Patent Application
20110024296
1. Field of the Invention
The present invention is in the field of analytical electrophoresis systems and methods. The invention can include high resolution and highly sensitive Isotachophoresis (ITP) and capillary electrophoresis (CE) assays.
2. Description of the Related Art
Electrophoresis is generally the movement of charged molecules in an electric field. Analytical methods based on electrophoresis have found broad utility, especially in the fields of protein and nucleic acid analyses. Samples having charged analyte molecules of interest can be placed in a selective media, such as size exclusion media, ion exchange media, or media having a pH gradient, where they can differentially migrate for high resolution from other sample molecules. The separated molecules can be detected for identification and quantitation.
Capillary and microfluidic scale electrophoretic separations are particularly popular for analyses requiring low sample volumes or high throughput. For example, chips of plastic or glass substrate can be fabricated with microscale loading channels, separation channels and detection channels. Samples can be transferred from microwell plates through a robotically manipulated sample collection tube to the loading channel. An electric potential can induce movement of sample constituents through selective media in the separation channel for sequential detection as the constituents elute into the detection channel from the separation channel. The microscale dimensions of the assay system can provide rapid analyses using microscale, or nanoscale, sample volumes. However, resolution or sensitivity may not be adequate for complex samples or dilute samples.
One approach to enhancing the resolution and sensitivity of capillary electrophoresis (CE) methods has been to pre-resolve and pre-concentrate the sample using Isotachophoresis (ITP) before CE separations. In ITP, the sample is loaded into a channel between a leading electrolyte (LE) having an electrophoretic mobility greater than the sample and a trailing electrolyte (TE) having electrophoretic mobility less than the sample. Under the influence of an electric field, analytes of interest can migrate through the sample bolus to accumulate at the interface with the LE and/or TE solutions. In this way, the analytes of interest can be separated from certain other constituents of the sample and concentrate to more detectable levels. Samples can thus be concentrated and desalted to provide improved injection material for further capillary electrophoresis separations resulting in highly sensitive detections with high resolution. For example, in “Tandem Isotachophoresis-Zone Electrophoresis via Base-Mediated Destacking for Increased Detection Sensitivity in Microfluidic Systems”, by Vreeland, et al., Anal. Chem. (2003) ASAP Article, sample concentrated by ITP is further resolved and detected by capillary zone electrophoresis (CZE). In Vreeland, the sample is subjected to ITP between a TE and an LE having electrophoretic mobilities controlled by the pH of Tris buffers. While ITP concentration of analytes progresses, hydroxyl ions (—OH) are formed by hydrolysis at the cathode end of the separation channel. Migration of the hydroxyl ions through the separation channel eventually neutralizes the Tris buffers to remove the mobility differences between the LE and TE solutions. The Tris neutralization converts the ITP separation media into a CZE separation media. The analytes can than be separated with higher sensitivity and resolution than for standard CZE of the same sample due to the effective sample volume reduction and concentration of analytes resulting from the ITP assay step. The Vreeland method is limited to pH based ITP of compatible samples, can be time consuming due to the neutralization step, and can be inconsistent due to variations in buffer preparation or hydroxyl ion generation.
In another scheme to combine ITP with CE, analytes of interest migrate in ITP mode until they reach an intersection with a CE separation channel before switching the electric field to the separation channel for capillary electrophoresis separation of the analytes. For example, in “Sample Pre-concentration by Isotachophoresis in Microfluidic Devices”, by Wainright, et al., J. Chromat. A979 (2002), pp. 69-80, samples are pre-concentrated in a ITP channel until they reach an intersection with a CE channel. The intersection is monitored microscopically by a photomultiplier tube (PMT) receiving light through a confocal lens focused on the intersection. Analytes entering the intersection can be detected, e.g., by fluorescence or light absorption, and the electric field manually switched to inject the analytes into the CE channel. Problems exist, however, in that the manual switching can be inconsistent, some analytes may not be detectable using a PMT, and PMT detection at the microscale can be cumbersome and expensive.
In view of the above, a need exists for increased sensitivity, consistency, and resolution of capillary and microscale electrophoresis methods. It would be desirable to have systems that can automatically and consistently switch between electrophoretic modes. The present invention provides these and other features that will be apparent upon review of the following.
The present invention provides, e.g., systems and methods to consistently inject analytes into separation media based on a triggering voltage event. The analytes can be preconditioned and concentrated in a channel by isotachophoresis (ITP) stacking, followed by application of the stacked analytes to a separation channel segment, when a voltage event is detected in the channel.
The methods of the invention can provide highly repeatable analytical results with high sensitivity, speed and resolution. The method can include, e.g., analyte injection by stacking one or more analytes in a stacking channel segment, detecting a voltage potential in the channel, and applying the stacked analyte into a separation channel segment by applying an electric field or a pressure differential along the separation channel segment when a selected voltage event is detected. The channel can be a microscale channel, e.g., with intersecting or common channel segments making up a loading channel segment, a stacking channel segment and/or a separation channel segment.
Stacking of analytes can take place in a stacking channel segment wherein analytes of interest can be sandwiched between buffers selected to focus the analytes into a concentrated band during ITP. Typical injected analytes include, e.g., proteins, nucleic acids, carbohydrates, glycoproteins, ions, and/or the like. The stacking channel segment can have a trailing electrolyte and/or a leading electrolyte which have different mobilities. For example, the leading electrolyte can have a faster mobility under the influence of an electric field than the trailing electrolyte or analytes of interest. In many embodiments, the trailing electrolyte and the leading electrolyte can differ in pH, viscosity, conductivity, size exclusion, ionic strength, ion composition, temperature, and/or other parameters that can affect relative migration of the electrolytes. The trailing electrolyte can be adjusted to have a mobility less than analytes so that the analytes accumulate at the trailing interface during ITP. Optionally, the leading electrolyte can be adjusted to have a mobility greater than the one or more analytes so that they can accumulate at the leading interface during ITP separations. By narrowly adjusting the migration rates of trailing and leading electrolytes, analytes can be focused between the leading and trailing electrolytes while sample constituents not of interest migrate to other zones of the stacking channel segment. That is, the trailing electrolyte can be adjusted to have a mobility greater than one or more sample constituents not of interest, or the leading electrolyte can be adjusted to have a mobility less than one or more sample constituents not of interest so that they are not focused with the analyte of interest between the electrolytes.
When the channel of the analyte injection method includes separate stacking and separation channel segments, switching from the stacking channel to the separation channel segment can be by switching the electric field from the stacking channel segment to the separation channel segment, e.g., when the stacked analyte enters an intersection of the stacking and separation channel segments. For example, applying an electric field to the separation channel segment can include switching from a substantial lack of current in the separation channel segment while an electric current flows in the stacking channel segment to an electric current in the separation channel segment while electric current in the stacking channel segment is shut off. Shutting off (substantial lack) of current in the channel segments can be by application of a float voltage to prevent current flow in the channel segment or simply by provision of a high resistance in the channel segment (e.g., allowing no significant electric current outlet from the channel segment). Optionally, switching can be by exerting a pressure differential across the separation channel segment.
The separation channel segment in the injection methods can resolve analytes from other analytes or sample constituents. Such resolution can allow the analytes of interest to be identified or quantitated. Separation channel segments can have selective conditions or separation media to affect migration of analytes and sample constituents. For example, the separation channel can contain a pH gradient, size selective media, ion exchange media, a viscosity enhancing media, hydrophobic media, and/or the like.
Analytes resolved in separation channel segments can be detected for identification and/or quantitation. Detectors can be focused to monitor analytes in the separation channel segment or to detect analytes as they elute from the separation channel segment. Detecting analytes can be by monitoring parameters associated with the analytes, such as, e.g., conductivity, fluorescence, light absorbance, refractive index, and/or the like.
Sample solutions can be loaded to channels of the methods by a variety of techniques, e.g., to provide adequate sensitivity and speed. For example, when the loading channel does not hold enough sample analyte for the desired detection, multiple samples can be consecutively loaded and stacked before fusion of multiple stacks to provide an enhanced concentration of analyte in a small volume. Stacking two or more samples of the analytes can proceed by, e.g.: loading a first sample into a loading channel; applying an electric field across the sample, thereby stacking the sample; loading a second sample into the loading channel; and applying an electric field across the stacked sample and the second sample to stack the second sample and cause the two stacked samples to become focused together between trailing and leading electrolytes. The multiple stacking technique can be facilitated by flowing the stacked first sample towards the loading channel to clear excess electrolyte and depleted sample solution before loading the second sample. Another way to concentrate sample analytes can be, e.g., by loading samples of the analytes in a loading channel comprising a cross-section greater than a stacking channel segment cross-section so that analytes from a large sample volume do not have to migrate as far to accumulate at a trailing or leading electrolyte interface.
Spacer electrolytes, having migration rates intermediate to the trailing electrolyte and the leading electrolyte, can be loaded between samples and/or stacked analytes to resolve the sample into two or more analytes of interest. In one embodiment, stacking comprises loading one or more spacer electrolytes having a mobility greater than the trailing electrolyte and less than the leading electrolyte between two or more analyte sample segments. In another embodiment, one or more of the two or more analyte sample segments is a previously stacked sample analyte, and the spacer electrolyte is inserted during a multi-stacking load procedure. The spacer electrolytes can be adjusted to provide a mobility between mobilities of two or more of the analytes in order to resolve the analytes in ITP. Such spacer electrolyte adjustments can be made by selecting an appropriate electrolyte pH, spacer electrolyte constituents, spacer electrolyte viscosity, spacer electrolyte conductivity, and/or the like.
In some injection methods, electrolytes can be intelligently formulated to provide ITP resolution of analytes for injection. For example, if the pK of an analyte is determined, e.g., from experiments or calculations, leading and trailing electrolytes can be adjusted to pH values bracketing the pK so that analyte intruding into the leading electrolyte becomes less charged and less mobile, and/or analyte intruding into the trailing electrolyte becomes more charged and more mobile. Such adjustments can enhance the selectivity and concentration of ITP before injection of the stacked analyte.
The injection of stacked analyte into a separation channel segment can be triggered by detection of a selected voltage event. Voltages can be monitored at various locations in the channel and voltage events that precisely indicate preferred timing for injection can be determined. For example, detecting a voltage event can include monitoring a float voltage necessary to maintain a zero current flow (or other defined current flow) condition in the separation channel segment. Typical voltage events used to trigger the start of a separation can include, e.g., a voltage peak, a voltage trough, a predesignated voltage, relative voltage, or a rate of voltage change, (for example a zero slope observed at the top of a voltage profile). The switch to inject stacked analyte from ITP to the separation channel segment can be an automatic application of an electric field or pressure differential along the channel segment when the voltage event is detected.
Systems of the invention for injection of analytes can provide automated injection of stacked analytes for reliable, consistent, and sensitive analyses. Analyte injection systems can include, e.g., an analyte stacking in a channel, a voltage detector in electrical contact with the channel and in communication with a controller so that the controller can initiate a flow of electrical current in a separation segment of the channel, or a pressure differential along the channel segment, when a selected voltage event is detected by the voltage detector. Typically, the channel is a microscale channel having a loading channel segment, a stacking channel segment, and a separation channel segment.
A stacking channel segment in the system is usually configured for isotachophoresis procedures with a trailing electrolyte (TE) and/or a leading electrolyte (LE). The electrolytes can have different adjustable mobilities. For example, the electrolytes can have different pH values, viscosities, conductivities, size exclusion cut-offs, ionic strengths, ion compositions, or temperatures. Analytes for stacking in the channel can include molecules, such as proteins, nucleic acids, carbohydrates, glycoproteins, derivatized molecules, ions, and the like. Electrolytes can be tailored to selectively stack analytes of interest while rejecting other sample constituents. For example, the trailing electrolyte can be formulated to have a mobility less than the mobility of the analyte of interest and a mobility greater than a mobility of a sample constituent not of interest, so that the analyte accumulates on the front of the TE while the constituent falls away through the TE. The LE can be formulated to have a mobility greater than the mobility of the analyte of interest and a mobility less than a mobility of a sample constituent not of interest, so that the analyte can accumulate at the LE interface while the constituent migrates away in front of the LE interface.
The separation channel segment of the system can contain conditions or selective media to resolve analytes and constituents that have been stacked in the stacking column. For example, the separation column can include a pH gradient, size selective media, ion exchange media, hydrophobic media, viscosity enhancing media, and the like.
The controller can receive output from the voltage detector to initiate an injection when a selected voltage event is detected. The controller can be, e.g., a logic device or a system operator. In some embodiments, the injection event can be a switch from the stacking channel ITP electric field conditions to driving forces required to insert stacked analyte into a separation channel segment. For example, the injection can be a switch from the ITP current flow to substantial elimination of current in the stacking channel segment when the voltage event is detected, while a field or pressure is initiated in the separation channel segment.
The channel segments of the system can include a loading channel segment in fluid contact with the stacking channel segment. Various loading schemes can be employed to meet the demands of particular analyses. In one embodiment, the loading channel segment can have a cross-section greater than a stacking channel segment cross-section so that a larger volume of sample analyte can accumulate in the stacking channel segment in a shorter amount of time, i.e., the average analyte molecule has a shorter migration distance across a large cross-section loading channel segment than with a long loading channel segment of the same volume. In another aspect of loading, a first stacked analyte sample can be pulled back toward the loading channel segment before loading a second sample in a multiple stacking scheme to increase the analyte concentration and sensitivity of an assay. The “pull back” can be accomplished, e.g., by providing a pressure differential across the stacking channel segment to cause the first stacked sample to flow back toward the loading channel segment. Loading channel segments can be filled from, e.g., wells on a microfluidic chip, or by fluid handling systems, such as receiving samples from microarrays through a collector tube (sipper).
Spacer electrolytes can be used in the system, e.g., to enhance resolution between two or more analytes of interest. For example, a spacer electrolyte with a mobility between the mobilities of two or more analytes can be introduced between sample segments containing the analytes in the stacking channel segment. Analytes slower than the spacer electrolyte can partition behind the spacer while faster analytes can partition in front of the spacer. In an alternate embodiment, the sample analyte can be combined with spacer electrolytes, e.g., to partition into separate analyte zones, e.g., under the influence of transient or steady state conditions in ITP.
Systems of the invention can have voltage detectors in communication with controllers to detect and respond to voltage events in channels. Voltage detectors can detect voltages between two or more electric contacts across segments of channels, or between contacts at any location in the channel and a voltage reference, such as a ground. In some embodiments of the systems, the voltage detector monitors the voltage in the separation channel segment while stacking progresses. The voltage of the separation channel segment during stacking can be monitored at an intersection with the stacking channel segment or anywhere along the separation channel segment, e.g., when no substantial current flows in the separation channel segment, such as when a float voltage is being applied to the separation channel segment by a float voltage regulator, where there is no electrical outlet from one end of the channel segment, or where the channel segment has a controlling switch in the off position.
Controllers can automatically switch the system from stacking mode to separation mode on detection of a selected voltage event to inject stacked analytes into the separation channel segment. The voltage event can be, e.g., a voltage peak, a selected voltage, a voltage trough, a relative voltage, a rate of voltage change, and/or the like. The automatic switch can be, e.g., flowing of electrical current in the channel segment, a change in relative voltages across a channel segment, or application of a pressure differential along the channel segment to induce migration of the stacked analytes along the separation channel segment.
Analytes separated in the separation channel segments can be detected by analyte detectors of the system to identify and/or quantitate analytes of interest. Analyte detectors can be configured to monitor analytes in the separation channel segment, or analytes eluting from the separation channel segment. The analyte detector can comprise a fluorometer, a spectrophotometer, a refractometer, a conductivity meter, and/or the like.
The systems of the invention are well suited to microfluidic applications. For example, the loading channel segments, stacking channel segments, separation channel segments, detection chambers, and the like, can be incorporated into a microfluidic chip. The microscale dimensions of microfluidic devices are compatible with many systems of the invention. Microfluidic systems known in the art can provide voltages, pressures, fluid handling, communications, and detectors, etc., useful in practicing the systems of the present invention.
Unless otherwise defined herein or below in the remainder of the specification, all technical and scientific terms used herein have meanings commonly understood by those of ordinary skill in the art to which the present invention belongs.
Before describing the present invention in detail, it is to be understood that this invention is not limited to particular methods or systems, which can, of course, vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments only, and is not intended to be limiting.
As used in this specification and the appended claims, the singular forms “a”, “an” and “the” include plural referents unless the content clearly dictates otherwise. Thus, for example, reference to “a constituent” can include a combination of two or more constituents; reference to “the analytes” can include one analyte, and the like.
Although many methods and materials similar, modified, or equivalent to those described herein can be used in the practice of the present invention without undue experimentation, the preferred materials and methods are described herein. In describing and claiming the present invention, the following terminology will be used in accordance with the definitions set out below.
The term “stacking,” as used herein, refers to accumulation of one or more analytes at an interface with electrolytes having different mobilities in an electric field, e.g., during isotachophoresis. Stacking of analytes can also occur at other zones created, e.g., by migration modifying constituents present in the assay sample under transient (dynamic) or steady state conditions.
In “selective isotachophoresis” an analyte of interest with a known mobility can be separated from another sample constituent, such as, e.g., a sample constituent not of interest, e.g., by providing leading electrolyte and/or trailing electrolytes with mobilities intermediate to the mobilities of the analyte and sample constituent.
The term “analyte,” as used herein, refers to constituents of a sample that are detected by an analyte detector. An “analyte of interest”, as used herein, refers to an analyte for which detection and/or quantitation is desired in an assay.
The term “channel,” as used herein, refers to a conduit for flowing and/or retention of fluids in methods and systems of the invention. Channels can be, e.g., tubes, columns, capillaries, microfluidic channels, and/or the like. A channel can include various channel segments, e.g., in separate sections of the channel, that share sections of the channel, and/or that intersect with other segments of the channel. Channel segments are generally functional sections of channel, such as, e.g., loading channel segments, stacking channel segments, and separation channel segments.
A “skewing channel” in the invention can be a channel segment that causes skewing of sample constituents flowing in the channel. For example, the internal surface topography of a skewing channel can cause bands or peaks to take on an oblique orientation relative to the channel axis while passing through the skewing channel.
The term “mobility,” as used herein, refers to a rate of migration for charged molecules, such as analytes or electrolytes, in a solution under the influence of an electric field in a channel.
The term “float voltage,” as used herein, refers to a voltage required in a channel segment to substantially prevent flow of an electric current through the segment or to establish a desired constant current in the segment.
The term “microscale,” as used herein, refers to dimensions ranging from about 1000 μm to about 0.1 μm.
The invention relates to methods and systems for injection of analytes into separation channels. Stacking sample analytes can provide higher analyte concentrations in smaller injection volumes for electrophoretic separations with improved assay sensitivity and resolution. Sensitivity and separations can be improved, in many cases, by stacking analytes in skewing channels before injection. Automated timing of injections triggered by detection of voltage events can improve the consistency of results between assay runs.
Methods and systems of the invention can be used to separate, identify, and/or quantify analytes with a high level of sensitivity and resolution. Analytes of the invention can be, e.g., charged molecules, such as, e.g., proteins, nucleic acids, carbohydrates, glycoproteins, ions, derivatized molecules, and/or the like.
Methods of the invention can provide precise injection timing of stacked analyte into a separation channel for sensitive, repeatable, high resolution assays. Methods of the invention generally include, e.g., loading a sample to a loading channel segment before isotachophoresis (ITP) in a stacking channel segment, detecting a voltage event that indicates a stacked sample analyte is in position for injection, applying an electric field or pressure differential to apply the stacked sample analyte to a separation channel segment, and detecting separated analytes of interest. The ITP can include migration of the analytes through skewing channels. Detection signals can be evaluated to determine the presence or quantity of the analytes.
Analytes of interest can be stacked into a volume less than the original analyte sample by isotachophoresis (ITP). For example, a sample bolus can be loaded between two different buffer systems in a channel and exposed to an electric current to create a steady state of solute zones migrating in order of decreasing mobility. In the steady state, the zones can adopt the same concentration and migrate along the channel at the same velocity as the leading electrolyte. Alternatively, a sample bolus can be loaded adjacent to an electrolyte and stacked in a dynamic (transient) condition at the interface for injection, e.g., without having reached a steady state equilibrium between ITP electrolytes.
Stacking can be practiced, e.g., in channels of a microfluidic chip wherein a sample is loaded between channel regions of a trailing electrolyte and a leading electrolyte. As shown in
Stacking can also be practiced in a transient state. For example, as shown in
Multiple analytes of interest can accumulate in a steady state or transient state, e.g., at one or both of the electrolyte interfaces. For example, as shown in
In methods of the invention, the mobilities of trailing electrolytes and leading electrolytes can be adjusted to provide selective pre-concentration of an analyte of interest while separating sample constituents not of interest from the analyte. For example, as shown in
Trailing electrolytes and leading electrolytes can be tailored, according to methods known in the art, by adjusting electrolyte mobilities to provide highly specific retention and stacking of analytes of interest, while sample constituents not of interest are removed. In one embodiment of the methods, the pH of electrolytes is selected to bracket the pK of an analyte of interest so that sample constituents not of interest having pKs outside the bracket will be removed in the ITP. The pK of the analytes of interest can be determined, e.g., empirically or based on the known molecular structure of the analytes. In other embodiments, the analyte of interest can be, e.g., closely bracketed between selected trailing and leading electrolyte compositions known to have slower and faster mobilities than the analyte. Many ions and buffers can be used in electrolytes to bracket analytes, such as, e.g., chloride, TAPS, MOPS, and HEPES. Optionally, the mobility of electrolytes and/or analytes can be modulated by adjusting the viscosity or size exclusion characteristics of the sample solution, trailing electrolyte solution, and/or leading electrolyte solution. In another option for adjusting the mobility of ITP solutions, mobility of analyte solutions and/or electrolyte solutions can be moderated, particularly during transient ITP migrations, by adjusting the concentration, ionic strength, or conductivity of the solutions. The temperature of solutions can be selected in still other options to adjust the mobility of analytes, electrolytes, or ITP solutions.
A variety of sample solution loading methods can benefit analyses in methods of the invention. Stacking channels can be loaded with single sample solution loads, with multiple sample solution loads, and with spacer electrolyte between sample solution loads, as described in detail below.
Single sample loads can be loaded to sample loading channel segments according to techniques known in the art, e.g., as shown in
Additional amounts of sample solution can be loaded for ITP using a multiple stacking technique. A first sample can be loaded into loading channel segment 60 as shown in
Optionally, a large volume of sample solution can be loaded into a loading channel segment having a cross-section greater than the cross-section of the stacking channel segment. As shown in
Advantages can be obtained in methods of the invention by placing a spacer electrolyte between analyte sample segments for ITP. The spacer electrolyte can have a mobility intermediate between the trailing electrolyte and the leading electrolyte. The spacer electrolyte can have a mobility intermediate between two or more analytes of interest. The spacer electrolyte can provide, e.g., enhanced resolution between multiple analytes of interest. In one embodiment, spacer electrolyte can be present in loaded sample solutions to provide a spacer zone between analytes on application of an electric field. In another embodiment, spacer electrolyte can be loaded between cycles of multiple stacking. For example, multiple stacking can proceed as described above, but with spacer electrolyte present to the left of the initial stack, with spacer electrolyte present in one or more loaded sample solution segments, or by loading spacer electrolyte between cycles of loading sample solution segments. Spacer electrolytes can be adjusted as described above for adjustment of trailing and leading electrolyte mobilities to tailor spacer migration between analytes of interest.
Detection of voltage events associated with, e.g., migration of solutions, analytes, and/or electrolytes in the stacking channel segment can provide, e.g., a consistent signal for initiation of stacked analyte application to a separation channel segment. During an ITP, voltage potentials across the stacking channel segment, or voltages measurable at any point along the stacking channel segment, can vary with time. From one ITP run to the next, there can be measurable voltage events that are consistent between runs and which can act as timing markers useful for consistent triggering of injections and the switch from an ITP to a different separation scheme.
In a typical embodiment of detecting a voltage event, trailing electrolyte, analyte, and leading electrolyte are flowing in a stacking channel segment during an ITP. The trailing electrolyte has a higher resistance to electric current flow than the leading electrolyte. With a voltmeter monitoring voltage, e.g., at a point half way along the stacking channel segment, as shown in
A separation channel segment in electrical contact with a stacking channel segment will have no substantial flow of electric current if the separation channel is not part of a complete circuit (e.g., a “dead end” with no ground connection) or if a float voltage is applied to the separation channel segment. In a preferred configuration for detecting voltage events, the voltmeter contact can be located at a point between the separation channel segment and the stacking channel segment, or at any location along the separation channel segment. In one preferred embodiment, voltage events can be detected by monitoring a separation channel segment float voltage.
Separation of analytes of interest from other sample constituents can be enhanced by stacking the analyte during and/or after passage through a skewing channel segment. For example, sensitivity of an assay can be increased when sample constituents not of interest become dispersed by the turns while the analyte of interest continues to be focused by electrolytes in the isotachophoresis method.
Analyte bands flowing in channels of an analytical system can become dispersed when the channel diverges from a straight path. For example, as shown in
In one embodiment, for example, a small amount of analyte of interest can be separated from a larger amount of sample constituent not of interest with an enhanced degree of sensitivity and improved quantitation. In an ITP system without skewing channels, as shown schematically for example in
The benefits of ITP separations in skewing channels can be increased by selecting trailing and/or leading electrolytes to enhance the stacking focus of the analyte while increasing the mobility difference between the electrolytes and the sample constituent. In selective ITP the mobilities of leading and trailing electrolytes are selected, e.g., to be near the known mobility of an analyte and/or to increase the difference in mobility between the electrolytes and one or more sample constituents not of interest. For example, in the situation described above, where the analyte of interest has a mobility greater than the sample constituent not of interest, the trailing electrolyte can be selected to have a mobility closer to the analyte than to the sample constituent so that, e.g., the analyte is closely shepherded while the sample constituent lags behind to experience the effects of skewing and diffusion. In a similar fashion, if the analyte of interest has a mobility less than the sample constituent not of interest, the mobility of the leading electrolyte can be selected to be between the analyte mobility and the sample constituent mobility to enhance skewing channel ITP separation. In a preferred embodiment, the mobility of an electrolyte is selected to be between the mobilities of the analyte of interest and one or more sample constituents not of interest but closer to the mobility of the analyte. In another example, both leading and trailing electrolytes can be selected to be close to the known mobility of the analyte of interest. This can provide particular benefits when both faster and slower sample constituents migrate near the analyte and/or when transient stacking prevails during the ITP.
The effectiveness of skewing channel ITP can vary widely depending on factors, such as, e.g., the radius of any turns involved, the internal diameter of the channel, the topography of the channel walls, the cross section of the skewing channel, the flow velocity, and the viscosity of solutions. For example, as is discussed in the Skewing Channel ITP Systems section below, skewing in a channel can be increased with short turn radii, repeated turns in the same direction, channel topographies that increase the difference between the surface length of opposite channel walls, and channel cross sections that are wider perpendicular to the axis of a turn. Appropriate conditions for a particular method or system can be derived, e.g., through calculation and/or experimentation.
To consider how diffusion can affect the amount of skew caused by a turn, a two-dimensional, nondimensionalized advection-diffusion equation can be considered (see also, Analytical Chemistry, vol 73, No. 6, 1350-1360, Mar. 15, 2001):
wherein L is the length of the turning channel, w is the internal width of the turning channel, and Pe′w, is the dispersion Peclet number; u′, c′, t′, x′ and y′ are the normalized velocity, concentration, time, axial channel dimension, and transverse channel dimension, respectively. Three parameters, Pe′w, L, and w, have been determined to be of special importance to skewing and dispersion of analytes under the influence of skewing channels in the present invention.
The Peclet number (Pe) is a dimensionless factor representing a ratio of advection (or forward movement) and diffusion of an analyte. If Pe is large, peaks skewed by passage through a first skewing channel can retain a stable oblique shape long enough to have it reversed by a second turn in the opposite direction. If Pe is small, peaks skewed in a skewing channel can diffuse across the width of the channel in a relatively short time to convert a skewed peak into a diffusely broadened peak. In methods of the invention, sample constituents not of interest can be most readily skewed and dispersed from analytes of interest, e.g., when conditions exist in skewing channels providing a Peclet number more than about the ratio of the length of the skewing channel over the internal width of the skewing channel (i.e., Pe>L/w). Significant benefits in skewing, diffusion, and dispersion of sample constituents not of interest in skewing channel ITP can be obtained where conditions provide a Peclet number more than about 0.01 times, 0.1 times, 1 time, 10 times, 100 times, or more, than the ratio of the skewing channel length over the skewing channel width.
Conditions affecting the Peclet number can be, e.g., conditions that influence advection and/or diffusion of molecules in the channels, as is known by those skilled in the art. For example, Pe can be influenced by the viscosity of solutions, the presence of a gel, temperature, molecular concentrations, the velocity of the molecule along the channel, the diameter of the channel, and/or the like. Adjustment of conditions controlling advection and diffusion can provide Peclet numbers, e.g., that result in desirable levels of sample constituent dispersion during and/or after passage through skewing channel segments of the invention.
Analytes stacked by ITP can be injected into a separation channel segment, e.g., by applying an electric field or pressure differential across the separation channel segment and the stacked analytes. The field and/or pressure can cause migration or flow of analytes into the separation channel segment. Application of the field or pressure can be triggered by detection of a voltage event, as described above, to provide consistent and functional analyte injection timing. Application of the separation channel segment electric field or pressure differential can coincide with elimination of current flow in the stacking channel segment. The timing between the voltage event and the injection can be established to conform to particular configurations of channels, intersections, and solution segments. The timing can also play a key role in determining the peak resolution and signal strength as it can affect the amount of transient isotachophoresis that persists after the handoff.
Separation channel segments can provide conditions for electrophoretic separation of analytes and/or separation by selective media. In preferred embodiments, separation channel segments have a microscale dimension (e.g., a depth or width ranging from about 1000 μm to about 0.1 μm, or from about 100 μm to about 1 μm), e.g., to provide fast separations of small analyte sample volumes. Separation channel segments can have separation media, such as, e.g., a pH gradient, size selective media, ion exchange media, a viscosity enhancing media, hydrophobic media, and/or the like, capable of contributing to the resolution of analytes. Separation channel segments (as well as stacking channel segments) can have viscosity enhancing media, such as gels, to reduce electroosmotic flow (EOF) in separation modes where EOF is undesirable. Separation channel segments can be independent from other channel segments, or can share all or part of a channel with other channel segments, such as, e.g., loading channel segments and stacking channel segments. In a preferred embodiment, the separation channel segment is independent, but intersects in a fluid contact at some point along the length of the stacking channel segment.
In a typical embodiment, stacked analyte from an ITP separation is injected into a separation channel segment when a peak voltage is detected at the intersection of a stacking channel segment and the separation channel segment. For example, the float voltage in separation channel segment 110 reaches a maximum (and the rate of voltage change, or slope of the voltage profile, becomes zero) as stacked analytes 111, sandwiched between trailing electrolyte 112 and leading electrolyte 113, migrate in an ITP past a voltmeter contact at the intersection of the separation channel segment with the stacking channel segment, as shown in
Alternate schemes for timing of injection will be appreciated by those skilled in the art. Such alternate schemes can be based, e.g., on calculations or models, or can be determined empirically. For example, time delays can be built into triggered responses based on channel volumes, channel geometry, voltmeter contact location, choice of voltage events, the location of analytes relative to solution features affecting voltage events, and/or the like. In a particular example, wherein analyte is stacked near a trailing electrolyte interface in a transient ITP (not yet reaching a steady state) and the remaining sample solution bolus has a high electrical resistance, a suitable trigger time can be a certain time after the voltage peak to allow the stacked analyte additional migration time to reach the intersection with the separation channel segment.
Application of an electric field along the separation channel segment can be automatic (that is, not requiring manual switching). Such automatic application of the electric field can be accomplished, e.g., by electronic devices and algorithms known in the art. For example, a voltmeter can be set to trip a switch when voltage at a contact reaches a set level. In preferred embodiments, a logic device, such as, e.g., an integrated circuit or a computer, can be programmed to initiate switching of actuators according to preset parameters (e.g., the occurrence of defined voltage events).
Analytes separated in by methods of the invention can be detected in the separation channel segment and/or sequentially after elution from the separation channel segment. Appropriate detectors can, e.g., be fixed to monitor analytes in a detection channel, sequentially scan for analytes in channel segments, or provide continuous imaging of entire channels.
Appropriate detectors are often determined by the type of analyte to be detected. Proteins and nucleic acids, for example, can often be detected by spectrophotometric monitoring of particular light absorption wavelengths. Many ionic analytes of interest can be detected by monitoring changes in solution conductivity. Many analytes are fluorescent or can be labeled with fluorescent markers for detection using a fluorometer. Many analytes in solution, particularly carbohydrates, can be detected by refractometry.
In a typical embodiment, detecting can be by monitoring transmission of a light source through a separation channel segment using a photomultiplier tube (PMT) focused on the channel with a microscope lens. Those skilled in the art will appreciate how such an arrangement can be configured as a fluorescence detector by addition of an appropriate excitation light source, such as, e.g., a laser or filtered light from a lamp. Optionally, the lens can be mounted on an X-Y scanning mechanism to monitor any location on a microfluidic chip. With such an arrangement, the length of a separation channel segment can be scanned for analytes, e.g., resolved along a pH gradient. In another embodiment, conductivity meter sensors can be mounted across a separation channel outlet to monitor charged analytes as they elute from the channel segment.
Detectors can be in communication with data storage devices and/or logic devices to document assay runs. Analog output from detectors, such as PMTs and conductivity meters, can be fed to chart plotters to retain a trace of the analyte separation profile on paper. Analog to digital converters can communicate detection signals to logic devices for data storage, separation profile presentation, and/or assay evaluation. Digital logic devices can greatly facilitate quantitation of analytes by comparison to appropriate standard curves from regression analysis.
Electrokinetic analyte injection systems described herein can provide sensitive analyte detection with high resolution in a highly consistent manner. Analytes selectively stacked in a stacking channel segment can be injected (applied) into a separation channel segment with precise timing based on detection of voltage events in the channels. Such precision can be enhanced by provision of automated injection subsystems.
Systems of the invention generally include, e.g., an analyte stacking in a channel, a voltage detector in communication with a controller and in contact with the channel at one or more locations, an electric current or pressure differential established in the channel when a selected voltage event is detected by the voltage detector and communicated to the controller. The channel can include stacking channel segments and separation channel segments that intersect, form a continuous channel or which share common channel sections. Analytes applied to the separation channel segment and separated can be, e.g., detected by a detector in communication with a logic device to determine the presence of particular analytes or to evaluate the quantity of analytes.
The channel of the invention can be, e.g., a single multifunction channel comprising loading segments, stacking segments, separation segments, and/or detection segments. Optionally, the channel can include separate loading channel segments, stacking channel segments, and separation channel segments in fluid contact at intersections. In a preferred embodiment, as shown schematically in
Channels of a microfluidic device can be embedded on the surface of a substrate by mold injection, photolithography, etching, laser ablation, and the like. The channels can have a microscale dimension, such as, e.g., a depth or width ranging from about 1000 μm to about 0.1 μm, or from about 100 μm to about 1 μm. Fluids can flow in the channels, e.g., by electroosmotic flow, capillary action (surface tension), pressure differentials, gravity, and/or the like. Channels can terminate, e.g., in wells of solutions and/or at intersections with other channels or chambers. Channels can have electrical contacts, e.g., at each end to provide electric fields and/or electric currents to separate analytes or to induce EOF. Detectors can be functionally associated with channels to monitor parameters of interest, such as, e.g., voltages, conductivity, resistance, capacitance, electric currents, refractivity, light absorbance, fluorescence, pressures, flow rates, and/or the like. Microfluidic chips can have functional information communication connections and utility connections to supporting instrumentation, such as electric power connections, vacuum sources, pneumatic pressure sources, hydraulic pressure sources, analog and digital communication lines, optic fibers, etc.
The channel can include, e.g., a load channel segment to introduce one or more sample solution volumes into the channel. Such loading channels can be configured in ways appreciated by those skilled in the art, such as, e.g., as an injector loop, to include an a collector tube 120 to a microfluidic chip, as shown in
Channels of the systems can contain gelatinous substances to beneficially affect migration and flow characteristics of the channels. Gels can be incorporated into channels to reduce unwanted electroosmotic flows of solutions while providing a more electrophoretic character to a separation. Gels can influence the relative migration rates of analytes and/or electrolytes by slowing the progress of larger molecules. Gels can provide tools to help adjust migration zones for analytes and ITP electrolytes in stacking channel segments. For example, analytes of interest are generally larger than commonly used ITP electrolytes. By placing a gel in a stacking channel segment, a fast analyte (large but with a high charge to mass ratio) can be slowed to migrate behind a leading electrolyte small molecule salt or buffer. Optionally, a gel can slow an analyte to migrate only marginally faster than a trailing electrolyte. Gel resistance to large molecule migration can be adjustable, e.g., by altering the type of gel, concentration of gel matrix, and the extent of gel matrix cross-linking. Gels can provide enhanced concentration and/or resolution to analytes in stacking or separation channel segments. One or more different gels can be present in either the stacking channel segment or the separation channel segment.
Stacking channel segments can function to selectively stack analytes of interest, e.g., by ITP, for injection into a separation channel segment for further resolution and detection. Stacking channel segments can have electrical contacts, e.g., at each end, for application of electric fields suitable for analyte stacking. Stacking channel segments can have fluid contacts with, e.g., externally driven pneumatic or hydraulic manifolds so that pressure driven flows, such as electrolyte loading or the pull back for the multiple stacking technique discussed in the “Stacking Analytes of Interest” section above, can be practiced. Stacking channel segments can contain, e.g., electrolytes, such as trailing electrolytes, spacer electrolytes, and/or leading electrolytes, suitable for isotachophoresis (ITP), as discussed in the Methods section above. The stacking channel segment can have trailing electrolyte well 18, as shown in
Separation channel segments can receive stacked analytes by injection from stacking channel segments for further resolution by separation techniques, such as, e.g., additional rounds of ITP, ion exchange, size exclusion, hydrophobic interaction, reverse phase chromatography, isoelectric focusing, capillary zone electrophoresis, and/or the like. Separation channel segments can include electric contacts for application of electric fields along the channel segment and/or external connections with pressure sources to drive fluid flows. Separation channel segments can be, e.g., a channel segment intersecting a stacking channel segment, a channel segment continuing a common channel with a stacking channel segment, and/or a channel segment functionally sharing channel sections with a stacking channel segment. In a typical embodiment, the separation channel segment intersects the stacking channel segment at some point along the stacking channel segment length, as shown in
Isotachophoresis systems of the invention can include skewing channel segments in, and/or before, the stacking channel to enhance the separation of analytes of interest from sample constituents not of interest. The sample constituents can be dispersed while the analyte of interest is focused by stacking, e.g., in the skewing channels. The separation enhancement can be promoted, e.g., by turning through cumulatively large angles, sharp turning, skewing channel cross sections having relatively large widths, skewing channel topographies with opposite surfaces of different length, and/or skewing channel systems having conditions providing a Peclet number more than about the ratio of the skewing channel length over the skewing channel width.
One way to increase skew and dispersion in skewing channel segments is to provide greater turning angles in the channel. In a two dimensional plane, turning angles can be accumulated, e.g., with continuous spiral turns or switching serpentine turns as shown in
Skew and dispersion from passage through skewing channel segments can be more pronounced in channels that make sharp turns relative to the internal channel diameter. For example, skew is increased for skewing channels with a high ratio of channel internal diameter over turn width. In one embodiment, the skew from a skewing channel segment having turns is increased when the cross-section of the channel is greater along the radius of the turn (skewing channel internal width) than perpendicular to the turn radius (skewing channel depth).
The topography of a skewing channel segment can affect the skew and dispersion of migrating analytes. For example, channel surface contours that increase the ratio between the travel distance along the outside of a turn over the travel distance along the inside of the turn can increase skew. Skew can be increased by increasing channel internal width relative to channel depth at turn points. As shown in
Selective stacking of the analyte of interest between leading and trailing electrolytes is an important aspect of skewing channel ITP systems of the invention. Analytes of interest can be continuously refocused between the electrolytes during and/or after skewing while sample constituents not of interest become dispersed. The mobility of an analyte of interest can be known from calculations or by empirical data. The trailing and/or leading electrolyte can be selected to have a mobility between those of the analyte of interest and intrusive sample constituents not of interest. To enhance focusing of the analyte and dispersion of sample constituents, the electrolytes can be selected to have mobilities closer to that of the analyte of interest than the sample constituents.
Skewing ITP channel segments can be incorporated into the systems and methods of injecting analytes described above. An analyte of interest can be injected into a separation channel at higher purity after dispersion of other sample constituents by skewing channel ITP. Injection of the analyte can be initiated on detection of a voltage event.
Voltage detectors in systems of the invention can be in contact with channels to detect voltage events communicated to a controller. The type and complexity of voltage detectors can depend on, e.g., channel hardware configurations and the type of voltage event to be detected.
Voltage detectors can range from, e.g., simple relay switches tripped by a voltage, to analog galvanometers, to analog devices with chart recorders, to voltmeters with digital outputs for evaluation by logic devices. Voltmeters generally detect a voltage potential between electrodes at two locations, such as, e.g., a contact location in a channel and a ground, or between two different locations in a channel. The location of the voltage electrode contacts with the channel can change the voltage profile detected during a stacking run. However, a well defined voltage event can often be determined for consistent and unambiguous triggering of an injection for voltmeter contacts at a wide range of channel locations (e.g., the voltmeter contact does not have to be at an intersection between stacking and separation channel segments).
In one embodiment, voltmeter contacts can be located at two ends of the channel. As trailing electrolyte, of relatively high resistance, displaces leading electrolyte in the channel, the voltage required to maintain a selected current through the channel can increase. A voltage event to trigger injection in this case can be, e.g., a preset voltage.
In another embodiment, voltmeter contacts can be located at a ground (or other voltage reference) and at any point in a separation channel segment intersecting a stacking channel segment. If electric current is not allowed to flow through the separation channel segment (e.g., where the separation channel segment is held at zero current by a float voltage, or where the separation channel segment not part of a complete circuit), any location in the separation channel segment will reflect the stacking channel segment voltage at the intersection. Voltage detected in the separation channel segment can rise to a peak and fall as the TE/LE interface passes the intersection, in a fashion similar to the voltage profile of
Where voltage is being monitored in a separation channel segment without electrical current and in contact with the stacking channel segment, the lack of current can be by, e.g., float voltage regulation or circuit isolation. A float voltage regulator device can be an electronic device, known in the art, that detects electric current flow in a channel segment and applies a voltage to the channel segment that neutralizes any voltage potential across the channel segment, thus preventing a flow of electric current. A float voltage regulator can optionally be configured to adjust a channel segment voltage differential to provide a selected constant current in the channel segment. Another way to prevent electric current flow in a channel segment is to ensure that the channel segment is not a part of a completed electric circuit. For example, an electric switch can be present at one end of the channel segment to selectively open or close any associated electric circuits.
The voltmeter can communicate with a controller for initiation of analyte application (injection) to a separation channel segment. Initiation of injection can be manual or automatic. For example, the voltmeter can provide a visible voltage readout for a system operator (the controller) to manually switch channel electric fields or fluid flows on observation of a voltage event, such as a selected voltage or voltage peak. In another example, the controller is a digital logic device in electronic communication with the voltmeter and set to automatically apply stacked analytes to a separation channel segment on detection of a selected voltage event.
Appropriate analyte detectors can be incorporated into systems of the invention to detect analytes. The type and configuration of detectors can depend, e.g., on the type of analyte to be detected and/or on the layout of channels. Analyte detectors can be in communication with logic devices for storage of analyte detection profiles and evaluation of analytical results.
Analytes for detection in the systems can range widely, with many being charged molecules or molecules modified to have a charge. For example, analytes of interest can be proteins, nucleic acids, carbohydrates, glycoproteins, ions, and/or the like. Although stacking can take place by alternate mechanisms, such as size exclusion, stacking is driven by migration of charged analytes in an electric field for many systems of the invention. It will be appreciated by those skilled in the art that non charged analytes of interest can receive a charge for electrophoretic stacking by appropriate adjustment of pH or derivatization of the analyte with a charged chemical group.
Analyte detectors in the systems can be any suitable detectors known in the art. For example, the detectors can be fluorometers, spectrophotometers, refractometers, conductivity meters, and/or the like. Analytes not detectable by available detectors can often be derivatized with a marker molecule to render then detectable. The detectors can be mounted or focused to monitor analytes in the channel segments, including, e.g., intersections and/or separation channel segments. Detectors can monitor analytes as they exit separation channel segments, e.g., in detection channels of chambers.
Analyte detectors can monitor a channel location, sequentially scan a channel length, or provide a continuous image of separated analytes. In one embodiment, a stationary spectrophotometric detector can be a photomultiplier tube focused on a particular channel location or intersection. In another embodiment, the analyte detector can be a fluorometer focused on microchannels through a confocal microscope lens mounted to an X-Y transporter mechanism to sequentially scan analytes separated in channels of a microfluidic device. In another embodiment, the analyte detector can be a charge coupled device (CCD) array capable of providing an image of numerous separations in multiple separation chambers at once.
The analyte detector can be in communication with a logic device for storage and evaluation of analytical results. Logic devices of the systems can include, e.g., chart recorders, transistors, circuit boards, integrated circuits, central processing units, computer monitors, computer systems, computer networks, and/or the like. Computer systems can include, e.g., digital computer hardware with data sets and instruction sets entered into a software system. The computer can be in communication with the detector for evaluation of the presence, identity, quantity, and/or location of an analyte. The computer can be, e.g., a PC (Intel x86 or Pentium chip—compatible with DOS®, 052®, WINDOWS® operating systems) a MACINTOSH®, Power PC, or SUN® work station (compatible with a LINUX or UNIX operating system) or other commercially available computer which is known to one of skill. Software for interpretation of sensor signals or to monitor detection signals is available, or can easily be constructed by one of skill using a standard programming language such as Visualbasic, Fortran, Basic, Java, or the like. A computer logic system can, e.g., receive input from system operators designating sample identifications and initiating analysis, command robotic systems to transfer the samples to the loading channel segments of the system, control fluid handling systems, control detector monitoring, receive detector signals, prepare regression curves from standard sample results, determine analyte quantity, and/or store analytical results.
It is understood that the examples and embodiments described herein are for illustrative purposes only and that various modifications or changes in light thereof will be suggested to persons skilled in the art and are to be included within the spirit and purview of this application and scope of the appended claims.
While the foregoing invention has been described in some detail for purposes of clarity and understanding, it will be clear to one skilled in the art from a reading of this disclosure that various changes in form and detail can be made without departing from the true scope of the invention. For example, many of the techniques and apparatus described above can be used in various combinations.
All publications, patents, patent applications, and/or other documents cited in this application are incorporated by reference in their entirety for all purposes to the same extent as if each individual publication, patent, patent application, and/or other document were individually indicated to be incorporated by reference for all purposes.
This application is a divisional of U.S. patent application Ser. No. 10/936,159, filed Sep. 7, 2004, and claims the benefit of U.S. Provisional Application No. 60/500,387, filed Sep. 5, 2003, and U.S. Provisional Application No. 60/518,169, filed Nov. 7, 2003.
| Number | Date | Country | |
|---|---|---|---|
| 60500387 | Sep 2003 | US | |
| 60518169 | Nov 2003 | US |
| Number | Date | Country | |
|---|---|---|---|
| Parent | 10936159 | Sep 2004 | US |
| Child | 12902006 | US |
