Claims
- 1. A method of treating or preventing a pathogen infection in a cell, comprising administering to said cell chimeric molecules having at least one pathogen-detection domain and at least one effector domain, said pathogen-detection domain being one not naturally bound to said effector domain, whereby in the presence of a pathogen in the cell, said chimeric molecules bind to the pathogen and activate said effector domain, thereby treating or preventing the pathogen infection in said cell.
- 2. A method of treating or preventing a pathogen infection in a cell, comprising administering to said cell chimeric molecules having at least one pathogen-induced product-detection domain and at least one effector domain, said pathogen-induced product-detection domain being one not naturally bound to said effector domain, whereby in the presence of a pathogen-induced product in said cell, the chimeric molecules bind to the pathogen-induced product and activate said effector domain, thereby treating or preventing the pathogen infection in said cell.
- 3. A method of treating or preventing the spread of a pathogen infection in an organism, comprising administering to said organism chimeric molecules having at least one pathogen-detection domain and at least one effector domain, said pathogen-detection domain being one not naturally bound to said effector domain, whereby in the presence of a pathogen in a cell of said organism, said chimeric molecules bind to the pathogen and activate said effector domain, thereby treating or preventing the spread of said pathogen infection in said organism.
- 4. A method of treating or preventing the spread of a pathogen infection in an organism, comprising administering to said organism chimeric molecules having at least one pathogen-induced product-detection domain and at least one effector domain, said pathogen-induced product-detection domain being one not naturally bound to said effector domain, whereby in the presence of a pathogen-induced product in a cell of said organism, said chimeric molecules bind to the pathogen-induced product and activate said effector domain, thereby treating or preventing the spread of said pathogen infection in said organism.
- 5. A method of treating or preventing a pathogen infection in a cell, comprising administering to said cell an agent, wherein said agent comprises at least one pathogen-interacting molecular structure and at least one effector-mediating molecular structure, said agent being one that is non-naturally-occurring in said cell, whereby in the presence of a pathogen in said cell, the agent binds to the pathogen and activates the effector-mediating molecular structure, thereby treating or preventing the pathogen infection in said cell.
- 6. A method of treating or preventing a pathogen infection in a cell, comprising administering to said cell an agent, wherein said agent comprises at least one pathogen-induced product-interacting molecular structure and at least one effector-mediating molecular structure, said agent being one that is non-naturally-occurring in a cell, whereby in the presence of a pathogen-induced product in said cell, the agent binds to the pathogen-induced product and activates the effector-mediating molecular structure, thereby treating or preventing the pathogen infection in said cell.
- 7. A method of treating or preventing the spread of a pathogen infection in an organism, comprising administering to said organism an agent, wherein said agent comprises at least one pathogen-interacting molecular structure and at least one effector-mediating molecular structure, said agent being one that is non-naturally-occurring in a cell, whereby in the presence of a pathogen in a cell of said organism, the agent binds to the pathogen and activates the effector-mediating molecular structure, thereby treating or preventing the spread of said pathogen infection in said organism.
- 8. A method of treating or preventing the spread of a pathogen infection in an organism, comprising administering to said organism an agent, wherein said agent comprises at least one pathogen-induced product-interacting molecular structure and at least one effector-mediating molecular structure, said agent being one that is non-naturally-occurring in a cell, whereby in the presence of a pathogen-induced product in a cell of said organism, the agent binds to the pathogen-induced product and activates the effector-mediating molecular structure, thereby treating or preventing the spread of said pathogen infection in said organism.
- 9. A method of treating or preventing a pathogen infection in a cell, comprising administering to said cell individual components of a chimeric molecule, wherein said components of said chimeric molecule assemble together to form the chimeric molecule, said assembled chimeric molecule having at least one pathogen-detection domain and at least one effector domain, said pathogen-detection domain being one not naturally assembled with said effector domain, whereby in the presence of a pathogen in said cell, the chimeric molecule binds to the pathogen and activates said effector domain, thereby treating or preventing a pathogen infection in said cell.
- 10. A method of treating or preventing a pathogen infection in a cell, comprising administering to said cell individual components of a chimeric molecule, wherein said components of said chimeric molecule assemble together to form the chimeric molecule, said assembled chimeric molecule having at least one pathogen-induced product-detection domain and at least one effector domain, said pathogen-induced product-detection domain being one not naturally assembled with said effector domain, whereby in the presence of a pathogen-induced product in said cell, the chimeric molecule binds to the pathogen-induced product and activates said effector domain, thereby treating or preventing a pathogen infection in said cell.
- 11. A method of treating or preventing the spread of a pathogen infection in an organism, comprising administering to said organism individual components of a chimeric molecule, wherein said components of said chimeric molecule assemble together to form the chimeric molecule, said assembled chimeric molecule having at least one pathogen-detection domain and at least one effector domain, said pathogen-detection domain being one not naturally assembled with said effector domain, whereby in the presence of a pathogen in a cell of said organism, said chimeric molecule binds to the pathogen and activates said effector domain, thereby treating or preventing the spread of a pathogen infection in said organism.
- 12. A method of treating or preventing the spread of a pathogen infection in an organism, comprising administering to said organism individual components of a chimeric molecule, wherein said components of said chimeric molecule assemble together to form the chimeric molecule, said assembled chimeric molecule having at least one pathogen-induced product-detection domain and at least one effector domain, said pathogen-induced product-detection domain being one not naturally assembled with said effector domain, whereby in the presence of a pathogen-induced product in a cell of said organism, said chimeric molecule binds to the pathogen-induced product and activates said effector domain, thereby treating or preventing the spread of a pathogen infection in said organism.
- 13. A chimeric molecule having at least one pathogen-detection domain and at least one effector domain, said chimeric molecule being one that is non-naturally-occurring in a cell.
- 14. A chimeric molecule having at least one pathogen-induced product-detection domain and at least one effector domain, said chimeric molecule being one that is non-naturally-occurring in a cell.
- 15. An agent having at least one pathogen-interacting molecular structure and at least one effector-mediating molecular structure, said agent being one that is non-naturally-occurring in a cell.
- 16. An agent having at least one pathogen-induced product-interacting molecular structure and at least one effector-mediating molecular structure, said agent being one that is non-naturally-occurring in a cell.
- 17. An assay for the detection of a pathogen infection in a cell, comprising the steps of:
a) culturing said cell in a suitable cell culture medium; b) administering to said cell a chimeric molecule having at least one pathogen-detection domain and at least one effector domain, said chimeric molecule being one that is non-naturally-occurring in a cell, whereby in the presence of a pathogen in said cell, said chimeric molecule binds to said pathogen and activates said effector domain; and c) determining the presence or absence of effector domain activation; whereby activation of said effector domain indicates the presence of a pathogen infection in said cell.
- 18. An assay for the detection of a pathogen infection in an organism, comprising the steps of:
a) obtaining a cell from said organism; b) culturing said cell in a suitable cell culture medium; c) administering to said cell a chimeric molecule having at least one pathogen-detection domain and at least one effector domain, said chimeric molecule being one that is non-naturally-occurring in a cell, whereby in the presence of a pathogen in said cell, said chimeric molecule binds to said pathogen and activates said effector domain; and d) determining the presence or absence of effector domain activation; whereby activation of said effector domain indicates the presence of a pathogen infection in said cell in said organism.
- 19. An assay for the detection of a pathogen infection in an organism, comprising the steps of:
a) obtaining a sample from said organism; b) adding said sample to an uninfected cell; c) culturing said cell in a suitable cell culture medium; d) administering to said cell a chimeric molecule having at least one pathogen-detection domain and at least one effector domain, said chimeric molecule being one that is non-naturally-occurring in a cell, whereby in the presence of a pathogen in said cell, said chimeric molecule binds to said pathogen and activates said effector domain; and e) determining the presence or absence of effector domain activation; whereby activation of said effector domain indicates the presence of a pathogen infection in the sample obtained from said organism.
- 20. An assay for the detection of a pathogen infection in a cell, comprising:
a) culturing said cell in a suitable cell culture medium; b) administering to said cell an agent having at least one pathogen-interacting molecular structure and at least one effector-mediating molecular structure, said agent being one that is non-naturally-occurring in said cell, whereby in the presence of a pathogen in said cell, said agent binds to said pathogen and activates said effector-mediating molecular structure; and c) determining the presence or absence of effector-mediating molecular structure activation; whereby activation of said effector-mediating molecular structure indicates the presence of a pathogen infection in said cell.
- 21. An assay for the detection of a pathogen infection in an organism, comprising the steps of:
a) obtaining a cell from said organism; b) culturing said cell in a suitable cell culture medium; c) administering to said cell an agent having at least one pathogen-interacting molecular structure and at least one effector-mediating molecular structure, said agent being one that is non-naturally-occurring in said cell, whereby in the presence of a pathogen in said cell, said agent binds to said pathogen and activates said effector-mediating molecular structure; and d) determining the presence or absence of effector-mediating molecular structure activation; whereby activation of said effector-mediating molecular structure indicates the presence of a pathogen infection in said organism.
- 22. An assay for the detection of a pathogen infection in a cell, comprising:
a) culturing said cell in a suitable cell culture medium; b) administering to said cell a chimeric molecule having at least one pathogen-induced product-detection domain and at least one effector domain, said chimeric molecule being one that is non-naturally-occurring in a cell, whereby in the presence of a pathogen-induced product in said cell, said chimeric molecule binds to said pathogen-induced product and activates said effector domain; and c) determining the presence or absence of effector domain activation; whereby activation of said effector domain indicates the presence of a pathogen infection in said cell.
- 23. An assay for the detection of a pathogen infection in an organism, comprising:
a) obtaining a cell from said organism; b) culturing said cell in a suitable cell culture medium; c) administering to said cell a chimeric molecule having at least one pathogen-induced product-detection domain and at least one effector domain, said chimeric molecule being one that is non-naturally-occurring in a cell, whereby in the presence of a pathogen-induced product in said cell, said chimeric molecule binds to said pathogen-induced product and activates said effector domain; and d) determining the presence or absence of effector domain activation; whereby activation of said effector domain indicates the presence of a pathogen infection in said cell in said organism.
- 24. An assay for the detection of a pathogen infection in an organism, comprising:
a) obtaining a sample from said organism; b) adding said sample to an uninfected cell; c) culturing said cell in a suitable cell culture medium; d) administering to said cell a chimeric molecule having at least one pathogen-induced product-detection domain and at least one effector domain, said chimeric molecule being one that is non-naturally-occurring in a cell, whereby in the presence of a pathogen-induced product in said cell, said chimeric molecule binds to said pathogen-induced product and activates said effector domain; and e) determining the presence or absence of effector domain activation; whereby activation of said effector domain indicates the presence of a pathogen infection in the sample obtained from said organism.
- 25. An assay for the detection of a pathogen infection in a cell, comprising:
a) culturing said cell in a suitable cell culture medium; b) administering to said cell an agent having at least one pathogen-induced product-interacting molecular structure and at least one effector-mediating molecular structure, said agent being one that is non-naturally-occurring in said cell, whereby in the presence of a pathogen-induced product in said cell, said agent binds to said pathogen-induced product and activates said effector-mediating molecular structure; and c) determining the presence or absence of effector-mediating molecular structure activation; whereby activation of said effector-mediating molecular structure indicates the presence of a pathogen infection in said cell.
- 26. An assay for the detection of a pathogen infection in an organism, comprising:
a) obtaining a cell from said organism; b) culturing said cell in a suitable cell culture medium; c) administering to said cell an agent having at least one pathogen-induced product-interacting molecular structure and at least one effector-mediating molecular structure, said agent being one that is non-naturally-occurring in said cell, whereby in the presence of a pathogen-induced product in said cell, said agent binds to said pathogen-induced product and activates said effector-mediating molecular structure; and d) determining the presence or absence of effector-mediating molecular structure activation; whereby activation of said effector-mediating molecular structure indicates the presence of a pathogen infection in said organism.
RELATED APPLICATIONS
[0001] This application claims the benefit of U.S. Provisional Application No. 60/355,359, filed Feb. 7, 2002, U.S. Provisional Application No. 60/355,022, filed Feb. 7, 2002, and U.S. Provisional Application No. 60/432,386, filed Dec. 10, 2002.
[0002] The entire teachings of the above applications are incorporated herein by reference.
GOVERNMENT SUPPORT
[0003] The invention was supported, in whole or in part, by contract number F19628-00-C-0002 from the United States Air Force. The Government has certain rights in the invention.
Provisional Applications (3)
|
Number |
Date |
Country |
|
60355359 |
Feb 2002 |
US |
|
60355022 |
Feb 2002 |
US |
|
60432386 |
Dec 2002 |
US |