Claims
- 1. An analyte detector, comprising:
a first variable volume reagent container; a sample receiving cup in fluid communication with the first variable volume reagent container; a capture chamber in fluid communication with the first variable volume reagent container and the sample receiving cup; and a first antibody residing in the capture chamber, the first antibody being specific for an analyte of interest.
- 2. The analyte detector of claim 1, wherein the first variable volume reagent container is collapsible.
- 3. The analyte detector of claim 1, further comprising a first reagent transmission tube joined to the first variable volume reagent container and joined to the sample receiving cup.
- 4. The analyte detector of claim 3, wherein at least part of the first reagent transmission tube is a capillary tube.
- 5. The analyte detector of claim 3, wherein an end of the first reagent transmission tube is beveled to provide a penetrating edge proximate to the first variable volume reagent container.
- 6. The analyte detector of claim 1, further comprising a sample transmission tube joined to the sample receiving cup and joined to the capture chamber.
- 7. The analyte detector of claim 6, wherein at least part of the sample transmission tube is a capillary tube.
- 8. The analyte detector of claim 6, further comprising a first reagent transmission tube joined to the first variable volume reagent container and joined to the sample receiving cup, and further comprising a drain joining the sample cup to the first reagent transmission tube and the sample transmission tube.
- 9. The analyte detector of claim 1, further comprising a plug having a diameter approximately equal to a diameter of the drain.
- 10. The analyte detector of claim 1, further comprising a mixing chamber in fluid communication with the sample transmission tube.
- 11. The analyte detector of claim 10, further comprising a filter disposed in the mixing chamber.
- 12. The analyte detector of claim 1, further comprising a filter having an inlet in fluid communication with the sample cup and an outlet in fluid communication with the capture chamber.
- 13. The analyte detector of claim 1, further comprising a second variable volume reagent container in fluid communication with the capture chamber.
- 14. The analyte detector of claim 13, wherein the second variable volume reagent container is collapsible.
- 15. The analyte detector of claim 13, further comprising a second reagent transmission tube joined to the second variable volume reagent container and in fluid communication with the capture chamber.
- 16. The analyte detector of claim 15, wherein an end of the second reagent transmission tube is beveled to provide a penetrating edge proximate to the second variable volume reagent container.
- 17. The analyte detector of claim 1, further comprising a reservoir in fluid communication with the capture chamber.
- 18. The analyte detector of claim 17, further comprising an absorbent material disposed in the reservoir.
- 19. The analyte detector of claim 18, wherein the reservoir has an exit orifice.
- 20. The analyte detector of claim 19, further comprising a hydrophobic material disposed in the exit orifice.
- 21. The analyte detector of claim 1, wherein the first antibody is labeled.
- 22. The analyte detector of claim 1, wherein the first antibody is labeled with a label selected from the group consisting of a fluorescent label, a radiolabel, and a chromophore.
- 23. The analyte detector of claim 1, wherein the analyte of interest is a mammallian hormone.
- 24. The analyte detector of claim 23, wherein the mammal hormone is selected from the group consisting of luteinizing hormone, estradiol, follicle-stimulating hormone, progesterone, and combinations thereof.
- 25. The analyte detector of claim 1, wherein the first antibody is a monoclonal antibody.
- 26. The analyte detector of claim 1, wherein the first antibody is a polyclonal antibody.
- 27. The analyte detector of claim 1, wherein the first antibody is immobilized in the capture chamber.
- 28. The analyte detector of claim 1, wherein a second antibody is in the first variable volume reagent container.
- 29. The analyte detector of claim 1, further comprising a second antibody specific to a combination of the analyte of interest and the first antibody.
- 30. The analyte detector of claim 29, wherein the second antibody is labeled.
- 31. The analyte detector of claim 29, wherein the second antibody is labeled with a label selected from the group consisting of a fluorescent label, a radiolabel, and a chromophore.
- 32. The analyte detector of claim 29, wherein the second antibody is in the second variable volume reagent container.
- 33. An analyte detection method, comprising:
providing an analyte detector having a first variable volume reagent container with a first reagent therein, and having a sample receiving cup in fluid communication with the first variable volume reagent container, and having a capture chamber in fluid communication with the first variable volume reagent container and the sample receiving cup, and having a first antibody residing in the capture chamber, the first antibody being specific for an analyte of interest; providing a sample of body fluid in the sample receiving cup; reducing the volume of the first variable volume reagent container to force the first reagent toward the capture chamber; determining whether the first antibody combined with the analyte of interest.
- 34. The analyte detection method of claim 33, wherein the sample of body fluid is blood.
- 35. The analyte detection method of claim 33, wherein reducing the volume of the first variable volume reagent container includes compressing the first variable volume reagent container.
- 36. The analyte detection method of claim 33, further comprising piercing the first variable volume reagent container to release the first reagent from the first variable volume reagent container.
- 37. The analyte detection method of claim 33, further comprising providing a plug in the sample receiving cup.
- 38. The analyte detection method of claim 33, wherein determining whether the first antibody combined with the analyte of interest includes detecting electromagnetic radiation emanating from the capture chamber.
- 39. The analyte detection method of claim 33, wherein the analyte of interest is selected from the group consisting of luteinizing hormone, estradiol, follicle-stimulating hormone, progesterone, and combinations thereof.
- 40. The analyte detection method of claim 33, further comprising filtering the sample of body fluid.
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This is a continuation-in-part of U.S. patent application Ser. No. 09/408,215 (filed Sep. 29, 1999), and applicants claim priority to that patent application, and that patent application is incorporated herein by reference. Applicants also claim priority to two earlier filed U.S. provisional patent applications, Ser. No. 60/122,658 (filed Mar. 3, 1999) and No. 60/102,199 (filed Sep. 29, 1998), both of which are incorporated herein by reference.
Provisional Applications (2)
|
Number |
Date |
Country |
|
60122658 |
Mar 1999 |
US |
|
60102199 |
Sep 1998 |
US |
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
09408215 |
Sep 1999 |
US |
Child |
09817909 |
Mar 2001 |
US |