Claims
- 1. An analytical element for measuring a specific component (X) in a fluid sample by means of competing reactions between a reaction of said component (X) with a material (R) which is capable of being specifically bound to said component (X) or an analogue (AX) of said component (X), and a reaction of a labeled-material (LX), which is formed from binding said specific component (X) or the analogue thereof (AX) with a labeling material (L), with said material (R), said analytical element comprising
- a first layer containing said material (R) immobilized therein and, a second layer containing a material (A) which is capable of at least reducing a signal caused by said labeling material (L) in said labeled-material (LX) by specifically binding with a labeling portion of said labeled-material (LX), said first and said second layers being porous.
- 2. The analytical element of claim 1, wherein said material (A) is contained in said second layer in an immobilized state.
- 3. The analytical element of claim 2, wherein said material (R) contained in said first layer is an antigen which is capable of specifically binding with an antibody.
- 4. The analytical element of claim 2, wherein said material (R) contained in said first layer is an antibody which is capable of specifically binding with an antigen.
- 5. The analytical element of claim 4, wherein said labeling material (L) is an enzyme and said material (A) contained in said second layer is an enzyme inhibitor which is capable of specifically binding with said enzyme and of reducing an activity of said enzyme.
- 6. The analytical element of claim 2, further comprising a layer containing said labeled-material (LX).
- 7. The analytical element of claim 6, further comprising a layer containing a color-forming reagent which forms a color with an enzyme.
- 8. The analytical element of claim 2, further comprising a layer containing the labeled-material (LX) and a color-forming reagent.
- 9. The analytical element of claim 2, further comprising a timing layer provided between the first layer and the second layer.
- 10. The analytical element of claim 1, wherein said material (R) contained in said first layer is an antigen which is capable of specifically binding with an antibody.
- 11. The analytical element of claim 1, wherein said material (R) contained in said first layer is an antibody which is capable of specifically binding with an antigen.
- 12. The analytical element of claim 1, wherein said material (A) contained in said second layer is an enzyme inhibitor which is capable of specifically binding with an enzyme (being used as the labeling material (L) and of reducing an activity of said enzyme.
- 13. The analytical element of claim 1, wherein said material (A) is contained in said second layer is capable of specifically binding with the labeling portion of the labeled-material (LX) and of reducing a fluorecence as a signal caused by a fluorecent substance (being used as the labeling material (L)).
- 14. The analytical element of claim 1, wherein said element further comprises a layer containing said labeled-material (LX).
- 15. The analytical element of claim 1, wherein said element further comprises a layer containing a color-forming reagent which forms a color with enzyme material.
- 16. The analytical element of claim 1, wherein said element further comprises a layer containing the labeled-material (LX) and a color-forming reagent.
- 17. The analytical element of claim 1, 14, 15 or 16, wherein said element further comprises a timing layer provided between the first layer and the second layer.
- 18. A method for the measurement of a specific component (X) in a fluid sample by means of competing reactions between a reaction of said component (X) with a material (R) which is capable of being specifically bound with said component (X) or an analogue of said component (AX), and a reaction of a labeled-material (LX), which is formed from binding said component (x) or the analogue thereof (Ax) with a labeling material (L), with said material (R), said method comprising,
- providing said fluid sample and said labeled-material (LX) into an analytical element comprising as its structural member; a first layer containing said material (R) immobilized therein and, a second layer containing a material (A) which is capable of at least reducing a signal caused by said labeling material (L) in said labeled-material (LX) by specifically binding with a labeling portion of said labeled-material (LX), said first and said second layers being porous; and measuring strength of signal caused by said labeling material (L).
Priority Claims (1)
Number |
Date |
Country |
Kind |
60-131955 |
Jun 1985 |
JPX |
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Parent Case Info
This application is a continuation of application Ser. No. 874,504, filed June 16, 1986 now abandoned.
US Referenced Citations (17)
Continuations (1)
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Number |
Date |
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Parent |
874504 |
Jun 1986 |
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