Antibacterial solid surface materials containing chitosan-metal complexes

Abstract

A solid surface material with an antimicrobial agent in a thermoset and/or thermoplastic resin matrix where the antimicrobial agent comprises a chitosan-metal complex.

Description

FIELD OF INVENTION

[0001] This invention is directed to solid surface materials having antimicrobial properties.

BACKGROUND OF THE INVENTION

[0002] Artificial or synthetic marble is a general designation for various types of materials used as building products, such as bathroom vanity tops, sinks, shower stalls and kitchen counter tops, and other decorative surfaces. It is also a suitable material for use in furniture, lining materials, and in stationary small articles. The artificial marble is easily kept clean and neat. Therefore, it has increasingly been used in hospitals, nursing homes, as well as in commercial and residential food preparation facilities.

[0003] Artificial marbles encompass cultured marble, onyx and solid surface materials typically comprising some kind of resin matrix and either with or without a filler present in the resin matrix. Typically, cultured marble is made of a gel coating of unfilled unsaturated polyester on a substrate of a filled unsaturated polyester. The filler may be calcium carbonate or a similar material. Onyx typically consists of a gel coat of unfilled unsaturated polyester on a substrate of filled unsaturated polyester. The filler in this case is typically alumina trihydrate (ATH). Solid surface materials are typically filled resin materials and, unlike cultured marble or onyx, do not have a gel coat. Corian® material available from E. I. du Pont de Nemours and Company (DuPont), Wilmington, Del., is a solid surface material comprising an acrylic matrix filled with ATH. Another solid surface DuPont material, known by the brand name Zodiaq®, is alternatively described as an engineered stone or artificial granite. Such materials are made from an unsaturated polyester matrix filled with quartz or other similar fillers.

[0004] As evidenced by numerous materials in the market, there is clearly a demand for materials and/or processes that either minimize or kill harmful microorganisms encountered in the environment. Such materials are useful in areas for food preparation, processing, service or handling. Such materials will also be useful in areas for personal hygiene, such as bathroom facilities. Similarly, there is a use for such antimicrobial materials in hospitals and nursing homes where people with lowered resistance are especially vulnerable to pathogenic microorganisms.

[0005] Solid surface materials made of either an acrylic resin, an unsaturated polyester resin, an epoxy resin, or other such resins and incorporating certain antimicrobial agents throughout the resin are described in WO 97/49761 (E. I. du Pont de Nemours and Company). However, such antimicrobial agents can be expensive, resulting in a high installation cost for the resulting solid surface material.

[0006] Chitosan and chitosan-metal compounds are known to provide antimicrobial activity as bacteriocides and fungicides (see, e.g., T. L. Vigo, “Antimicrobial Polymers and Fibers: Retrospective and Prospective,” in Bioactive Fibers and Polymers, J. V. Edwards and T. L. Vigo, eds., ACS Symposium Series 792, pp.175-200, American Chemical Society, 2001). Chitosan is also known to impart antiviral activity, though the mechanism is not yet well understood (see, e.g., Chirkov, S. N., Applied Biochemistry and Microbiology (Translation of Prikladnaya Biokhimiya i Mikrobiologiya) (2002), 38(1), 1-8).

[0007] Chitosan is the commonly used name for poly-[1-4]-β-D-glucosamine. Chitosan is chemically derived from chitin (a poly-[1-4]-β-N-acetyl-D-glucosamine) which, in turn, is derived from the cell walls of fungi, the shells of insects, and, especially, crustaceans. Thus, it is inexpensively derived from widely available materials. It is available as an article of commerce from, for example, Primex (Iceland); Biopolymer Engineering, Inc. (St. Paul, Minn.); Biopolymer Technologies, Inc. (Westborough, Mass.); and CarboMer, Inc. (Westborough, Mass.). Chitosan can also be treated with metal-salt solutions so that the metal ion forms a complex with the chitosan. For example, U.S. Pat. Nos. 5,541,233 and 5,643,971 disclose a process for preparing durable antimicrobial agents by treating a chitosan suspension with metal salts of zinc and copper followed by chelation of a potentiator such as an imidazole. Application WO 99/37584 discloses the preparation of chitosan-zinc sulfate, copper sulfate and silver nitrate complexes for treating water to reduce levels of pathogens.

[0008] In commonly assigned U.S. patent application Ser. No. 60/290,297 (filed May 11, 2001), chitosan (in the form of an acidic solution applied to polyester articles) is shown to impart antimicrobial activity. The chitosan-treated article may be treated subsequently with a solution of zinc sulfate, cupric sulfate, or silver nitrate to enhance antimicrobial activity.

[0009] Cultured marbles have been developed incorporating an antimicrobial agent in the gel coat only (i.e., not throughout the matrix of the substrate). Such materials have been disclosed in Japanese Patent Application Publication Kokai: 7-266522. These materials have a relatively thin gel coat, typically on the order of 15 mils. As such, when the gel coat is depleted of antimicrobial agent or the gel coat wears away or is otherwise removed, the antimicrobial effect of the gel coat is significantly decreased or lost entirely.

[0010] The problem that remains to be solved is to provide solid surface materials comprising either an acrylic resin, an unsaturated polyester resin, an epoxy or other similar resin and an effective antimicrobial agent dispersed throughout the resin.

SUMMARY OF THE INVENTION

[0011] This invention is directed to a solid surface material comprising a matrix of at least one resin, and an antimicrobial agent dispersed in the matrix. The antimicrobial agent is a chitosan-metal complex, which is prepared under homogeneous conditions and isolated as a product. The resin can be thermoset, thermoplastic, or combinations thereof. Optionally, at least one filler can be dispersed in the matrix.

[0012] In a preferred embodiment, the resin is made from a syrup comprising an acrylic group polymer dissolved in a material selected from the group of an acrylic group monomer solution and a mixed monomer solution containing a vinyl monomer for copolymerization with an acrylic group monomer as a main component; the filler is alumina trihydrate; and the antimicrobial agent comprises a complex of chitosan with silver or a silver compound.

BRIEF DESCRIPTION OF THE DRAWINGS

[0013] FIG. 1 shows the results of Corian® material with 0.5%, 1.0%, and 3.0% chitosan content vs. Escherichia coli (ATCC 25922).

[0014] FIG. 2 shows the results of Corian® material with 0.1%, 0.25%, 0.5%, and 1.0% chitosan-silver nitrate content vs. Escherichia coli (ATCC 25922).

[0015] FIG. 3 shows the results of Corian® material with 1% chitosan and 0.0237%, 0.0475%, 0.095%, 0.190% and 0.380% silver nitrate content vs. Escherichia coli (ATCC 25922). The respective chitosan to silver ratios as determined by ICP analysis are 1:0.016, 1:0.022, 1:0.05, 1:0.095, and 1:0.105.

[0016] FIG. 4 shows the results of Corian® material with 1% chitosan and 0.0237%, 0.0475%, 0.095%, 0.190%, and 0.380% silver nitrate content vs. Listeria weshimeri (ATCC 35897). The respective chitosan to silver ratios as determined by ICP analysis are 1:0.016, 1:0.022, 1:0.05, 1:0.095, and 1:0.105.

[0017] FIG. 5 shows the results of Corian® material with 1% chitosan and 0.0237%, 0.0475%, 0.095%, 0.190%, and 0.380% silver nitrate content vs. Candida albicans (ATCC 10231). The respective chitosan to silver ratios as determined by ICP analysis are 1:0.016, 1:0.022, 1:0.05, 1:0.095, and 1:0.105.

[0018] FIG. 6 shows the results of Corian® material with 1% chitosan and 0.0237%, 0.0475%, 0.095%, 0.190%, and 0.380% silver nitrate content vs. Staphylococcus aureus (ATCC 6538). The respective chitosan to silver ratios as determined by ICP analysis are 1:0.016, 1:0.022, 1:0.05, 1:0.095, and 1:0.105.

[0019] FIG. 7 shows the results of Corian® material with 1% chitosan and 0.0237%, 0.0475%, 0.095%, 0.190%, and 0.380% silver nitrate content vs. Escherichia coli (O157:H7). The respective chitosan to silver ratios as determined by ICP analysis are 1:0.016, 1:0.022, 1:0.05, 1:0.095, and 1:0.105.

[0020] FIG. 8 shows the results of Corian® material with 1% chitosan and 0.0237%, 0.0475%, 0.095%, 0.190%, and 0.380% silver nitrate content vs. Klebsiella pneumoniae (ATCC 4352). The respective chitosan to silver ratios as determined by ICP analysis are 1:0.016, 1:0.022, 1:0.05, 1:0.095, and 1:0.105.

[0021] FIG. 9 shows the results of Corian® material with 1% chitosan and 0.0237%, 0.0475%, 0.095%, 0.190%, and 0.380% silver nitrate content vs. Salmonella cholerasuis (ATCC 9239). The respective chitosan to silver ratios as determined by ICP analysis are 1:0.016, 1:0.022, 1:0.05, 1:0.095, and 1:0.105.

[0022] FIG. 10 shows the results of Corian® material with 1% chitosan and 0.0237%, 0.0475%, 0.095%, 0.190%, and 0.380% silver nitrate content vs. Escherichia coli (O157:H7) in the presence of BSA. The respective chitosan to silver ratios as determined by ICP analysis are 1:0.016, 1:0.022, 1:0.05, 1:0.095, and 1:0.105. Corian®AB™ material is an antimicrobial Corian® material containing silver zirconium phosphate and is used in this experiment as a putative positive control. The silver zirconium phosphate active was rendered inactive against this bacterium in the presence of BSA.

[0023] FIG. 11 shows the results of Corian® material with 1% chitosan and 0.0237%, 0.0475%, 0.095%, 0.190%, and 0.380% silver nitrate content vs. Escherichia coli (ATCC 25922) in the presence of BSA. The respective chitosan to silver ratios as determined by ICP analysis are 1:0.016, 1:0.022, 1:0.05, 1:0.095, and 1:0.105.

[0024] FIG. 12 shows the results of Corian® material with chitosan-zinc sulfate vs. Escherichia coli (ATCC 25922).

[0025] FIG. 13 shows the results of Corian® material with chitosan-zinc sulfate vs. Staphylococcus aureus (ATCC 6538).

[0026] FIG. 14 shows the results of Corian® material with chitosan-zinc sulfate vs. Candida albicans (ATCC 10231).

[0027] FIG. 15 shows the results of Corian® material with chitosan-copper sulfate vs. Escherichia coli (ATCC 25922).

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0028] The artificial marbles of the present invention are made from a curable resin composition containing a chitosan-metal complex as an antimicrobial agent. As used herein, by the term “complex” is meant a compound in which the bonding occurs by interaction of the electrons of the donor with the empty orbitals of the acceptor. In some complexes, the electron flow may take place in both directions simultaneously. (A New Dictionary of Chemistry, Fourth Edition, L. M. Miall and D. W. A. Sharo (eds.), John Wiley & Sons, Inc., New York, N.Y. (1968), p.157). The preferred embodiment of the invention comprises a chitosan-silver complex.

[0029] The artificial marble materials of this invention are effective in inhibiting or destroying many common harmful microorganisms encountered in the home, health care, and food preparation environments. Microorganisms commonly found in such environments, particularly when such environments remain wet, moist, or damp, include bacteria, yeasts, fungi, and viruses. Examples include, but are not limited to, Escherichia coli, Candida albicans, Staphylococcus aureus, Salmonella cholerasuis, Listeria weshimeri, and Klebsiella pneumoniae.

[0030] The present invention is directed to antimicrobial solid surfaces. By “antimicrobial” herein is meant bacteriocidal, fungicidal, and antiviral. The term “microbe” will similarly be used to mean a bacterium, fungus, or virus. The term “antimicrobial effectiveness” is intended to mean that, given a sufficient amount of antimicrobial agent, the microbial concentration of a sample is decreased by at least a 3-log factor (i.e., 99.9%) over a period of time. The actual antimicrobial effectiveness of an antimicrobial agent depends upon the specific resin matrix used and the specific bacteria tested.

[0031] The term “solid surface materials” herein refers to materials that are essentially non-porous composites of finely divided mineral fillers dispersed in an organic polymer matrix. As used herein, the term “organic polymer matrix” is synonymous with “resin matrix”. Solid surface materials include, for example, materials useful for decorative solid surfaces such as, for example, those used as building products such as bathroom vanity tops, sinks, shower stalls and kitchen countertops. Furniture, sanitary use, lining materials, and various articles such as office supplies and store fixtures may also be constructed of solid surface materials.

[0032] Solid surface materials comprise a resin matrix. The term “matrix” as used herein refers to the polymeric resin component in which fillers and other additives may be dispersed. The types of resin matrices useful in the present invention include thermoplastic resins, thermoset resins, and combinations thereof. Thermoplastic resins include olefins (such as low and high-density polyethylene and polypropylene), dienes (such as polybutadiene and Neoprene® elastomer), vinyl polymers (such as polystyrene, acrylics, and polyvinyl chloride), fluoropolymers (such as polytetrafluoroethylene), and heterochain polymers (such as polyamides, polyesters, polyurethanes, polyethers, polyacetals and polycarbonates). Thermoset resins include phenolic resins, amino resins, unsaturated polyester resins, epoxy resins, polyurethanes, and silicone polymers.

[0033] Epoxy resins useful in the present invention include epoxy resins of bisphenol type A, bisphenol type F, phenol novolak type, alicyclic epoxy, halogenated epoxy, and cycloaliphatic epoxy resins.

[0034] Unsaturated polyester resins useful in the present invention include those wherein the reactivity is based on the presence of double or triple bonds in the carbon atoms. Unsaturated polyester resins are formed by the reaction of molar amounts of unsaturated and saturated dibasic acids or anhydrides with glycols. The unsaturation sites can then be used to cross-link the polyester chains, via vinyl-containing monomers such as but not limited to styrene, MMA, or combinations of sytrene/MMA into a thermoset plastic state.

[0035] As is known to those of ordinary skill in the art, there can be many additives to epoxy or unsaturated polyesters. Typically, such materials are cured by adding cross-linking agents and catalysts to enhance the cross-linking action.

[0036] Acrylic resins useful in the present invention are not limited as long as the resin can be formed into an acrylic solid surface material by curing. Examples of useful acrylic resins include various kinds of conventional acrylic group monomers, acrylic group partial polymers, vinyl monomers for copolymerization other than acrylic group monomers, or partial polymers. As the acrylic group monomer, (meth)acrylic ester is preferable. As used herein, “(meth)acrylic” is understood to mean “acrylic and/or methacrylic”. Examples of (meth)acrylic esters include methyl (meth)acrylic ester, ethyl (meth)acrylic ester, butyl (meth)acrylic ester, 2-ethylhexyl (meth)acrylic ester, benzyl (meth)acrylic ester, glycidyl (meth)acrylic ester.

[0037] An example of a useful solid surface material including acrylic resin is the Corian® material, which includes a poly(methyl methacrylate) (PMMA) resin with ATH as a filler. Another example of a useful solid surface material is Zodiaq® material, which comprises an unsaturated polyester (UPE) resin with a quartz or other silica filler. Both Corian® material and Zodiaq® material can contain pigments, reground self material in particulate form, and other additives as disclosed in U.S. Pat. Nos. 3,847,865 and 4,085,246, both incorporated by reference herein.

[0038] The solid surface materials of the present invention comprise at least one antimicrobial agent that is dispersed in the resin matrix of the solid surface material in an amount that provides the solid surface material with an antimicrobial effectiveness as measured at an outer surface. The term “dispersed” herein means that the antimicrobial agent of the invention is present throughout the bulk of the solid surface material of the invention and not just on the surface of the solid surface material. The antimicrobial agent is provided in an amount that results in antimicrobial effectiveness, i.e., a 3-log reduction in the number of microorganisms, within about 24 hours from application as measured by the “Antimicrobial Hard Surface Test” and “Antimicrobial Hard Surface Wipe Test” methods described below.

[0039] The amount of antimicrobial agent is preferably at least about 0.5 to 8% by weight of the precured total composition and, more preferably, at least about 1% by weight of the precured total composition. It is preferred that the antimicrobial agent be added and dispersed into the resin component. Chitosan-silver complex, for example, may be added to the MMA before polymerization. Chitosan-silver complex may be added to the UPE before mixing with quartz or other silica and then vibrocompacted. Further processing (polymerization) does not alter the antimicrobial features of the agent.

[0040] The antimicrobial agent comprises a complex of chitosan and a metal, preferably silver, copper, or zinc. The metal or metal compounds can be present in amounts of 1% to 14% by weight based on the chitosan. These materials were ground to about 400 mesh size for use as additives in the preparation of polymers. While 400 mesh size was used for the embodiments of the Examples, the range of the particle size may be from about 100 mesh and smaller. Chitosan-silver complex is preferred for its superior antimicrobial efficacy.

[0041] The chitosan-silver complex used in the present invention is prepared by slowly adding a solution of silver salt to a chitosan solution such that a clear, colorless gel results. Typically, the silver salt solution is 0.5 to 20 wt % silver nitrate in water. The chitosan solution comprises 0.25% to 8.0% by weight chitosan in a dilute (0.25 to 5.0% by volume) aqueous solution of acetic acid. Typically, the chitosan is a 0.75% or 1.5% by volume aqueous acetic acid solution containing 2% by weight chitosan.

[0042] When acidic aqueous solution is added to the chitosan-silver gel, a solution results that can be used, for example, as a finish. A solid form of the complex can be produced from the gel by a method comprising the following steps:

[0043] (i)adding water to the gel, with stirring;

[0044] (ii) raising the pH to the product of step (i) to pH 7 to 8 by adding a basic solution as is commonly known in the art;

[0045] (iii) filtering the product of step (ii)

[0046] (iv) washing the filtered solids with water, then with acetonitrile;

[0047] (v) drying the washed solids under vacuum; and

[0048] (vi) optionally, grinding the dried product to a fine powder.

[0049] Typically, deionized water is used throughout and the pH is raised in step (ii) by dropwise addition of aqueous ammonium hydroxide or substituted ammonium hydroxide.

[0050] As opposed to a heterogenous synthesis of chitosan-silver ion complex in which chitosan as an insoluble aqueous suspension is treated with a solution of silver nitrate (see, for example, “Characterization of Silver-binding Chitosan by Thermal Analysis and Electron Impact Mass Spectrometry,” C. Peniche-Covas, M. S. Jimenez, A. Nunez, Carbohydrate Polymers (1988), 9, 249-256), the homogenous synthesis demonstrated here affords fibrous material with excellent swelling properties suitable for hydrogel applications, for example, as the absorbent element in a diaper, incontinence garment, tampon, or sanitary napkin. In addition, the material can be reconstituted in solution and used as a finish solution for textiles applications as is commonly performed in the art, or added as a powder of desired particle size for the preparation of materials described herein. The material retains its integrity over long storage periods, for example, more than a year of shell life without becoming extremely colored.

[0051] Fillers useful in the present invention include, for example, alumina trihydrate (ATH), alumina monohydrate (AMH), Bayer hydrate (BayH), quartz and other forms of silica (SiO2), magnesium hydroxide (Mg(OH)2), calcium carbonate (CaCO3), barium sulfate (BaSO4) or decorative agents (e.g., mica, glass chips, clear acrylic chips, “color flop” pigments (pigments that change color as the angle of viewing changes)), as a list that is not exhaustive and not intended to limit the invention. Fillers can be present in amounts up to about 95% by weight. Typically, but not necessarily, the amount of filler is decreased by the weight percent of antimicrobial agent added.

[0052] Solid surface materials may also include functional or decorative additives such as pigments, dyes, flame retardant agents, parting agents, fluidizing agents, viscosity control agents, curing agents, antioxidants, and the like as may be known to those of ordinary skill in the art.

[0053] Solid surface materials of this invention are typically formed by casting into a sheet form or casting into a shape form such as a sink, for example. Solid surface materials of this invention can also be produced by, for example, compression molding, injection molding, extrusion, or vibrocompaction methods.

[0054] It is especially preferred that the solid surfaces of the present invention remain wet, damp or moist for optimum effectiveness. Examples of solid surfaces of the present invention include, but are not limited to, surfaces in home bathrooms, public restrooms, swimming pool areas, dormitories, stadiums, and athletic facilities: sinks, counter tops, shower walls and bases, and other walls that become wet during use. In medical care facilities, such as hospitals, clinics, medical vans, and nursing homes, the current invention provides antimicrobial protection in the form of surfaces for counter tops, sinks, shower walls and bases, and back splashes in, for example, patient rooms, laundry rooms, soiled linen areas, staff and visitor areas, intensive care and coronary care units.

[0055] The present invention is also useful for antimicrobial protection where there is indirect food contact with the solid surface. Some examples are: counter tops, sinks, back splashes, and table tops in kitchens; table tops, salad bar counters and shields, food lag areas, dirty dish areas, and dish washing and drying areas in restaurants and fast food establishments; certain areas in slaughterhouses where the nutrient insult is not excessive; table, counter top, and back splash areas in canning, freezing, red meat packing, and bread and pastry production facilities; and grocery and fresh food counter tops, displays, and other fixtures in a grocery store.

[0056] The present invention is also useful for the surfaces of writing instruments, such as pens and pencils, since pathogenic microorganisms are easily transmitted by hand contact, and perspiration would increase the antimicrobial efficacy.

[0057] Additional features of the invention are illustrated by the following Examples.

[0058] The meaning of abbreviations is as follows: “h” means hour(s), “min” means minute(s), “sec” means second(s), “d” means day(s), “μL” means microliter, “mL” means milliliters, “L” means liters, “μm” means micrometer, “ppm” means parts per million (i.e., milligrams per liter).

EXAMPLES

Testing Methods for Examples

[0059] The antimicrobial effectiveness of the various embodiments of this invention was evaluated by using the Antimicrobial Hard Surface Test Method and the Antimicrobial Hard Surface Wipe Test Method as described below:

Antimicrobial Hard Surface Test Method

[0060] The test is conducted using hard polymeric materials that are impregnated with an antimicrobial agent homogeneously dispersed throughout the entire thickness of the material (see U.S. Pat. No. 3,847,865 for Corian® material plaque preparation). Tiles of the test material are inoculated with a known density of microbial cells and incubated at controlled humidity to retard drying. Following standard microbiological techniques for enumerating microorganisms, significant efficacy is demonstrated when at least a 3-log reduction in cell density on test material compared to control material without antimicrobial agent is achieved.

[0061] The relationship between percent reduction and log reduction is conveniently seen by reference to the following:

Value % Reduction
1     90
2     99
3     99.9
4     99.99
5     99.999

Procedure

[0062] 1. In the chemical fume hood, buff/renew control and test Corian® 6×6 tiles by using either a maroon Scotch-Brite™ very fine abrasive pad (3M #7447) or 200 grit or finer sandpaper. In a biological safety cabinet, wipe each tile with an isopropanol wipe, place in a sterile deep petri plate (100×20 mm), air dry, and cover with the lid.

[0063] 2. From an overnight culture grown in Trypticase Soy Broth (TSB) at 25° C., prepare inoculum that is approximately 1×106 cfu (colony forming units)/ml phosphate buffer*. (Typically an overnight culture is diluted 1:1,000 in phosphate buffer to yield this density.) Determine the final cell density by performing a serial-dilution spread plate count of the inoculum on Trypticase Soy Agar (TSA).

[0064] 3. Inoculate each tile by placing 0.5 mL of inoculum on the surface and spreading evenly with a sterile glass or plastic spreader. The inoculum should not go over the edge of the tile, but should remain on the “test side”. Put the lid on the petri plate and place in an open tray. Incubate in an environmental chamber at 25° C. and 85% relative humidity (% RH).

[0065] 4. To determine speed of kill (i.e., time required to achieve a 3-log or 99.9% reduction) for the antimicrobial tiles, generate a time-curve by incubating for 1, 2, 3, 4, 6, and 8 h. After the designated incubation/exposure time, remove the petri plates from the environmental chamber. In the biological safety cabinet, remove the petri dish lid and rinse the tile twice with phosphate buffer using a sterile 5 mL pipet. Use 4.5 mL for the first rinse and 5.0 mL for the second rinse. It is critical to rinse the tile by repeatedly sucking and expelling the buffer as the pipet is moved across the entire tile test surface. After the last rinse, thoroughly wipe the surface with a sterile 1 inch square gauze pad. Place the gauze into a sterile test tube along with the buffer rinses.

[0066] 5. Determine the bioburden of the rinse buffer using a phosphate buffer serial-dilution spread plate technique on TSA. Incubate the plates at the optimal growth temperature and conditions for the test microorganism for at least 24 h. Count the colonies on plates and calculate the density taking into account all dilutions. Report the findings as cfu/mi.

[0067] 6. The Δt value may be calculated as follows: Δt=C−B, where Δt is the activity constant for contact time t, C is the mean log10 density of microbes rinsed off of control tiles after X hours of incubation, and B is the mean log10 density of microbes rinsed off of test tiles after X hours of incubation.

[0068] *Stock Phosphate Buffer:

	Monobasic Potassium Phosphate	22.4	g
	Dibasic Potassium Phosphate	56.0	g
	Deionized Water	to 1000	mL

[0069] Adjust to pH 6.0-7.0 with either NaOH of HCl, filter, sterilize, and store at 4° C. until use.

[0070] Working Phosphate Buffer:

[0071] Dilute 1 mL of stock phosphate buffer in 800 mL of sterile deionized water (pH should be 6.0-7.0), dispense in working volumes and autoclave.

Antimicrobial Hard Surface Wipe Test Method

Summary of Method

[0072] This test is used to determine the frequency of renewal required for an antimicrobial surface that can be regenerated by buffing with an abrasive pad or sandpaper. The experimental design described below can be used to determine the duration of antimicrobial efficacy under normal use conditions. A surface with “reduced activity” is one in which the antimicrobial activity has fallen below a 3-log reduction capability.

[0073] Wiping With Damp Cloth: Soapy Water

[0074] The purpose of this protocol is to determine the effect of repeated typical clean-ups with soapy water on the durability of the efficacy of antimicrobial surfaces.

[0075] 1. Prepare a set of control and test tiles as described in the “Antimicrobial Hard Surface Test Method”.

[0076] 2. Wipe each tile set with a sterile cloth (e.g. cheesecloth, typical cotton kitchen towel, sponge, pre-moistened wipe, etc.) dampened with soapy water. The preparation of the soapy water is per the soap manufacturer's label instructions. Completely soak the cloth in the soapy water and hand wring prior to each use. A back and forth motion is used to completely wipe the surface of each tile.

[0077] 3. After each wipe, rinse the tile with sterile deionized water to remove any soap residue and air dry.

[0078] 4. After each set of 50 wipes, test the control and test tiles for antimicrobial efficacy using the “Antimicrobial Hard Surface Test Method”.

[0079] 5. Continue test in sets of 50 wipes until either an expected use period is satisfied or until the antimicrobial surface shows reduced activity. When Δt<3.0, the test tile is considered to have reduced activity.

[0080] Wiping With Damp Cloth: Liquid or Spray Disinfectant/Sanitizer

[0081] The purpose of this protocol is to determine the effect of repeated typical clean-ups with liquid disinfectants or sanitizers on the durability of the efficacy of antimicrobial surfaces.

[0082] 1. Prepare a set of control and test tiles as described in the “Antimicrobial Hard Surface Test Method”.

[0083] 2. Manufacturers use directions for each disinfectant/sanitizer are not consistent. In order to standardize the exposure conditions, use the following directions. For liquid products, completely soak a sterile cloth in the disinfectant/sanitizer solution prepared according to the manufacturer's label directions and hand wring prior to each use. Wipe each tile with a back and forth motion to completely cover the surface of each tile. For spray products, spray the tile surface twice to ensure a thorough wetting and wipe once using a back and forth motion with a sterile cloth.

[0084] 3. After each set of 50 wipes, test the control and test tiles for antimicrobial efficacy using the “Antimicrobial Hard Surface Test Method”.

[0085] 4. Continue test in sets of 50 wipes until either an expected use period is satisfied or until the antimicrobial surface shows reduced activity. When Δt<3.0, the test tile is considered to have reduced activity.

Example 1

Preparation of Chitosan-Silver Nitrate Complexes

[0086] Chitosan (42 g, Chitoclear™ Foodgrade, Primex, Iceland) was dissolved in 2% aqueous acetic solution (1100 mL) and stirred vigorously. A solution of silver nitrate (30 g) in deionized water (100 mL) was added over a period of 10 min. A clear, thick gel resulted. Additional water (300 mL) was added to the gel and stirred for 30 min. Concentrated ammonium hydroxide was added in drops to raise pH to 7-8. The product was filtered, washed with water (4×500 mL), and then with acetonitrile (4×500 mL). The resulting product was dried under vacuum for two days, ground to a fine powder, and used as such in the Corian® AB™ material preparation. Yield of the product was 53.7 g. The amount of silver in the complex was determined by Inductively Coupled Plasma spectroscopy (ICP), which is an atomic emission spectroscopy method in which inductively coupled plasmas are used as the excitation source (see, for example, Inductively Coupled Plasma Emission Spectroscopy, pt. 1, P. W. J. M. Boumans, John Wiley & Sons (New York, N.Y.), 1987, pp. 2-3). ICP silver metal analysis of this material indicated the proportion of silver to be 13.5% by weight.

[0087] In contrast, when a chitosan/silver complex was prepared by treating a suspension of chitosan with silver nitrate solution, the resultant product visually appeared the same as the starting material, did not form a gel, and had not dissolved in deionized water even after two days. The absence of swelling of this preparation clearly indicates the lack of cross linking of chitosan chain by silver and it is likely that the metal is distributed more on the surface of the chitosan than dispersed within it.

Example 2

Preparation of Chitosan-Silver Nitrate Complexes With Varying Silver Content

[0088] Five solutions of chitosan (20 g each, Chitoclear™, Primex, Iceland) in 500 mL of water containing 7.5 mL of acetic acid were treated successively with aqueous solutions (50 mL) of silver nitrate in the following proportions. Solution A in 7.2 g, B=3.6 g, C=1.8 g, D=0.9 g, E=0.45 g of silver nitrate. The reaction was conducted and processed as described in the previous Example. Yield of the products 1A through 1E ranged from 25 to 30.0 g.

[0089] Silver Content by ICP Analysis:

[0090] 1A=10.5% silver

[0091] 1B=9.5% silver

[0092] 1C=5.1% silver

[0093] 1D=2.2% silver

[0094] 1E=1.6% silver

[0095] In the following Examples 3-5, Corian® material plaques, 6 cm by 6 cm by about 1.3 cm, containing additives as indicated, were prepared according to U.S. Pat. No. 3,847,865.

Example 3

[0096] In the first plaque preparation, plain chitosan powder (Chitoclear™, Primex, Iceland) in 0.5%, 1.0%, and 3% concentrations by weight were added to the Corian® material mix and cast into plaques. As shown in FIG. 1, no significant antimicrobial activity was observed for these samples.

Example 4

[0097] Chitosan-silver nitrate powder from Example 1 was added to the plaque mixtures in 0.1%, 0.25%, 0.5%, and 1.0% concentrations by weight. The effective concentrations of the silver in these samples based on the additives were 0.01%, 0.03%, and 0.13%, respectively. These plaques exhibited effective antimicrobial activity as shown in FIG. 2.

Example 5

[0098] The following five Corian® material plaques were made as described in Example 4, except the chitosan concentration in all of these preparations was maintained at 1% by weight and the amount of silver nitrate relative to chitosan was changed using the material described in Example 2. Thus, the amounts of chitosan-silver in samples A to E respectively, were: 1:0.105; 1:0.095; 1:0.05; 1:0.022; 1:0.016. All these plaques exhibited bactericidal activity against a variety of organisms as shown in FIGS. 3 through 9.

[0099] In addition, these chitosan-silver Corian® material plaques maintained antimicrobial activity against Escherichia coli O157:H7 (FIG. 10), a microbe that is difficult to kill, and against Escherichia coli ATCC 25922 (FIG. 11) in the presence of “soil”. Bovine serum albumin (BSA) was added at 1.15 g per liter of phosphate buffer and utilized to prepare the inoculum as described for the “Antimicrobial Hard Surface Test Method”. This is a significant finding since many antimicrobial surfaces are inactivated in the presence of “soil”, as can be seen with the Corian® AB™ material positive control that was rendered ineffective against E. coli O157:H7 (FIG. 10).

Example 6

Preparation of Chitosan-Zinc Sulfate for Use as Additives in Corian® Material Plaques

[0100] Chitosan (40.5 g, Chitoclear™, Primex, Iceland) was dissolved in 2% aqueous acetic acid (1000 mL) and was vigorously stirred. To this, a solution of zinc sulfate (44.0 g) in water (100 mL) was added in drops. A viscous solution was obtained. To this, 250 mL of acetone was added to precipitate the product, which was filtered, washed with deionized water, and acetonitrile. It was dried under vacuum and ground to a fine powder (about 400 mesh size; 64 g). This preparation provided antimicrobial plaques against Gram positive and Gram negative bacteria as well as against yeasts as indicated in FIGS. 12, 13, and 14.

Example 7

Preparation of Chitosan-Copper Sulfate Complexes for Incorporation into Corian® Material Plaques

[0101] Chitosan (20.0 g, Chitoclear™, Primex, Iceland) was dissolved in 1.5% aqueous acetic acid (650 mL) and was vigorously stirred. To this, a solution of copper sulfate (25.0 g) in water (140 mL) was added in drops. A fibrous precipitate was obtained, which was filtered, washed with deionized water, and acetonitrile. It was dried under vacuum and ground to a fine powder (42 g).

[0102] Corian® material plaques containing 0.5%, 1.0%, 2.0%, and 3.0% concentration by weight of the chitosan-copper sulfate powder were prepared and evaluated for their antimicrobial properties (FIG. 15).

Claims

1. A solid surface material exhibiting antimicrobial effectiveness, the solid surface material comprising a matrix of a resin selected from thermoplastic resins, thermoset resins, and combinations thereof; and at least one antimicrobial agent dispersed in the matrix, wherein the antimicrobial agent comprises a complex selected from chitosan and a metal and chitosan and a metal compound.

2. The solid surface material of claim 1, further comprising at least one filler dispersed in the matrix.

3. The solid surface material of claim 1, wherein the thermoplastic resin is selected from olefins, dienes, vinyl polymers, fluoropolymers, heterochain polymers, and combinations thereof.

4. The solid surface material of claim 1, wherein the thermoplastic resin is selected from low density polyethylenes, high density polyethylenes, polypropylenes, polybutadienes, neoprenes, polystyrenes, acrylics, polyvinyl chloride, polytetrafluoroethylene, polyamides, polyesters, polyurethanes, polyethers, polyacetals, polycarbonates, and combinations thereof.

5. The solid surface material of claim 1, wherein the resin is made from a syrup comprising as the main component, an acrylic group polymer dissolved in an acrylic group monomer solution or a mixed monomer solution containing a vinyl monomer for copolymerization with an acrylic group monomer.

6. The solid surface material of claim 1, wherein the thermoset resin is selected from phenolic resins, amino resins, unsaturated polyester resins, epoxy resins, polyurethanes, silicone polymers, and combinations thereof.

7. The solid surface material of claim 2, wherein the filler is selected from the group consisting of alumina trihydrate, alumina monohydrate, Bayer hydrate, magnesium hydroxide, calcium carbonate, barium sulfate, and quartz and other forms of silica.

8. The solid surface material of claim 1, wherein the antimicrobial agent is present in an amount that provides an antimicrobial effectiveness measured at an outer surface within about 24 hours.

9. The solid surface material of claim 1, wherein said metal is silver, copper, zinc, or compounds thereof.

10. The solid surface material of claim 5 further comprising a filler comprising alumina trihydrate, and wherein the antimicrobial agent comprises a complex of chitosan with silver or a silver compound.

11. The solid surface material of claim 1 in the form of a sheet or a shaped article.

12. The solid surface material of claim 1 produced by compression molding, injection molding or extrusion.

13. A sink comprising the solid surface material of claim 1.

14. A counter top comprising the solid surface material of claim 1.

15. A tabletop comprising the solid surface material of claim 1.

16. A writing instrument comprising the solid surface material of claim 1.

17. A wall comprising the solid surface material of claim 1.

18. The wall of claim 17 wherein said wall is an element of a bathtub or a shower.

19. A shower base comprising the solid surface material of claim 1.

20. A method for reducing microbial growth on a solid surface in a bathroom, restroom, or powder room, the method comprising providing a wall, sink, vanity, counter, bathtub, shower stall, or table top which comprises the solid surface material of claim 1, wherein said solid surface material may be exposed to microorganisms that are commonly present in such a bathroom, restroom, or powder room.

21. A method for reducing microbial growth on a solid surface in a medical care facility, the method comprising providing a wall, sink, counter top, table, table top, shower stall, or back splash which comprises the solid surface material of claim 1, wherein said solid surface may be exposed to microorganisms that are commonly present in such a facility.

22. The method of claim 21 wherein the medical care facility is a hospital, clinic, medical van, or nursing home.

23. A method for reducing microbial growth on a solid surface in a facility for food display, preparation, service or handling, the method comprising providing a counter top, cutting board, sink, back splash, table top, salad bar top, salad bar shield, food lag area, dirty dish area, dish washing or dish drying area comprising the solid surface material of claim 1, wherein said solid surface material may be exposed to microorganisms that are commonly present in such food display, preparation, service, or handling facilities.

24. The method of claim 23 wherein the food preparation facility is selected from the group consisting of canning, freezing, meat packing, fish packing, bread baking, and pastry baking facilities.

25. A method for producing a chitosan-silver complex, the method comprising the sequential steps of: (a) dissolving 0.25 to 8.0% by weight chitosan in an acid solution; (b) adding a solution of a silver salt to the product of step (a); (c) adding water to the product of step (b), with stirring; (d) raising the pH of the product of step (c) to pH7 to 8 by adding a basic solution; (e) filtering the product of step (d); (f) washing the filtered solids obtained in step (e) with water, (g) washing the solids of step (f) with acetonitrile; (h) drying the washed solids under vacuum to obtain the chitosan-silver complex; and (i) optionally, grinding the dried product of step (h) to a fine powder.

26. The method of claim 25, wherein the acid solution is a 0.25 to 5% aqueous solution of acetic acid; the silver salt solution is 0.5 to 20wt % silver nitrate in water; and the pH is raised in step (d) by addition of aqueous ammonium hydroxide or substituted ammonium hydroxide.

27. A chitosan-silver complex produced by the method of claim 25.

28. A finish solution for textile applications comprising the chitosan-silver complex of claim 27.

29. A diaper, incontinence garment, sanitary napkin or tampon comprising the chitosan-silver complex of claim 27.