.
—B
[M + 1]
tR (min)
Method

AU1

embedded image

544.4
3.58
SA

AU2

embedded image

544.6
3.68
SA

AU3

embedded image

544.5
3.67
SA

AU4

embedded image

528.6
3.5
SA

AU5

embedded image

528.6
3.53
SA

AU6

embedded image

528.6
3.51
SA

AU7

embedded image

540.6
3.44
SA

AU8

embedded image

540.6
3.49
SA

TABLE 167

obs MS
tR
Measurement

Ex. No.
—B
[M + 1]
(min)
Method

AU9

embedded image

540.6
3.48
SA

AU10

embedded image

578.5
3.72
SA

AU11

embedded image

578.5
3.82
SA

AU12

embedded image

578.5
3.83
SA

AU13

embedded image

535.7
3.4
SA

AU14

embedded image

535.6
3.39
SA

AU15

embedded image

546.7
3.58
SA

AU16

embedded image

546.7
3.76
SA

TABLE 168

obs MS
tR
Measurement

Ex. No.
—B
[M + 1]
(min)
Method

AU17

embedded image

546.7
3.59
SA

AU18

embedded image

592.5
3.85
SA

AU19

embedded image

592.5
3.91
SA

AU20

embedded image

592.5
3.87
SA

AU21

embedded image

542.5
3.61
SA

AU22

embedded image

542.5
3.62
SA

AU23

embedded image

542.5
3.6
SA

AU24

embedded image

558.6
3.88
SA

TABLE 169

Ex.

obs MS
tR
Measurement

No.
—B
[M + 1]
(min)
Method

AU25

embedded image

558.6
3.77
SA

AU26

embedded image

558.5
3.76
SA

AU27

embedded image

549.7
3.47
SA

AU28

embedded image

549.6
3.45
SA

AU29

embedded image

554.6
3.61
SA

AU30

embedded image

554.6
3.55
SA

AU31

embedded image

554.6
3.53
SA

AU32

embedded image

448.6
3.05
SA

TABLE 170

Ex.

obs MS
tR
Measurement

No.
—B
[M + 1]
(min)
Method

AU33

embedded image

462.5
3.21
SA

AU34

embedded image

476.4
3.37
SA

AU35

embedded image

474.4
3.28
SA

AU36

embedded image

580.5
3.61
SA

AU37

embedded image

584.5
3.9
SA

AU38

embedded image

525.7
2.76
SA

AU39

embedded image

541.5
3.3
SA

AU40

embedded image

579.6
3.55
SA

TABLE 171

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

AU41

embedded image

545.6
3.43
SA

AU42

embedded image

579.6
3.63
SA

AU43

embedded image

550.5
3.75
SA

AU44

embedded image

516.7
3.42
SA

TABLE 172

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

AV1

embedded image

580.4
3.94
SA

AV2

embedded image

580.4
4.07
SA

AV3

embedded image

580.4
4.06
SA

AV4

embedded image

564.4
3.83
SA

AV5

embedded image

564.4
3.9
SA

AV6

embedded image

564.6
3.88
SA

AV7

embedded image

576.5
3.74
SA

AV8

embedded image

576.5
3.88
SA

TABLE 173

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

AV9

embedded image

576.5
3.84
SA

AV10

embedded image

614.4
4.03
SA

AV11

embedded image

614.3
4.14
SA

AV12

embedded image

614.4
4.17
SA

AV13

embedded image

571.5
3.71
SA

AV14

embedded image

571.5
3.76
SA

AV15

embedded image

571.5
3.79
SA

AV16

embedded image

582.3
3.92
SA

TABLE 174

Ex.

obs MS

Measurement

No.
—B
[M + 1]
tR (min)
Method

AV17

embedded image

582.3
3.98
SA

AV18

embedded image

582.3
3.93
SA

AV19

embedded image

582.4
4.01
SA

AV20

embedded image

582.3
3.97
SA

AV21

embedded image

578.5
3.83
SA

AV22

embedded image

594.4
4
SA

AV23

embedded image

484.5
3.4
SA

AV24

embedded image

498.6
3.37
SA

TABLE 175

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

AV25

embedded image

512.7
3.51
SA

AV26

embedded image

510.5
3.46
SA

AV27

embedded image

552.4
3.7
SA

AV28

embedded image

547.6
3.4
SA

TABLE 176

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

AW1

embedded image

543.4
3.11
SB

AW2

embedded image

543.4
3.24
SB

AW3

embedded image

543.4
3.15
SB

AW4

embedded image

559.6
3.74
SA

AW5

embedded image

559.5
3.81
SA

AW6

embedded image

559.5
3.79
SA

AW7

embedded image

555.2
3.21
SB

TABLE 177

Measure-

Ex.

obs MS
tR
ment

No.
—B
[M + 1]
(min)
Method

AW9

embedded image

555.5
3.07
SB

AW10

embedded image

550.7
3.08
SA

AW11

embedded image

550.7
3.18
SA

AW12

embedded image

550.7
3.69
SA

AW13

embedded image

561.7
3.58
SA

AW14

embedded image

561.4
3.32
SA

AW15

embedded image

561.4
3.13
SB

TABLE 178

Mea-

sure-

ment

Ex.

obs MS
tR
Meth-

No.
—B
[M + 1]
(min)
od

AW17

embedded image

561.7
3.96
SB

AW18

embedded image

553.8
3.9
SB

AW19

embedded image

553.5
3.86
SB

AW20

embedded image

557.7
3.72
SB

AW21

embedded image

557.7
3.76
SB

AW22

embedded image

573.4
3.28
SB

AW23

embedded image

569.5
3.15
SB

TABLE 179

Mea-

obs

sure-

MS

ment

Ex.

[M +
tR
Meth-

No.
—B
1]
(min)
od

AW25

embedded image

569.5
3.1
SB

AW26

embedded image

557.4
3.23
SB

AW27

embedded image

573.4
3.26
SB

AW28

embedded image

573.4
3.29
SB

AW29

embedded image

540.4
2.4
SB

AW30

embedded image

594.4
3.21
SB

AW31

embedded image

556.3
2.73
SB

AW32

embedded image

544.6
2.86
SB

TABLE 180

obs MS
tR
Measurement

Ex. No.
—B
[M + 1]
(min)
Method

AW33

embedded image

560.5
3.05
SB

AW34

embedded image

540.4
2.44
SB

AW35

embedded image

579.3
3.34
SB

AW36

embedded image

526.4
2.38
SB

AW37

embedded image

553.2
3.21
SB

TABLE 181

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

AX1

embedded image

551.3
3.55
SB

AX2

embedded image

551.3
3.30
SB

AX3

embedded image

551.3
3.54
SB

AX4

embedded image

551.3
3.84
SB

AX5

embedded image

501.4
2.82
SB

AX6

embedded image

508.4
3.19
SB

AX7

embedded image

508.4
3.20
SB

AX8

embedded image

484.6
2.90
SB

TABLE 182

obs MS

Measurement

Ex. No.
—B
[M + 1]
tR (min)
Method

AX9

embedded image

508.4
3.30
SB

Comparative Example 1A

embedded image

To a solution of Compound I (6.91 g) in 99.5% ethanol (100 mL) was added sodium bicarbonate (3.31 g), and then thereto was added ethyl bromopyruvate (3.0 mL) and then the mixture was stirred at 80° C. After 4 hours, thereto was added acetic acid (50 mL), and the mixture was stirred at 120° C. The mixture was stirred overnight, and then cooled to room temperature and concentrated in vacuo. To the residue was added saturated sodium bicarbonate water, and the mixture was extracted with ethyl acetate. The organic layer was dried over sodium sulfate and concentrated in vacuo. The residue was purified by silica gel chromatography (eluent: hexane/ethyl acetate=1/1 to chloroform/methanol=10/1) to give the titled Compound II (2.48 g) (30.4% yields).

Comparative Example 1B

embedded image

To a solution of Compound I (6.91 g) in 99.5% ethanol (100 mL) was added sodium bicarbonate (3.31 g). Then, thereto was added ethyl bromopyruvate (3.0 mL), and then the mixture was stirred at 80° C. After 4 hours, thereto was added acetic acid (50 mL), and the mixture was stirred at 120° C. The mixture was stirred overnight, and then cooled to room temperature and concentrated in vacuo. To the residue was added saturated sodium bicarbonate water, and the mixture was extracted with ethyl acetate. The organic layer was dried over sodium sulfate and concentrated in vacuo. The residue was purified by silica gel chromatography (eluent: hexane/ethyl acetate=1/1 to chloroform/methanol=10/1) to give the titled Compound II (2.48 g) (30.4% yields).

Comparative Example 2

embedded image

To an ice-cooled mixed solution of Compound I (24.9 g), sodium bicarbonate (9.66 g) and 95% ethanol (350 mL) was added ethyl bromopyruvate (11.9 mL). The mixture was stirred at 80° C. for 4 hours, and the reaction solution was evaporated to concentrate in vacuo. To the residue was added acetic acid (150 mL), and the mixture was stirred at 130° C. for 18 hours and then concentrated in vacuo. To the residue was added saturated sodium bicarbonate water, and the mixture was extracted with ethyl acetate. The organic layer was dried over sodium sulfate and concentrated in vacuo. The residue was purified by silica gel chromatography (eluent: hexane/ethyl acetate=1/1) to give the titled Compound II (14.6 g) (49% yields).

Comparative Example 3

embedded image

To an ice-cooled solution of Compound I (16.44 g) in 99.5% ethanol (125 mL) was added ethyl bromopyruvate (7.83 mL), and then the mixture was stirred at 80° C. After 4 hours, the reaction solution was evaporated to concentrate in vacuo. To the residue was added acetic acid (150 mL), and the mixture was stirred at 130° C. for 18 hours and then concentrated in vacuo. To the residue was added saturated sodium bicarbonate water, and the mixture was extracted with ethyl acetate. The organic layer was dried over sodium sulfate and concentrated in vacuo. The residue was purified by silica gel chromatography (eluent: hexane/ethyl acetate=1/1) to give the titled Compound II (1.43 g) (7% yields).

Experiment 1 (Preparation of Human 11βHSD1 Enzyme Source)

A sequence containing ORF of human 11βHSD1 gene (GenBank Accession No. BC012593) was amplified according to PCR technique which was one of conventional methods, and digested by a restriction enzyme BamHI/XhoI. 2 kb of DNA fragments obtained from agarose gels were inserted into pCMV-Tag 2B plasmids (Stratagene) according to a conventional method. The plasmids prepared in Escherichia coli in large amounts were transformed into CHO-K1 cells, and then cells stably expressing human 11βHSD1 genes were selected by medium containing 400 μg/ml G-418 solution (GIBCO, Inc.) containing media. The resulting stably expressing cells were incubated up to −90% confluent in F-12 medium (Nacalai Tesque, Inc.) containing 10% charcoal-dextran treated fetal bovine serum (Hyclone), 1% penicillin-streptomycin (Nacalai Tesque, Inc.) and 400 μg/ml G-418. The resultant cells were treated by tripsin, and the obtained cells were suspended in the above media (1 L) and seeded in cell stack 10 chamber (Corning) in total amounts. The mixture was incubated in CO₂incubator (5% CO₂, 37° C.) for 4-5 days, and then treated by tripsin. Total amounts of the obtained cells were washed with PBS buffer (GIBCO, Inc.), and stored at −80° C. The cells were suspended in 8 ml of buffer (50 mM HEPES pH7.3, 5% glycerol, 1 mM EDTA, protease inhibitor cocktail (Roche)), and then disrupted. The resulting solution was centrifuged at 1,500 rpm for 10 minutes, and then the supernatant was ultracentrifuged at 100,000 g for 1 hour. The precipitate after the ultracentrifugation was collected and suspended in buffer (50 mM HEPES pH7.3, 5% glycerol, 1 mM EDTA), and then dispensed to store at −80° C. The resulting enzyme fractions were used as human 11βHSD1 enzyme fractions in the following Experiments.

Experiment 2 (Measurement of Human 11βHSD1 Inhibitory Activity)

A test compound and cortisone (Sigma) was diluted with buffer (50 mM HEPES pH7.3, 150 mM NaCl, 1 mM EDTA) to prepare a substrate solution containing a test compound (50 mM HEPES pH7.3, 150 mM NaCl, 1 mM EDTA, 1 mM NADPH, 20 nM cortisone) (2% DMSO solution), and the solution was added to 384-well low-volume plate (manufactured by Greiner, No. 3782086) in 4 μl/well. Then, human 11βHSD1 enzyme fractions obtained in Experiment 1 were diluted with buffer (50 mM HEPES pH7.3, 150 mM NaCl, 1 mM EDTA, 5% glycerol) to be 60-100 μg/ml of post-assay concentrations. Human 11βHSD1 enzyme fractions after the dilution was added to each well in 4 μl/well and gently stirred, and then spun down to react at 37° C. for 2 hours. After the enzyme reaction, the produced cortisol was detected by Homogeneous time-resolved fluorescence (HTRF) to determine enzyme inhibitory activities. Then, thereto were added XL-665 labeled cortisol (or d2-labeled cortisol) containing 400 μM carbenoxolone (Sigma) and cryptate-labeled cortisol antibody (Cisbio International) in 4 μl/well each, and the mixture was gently stirred, and then spun down to store at room temperature for 2 or more hours. A fluorescence intensity was determined by 2120 EnVision® Multilabel counter (PerkinElmer) to calculate enzyme inhibitory activities from 2 wavelengths of fluorescence intensity ratios (665 nm/620 nm).

Each inhibitory activity (%) of each test compound was calculated from the average value (%) of inhibitory activities of 4 wells under the same condition. An inhibition rate in a well wherein DMSO was added instead of a test compound was 0% and an inhibition rate in a well without human 11βHSD1 enzyme fractions was 100%. A concentration (IC₅₀value) of a test compound required to inhibit 50% of human 11βHSD1 was calculated.

The result was shown in Table 1.

TABLE 183

Example No.
IC₅₀(nM)

1
11
32

2
15
39

3
19
25

Experiment 3 (Inhibitory Activity Assay for Cortisone Reducing Activity of Cultured Human Adipocytes)

Normal human proadipocytes (HPrAD-vis, Cambrex) were inoculated on 48 well plate, and differentiated according to a protocol attached to a kit. Media for cells on 9-11th day of differentiation were changed to 0.2 ml of D-MEM media (GIBCO, Inc.) containing 100 nM [1,2-³H] cortisone (1 μCi/well, Muromati Yakuhin), 0.5% DMSO, test compound (DMSO only for test compound-addition districts and test compound additive-free districts). After incubation at 37° C. for 3 hours, all media were collected. As background districts, cell additive-free media were used. Media were mixed with ethyl acetate (0.1 ml) in Eppendorf tube. The mixture was vortexed, and then centrifuged at 5,000 rpm×1 minute at room temperature to separate ethyl acetate (upper layer). Ethyl acetate (10 μl) was spotted on aluminum plate for thin-layer chromatography (silica gel 60 angstrom, Merck, referred to as TLC plate hereinafter). To a sealed vessel was added chloroform/methanol (90:10, v/v) as an eluent, and TLC plate was developed and then dried at room temperature. To the dried TLC plate was exposed an imaging plate (TR-2040, FUJIFILM) over 16 or more hours. After exposure, the imaging plate was analyzed by Bioimage analyzer (BAS2500, FUJIFILM), and [³H] radioactivity of the spot corresponding to cortisol on TLC plate was determined Inhibitory activities of cortisone reducing activities of test compounds were calculated as below.

(Inhibitory activity (%))=100×((Test compound additive-free districts)−(Test compound-addition districts))/((Test compound additive-free districts)−(Background districts))

IC₅₀values were calculated by a linear regression of logarithmic values of analyte concentrations and inhibitory activity values using 2-point data wherein inhibitory activities indicated values around 50%. IC₅₀values for human adipocyte cortisone reducing activities of the inventive compound usually exist within the range of 0.01-1000 nM. IC₅₀values for human adipocyte cortisone reducing activities of the following inventive compounds were determined

TABLE 184

Example No.
IC₅₀(nM)

1
8
8.7

2
22
1.8

3
65
9.1

4
66
0.3

5
70
39

6
82
43

7
129
3.5

8
153
22

9
160
5.6

10
166
6.4

11
K16
11

12
I26
3.1

13
V18
21

14
AN2
6.7

According to the experiment of Table 2, the inventive compound group is expected to inhibit an 11βHSD1 activity and cortisol production in the target organ human adipocyte.

Experiment 4 (Inhibitory Activity Assay for Cortisone Reducing Activity of Mouse Primary Adipocytes)

Adipose tissues (referred to as visceral fat tissues hereinafter) adhered around mesenteries and testicles of 10 of 9-11 week-old ICR male mice (Japan SLC, Inc.) were soaked in phosphate buffer (0.20 g/L KCl, 0.20 g/L KH₂PO₄, 8.00 g/L NaCl, 2.16 g/L Na₂HPO₄.7H₂O, 100 unit/ml penicillin (GIBCO, Inc.), 100 μg/ml streptomycin (GIBCO, Inc.), 250 ng/ml amphotericin (GIBCO, Inc.)) (about 100 ml) and washed at room temperature.

The visceral fat tissues removed as above were cut out in about 5×5 mm by scissors in Dulbecco's Modified Eagle Media (containing 4.5 g/L D-glucose and 584 mg/L L-glutamine, GIBCO, Inc.) (about 50 ml) wherein collagenase (type II, Sigma), penicillin (GIBCO, Inc.), streptomycin (GIBCO, Inc.) and amphotericin (GIBCO, Inc.) were added until the final concentration of 1 mg/ml, 100 unit/ml, 100 μg/ml and 250 ng/ml each. Then, the tissues were shaken at 37° C. for 30 minutes (about 170 rpm) and filtered through nylon mesh (80S [250 μm mesh], SANSHIN INDUSTRIAL CO., LTD.) to give a filtrate (cell suspension). The filtrate was centrifuged at room temperature at 1800 rpm for 5 minutes, and then the liquid layer was gently removed by decantation to give a precipitate. The precipitate was suspended in Dulbecco's Modified Eagle Media (containing 4.5 g/L D-glucose and 584 mg/L L-glutamine, GIBCO, Inc., also referred to as FBS-containing media hereinafter) (30 ml) wherein fetal bovine serum (referred to as FBS hereinafter) (GIBCO, Inc.), ascorbic acid (Wako Pure Chemical Industries, Ltd.), penicillin (GIBCO, Inc.), streptomycin (GIBCO, Inc.) and amphotericin (GIBCO, Inc.) were added until the final concentration of 10%, 200 μM, 100 unit/ml, 100 μg/ml and 250 ng/ml each, and the suspension was filtered through nylon mesh (420S [25 μm mesh], SANSHIN INDUSTRIAL CO., LTD.). The filtrate was collected and centrifuged at room temperature at 1800 rpm for 5 minutes, and then the liquid layer was gently removed by decantation and the precipitate was suspended again in FBS-containing media (30 ml). The similar treatment of centrifugation, removal of liquid layer and suspension in FBS-containing media was further carried out twice for the resulting suspension to prepare the suspension (90 ml). The suspension was dispensed in flasks for cell incubation (T150 for adhered cells, IWAKI GLASS) by 30 ml each, and incubated at 37° C. in the presence of 5% CO₂. 5-6 hours after starting incubation, media were removed and flask walls were washed with the phosphate buffer (15 ml). The washing was removed and the washing operation was carried out again. Then, the phosphate buffer was removed, and FBS-containing media (30 ml) was added to flasks and incubated at 37° C. in the presence of 5% CO₂. 1 or 2 days after starting incubation, media were removed and flask walls were washed with the phosphate buffer (15 ml) once. Then, to the flask was added tripsin-ethylene diamine tetracetic acid (referred to as tripsin-EDTA hereinafter) solution (0.05% tripsin, 0.53 mM EDTA.4Na, GIBCO, Inc.) so that cells were soaked, and the mixture was incubated at 37° C. for 5 minutes. Then, thereto were added FBS-containing media in about tenfold amounts of tripsin-EDTA solution, and the cell suspension was obtained.

The cell suspension was diluted by the addition of FBS-containing media so that the number of cells in the cell suspension was determined by a counting chamber to be 1.4×10⁵cells/ml. The resulting diluent was dispensed in 48 well plate (for incubation of adherent cells, IWAKI GLASS) by 300 μl/well each, and incubated at 37° C. for 1-2 days in the presence of 5% CO₂. Media were removed from each well of 48 well plate, and FBS-containing media (300 μl) containing 10 μg/ml insulin (Sigma), 0.25 μM dexamethazone (Wako Pure Chemical Industries, Ltd.), 0.5 mM 3-isobutyl-1-methyl-xanthin (Sigma) and 5 μM 15-deoxy-Δ^12,14-prostaglandin J₂(Cayman) were added to each well and incubated at 37° C. for 3 days in the presence of 5% CO₂. Then, media in each well were removed, and FBS-containing media (300 μl) containing 10 μg/ml insulin and 5 μM 15-deoxy-Δ^12,14-prostaglandin J₂were added to each well and incubated for 2 days. Further, media in each well were removed, and FBS-containing media (300 μl) containing 10 μg/ml insulin and 5 μM 15-deoxy-Δ^12,14-prostaglandin J₂were added to each well and incubated for 2 days.

Media for adipocyte as differentiated above were changed to 0.2 ml of D-MEM media (GIBCO, Inc.) containing 100 nM [1,2-³H] cortisone (1 μCi/well, Muromati Yakuhin), 0.5% DMSO, test compound (DMSO only for test compound-addition districts and test compound additive-free districts). After incubation at 37° C. for 3 hours, all media were removed. As background districts, cell additive-free media were used. Media were combined with ethyl acetate (0.1 ml) in Eppendorf tube. The mixture was vortexed, and then centrifuged at 5,000 rpm×1 minute at room temperature to separate ethyl acetate (upper layer). Ethyl acetate (10 μl) was spotted on aluminum plate for thin-layer chromatography (silica gel 60 angstrom, Merck, referred to as TLC plate hereinafter). To a sealed vessel was added chloroform/methanol (90:10, v/v) as an eluent, and TLC plate was developed and then dried at room temperature. To the dried TLC plate was exposured an imaging plate (TR-2040, FUJIFILM) over 16 or more hours. After exposure, the imaging plate was analyzed by Bioimage analyzer (BAS2500, FUJIFILM), and [³H] radioactivity of the spot corresponding to cortisol on TLC plate was determined Inhibitory activities of cortisone reducing activities of test compounds were calculated as below.

(Inhibitory activity (%))=100×((Test compound additive-free districts)−(Test compound-addition districts))/((Test compound additive-free districts)−(Background districts))

IC₅₀values were calculated by a linear regression of logarithmic values of analyte concentrations and inhibitory activity values using 2-point data wherein inhibitory activities indicated values around 50%. IC₅₀values for mouse adipocyte cortisone reducing activities of the inventive compound usually exist within the range of 0.01-1000 nM. IC₅₀values for mouse adipocyte cortisone reducing activities of the following inventive compounds were determined. The results are shown below.

TABLE 185

Example No.
IC₅₀(nM)

1
51
5.6

2
62
47

3
64
2.4

4
66
0.6

5
74
4.0

6
93
1.5

7
96
55

8
169
4

9
A41
<10

10
Y9
4.2

The inventive compound has good properties as a medicinal product. The properties include solubility which may be measured according to methods of Experiments 5-1 and 5-2 or other known methods.

Experiment 5-1 (Elution Method)

1.75% aqueous disodium hydrogen phosphate solution was mixed with 5.53% aqueous citric acid solution with monitoring by pH indicator to prepare isotonic buffer solutions of pH=7.4 and 6.0. A buffer of pH=1.2 (Pharmacopeia Solution 1) was prepared according to Pharmacopeia. Then, a standard solution was prepared. A test compound (about 1 mg) was precisely weighed in 10 ml measuring flask and dissolved in HPLC carrier (0.1% TFA water/acetonitrile=1/1) to prepare 100 μg/ml standard solution. An elution condition for a test compound was set by the standard solution in ODS column (ChemcoPack Quicksorb: 4.6 mmφ×150 mm, 5 μm) at 5-10 min. Detection was carried out by UV at both 254 and 230 nm of wavelengths. Quantification was carried out on the basis of the former detected data, and in case of a low sensitibity, the latter detected data was adopted. Dissolution and analysis were carried out as follows. A test compound (about 1 mg) was weighed in 1 ml glass sample tube, and thereto was added each pH of isotonic buffer solution (0.4 ml) by PIPETMAN® and the mixture was shaken at room temperature for 1.5 hours (Conditions: RECIPRO SHAKER SR-1N manufactured by TAITEC, Speed=8). Then, the solution was transferred to 1.5 ml Eppendorf tube, and centrifuged by a compact high-speed centrifuge at 15.000 rpm for 5 minutes to separate an insoluble. The supernatant was analyzed by HPLC without any purification to calculate a concentration (solubility) by area ratios with a standard solution.

Experiment 5-2 (Dimethylsulfoxide (Abbreviated as DMSO Hereinafter) Deposition Method)

1.75% aqueous disodium hydrogen phosphate solution was mixed with 5.53% aqueous citric acid solution with monitoring by pH indicator to prepare each isotonic buffer solution of pH=7.4. A buffer of pH=1.2 (Pharmacopeia Solution 1) was prepared according to Pharmacopeia. Then, a standard solution was prepared. A test compound (2 μL, 10 mM DMSO solution) was dispensed in 96 well plate and diluted with 50% acetonitrile (198 μL). A HPLC analysis condition was determined by the standard solution. The analysis was carried out under HPLC (Column: ACQUITY HPLC BEH® C18 1.7 μm 2.1 mm×50 mm, Guard column: VanGuard® Pre-column 2.1×5 mm, Mobile phase: solution A; 0.1% TFA aqueous solution, solution B; 0.1% TFA acetonitrile solution, Gradient: 0.00 min-solution B: 5%, 2.00 min-solution B: 100%, 2.71 min-solution B: 5%, 3.50 min-stop, Column temperature: 40° C., flow rate: 0.4 mL/min, Detection wavelength: 254 or 230 nm, Sample injection: 5 μL), and a measurement wavelength and injection amounts of analysis were determined by the result. Dissolution and analysis were carried out as follows. Samples (10 mM DMSO solution) were dispensed in four Utube on 96 well rack by 15 μL, and evaporate to dryness by centrifugal evaporation at 40° C. for 90 minutes. Thereto was added DMSO (3 μL) to dissolve again, and then buffers of pH7.4 and 1.2 were added to 2 wells each in 300 μL each. After shaking at 25° C. at 110 rpm for 90 minutes, the mixture let stand for 16-20 hours and centrifuged at 2000 g for 15 minutes to separate an insoluble and collect a supernatant (100 μL) in 96 well plate. A test compound (2 μL, 10 mM DMSO solution) was dispensed in separate 96 well plate and diluted with 50% acetonitrile (198 μL) to prepare 100 μM standard solution. Additionally, 100 μM standard solution was tenfold diluted with 50% acetonitrile to prepare 10 μM standard solution. The sample for measuring solubility and two standard solutions were analyzed under the measurement condition determined in pre-investigation to calculate solubilities by area ratios with a standard solution.

INDUSTRIAL APPLICABILITY

The inventive compound is useful as a preventive and/or therapeutic agent for a disease including type II diabetes, abnormal glucose tolerance, hyperglycemia, insulin resistance, hypo-HDL-emia, hyper-LDL-emia, dyslipidemia, hyperlipidemia, hypertriglyceridemia, hypercholesterolemia, hypertension, arteriosclerosis, angiostenosis, atherosclerosis, obesity, cognitive disorder, glaucoma, retinopathy, dementia, Alzheimer disease, osteoporosis, immune disorder, syndrome X, depression, cardiovascular disease, neurodegenerative disease, etc.

Number	Date	Country	Kind
2007-204739	Aug 2007	JP	national
2008-074895	Mar 2008	JP	national

AMINOPYRAZOLE AMIDE DERIVATIVE

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