Claims
- 1. An antibiotic AM-5344-A.sub.2 substance having the following physical and chemical properties:
- (1) Elemental Analysis (by weight): C: 63.87%; H: 3.96%; N: 2.29%;
- (2) Specific Rotary Power: [.alpha.].sub.D.sup.20 =-214.degree. (C=0.25, chloroform)
- (3) Melting point: more than 290.degree. C.;
- (4) Ultraviolet Absorption Spectrum: Shown in FIG. 1;
- (5) infrared Absorption Spectrum: Shown in FIG. 2;
- (6) Rf Values on Silica Gel Thin Layer Chromatography (TLC aluminum sheet and silica gel 60F.sub.254 produced by Merck & Co.; thickness: 0.2 mm): 0.32 in chloroform/methanol (40/1); 0.68 in benzene/acetone (1/1); 0.58 in benzene/methanol (4/1); 0.20 in ethyl acetate; and 0.61 in n-butanol/acetic acid/water (4/1/1);
- (7) Solubility: Soluble in chloroform; sparingly soluble in acetone, ethyl acetate; and benzene, and lower alcohols; and insoluble in n-hexane, ethyl ether, and water.
- 2. A process for preparing an antibiotic AM-5344-A.sub.2 substance as defined in claim 1, comprising cultivating as an antibiotic AM-5344-A.sub.2 substance-producing strain, Streptomyces sp. AM-5344 having FERM designation FERM BP-67, in a nutrient medium under aerobic stirring conditions at a temperature of 25.degree. to 30.degree. C. and at a pH of 6 to 7 for about 50 to 100 hours, and recovering the antibiotic AM-5344-A.sub.2 substance from the culture.
- 3. A process as in claim 2 wherein the antibiotic AM-5344-A.sub.2 substance is recovered from the culture by extracting with acetone, ethyl acetate, chloroform or benzene and isolating by column chromatography.
Priority Claims (1)
Number |
Date |
Country |
Kind |
55-177107 |
Dec 1980 |
JPX |
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Parent Case Info
This application is a continuation-in-part of Ser. No. 329,463, filed Dec. 10, 1981, now abandoned.
Non-Patent Literature Citations (1)
Entry |
Chemical Abstracts, 97:180143h (1982). |
Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
329463 |
Dec 1981 |
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