Claims
- 1. A device for analyte detection, comprising:
an analyte-shaped cavity; and an enhancing structure associated with said analyte-shaped cavity.
- 2. The device of claim 1, further comprising an analyte molecule associated with said analyte-shaped cavity.
- 3. The device of claim 2, wherein said enhancing structure is sufficiently close to said analyte to enhance a Raman signal produced by said analyte.
- 4. The device of claim 1, wherein said analyte-shaped cavity is a virus-shaped cavity.
- 5. The device of claim 4, further comprising at least two virus-shaped cavities, and wherein one cavity of said at least two cavities is complementary to a first portion of said virus, and a second cavity of said at least two cavities is complementary to a second portion of said virus.
- 6. A biochip comprising:
a substrate an analyte-shaped cavity; and at least one enhancing structure associated with said analyte-shaped cavity.
- 7. The biochip of claim 6, comprising a plurality of analyte-shaped cavities.
- 8. A biochip comprising:
a substrate; an array comprising a plurality of defined areas on said substrate, each of said defined areas having at least one analyte-shaped cavity thereon; and at least one enhancing structure associated with said cavity.
- 9. The biochip of claim 8, wherein at least one of said analyte-shaped cavity is complementary to a virus.
- 10. The biochip of claim 6, wherein said at least analyte-shaped cavity is complementary to at least one virus.
- 11. A system for analyte detection, comprising:
a substrate having a plurality of defined areas thereon, each of said areas having:
at least one enhancing structure thereon; and at least one analyte-shaped cavity; wherein each of said plurality of analyte-shaped cavities associated with each of said defined areas has a preferential analyte binding affinity different from the binding affinities of analyte-shaped cavities associated with different defined areas; an identifier for at least one of said defined areas; and a detector associated with a defined area.
- 12. The system of claim 11, further comprising a computer for storing data and for data analysis.
- 13. A method for analyte detection, comprising:
providing an enhancing structure; providing an analyte-shaped cavity near said enhancing structure; exposing said analyte to said analyte-shaped cavity to form an analyte analyte-shaped cavity complex; and detecting a Raman spectral feature associated with said analyte receptor complex.
- 14. The method of claim 13, further comprising the steps of:
analyzing said spectral feature; and comparing said spectral feature with a known reference spectral feature.
- 15. A method for manufacturing a matrix for analyte detection, comprising the steps of:
providing an analyte within a polymerizable matrix; adding to said polymerizable matrix a catalyst and a plurality of enhancing structures; permitting said polymerizable matrix to polymerize forming a polymer matrix; removing said analytes from said polymer matrix.
- 16. A method of manufacturing a matrix for analyte detection, comprising the steps of:
providing a substrate having a surface and an analyte molecule attached thereto; providing a polymerizable matrix having a plurality of enhancing structures therein; overlaying said polymerizable matrix onto said substrate and said analyte molecule; and permitting said polymerizable matrix to polymerize forming a polymer matrix having analyte-shaped cavities on one surface of said matrix.
- 17. A method for manufacturing a biochip; comprising the steps of:
providing a substrate having at least one defined area thereon; providing a plurality of enhancing structures; providing the polymer matrix of claim 15.
- 18. A method for manufacturing a biochip, comprising the steps of:
providing a substrate having at least one defined area thereon; applying the polymer matrix of claim 16 to said at least one defined area, so that the surface of said matrix having said analyte-shaped cavities is exposed.
- 19. A Raman reader comprising:
a light source; a matrix array having enhancing structures and analyte-shaped cavities thereon; a holder for positioning said matrix array in relation to said light source; a light detector.
- 20. The reader of claim 19, further comprising a computer for comparing detected light with known spectral features of known moieties.
- 21. The reader of claim 20, further comprising a memory device for storing a program for analyzing said spectral features.
- 22. The reader of claim 21, further comprising a device for acquiring information from an external database.
- 23. A method for detecting an analyte on a biochip, comprising the steps of:
providing a biochip having at least one defined area thereon having at least one analyte-shaped cavity thereon; providing a Raman reader; exposing said biochip to a solution containing an analyte; permitting said analyte to be retained by said analyte-shaped cavity forming an analyte analyte-shaped cavity complex; exposing said defined area to incident light; and collecting light emitted from a defined area.
RELATED APPLICATIONS
[0001] This application claims priority to U.S. Provisional Patent Application Serial No. 60/356,254, filed Feb. 11, 2002, and is a continuation-in-part of U.S. Applications Serial Nos. 10/298,725 filed Nov. 18, 2002, which is a continuation-in-part of U.S. Ser. No. 10/294,385 filed Nov. 14, 2002, which is a continuation-in-part of U.S. Ser. No. 09/925,189 filed Aug. 8, 2001, which is a continuation-in-part of U.S. Ser. No. 09/815,909 filed Mar. 23, 2001, which is a continuation-in-part of U.S. Ser. No. 09/670,4453, which claimed priority to U.S. Provisional Patent Application Serial No. 60/156,195 filed Sep. 27, 1999, now abandoned, to U.S. Serial No. 60/156,145 filed Sep. 27, 1999, now abandoned, and to No. 60/156,471 filed Sep. 27, 1999, now abandoned. Each of the above patent applications is herein incorporated fully by reference.
Provisional Applications (4)
|
Number |
Date |
Country |
|
60356254 |
Feb 2002 |
US |
|
60156195 |
Sep 1999 |
US |
|
60156471 |
Sep 1999 |
US |
|
60156145 |
Sep 1999 |
US |
Continuation in Parts (5)
|
Number |
Date |
Country |
Parent |
10298725 |
Nov 2002 |
US |
Child |
10364160 |
Feb 2003 |
US |
Parent |
10294385 |
Nov 2002 |
US |
Child |
10364160 |
Feb 2003 |
US |
Parent |
09925189 |
Aug 2001 |
US |
Child |
10364160 |
Feb 2003 |
US |
Parent |
09815909 |
Mar 2001 |
US |
Child |
10364160 |
Feb 2003 |
US |
Parent |
09670453 |
Sep 2000 |
US |
Child |
10364160 |
Feb 2003 |
US |