Patent Application
20220106308
This invention relates to novel antibacterial compounds grounded on innovative monocyclic fragments coupled to aminopiperidine naphthyridine scaffold, which can be used in treating bacterial infections. Herein disclosed novel antibacterial compounds can also be capable of treating bacterial infections caused by resistant bacterial strains, which nowadays are difficult to treat with the existing antibacterial chemotherapy.
The golden age of antibiotics in which more than two hundred antibacterials have been developed since penicillin has presented the mankind with the sense of security and the therapeutic area has been considered mature. Unfortunately, in the current era of enormous and uncontrolled use of antibacterials, we are witnessing a rapid grow in the antimicrobial resistance in general, making the effectiveness of these drugs highly questionable. This post a global health concern associated with alarmingly growing number of bacterial infections, which are no longer treatable with the available medicines. Bacterial infections caused by methicillin-resistant Staphylococcus aureus (MRSA), Streptococcus pneumoniae, Escherichia coli, Klebsiella pneumoniae, non-typhoidal Salmonella (NTS), Shigella species and Neisseria gonorrhoeae, have been exposed as an international problem to the health care by causing high proportions of resistance with increased risk for fatal clinical outcomes, which in turn requires treatments substantiated on expensive and intrusive drugs. The rapid development of drug resistance in microbes, however, has changed the situation in such a way that the research efforts directed toward the creation of a qualitatively new generation of antibacterials are stimulated both in pharma industry and academia. Put differently, there is an urgent need for development of an utterly new class of antibacterials that are previously not used in the treatment of bacterial infections.
The international publication number WO 1999/37635 discloses compounds, i.e., antibacterials grounded on aminopiperidine quinoline scaffold and connected to cyclic or acyclic moiety for the first time. Since then, a plethora of structurally similar compounds bearing antibacterial properties emerged in the patent publications, including WO 2000/21952, WO 2001/07432, WO 2001/07433, WO 2002/056882, WO 2002/08244, WO 2004/058144, WO 2006/020561, WO 2007/016610, and WO 2007/118130. While aminopiperidine linker bicyclic moiety prevailed as a pivotal component of the described compounds, a profusion of quinoline-like variants (e.g., napthyridines, quinoxalines, etc.) was introduced as well.
In contrast to bicyclic constructs, the present invention introduces novel compounds as antibacterials based on innovative monocyclic fragments coupled to aminopiperidine naphthyridine scaffold. By replacing bicyclic moiety of the existing antibacterials with monocyclic entities, we developed compounds with outstanding antibacterial activity and potency that outperform known antibacterial agents from the same class.
The present invention provides novel compounds grounded on monocyclic fragments coupled to aminopiperidine naphthyridine scaffold, pharmaceutical compositions comprising said compounds, and medical uses thereof.
The present invention can be summarized as follows:
The compounds of the present invention show high enzymatic inhibitory activity, high potency against various pathogens, and are useful as agents against bacterial infections.
Scheme 1. Synthesis of intermediate I2 and compounds C1-11.
Table 1. In vitro bacterial DNA gyrase inhibitory activity and human topo IIα selectivity.
Table 2. Antibacterial activity.
Table 3. Evaluation of the compound toxicity to the cells.
The present invention provides in first aspect a compound of Formula (I):
According to some embodiments, the compound according to the invention of general formula I is a compound wherein R1 is an unsubstituted monocyclic aryl.
According to some embodiments, the compound according to the invention of general formula I is a compound wherein R1 is a monocyclic aryl, substituted with one or more substituents selected from OH, halogen, CR3R4R5, NR3R4, OCR3R4R5 and COR6; wherein R3, R4 and R5 are independently selected from H, OH, halogen and (C1-6)alkyl, and R6 is selected from H, OH, halogen, (C1-6)alkyl and NR7R8, with R7 and R8 being independently selected from H, halogen and (C1-6)alkyl.
According to some embodiments, the compound according to the invention of general formula I is a compound wherein R1 is a monocyclic aryl, preferably phenyl, substituted with one or more substituents selected from OH, halogen, CH2OH, C(O)NH2, NH2, N(CH3)2, OCH3 and OCHR9R10 with R9 and R10 being each halogen.
According to some embodiments, the compound according to the invention of general formula I is a compound wherein R9 and R10 are each fluoro.
According to some embodiments, the compound according to the invention of general formula I is a compound wherein the monocyclic aryl is substituted in para, ortho and/or meta position.
According to some embodiments, the compound according to the invention of general formula I is a compound wherein the monocyclic aryl is mono-substituted.
According to some embodiments, the compound according to the invention of general formula I is a compound wherein the monocyclic aryl is mono-substituted at position 4.
According to some embodiments, the compound according to the invention of general formula I is a compound wherein the monocyclic aryl is di-substituted.
According to some embodiments, the compound according to the invention of general formula I is a compound wherein the monocyclic aryl is 3,4-di-substituted.
According to preferred embodiments, the compound according to the invention of general formula I is a compound wherein the monocyclic aryl is phenyl.
According to some embodiments, the compound according to the invention of general formula I is a compound wherein R1 is an unsubstituted monocyclic aromatic heterocycle.
According to some embodiments, the compound according to the invention of general formula I is a compound wherein R1 is an unsubstituted 5- or 6-membered monocyclic aromatic heterocycle.
According to some embodiments, the compound according to the invention of general formula I is a compound wherein R1 is an unsubstituted 5-membered monocyclic aromatic heterocycle.
According to some embodiments, the compound according to the invention of general formula I is a compound wherein R1 is a substituted monocyclic aromatic heterocycle.
According to some embodiments, the compound according to the invention of general formula I is a compound wherein R1 is a substituted 5- or 6-membered monocyclic aromatic heterocycle.
According to some embodiments, the compound according to the invention of general formula I is a compound wherein R1 is an unsubstituted 6-membered monocyclic aromatic heterocycle.
According to some embodiments, the compound according to the invention of general formula I is a compound wherein R1 is a substituted 5-membered monocyclic aromatic heterocycle.
According to some embodiments, the compound according to the invention of general formula I is a compound wherein the monocyclic aromatic heterocycle is substituted with one or more substituents selected from (C2-6)alkenyl, (C4-6)dienyl, (C2-6)alkynyl, and (C4-6)diynyl.
According to some embodiments, the compound according to the invention of general formula I is a compound wherein the monocyclic aromatic heterocycle is substituted with one or more substituents selected from ethenyl, propenyl, butenyl, pentenyl and hexenyl.
According to some embodiments, the compound according to the invention of general formula I is a compound wherein the monocyclic aromatic heterocycle is substituted with one or more substituents selected from vinyl, 2-propenyl, 3-butenyl, 4-pentenyl or 5-hexenyl.
According to some embodiments, the compound according to the invention of general formula I is a compound wherein the monocyclic aromatic heterocycle is substituted with 2-propenyl.
According to some embodiments, the compound according to the invention of general formula I is a compound wherein at least one N heteroatom (such as at least two N heteroatoms) in the heterocycle is substituted with one or more substituents selected from (C2-6)alkenyl, (C4-6)dienyl, (C2-6)alkynyl, and (C4-6)diynyl.
According to some embodiments, the compound according to the invention of general formula I is a compound wherein at least one N heteroatom (such as one, two or three N heteroatoms) in the heterocycle is substituted with one or more substituents selected from ethenyl, propenyl, butenyl, pentenyl and hexenyl.
According to some embodiments, the compound according to the invention of general formula I is a compound wherein at least one N heteroatom (such one, two or three N heteroatoms) in the heterocycle is substituted with one or more substituents selected from vinyl, 2-propenyl, 3-butenyl, 4-pentenyl and 5-hexenyl.
According to some embodiments, the compound according to the invention of general formula I is a compound wherein at least one N heteroatom (such one, two or three N heteroatoms) in the heterocycle is substituted with 2-propenyl.
According to some embodiments, the compound according to the invention of general formula I is a compound wherein at least two N heteroatoms (such as two or three N heteroatoms) in the heterocycle are substituted with one or more substituents selected from (C2-6)alkenyl, (C4-6)dienyl, (C2-6)alkynyl, and (C4-6)diynyl.
According to some embodiments, the compound according to the invention of general formula I is a compound wherein at least two N heteroatoms (such as two or three N heteroatoms) in the heterocycle are substituted with one or more substituents selected from ethenyl, propenyl, butenyl, pentenyl and hexenyl.
According to some embodiments, the compound according to the invention of general formula I is a compound wherein at least two N heteroatoms (such as two or three N heteroatoms) in the heterocycle are substituted with one or more substituents selected from vinyl, 2-propenyl, 3-butenyl, 4-pentenyl and 5-hexenyl.
According to some embodiments, the compound according to the invention of general formula I is a compound wherein at least two N heteroatom (such as two or three N heteroatoms) in the heterocycle are substituted with 2-propenyl.
According to some embodiments, the compound according to the invention of general formula I is a compound wherein the monocyclic aromatic heterocycle is substituted at position 1.
According to some embodiments, the compound according to the invention of general formula I is a compound wherein the monocyclic aromatic heterocycle is substituted at position 2.
According to some embodiments, the compound according to the invention of general formula I is a compound wherein the monocyclic aromatic heterocycle is mono-substituted.
According to some embodiments, the compound according to the invention of general formula I is a compound wherein the monocyclic aromatic heterocycle is mono-substituted at position 1.
According to some embodiments, the compound according to the invention of general formula I is a compound wherein the monocyclic aromatic heterocycle is mono-substituted at position 2.
According to some embodiments, the compound according to the invention of general formula I is a compound wherein the monocyclic aromatic heterocycle is mono-substituted at position 1 with a substituent selected from ethenyl, propenyl, butenyl, pentenyl and hexenyl.
According to some embodiments, the compound according to the invention of general formula I is a compound wherein the monocyclic aromatic heterocycle is mono-substituted at position 1 with a substituent selected from vinyl, 2-propenyl, 3-butenyl, 4-pentenyl or 5-hexenyl.
According to some embodiments, the compound according to the invention of general formula I is a compound wherein the monocyclic aromatic heterocycle is mono-substituted at position 1 with 2-propenyl.
According to some embodiments, the compound according to the invention of general formula I is a compound wherein the monocyclic aromatic heterocycle is mono-substituted at position 2 with a substituent selected from ethenyl, propenyl, butenyl, pentenyl and hexenyl.
According to some embodiments, the compound according to the invention of general formula I is a compound wherein the monocyclic aromatic heterocycle is mono-substituted at position 2 with a substituent selected from vinyl, 2-propenyl, 3-butenyl, 4-pentenyl or 5-hexenyl.
According to some embodiments, the compound according to the invention of general formula I is a compound wherein the monocyclic aromatic heterocycle is mono-substituted at position 2 with 2-propenyl.
According to some embodiments, the compound according to the invention of general formula I is a compound wherein the monocyclic aromatic heterocycle is an azole.
According to some embodiments, the compound according to the invention of general formula I is a compound wherein the monocyclic aromatic heterocycle has one, two or three N heteroatoms.
According to some embodiments, the compound according to the invention of general formula I is a compound wherein the monocyclic aromatic heterocycle has two or three N heteroatoms.
According to some embodiments, the compound according to the invention of general formula I is a compound wherein the monocyclic aromatic heterocycle has two N heteroatoms.
According to some embodiments, the compound according to the invention of general formula I is a compound wherein the monocyclic aromatic heterocycle is pyrazole, imidazole, 1,2,3-triazole or 1,2,4-triazole.
According to preferred embodiments, the compound according to the invention of general formula I is a compound wherein the monocyclic aromatic heterocycle is pyrazole.
According to some embodiments, the compound according to the invention of general formula I is a compound wherein the monocyclic aromatic heterocycle is 1H-pyrazol-4-yl substituted at position 1 with (C2-6)alkenyl.
According to preferred embodiments the compound according to the invention of general formula I is a compound wherein, R1 is independently
According to a preferred embodiment, the compound according to the invention of general formula I is a compound selected from the following list:
In another aspect the present invention provides the use of a compound selected from tert-butyl 1-(2-(6-methoxy-1,5-naphthyridin-4-yl)ethyl)piperidin-4-ylcarbamate and 1-(2-(6-methoxy-1,5-naphthyridin-4-yl)ethyl)piperidin-4-amine in the preparation of a compound according to the present invention as described above.
In another aspect the present invention provides a pharmaceutical composition comprising a compound according to the present invention as described above or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable carrier, adjuvant or vehicle. The present invention thus provides pharmaceutical compositions comprising a compound of this invention, or a pharmaceutically acceptable salt thereof together with a pharmaceutically acceptable carrier, adjuvant, or vehicle, for administration to a patient.
Examples of pharmaceutical compositions include any solid (tablets, pills, capsules, granules etc.) or liquid (solutions, suspensions or emulsions) composition for oral, topical or parenteral administration.
In a preferred embodiment the pharmaceutical compositions are in oral form, either solid or liquid. Suitable dose forms for oral administration may be tablets, capsules, syrups or solutions and may contain conventional excipients known in the art such as binding agents, for example syrup, acacia, gelatin, sorbitol, tragacanth, or polyvinylpyrrolidone; fillers, for example lactose, sugar, maize starch, calcium phosphate, sorbitol or glycine; tabletting lubricants, for example magnesium stearate; disintegrants, for example starch, polyvinylpyrrolidone, sodium starch glycollate or microcrystalline cellulose; or pharmaceutically acceptable wetting agents such as sodium lauryl sulfate.
The solid oral compositions may be prepared by conventional methods of blending, filling or tabletting. Repeated blending operations may be used to distribute the active agent throughout those compositions employing large quantities of fillers. Such operations are conventional in the art. The tablets may for example be prepared by wet or dry granulation and optionally coated according to methods well known in normal pharmaceutical practice, in particular with an enteric coating.
The pharmaceutical compositions may also be adapted for parenteral administration, such as sterile solutions, suspensions or lyophilized products in the appropriate unit dosage form. Adequate excipients can be used, such as bulking agents, buffering agents or surfactants.
The mentioned formulations will be prepared using standard methods such as those described or referred to in the Spanish and US Pharmacopoeias and similar reference texts.
Administration of the compounds or compositions of the present invention may be by any suitable method, such as intravenous infusion, oral preparations, and intraperitoneal and intravenous administration. Oral administration is preferred because of the convenience for the patient and the chronic character of the diseases to be treated.
Generally an effective administered amount of a compound of the invention will depend on the relative efficacy of the compound chosen, the severity of the disorder being treated and the weight of the sufferer. However, active compounds will typically be administered once or more times a day for example 1, 2, 3 or 4 times daily, with typical total daily doses in the range of from 0.1 to 1000 mg/kg/day.
The compounds and compositions of this invention may be used with other drugs to provide a combination therapy. The other drugs may form part of the same composition, or be provided as a separate composition for administration at the same time or at different time.
Another aspect of the invention refers to a compound of the invention according as described above according to general formula I, or a pharmaceutically acceptable salt thereof, for use as a medicament for the treatment or prophylaxis of a bacterial infection.
Another aspect of the invention refers to the use of a compound of the invention or a pharmaceutically acceptable salt thereof in the manufacture of a medicament.
Another aspect of the invention refers to the use of a compound of the invention in the manufacture of a medicament for the treatment or prophylaxis of a bacterial infection.
Another aspect of this invention relates to a method of treating or preventing a bacterial infection, comprises administering to a patient in need of such a treatment a therapeutically effective amount of a compound as above defined or a pharmaceutical composition thereof.
The term “halogen”, as used herein, includes fluoro, chloro, bromo, and iodo substituents.
The term “alkyl”, as used herein, refers to any linear and branched chains with number of carbons in specified range, for instance, methyl, ethyl, n-, iso-propyl, n-, iso-, sec-, t-butyl, pentyl and hexyl.
The term “alkenyl”, as used herein, refers to any linear and branched chains with number of carbons in specified range, for instance, vinyl, 2-propenyl, 3-butenyl, 4-pentenyl, 5-hexenyl.
The term “dienyl”, as used herein, refers to any linear and branched chains with number of carbons in specified range, for instance, 1,3-butadienyl, 1,3-pentadienyl, 1,3-hexadienyl, 1,5-hexadienyl, 3,5-hexadienyl.
The term “alkynyl”, as used herein, refers to any linear and branched chains with number of carbons in specified range, for instance, ethynyl, 2-propynyl, 3-butynyl, 3-pentynyl, 4-pentynyl, 5-hexynyl.
The term “diynyl”, as used herein, refers to any linear and branched chains with number of carbons in specified range, for instance, 1,3-butadiynyl, 1,3-pentadiynyl, 1,3-hexadiynyl, 1,5-hexadiynyl, 3,5-hexadiynyl.
The term “aryl”, as used herein, refers to ring systems with one aromatic ring but without heteroatoms even in only one of the rings. A preferred example of an aryl is phenyl.
The term “heterocycle”, as used herein, refers to an heterocyclic ring system with one aromatic containing one or more heteroatoms selected from the group consisting of nitrogen, oxygen and sulfur in the ring. A heterocyclic group can also be substituted once or several times. Preferably, the term “heterocycle”, as used herein, refers to 5- or 6-membered aromatic ring containing 1, 2 or 3 heteroatoms selected from N. More preferably, the term “heterocycle”, as used herein, refers to a 5-membered aromatic ring containing from 1, 2 or 3 heteroatoms selected from N. Examples of a heterocycle include pyrazole, imidazole, 1,2,3-triazole or 1,2,4-triazole. A preferred example is pyrazole. Heterocycle can be substituted with one substituent selected from (C2-6)alkenyl, (C4-6)dienyl, (C2-6)alkynyl, (C4-6)diynyl or their combinations.
The term “substituted with one or more substituents”, as used herein, refers to optional substitution with 1, 2, 3, 4, or 5 substituents, where all the substituents may be the same or different, or up to 4 substituents may be the same and the rest are different.
The compounds of the present invention can be found in the form of salts, such as pharmaceutically acceptable salts, solvates, hydrates, oxides, and pro-drugs, which are also covered within this invention and possess the desired activity and potency of the parent compound.
The term “salt” is to be understood as meaning any form of the active compound used according to the invention in which it assumes an ionic form or is charged and is coupled with a counter-ion (a cation or anion) or is in solution. By this are also to be understood complexes of the active compound with other molecules and ions, in particular complexes via ionic interactions.
The term “physiologically acceptable salt” means in the context of this invention any salt that is physiologically tolerated (most of the time meaning not being toxic—especially not caused by the counter-ion) if used appropriately for a treatment especially if used on or applied to humans and/or mammals.
Physiologically acceptable salts can also be formed with anions or acids and in the context of this invention is understood as meaning salts of at least one of the compounds used according to the invention as the cation with at least one anion which are physiologically tolerated—especially if used on humans and/or mammals. By this is understood in particular, in the context of this invention, the salt formed with a physiologically tolerated acid, that is to say salts of the particular active compound with inorganic or organic acids which are physiologically tolerated—especially if used on humans and/or mammals. Examples of physiologically tolerated salts of particular acids are salts of: hydrochloric acid, hydrobromic acid, sulfuric acid, methanesulfonic acid, formic acid, acetic acid, oxalic acid, succinic acid, malic acid, tartaric acid, mandelic acid, fumaric acid, lactic acid or citric acid.
The compounds of the invention may be present in crystalline form or in the form of free compounds like a free base.
Any compound that is a solvate of a compound according to the invention like a compound according to general formula I defined above is understood to be also covered by the scope of the invention. Methods of solvation are generally known within the art. Suitable solvates are pharmaceutically acceptable solvates. The term “solvate” according to this invention is to be understood as meaning any form of the active compound according to the invention in which this compound has attached to it via non-covalent binding another molecule (most likely a polar solvent). Especially preferred examples include hydrates and alcoholates, like methanolates or ethanolates.
Any compound that is a prodrug of a compound according to the invention like a compound according to general formula I defined above is understood to be also covered by the scope of the invention. The term “prodrug” is used in its broadest sense and encompasses those derivatives that are converted in vivo to the compounds of the invention. Such derivatives would readily occur to those skilled in the art, and include, depending on the functional groups present in the molecule and without limitation, the following derivatives of the present compounds: esters, amino acid esters, phosphate esters, metal salts sulfonate esters, carbamates, and amides. Examples of well known methods of producing a prodrug of a given acting compound are known to those skilled in the art and can be found e.g. in Krogsgaard-Larsen et al. “Textbook of Drug design and Discovery” Taylor & Francis (April 2002).
Unless otherwise stated, the compounds of the invention are also meant to include compounds which differ only in the presence of one or more isotopically enriched atoms. For example, compounds having the present structures except for the replacement of a hydrogen by a deuterium or tritium, or the replacement of a carbon by 13C- or 14C-enriched carbon or of a nitrogen by 15N-enriched nitrogen are within the scope of this invention.
The compounds of formula (I) as well as their salts or solvates of the compounds are preferably in pharmaceutically acceptable or substantially pure form. By pharmaceutically acceptable form is meant, inter alia, having a pharmaceutically acceptable level of purity excluding normal pharmaceutical additives such as diluents and carriers, and including no material considered toxic at normal dosage levels. Purity levels for the drug substance are preferably above 50%, more preferably above 70%, most preferably above 90%. In a preferred embodiment it is above 95% of the compound of formula (I), or of its salts. This applies also to its solvates or prodrugs.
This invention covers also all possible stereoisomers, geometric isomers, and tautomers of the compounds presented herein. Each compound can have one or more asymmetric centers and can therefore exist as individual diastereoisomers, or enantiomers, or as a mixture of stereoisomers. The term “geometric isomers” refers to compounds containing one or more double bonds, where cis or trans isomeric form can occur. Also included are tautomeric forms of the compounds that are able to interconvert.
Abbreviations
ATR attenuated total reflectance
DCM dichloromethane
DMSO dimethyl sulfoxide
ESI electrospray ionization
equiv equivalent
HPLC high-performance liquid chromatography
HRMS high-resolution mass spectrometry
IR infrared spectroscopy
MeOH methanol
Mp melting point
NMR nuclear magnetic resonance
Rf retention factor
UV ultraviolet
General Experimental Details:
All commercially available compounds were used without further purification. Anhydrous conditions were achieved using anhydrous solvents under inert atmosphere. Visualization with UV light and staining reagents on Merck silica gel (60 F254) plates (0.25 mm thick) was used for reaction progress and Rf determination. Column chromatography for compound purification was performed on silica gel 60 (particle size 0.040-0.063 mm; Merck). HPLC purity was analysed on Thermo Scientific DIONEX UltiMate 3000 instrument equipped with diode array detector using Acquity UPLC® BEH C8 column (1.7 μm, 2.1 mm×50 mm) by solvent system consisting of 0.1% trifluoroacetic acid in water (A) and acetonitrile (B), following the gradient: 90% A to 50% A in 6 min, then 50% A for 3 min, with flow rate 0.3 mL/min and injection volume 5 μL. All tested compounds showed ≥95% purity. Melting points were detected on a Reichert hot stage microscope and are uncorrected. 1H and 13C NMR spectra were recorded at 400 and 100 MHz, respectively, on a Bruker AVANCE III spectrometer (Bruker Corporation, Billerica, Mass., USA) in DMSO-d6 or CDCl3 solution using tetramethylsilane as internal standard. Mass spectra were recorded on Advion CMS spectrometer (Advion Inc., Ithaca, USA) or VG Analytical Autospec Q mass spectrometer (Fisons, VG Analytical, Manchester, UK). For obtaining IR spectra, Thermo Nicolet Nexus 470 ESP FT-IR spectrometer (Thermo Fisher Scientific, Waltham, Mass., USA) was used.
General Synthetic Scheme:
General Procedure for Reductive Amination:
To a solution of intermediate I2 (1 equiv) in dry methanol various aldehydes (1.05-1.3 equiv) were added, together with catalytic amount of acetic acid. The reaction mixture was stirred for 2 h at room temperature under inert atmosphere. Next, the reaction mixture was cooled to 0° C. and NaCNBH3 (3.5-4 equiv) dissolved in small amount of dry methanol was added drop-wise. After stirring overnight at room temperature under inert atmosphere, the solvent was evaporated in vacuum and the residue diluted in ethyl acetate (20 mL). Organic phase was washed with saturated Na2CO3 aqueous solution (3×10 mL), dried over Na2SO4, filtered and the solvent evaporated under reduced pressure. The residue was purified by flash column chromatography to afford appropriate product.
1-(2-(6-methoxy-1,5-naphthyridin-4-yl)ethyl)piperidin-4-amine
Tert-butyl 1-(2-(6-methoxy-1,5-naphthyridin-4-yl)ethyl)piperidin-4-ylcarbamate (I1) was prepared by method described in WO 2001/07432 A2.
I1 (0.63 g, 1.63 mmol, 1 equiv) was dissolved in 1 M HCl in acetic acid (12.5 mL) and stirred for 30 min at room temperature. Solvent was evaporated, the residue dissolved in saturated NaHCO3 (30 mL) and pH adjusted to 12 with 1M NaOH. Water layer was washed with DCM (3×30 mL), combined organic layers dried over Na2SO4 and concentrated in vacuum to afford intermediate I2 as dark-brown viscous liquid (0.330 g, 70.7%).
Rf: 0.10 (DCM/MeOH 4:1+1% Et3N);
1H NMR (400 MHz, CDCl3): δ=1.41-1.51 (m, 2H), 1.88 (d,J=11.9 Hz, 2H), 2.20 (t, J=11.0 Hz), 2.66-2.78 (m, 1H), 2.73-2.84 (m, 2H), 3.05 (d, J=11.5 Hz, 2H), 3.30-3.46 (m, 2H), 4.08 (s, 3H), 7.11 (d, J=9.0 Hz, 1H), 7.41 (d, J=4.5 Hz, 1H), 8.19 (d, J=9.0 Hz, 1H), 8.66 (d, J=4.5 Hz, 1H) ppm;
13C NMR (100 MHz, CDCl3): δ=28.46, 35.83, 52.37,53.72, 58.37, 77.23, 116.31, 124.24, 140.33, 140.97, 141.48, 146.64, 147.70, 161.43 ppm;
IR (ATR): v=3271, 2941, 2811, 1614, 1590, 1503, 1489, 1438, 1398, 1334, 1125, 1079, 1018, 932, 853, 807, 751, 713, 615, 584, 536 cm−1;
HRMS (ESI+) m/z for C16H22N4O14 ([M+H]30 ): calculated 287.1872, found 287.1875.
N-benzyl-1-(2-(6-methoxy-1,5-naphthyridin-4-yl)ethyl)piperidin-4-amine
Starting from I2 (0.2 g, 0.698 mmol, 1 equiv), benzaldehyde (0.092 mL, 0.908 mmol, 1.3 equiv) and NaCNBH3 (0.175 g, 2.792 mmol, 4 equiv), compound C1 was prepared according to the general procedure for reductive amination and purified with flash column chromatography using CHCl3/MeOH (9:1) to afford yellow solid (0.064 g, 24.4%).
Mp: 47.7-50.1° C.;
Rf: 0.28 (DCM/MeOH 4:1), 0.14 (CHCl3/MeOH 9:1);
1H NMR (400 MHz, CDCl3): δ=1.48-1.58 (m, 2H), 1.98-2.01 (m, 2H), 2.19-2.25 (m, 2H), 2.57-2.62 (m, 1H), 2.80-2.84 (m, 2H), 3.07-3.10 (m, 2H), 3.39-3.43 (m, 2H), 3.86 (s, 2H), 4.10 (s, 3H), 7.13 (d, J=8.8 Hz, 1H), 7.28-7.30 (m, 1H), 7.36 (d, J=4.4 Hz, 4H), 7.43 (d, J=4.4 Hz, 1H), 8.21 (d, J=9.2 Hz, 1H), 8.68 (d, J=4.4 Hz, 1H) ppm;
13C (100 MHz, CDCl3): δ=28.47, 32.80, 50.82, 52.35, 53.72, 54.09, 58.46, 77.24, 116.27, 124.24, 126.90, 128.06, 128.44, 140.34, 140.75, 140.99, 141.49, 146.75, 147.71, 161.40 ppm;
IR (ATR): v=2942, 2817, 1611, 1590, 1489, 1452, 1397, 1361, 1334, 1301, 1256, 1183, 1107, 1075, 1018, 987, 849, 808, 747, 697, 649, 614, 537 cm1;
MS (ESI+) m/z=377.3 (M+H)+;
HRMS (ESI+) m/z for C23H29ON4 ([M+H]+): calculated 377.2336; found 377.2334.
(4-((1-(2-(6-methoxy-1,5-naphthyridin-4-ypethyl)piperidin-4-ylamino)methyl)phenyl)methanol
Starting from I2 (0.2 g, 0.698 mmol, 1 equiv), 4-(hydroxymethyl)benzaldehyde (0.123 g, 0.908 mmol, 1.3 equiv) and NaCNBH3 (0.175 g, 2.792 mmol, 4 equiv), compound C2 was prepared according to the general procedure for reductive amination purified with flash column chromatography using CHCl3/MeOH (9:1) to afford brown solid (0.122 g, 43.0%).
Mp: 73.4-76.7° C.;
Rf: 0.07 (DCM/MeOH 4:1), 0.03 (CHCl3/MeOH 9:1);
1H NMR (400 MHz, CDCl3): δ=1.46-1.55 (m, 2H), 1.95-1.98 (m, 2H), 2.17-2.22 (m, 2H), 2.53-2.59 (m, 1H), 2.78-2.82 (m, 2H), 3.04-3.07 (m, 2H), 3.37-3.41 (m, 2H), 3.83 (s, 2H), 4.07 (s, 3H), 4.69 (s, 2H), 7.11 (d, J=8.8 Hz, 1H), 7.33 (s, 4H), 7.41 (d, J=4.8 Hz, 1H), 8.18 (d, J=8.8 Hz, 1H), 8.65 (d, J=4.4 Hz, 1H) ppm;
13C (100 MHz, CDCl3): δ=28.39, 32.48, 50.39, 52.24, 53.67, 53.79, 58.35, 65.04, 77.21, 116.32, 124.25, 127.20, 128.33, 139.79, 140.32, 140.96, 141.46, 146.62, 147.66, 147.70, 161.43 ppm;
IR (ATR): v=2938, 2821, 1611, 15920, 1503, 1487, 1446, 1397, 1333, 1257, 1174, 1104, 1073, 1050, 1017, 990, 930, 849, 805, 753, 613, 585 cm−1;
MS (ESI+) m/z=407.3 (M+H)+;
HRMS (ESI+) m/z for C24H31O2N4 ([M+H]+): calculated 407.2442, found 407.2440.
4-((1-(2-(6-methoxy-1,5-naphthyridin-4-yl)ethyl)piperidin-4-ylamino)methyl)benzamide
Starting from I2 (0.2 g, 0.698 mmol, 1 equiv), 4-formylbenzamide (0.135 g, 0.908 mmol, 1.3 equiv) and NaCNBH3 (0.175 g, 2.792 mmol, 4 equiv), compound C3 was prepared according to the general procedure for reductive amination and purified with flash column chromatography using CHCl3/MeOH (9:1) to afford yellow solid (0.033 g, 11.3%).
Mp: 116.1-122.1° C.;
Rf: 0.06 (DCM/MeOH 4:1), 0.02 (CHCl3/MeOH 9:1);
1H NMR (400 MHz, CDCl3): δ=1.44-1.54 (m, 2H), 1.94-1.96 (m, 2H), 2.14-2.20 (m, 2H), 2.51-2.56 (m, 1H), 2.77-2.81 (m, 2H), 3.04-3.07 (m, 2H), 3.36-3.40 (m, 2H), 3.90 (s, 2H), 4.07 (s, 3H), 5.59-6.17 (m, 2H), 7.11 (d, J=8.8 Hz, 1H), 7.41-7.44 (m, 3H), 7.78 (d, J=8.4 Hz, 2H), 8.18 (d, J=9.2 Hz, 1H), 8.66 (d, J=4.4 Hz, 1H) ppm;
13C (100 MHz, DMSO): δ=27.49, 32.05, 49.33, 51.67, 53.27, 53.58, 57.85, 115.99, 124.46, 127.20, 127.41, 132.32, 140.24, 140.45, 140.81, 144.74, 146.15, 147.66, 160.80, 167.10 ppm;
IR (ATR): v=3288, 3148, 2941, 2838, 1681, 1614, 1591, 1567, 1495, 1450, 1398, 1336, 1289, 1264, 1134, 1105, 1083, 1017, 990, 869, 851, 807, 782, 757, 691, 657, 631, 602, 586, 547 cm−1;
MS (ESI+) m/z=420.3 (M+H)+;
HRMS (ESI+) m/z for C24H30O2N5 ([M+H]+): calculated 420.2394, found 420.2391.
N-(4-(dimethylamino)benzyl)-1-(2-(6-methoxy-1,5-naphthyridin-4-yl)ethyl)piperidin-4-amine
Starting from 12 (0.2 g, 0.698 mmol, 1 equiv), 4-(dimethylamino)benzaldehyde (0.135 g, 0.908 mmol, 1.3 equiv) and NaCNBH3 (0.175 g, 2.792 mmol, 4 equiv), compound C4 was prepared according to the general procedure for reductive amination and purified with flash column chromatography using CHCL3/MeOH (9:1) to afford yellow-orange solid (0.196 g, 66.9%).
Mp: 89.4-91.6° C.;
Rf: 0.07 (DCM/MeOH 4:1), 0.05 (CHCl3/MeOH 9:1);
1H NMR (400 MHz, CDCl3): δ=1.47-1.57 (m, 2H), 1.95-1.98 (m, 2H), 2.15-2.21 (m, 2H), 2.56-2.62 (m, 1H), 2.76-2.80 (m, 2H), 2.93 (s, 6H), 2.98-3.09 (m, 2H), 3.35-3.39 (m, 2H), 3.74 (s, 2H), 4.07 (s, 3H), 6.71 (d, J=8.8 Hz, 2H), 7.11 (d, J=8.8 Hz, 1H), 7.21 (d, J=8.8 Hz, 2H), 7.41 (d, J=4.4 Hz, 1H), 8.18 (d, J=8.8 Hz, 1H), 8.66 (d, J=4.4 Hz, 1H) ppm;
13C (100 MHz, CDCl3): δ=28.43, 32.33, 40.74, 49.97, 52.26, 53.72, 58.39, 77.23, 112.71, 116.26, 124.24, 129.19, 140.31, 140.96, 141.46, 146.71, 147.67, 149.87, 161.39 ppm;
IR (ATR): v=2920, 2802, 1611, 1593, 1523, 1488, 1397, 2335, 1262, 1217, 1191, 1166, 1146, 1104, 1063, 1018, 958, 934, 856, 837, 809, 755, 712, 696, 647, 586, 566 cm−1;
MS (ESI+) m/z=420.3 (M+H)+;
HRMS (ESI+) m/z for C25H34ON5 ([M+H]+): calculated 420.2758, found 420.2754.
N-(4-fluorobenzyl)-1-(2-(6-methoxy-1,5-naphthyridin-4-yl)ethyl)piperidin-4-amine
Starting from I2 (0.233 g, 0.814 mmol, 1 equiv), 4-fluorobenzaldehyde (0.09 mL, 0.853 mmol, 1.05 equiv) and NaCNBH3 (0.179 g, 2.849 mmol, 3.5 equiv), compound C5 was prepared according to the general procedure for reductive amination and purified with flash column chromatography using CHCl3/MeOH (9:1+1% Et3N) to afford light brown solid (0.233 g, 69.2%).
Mp=55-60° C.;
Rf: 0.2 (DCM/MeOH 4:1+1% Et3N);
1C NMR (400 MHz, CDCl3): δ=1.44-1.55 (m, 2H), 1.95 (d, J=12.6 Hz, 2H), 2.18 (t, J=10.5 Hz, 2H), 2.46-2.62 (m, 1H), 2.74-2.86 (m, 2H), 3.05 (d, J=11.8 Hz, 2H), 3.31-3.44 (m, 2H), 3.80 (s, 2H), 4.07 (s, 3H), 6.97-7.04 (m, 2H), 7.11 (d, J=9.0 Hz, 1H), 7.27-7.33 (m, 2H), 7.41 (d, J=4.5 Hz, 1H), 8.18 (d, J=9.0 Hz, 1H), 8.66 (d, J=4.5 Hz, 1H) ppm;
13C NMR (100 MHz, CDCl3): δ=28.43, 32.71, 50.07, 52.32, 53.71, 58.41, 77.24, 115.20 (d, J=21.2 Hz), 116.29, 124.25, 129.54 (d, J=8.0 Hz), 136.41, 140.35, 140.97, 141.50, 146.67, 147.71, 161.42, 161.88 (d, J=244.6 Hz) ppm;
IR (ATR): v=3035, 2935, 2811, 1611, 1592, 1505, 1489, 1468, 1441, 1398, 1369, 1334, 1299, 1263, 1215, 1106, 1077, 1017, 932, 850, 812, 790, 751, 712, 696, 647, 607, 584, 564 cm−1;
HRMS (ESI+) m/z for C23H27FN4O ([M+H]+): calculated 395.2242; found 395.2238.
N-(4-chlorobenzyl)-1-(2-(6-methoxy-1,5-naphthyridin-4-yl)ethyl)piperidin-4-amine
Starting from I2 (0.2 g, 0.698 mmol, 1 equiv), 4-chlorobenzaldehyde (0.128 g, 0.908 mmol, 1.3 equiv) and NaCNBH3 (0.175 g, 2.792 mmol, 4 equiv), compound C6 was prepared according to the general procedure for reductive amination and purified with flash column chromatography using CHCl3/MeOH (9:1) to afford orange-brown solid (0.220 g, 76.7%).
Mp: 67.6-68.4° C.;
Rf: 0.44 (DCM/MeOH 4:1), 0.32 (CHCl3/MeOH 4:1);
1H NMR (400 MHz, CDCl3): δ=1.43-1.53 (m, 2H), 1.93-1.96 (m, 2H), 2.15-2.20 (m, 2H), 2.50-2.55 (m, 1H), 2.77-2.81 (m, 2H), 3.03-3.06 (m, 2H), 3.36-3.40 (m, 2H), 3.80 (s, 2H), 4.07 (s, 3H), 7.11 (d, J=8.8 Hz, 1H), 7.26-7.31 (m, 4H), 7.41 (d, J=4.4 Hz, 1H), 8.18 (d, J=8.8 Hz, 1H), 8.65 (d, J=4.4 Hz, 1H) ppm;
13C (100 MHz, CDCl3): δ=28.44, 32.69, 50.06, 52.29, 53.71, 54.04, 58.40, 77.25, 116.30, 124.74, 128.53, 129. 39, 132.56, 139.19, 140.32, 140.96, 141.47, 146.65, 147.69, 161.42 ppm;
IR (ATR): v=3223, 2945, 2827, 1490, 1262, 1092, 857, 810, 757, 641, 603 cm−1;
MS (ESI+) m/z=411.3 (M+H)+;
HRMS (ESI+) m/z for C23H28ON4Cl ([M +H]+): calculated 411.1946, found 411.1930.
N-(4-bromobenzyl)-1-(2-(6-methoxy-1,5-naphthyridin-4-yl)ethyl)piperidin-4-amine
Starting from I2 (0.2 g, 0.698 mmol, 1 equiv), 4-bromobenzaldehyde (0.168 g, 0.908 mmol, 1.3 equiv) and NaCNBH3 (0.175 g, 2.792 mmol, 4 equiv), compound C7 was prepared according to the general procedure for reductive amination and purified with flash column chromatography using CHCl3/MeOH (9:1) to afford yellow-orange solid (0.293 g, 92.2%).
Mp: 74.7-75.8° C.;
Rf: 0.55 (DCM/MeOH 4:1), 0.29 (CHCl3/MeOH 9:1);
1H NMR (400 MHz, CDCl3): δ=1.43-1.52 (m, 2H), 1.93-1.96 (m, 2H), 2.15-2.20 (m, 2H), 2.50-2.55 (m, 1H), 2.77-2.81 (m, 2H), 3.03-3.06 (m, 2H), 3.36-3.40 (m, 2H), 3.79 (s, 2H), 4.07 (s, 3H), 7.11 (d, J=8.8 Hz, 1H), 7.22 (d, J=6.32, 2H), 7.40-7.45 (m, 3H), 8.18 (d, J=8.8 Hz, 1H), 8.65 (d, J=4.4 Hz, 1H) ppm;
13C (100 MHz, CDCl3): δ=28.44, 32.69, 50.10, 52.29, 53.71, 54.03, 58.40, 77.25, 116.30, 120.63, 124.74, 129.77, 131.48, 139.72, 140.32, 140.97, 141.46, 146.66, 147.68, 161.42 ppm;
IR (ATR): v=3224, 2942, 2826, 1589, 1489, 1402, 1335, 1263, 1092, 1006, 856, 810, 757, 656, 634, 598, 545, 521 cm−1;
MS (ESI+) m/z=455.1 (M+H)+;
HRMS (ESI+) m/z for C23H28ON4Br ([M+H]+): calculated 455.1441, found 455.1424.
N-(4-iodobenzyl)-1-(2-(6-methoxy-1,5-naphthyridin-4-yl)ethyl)piperidin-4-amine
Starting from I2 (0.2 g, 0.698 mmol, 1 equiv), 4-iodobenzaldehyde (0.210 g, 0.908 mmol, 1.3 equiv) and NaCNBH3 (0.175 g, 2.792 mmol, 4 equiv), compound C8 was prepared according to the general procedure for reductive amination and purified with flash column chromatography using CHCl3/MeOH (9:1) to afford brown-yellow solid (0.285 g, 81.3%).
Mp: 80.8-82.7° C.;
Rf: 0.40 (DCM/MeOH 4:1), 0.17 (CHCl3/MeOH 9:1);
1H NMR (400 MHz, CDCl3): δ=1.43-1.52 (m, 2H), 1.92-1.95 (m, 2H), 2.14-2.20 (m, 2H), 2.50-2.55 (m, 1H), 2.77-2.81 (m, 2H), 3.03-3.06 (m, 2H), 3.36-3.40 (m, 2H), 3.78 (s, 2H), 4.07 (s, 3H), 7.08-7.12 (m, 3H), 7.41 (d, J=4.4 Hz, 1H), 7.41 (d, J=4.4 Hz, 1H), 7.64 (d, J=8.4 Hz, 2H), 8.18 (d, J=8.8 Hz, 1H), 8.66 (d, J=4.4 Hz, 1H) ppm;
13C (100 MHz, DMSO-d6): δ=28.42, 32.66, 50.17, 52.27, 53.72, 58.38, 77.24, 92.10, 116.30, 124.26, 130.07, 137.46, 140.36, 140.44, 140.96, 141.50, 146.60, 147.72, 161.43 ppm;
IR (ATR): v=2921, 2791, 1612, 1586, 1502, 1487, 1398, 1333, 1260, 1142, 1120, 1095, 1076, 1007, 930, 886, 855, 800, 737, 706, 627, 583, 538 cm−1;
MS (ESI+) m/z=503.2 (M+H)+;
HRMS (ESI+) m/z for C23H28ON4 ([M+H]+): calculated 503.1302, found 503.1298.
N-(4-(difluoromethoxy)-3-methoxybenzyl)-1-(2-(6-methoxy-1,5-naphthyridin-4-yl)ethyl)piperidin-4-amine
Starting from I2 (0.194 g, 0.677 mmol, 1 equiv), 4-(difluoromethoxy)-3-methoxybenzaldehyde (0.120 mL, 0.748 mmol, 1.1 equiv) and NaCNBH3 (0.149 g, 2.371 mmol, 3.5 equiv), compound C9 was prepared according to the general procedure for reductive amination and purified with flash column chromatography using CHCl13/MeOH (9:1+1% Et3N) to afford red-brown solid (0.194 g, 54.9%).
Mp=85-93° C.;
Rf: 0.19 (DCM/MeOH 4:1+1% Et3N);
1H NMR (400 MHz, CDCl3): δ=1.44-1.55 (m, 2H), 1.96 (d, J=11.6 Hz, 2H), 2.19 (t, J=10.5 Hz, 2H), 2.48-2.59 (m, 1H), 2.72-2.84 (m, 2H), 3.06 (d, J=11.7 Hz, 2H), 3.34-3.41 (m, 2H), 3.81 (s, 2H), 3.89 (s, 3H), 4.07 (s, 3H), 6.53 (t, J=75.4 Hz, 1H), 6.88 (dd, J1=8.1 Hz, J2=1.8 Hz, 1H), 7.01 (d, J=1.6 Hz, 1H), 7.10 (d, J=8.4 Hz, 1H), 7.11 (d, J=8.8 Hz, 1H), 7.41 (d, J=4.5 Hz, 1H), 8.18 (d, J=9.0 Hz, 1H), 8.66 (d, J=4.5 Hz, 1H) ppm;
13C NMR (100 MHz, CDCl3): δ=28.45, 32.79, 50.51, 52.36, 53.71, 55.91, 58.41, 77.23, 112.34, 116.25 (t, J=259.5 Hz), 116.29, 120.21, 122.22, 124.25, 138.73, 139.72, 140.37, 140.97, 141.50, 146.62, 147.72, 151.08, 161.42 ppm;
IR (ATR): v=2937, 2832, 1610, 1592, 1507, 1490, 1465, 1419, 1398, 1374, 1334, 1264, 1212, 1191, 1079, 1034, 1015, 853, 811, 787, 753, 732, 586 cm−1;
HRMS (ESI+) m/z for C25H30F2N4O3 ([M+H]+): calculated 473.2359; found 473.2354.
2-(difluoromethoxy)-5-((1-(2-(6-methoxy-1,5-naphthyridin-4-yl)ethyl)piperidin-4-ylamino)methyl)phenol
Starting from I2 (0.171 g, 0.597 mmol, 1 equiv), 4-(difluoromethoxy)-3-hydroxybenzaldehyde (0.138 g, 0.734 mmol, 1.2 equiv) and NaCNBH3 (0.131 g, 2.085 mmol, 3.5 equiv), compound C10 was prepared according to the general procedure for reductive amination and purified with flash column chromatography using CHCl3/MeOH (9:1+1% Et3N) to afford light brown solid (0.171 g, 50.9%).
Mp=99-104° C.;
Rf: 0.09 (DCM/MeOH 4:1+1% Et3N);
1H NMR (400 MHz, CDCl3): δ=1.50-1.61 (m, 2H), 1.99 (d, J=10.9 Hz, 2H), 2.28 (t, J=10.7 Hz, 2H), 2.60-2.68 (m, 1H), 2.83-2.89 (m, 2H), 3.12 (d, J=11.7 Hz, 2H), 3.38-3.43 (m, 2H), 3.75 (s, 2H), 4.07 (s, 3H), 6.51 (t, J=74.3 Hz, 1H), 6.79 (dd, J1=8.2 Hz, J2=1.9 Hz, 1H), 6.97 (d, J=1.9 Hz, 1H), 7.02 (d, J=8.2 Hz, 1H), 7.11 (d, J=9.0 Hz, 1H), 7.42 (d, J=4.5 Hz, 1H), 8.19 (d, J=9.0 Hz, 1H), 8.66 (d, J=4.5 Hz, 1H) ppm;
13C NMR (100 MHz, CDCl3): δ=28.12, 31.72, 49.94, 51.96, 53.77, 58.06, 77.23*, 116.38 (t, J=260.8 Hz), 116.46, 117.32, 119.91, 120.53, 124.34, 137.80, 138.54, 140.22, 140.89, 141.38, 146.10, 147.61, 148.08, 161.53 ppm;
IR (ATR): v=2944, 2338, 1612, 1595, 1490, 1438, 1399, 1334, 1297, 1261, 1113, 1076, 1049, 1014, 851, 809, 782, 752, 714, 674, 641, 586 cm−1;
HRMS (ESI+) m/z for C24H28F2N4O3 ([M+M]+): calculated 459.2202; found 459.2196.
N-((1-allyl-1H-pyrazol-4-yl)methyl)-1-(2-(6-methoxy-1,5-naphthyridin-4-yl)ethyl)piperidin-4-amine
Starting from 12 (0.2 g, 0.698 mmol, 1 equiv), 1-allyl-1H-pyrazole-4-carbaldehyde (0.112 g, 0.823 mmol, 1.2 equiv) and NaCNBH3 (0.166 g, 2.642 mmol, 3.8 equiv), compound C11 was prepared according to the general procedure for reductive amination and purified with flash column chromatography using CHCl3/MeOH (9:1+1% Et3N) to afford dark red viscous liquid (0.200 g, 59.8%).
Rf: 0.16 (DCM/MeOH 4:1+1% Et3N);
1H NMR (400 MHz, CDCl3): δ=1.50-1.61 (m, 2H), 2.00 (d, J=11.1 Hz, 2H), 2.30 (t, J=12.9 Hz, 2H), 2.60-2.71 (m, 1H), 2.83-2.91 (m, 2H), 3.11 (d, J=11.8 Hz, 2H), 3.37-3.46 (m, 2H), 3.74 (s, 2H), 4.08 (s, 3H), 4.71 (m, 2H), 5.19-5.31 (m, 2H), 6.01 (m, 1H), 7.12 (d, J=9.0 Hz, 1H), 7.43 (s, 3H), 8.19 (d, J=9.0 Hz, 1H), 8.66 (d, J=4.5 Hz, 1H) ppm;
13C NMR (100 MHz, CDCl3): δ=28.19, 31.71, 40.57, 51.98, 53.77, 54.79, 58.15, 77.23, 116.39, 118.78, 124.32, 128.19, 132.84, 139.02, 140.38, 140.90, 141.52, 147.74, 161.5 1 ppm;
IR (ATR): v=2938, 2805, 2324, 2167, 1612, 1589, 1489, 1439, 1398, 1372, 1333, 1260, 1119, 1077, 1018, 990, 928, 853, 807, 731, 642, 585 cm31 1;
HRMS (ESI+) m/z for C23H30N6O ([M+H]+): calculated 407.2554; found 407.2547.
1) Bacterial DNA Gyrase Inhibition Determination
Experimental Method:
The concentrations of the compounds needed to inhibit enzyme residual activity by half (IC50 values) were determined by Gyrase Supercoiling High Throughput Plate assay kit, purchased from Inspiralis (Norwich, UK) following their protocol. The method involved fluorescence measurements of enzyme activity at compound dilution series with seven concentrations, performed on black streptavidin-coated 96-wells microtiter plates. All example compounds were assayed against isolated Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli DNA gyrase enzyme, respectively, supplied as separate assay kits.
Results:
The results of IC50 values are given in Table 1 and correspond to the mean of two to four independent measurements. Ciprofloxacin was used as a reference compound with IC50 93.65 μM and 0.44 μM for S. aureus and E. coli, respectively.
2) Human Topoisomerase II Alpha Inhibition Determination
Experimental Method:
The selectivity of example compounds for bacterial DNA gyrase over the orthologous human topoisomerase IIα was determined by Human topoisomerase II Alpha Relaxation High Throughput Plate assay kit, purchased from Inspiralis (Norwich, UK) following their protocol. The method involved fluorescence measurements of enzyme activity at 10 μM compound concentration, performed on black streptavidin-coated 96-wells microtiter plates.
Results:
Selectivity of all example compounds was confirmed and the mean percentages of human topoisomerase IIα residual activity (% RA) of two independent measurements are given in Table 1.
In vitro bacterial DNA gyrase inhibitory activity
S. aureus
E. coli
3) Minimal inhibitory concentration (MIC) determination
Experimental Method:
Minimal inhibitory concentrations were determined by the broth microdilution method in 96-wells plate format following the Clinical and Laboratory Standards Institute (CLSI) guidelines given in “Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically, Approved Standard, 10th Edition, CLSI document M07-A10, Wayne, Pa.: Clinical and Laboratory Standards Institute, USA, 2015” and European Committee on Antimicrobial Susceptibility Testing (EUCAST) recommendations given in “The European Committee on Antimicrobial Susceptibility Testing, Breakpoint tables for interpretation of MICs and zone diameters, Version 5.0, 2015”. All exemplified compounds were tested on several Gram-positive (S. aureus, methicillin-resistant Staphylococcus aureus (MRSA), methicillin-resistant Staphylococcus schleiferi subspecies coagulans, methicillin-resistant Staphylococcus pseudintermedius (S-48, S-84, S-35, S-52, S-40 and S-51), Streptococcus agalactiae and Enterococcus faecalis) and Gram-negative bacteria (E. coli, Salmonella Alachua and Pseudomonas aeruginosa).
Results:
The MICs were visually determined as the lowest compound concentration inhibiting bacterial growth after 20 hours of incubation time. Tetracycline was used as a reference compound, showing MIC of 0.5 μg/mL and 1 μg/mL for S. aureus and E. coli, respectively. MIC values are represented in Table 2.
S.
S.
E.
agalactiae
Alachua
faecalis
S.
E.
aureus
coli
P.
C.
aeruginosa
A.
jejuni
S.
baumannii
pneumoniae
4) Cytotoxicity Assessment
Experimental Method:
Cytotoxicity was assessed in assay measuring metabolic cell activity performed on HepG2 (ATCC) and HUVEC (ATCC) cell lines according to the protocol of CellTiter 96® AQueous One Solution Cell Proliferation Assay 2018, Promega, USA, 2001. The method involved absorbance measurements after the treatment of cells with compounds at concentrations 1μM and 50 μM.
Results:
The results are given as the mean percentage of metabolic activity of treated cells from two independent experiments and are represented in Table 3.
| Number | Date | Country | Kind |
|---|---|---|---|
| LU101131 | Feb 2019 | LU | national |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/EP2020/054221 | 2/18/2020 | WO | 00 |
