Patent Application
20190177667
Disclosed are compositions with anti-adherent properties and methods of inhibiting the adherence of microbes to a surface. More specifically, disclosed are compositions that include an anti-adherent agent that inhibits the adherence of microbes to a surface and methods of using such compositions. The microbes may be at least one of DNA viruses, RNA viruses, Gram negative bacteria, or Gram positive bacteria. The composition may be applied to or incorporated into articles such as wipes, or into ointments, lotions, creams, salves, aerosols, gels, suspensions, sprays, foams, washes, or the like.
Communicable human infections pass from person to person through various means such as food, aerosols, surfaces and hands. For example, in the United States, foodborne pathogens alone cause an estimated 76 million cases of illness, 325,000 hospitalizations and 5,000 deaths per year. This results in the spending or loss of several billion dollars due to absenteeism, cost of medication, and hospitalization.
Foodborne pathogens are typically a result of poor cleaning of hands and surfaces on which food is prepared. In fact, the kitchen is one of the most contaminated sites in the home. High fecal and coliform concentrations can be found in sponges, dishcloths, and the kitchen sink. Of course, there are other pathogens lurking elsewhere in the home, at the office, and in public places such as public bathrooms, restaurants, malls, theaters, health-care facilities, etc. Such pathogens include bacteria, protein, active enzymes, viruses, and many other microbes that can lead to health problems.
DNA viruses, including the adenovirus, are among these pathogens that can be spread and lead to health problems, such as the common cold, sore throats, pneumonia, and diarrhea. Other DNA viruses include herpes viruses, such as HSV-1, HSV-2, cytomegalovirus, and Epstein-Barr. Other DNA viruses can lead to diseases such as smallpox and chickenpox. RNA viruses, including influenza, noroviruses, rhinoviruses, polio virus, and enteroviruses, are also common causes of diseases in humans. These viruses can lead to symptoms of vomiting, diarrhea, body aches, and fevers, among others. DNA and RNA viruses, like other pathogens, can be commonly spread by shaking hands with infected people or touching a surface or object with such viruses on it.
Gram negative bacteria can include Escherichia coli (E. coli) and other Gram negative rods (e.g., Entereobacteria, Salmonella choleraesuis, Klebsiella pneumoniae, Pseudomonas aeruginosa); Gram negative curved rods (e.g., Vibrio, Heliobacter, Campylobacter, etc.); and Gram negative cocci (e.g., Neisseria). Gram negative bacteria can cause diarrhea, urinary tract infections, respiratory illnesses and pneumonia, and other illnesses. Gram positive bacteria can include Staphylococcus aureus (S. aureus). Other Gram positive bacteria include Gram positive rods (e.g., Bacillus, Clostridium, Listeria, etc.) and Gram positive cocci (e.g., Staphylococcus, Streptococcus, etc.). Gram positive bacteria can cause skin infections, pneumonia, and meningitis, among other illnesses. Gram negative and Gram positive bacteria can be commonly spread by shaking hands with infected people or by touching a surface or object with such bacteria on it.
There are products used today that are used to clean skin and hard surfaces where pathogens may be deposited, such as soaps, hand sanitizers, sprays and wipes. However, even the most diligent efforts to keep clean can be hindered by factors such as surface topography, the presence of hair, and the like. These factors can cause pathogens to better adhere to a surface. Other limiting factors include skin sensitivity due to the handling of cleaning products or the application thereof.
There remains a need for methods of reducing the adherence of microbes to a surface as well as compositions for accomplishing the same. The anti-adherent composition can be applied to surfaces or incorporated into articles, wherein the compositions inhibit the adherence of microbes. Desirably, the compositions are skin friendly, cost effective, and convenient to use.
In one aspect of the disclosure, a method of inhibiting microbes from attaching to a surface can include providing an anti-adherent composition. The anti-adherent composition can include an anti-adherent agent configured to inhibit microbes from attaching to the surface. The anti-adherent agent can be selected from the group consisting of: Acrylates Copolymer, Trimethylpentanediol/Adipic Acid/Glycerin Crosspolymer, Trimethylpentanediol/Adipic Acid copolymer, Ethylhexyl Stearate, Ethylhexyl Salicylate, Acrylates/C12-22 Alkylmethacrylate Copolymer, Octocrylene, Ethylene Oxide/Propylene Oxide Block Copolymer, and Polyquaternium-101, and any combinations thereof. The method can further include applying the composition to the surface. The method can also include allowing at least some of the composition to remain on the surface such that the anti-adherent agent inhibits microbes from attaching to the surface.
In another aspect of the disclosure, an anti-adherent composition for preventing microbes from attaching to a surface can include an anti-adherent agent and a humectant. The anti-adherent agent can be selected from the group consisting of: Trimethylpentanediol/Adipic Acid/Glycerin Crosspolymer, Trimethylpentanediol/Adipic Acid copolymer, Ethylhexyl Stearate, and Acrylates/C12-22 Alkylmethacrylate Copolymer, and any combinations thereof.
In yet another aspect of the disclosure, an anti-adherent composition for preventing microbes from attaching to a surface can include an anti-adherent agent and a humectant. The anti-adherent agent can be selected from the group consisting of: Acrylates Copolymer, Trimethylpentanediol/Adipic Acid/Glycerin Crosspolymer, Trimethylpentanediol/Adipic Acid copolymer, Ethylhexyl Stearate, Ethylhexyl Salicylate, Acrylates/C12-22 Alkylmethacrylate Copolymer, and Polyquaternium-101, and any combinations thereof. The anti-adherent composition can be non-antimicrobial.
The present disclosure is directed to a method of inhibiting the adherence of microbes to a surface and an anti-adherent composition containing an anti-adherent agent and a carrier that inhibits the adherence of microbes to a surface. The composition can be applied to a surface in the form of a liquid, gel, or foam; or incorporated into a wash. In addition, the composition can be applied to a surface with a vehicle such as a wipe.
The anti-adherent composition may be used on biotic surfaces such as skin or plants; or abiotic surfaces such as food prep surfaces; hospital and clinic surfaces; household surfaces; automotive, train, ship and aircraft surfaces; and the like; as long as the surface is compatible with the ingredients of the composition. Applying the anti-adherent composition to such surfaces can help prevent the amount or the likelihood that microbes will adhere to those surfaces, thus, lessening the likelihood of further transferring of the microbes.
Importantly, preferred embodiments of the anti-adherent composition of the present disclosure are not antimicrobial (“non-antimicrobial”). In other words, in preferred embodiments the anti-adherent composition does not include an effective amount of any antimicrobial agent(s). For purposes of this disclosure, an effective amount of an antimicrobial agent can be considered as such a concentration such that a kill of a desired microbe reaches the IC50 (inhibitory concentration 50%) for that desired microbe with that antimicrobial agent. The IC50 is defined as the amount of antimicrobial agent that is able to reduce the population of a selected microbe by 50% at a defined contact time and incubation temperature. In some embodiments, the anti-adherent composition can be free of antimicrobial agents. In these preferred embodiments in which the anti-adherent composition is non-antimicrobial, the anti-adherent composition seeks to prevent attachment of microbes to a surface, not eradicate or inactivate the microbes. This distinction can provide a benefit for the effectiveness for preventing the further spreading of microbes as concerns grow about the increasing microbial resistance to common antimicrobial treatments. However, in some alternative embodiments, as will be discussed further below, it is contemplated that the anti-adherent composition can include antimicrobial agents at an effective amount such that the anti-adherent composition is antimicrobial.
Anti-adherent agents suitable for use in the composition can inhibit microbes from attaching to a surface. Suitable anti-adherent agents can include Acrylates Copolymer, Trimethylpentanediol/Adipic Acid/Glycerin Crosspolymer, Trimethylpentanediol/Adipic Acid Copolymer, Ethylhexyl Stearate, Ethylhexyl Salicylate, Acrylates/C12-22 Alkylmethacrylate Copolymer, Octocrylene, Ethylene Oxide/Propylene Oxide Block Copolymer, and Polyquaternium-101. The anti-adherent agents can be non-antimicrobial.
An exemplary Acrylates Copolymer can be Carbopol® Aqua SF-1 Polymer available from The Lubrizol Corporation. An exemplary Trimethylpentanediol/Adipic Acid/Glycerin Crosspolymer can be Lexorez® 200 available from Inolex. An exemplary Trimethylpentanediol/Adipic Acid Copolymer can be Lexorez® TL-8 available from Inolex. An exemplary Ethylhexyl Stearate can be Cetiol® 868 available from BASF Corporation. An exemplary Ethylhexyl Salicylate can be Escalol® 587 available from Ashland Inc. An exemplary Acrylates/C12-22 Alkylmethacrylate Copolymer can be Allianz OPT available from Ashland Inc. An exemplary Octocrylene can be Escalol® 597 available from Ashland Inc. An exemplary Ethylene Oxide/Propylene Oxide Block Copolymer can be Pluronic® 85 available from BASF Corporation. An exemplary Polyquaternium-101 can be Deposilk Q1 available from Air Products and Chemicals, Inc.
Some embodiments of the anti-adherent compositions of the present disclosure can be suitably made with an anti-adherent agent in an amount of from about 0.01% (by the total weight of the composition) to about 20% (by total weight of the composition), or preferably from about 0.05% (by total weight of the composition) to about 15% (by total weight of the composition), or more preferably from about 0.1% (by total weight of the composition) to about 10% (by total weight of the composition). In some preferred embodiments, the anti-adherent composition can include an anti-adherent agent in an amount from about 2.0% (by the total weight of the composition) to about 5.0% (by the total weight of the composition). However, it is contemplated that anti-adherent compositions of the present disclosure can be outside of these ranges. For example, in some embodiments, the anti-adherent composition can include an anti-adherent agent that provides 100% of the total weight of the anti-adherent composition.
The anti-adherent compositions of the present disclosure may be formulated with one or more conventional and compatible carrier materials. The anti-adherent composition may take a variety of forms including, without limitation, aqueous solutions, gels, balms, lotions, suspensions, creams, milks, salves, ointments, sprays, emulsions, oils, resins, foams, solid sticks, aerosols, and the like. Liquid carrier materials suitable for use in the instant disclosure include those well-known for use in the cosmetic and medical arts as a basis for ointments, lotions, creams, salves, aerosols, gels, suspensions, sprays, foams, washes, and the like, and may be used in their established levels.
Non-limiting examples of suitable carrier materials include water, emollients, humectants, polyols, surfactants, esters, perflurocarbons, silicones, and other pharmaceutically acceptable carrier materials. In one embodiment, the carrier is volatile, allowing for immediate deposition of the anti-adherent ingredient to the desired surface while improving overall usage experience of the product by reducing drying time. Non-limiting examples of these volatile carriers include 5 cst Dimethicone, Cyclomethicone, Methyl Perfluoroisobutyl Ether, Methyl Perfluorobutyl Ether, Ethyl Perfluoroisobutyl Ether and Ethyl Perfluorobutyl Ether. Unlike conventional volatile carriers such as ethanol or isopropyl alcohol, these carriers have no antimicrobial effect.
In one embodiment, the anti-adherent compositions can optionally include one or more emollients, which typically act to soften, soothe, and otherwise lubricate and/or moisturize the skin. Suitable emollients that can be incorporated into the compositions include oils such as alkyl dimethicones, alkyl methicones, alkyldimethicone copolyols, phenyl silicones, alkyl trimethylsilanes, dimethicone, dimethicone crosspolymers, cyclomethicone, lanolin and its derivatives, fatty esters, glycerol esters and derivatives, propylene glycol esters and derivatives, alkoxylated carboxylic acids, alkoxylated alcohols, fatty alcohols, and combinations thereof.
Some embodiments of the anti-adherent compositions may include one or more emollients in an amount of from about 0.01% (by total weight of the composition) to about 20% (by total weight of the composition), or from about 0.05% (by total weight of the composition) to about 10% (by total weight of the composition), or from about 0.10% (by total weight of the composition) to about 5% (by total weight of the composition).
In another embodiment the anti-adherent compositions include one or more esters. The esters may be selected from cetyl palmitate, stearyl palmitate, cetyl stearate, isopropyl laurate, isopropyl myristate, isopropyl palmitate, and combinations thereof. The fatty alcohols include octyldodecanol, lauryl, myristyl, cetyl, stearyl, behenyl alcohol, and combinations thereof. Ethers such as eucalyptol, ceteraryl glucoside, dimethyl isosorbic polyglyceryl-3 cetyl ether, polyglyceryl-3 decyltetradecanol, propylene glycol myristyl ether, and combinations thereof can also suitably be used as emollients. Other suitable ester compounds for use in the anti-adherent compositions or the present disclosure are listed in the International Cosmetic Ingredient Dictionary and Handbook, 11th Edition, CTFA, (January, 2006) ISBN-10: 1882621360, ISBN-13: 978-1882621361, and in the 2007 Cosmetic Bench Reference, Allured Pub. Corporation (Jul. 15, 2007) ISBN-10: 1932633278, ISBN-13: 978-1932633276, both of which are incorporated by reference herein to the extent they are consistent herewith.
Humectants that are suitable as carriers in the anti-adherent compositions of the present disclosure include, for example, glycerin, glycerin derivatives, hyaluronic acid, hyaluronic acid derivatives, betaine, betaine derivatives amino acids, amino acid derivatives, glycosaminoglycans, glycols, polyols, sugars, sugar alcohols, hydrogenated starch hydrolysates, hydroxy acids, hydroxy acid derivatives, salts of PCA and the like, and combinations thereof. Specific examples of suitable humectants include honey, sorbitol, hyaluronic acid, sodium hyaluronate, betaine, lactic acid, citric acid, sodium citrate, glycolic acid, sodium glycolate, sodium lactate, urea, propylene glycol, butylene glycol, pentylene glycol, ethoxydiglycol, methyl gluceth-10, methyl gluceth-20, polyethylene glycols (as listed in the International Cosmetic Ingredient Dictionary and Handbook such as PEG-2 through PEG 10), propanediol, xylitol, maltitol, or combinations thereof. Humectants are beneficial in that they prevent or reduce the chance that the anti -adherent film, formed after the anti-adherent agent is applied to a surface, will crack.
The anti-adherent compositions of the disclosure may include one or more humectants in an amount of about 0.01% (by total weight of the composition) to about 20% (by total weight of the composition), or about 0.05% (by total weight of the composition) to about 10% by total weight of the composition), or about 0.1% (by total weight of the composition) to about 5.0% (by total weight of the composition).
The anti-adherent compositions may include water. For instance, where the anti-adherent composition is a wetting composition, such as described below for use with a wet wipe, the composition will typically include water. The anti-adherent compositions can suitably comprise water in an amount of from about 0.01% (by total weight of the composition) to about 99.98% (by total weight of the composition), or from about 1.00% (by total weight of the composition) to about 99.98% (by total weight of the composition), or from about 50.00% (by total weight of the composition) to about 99.98% (by total weight of the composition), or from about 75.00% (by total weight of the composition) to about 99.98% (by total weight of the composition).
In an embodiment where the anti-adherent composition serves as a wash (e.g. shampoo; surface cleaner; or hand, face, or body wash), the anti-adherent composition will include one or more surfactants. These may be selected from anionic, cationic, nonionic, zwitterionic, and amphoteric surfactants. Amounts may range from 0.1 to 30%, or from 1 to 20%, or from 3 to 15% by total weight of the composition.
Suitable anionic surfactants include, but are not limited to, C8 to C22 alkane sulfates, ether sulfates and sulfonates. Among the suitable sulfonates are primary C8 to C22 alkane sulfonate, primary C8 to C22 alkane disulfonate, C8 to C22 alkene sulfonate, C8 to C22 hydroxyalkane sulfonate or alkyl glyceryl ether sulfonate. Specific examples of anionic surfactants include ammonium lauryl sulfate, ammonium laureth sulfate, triethylamine lauryl sulfate, triethylamine laureth sulfate, triethanolamine lauryl sulfate, triethanolamine laureth sulfate, monoethanolamine lauryl sulfate, monoethanolamine laureth sulfate, diethanolamine lauryl sulfate, diethanolamine laureth sulfate, lauric monoglyceride sodium sulfate, sodium lauryl sulfate, sodium laureth sulfate, potassium laureth sulfate, sodium lauryl sarcosinate, sodium lauroyl sarcosinate, potassium lauryl sulfate, sodium trideceth sulfate, sodium methyl lauroyl taurate, sodium lauroyl isethionate, sodium laureth sulfosuccinate, sodium lauroyl sulfosuccinate, sodium tridecyl benzene sulfonate, sodium dodecyl benzene sulfonate, sodium lauryl amphoacetate and mixtures thereof. Other anionic surfactants include the C8 to C22 acyl glycinate salts. Suitable glycinate salts include sodium cocoylglycinate, potassium cocoylglycinate, sodium lauroylglycinate, potassium lauroylglycinate, sodium myristoylglycinate, potassium myristoylglycinate, sodium palmitoylglycinate, potassium palmitoylglycinate, sodium stearoylglycinate, potassium stearoylglycinate, ammonium cocoylglycinate and mixtures thereof. Cationic counter-ions to form the salt of the glycinate may be selected from sodium, potassium, ammonium, alkanolammonium and mixtures of these cations.
Suitable cationic surfactants include, but are not limited to alkyl dimethylamines, alkyl amidopropylamines, alkyl imidazoline derivatives, quaternised amine ethoxylates, and quaternary ammonium compounds.
Suitable nonionic surfactants include, but are not limited to, alcohols, acids, amides or alkyl phenols reacted with alkylene oxides, especially ethylene oxide either alone or with propylene oxide. Specific nonionics are C6 to C22 alkyl phenols-ethylene oxide condensates, the condensation products of C8 to C13 aliphatic primary or secondary linear or branched alcohols with ethylene oxide, and products made by condensation of ethylene oxide with the reaction products of propylene oxide and ethylenediamine. Other nonionics include long chain tertiary amine oxides, long chain tertiary phosphine oxides and dialkyl sulphoxides, alkyl polysaccharides, amine oxides, block copolymers, castor oil ethoxylates, ceto-oleyl alcohol ethoxylates, ceto-stearyl alcohol ethoxylates, decyl alcohol ethoxylates, dinonyl phenol ethoxylates, dodecyl phenol ethoxylates, end-capped ethoxylates, ether amine derivatives, ethoxylated alkanolamides, ethylene glycol esters, fatty acid alkanolamides, fatty alcohol alkoxylates, lauryl alcohol ethoxylates, mono-branched alcohol ethoxylates, natural alcohol ethoxylates, nonyl phenol ethoxylates, octyl phenol ethoxylates, oleyl amine ethoxylates, random copolymer alkoxylates, sorbitan ester ethoxylates, stearic acid ethoxylates, stearyl amine ethoxylates, synthetic alcohol ethoxylates, tall oil fatty acid ethoxylates, tallow amine ethoxylates and trid tridecanol ethoxylates.
Suitable zwitterionic surfactants include, for example, alkyl amine oxides, silicone amine oxides, and combinations thereof. Specific examples of suitable zwitterionic surfactants include, for example, 4-[N,N-di(2-hydroxyethyl)-N-octadecylammonio]-butane-1-carboxylate, S-[S-3-hydroxypropyl-S -hexadecylsulfonio]-3-hydroxypentane-1-sulfate, 3-[P,P-diethyl-P-3,6,9-trioxatetradexopcylphosphonio]-2-hydroxypropane-1-phosphate, 3-[N,N-dipropyl-N-3-dodecoxy-2-hydroxypropylammonio]-propane-1-phosphonate, 3-(N,N-dimethyl-N-hexadecylammonio)propane-1-sulfonate, 3-(N,N-dimethyl-N -hexadecylammonio)-2-hydroxypropane-1-sulfonate, 4-[N,N-di(2-hydroxyethyl)-N-(2-hydroxydodecyl)ammonio]-butane-1-carboxylate, 3-[S-ethyl-S-dodeoxy-2-hydroxypropyl(sulfonio]-propane-1-phosphonate, 3-[P,P-dimethyl-P-dodecylphosphonio]-propane-1-phosphonate, 5-[N,N-di(3-hydroxypropyl)-N-hexadecylammonio]-2-hydroxy-pentane-1-sulfate, and combinations thereof.
Suitable amphoteric surfactants include, but are not limited to, derivatives of aliphatic quaternary ammonium, phosphonium, and sulfonium compounds, in which the aliphatic radicals can be straight or branched chain, and wherein one of the aliphatic substituents contains from about 8 to about 18 carbon atoms and one substituent contains an anionic group, e.g., carboxy, sulfonate, sulfate, phosphate, or phosphonate. Illustrative amnphoterics are coco dimethyl carboxymethyl betaine, cocoamidopropyl betaine, cocobetaine, oleyl betaine, cetyl dimethyl carboxymethyl betaine, lauryl bis-(2-hydroxyethyl) carboxymethyl betaine, stearyl bis-(2-hydroxypropyl) carboxymethyl betaine, oleyl dimethyl gamma -carboxypropyl betaine, lauryl bis-(2-hydroxypropyl)alpha-carboxyethyl betaine, cocoamphoacetates, and combinations thereof. The sulfobetaines may include stearyl dimethyl sulfopropyl betaine, lauryl dimethyl sulfoethyl betaine, lauryl bis-(2-hydroxyethyl) sulfopropyl betaine and combinations thereof.
Optionally, one or more rheology modifiers, such as thickeners, may be added to the anti-adherent compositions. Suitable rheology modifiers are compatible with the anti-adherent agent. As used herein, “compatible” refers to a compound that, when mixed with the anti-adherent agent, does not adversely affect the anti-adherent properties of same.
A thickening system is used in the anti-adherent compositions to adjust the viscosity and stability of the compositions. Specifically, thickening systems prevent the composition from running off of the hands or body during dispensing and use of the composition. When the anti-adherent composition is used with a wipe product, a thicker formulation can be used to prevent the composition from migrating from the wipe substrate.
The thickening system should be compatible with the compounds used in the present disclosure; that is, the thickening system, when used in combination with the anti-adherent compounds, should not precipitate out, form a coacervate, or prevent a user from perceiving the conditioning benefit (or other desired benefit) to be gained from the composition. The thickening system may include a thickener which can provide both the thickening effect desired from the thickening system and a conditioning effect to the user's skin.
Thickeners may include, cellulosics, gums, acrylates, starches and various polymers. Suitable examples include are not limited to hydroxethyl cellulose, xanthan gum, guar gum, potato starch, and corn starch. In some embodiments, PEG-150 stearate, PEG-150 distearate, PEG-175 diisostearate, polyglyceryl-10 behenate/eicosadioate, disteareth-100 IPDI, polyacrylamidomethylpropane sulfonic acid, butylated PVP, and combinations thereof may be suitable.
While the viscosity of the compositions will typically depend on the thickener used and the other components of the compositions, the thickeners of the compositions suitably provide for a composition having a viscosity in the range of greater than 10 cP to about 30,000 cP or more. In another embodiment, the thickeners provide compositions having a viscosity of from about 100 cP to about 20,000 cP. In yet another embodiment, thickeners provide compositions having a viscosity of from about 200 cP to about 15,000 cP.
Typically, the anti-adherent compositions of the present disclosure include the thickening system in an amount of no more than about 20% (by total weight of the composition), or from about 0.01% (by total weight of the composition) to about 20% (by total weight of the composition). In another aspect the thickening system is present in the anti-adherent composition in an amount of from about 0.10% (by total weight of the composition) to about 10% (by total weight of the composition), or from about 0.25% (by total weight of the composition) to about 5% (by total weight of the composition), or from about 0.5% (by total weight of the composition) to about 2% (by total weight of the composition).
In one embodiment, the anti-adherent compositions can be delivered as a foam. In accordance with the present disclosure, in order to make the composition foamable, the composition is combined with a foaming agent such as at least one derivatized dimethicone.
The foaming agent is capable of causing the compositions to foam when the compositions are combined with air using, for instance, a manual pump dispenser. Although the anti-adherent compositions may be dispensed from an aerosol container, an aerosol is not needed in order to cause the compositions to foam. Also of particular advantage, the compositions are foamable without having to include fluorinated surfactants.
Various different derivatized dimethicone foaming agents may be used in the compositions of the present disclosure. The derivatized dimethicone, for instance, may comprise a dimethicone copolyol, such as an ethoxylated dimethicone. In one embodiment, the derivatized dimethicone is linear, although branched dimethicones may be used.
The amount of foaming agent present in the foaming compositions can depend upon various factors and the desired result. In general, the foaming agent can be present in an amount from about 0.01% to about 10% (by total weight of the composition), or from about 0.1% to about 5% (by total weight of the composition), or from about 0.1% to about 2% (by total weight of the composition).
When an anti-adherent composition is made foamable, it may be contained in an aerosol container. In an aerosol container, the composition is maintained under pressure sufficient to cause foam formation when dispensed.
In one embodiment, the anti-adherent compositions may include hydrophobic and hydrophilic ingredients, such as a lotion or cream. Generally, these emulsions have a dispersed phase and a continuous phase, and are generally formed with the addition of a surfactant or a combination of surfactants with varying hydrophilic/lipopiliclipophilic balances (HLB). Suitable emulsifiers include surfactants having HLB values from 0 to 20, or from 2 to 18. Suitable non-limiting examples include Ceteareth-20, Cetearyl Glucoside, Ceteth-10, Ceteth-2, Ceteth-20, Cocamide MEA, Glyceryl Laurate, Glyceryl Stearate, PEG-100 Stearate, Glyceryl Stearate, Glyceryl Stearate SE, Glycol Distearate, Glycol Stearate, lsosteareth-20, Laureth-23, Laureth-4, Lecithinm, Methyl Glucose Sesquistearate, Oleth-10, Oleth-2, Oleth-20, PEG-100 Stearate, PEG-20 Almond Glycerides, PEG-20 Methyl Glucose Sesquistearate, PEG-25 Hydrogenated Castor Oil, PEG-30 Dipolyhydroxystearate, PEG-4 Dilaurate, PEG-40 Sorbitan Peroleate, PEG-60 Almond Glycerides, PEG-7 Olivate, PEG-7 Glyceryl Cocoate, PEG-8 Dioleate, PEG-8 Laurate, PEG-8 Oleate, PEG-80 Sorbitan Laurate, Polysorbate 20, Polysorbate 60, Polysorbate 80, Polysorbate 85, Propylene Glycol Isostearate, Sorbitan Isostearate, Sorbitan Laurate, Sorbitan Monostearate, Sorbitan Oleate, Sorbitan Sesquioleate, Sorbitan Stearate, Sorbitan Trioleate, Stearamide MEA, Steareth-100, Steareth-2, Steareth-20, Steareth-21. The compositions can further include surfactants or combinations of surfactants that create liquid crystalline networks or liposomal networks. Suitable non-limiting examples include OLIVEM 1000 (INCI: Cetearyl Olivate (and) Sorbitan Olivate (available from HallStar Company (Chicago, Ill.)); ARLACEL LC (INCI: Sorbitan Stearate (and) Sorbityl Laurate, commercially available from Croda (Edison, NJ)); CRYSTALCAST MM (INCI: Beta Sitosterol (and) Sucrose Stearate (and) Sucrose Distearate (and) Cetyl Alcohol (and) Stearyl Alcohol, commercially available from MMP Inc. (South Plainfield, NJ)); UNIOX CRISTAL (INCI: Cetearyl Alcohol (and) Polysorbate 60 (and) Cetearyl Glucoside, commercially available from Chemyunion (Sao Paulo, Brazil)). Other suitable emulsifiers include lecithin, hydrogenated lecithin, lysolecithin, phosphatidylcholine, phospholipids, and combinations thereof.
The anti-adherent compositions of the present disclosure may additionally include adjunct ingredients conventionally found in pharmaceutical compositions in an established fashion and at established levels. For example, the anti-adherent compositions may comprise additional compatible pharmaceutically active and compatible materials for combination therapy, such as antioxidants, anti -parasitic agents, antipruritics, antifungals, antiseptic actives, biological actives, astringents, keratolytic actives, local anaesthetics, anti-stinging agents, anti-reddening agents, skin soothing agents, external analgesics, film formers, skin exfoliating agents, sunscreens, and combinations thereof.
Other suitable additives that may be included in the anti-adherent compositions of the present disclosure include compatible colorants, deodorants, emulsifiers, anti-foaming agents (when foam is not desired), lubricants, skin conditioning agents, skin protectants and skin benefit agents (e.g., aloe vera and tocopheryl acetate), solvents, solubilizing agents, suspending agents, wetting agents, pH adjusting ingredients (a suitable pH range of the compositions can be from about 3.5 to about 8), chelators, propellants, dyes and/or pigments, and combinations thereof.
Another component that may be suitable for addition to the anti-adherent compositions is a fragrance. Any compatible fragrance may be used. Typically, the fragrance is present in an amount from about 0% (by weight of the composition) to about 5% (by weight of the composition), and more typically from about 0.01% (by weight of the composition) to about 3% (by weight of the composition). In one desirable embodiment, the fragrance will have a clean, fresh and/or neutral scent to create an appealing delivery vehicle for the end consumer.
Organic sunscreens that may be present in the anti-adherent compositions include ethylhexyl methoxycinnamate, avobenzone, octocrylene, benzophenone-4, phenylbenzimidazole sulfonic acid, homosalate, oxybenzone, benzophenone-3, ethylhexyl salicylate, and mixtures thereof.
As previously noted, in some embodiments, antimicrobial agents can be added to the anti-adherent compositions as optional ingredients. For example, suitable antimicrobials include biocides such as a short-chain alcohol, benzoalkonium chloride (“BAC”), didecyl dimethyl ammonium chloride (“DDAC”), and zeolite (“CWT-A”). Other possible antimicrobial agents include: isothiazolone, alkyl dimethyl ammonium chloride, a triazine, 2-thiocyanomethylthio benzothiazol, methylene bis thiocyanate, acrolein, dodecylguanidine hydrochloride, a chlorophenol, a quaternary ammonium salt, gluteraldehyde, a dithiocarbamate, 2-mercatobenzothiazole, para-chloro-meta-xylenol, silver, chlorohexidine, polyhexamthylene biguanide, a n-halamine, triclosan, a phospholipid, an alpha hydroxyl acid, 2,2-dibromo -3-nitrilopropionamide, 2-bromo-2-nitro-1,3-propanediol, farnesol, iodine, bromine, hydrogen peroxide, chlorine dioxide, a botanical oil, a botanical extract, benzalkonium chloride, chlorine, sodium hypochlorite, or combinations thereof. In some embodiments, the antimicrobial agent can be antibacterial. In some embodiments, the antimicrobial agent can be antiviral. In some embodiments, the antimicrobial agent can be antibacterial and antiviral.
If present, the amount of the antimicrobial agent in the anti-adherent compositions can be in an amount between about 0.01% to about 5% (by total weight of the composition), or in some embodiments between about 0.05% to about 3% (by total weight of the composition). In some embodiments, the antimicrobial agent can be provided to the anti-adherent composition at an effective amount such that a kill of a desired microbe reaches the IC50 (inhibitory concentration 50%) for that desired microbe with that antimicrobial agent in the anti-adherent composition. In some embodiments, the antimicrobial agent can be provided to the anti-adherent composition at less than an effective amount such that a kill of a desired microbe does not reach the IC50 for that desired microbe with that antimicrobial agent in the anti-adherent composition, in which case the anti-adherent composition would still be considered non-antimicrobial for purposes of this disclosure. As previously noted, antimicrobial agents are merely optional ingredients.
The anti-adherent compositions may include various preservatives to increase shelf life. Some suitable preservatives that may be used in the present disclosure include, but are not limited to phenoxyethanol, capryl glycol, glyceryl caprylate, sorbic acid, gallic acid, KATHON CG®, which is a mixture of methylchloroisothiazolinone and methylisothiazolinone, (available from Rohm & Haas Company, Philadelphia, Pa.); DMDM hydantoin (e.g., GLYDANT, available from Lonza, Inc., Fair Lawn, N.J.); EDTA and salts thereof; iodopropynyl butylcarbamate; benzoic esters (parabens), such as methylparaben, propylparaben, butylparaben, ethylparaben, isopropylparaben, isobutylparaben, benzylparaben, sodium methylparaben, and sodium propylparaben; 2-bromo-2-nitropropane-1,3-diol; benzoic acid; and the like. Other suitable preservatives include those sold by Sutton Labs Inc., Chatham, N.J., such as “GERMALL 115” (imidazolidinyl urea), “GERMALL II” (diazolidinyl urea), and “GERMALL PLUS” (diazolidinyl urea and iodopropynyl butylcarbonate).
The amount of the preservative in the anti-adherent compositions is dependent on the relative amounts of other components present within the composition. For example, in some embodiments, the preservative is present in the compositions in an amount between about 0.001% to about 5% (by total weight of the composition), in some embodiments between about 0.01 to about 3% (by total weight of the composition), and in some embodiments, between about 0.05% to about 1.0% (by total weight of the composition).
The anti-adherent compositions of the present disclosure may be prepared by combining ingredients at room temperature and mixing.
In one embodiment, when the anti-adherent composition is to be applied to the skin of an individual, the composition includes the anti-adherent agent, a hydrophilic carrier and a hydrophilic thickener. Suitable hydrophilic carriers can be, for example, water, glycerin, glycerin derivatives, glycols, water-soluble emollients, and combinations thereof. Suitable examples of glycerin derivatives could include, but are not to be limited to, PEG-7 glyceryl cocoate. Suitable glycols could include, but are not to be limited to, propylene glycol, butylene glycol, pentylene glycol, ethoxydiglycol, dipropylene glycol, propanediol, and PEG-8. Suitable examples of water-soluble emollients could include, but are not to be limited to, PEG-6 Caprylic Capric Glycerides, Hydrolyzed Jojoba Esters, and PEG-10 Sunflower Glycerides.
The anti-adherent compositions of the present disclosure may be used in combination with a product. For example, the composition may be incorporated into or onto a substrate, such as a wipe substrate, an absorbent substrate, a fabric or cloth substrate, a tissue or paper towel substrate, or the like. In one embodiment, the anti-adherent composition may be used in combination with a wipe substrate to form a wet wipe or may be a wetting composition for use in combination with a wipe which may be dispersible. In other embodiments, the anti-adherent composition may be incorporated into wipes such as wet wipes, hand wipes, face wipes, cosmetic wipes, cloths and the like. In yet other embodiments, the anti-adherent compositions described herein can be used in combination with numerous personal care products, such as absorbent articles. Absorbent articles of interest are diapers, training pants, adult incontinence products, feminine hygiene products, and the like; bath or facial tissue; and paper towels. Personal protective equipment articles of interest include but are not limited to masks, gowns, gloves, caps, and the like.
In one embodiment, the wet wipe may comprise a nonwoven material that is wetted with an -aqueous solution termed the “wetting composition,” which may include or be composed entirely of the anti adherent compositions disclosed herein. As used herein, the nonwoven material comprises a fibrous material or substrate, where the fibrous material or substrate comprises a sheet that has a structure of individual fibers or filaments randomly arranged in a mat-like fashion. Nonwoven materials may be made from a variety of processes including, but not limited to, airlaid processes, wet-laid processes such as with cellulosic-based tissues or towels, hydroentangling processes, staple fiber carding and bonding, melt blown, and solution spinning.
The fibers forming the fibrous material may be made from a variety of materials including natural fibers, synthetic fibers, and combinations thereof. The choice of fibers may depend upon, for example, the intended end use of the finished substrate and the fiber cost. For instance, suitable fibers may include, but are not limited to, natural fibers such as cotton, linen, jute, hemp, wool, wood pulp, etc. Similarly, suitable fibers may also include: regenerated cellulosic fibers, such as viscose rayon and cuprammonium rayon; modified cellulosic fibers, such as cellulose acetate; or synthetic fibers, such as those derived from polypropylenes, polyethylenes, polyolefins, polyesters, polyamides, polyacrylics, etc. Regenerated cellulose fibers, as briefly discussed above, include rayon in all its varieties as well as other fibers derived from viscose or chemically modified cellulose, including regenerated cellulose and solvent-spun cellulose, such as Lyocell. Among wood pulp fibers, any known papermaking fibers may be used, including softwood and hardwood fibers. Fibers, for example, may be chemically pulped or mechanically pulped, bleached or unbleached, virgin or recycled, high yield or low yield, and the like. Chemically treated natural cellulosic fibers may be used, such as mercerized pulps, chemically stiffened or crosslinked fibers, or sulfonated fibers.
In addition, cellulose produced by microbes and other cellulosic derivatives may be used. As used herein, the term “cellulosic” is meant to include any material having cellulose as a major constituent, and, specifically, comprising at least 50 percent by weight cellulose or a cellulose derivative. Thus, the term includes cotton, typical wood pulps, non-woody cellulosic fibers, cellulose acetate, cellulose triacetate, rayon, thermomechanical wood pulp, chemical wood pulp, debonded chemical wood pulp, milkweed, or bacterial cellulose. Blends of one or more of any of the previously described fibers may also be used, if so desired.
The fibrous material may be formed from a single layer or multiple layers. In the case of multiple layers, the layers are generally positioned in a juxtaposed or surface-to-surface relationship and all or a portion of the layers may be bound to adjacent layers. The fibrous material may also be formed from a plurality of separate fibrous materials wherein each of the separate fibrous materials may be formed from a different type of fiber.
Airlaid nonwoven fabrics are particularly well suited for use as wet wipes. The basis weights for airlaid nonwoven fabrics may range from about 20 to about 200 grams per square meter (gsm) with staple fibers having a denier of about 0.5 to about 10 and a length of about 6 to about 15 millimeters. Wet wipes may generally have a fiber density of about 0.025 g/cc to about 0.2 g/cc. Wet wipes may generally have a basis weight of about 20 gsm to about 150 gsm. More desirably the basis weight may be from about 30 to about 90 gsm. Even more desirably the basis weight may be from about 50 gsm to about 75 gsm.
Processes for producing airlaid non-woven basesheets are described in, for example, published U.S. Pat. App. No. 2006/0008621, herein incorporated by reference to the extent it is consistent herewith.
As a result of the present disclosure, it has been discovered that an anti-adherent composition including an anti-adherent agent can be employed to inhibit the adherence of microbes to a surface. Thus, in one embodiment, an anti-adherent composition can be applied to a surface (e.g., countertop, wall, table, skin, etc.). At least some of the anti-adherent composition can be allowed to remain on the surface such that the anti-adherent agent can inhibit microbes from attaching to the surface, and therefore, reduce the amount of microbes that will adhere to that surface. By reducing the amount of microbes that will adhere to that surface, the anti-adherent composition can be used to reduce the likelihood that individuals come into contact with microbes, and thereby reduce the spreading of the microbes. This benefit could be provided whether the anti-adherent composition was provided in the form of a liquid, gel, or foam; or incorporated into a wash, or is provided on or in a delivery vehicle such as a fibrous substrate (e.g., a wipe).
The disclosure will be more fully understood upon consideration of the following non-limiting examples described in the following section on testing.
From testing conducted and as described further below, it has been discovered that an anti -adherent composition including an anti-adherent agent of the present disclosure can inhibit microbes from attaching or adhering to a surface. The anti-adherent agent can be selected from the group consisting of: Acrylates Copolymer, Trimethylpentanediol/Adipic Acid/Glycerin crosspolymer, Trimethylpentanediol/Adipic Acid Copolymer, Ethylhexyl Stearate, Ethylhexyl Salicylate, Acrylates/C12-22 Alkylmethacrylate Copolymer, Octocrylene, Ethylene Oxide/Propylene Oxide Block Copolymer, and Polyquaternium-101, and any combinations thereof. Anti-adherent compositions including the anti-adherent agents of Acrylates Copolymer and Ethylhexyl Stearate provided an anti-adherent effect against each of the types of microbes Gram Negative bacteria, Gram positive bacteria, DNA viruses, and RNA viruses in the testing conducted and as described further below. Anti-adherent compositions including other anti-adherent agents provided an anti-adherent effect against one or more, but not all, of the types of microbes tested.
Anti-adherent compositions including anti-adherent agents as discussed herein were tested against bacteria using the High Throughput Attachment Test Method. The High Throughput Attachment Test Method is discussed in further detail below. The anti-adherent compositions including the anti -adherent agents were tested against Gram-positive Staphylococcus aureus, and Gram-negative Escherichia coll. The pH of the compositions for this testing between 3 to 10 pH, or about 4 to about 8 pH. The results of the testing is shown below in Table 1.
As shown in the results of Table 1 above and based on the categorization as described by the High Throughput Attachment Test Method (a ratio of bacterial growth on treated pegs to untreated pegs less than 85% being anti-adherent at a statistically significant level), the anti-adherent compositions including the anti-adherent agents of Acrylates Copolymer and Ethylhexyl Stearate provided an anti -adherent effect against both Gram negative bacteria (E. coli) and Gram positive bacteria (S. aureus). The anti-adherent composition including the anti-adherent agent of Trimethylpentanediol/Adipic Acid Copolymer provided an anti-adherent effect against Gram negative bacteria (E. coli), but was neutral -adherent agents of Acrylates/C12-22 Alkylmethacrylate Copolymer, Ethylene Oxide/Propylene Oxide Block Copolymer, Ethylhexyl Salicylate, and Octocrylene provided an anti-adherent effect against Gram positive bacteria (S. aureus), but was neutral against Gram negative bacteria (E. coli).
The anti-adherent compositions that inhibit the attachment of RNA and/or DNA viruses to a surface including compounds serving as anti-adherent agents against RNA and DNA viruses were tested against RNA and DNA viruses via the High Throughput Test to Quantify the Attachment of Phage to a Surface, as discussed in detail below. Tables 2 and 3 below show the variety of compounds that were tested as agents in a composition, as well as the results that related to the percent reduction in viruses and the Logarithmic Reduction compared to growth controls. As will be discussed in further detail below in the discussion regarding the High Throughput Test to Quantify the Attachment of Phage to a Surface, a positive logarithmic reduction in viruses equates to anti-adherent properties (e.g., inhibits attachment), and a negative logarithmic reduction in viruses equates to adherent properties (e.g., increases attachment).
The RNA virus that the compositions were tested against for attachment behaviors was MS2. Bacteriophage are commonly utilized as surrogates of mammalian viruses in both medical and virology applications. MS2 phage is commonly utilized as a viral surrogate because of its size, morphology, environmental stability, non-human infectivity, and the ability for use in high throughput assays. Additionally, MS2 is commonly used as a surrogate to study the spread of human Norovirus (See, Tung -Thompson, et al, PLoS One, 2015, 10(8): e0134277, Dawson D J, et al, J App Micro, 2005, 98: 203-209, Jones, et al, J Hosp Infect, 1991, 17:279-85). The use of MS2 phage in hand sanitizer studies makes it an ideal surrogate to study the interaction of personal care products and viral attachment. It is believed that compositions including the adherent agents noted above would act in a substantially similar behavior to other RNA viruses as they did against MS2.
The DNA virus that the compositions were tested against for attachment behaviors was Phi X 174. Bacteriophage are commonly utilized as surrogates of mammalian viruses in both medical and virology applications. Phi X 174 is commonly utilized as a viral surrogate because of its size, morphology, environmental stability, and non-human infectivity, and the ability for use in high throughput assays. Phi X174 has been previously been used to study barrier efficacy, making it an ideal surrogate to study attachment to a surface (See, Haman and Nelson, Am J Infect Control, 1993, 21:289-96, O'Connell, et al, Clin Microb Infect, 2004, 10:322-6, ASTM F1671 /F1671M-13, Standard Test Method for Resistance of Materials Used in Protective Clothing to Penetration by Blood-Borne Pathogens Using Phi X174 Bacteriophage Penetration as a Test System). Thus, it is well accepted by those of ordinary skill in the art that Phi X 174 serves as a surrogate for other DNA viruses, and the compositions including the adherent agents noted above would act in a substantially similar behavior to other DNA viruses as they did against Phi X 174.
As can be seen from Table 2, several of the compounds exhibited positive logarithmic reductions in testing against RNA viruses at statistically significant levels, and thus, can help to inhibit adherence of an RNA virus to a surface. Additionally, as can be seen from Table 3 below, several of the compounds exhibited positive logarithmic reductions in testing against DNA viruses at statistically significant levels, and thus, can help to inhibit adherence of a DNA virus to a surface. The anti-adherent compositions including the anti-adherent agents of Acrylates Copolymer, Trimethylpentanediol/Adipic Acid/Glycerin Crosspolymer, Trimethylpentanediol/Adipic Acid Copolymer, Ethylhexyl Stearate, and Ethylhexyl Salicylate provided an anti-adherent effect against both RNA viruses and DNA viruses. The anti-adherent compositions including the anti-adherent agent of Polyquaternium-101 provided an anti-adherent effect against RNA viruses, but was neutral against DNA viruses. The anti-adherent compositions including the anti-adherent agents of Acrylates/C12-22 Alkylmethacrylate Copolymer, Ethylene Oxide/Propylene Oxide Block Copolymer, and Octocrylene provided an anti-adherent effect against DNA viruses, but were neutral against RNA viruses.
This test method specifies the operational parameters required to grow and or prevent the formation of bacterial attachment using a high throughput screening assay. The assay device consists of a plastic lid with ninety-six (96) pegs and a corresponding receiver plate with ninety-six (96) individual wells that have a maximum 200 μL working volume. Biofilm is established on the pegs under static batch conditions (i.e., no flow of nutrients into or out of an individual well).
1.2 Definitions of Terms Specific to This Standard:
1.2.2 peg, n—biofilm sample surface (base: 5.0 mm, height: 13.1 mm).
1.2.3 peg lid, n—an 86×128 mm plastic surface consisting of ninety-six (96) identical pegs.
1.2.4 plate, n—an 86×128 mm standard plate consisting of ninety-six (96) identical wells.
1.2.5 well, n—small reservoir with a 50 to 200 μL working volume capacity.
2.2 ATCC: American Type Culture Collection
2.3 CFU: colony forming unit
2.4 rpm: revolutions per minute
2.5 SC: sterility control
2.6 TSA: tryptic soy agar
2.7 TSB: tryptic soy broth
2.8 GC: growth control
3.2 Inoculating loop—nichrome wire or disposable plastic.
3.3 Petri dish—large labelled (100×150×15 mm, plastic, sterile) for plating.
3.4 Microcentrifuge tubes—sterile, any with a 1.5 mL volume capacity.
3.5 96-well microtiter plate—sterile, 86×128 mm standard plate consisting of ninety -six (96) identical flat bottom wells with a 200 μL working volume
3.6 Vortex—any vortex that will ensure proper agitation and mixing of microfuge tubes.
3.7 Pipette—continuously adjustable pipette with volume capability of 1 mL.
3.8 Micropipette—continuously adjustable pipette with working volume of 10 μL-200 μL.
3.9 Sterile pipette tips—200 μL and 1000 μL volumes.
3.10 Sterile reagent reservoir—50 mL polystyrene.
3.11 Sterilizer—any steam sterilizer capable of producing the conditions of sterilization.
3.12 Colony counter—any one of several types may be used. A hand tally for the recording of the bacterial count is recommended if manual counting is done.
3.13 Environmental incubator—capable of maintaining a temperature of 35±2° C. and relative humidity between 35 and 85%.
3.14 Reactor components—the MBEC Assay device available from Innovotech, Edmonton, AB, Canada.
3.15 Sterile conical tubes—50 mL, used to prepare initial inoculum.
3.16 Appropriate glassware—as required to make media and agar plates.
3.17 Erlenmeyer flask—used for growing broth inoculum.
3.18 Positive Displacement pipettes capable of pipetting 200 μL.
3.19 Sterile pipette tips appropriate for Positive Displacement pipettes.
4.2 Purity of water—all references to water as diluent or reagent shall mean distilled water or water of equal purity.
4.3 Culture media:
4.4 Bacterial growth broth—Tryptic soy broth (TSB) prepared according to manufacturer's directions.
4.5 Bacterial plating medium—Tryptic soy agar (TSA) prepared according to manufacturer's directions.
4.6 Phosphate Buffered Saline (PBS)—
4.7 Rinse Solution: Sterile PBS and TWEEN 80 (Sigma-Aldrich, St. Louis, Mo. 1% w/v.
5.1 E. coli ATCC 11229 and S. aureus ATCC 6538
6. TEST METHOD overview: The experimental process for the High-Throughput Anti-Adherence Test Method. This standard protocol may be broken into a series of small steps, each of which is detailed in the sections below.
6.1 Culture Preparation
6.1.1 E. coli ATCC 11229 and S. aureus ATCC 6538 are the organisms used in this test.
6.1.2 Using a cryogenic stock (at −70° C.), streak out a subculture of the above listed microorganisms on organism's specific agar (TSA).
6.1.3 Incubate at 35±2° C. for the period of time of 22±2 hours.
6.1.4 Aseptically remove isolated colony from streak plate and inoculate 20 mL of sterile TSB.
6.1.5 Incubate flask at 35±2° C. and 175±10 rpm for 16 to 18 hours (E. coli and S. aureus). Viable bacterial density should be 109 CFU/mL and should be checked by serial dilution and plating.
6.1.6 Pipette 10 mL from the incubation flask of E. coli and S. aureus into a 50 mL conical tube and spin down at 5 minutes at 4,000×g. Then remove supernatant and Resuspend in 10 mL sterile PBS. Approximate cell density should be 107-109 CFU/mL. Vortex the sample for approximately 30 seconds to achieve a homogeneous distribution of cells.
6.1.7 Perform 10-fold serial dilutions of the inoculum in triplicate.
6.1.8 Plate appropriate dilutions on appropriately labelled TSA plates. Incubate the plates at 35±2° C. for 22±2 hours depending on the isolates growth rate and enumerate.
6.2 Preparation of the Challenge plates:
6.2.1 Preparation of compounds and coating compounds onto MBEC plate lid
6.2.1.1.1 Using a positive displacement pipette aseptically add 200 μL of compounds and control to be tested to a sterile 96-well microplate according to the plate layout of Table 4 below.
E. coli
E. coli
E. coli
E. coli
S. aureus
S. aureus
S. aureus
S. aureus
6.2.1.1.2 Add 200 μL of each code to the appropriate well for sterility controls.
6.2.1.1.3 Place the MBEC plate lid, peg side down into the 96-well microplate containing the test compound solutions.
6.2.1.1.4 Allow the plate to sit at room temperature (25±3° C.) for 2 hours.
6.2.1.1.5 Remove the MBEC plate lid and allow the lid to dry at room temperature (25±3° C.) overnight in a laminar flow hood.
7.1.1 Add 100 μL of diluted bacteria to the appropriate wells in a sterile 96-well microplate as indicated in the plate layout in Table 4.
7.1.2 Add 200 μL of sterile PBS to the sterility controls.
7.1.3 The MBEC containing dried compounds is then inserted into the bacterial inoculated 96 well flat bottom microplate from section 9.3.1
7.1.4 Incubate stationary at room temperature (25±3° C.) for 15 minutes.
7.1.5 Remove the MBEC lid and place into a 96-well microplate containing 200 μL PBS +1% w/v TWEEN 80. Incubate stationary at room temperature (25±3 ° C.) for 15 seconds.
7.1.6 Repeat step 7.1.5 for two additional washes for a total of 3 washes.
7.2.1 Transfer the washed MBEC plate lid to a 96-well plate containing 200 μL ALAMARBLUE reagent (prepared according to manufacturer's directions, Life Technologies, Carlsbad, Calif.) in each well to be tested.
7.2.2 The final plate is transferred to a SPECTRAMAX GEMINI EM microplate reader (Molecular Devices, Inc. Sunnyvale, Calif. USA) for a 20 hour kinetic, bottom read with an excitation of 560 nm and emission of 590 nm. The rate of fluorescence development (relative fluorescence units (RFU)/minute) is determined for each well.
7.2.3 From these data, the ratio of bacterial growth on the test codes pegs to the bacterial growth on the nontreated growth control pegs was calculated.
If the ratio of bacterial growth on the test code pegs to the bacterial growth on the nontreated growth control pegs is less than 85%, the agent is deemed to be anti-adherent;
If the ratio of bacterial growth on the test code pegs to the bacterial growth on the nontreated growth control pegs is between 85% -115%, the agent is deemed to be neutral (neither adherent nor anti-adherent); and
If the ratio of bacterial growth on the test code pegs to the bacterial growth on the nontreated growth control pegs is greater than 115%, the agent is deemed to be adherent.
Growth and purification of phage is outlined in the following steps.
1.1 Subculture: (these steps ensured that the organism are less than 5 generations removed from the original clinical isolate):
1.2 Prepare Top Agar:
1.3 Preparation of bacterial host:
1.4 Propagation of the Phage:
1.5 Phage (MS2 and PhiX 174) Enumeration:
1.6 Preparation of the Challenge plates:
1.7 Phage attachment to MBEC Lids:
1.8 Phage Recovery:
1.9 LOG10 Reduction:
1.9.7 Cell Enumeration:
LOG10(PFU/peg)=LOG10[(X/B) (D)] where:
X=mean PFU,
B=volume plated (0.02 mL)
and D=dilution.
Calculate the overall attached bacteria accumulation by calculating the mean of the log densities calculated.
Calculate the LOG10 reduction for each dilution as follows: LOG10 Reduction=Mean LOG10 Growth Control−Mean LOG10 Test.
Calculate the Percent Reduction by calculating (Log10(PFU/Peg) of the growth control pegs−Log10(PFU/Peg) of the treated pegs)/Log10(PFU/Peg) of the growth control pegs)×100
1.10 Accept or Reject Criteria
Embodiment 1: A method of inhibiting microbes from attaching to a surface, the method comprising: providing an anti-adherent composition, the anti-adherent composition comprising: an anti-adherent agent configured to inhibit microbes from attaching to the surface, the anti-adherent agent selected from the group consisting of: Acrylates Copolymer, Trimethylpentanediol/Adipic Acid/Glycerin crosspolymer, Trimethylpentanediol/Adipic Acid Copolymer, Ethylhexyl Stearate, Ethylhexyl Salicylate, Acrylates/C12-22 Alkylmethacrylate Copolymer, Octocrylene, Ethylene Oxide/Propylene Oxide Block Copolymer, and Polyquaternium-101, and any combinations thereof; applying the composition to the surface; and allowing at least some of the composition to remain on the surface such that the anti-adherent agent inhibits microbes from attaching to the surface.
Embodiment 2: The method of embodiment 1, wherein the composition further comprises a humectant.
Embodiment 3: The method of embodiment 2, wherein the humectant is selected from the group consisting of: glycerin, a glycerin derivative, and combinations thereof.
Embodiment 4: The method of any one of the preceding embodiments, wherein the composition further comprises an ingredient selected from the group consisting of an emollient, a surfactant, an antimicrobial agent, and any combination thereof.
Embodiment 5: The method of any one of the preceding embodiments, wherein the composition is non -antimicrobial.
Embodiment 6: The method of any one of the preceding embodiments, wherein the microbes comprise Gram negative bacteria, and the anti-adherent agent is selected from the group consisting of: Acrylates Copolymer, Trimethylpentanediol/Adipic Acid Copolymer, and Ethylhexyl Stearate.
Embodiment 7: The method of any one of embodiments 1-5, wherein the microbes comprise Gram positive bacteria, and the anti-adherent agent is selected from the group consisting of: Acrylates Copolymer, Ethylhexyl Stearate, Ethylhexyl Salicylate, Acrylates/C12-22 Alkylmethacrylate Copolymer, Octocrylene, and Ethylene Oxide/Propylene Oxide Block Copolymer.
Embodiment 8: The method of any one of embodiments 1-5, wherein the microbes comprise a DNA virus, and the anti-adherent agent is selected from the group consisting of: Acrylates Copolymer, Trimethylpentanediol/Adipic Acid/Glycerin crosspolymer, Trimethylpentanediol/Adipic Acid Copolymer, Ethylhexyl Stearate, Ethylhexyl Salicylate, Acrylates/C12-22 Alkylmethacrylate Copolymer, Octocrylene, and Ethylene Oxide/Propylene Oxide Block Copolymer.
Embodiment 9: The method of any one of embodiments 1-5, wherein the microbes comprise an RNA virus, and the anti-adherent agent is selected from the group consisting of: Acrylates Copolymer, Trimethylpentanediol/Adipic Acid/Glycerin crosspolymer, Trimethylpentanediol/Adipic Acid Copolymer, Ethylhexyl Stearate, Ethylhexyl Salicylate, and Polyquaternium-101.
Embodiment 10: An anti-adherent composition for preventing microbes from attaching to a surface, the anti-adherent composition comprising: an anti-adherent agent selected from the group consisting of: Trimethylpentanediol/Adipic Acid/Glycerin crosspolymer, Trimethylpentanediol/Adipic Acid Copolymer, Ethylhexyl Stearate, and Acrylates/C12-22 Alkylmethacrylate Copolymer, and any combinations thereof; and a humectant.
Embodiment 11: The anti-adherent composition of embodiment 10, wherein the humectant is selected from the group consisting of: glycerin, glycerin derivatives, hyaluronic acid derivatives, betaine derivatives amino acids, amino acid derivatives, glycosaminoglycans, glycols, polyols, sugars, sugar alcohols, hydrogenated starch hydrolysates, hydroxy acids, hydroxy acid derivatives, salts of PCA, and any combination thereof.
Embodiment 12: The anti-adherent composition of embodiment 10, wherein the humectant is selected from the group consisting of: honey, sorbitol, hyaluronic acid, sodium hyaluronate, betaine, lactic acid, citric acid, sodium citrate, glycolic acid, sodium glycolate ,sodium lactate, urea, propylene glycol, butylene glycol, pentylene glycol, ethoxydiglycol, methyl gluceth-10, methyl gluceth-20, PEG-2, PEG-3, PEG-4, PEG-5, PEG-6, PEG-7, PEG-8, PEG-9, PEG-10, xylitol, maltitol, and any combination thereof.
Embodiment 13: The anti-adherent composition of embodiment 10, wherein the humectant is selected from the group consisting of: glycerin, a glycerin derivative, and combinations thereof.
Embodiment 14: The anti-adherent composition of any one of embodiments 10-13, wherein the composition further comprises an ingredient selected from the group consisting of an emollient, a surfactant, an antimicrobial agent, and any combination thereof.
Embodiment 15: The anti-adherent composition of any one of embodiments 10-14, wherein the composition is non-antimicrobial.
Embodiment 16: The anti-adherent composition of any one of embodiments 10-15, wherein the anti-adherent agent is present in the amount of about 0.01% to about 20.0% by weight of the composition, and wherein the humectant is present in the amount of about 0.01% to about 20.0% by weight of the composition.
Embodiment 17: An anti-adherent composition for preventing microbes from attaching to a surface, the anti-adherent composition comprising: an anti-adherent agent selected from the group consisting of: Acrylates Copolymer, Trimethylpentanediol/Adipic Acid/Glycerin Crosspolymer, Trimethylpentanediol/Adipic Acid Copolymer, Ethylhexyl Stearate, Ethylhexyl Salicylate, Acrylates/C12-22 Alkylmethacrylate Copolymer, and Polyquaternium-101, and any combinations thereof; and a humectant; wherein the anti-adherent composition is non-antimicrobial.
Embodiment 18: The anti-adherent composition of embodiment 17, wherein the humectant is selected from the group consisting of: glycerin, a glycerin derivative, and combinations thereof.
Embodiment 19: The anti-adherent agent composition of embodiment 17 or 18, wherein the anti-adherent agent is present in the amount of about 0.01% to about 20.0% by weight of the composition, and wherein the humectant is present in the amount of about 0.01% to about 20.0% by weight of the composition.
Embodiment 20: A wipe for inhibiting the attachment of microbes to a surface, the wipe comprising: a nonwoven substrate; and the anti-adherent composition according to any one of embodiments 10-19.
When introducing elements of the present disclosure, the articles “a”, “an”, “the” and “said” are intended to mean that there are one or more of the elements. The terms “comprising”, “including” and “having” are intended to be inclusive and mean that there may be additional elements other than the listed elements. Many modifications and variations of the present disclosure can be made without departing from the spirit and scope thereof. Therefore, the exemplary embodiments described above should not be used to limit the scope of the disclosure.
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/US2016/034252 | 5/26/2016 | WO | 00 |
