Claims
- 1. A method of detecting binding of members of a library of compounds to a cellular or protein target, wherein said members of said library are characterized by comprising a galactosyl epitope, said method comprising:
- contacting said members of said library with said target under conditions for binding of said members of said library to said target, where the interaction of said target with said members of said library is individually distinguishable;
- washing away non-specifically bound members of said library from said target at a predetermined stringency;
- contacting said target with a composition comprising primate anti-galactosyl antibodies and reagent for providing a detectable signal in relation to the presence of said primate anti-galactosyl antibodies; and
- detecting said detectable signal, whereby the presence of said detectable signal in relation to a specific member of said library indicates the specific binding of said specific member to said target.
- 2. A method according to claim 1, wherein said target is cells.
- 3. A method according to claim 1, wherein said members are linked to a solid substrate prior to contacting with said cells.
- 4. A method according to claim 1, wherein said members are oligomers.
- 5. A method according to claim 4, wherein said oligomers are oligopeptides.
- 6. A method according to claim 4, wherein said oligomers are oligonucleotides.
Parent Case Info
This application is a divisional of U.S. Ser. No. 08/740,166, filed Oct. 22, 1996, now U.S. Pat. No. 5,714,332, issued Feb. 3, 1998, which claims the benefit of provisional application Ser. No. 60/006,044, filed Oct. 24, 1995, now abandoned.
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Jul 1993 |
WOX |
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Non-Patent Literature Citations (4)
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Galili, U., et al., "Evolutionary Relationship Between the Natural Anti-Gal Antibody and the Gal.alpha.1.fwdarw.3Gal Epitope in Primates," PNAS, USA, 84:1369-1373 (1987). |
Galili, U., et al., "One Percent of Human Circulationg B Lymphocytes are Capable of Producing the Natural Anti-Gal Antibody," Blood, 82(8):2485-2493 (1993). |
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Divisions (1)
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Number |
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Parent |
740166 |
Oct 1996 |
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