Patent Application
20090082558
This invention discloses a stable and novel amorphous form of rifaximin prepared by the judicious combination of dissolution solvent and anti-solvent.
Rifaximin (1) {(2S,16Z,18E,20S,21S,22R,23R,24R,25S,26S,27S,28E)-5,6,21,23,25-pentahydroxy-27-methoxy-2,4,11,16,20,22,24,26-octamethyl-2,7-(epoxypentadeca-[1,11,13]trienimino)benzofuro[4,5-e]pyrido[1,2-α]-benzimidazole-1,15(2H)-dione,25-acetate} is a semisynthetic rifamycin-based non-systemic antibiotic marketed in the US as Xifaxan® by Salix Pharmaceuticals.
It is useful for the treatment of travelers diarrhea in adults and in children 12-years of age and older caused by E. Coli bacteria. Rifaximin has also been evaluated for the treatment of irritable bowel syndrome, diverticular disease, hepatic encephalopathy, pyogenic skin infections, and as an antibacterial prophylactic prior to colon surgery. Medical News Today has recently published an article detailing a study conducted by physicians at New York-Presbyterian Hospital/Weill Cornell Medical Center using rifaximin for the treatment of patients who suffer from severe Crohn's disease and who have not responded to all other available medications.
Structurally rifaximin is a pyrido-imidazo derivative of rifamycin SV (4-deoxy-4′-methylpyrido[1′,2′:1,2]imidazo[5,4-c]rifamycin SV). Unlike other rifamycin SV derivatives, rifaximin exerts broad spectrum activity and has a specific mode of action which results in low gastrointestinal absorption.
GB 2,079,270 disclose imidazo-rifamycin derivatives having antibacterial activity, prepared from 3-halorifamycin S. U.S. Pat. No. 4,341,785 and EP 0,161,534 (Alpha Farmaceutici SpA) describe the processes for preparing pyrido-imidazo rifamycin starting from rifamycin O. The above patents detail a generic method for the purification of rifaximin in suitable solvent systems such as methylene chloride, chloroform, methanol, ethanol, isopropanol. Water is generally used as an anti-solvent. The polymorphic form of the rifaximin obtained by these methods was not disclosed.
Recently, three polymorphic forms of rifaximin were described in U.S. Pat. No. 7,045,620 (Alfa Wassermann SpA) and designated as α, β, γ. The point of differentiation between these forms is their respective water contents and powder X-ray diffraction (PXRD) diffractogram. These forms are inter-convertible and, therefore, obtaining a specific polymorphic form is dependent on the drying conditions. The γ-form of U.S. Pat. No. 7,045,620 is described as being poorly crystalline with a high content of amorphous component. It is characterized as having a water content between 1.0% and 2.0% and having a PXRD diffractogram containing a few significant peaks at 5.0, 7.1 and 8.4 (two-theta). In particular, this form is prepared by dissolution of in ethanol followed by the addition of water. Also noteworthy is that this form is prone to conversion to other polymorphic forms on exposure to, for instance, due to the change in its water content level. This is a disadvantage since it is highly desirable to have an active pharmaceutical ingredient which is polymorphically stable and suitable for pharmaceutical applications (see Konne, T., Chem. Pharma. Bull., 38, p. 2003, (1990)).
EP 1,698,630 (Alfa Wassermann SpA) teaches two new polymorphic forms (δ, ε). These forms are also obtained from the same process method described above and their specific crystal structures are again dependent of water content and there is a degree of overlap with the other purported forms.
Therefore, it is an object of the present invention to provide a polymorphically pure and stable form of rifaximin, prepared by utilizing industrially acceptable procedures, which overcomes the deficiencies of the prior art.
Further and other objects of the invention will become apparent to those skilled in the art when considering the following summary of the invention and the more detailed description of the embodiments of the invention described herein.
According to one aspect of the present invention there is disclosed a stable amorphous form of rifaximin. Preferably, the stable amorphous form of rifaximin is characterized by having X-ray powder diffraction pattern peaks, as reported in
According to another aspect of the invention there is provided a process for the preparation of stable amorphous form of rifaximin which comprises:
The present invention uses industrially acceptable class-3 solvents, as listed in the ICH Q3C(R3) guidelines, throughout the process. In contrast to the previous methods, the method of the instant invention does not use water which is a disadvantage for a hygroscopic material.
In the preferred embodiments of this invention, the crude rifaximin is dissolved in solvents selected from C3 to C6 alkyl esters, for instance methyl acetate, ethyl acetate, isopropyl acetate; C1-C6 lower alkanols, for instance ethanol, isopropanol, 2-butanol; and C3-C6 alkyl ketones, for instance, acetone, methyl iso-butyl ketone (MIBK).
The temperature of this solution could be between 0° C. to reflux, preferably 10 to 55° C. and most preferably 15-45° C. The volumes used can be 1 to 25 volumes, preferably 1.5 to 12 volumes and most preferably 2 to 6 volumes. Optionally, the solution could be clarified by, for instance, filtration through a Buchner funnel or cartridge filter.
The anti-solvent is added to rifaximin containing solution in order to precipitate the amorphous rifaximin. Suitable anti-solvents include C5 to C10 hydrocarbons, such as heptanes; C4 to C8 alkyl ethers, such as methyl tert-butyl ether; or mixtures thereof. The most preferable anti-solvents are heptanes and methyl tert-butyl ether (MTBE), or mixtures thereof. The volume of antisolvent is between 1 to 36 volumes, preferably 2 to 20 volumes and most preferably 3 to 10 volumes.
In another embodiment of the present invention, the rifaximin solution is added to an anti-solvent. This type of addition is known in the art as reverse addition.
The addition and precipitation temperature is from about −20° C. to about 55° C., preferably about 0° C. to about 45° C. and most preferably about 10 to about 35° C.
Significantly, these processes are robust and independent of any critical parameters and the form produced is completely amorphous. Surprisingly, this form is chemically and polymorphically stable on storage and is relatively unaffected by external parameters such as ambient humidity. For instance, exposure of the amorphous form of the present invention to ambient humidity for a period of 10 days did not change the polymorphic form as indicated by PXRD spectroscopy. High yield of amorphous form of rifaximin is isolated by the processes of the present invention and it has high chemical purity and retains acceptable amounts of residual solvents.
The following non-limiting examples further illustrate the manner of carrying out the inventive process described herein.
Crude rifaximin (50 g) was dissolved in ethyl acetate (150 mL) in a three necked flask under nitrogen and filtered off to remove insoluble material. Heptanes (150 mL) were added slowly at ambient temperature. After complete precipitation, the precipitated product was isolated by filtration and dried at ambient temperature in a vacuum oven to obtain 41 g (82%) of pure amorphous rifaximin. The PXRD diffractogram and DSC thermogram are essentially the same as depicted in
Rifaximin (25 g) was charged to a three necked flask along with 4 volumes of ethyl acetate under nitrogen and stirred to dissolve. This solution was clarified by filtration through a Buchner funnel and then added to 6 volumes of heptanes at room temperature. After complete precipitation, the product was isolated by Buchner filtration and dried in a vacuum oven at ambient temperature to provide 23 g (92%) of pure amorphous rifaximin. The PXRD diffractogram and DSC thermogram are essentially the same as depicted in
Rifaximin (10 g) was dissolved in acetone (35 mL) in a three necked flask under nitrogen. One volume was removed by distillation, and the hot solution was allowed to cool and filtered to remove insoluble material. This solution was added to heptanes (10 vol). After complete precipitation, the precipitated material was isolated by filtration and dried at ambient temperature in a vacuum oven to provide 5 g of amorphous rifaximin. The PXRD diffractogram and DSC thermogram are essentially the same as depicted in
Rifaximin (5 g) was charged into a three necked flask along with 2-butanol (50 mL) under nitrogen and the mixture was stirred to dissolve. The solution was added to heptanes (16 vol). After complete precipitation, the precipitated amorphous rifaximin was isolated by filtration and dried at ambient temperature in a vacuum oven to provide 4.1 g (82%) of amorphous rifaximin. The PXRD diffractogram and DSC thermogram are essentially the same as depicted in
Rifaximin (10 g) was dissolved in 5 volumes of methyl iso-butyl ketone in a three necked flask under nitrogen. The solution was clarified by Buchner filtration and the solution was added to 5 volumes heptanes. After complete precipitation, the product was isolated by filtration and dried at ambient temperature in a vacuum oven to provide 9 g (90%) amorphous rifaximin. The PXRD diffractogram and DSC thermogram are essentially the same as depicted in
Crude rifaximin (5 g) was charged to a three necked flask, followed by 4 volumes of ethyl acetate under nitrogen and the mixture was stirred to dissolve. The solution was clarified by filtration into a flask and this saturated solution was added to methyl tert-butyl ether (16 vol). After complete precipitation, it was filtered and the isolated product was dried in a vacuum oven at ambient temperature to obtain 2.4 g of amorphous rifaximin. The PXRD diffractogram and DSC thermogram are essentially the same as depicted in
While the foregoing provides a detailed description of the preferred embodiment and examples of the invention, it is to be understood that this description is illustrative only of the principles of the invention and not limitative. Furthermore, as many changes can be made to the invention without departing from the scope of the invention, it is intended that all material contained herein be interpreted as illustrative of the invention and not in a limiting sense.
